LOCUS       DAZUKA010000001        61341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000001 DAZUKA010000000
VERSION     DAZUKA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.001"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..1611
                     /locus_tag="ACG8PN_000001"
     CDS             139..1611
                     /locus_tag="ACG8PN_000001"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4860189.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            1623..2447
                     /gene="nadE"
                     /locus_tag="ACG8PN_000002"
     CDS             1623..2447
                     /gene="nadE"
                     /locus_tag="ACG8PN_000002"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4860190.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(2520..3410)
                     /locus_tag="ACG8PN_000003"
     CDS             complement(2520..3410)
                     /locus_tag="ACG8PN_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4860191.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            3608..5125
                     /locus_tag="ACG8PN_000004"
     CDS             3608..5125
                     /locus_tag="ACG8PN_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4860192.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5097..6386
                     /locus_tag="ACG8PN_000005"
     CDS             5097..6386
                     /locus_tag="ACG8PN_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4860193.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            6561..7457
                     /locus_tag="ACG8PN_000006"
     CDS             6561..7457
                     /locus_tag="ACG8PN_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860194.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            7471..8334
                     /locus_tag="ACG8PN_000007"
     CDS             7471..8334
                     /locus_tag="ACG8PN_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860195.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            8340..8903
                     /locus_tag="ACG8PN_000008"
     CDS             8340..8903
                     /locus_tag="ACG8PN_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4860196.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            8925..10436
                     /locus_tag="ACG8PN_000009"
     CDS             8925..10436
                     /locus_tag="ACG8PN_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4860197.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            10448..11407
                     /locus_tag="ACG8PN_000010"
     CDS             10448..11407
                     /locus_tag="ACG8PN_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4860198.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            11493..12572
                     /locus_tag="ACG8PN_000011"
     CDS             11493..12572
                     /locus_tag="ACG8PN_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4860199.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(12824..14224)
                     /locus_tag="ACG8PN_000012"
     CDS             complement(12824..14224)
                     /locus_tag="ACG8PN_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4860200.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            14442..16103
                     /locus_tag="ACG8PN_000013"
     CDS             14442..16103
                     /locus_tag="ACG8PN_000013"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4860201.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16060..16758
                     /locus_tag="ACG8PN_000014"
     CDS             16060..16758
                     /locus_tag="ACG8PN_000014"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4860202.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            16774..18198
                     /gene="araA"
                     /locus_tag="ACG8PN_000015"
     CDS             16774..18198
                     /gene="araA"
                     /locus_tag="ACG8PN_000015"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4860203.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            18505..19665
                     /locus_tag="ACG8PN_000016"
     CDS             18505..19665
                     /locus_tag="ACG8PN_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4860204.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(19642..19782)
                     /locus_tag="ACG8PN_000017"
     CDS             complement(19642..19782)
                     /locus_tag="ACG8PN_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4860205.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            19783..21642
                     /locus_tag="ACG8PN_000018"
     CDS             19783..21642
                     /locus_tag="ACG8PN_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4860206.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            21752..22186
                     /locus_tag="ACG8PN_000019"
     CDS             21752..22186
                     /locus_tag="ACG8PN_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860207.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22445..22606)
                     /locus_tag="ACG8PN_000020"
     CDS             complement(22445..22606)
                     /locus_tag="ACG8PN_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860208.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22625..23647)
                     /locus_tag="ACG8PN_000021"
     CDS             complement(22625..23647)
                     /locus_tag="ACG8PN_000021"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4860209.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            23775..24128
                     /locus_tag="ACG8PN_000022"
     CDS             23775..24128
                     /locus_tag="ACG8PN_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4860210.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            24140..25306
                     /locus_tag="ACG8PN_000023"
     CDS             24140..25306
                     /locus_tag="ACG8PN_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4860211.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(25398..26018)
                     /locus_tag="ACG8PN_000024"
     CDS             complement(25398..26018)
                     /locus_tag="ACG8PN_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4860212.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(26210..26506)
                     /locus_tag="ACG8PN_000025"
     CDS             complement(26210..26506)
                     /locus_tag="ACG8PN_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4860213.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            26710..27360
                     /locus_tag="ACG8PN_000026"
     CDS             26710..27360
                     /locus_tag="ACG8PN_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4860214.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            27376..29103
                     /locus_tag="ACG8PN_000027"
     CDS             27376..29103
                     /locus_tag="ACG8PN_000027"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860215.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            29100..30992
                     /locus_tag="ACG8PN_000028"
     CDS             29100..30992
                     /locus_tag="ACG8PN_000028"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860216.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            31696..32565
                     /locus_tag="ACG8PN_000029"
     CDS             31696..32565
                     /locus_tag="ACG8PN_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4860217.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            32774..33799
                     /locus_tag="ACG8PN_000030"
     CDS             32774..33799
                     /locus_tag="ACG8PN_000030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4860218.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            33838..34788
                     /gene="manA"
                     /locus_tag="ACG8PN_000031"
     CDS             33838..34788
                     /gene="manA"
                     /locus_tag="ACG8PN_000031"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4860219.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            34882..36585
                     /locus_tag="ACG8PN_000032"
     CDS             34882..36585
                     /locus_tag="ACG8PN_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4860220.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            36587..38620
                     /locus_tag="ACG8PN_000033"
     CDS             36587..38620
                     /locus_tag="ACG8PN_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4860221.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIPKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            38631..39071
                     /locus_tag="ACG8PN_000034"
     CDS             38631..39071
                     /locus_tag="ACG8PN_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4860222.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(39271..40398)
                     /locus_tag="ACG8PN_000035"
     CDS             complement(39271..40398)
                     /locus_tag="ACG8PN_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860223.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(40401..41078)
                     /locus_tag="ACG8PN_000036"
     CDS             complement(40401..41078)
                     /locus_tag="ACG8PN_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4860224.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            41342..43108
                     /locus_tag="ACG8PN_000037"
     CDS             41342..43108
                     /locus_tag="ACG8PN_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4860225.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            43123..43560
                     /locus_tag="ACG8PN_000038"
     CDS             43123..43560
                     /locus_tag="ACG8PN_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4860226.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            43573..44724
                     /locus_tag="ACG8PN_000039"
     CDS             43573..44724
                     /locus_tag="ACG8PN_000039"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4860227.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(44786..45484)
                     /locus_tag="ACG8PN_000040"
     CDS             complement(44786..45484)
                     /locus_tag="ACG8PN_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4860228.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(45477..46829)
                     /locus_tag="ACG8PN_000041"
     CDS             complement(45477..46829)
                     /locus_tag="ACG8PN_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4860229.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(47052..48452)
                     /locus_tag="ACG8PN_000042"
     CDS             complement(47052..48452)
                     /locus_tag="ACG8PN_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4860230.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            48613..49350
                     /locus_tag="ACG8PN_000043"
     CDS             48613..49350
                     /locus_tag="ACG8PN_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4860231.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            49475..49954
                     /locus_tag="ACG8PN_000044"
     CDS             49475..49954
                     /locus_tag="ACG8PN_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4860232.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            49951..50718
                     /locus_tag="ACG8PN_000045"
     CDS             49951..50718
                     /locus_tag="ACG8PN_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4860233.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            50948..51517
                     /gene="lepB"
                     /locus_tag="ACG8PN_000046"
     CDS             50948..51517
                     /gene="lepB"
                     /locus_tag="ACG8PN_000046"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4860234.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(51621..52223)
                     /locus_tag="ACG8PN_000047"
     CDS             complement(51621..52223)
                     /locus_tag="ACG8PN_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4860235.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            52329..52559
                     /locus_tag="ACG8PN_000048"
     CDS             52329..52559
                     /locus_tag="ACG8PN_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860236.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            52550..52798
                     /locus_tag="ACG8PN_000049"
     CDS             52550..52798
                     /locus_tag="ACG8PN_000049"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4860237.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            52904..53488
                     /locus_tag="ACG8PN_000050"
     CDS             52904..53488
                     /locus_tag="ACG8PN_000050"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4860238.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            53491..54564
                     /gene="prfA"
                     /locus_tag="ACG8PN_000051"
     CDS             53491..54564
                     /gene="prfA"
                     /locus_tag="ACG8PN_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4860239.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            54557..55396
                     /gene="prmC"
                     /locus_tag="ACG8PN_000052"
     CDS             54557..55396
                     /gene="prmC"
                     /locus_tag="ACG8PN_000052"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4860240.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            55389..55568
                     /locus_tag="ACG8PN_000053"
     CDS             55389..55568
                     /locus_tag="ACG8PN_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860241.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            55590..56615
                     /locus_tag="ACG8PN_000054"
     CDS             55590..56615
                     /locus_tag="ACG8PN_000054"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4860242.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            56806..58050
                     /gene="glyA"
                     /locus_tag="ACG8PN_000055"
     CDS             56806..58050
                     /gene="glyA"
                     /locus_tag="ACG8PN_000055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4860243.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            58203..59150
                     /locus_tag="ACG8PN_000056"
     CDS             58203..59150
                     /locus_tag="ACG8PN_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4860244.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            59284..59913
                     /gene="upp"
                     /locus_tag="ACG8PN_000057"
     CDS             59284..59913
                     /gene="upp"
                     /locus_tag="ACG8PN_000057"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4860245.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(60052..60294)
                     /locus_tag="ACG8PN_000058"
     CDS             complement(60052..60294)
                     /locus_tag="ACG8PN_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860246.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            60415..60487
                     /locus_tag="ACG8PN_000059"
     tRNA            60415..60487
                     /locus_tag="ACG8PN_000059"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60448..60450,aa:Thr,seq:cgt)
     gene            complement(60606..61244)
                     /locus_tag="ACG8PN_000060"
     CDS             complement(60606..61244)
                     /locus_tag="ACG8PN_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4860247.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        20374 a        10737 c        12686 g        17544 t
ORIGIN      
        1 gcagatggag ttcattttta gcaagaaata tccgtaaaac atgtgctttt tcagaaaaaa
       61 tggaagattt cttttcctat taattttttt tgttataatg ggtaggaaat gaaaacttgc
      121 caagaggagt aatacaagat gaataatact tacccagacg acagtttgac gctgcatacg
      181 gatctatatc aaatcaatat gatgcagaca tactgggaat taggtcgagc tgatttacat
      241 gctgtttttg aatgttactt tcgagaaatg ccttttaacc acggatacgc ggtttttgca
      301 ggattggaac ggttagtcaa ttatttagag aatctgacgt tcaatgattc cgatattgct
      361 tacttgcgtg gattagaagt ctatcctgaa ggttttttgg agtatttaca aaattttgaa
      421 tttaaagcaa cagttcgttc tgctcgtgaa ggagaactag tttttgcaaa tgaaccatta
      481 atccaagtgg aagggccttt agcgcactgc caattagtag aaacagcact gttgaatatg
      541 gtcaatttcc aaacgttgat cgcgacaaaa gcagctcgta tcaaatctgt catcggagaa
      601 gatccactat tggaatttgg aactagacgg gcacaagaac tggatgcagc agtctgggga
      661 actagagcgg catatatcgg cggagcagat gcaacaagca atgtacgtgc tgggaagata
      721 tttgggatac cagctagcgg gacccatgct cattcgttag tccaatctta tggaaatgat
      781 tatgaagcat ttatggccta cgctaaaacc cataaagact gcgtgttctt agtcgacact
      841 tatgatacgt tgaaatcagg tgtgccaagt gcgattcgcg tggcgaaaga attaggtgac
      901 aaaatcaact tccaaggcgt acgcatcgac agtggagata tggcctatat atctaagcgt
      961 gttcgggaac aattagatgc agccggattt acggaagcaa aaatctatgc ttcgaatgac
     1021 ttggatgaag caacgatttt gaatttaaag atgcaaaaag ctaaaattga tgtatggggt
     1081 gttgggacaa aattgatcac agcctatgat cagccagccc ttggtgcagt attcaagcta
     1141 gtatcgatcg aagatgatga aggaaaaatg atggatacga tcaagttatc aagcaatgca
     1201 gaaaaagtaa ctactccagg gaaaaaacaa gtgtggcgca tcactcgaaa ttttgatggg
     1261 aaatctgaag gagactacgt gacattatgg gatgaagacc ctcgagaaga agaagctatc
     1321 ttcatgttcc atccagtgca tacgtttatc aataaaactg ttcgagactt tacagctcgc
     1381 ccagttttac aagatatctt tatcgaagga aaaagagttt acgaattacc tgcactaaac
     1441 gaaatcaaag aatatacaaa agaaaatcta gattcactat gggaagagta taaacgagac
     1501 ttgaatccgc aaaaataccc agtcgatctg tctactgaat gctggaacca taaaatggct
     1561 atcatggaac gaatgaaaaa aagcgtagca gaacttcata cagaagcata ggaggagaat
     1621 aaatgagcct gcagacagaa attatcaatg aattaggtgt caaaccaaca attgatccaa
     1681 aagaagaaat acgtaaaagt attgacttta tgaaagcata tttgaaaaaa tatccgtttc
     1741 ttaaaacttt tgtactaggt atcagcggcg gccaagattc tactttagca ggccgtttgg
     1801 cgcaactaac gatggaagaa atgcgtgaag aaacaaaaga tgaaaactat caatttatcg
     1861 ctgttcgtct tccttatggt gaacaagcag atgaagaaga tgcaaaagct gctcttgact
     1921 ttatccagcc cgatgttagt ctgcgagtaa atatcaaacc agctgttgat gcacaagtac
     1981 aagtattaag cgaagcaggt gtgggaatca gcgattttaa caaaggaaat atcaaagcac
     2041 gtcaacgtat gattacgcaa tatgccgtag ctggagaaag agcaggagcc gtattaggga
     2101 cagatcatgc tgcagaaaac attactggat tcttcacgaa atttggtgat ggtggagccg
     2161 acattttgcc attattccgt ttggacaaac gacaagggaa acaattgctt caagcattga
     2221 atgcaccgga aaaattatac acaaaaattc cgactgcaga tcttgaagat gggaaaccaa
     2281 tgattgcaga tgaagtcgca ctaggtgtca catataatga aattgatgat tatctagaag
     2341 gaaaagaagt tcccgctcaa gcacaagaaa aaatcgaagc atggtggaac aagacacaac
     2401 ataaacgaca tttgccaatt tccgttttgg atgatttctg gaaataacaa taaagcaaaa
     2461 acagccgtcc cttcataggg attggctgtt tttgttttat aaaatagaaa agaaaaatat
     2521 cagctgtagt aaacttttaa tttgatatcc tgatcgtaat taccgaatcc tttaccaaat
     2581 aaagtacagc cccccacatg tttggcatcg tctttcactt cgattcgcaa atgccaagtg
     2641 tcttgctctt tagggatgtc gttgatcgtg atgtttgaca agggttctcc attcatattc
     2701 gtcaagtgtt tggtaattcg gatcgtcttc agcaagccgt attgattaag attatctgga
     2761 taccaatcag gcgtatattt accgcggata tcagcaaaat ctccagggct cgtccatgtt
     2821 cctagctcca caccatttag gctgaaagtg atatctgaag gccaattgtc atttgaaaaa
     2881 ggaaattcag aagaaatttc cacagaaact tccaacatct ccaatgtatc ctctgtattt
     2941 aaaaaattag gtgcctgata ctcgacaaat ccgctggtaa accagagaat gcgggcatcc
     3001 atccttcgag gatccataaa atagcgtggt tcatccactt tcccgataaa atcatggatc
     3061 gtagctaaac cgcatgttgg ttcaattgca taatttgtat agtgaccaat aggtatcgat
     3121 gtttctttcg tatcaaaagc attgaatatt ttttctggaa aactgatatc gattttatcg
     3181 acacgaaggc tcgaaatttt ttgttgtcct actttttctg ttttgatgat gtttgcttcc
     3241 tctagctttt tcacgtgcat cgtcgtgata gcactgctca ctccaagttc ttttgccaaa
     3301 tctttgacat tcatcttttt tttggaaagt agctggatga tcttgatcct tgttttacta
     3361 gctaatgctt catataccgg taaggattct tcgtttattt gtagttgcat atatatcacc
     3421 tcttaaaagg tttttcttat atattaacat atttgttaat aaaagttata ttaaattatt
     3481 tgttttaatc attattaata ttttggttaa ttaaaatcga taaaaaataa gtaagtttaa
     3541 catttatatt attgacaacg ctttcaacga ggtgtattat tcatgtataa attaaaggag
     3601 gtcagttatg gaagccacaa tgttgattag tagtgtcaag cgtgtaggca aagttgatcc
     3661 gcggttgtac agttcattta ttgaacatat gggacgagca gtatatgaag ggatttatca
     3721 gccagatcat ccatcagcta ataaaagtgg atttcgccaa gacgtcattc acttggtaaa
     3781 agaattgaac attcccatga ttagatatcc tggtggaaat tttgtgtcag gatttcgttg
     3841 ggaagacagt attgggccca tagcaaatag acctagaagg ttggatttag cttggcggac
     3901 aattgaaacg aatgaagttg ggattcatga attttataac tggtgtcagc atgtgggggc
     3961 cgaaatcaat atggctgtca atctgggtac tcgtggagta gatgcagctc gtaatttagt
     4021 cgaatattgt aattttccta aaggaactta ttggagtgat ttaaggcgtt cgaatggtca
     4081 agagagccct tttggaatta agacatggtg tttagggaat gaaatggacg gtccttggca
     4141 gattggacat aaaacagcgg aagaatatgg acggttagca gaggaaacgg ctaaggcgat
     4201 gaaactcgta gatgattcaa ttgaggtggt tttatgcgga agttcgaatc ggcaaatgcc
     4261 tacatttggt gattgggagt taacagttct tgatcaagcg tacgatcaaa tagattattt
     4321 gtctctgcat caatattatg gcaaccaaaa aaatgatctt tctcattatc ttgcacgttc
     4381 cttagacatg gatcggttca tttctgaagt gacagcgatg tgcgatacag tcaaggcgaa
     4441 gaaacatagt aagaagcaga tcaacctgtc ctttgatgaa tggaatattt ggtatcattc
     4501 agcggaacaa gatgagcaag cagcgccttg gcagattgct cctcctttac tagaagataa
     4561 ctataatttt gaagatgcat tactattagg ctgtctatta atcactttat tgaaacatgc
     4621 agatagagta aagattgctt gtcttgctca attggtaaat gttattgcac caatcaggac
     4681 agaaacaaac ggagaagctt ggagacagac gatctattat ccatttatgc aagctgctgt
     4741 gtatggacaa ggagaggtgt taagcccaca aattcgttca gcaaactatg cgacggaaga
     4801 ttttgaagaa gtgccgtatt tagaaagtat agccgtaata aatgacaaag aacttgtcat
     4861 atttgcagtt aaccgtggag aagaagaaat gaatctaact gttcacttgt ctgaattatc
     4921 attagaggga gtgattgatt tttctgaaat gagtggattt gatacaaaag caactaatct
     4981 tcctaaaagt gaacaagtaa aaccaagtca ttcaacaaat gtaaaagctg aagatgggaa
     5041 aatcaattgt ttattgaaac cgctatcttg gaatgtggtc agatgtcgtt tatcagatga
     5101 aggaggaaca aaaaatgaaa ggtaaaaaat ggttaattgg ctctttgttg gtagctgcaa
     5161 caggtatttt aggagcttgc ggtggcggaa gttcagcaga atctgattct gataaagaag
     5221 tgactttgac tttttggaat gggtttactg catctgatgg tgaaatattg caagaaatcg
     5281 ttaatgaatt taataaaaca aatgataaaa acatcaaaat tgaaatggat atcatgactt
     5341 gggccaattt gaatgaaaaa cttcctacag cgatttcttc taaaaaagca cctgacttta
     5401 ttgcattaaa ttatccagat tttgcacagt atgtagaaaa cggagcggta caaccattag
     5461 acgatttctg ggaatatgat ggagtagata aatctgattt taaagaaacg gctattgaat
     5521 taggtgaagt agaaggtaaa cagtattttg tccccatgca ggtacaaggc atgtatatgt
     5581 attggaataa tgatttgttt aaacaagctg gattagatac tgaacaacca cctactactt
     5641 gggaagaatt gacagaaatg gcaccaaaat taactgatag tagtaaaaat gtatcaggtt
     5701 ttgtcttcaa taaggatgga actgcaccgt tatataactg gatgttagca aatggcggta
     5761 aattagtgaa cgatgattat actaagtctg aatttgcgtc tcctgagaac cttaaaacgt
     5821 taaaagcgat ccaaaaaatg atccatgtag acaagactgg tccagaatcg atttctggcg
     5881 ccgaaatgga caatttaatg aatgctggtc aactagccat tgaaatcaat gggccatggt
     5941 taaataacgg attgaaagca aacgagattg attataatgt gactacctta ccacaaggga
     6001 gtgattcaga caaaaaaaca gctattttag atggcgtagg atatgccatt ccggcaagta
     6061 cagataataa taaaaaagaa gctgtctatg agtttttgaa atattggaat acaactgaaa
     6121 ttggtaaaaa atggagtatc gaaaatggtt ttcctgccta cttggattca gtagcaaaag
     6181 atgaagaagt aaaaaatgat cctattgtat ctgaattatc gaaacaaatg gactatgcag
     6241 agccatattt accaggtttt acccaaatct ctgcgatcaa taacgatatc atcaatccac
     6301 tgattgaaaa attattagca ggagaagatc ctgagacact catgaacgat gcagatgatg
     6361 ccatcaatca attactgcaa aaatagtaca gaactttata caggatagac tgttggcaag
     6421 atacagatat ctgaatgatc gacctagtat ttgcagatag gtgttattgc aaaaatcaca
     6481 gcgattgttc ggatatctta ttcagattcg tttaaaccaa gattgtatat tttatcaaaa
     6541 tgaaagatga ggtggactat atgaacggat tcaccaaata ttggaatcgt cctgatcgtg
     6601 ctgcgtattt gtttttaact ccatcttttt tagttttatt attatttact gtcataccgc
     6661 ttttaggaac atttgctatt agttttacag atttgaatat tttctttaca tcaagaaatt
     6721 ttgtaagttt tgaaaatttc acacgtatat ttagtgatga acgagcagtt aattcgttga
     6781 ttcattcttt atatttcacc gtactcgaaa cgccaacaca aattatcgtt ggtttattag
     6841 cagcagtagc attatctaaa aataccagat tcaatcgttt ttgtcgttcg acattttaca
     6901 ttccagttat ttgttcattg acgtcaattg cgattatttt ttctatgttg cttgatccga
     6961 atgtaggggc gattccttac ttgttttcac aagtcggctt gccgatccct caattttttc
     7021 gcgatccaac ccttgctatg ccgacggtag ccatcatgac tgtttggaag aattttggtg
     7081 ttactatgac gattctcttg actgctattc agggaatctc tccctctttg tatgaatctg
     7141 ctcaaatgga tggtgcaaca aatcgacagc agttttttaa cattacgcta cctcagatcg
     7201 ttccctcctt aggtttttgt attttaacga atctgattgg ttcgatgcaa gtgtttgacc
     7261 aagtatatgt tgcaaccgct ggtggcccac aatttaagac agaaacagct gttcaatata
     7321 tttacagtcg aggatttaca gcaccttatg aattgggcta tgcatcagca ctgtcttcgg
     7381 ttttgtttat cattgttgca actatagcta tctccactaa tgtgtacatg tcacggaaag
     7441 aaaaacaaat gaaataggag ggaaacctgt atggcaccag aaagctcgat tacagttact
     7501 gtgaatagtc gaaaagcagt tagtggtaaa aaaatcattc gaacgattag tatgctagtt
     7561 atattgttgg ttattgcgtt tccttttttg tggctgatta tttcttcttt taaacacgaa
     7621 aaagacatta tctcatttcc gcctagaata tttgcagata gctataccct agataactat
     7681 attaaggttt ggacaacgat ccctttatta gattacatca aaaatacagt gatttttgct
     7741 ggagggacag tgatcacttc tgtattcttt gattctcttg cggggtatgc atttgctcgt
     7801 atgcgtttca aaggaaaatc agttttgttc tatttcgttt tgctgacaat gatgatccca
     7861 ttccaagtct tcatgattcc tctatttatt gaagtcaatt tgcttggcat gcttgatact
     7921 tatgcaggtt tgatcattcc tcgtatgaca actgcatttg gtatcttcat gatgcgttcc
     7981 ttttttatta ctttaccaga ttctttggaa gaggcagcac gcattgatgg attaagtgag
     8041 tttaatattt ttttgaaaat catgttgcca ttgagcaaac ctactctttt atcattaggg
     8101 attttcacgc tgatgaatag ttggaatgac ttgctttatc cattgatatt gacaagtagt
     8161 tcaaaaatga gaactttacc agcagggtta gctttattta cagggcagaa catttctttt
     8221 tatggacctg taatggctgg aacggttatt tcaatgctgc cattactagt ggtttatatt
     8281 tttgcacaga aatattttgt tcaaggaaca gctatgtctg gaatgaaaga gtgaggaaga
     8341 tgaaaatagt tgagactaaa ctttatggac agttgattgt ggtttctgat tacatcttat
     8401 taggtattct ctgggtaatc gtgtgtttgc ctgttattac tatcgtgcct gctacatgtg
     8461 ctgtttttta tgtaatggcg caatggaaag gtgagggatc aggaaatatc atttattttt
     8521 tctttcaagg aataaagcaa cattttggta agaacttgtt ccttagtctg ttaacgatca
     8581 ttatttattt tcttgttaat caattattag aagaacagac aatcgtactt tcaattagtg
     8641 gatatgtagt tttacttatt tattccatgt ttcttttatc ctggatatat gtgatttctg
     8701 aaaacaaaga tatcacattt ttacaaattt ttgaacaaag tgcatggaaa gtattttttg
     8761 attttttgag aaatattctt tgttgcgtat tgttattagg atttgtactg ctcatatttc
     8821 ttttccctcc ttttattttt atctttgctg gtggcgtatg gaaacttgta tctattatct
     8881 tgactatagg aaatgaaagg tagggaaagc tgttggacga agaagtgaaa gtaaaaatca
     8941 gagaggattt tacggtagga gagatcagtt ctcggctgtt tggctcattt gttgagcata
     9001 tgggaagtgt cgtctataca gggatttatg aaccgggcca tgaaacagct gatggtgacg
     9061 ggtttagaca agatgtcctc tcttatgtaa aggaattaga attaggtgtc attcgctatc
     9121 ctggaggaaa ctttacatct ggttatgatt gggtggatac tgtaggtagg aaagaacaac
     9181 gaccaaaaaa aattgattta gcatggcgag gtatagaacc aaattctttt ggactccacg
     9241 aattttttaa atggatcagt catgtacaag cgaaaccgat attcacggtg aatttaggaa
     9301 caaaaggaat tgatgaagca aaaaatttag tagaatattg taattttcca aaaggaacct
     9361 tctggagtga tttaagaata aaaaatgggc aaaaaaagcc ttttggcgtt aaattatggt
     9421 gtttagggaa tgagctagac ggtccatggc aagtgggggc taagagtgct tatgaatatg
     9481 gtaggttagc aaatgaagcc tcaaaagtca tgaagttagt ggatgattca attgaaacaa
     9541 tcttagttgg aagctctacg ccacgtttac catcgtaccc tgaatgggat cgtatcgcat
     9601 tagaagaagc ttatgagaat gtagattatt tagctttgca taactatatt gataagtatg
     9661 atgaagaaga cttgactaaa cctcctaaaa atgagcgtga tgatacagcg acataccttg
     9721 caaaagctta tcgattcgat cgtcaaattg aagaaatcgt tgctacttgt gattatgtaa
     9781 aaggatattt gagaagtgag aaaacaatgt atctagcgtt tgatgaatgg aatgtacatg
     9841 cccaaccgga aaaaagacat agggattttg aagtcggtag tcccatcgat tggtgttact
     9901 ttactatgga agatacatta ttatttggtt cattagggct ggctatttta cgacacgcag
     9961 atcgcgtaaa gattagttgt caatcattgt tagtaaacac aataccttta atactgacag
    10021 atgctaaagg acaagcatgg aggaatccaa cttattatgt gatgcaacac ttatcgaaat
    10081 atgctaaagg tcaagtattg aaacaacaaa tcatttgccc tgtttatgat tgtgaaaaga
    10141 taaaaaatgt tccttatgtg gacagtgtga tcgtagataa cggcaatcag ctgactattt
    10201 tcttgatcaa tcgcacaaat gaacaacaaa agataacgat tgaacatgat ttcaatttgc
    10261 tagatcaagg agagcactac acattaacca gtagcgatct gttggacaaa aatacacgtg
    10321 aagaaccaga aaaaataaaa cctgaaataa actatttttc aatagaaaaa catgacgaat
    10381 taaatgtagg gatagaaaaa tactcttgga atgtaattag aataaagaaa atgtgaggaa
    10441 atttgttatg atgaagacac tttataataa tcctatcgtt cggcaacggg cagatccttg
    10501 ggtatataaa catacagatg gttattatta ttttacagga tcggttccag gctaccaagt
    10561 gattgaatta agaagggcaa agtcactgaa tgaattagaa catgctgcaa gcctgattgt
    10621 atggcaggct catgaagaag gaccgatgag tgaattgatc tgggcacctg aagtccatta
    10681 tatcaatgga aaatggtata tttactttgc tgcaagcaat cataaaacta tacgtgatga
    10741 atctcatcat caccgcatgt tcgtacttga aaatcagcat atagatccaa tgaatacaga
    10801 ttggatagaa aagggacaaa taaaaacgaa aagggaaact ttcagtttag acggaacaac
    10861 tttctcttct caaggaaagc tatattatgt gtgggcccaa caagatcctg aaattactgg
    10921 gaattctaat ctttatctat ccgaaatgga taatccttgg acgttaaagg ggaagcagat
    10981 cctcctttct atacctgagt atgaatggga aaaacggggt ttttcagtaa atgaaggtcc
    11041 agcagtcctt actcgcaatg ggaaaatatt cattacgtat tctggtagtg caacagatga
    11101 aaactatgcc atgggcctgc tgtgggctga tgagcacaag aatcttttat taaaagaatc
    11161 atggcataaa ttatcggagc cagtattcgt ttcttctaaa aaaaataagc aatttggtcc
    11221 tggtcacaac tcgtttacgg tttcagaaga cgggtgcttt gatattttga tctatcatgc
    11281 tcgaccagag aagaaccaaa aaggggatcc gttagacaat cctaatcggc acgcgaacgc
    11341 gcaatgcttt acgtggaatg agcaaggatt tccagaattt ggtgaacctg ttccgtatgc
    11401 cttatgagca caagaaacct tgatttttgc ttgaataaac cttgcagact cgttatgata
    11461 tatgtacgta caaataaaaa ggggcaaggc aaatggcaaa atatcaagat attgcagaag
    11521 agataaaaca aaagatcctt cgtgaggaat acgaagtaaa tacaaccatt ccctcagaat
    11581 tcaagctgca agaaatatat gacgtcagca gacatacgat tcggcaagca atcgctgtgt
    11641 tggtaaatga gggctatctg cgtaaagaaa aagggtcggg cacatatgta gatgacagct
    11701 ataagaaaga aaaaacacca ttgaacagaa caaaaacgat cggtgtcatc accacatatc
    11761 tctctgatta tatttttccg tcgattatcc gaggaatcga aaatgaatta cgagaatctg
    11821 ggtattcatt gcttttagcc agtacgaata acgatcacga attggagaaa gagtgtttga
    11881 agaaaatgat tgcttatggg gtagatggtt tgatcgttga gcctacgaaa agtaatcagt
    11941 ataacccaaa cttagcactt tatgttgctt tgagagaaca aggcattccg atggtgatga
    12001 tcaatgctgc ttatgaagag ttaaatgcct cttatatttg tttagatgat gtgaaagtcg
    12061 gttttatggc agcagaatat ttgataaaaa aaggacatca gcgtctgttg ttcatcacaa
    12121 aaatcgacga tcttcaagga aaatacagga tgaaaggttt tatcagcgcg tgtgaacagt
    12181 ataatgtgcg ttttttgacg gaagatatca ttactttcac cacggaaaca cgagaaacaa
    12241 tcgctgaaaa aacagtacaa cggttgaaag aagaacaagc tttagatggc atcatttgct
    12301 ataacgatca aatagctagt ttaatgctag ataagttgac agaaaatggc tatcatattc
    12361 cagatgacct atcgatcgta ggaacagacg attcttcttt aagccattta gggaataaaa
    12421 aattgacaac attgacgcat ccaaaagaaa agttgggaca agatgccgca cggtggatcg
    12481 tcacaacgat tgaaaaagga caggcacagc caaatattct ctatgaacct gtactagttg
    12541 aacgggaaac agtgaaagaa aacgaaaaat aagagacggg tttttaccgc ctcttattgc
    12601 tcctacggtt actcgtcgca atgtttgtga gagcttttgt catcgctcaa ggctgaacgt
    12661 ttctttcacc acctctccac taaacggtgt tctcctacca actggtcaca agtcacaata
    12721 ttcatcaaac atgagaagct gttttttgaa attgttcctt gtgactggaa actaagcggt
    12781 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttaagaa tgatggatag
    12841 aaatacgttt aggataagtt tttttagatg cactttgttc gatttcctct aaagatttcc
    12901 cacgagtttc tggtacgatc tcttgaataa ataaaacccc taaaaagcag atgatcccaa
    12961 aaatgccgaa aacgatttct tgagacatag aagcagtcat cacaggaaaa agcaagccaa
    13021 ctaggaaaga accaatccaa ttaaaagaag aggctaggcc agatgctcgc ccgcggacag
    13081 ctagtgggaa tatttcgcct acgattaccc atgtcagtgg agcccatgtg caagaataaa
    13141 atgcgacata aatacataaa aacaagagaa tcaacaacga atttgtatga gcatccagta
    13201 cagttcctaa aacagctggt aggatgaaag aaagtcccat cacgctaccg cctattttca
    13261 ataaagttcg ccgattgaac ttgtctgcaa taaccaaaaa gagcaaagaa ccagcaacta
    13321 aaatgattcc ttggatgatc ggccacatca atgcatcgct cgctgcatgc ccagtcgctt
    13381 tttctacgat cagcggaata tagtaaaaaa tagcattagc tccttgaaat tgctggaata
    13441 ctgctacacc aataccggca atgactaagg aacgatattt ttctaaaaac aaagtatgcc
    13501 aagaagttcc ggttgtcttt tcttgtttga cattcagctg gatttgttcg aattcctgtt
    13561 cagcttcttt tggtgtccga atatagttca atactctttt tgcctcttcc aagcggccag
    13621 attgcataag aaaacgcggt gactctggta gagctaaaac accgaaaaat aaaatcagcg
    13681 cggggacagc agcgagccct aacatcacgc gccaagccat cgtttcaggc aaccctttta
    13741 gcaaataatc tgcaacatag gaaaggagca tgccagaagc aatcatcact tgattgatcc
    13801 ctgacaatct tccccttagt ctagcaggag ccatttcaga catataagca ggaactaggg
    13861 cagatgcagc gccaacagct aaacctagaa gaatccttgc tgcaatcaga aaataagaac
    13921 cattgtgagg ggcagatgca gaaaggacag aaccagccat aaaaataagt gcagaaagaa
    13981 gaatcatttt tcgtcgtcca agcttatcag aaagaagacc agataaagag ccgccaagga
    14041 tcgcacctaa catgaccgaa gacgtgatcc atcctatcaa agaagcttta cctgccaaac
    14101 cccaatcatg ctgtaaaaaa ggtaaggcgc cggtcataac accaatatca tatccaaata
    14161 gaatgccgcc aaatgcgcca aaaaaataaa taaacgatga ggaaactttt ttttcttttt
    14221 tcataagtat tctctccttc tagaatttcg agaaggcggt tgatcagatt ttgtaagcaa
    14281 tttcatttta taacttatgt acgtacaaaa caagtgtttt ttattgtatt cttgtaaata
    14341 actgcttgac atgtacgtac aaaagtatac aataatcatt gtaagcgcta ctaaaaagac
    14401 ggtttgatca ggtttgacga gaaggaggga gttaaatcat tatgaaggaa gtaaatgcgc
    14461 aaagtatgtt agatgtcata gatggtcgta catctttagg gattgagttt ggatcaacta
    14521 ggatcaaagc tgtattgatt gattcatctt ttactcctat tgccgctggt agttatgagt
    14581 gggaaaacaa actagaaaat ggtatctgga cctattcatt agaagatatt tggaaaggac
    14641 tgcgcgtcag ttaccgaaaa atggcagcag aagtattcga aatctatggc gagaatctta
    14701 cgacaatcgg gtcgattggg ttcagtgcaa tgatgcacgg ttatctagca tttgatagca
    14761 aggaacagtt actagtacca tttcgtactt ggcgaaatac aatcactgaa gaagcagaac
    14821 gaatattaac ggaaaaattt caatacaata ttccgcagag atggagtatc gctcatttgt
    14881 atcaatctat tttgaataaa gaagaacatg tgaaagaaat taattttttg acaacgcttg
    14941 caggttatgt ccattggcag cttactggtg agaaagtact aggcgtagga gatgcatcag
    15001 gcatgtttcc tatcgatcct tcaacgaaaa cctaccatca agaaatgaaa aatatttttg
    15061 gtcagttaat cgaaaaagcg cacgtagcga ttgacttgga tcacattttg cctaaagttc
    15121 tagtagcagg agaagaagct ggcaagttaa gcgaatcagg ggcttatctg atcgacccta
    15181 cgggaaattt gagaccagga atcccggttt gtccgccaga aggggatgca ggaaccggta
    15241 tggtcgctac gaacagtgta gccaaaggga cagggaacat ttcggctggt acttccgcct
    15301 tcgctatgat cgtattagaa aaagaactag aaaatgtcca tccggaaatc gacatggtca
    15361 ctacaccttc aggagaacta gtaggcatgg ttcatacgaa taactgctct tcggatatca
    15421 atgcttgggt cagtattttt gaagaattta ccaattcttt gggtatggag ataagcagag
    15481 ataaattatt cacggtatta ttcaacaaag cgctagaagg cgatacggat tgcgggggat
    15541 tactttctta tggttatttt tccggtgaaa acattacagg agtaaatgaa ggaaggccat
    15601 tatttgtacg tacaccaggt agccgttttg atttagcgaa tttcatgcgg atccatttag
    15661 ccagtgcttt tggtgcaatg agaatcggaa tggatatatt gaaatctgag aatgtccaaa
    15721 ttgatcgttt agtggggcat ggggggattt ttaaaacacc agaagttgga cagcggattc
    15781 tagcatctgc tatggaggca ccagttacag tgatggacac agccggagaa ggtggagcgt
    15841 ggggaatcgc gttattagca gcctatatga tggatcgaca tgggaagtct ttagaaaact
    15901 tcttggcaga agacgtgttt ggacaagacc aaggaatcac catcacacct tcagcagaag
    15961 aaatagcagg ctatcagata tttatgagac gttaccgtga ggggatcgac attgaaaaaa
    16021 cagcgattca tcaattgcaa gaaaaaggag tgatagaaaa tgctggaaca actgaaagaa
    16081 gaagtatacc aagcaaatct tgacttgcct aaacacggac tcgtcaaata tacatgggga
    16141 aatgtgagcg cttttgatcc tgaaacacga tatttcgtta tcaaacctag cggagtcagt
    16201 tatgaagaga tgacagcaga cgacatggtc gttgtggact tggacaatca tatcattgaa
    16261 ggtaaactca atccctcttc ggatacgcct actcatgctg ttttatatcg ttcatttcct
    16321 caaatcggag gaatcgtcca tactcactcg acatgggcga cgatctgggc acaagcagga
    16381 ttggatgttc ctgcgatggg gacgacgcat gcagatactt tttatggatc ggtaccttgt
    16441 gctcgatttt tgacacaaca ggaaatcgac agcgggtacg aatatgaaac aggaaaagtt
    16501 atcatagaaa cattcaaaga acgcaaaatt gatcccttag caattccagg ggtactttta
    16561 cacggacacg gtccatttac ttgggggaaa gatgcgcaaa gtgcggtaat gaacgcagtc
    16621 gtactagatg aagtatgcaa gatgaattta tttaccagac agctcaattc attttcagaa
    16681 gagctgcctc aacggatttt agataaacat tatttacgaa aacacggaaa aaatgcgtat
    16741 tacggacaaa acaaataaaa ggagtggcta attatgttga atattggaga aaaagaattt
    16801 tggtttgttg taggatcaca gcatttatat ggggaagaag cactgcgaga agtaaagaaa
    16861 catgcacaag aaatggtcga tgaactgaat gaaaatggcc aattgcctta tccaatccgc
    16921 cttcaagaat tagcagttac tgctgatacg atcacaaaaa tcatgaaaga ggtcaattat
    16981 cgagaagaag tagcaggtgt gatcacttgg atgcatactt tctcaccagc aaaaatgtgg
    17041 atccgcggga caaagcttct acaaaaacca ttgcttcatt tagctacgca atataacgaa
    17101 agcattcctt ggaaaacgat cgatatggat ttcatgaatt taaatcaatc cgctcatggt
    17161 gaccgcgaat atgggttcat caatgctcga ttaaataaac aaaataaaat cgtcgttggc
    17221 tactggaaac gacctgagat ccaaaaagag attgctgatt ggatggatgt tgcagtagcc
    17281 tataatgaaa gttttggaat aaaagtggct cgttttggtg acaacatgag aaacgtgggt
    17341 gtcacagaag gagacaaagt ggaagcacag attcaatttg gttggactgt tgattatttt
    17401 ggtatcggag atcttgtaca agtaatcgat cgcgtatcag acgaagaagt cgatcaatta
    17461 tttgaagagt acaaggaatt gtataccttc gactatggtg attatgaaga aaagacatgg
    17521 gaggaacatg taaaagtaca agctcagcag gaaatcggta ttcgccgttt cttagaagaa
    17581 ggaggctata acgcttttac gactaatttt gaagacctgt atggaatgaa acaactacct
    17641 ggactagctg tacaacgttt gatggcagaa ggatacggtt ttgctgggga aggcgactgg
    17701 aaaactgcag cgattgatcg gttgctgaaa atcatggctc gagggaaaga tacaggattc
    17761 atggaagatt atacttacga gctagctagt ggccaagaag caatcttgga atcccatatg
    17821 atggaagtag atccgacgct tgctgcgacg aaaccaagaa tcgttgtttc tcctttatca
    17881 atgggagata gagaagatcc ggcgcgtctt gtatttgatg ggaaagcagg agaaggggta
    17941 gtcgtatcga tggcagattt cggtacgcat tataaactgc tgatcaatga agtagaagcc
    18001 tttgaaccaa cgactgaagc acctaattta cctgttgcac gtgtgttatg gaaaactaaa
    18061 ccgaatttcc atgaaggtgt ccattcatgg attcaggcag gtggcggtca tcataccgtt
    18121 gtctcgctaa atctaacaac agatcaaatt gaaacgtggg caaaattagt tgagttggaa
    18181 acagtggtta ttagataaaa agaggttgtg aaaaagccac ttagttttaa gaaataagaa
    18241 gagattgacg aaatttgctt ttcaactttt ttgaaattta gtcttattat caatgtcagc
    18301 taacggaaca ccatttatca agatatctca ccctcaatat tttgctctcg aaaataaggc
    18361 ttatggcagt aacttgtcat agccttattt ttatcactca gcctactctg ctctaactgc
    18421 tctttttgct tctaggaagt caaatggtaa acagttgtct aattgcagaa atttcgagat
    18481 acttgttagt aaggaggggg ttttatgtat cacgttcatt tgattgatgc tattccagaa
    18541 caagagatag aaaagttaca aggggaatat ttcaaaataa tagatgattt gccagccgaa
    18601 ggaatagtta ttcgtagtac gaaagtgaaa gatgaatggc ttacaccaga tttgctagca
    18661 atttcaagag ctggtgttgg ggtgaacacg atcaacgttg aaaaagcttc tgaaaacggg
    18721 acgatcgtca tgaatacacc aggtgtaaat gctaatgcag taaaagagct tgttttgtgc
    18781 tgtttgctct tatcttcccg tccgataatc gaagcaagtc gtatggtaca aacactaaca
    18841 ggtcctaata ttttagaaca ggcagaaaac aaaagaagtg cttatgtggg acgagaattg
    18901 caaggcaaaa ccatcggtct tcttggtctt ggagcgatag gcacaaaagt agcgctaagt
    18961 tgctatagct tagggatgga tgtcttgggt tattctatcc gcgatgccca gttggattat
    19021 gttcggcaag cagatttaga aacagttctt tctacctcgg attacatcgt agtcatgctg
    19081 ccgttgacag aagatacaaa aggattgatc gatcaggcga atatagaaaa aatgaaaaaa
    19141 gatgctgtac ttatcaatgt aggaagaagt gaaattgttg ataaatatgc tgttatgcaa
    19201 gcattggaaa aacaacatct ggctaaatac ttgactgatt ttccagaaga agagttctta
    19261 gaaaatgacc gtatacttat gttgcctcat ctagggggat cgactcagga agctttggca
    19321 gacagcggac gtttggcagt tgaagcctta aaagattatc ttttgttcgg tacggttaga
    19381 gaagcggtca attatccatc cgcacgaatg tttttccaag cgccgtttcg attcactata
    19441 ttttatcaaa agaaaaccaa tatattagca gagatatttt ctctgctgaa tgagaacgag
    19501 ctagcgatcg cagatatatc gcgtaaccat aagaatgagc atgtctacat attgattgac
    19561 atagacagta ctgattttga acagctcaat caagtgaaac aacagctaga aaaaatttct
    19621 ggagtacgaa aggtccgctt attaaagaaa ccagaaaggc cataaagaaa gagaatattt
    19681 ggatttcatt aattcttctt tccgttcttt actttcgaat ttcgtacaac taaaataaga
    19741 gaagcaacat tcttttattg atagaaatga aggagcaagc atttgaaaag atatataagc
    19801 agactttcga ccaaacagaa aaagatttta aaaggaacag cagcaatcat cagtatcttt
    19861 tttcttgttg tttttagtaa tttatttttg cagtggtgtc aaaatgatct atccgtagac
    19921 cttgcgttga aatttgcttt ttcttggcat acggaaaaat tcttcttagc ctgtctcgtc
    19981 ttgctgatta ttcttatttt tctgattgca ctagcaggat ctgtcccttt aggttccctt
    20041 acgtatgtcg tagccatcgg tgtattaggt tttgcaaatt atatgaaaat gagttatcgg
    20101 caagaaccga tttatccaga cgatttgaag atgatcactg aaatcggatt gctgaaagat
    20161 atgaccggca ctatgctgtt tacagtgatc ttagctgccg caggaaccgt gataggattg
    20221 ttttgctggt acatgtttcg aagcctgaaa aaaggaaggc gattccaatt gatccgatta
    20281 acgacactgc ttgtggcaat tggactgcta gggtatatca gtaatttcaa caatcccgat
    20341 aatttgttgc gaaaagcata taataaaacg gcactatgga taccttacag ccagaaaatg
    20401 aactactata acactggttt tatcggtggg tttttgtaca atctgaaggt agaaccaatg
    20461 gatgaacctg aaggatattc gaaagaaaag atcaaagaaa tcactgaaaa ataccagaag
    20521 ctagccgatg aaaaaaataa aactgtagag gaagagtcgc cgaacatcgt gttcgtgatg
    20581 agtgaaagtt tctcagatcc tagtcgatta aacggcgtag aagtatctgg tgagccattg
    20641 gcagattatt atgaagtagc tgatcaaaca tatagtggca atatgctttc tcaaaactat
    20701 ggcggtggaa cagcaaatat cgaatttgaa gcattgactg gtttttctat ggcgttattt
    20761 aatgcgcaac tcaccacccc ttacacgatg cttgtaccaa aaatggatca gcttccttct
    20821 atcgtttcag cgttaaacgc acaatcatac cagacgacag cgatccatcc atacaatact
    20881 tcgatgtata aaagagagga tgtttatcag acattaggat ttgaccagtt catcagcgag
    20941 cgtacgatga cctatacgga tacgatagag aataatccat atatttctga tgagtcagct
    21001 tacaaagaag taatgacact tctcaaggaa gaaaaaacac ctcagttcat tcatttagtc
    21061 acgatgcaga cacatatgcc ctacgatgga aagtatgata atctagacta tcctgcaaaa
    21121 acttcagatg gaagtgggac acttgactta gagaattatc tgcaagatat ttcctatagc
    21181 agcacggcac tgaagcagtt cacggaagaa ttgaagaatc tttctagaag gactttagtt
    21241 gttttttggg gggatcatct tccagggatc tattcagata cgatccaagc aaaaaatgac
    21301 aaacaaacat tgcatgaaac acaatttctt atgttcgaca gtaaaggaga attagaaaag
    21361 caaacgactc acgatgcaat caccagtccg ttttattttg cagcgaatct aatggaacag
    21421 acgaatcaga caacaaatgg cttttatcag ctgcttttat cattagaaca ggaacttccg
    21481 gcgtttgaac gagaactgta ctatcaaaac ggacagtggt ataaagaagc gcaattcaat
    21541 cgctcgcaac aagagattta cgacgaatat caattgatcc aatatgacat tgtcgcaggg
    21601 aaacaatata gtttagcaga tggtttcttt gagcacgaat aagaatggcg agctgtatag
    21661 aaaccattaa attttttcaa gtacttgtca tttgaggata aaaacaagta gaataaataa
    21721 taacaacaat ggagaaagta ggaaaacgaa catgttaccg aatattggct attatgtgca
    21781 gtccatcgat caaatcgtca aggaaacaga agcaatcgga gaaggaatgc atccgaaata
    21841 tgaagtgatc agaaacgcaa ttgatgaagg aaaaacaagt gaactggaca aatcgactct
    21901 tgaagagatc atcctgttat ttgaagaagg aacaaaaaaa tatcgggcaa tgcttatgaa
    21961 agtcagtact ttgcgtccac cagcaaaagc aatgggcatt cataaaaatt ttgaaaaagc
    22021 ttatctttca tatgtagcag gctgtgaaga aatgatccaa agtatcgatc ttgaatcagg
    22081 ggtagatgaa gatctattca atacatctga agcaaaacaa gaccaagcga cagaccaatt
    22141 gacagctgcc atccaaaaaa tgactaattt gttgttgaaa aaataaaaga aatctttgat
    22201 aacaataaaa actagagtta tctaatataa atgtaatatt acctttatga tagagtgatc
    22261 aaaatccttt atgataatga catacctata accaacaaca accttgtact tactcaaaat
    22321 cgagtaaata ctccttttcc gtcgagtcta accatctcgg cggtttttta tttgtagaaa
    22381 agaacatcag gatagacgga aaagtcttgt cctgatgttc ttttcagata gtatggaaag
    22441 ctgattagtt ttctacattc tgtacatatt gttcaaagta gttctgtaaa tcggctttga
    22501 gtttctgata ttcctcttcg ctatactctt tatcttcgat tttcccatca aagaaaggaa
    22561 tatttagttc ttcccgtagt ctgtctaata cgtcttgctg attcatttca ttctcctttt
    22621 cttactaacg atcaaaataa acacagacac attctggtac atagacatct tttttcagta
    22681 attccagtaa tcctgtttca ggatgacggc gatataatgt cagattatcg gtgttctgat
    22741 tggcacacac gacaaaatta ttgcttggat ctagtgcgaa atcgcgggga aaatcccctt
    22801 ctgttgagat gatttgcttg ttttgaatgt gggaaccatc ttcaaaaact gcgaatacag
    22861 caatcgagtt atgtcctcgg ttagaagcat aaaggaactt gccatcattt gaaatgcgga
    22921 tagctgcccc tccattttca ccgtcgaatt cttctggcaa ggtcgatatt ttctgtaatt
    22981 cttcaaaagc acctgtttct ttattataag aaagcacgcg gactgtgctg tctaattcac
    23041 caaataaata agcgaattgt ccatttggat gaaagacaag atggcgaggg cctgttcctg
    23101 gctctgcagt gaaggtagtt acaagattca atttgccgtt ttcggacaca tcataagtat
    23161 acacacgatc tgtaccaaga tcacatacga ctaatcgttg gtcaggagtc agatccgtat
    23221 agtggacatg cgcatgatct tggtttttat gaggacctgt cgcttcttga tggaatattg
    23281 aatctgctgc tttcagttcg ccatctccta atatatggta gacatttact tcacctttat
    23341 gatagttcgc cccgtatagt aattgacgtg cttcatcaac cgctacataa cacaaaggag
    23401 cgccagactc tgttaccgca ttcaaaaaat gaaaatcctt atcgtacgca ccggcaccac
    23461 ctaatccatc cacactagta actgtgtaaa gattgccagc tttacttttt gctacatagg
    23521 tagggctatt ttcttcaaca gctaatgaaa ggttggctaa ttcttctttt tctgtatcaa
    23581 ggtcgatcgt atagattcct ttactttcgc gacgcgtata ggttcctaaa attatctttt
    23641 cgatcatatg agctgattcc tcctaattgt aatgaaatag ttttcgtttt tattctatca
    23701 cataattatt ccacacggct aaagaatgag aatgtgttat actgtttttg aaagcaatta
    23761 aaggagagag tgcaatgatt caggcaaaga ttacagaaat tgggacagaa gcaataaaca
    23821 aggaagaacc catgctgatt ttgttcgata aaacagcaac agctgcctta cgaaaatatt
    23881 cggtgattca agaagtatca aaagacgaac ctttttcttt gaaagcaggc ggaacaattg
    23941 cttttgatga tcaggtatat acgatcgatt atgtcggtcc gatggccaat gggaatttaa
    24001 agacagtagc tcacgtatca ttgatttttg atgcttgtcc agaagaagga caaattgcaa
    24061 atggaatcta tcttcatccg catagactgc ctgctattgg tttgggaagc caaatttttt
    24121 ataactaaca ggaggaatta tggaaggaaa aaataagaag aacagaccgg tatcgtggtt
    24181 ttggcgctgg tttttaaata atcaagtagt cacagcctta cttgttgtat tgttaatatt
    24241 gctgatcgtc tttttattta caaaagtttc atatttgttt gcaccaatct ggcaattttt
    24301 agcaatcgta ggtctgccta tcattttagc aggaatcttg tattacttga tgaatccggt
    24361 agttgattac tttgaaaagc gaaaagtacc aaggctttac agtatcattg ggctgtttgt
    24421 tttggttgtt gcactaatcg catgggggag cgtagtgatc atccctaaga ttcaggaaca
    24481 aacagtgagc ttcatcagta acttccctca atatgtagat acgattgata ataaattaac
    24541 cgagattttg cgggacccgc tgttcaacca atttcgcgaa cagttggaaa cagcaggtga
    24601 taagttcatg aattcggcag gcgatatgat ccaagatata tctaagagta cagttcagag
    24661 tcttggaagt tttgtcggcg cggtagcgac tattttagtg gctctgctga cgatgccgtt
    24721 tatcttattt tatctattga aagacggtaa acaattagca ccttattttg tcaaattttt
    24781 accgacacgc atgcaaaaac cgacgttgaa tgtgctgagt gaaatgaata gtcaagtttc
    24841 ttcttatata agaggacaac taactgtggc ttttgctgtg gcaatcatgt ttatgattgg
    24901 ttttgcggtc attggtttag agtatgctgt cacgttgggg attattgcgg gttttttaaa
    24961 cctgatccct tacttaggat catttttagc gatgattccg gctatctttt tagccattgt
    25021 agcaggtcca ttcatgatca tcaaagtact gatcgttttt gcagtagagc agacaataga
    25081 aggacgcttg atctctccat tagtactagg gaatcagtta gcaatccatc cagtaacgat
    25141 tttattagtt ctgctgactt ctgggaaatt gttcggaatc atcggtgtga tcttggggat
    25201 tcctgtttat gcagcagcta aagtagtgat tactcatgtt tttgaatggt acacaacaat
    25261 ctcttctctt tatgaagaaa atccgttgaa aaagcagaaa gaataacaaa aagagacaga
    25321 tgggaaaata tccaaggcac aaatgctgct ttgaagattt tttcctctgt ctcttttgta
    25381 tgcatttatc tcaattttta aaatgattta cctaaagcag cagctttatc taatgcattt
    25441 tgcatcaact catctgcacg gtcagggtca taatcgattc cttcgatgaa tagctgatct
    25501 acttgatcga tgcctacaaa gtttaaaata ccttttacat attgagaggc aaagtcttgt
    25561 ccgttgtata caccgccatt agattggatg tgcagtgctt ttttacctgt cactaatcct
    25621 tctggcccgt tttctgtata gcggaacgtt tttccagcta cattgacggt atcgaaccaa
    25681 gcttttaatt ttgttgggat atttagattc cataaggcgt ttgcgataac gattttatca
    25741 gcagcaagga actgatcagt caattcattg aaacgagcaa ttttttgttg ttgactttct
    25801 gataaggaag tgaattctgc accagcacgt aatgcttccc aacctgataa taattcttca
    25861 tcgatttccg gaatatcatc gcgataaagt tccaaaacag tcacttcatc atctggatga
    25921 ttttctttat aacttgttaa aaaagacgtc aatgccttaa ctgtgcgcga ttcttctgca
    25981 gttaggggat gacctttgac tactaaaaca gatgccatat gcttaccttc tccttttatt
    26041 gctatgattg ttataactat gactaacaaa atcagtttac tcgaaaaaga aaaggctatc
    26101 aagtcatatt gataaggttg aagaataaat aactaaaaaa agacagggta taaaagaggc
    26161 tgtgacacga ttttttgtca cagcctcttt tgaatctaac aagcaatatt caagcaacac
    26221 tcgactgaag agcttccatc ccaccaatga tataaacagc acgaaaacca ttagcagtaa
    26281 gttgatcggc taatgcttct gatcgcctgc ctgtatagct tagaatataa taaagtttct
    26341 ttggatctag attttttaat tgtcttggca agatagcagc ggggatagaa agcgagtggc
    26401 ttaagtggtt ttttgtgtaa tctaaggtat ttcttgtatc taaaacgatg ctgtcagcat
    26461 cgagaggcaa tttattgaag tcttggacac taattgattg ggccatttat ctcatccttt
    26521 gtttagtatg tggctatttt aatgggcaac tctcattaat tcaaatggaa ggattcctct
    26581 tatcactttt ttaatgtatc ggatgataat gaaaaaaact atgcaaatgt cttgcaaaac
    26641 aggtaggagg tgttataatg acatagcgtc atatgacaat gtgtcatctt gaaagaaagg
    26701 agaatccata tgccgacaca gacatttttc catttgccta aagaaaaaca aaaaagacta
    26761 atcgaggctg cgagaatcga gttttctaga gtacccttaa aagaagcttc gattgctaat
    26821 atcgtcaaat tagcggatat ccctagaggc agtttctatc agtattttga agataaagag
    26881 gatctttatt tttattactt tgagacaatg agacgtgaca gctctaaaga tatgttacaa
    26941 ctaatgaaag aatccgatgg tgatctattc aaaggattcg aatcctattt ttctaaaatg
    27001 atttatgaga ttttggaagg cgagaatgct tctttttatc gtaacttgtt tatgaacatg
    27061 gattaccgtg cctcaagaaa agtcgctcct catacgagtc gccaaaaaac gcctcacgat
    27121 ccacataaag aacacaaaca agatgcaaaa gagttgcttg aattgatcga tcggacgaag
    27181 ctgaaagtaa aaaatgatca tgaattggaa ttactgatcc aattagtgat gaatactgta
    27241 tttacaacaa ttgcgcattc gtatcgctta ttttcaacat ctgaagatta ctcagtagag
    27301 caagcaatca gtgaattcaa tttgaaactt tcttggctga aaaacggagt atataaatag
    27361 aaagaaggga actagatgct aaaaatcgta aaacggatct ccttgacttc agcaattgca
    27421 gctgcagttt tcatggtgat ccaagtactg gcagatttat atttgccgac acttacctcc
    27481 aatattattg ataaaggggt agcgcaagga gatgtcgatt atatttggca tacaggtttt
    27541 gtgatgatcg gcttttcttt gatcagtatc ttagctgcta tcgcaaatac attttttgct
    27601 actcgtgaat cacagaaact gggtaagcaa ttgcgtacgg atgtctataa aaaatcagag
    27661 agtctgacaa aagatgcgtt tgataaatat ggaacggcct ctttgatcac aagaacgacc
    27721 aatgatgtga ctcaaatcca aatggttaca caaatgtttt tacgtatgat gatcaatgca
    27781 ccaatcacgt tgattggcgc gagcatactt gcttatcaaa aagatcatca gttgacaaaa
    27841 atcttcttag ttgtcatccc agtaatgatt attttgatcg gcggaatcat gtactttgct
    27901 gttccattat tcaaaagtat gcaaaagaaa actgaccgtt taaacctggt atttcgagaa
    27961 ggattgacag gggtcagagt gattcgagca ttcgataaga cacgctttga agaaaatcgt
    28021 tttgatttag cgaataaaga ttatacaaat acggcaatca aagttaatac gatcgtcgca
    28081 ttgatgatgc caatgatgac attgatcatg agcggtacaa acgtagcaat cacttggttt
    28141 ggtggacact acatagcaga tatgactttg gaagtcggta atctgattgc atttatgact
    28201 tatgcgatgc agatcttgat cagctttatg atgctttccg ctatttttat catggtgcct
    28261 cgtgcacaag cttcagctga ccgtattaat gaagtgttgg atgaaaaaat cggtatccat
    28321 gaccctgaaa atcctaaaac agtttctttt gctggaaaaa acgcaacatt ggccttcaat
    28381 catgtcaatt atcgatatca tggagcagaa aaacttgcat tggaagatat tgatttccaa
    28441 gccaagtcgg gcgagatcgt cgcaatcata ggggggactg gttcaggtaa aacgacctta
    28501 gtcaatttga tccctcgatt atatgacatc gaatctggtt cgatccaaat aaatggaacg
    28561 gacattagtg acatgactca atataatttg cgagaattaa tgggctttgt tcctcaaaaa
    28621 gcagttctgt tctctggaac gatccgagac aatatgcagt atggtaagcc ggatgcaaca
    28681 gatgaaatga tttggaaagc attagaaatc gcacaagcta aagattttgt ttcagagatg
    28741 gaagatggct tagatagcca tgtggaacaa ggaggaggaa acttctctgg tggacaaaga
    28801 cagcgtctgg caattgcacg ggcattagtc aaaacagcag atatctatgt gtttgatgat
    28861 tccttctcag cgttggattt caaaacggat gccaatcttc gccaagcatt gaaaacgaat
    28921 atgaaagaaa gtatcaccgt tttagttgct caacgagtaa gtacagtaat ggatgcagat
    28981 atgatccttg tgctagatga aggaaaactg gttggcaaag gaacacatga agaactatta
    29041 gcaacaaacg aaacttatca agaaatcgtt cattcacaac taagagagga ggacttagca
    29101 tgagtaaaac aaacacacgt ccctctcatg gaccagcagg cggtccagga catggtggac
    29161 ctggcagaaa tctcggtgta aaaggtgaaa agcctaaaaa tttctgggga acggtacgcc
    29221 gattgtttgg ctatatgtct aaacgtatgg ttgcgatcat cgctgtattt gtactagcaa
    29281 tagcagccgt gatcttccaa atccaaacac caaaggtctt ggggaaagca accacagaaa
    29341 tattcaaagg tgtaatggct ggcgctcaac agatgcaaca aggccaacaa gtaacaaaat
    29401 ttccgataga ctttgataaa atcgcgcaga tcattgcgac agtaatcgtg atgtatttga
    29461 tttctgctgt attcaacttt ttacagcaat ttattatgac acgtgtttcc caacgaacag
    29521 tttatgaatt acgtcatgat ttggaagcca aaatgaatcg tgtaccgatt tcctattatg
    29581 atacacatac aaatggggat atcatgtctc gagcaatcaa tgatatggat aacatcgcaa
    29641 gcacgctgca gcaaaatttg actcaatttg taactagtgc agtcacattg attggggtca
    29701 tcgtgatgat gctttcaatc agctggcaat tgacgttagt cgcgttattg atgatccctt
    29761 taagcttgat catcgtgatg atcgtagcgc cgaaatcaca agtgtttttc gcagatcagc
    29821 aaaaaagttt aggtttattg aataaccaag tagaagaaac atatggtggt cataccattg
    29881 ttaaaacatt caatcatact gaaaaagacc aggaagtatt tgaaaaagaa aatcagaatc
    29941 tgtatgccgc tggttggaaa gctcaattta tttcggcaat catcatgccg ttgatgaatt
    30001 tcgttaaaaa tctaggctat gtgttcgtcg ctgtactagg cggagtaaaa gttgcaaatg
    30061 ggaacatgac attaggggat gttcaagcgt tcttgcaata tacaacacag ttctctcaac
    30121 caatcactca acttgcaagt ttgatgaaca ctatccaatc aacagttgct tcagccgaac
    30181 gggtatttga agtgttggat gaagaagaga tgtccaatga aaaatctggc cttccagaag
    30241 aaaaagatac accatacaaa gttcgttttg aaaatgttca attcggctac acaccagata
    30301 acttgctaat gactgatttt aacttggatg tcaaacctgg tgagatggtc gctattgtag
    30361 gtccgactgg tgcagggaag acgacactga tcaaccttct tgaacggttc tacgatgttt
    30421 ctggaggtag tattcgttac gatggtgtag atacccgtga tctttcacgt gatgaattgc
    30481 gcgcacagtt ttcaatggta ctgcaagata cttggctgtt tacaggaagt atctatgaca
    30541 atatcaaata tgggaacgac gatgcaacag acgaacaaat cattgaggct gctaaagctg
    30601 ctcacgtaga cgattttgtt cgtaaattgc cagaaggata taacactgta ttaaacgagg
    30661 atgcaagcaa tatttcgcaa gggcagcgtc agctaatcac aattgcgcgg gcattcttag
    30721 caaatccaga tgtcctgatc cttgatgaag caacatctag tgtagataca cgaacagaaa
    30781 tactgattca aaaagcgatg aaccgcctat tggaaaaccg gacaagtttc gttgttgctc
    30841 accgcttgtc tacaatacgc gatgcagaca acattattgt gatgaatcat ggttcgatcg
    30901 tagaaacagg aaaccacgat acattgatgg aaaaagacgg attctatgca gatctttata
    30961 acagtcagtt ttctgaagaa gaagcttcat agaatagtac aaatttatta ttttttttag
    31021 gggctgtgac aaaagtcgtg tcacagtccc tttttccgaa taaacggtgt tcaatcagct
    31081 gtccttcggt attagctcaa aaaacggcaa aatatgagaa gctatttttc cgttttgtat
    31141 ctctaatact caggtcaaac cgctgatttt acaacctctt ccttaaacgg tgttctcctg
    31201 ccaactcctc ggtcacaagt cacaatagtc aacaaacctg agaagctgtt ttttgaaatt
    31261 gctccttgtg actcggagtt aaacggcagg ctcacaacct cttggtaaac ggtgttcaaa
    31321 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
    31381 atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    31441 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg
    31501 tttggctgtt cttattactt gatgcagaac agctttttca caacctcttt ttataagagt
    31561 ttttactcat taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac
    31621 aaaatcaaag ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag
    31681 aggggagcaa gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta
    31741 gccattcggg tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt
    31801 gatttgaaaa gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa
    31861 atgttgctgc tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg
    31921 aaccgttttt cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc
    31981 cgtaaaatga attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg
    32041 ctcgtagacc cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa
    32101 agagaaattc cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc
    32161 agtaaactat caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca
    32221 aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc
    32281 atccatgtat tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca
    32341 attgatttgc ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact
    32401 gaaagcatgg aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac
    32461 aaaaagcaca cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt
    32521 gagccaaaag aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa
    32581 cgattgtcgc atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag
    32641 aaagagaaaa tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa
    32701 aaaaggttga ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa
    32761 gcaggagaaa aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg
    32821 attttatcca ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc
    32881 cggctttgcc caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc
    32941 agcctcactg acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg
    33001 aaagatagaa ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat
    33061 cactttgcgt catttactga cacatacttc tgatatcaaa acatggatac caaacagaga
    33121 taagctatct caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact
    33181 aggaaaagaa gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga
    33241 aatttaccaa gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat
    33301 gatgaacagc ttatttcttc cttttccttc caaaaaaatc gttcccacag aacaactgcc
    33361 ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca
    33421 tgcaggtaac gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta
    33481 tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt
    33541 actgcaagat caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa
    33601 ggatgtagaa gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt
    33661 gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga
    33721 tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa
    33781 agcggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg
    33841 caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta
    33901 cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt
    33961 gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat
    34021 gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta
    34081 ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc
    34141 tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct
    34201 gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag
    34261 atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat
    34321 ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa
    34381 acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa
    34441 ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa
    34501 acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta
    34561 gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa
    34621 caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga
    34681 aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg
    34741 gcggtccaag gtgaactagc aatcattgcg tctgaaccag gcaaatagtt cccagatagc
    34801 tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata
    34861 aatagaagtc tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg
    34921 tctatgaaac aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta
    34981 agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg
    35041 cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc
    35101 agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct
    35161 tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg
    35221 ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccatgtgaa attcaaggct
    35281 ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata
    35341 attggaatcg tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt
    35401 gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta
    35461 aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa
    35521 ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt
    35581 ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt
    35641 ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg
    35701 ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt
    35761 caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt
    35821 gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt
    35881 gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa
    35941 acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct
    36001 tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca
    36061 gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta
    36121 aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa
    36181 agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa
    36241 gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa
    36301 aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact
    36361 atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca
    36421 aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa
    36481 ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg
    36541 gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc
    36601 cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa
    36661 aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga
    36721 agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat
    36781 tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga
    36841 cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc
    36901 gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga
    36961 tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg
    37021 atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt
    37081 agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt
    37141 ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt
    37201 gatcccaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac
    37261 attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct
    37321 ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac
    37381 aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac
    37441 gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt
    37501 aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac
    37561 tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga
    37621 agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa
    37681 tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat
    37741 cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat
    37801 cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt
    37861 gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa
    37921 aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg
    37981 tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga
    38041 agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga
    38101 gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt
    38161 actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg
    38221 cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg
    38281 gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt
    38341 ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc
    38401 gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct
    38461 ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat
    38521 catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca
    38581 gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc
    38641 taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg
    38701 agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga
    38761 tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga
    38821 ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg
    38881 tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa
    38941 aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc
    39001 aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg
    39061 gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa
    39121 ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag
    39181 cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt
    39241 ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc
    39301 catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa
    39361 agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa
    39421 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
    39481 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
    39541 ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag
    39601 gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat
    39661 gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga
    39721 gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg
    39781 gatgctatgt ttctttacca tactatccag taatagattg actagagaga ttacctgctt
    39841 tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa
    39901 actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg
    39961 caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac
    40021 acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag
    40081 tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt
    40141 tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
    40201 ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt
    40261 cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag
    40321 cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc
    40381 aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc taaaatctct
    40441 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
    40501 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc
    40561 cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt
    40621 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
    40681 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
    40741 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
    40801 tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
    40861 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
    40921 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
    40981 aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta
    41041 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat
    41101 ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc
    41161 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
    41221 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
    41281 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
    41341 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
    41401 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
    41461 aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag
    41521 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
    41581 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
    41641 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
    41701 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
    41761 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
    41821 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
    41881 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
    41941 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
    42001 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
    42061 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
    42121 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
    42181 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
    42241 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
    42301 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
    42361 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
    42421 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
    42481 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
    42541 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
    42601 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
    42661 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
    42721 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
    42781 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
    42841 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
    42901 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
    42961 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
    43021 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
    43081 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
    43141 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
    43201 cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga
    43261 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
    43321 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
    43381 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
    43441 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
    43501 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
    43561 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
    43621 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
    43681 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
    43741 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
    43801 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
    43861 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
    43921 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
    43981 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
    44041 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
    44101 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
    44161 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
    44221 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
    44281 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
    44341 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
    44401 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
    44461 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
    44521 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
    44581 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
    44641 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
    44701 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
    44761 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
    44821 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
    44881 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
    44941 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
    45001 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
    45061 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
    45121 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
    45181 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
    45241 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
    45301 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
    45361 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
    45421 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
    45481 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
    45541 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
    45601 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
    45661 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
    45721 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
    45781 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
    45841 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
    45901 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
    45961 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
    46021 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
    46081 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
    46141 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
    46201 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
    46261 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
    46321 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
    46381 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
    46441 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
    46501 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
    46561 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
    46621 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
    46681 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
    46741 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
    46801 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
    46861 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
    46921 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
    46981 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
    47041 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
    47101 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
    47161 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
    47221 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
    47281 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
    47341 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
    47401 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
    47461 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
    47521 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
    47581 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
    47641 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
    47701 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
    47761 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
    47821 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
    47881 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
    47941 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
    48001 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
    48061 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
    48121 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
    48181 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
    48241 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
    48301 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
    48361 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
    48421 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
    48481 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
    48541 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
    48601 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
    48661 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
    48721 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
    48781 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
    48841 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
    48901 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
    48961 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
    49021 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
    49081 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
    49141 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
    49201 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
    49261 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
    49321 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
    49381 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
    49441 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
    49501 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
    49561 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
    49621 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
    49681 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
    49741 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
    49801 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
    49861 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
    49921 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
    49981 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
    50041 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
    50101 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
    50161 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
    50221 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
    50281 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
    50341 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
    50401 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
    50461 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
    50521 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
    50581 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
    50641 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
    50701 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
    50761 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
    50821 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
    50881 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
    50941 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
    51001 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
    51061 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
    51121 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
    51181 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
    51241 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
    51301 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
    51361 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
    51421 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
    51481 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
    51541 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
    51601 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
    51661 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
    51721 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
    51781 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
    51841 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
    51901 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
    51961 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
    52021 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
    52081 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
    52141 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
    52201 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
    52261 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
    52321 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
    52381 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
    52441 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
    52501 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
    52561 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
    52621 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
    52681 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
    52741 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
    52801 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
    52861 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
    52921 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
    52981 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
    53041 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
    53101 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
    53161 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
    53221 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
    53281 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
    53341 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
    53401 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
    53461 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
    53521 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
    53581 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
    53641 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
    53701 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
    53761 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
    53821 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
    53881 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
    53941 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
    54001 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
    54061 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
    54121 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
    54181 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
    54241 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
    54301 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
    54361 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
    54421 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
    54481 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
    54541 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
    54601 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
    54661 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
    54721 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    54781 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    54841 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    54901 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    54961 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    55021 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    55081 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    55141 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    55201 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    55261 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    55321 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    55381 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    55441 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    55501 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    55561 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    55621 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    55681 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    55741 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    55801 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    55861 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    55921 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    55981 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    56041 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    56101 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    56161 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    56221 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    56281 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    56341 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    56401 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    56461 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    56521 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    56581 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    56641 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    56701 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    56761 tgtaggaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt agattacaaa
    56821 acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca agaacataat
    56881 ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc acaaggaagt
    56941 atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg tgggtgtgaa
    57001 tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa
    57061 tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta tctcgctttg
    57121 gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca tctaacacat
    57181 ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg tgttgatccg
    57241 acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca tcaaccaaaa
    57301 ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa attcagagaa
    57361 atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc aggattagta
    57421 gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac taccactcat
    57481 aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc attagcaaaa
    57541 aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca tgtgattgcg
    57601 ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata tagtgaacaa
    57661 atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg aaccagagtc
    57721 atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat
    57781 ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa aaactcgatt
    57841 ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac gccagcaatc
    57901 actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca
    57961 ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt acgcgaatta
    58021 actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta aacagaaaag
    58081 ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac aatatcggtg
    58141 atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag ggaggaaaag
    58201 tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc
    58261 ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta gaagaagtac
    58321 aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg gatactggcg
    58381 aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat aaattaccag
    58441 ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc catgataaat
    58501 tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat
    58561 caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg caacagctga
    58621 ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca ggagattcag
    58681 taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa
    58741 tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca gattcctatc
    58801 atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt tgggaccaat
    58861 atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa
    58921 atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat gtattaagag
    58981 atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac
    59041 gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag acaaaggcaa
    59101 cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa gctttgcttg
    59161 tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg agtttgacta
    59221 gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag gagagcttaa
    59281 aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact aactattatt
    59341 cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg
    59401 atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga aacaccgatc
    59461 actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc aatcttgcgc
    59521 gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa agtgggacat
    59581 atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt caaacttcca
    59641 gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac aggcggatca
    59701 gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt
    59761 ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga tattgacatc
    59821 tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc tggactaggg
    59881 gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa
    59941 cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc tttttcacag
    60001 ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt
    60061 ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt
    60121 ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc
    60181 agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc
    60241 aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc
    60301 tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt
    60361 ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc
    60421 ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata
    60481 gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc
    60541 tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata
    60601 aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact
    60661 gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc
    60721 accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta
    60781 ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac
    60841 ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata
    60901 ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc
    60961 catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa
    61021 aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct
    61081 tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta
    61141 ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat
    61201 atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat aactcctact
    61261 attcagtttt tgaagataga gtcagtatac atgaagagtt aaaataggca atgttttttt
    61321 atggatattt gtattgctaa a
//
LOCUS       DAZUKA010000002        59650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000002 DAZUKA010000000
VERSION     DAZUKA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.002"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..645
                     /locus_tag="ACG8PN_000061"
     CDS             13..645
                     /locus_tag="ACG8PN_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860248.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(779..904)
                     /locus_tag="ACG8PN_000062"
     CDS             complement(779..904)
                     /locus_tag="ACG8PN_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4860249.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1038..1931)
                     /locus_tag="ACG8PN_000063"
     CDS             complement(1038..1931)
                     /locus_tag="ACG8PN_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4860250.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2113..3039)
                     /locus_tag="ACG8PN_000064"
     CDS             complement(2113..3039)
                     /locus_tag="ACG8PN_000064"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4860251.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3124..3885)
                     /gene="pflA"
                     /locus_tag="ACG8PN_000065"
     CDS             complement(3124..3885)
                     /gene="pflA"
                     /locus_tag="ACG8PN_000065"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4860252.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4129..6360)
                     /gene="pflB"
                     /locus_tag="ACG8PN_000066"
     CDS             complement(4129..6360)
                     /gene="pflB"
                     /locus_tag="ACG8PN_000066"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4860253.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6637..9087)
                     /gene="parC"
                     /locus_tag="ACG8PN_000067"
     CDS             complement(6637..9087)
                     /gene="parC"
                     /locus_tag="ACG8PN_000067"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4860254.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9099..11144)
                     /gene="parE"
                     /locus_tag="ACG8PN_000068"
     CDS             complement(9099..11144)
                     /gene="parE"
                     /locus_tag="ACG8PN_000068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4860255.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11310..11744)
                     /locus_tag="ACG8PN_000069"
     CDS             complement(11310..11744)
                     /locus_tag="ACG8PN_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4860256.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11871..12509
                     /gene="plsY"
                     /locus_tag="ACG8PN_000070"
     CDS             11871..12509
                     /gene="plsY"
                     /locus_tag="ACG8PN_000070"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4860257.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12641..13516)
                     /locus_tag="ACG8PN_000071"
     CDS             complement(12641..13516)
                     /locus_tag="ACG8PN_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4860258.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13598..14389)
                     /gene="codY"
                     /locus_tag="ACG8PN_000072"
     CDS             complement(13598..14389)
                     /gene="codY"
                     /locus_tag="ACG8PN_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4860259.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14407..15807)
                     /gene="hslU"
                     /locus_tag="ACG8PN_000073"
     CDS             complement(14407..15807)
                     /gene="hslU"
                     /locus_tag="ACG8PN_000073"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4860260.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15821..16369)
                     /gene="hslV"
                     /locus_tag="ACG8PN_000074"
     CDS             complement(15821..16369)
                     /gene="hslV"
                     /locus_tag="ACG8PN_000074"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4860261.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16406..17311)
                     /gene="xerC"
                     /locus_tag="ACG8PN_000075"
     CDS             complement(16406..17311)
                     /gene="xerC"
                     /locus_tag="ACG8PN_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4860262.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17509..18885)
                     /gene="trmFO"
                     /locus_tag="ACG8PN_000076"
     CDS             complement(17509..18885)
                     /gene="trmFO"
                     /locus_tag="ACG8PN_000076"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4860263.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18854..20932)
                     /gene="topA"
                     /locus_tag="ACG8PN_000077"
     CDS             complement(18854..20932)
                     /gene="topA"
                     /locus_tag="ACG8PN_000077"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4860264.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21054..21911)
                     /gene="dprA"
                     /locus_tag="ACG8PN_000078"
     CDS             complement(21054..21911)
                     /gene="dprA"
                     /locus_tag="ACG8PN_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4860265.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21967..22734)
                     /locus_tag="ACG8PN_000079"
     CDS             complement(21967..22734)
                     /locus_tag="ACG8PN_000079"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4860266.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22727..23587)
                     /gene="ylqF"
                     /locus_tag="ACG8PN_000080"
     CDS             complement(22727..23587)
                     /gene="ylqF"
                     /locus_tag="ACG8PN_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4860267.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23847..24245
                     /locus_tag="ACG8PN_000081"
     CDS             23847..24245
                     /locus_tag="ACG8PN_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4860268.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24482..25024
                     /locus_tag="ACG8PN_000082"
     CDS             24482..25024
                     /locus_tag="ACG8PN_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4860269.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="ACG8PN_000083"
     CDS             complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="ACG8PN_000083"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4860270.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25810..26037)
                     /locus_tag="ACG8PN_000084"
     CDS             complement(25810..26037)
                     /locus_tag="ACG8PN_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4860271.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="ACG8PN_000085"
     CDS             complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="ACG8PN_000085"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4860272.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26572..27285)
                     /locus_tag="ACG8PN_000086"
     CDS             complement(26572..27285)
                     /locus_tag="ACG8PN_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4860273.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27288..28184)
                     /locus_tag="ACG8PN_000087"
     CDS             complement(27288..28184)
                     /locus_tag="ACG8PN_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4860274.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28342..29184)
                     /locus_tag="ACG8PN_000088"
     CDS             complement(28342..29184)
                     /locus_tag="ACG8PN_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4860275.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29316..29969
                     /locus_tag="ACG8PN_000089"
     CDS             29316..29969
                     /locus_tag="ACG8PN_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4860276.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30032..30559)
                     /locus_tag="ACG8PN_000090"
     CDS             complement(30032..30559)
                     /locus_tag="ACG8PN_000090"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4860277.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30579..31526)
                     /locus_tag="ACG8PN_000091"
     CDS             complement(30579..31526)
                     /locus_tag="ACG8PN_000091"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4860278.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31538..33421)
                     /locus_tag="ACG8PN_000092"
     CDS             complement(31538..33421)
                     /locus_tag="ACG8PN_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4860279.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33584..35662)
                     /locus_tag="ACG8PN_000093"
     CDS             complement(33584..35662)
                     /locus_tag="ACG8PN_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4860280.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35675..35950)
                     /locus_tag="ACG8PN_000094"
     CDS             complement(35675..35950)
                     /locus_tag="ACG8PN_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860281.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36312..36767)
                     /locus_tag="ACG8PN_000095"
     CDS             complement(36312..36767)
                     /locus_tag="ACG8PN_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860282.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36921..38129)
                     /locus_tag="ACG8PN_000096"
     CDS             complement(36921..38129)
                     /locus_tag="ACG8PN_000096"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4860283.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38241..38573)
                     /locus_tag="ACG8PN_000097"
     CDS             complement(38241..38573)
                     /locus_tag="ACG8PN_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4860284.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38721..39596
                     /locus_tag="ACG8PN_000098"
     CDS             38721..39596
                     /locus_tag="ACG8PN_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4860285.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39678..40259)
                     /locus_tag="ACG8PN_000099"
     CDS             complement(39678..40259)
                     /locus_tag="ACG8PN_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4860286.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40265..41524)
                     /locus_tag="ACG8PN_000100"
     CDS             complement(40265..41524)
                     /locus_tag="ACG8PN_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4860287.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41526..42572)
                     /locus_tag="ACG8PN_000101"
     CDS             complement(41526..42572)
                     /locus_tag="ACG8PN_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860288.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42807..43082)
                     /locus_tag="ACG8PN_000102"
     CDS             complement(42807..43082)
                     /locus_tag="ACG8PN_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4860289.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43325..44635)
                     /gene="der"
                     /locus_tag="ACG8PN_000103"
     CDS             complement(43325..44635)
                     /gene="der"
                     /locus_tag="ACG8PN_000103"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4860290.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44817..46046)
                     /gene="rpsA"
                     /locus_tag="ACG8PN_000104"
     CDS             complement(44817..46046)
                     /gene="rpsA"
                     /locus_tag="ACG8PN_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4860291.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46181..46861)
                     /gene="cmk"
                     /locus_tag="ACG8PN_000105"
     CDS             complement(46181..46861)
                     /gene="cmk"
                     /locus_tag="ACG8PN_000105"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4860292.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46910..47545)
                     /locus_tag="ACG8PN_000106"
     CDS             complement(46910..47545)
                     /locus_tag="ACG8PN_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4860293.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47611..49014)
                     /locus_tag="ACG8PN_000107"
     CDS             complement(47611..49014)
                     /locus_tag="ACG8PN_000107"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4860294.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49001..50032)
                     /locus_tag="ACG8PN_000108"
     CDS             complement(49001..50032)
                     /locus_tag="ACG8PN_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860295.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50063..50284
                     /locus_tag="ACG8PN_000109"
     CDS             50063..50284
                     /locus_tag="ACG8PN_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4860296.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50449..51186)
                     /locus_tag="ACG8PN_000110"
     CDS             complement(50449..51186)
                     /locus_tag="ACG8PN_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4860297.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51339..52679)
                     /locus_tag="ACG8PN_000111"
     CDS             complement(51339..52679)
                     /locus_tag="ACG8PN_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4860298.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52916..54439)
                     /locus_tag="ACG8PN_000112"
     CDS             complement(52916..54439)
                     /locus_tag="ACG8PN_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4860299.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54817..55428)
                     /locus_tag="ACG8PN_000113"
     CDS             complement(54817..55428)
                     /locus_tag="ACG8PN_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4860300.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55551..55676)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55758..56474)
                     /locus_tag="ACG8PN_000114"
     CDS             complement(55758..56474)
                     /locus_tag="ACG8PN_000114"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4860301.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56480..57049)
                     /gene="scpB"
                     /locus_tag="ACG8PN_000115"
     CDS             complement(56480..57049)
                     /gene="scpB"
                     /locus_tag="ACG8PN_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4860302.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57033..57803)
                     /locus_tag="ACG8PN_000116"
     CDS             complement(57033..57803)
                     /locus_tag="ACG8PN_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4860303.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57800..58171)
                     /locus_tag="ACG8PN_000117"
     CDS             complement(57800..58171)
                     /locus_tag="ACG8PN_000117"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4860304.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58190..59077)
                     /gene="xerD"
                     /locus_tag="ACG8PN_000118"
     CDS             complement(58190..59077)
                     /gene="xerD"
                     /locus_tag="ACG8PN_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4860305.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59173..59646)
                     /locus_tag="ACG8PN_000119"
     CDS             complement(59173..59646)
                     /locus_tag="ACG8PN_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4860306.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT        16256 a        12634 c        10174 g        20586 t
ORIGIN      
        1 tttggagata ttatggaaat tcaacgtaga accactaatt ttttacattt aggctctttt
       61 tttgcaactt tgacgatctt attttttcta tgttattcat accttaagac ctcacatgcg
      121 ccttctagtg cattaggctg gttgcttttg gatcattctg atatcacaga agccgaagaa
      181 aatccgtttt ttttgatttt gtcttctctt tgcggcttac tgttttctgt ttatttcggt
      241 gcctattttt ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt attgattgca
      301 tttaaaacgt tttctttttt agcaattctt ttttattcgt ttaatcacaa tacgcttttc
      361 ttctttatca tgaatggttt agtcgtgatt ttatccattt ttacttattt tattttttca
      421 ctcattttta gccaattgaa atgtccgctc ttctttcgat tagttcctgt cacctgtttt
      481 atttacagct tgttttcctt ttttgtcctt gctttaatgc cactgaccaa cccttcgacg
      541 atccaagtcg caggaaatct gttggatatg ctcttcatcg tcagtctagg tgtatttatg
      601 tggcaaagaa aaaacaaaca aaataaaaag cattttgaag cctgactatt acgacttcat
      661 aaatgctttt ttagttttaa acacttcaat ctaaaagaat aacccaagat tggataaatt
      721 attaatgact cgccattcgt ttcttagtga ttctggatta tcaaatggac ttcctaatct
      781 aatcagctag ttcttcccat aaaatgccat acttgactga atcataatat tgattattta
      841 aaaatctaac ttttcttatc actacttcct ttgtcattcc acatttctca ccattttttt
      901 gcattccttt gtttccagac catgtagtga atctaatagg tggtagatag gcaaaatact
      961 caaataattc ttttaaccat aaaggaagag aaaggctcct cctgcaagag tcagtataat
     1021 ttttgcttac tgctctctta ggttttactc actaaaactg ttccatattc tttgatataa
     1081 gaaaatgcat gcggtataaa tttcttgctc agctcagaaa agatttgtcg tttaagaaag
     1141 gccatttcct ccattgtcaa tgaaggatcc aatgttggta attcaatctc aaaattgttt
     1201 ttttcgacaa taggtaattc taatgtgaat gattcttctt cctttatagt attcgtcaca
     1261 agtagagact gatggaaaat actaggccgg cgatttttat atttattctt acgtatcctg
     1321 tcaacgataa cttctatttg atcaaaaaag tacgattcat ttacgtctcc aataagttcg
     1381 gctaattctt tgaatgcctt cgttacagga agctgattct tctgtacaaa aaagtaatta
     1441 gccaaagtaa gcgggaagtg ttctatagag gaaagtgtga taaaacggta cttctcattt
     1501 cctgcagttg tcctcagcaa ttctgtctct cggctgtttc ttactccata aaaatacgct
     1561 tctttccgtt catcttcgaa acaatacgcc caaataactt ctcccatcgc taatttttgt
     1621 tcatcaacag agagtctctt caattgttca gctatggttt cagcagccga agtggcttgt
     1681 tgcaagtaat ctttaggatc aaatattgga aagttcaaat gatatcttcg ttcttccctc
     1741 ttgattaatc ctgcatcgat caataaatca agaaaatggg aaaatttatt ttgcgaaaat
     1801 tcttttttca attctcttag aatcgtttct tgttctcgat gttcatatag ataatcggtc
     1861 aatgaagaaa aaagcggatc atcggtcagt ttttgataaa agttttcctt attttttgaa
     1921 taatagttca tatatgttct cttttctatc cgttttattt tacttaaaag gcttttacgt
     1981 aaaaaggagg aagaacaaga gtcataattg actgttattc tcctccattt cttcggcctt
     2041 tagtatgttc tatgtttctt tttatatcaa tcgcttatcc attcattttg aatgcttcat
     2101 actaccattt gattagctaa atacagccgt caattgaggt acgacttgtt ttttacgtga
     2161 aacgacacct ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag cttcttctac
     2221 tttttctttt cctttaccag caacaagtaa ttctgaatca ctgtctaaga tgtttgtgac
     2281 aactaatacg aacagatcgt agccatttgc tttcatttca tcttccattg ctgcttctag
     2341 ctctgcttgt cgatccatca cttcattaag gtccactgta ttgacttgag cgatacgaac
     2401 gttggaactg cccattggga aactttttgc atccaaatca agaagaaccg cagctgattt
     2461 atcacttaga tttgttcctg ctttcaacat ttcaagtcca taagtgttta gatcgacttc
     2521 agcgattgct gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg ttggtgattt
     2581 gaacaacaat gtatctgaaa tgatagctga gagcatgatt cctgcgattt ctttaggaat
     2641 tgttacttgg ttttctttat atagttttaa aataatcgta cttgtacatc caactggttc
     2701 tgcacgatag tacaatggat tagctgtttc aaagtttgca atacgatggt ggtctactac
     2761 agctaaaata tttacttcgc tgatatctga aatactttgt tggaattcat tatgatccac
     2821 aagcatgaca tctgcatttt ctgctttagc tgactctact actcgtggtg cagatagctg
     2881 aaagtggtct aatgcgtatt gtgtttcttc attaggtgta cctagagcaa ctgcttctgt
     2941 ttctttacct aattcatttt gcaagtgtga aaacgcaatg gctgccccga ttgcatctgt
     3001 atctggattt tggtgaccaa aaactaaaat ttttgacatg tcattcgctc ctttgtgatt
     3061 tctcctattt aataatagca aaaaaacgga aagaagcaat acttatcttc tttccgatgc
     3121 gacctatcgc gttaaatatt ttgtatactc ttctgtatga agcaatcgtt tagcattttc
     3181 gacacgttct tttgtaggtg gttcgatccc ttctaatgga tatttcaaac caagtgtttc
     3241 ccacttgtat tttcccatcg tatgataagg aagaatctct actctgtcta catttgacaa
     3301 ggtttgaata aagttgtcta gtcggatcaa atattcatca taatcacttc gctcaggaac
     3361 tagtacatgg cgaatccaaa ccggtttgtt gatttttgac aaatattgag ccatttctaa
     3421 aatattttca ttgtcccaat gcgttagttt tttatgctct tcattatcga tgtgtttgat
     3481 gtcaaataac agtaaatctg tatacttcat caattcttga aaacgtgaaa agaaaggttc
     3541 ttcatacgta aaaggttttc cgcatgtatc caatgtagta tgcatcttcc gctcttttgc
     3601 ttttttaaat agttcgatca aaaaatcgat ctgcaataga ggttctccac cactaacagt
     3661 aataccacct ttatctcccc agtaaggtct gaagcgttct gctttatcta aaagttcatc
     3721 tgctgtgtat tctttccctc cgccaatatt ccaagtatct ggattgtgac aaaattggca
     3781 acgcatgcgg catccttgca taaacacgac aaagcgcagt ccaggtccgt ctactgaacc
     3841 gaaagtttcg atagaatgaa catagccgat tgttttttct tccatttaag ttcacatctt
     3901 ttctttgatt tcattttaag tacaagttag ctgtcgaagc gaagctttct gaacaaaatt
     3961 tttcgcaatt gccgcttcgt tatgtgtaaa atttacaggg acggaacata caagtcctgc
     4021 ccctgtaaat cgtttctttc aaataagcat actgattatt cgattttgat gcgatggcta
     4081 ttcaacgtcc tttcgacgct aagtcgaaaa acttttttca cgaacttttt acattctgtc
     4141 gtgtgaagtt ctcatcaaga catctaattg ttgttctctt gtcaagtcac ggaatttaac
     4201 tgcataacca gatacccgga tcgtcaagtt cggatatttc attggatttt ccattgcatc
     4261 acgcaataaa tcattactga atacattgac attcaaatga taagctcctt ttgagaagta
     4321 tccatctaag acattacgca agttttcgat tcttgtatct tgttcttttc caagacctga
     4381 aggattgatt gtttgggtat ttgaaattcc atccaatgca cagccgtaat tcattttagc
     4441 tgttgagttc aggctagcta ataacccact ttgttccgca ttgtaacttg gatttgctcc
     4501 tggcgcaagt ggttttccag cttcacggcc atcaggagta tttcctgtat ttttaccgta
     4561 aacaacattt gaagtaatcg tcaataatga agttgttggt tttgagttgc ggtaagtttt
     4621 atgacgtttg atttgtccca tgaagtattc caagatccat gaaactaatt catctgcacg
     4681 gtcatcatca tttccgtatt tagggaagtc gccatccact tcaaaatcaa ctgctattcc
     4741 tttttcatta cggattgctt ttacttttgc atatttgatc gctgacaagc tgtctgccgc
     4801 atgagagatc ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat ccatcaaagc
     4861 cagctgagct gcttcataag aatatttatc atgcatataa tgaatgatgt tcaacgtatt
     4921 gacatatagt tctgctaacc aatccatcat attcatgaag ttatgtttca cttcttcaaa
     4981 gtctagtatt tctgatgtga ttggacgata ttctggtcca acttgggcgc cagtcacttc
     5041 atcgactccg ccattgatgg catataatag tgttttagcc aagttagcac gagcgccaaa
     5101 gaattgcatg tcttttccga caactgttcc tgatacacag caagcaatcg cacaatcatc
     5161 agaaccccat tgttcacgaa gctgattatc attttcgaat tgaatcgaag aactttcgat
     5221 agcgattctg ctagcatatg ttctgaatcc ttctggaagg tgttcagaat acaaaacagt
     5281 caagtttggt tctggtgaag gtcccatatt tgttaatgta tgaagcagac ggaaatcatt
     5341 ctttgtaact aatgaacgac catctaatcc catacctgca atggataaag tagcccagat
     5401 agggaatcct gagaataatt ggttatattc aggtgtacgc gcgaatttga ccatacgtaa
     5461 tttcattact aagtggtcga tcaattcttg tgcttctttt tctgtgatta ctccattatc
     5521 caaatcacgt tggatataga tatcaaggaa tgctgagata cgtccgattg acatcgcagc
     5581 accattttgt gatttgattg ctgctaaata tccgaagtat agccattgga cagcttcttt
     5641 tgcatttgca gctggtttag aaatatcata gccatatttc gcagccattt ctttcatttg
     5701 cgctaatgca cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt cttcactcat
     5761 tgtatgataa ccagtcatat catgatcttt tttcttttgt tccatcaaat agtcgattcc
     5821 atacaatgcc acacgacgat agtcaccgat gatacgtccg cgaccgtaag cgtctggaag
     5881 ccctgtaatg attttgtttt tacgagcagc acgcatttca gcagtataag cgtcaaatac
     5941 accttgatta tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat cgtttggtgt
     6001 atagccattg ctttttagcg cattgttagc catattgatt ccgccgaacg gcatgaatgc
     6061 ttgtttcaaa ggtttgtctg tctgtagacc gacgattttt tccaaatctt tatccaaata
     6121 gcctgcatcg tgtgaagtaa tagttgatac gatttctgtg tccatatcat agacaccgtt
     6181 acgctcgaat tggatatcat ttaacttttg caattcatcc cataattttt ctgttgcttc
     6241 tgttgggcct tccaaaaagc tgtcatcacc gcgatattca gtgtagttat tttgaataaa
     6301 gtctctagtg tctacgcctt ctttccattt ggtaccttta aatccattcc attgctccat
     6361 tgtatggacc tcctaaattt ctgttttatg taacagtttt tacgtgatta gtataacaca
     6421 gcaccctatc tttgcaaggg ttttcttgtt ttttatttaa caatttttat gttttattta
     6481 catatattta aatcaacgtt gacataccaa catttaagca ttgtgaacac tttcatcttt
     6541 ttttgcaaac gtataaggat ttattaaaaa tgaatgaaaa agagacgcca cctgtattaa
     6601 tacagatggc gtctcttcat taaaatacta caacagttac gactcgatga aaatctcagg
     6661 agcatcgata acttcgatga cttctccacc ttttttctca tccatcgcaa atgatccatt
     6721 cgatgttcga tcacctatag ggaaatcagt tgggtcaatc gttaactgct gccctttttg
     6781 attcatcaag gttatttctt ttgatcttcc ttccgacata tagataatac ggtgcggatt
     6841 ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt ggctgatttc
     6901 ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg attacgatcg gcgtatcacc
     6961 ttctgctaac acaagcagcc cgttgataat cctatctcca tctttcaaat tcatagactt
     7021 cactccagct gcctttgctc caacaacggg aacatctgtt aaaggatagc gcaaacccat
     7081 tccttgatgc gttactaaga aaacatctaa gcctggctga tcttctttca gatacacagc
     7141 aatcagttca tcttcttccg cctttagctt catacagctg agcggacggc tcttataggt
     7201 gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc aatccattct tgctgatgaa
     7261 tacaaatgtt ttctctgctt gaattttttg atatgggaaa acagctaaaa tcgattcgtc
     7321 tacagacaga ttggagattg tttgagaaat gtgctcacca gcatctttcc atttcaaatc
     7381 gggtagttca tggacagggc ggtagatcac atttgcttta tttgtgatca gaagaacatg
     7441 gtccaatgta ctcaattcac ctgagtatag caagtagtcg ccttctcgca tcccgatttc
     7501 ttctggtttg gaagcactat aagaacgaag gcttgttcgc ttcacatagc cttctcgcgt
     7561 cacactgaca attacgtctt cttgtgccac taagacttgc gtatcaattt tgatttcttc
     7621 gatttcgtct tcgattgtcg ttagacgagc ggaagaataa ttttttttca cttctcgcaa
     7681 ttcttttttc ataacagaga aaagctcttt gtcatttgaa aggattttat tcaactctgt
     7741 gatttgcgca atcaagcttt cggattcctt ttgaagctct gtgatatccg tgttcgtcaa
     7801 acggtataat tgcaatgtga caattgcttc tgcttgttct tctgtaaatt gaaagacgtc
     7861 gactaaattc tttttggcgt cttttttgtc tttactttca cgaatcgttg cgattacttc
     7921 atccagaatc gataaagctt tcatcaatcc ttctacgata tgctgtcttt tttgagcttt
     7981 ggctaaatcg aattgactac gttttgttat gacttgcttt cgatgctcga tataacttct
     8041 taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga ccatattgaa
     8101 attgtaattg atctgtagct cagtgttttt gaacaaataa ttcaaaattc cttgagcatt
     8161 ggcgtctttt ttcaattcga cgacaatttg cagacctgtt ctatcacttt catctcgtac
     8221 ttcagctatt ccatcgattt tcttattcaa acggatctca tccatttttt tcaccaaggt
     8281 cgctttattt acttcatatg ggatttctgt gatcacgatt tgctgtttat tacccttcat
     8341 tgcctctatc tttgttttcg aacgaagaat aacttttcct cgtcctgttt cataagcttt
     8401 tttgatttca tcttttcctt gcaagatccc tcctgttgga aagtcaggtc caggaatata
     8461 ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc atatagattg tgccatcgat
     8521 cacttcagct aagttatgag tcgggatttc tgttgcatat ccagcagata tacctgtcga
     8581 tccattgacg agaaggtttg ggtatcgagc tggaaggact gttggttctt tttctgtatc
     8641 atcaaaattc cacacgagat cgactgtttc tttctcgata tctgctagca tctctccact
     8701 taacttggat aatctggctt cagtataacg catcgcagca ggtggatcac cgtccatact
     8761 cccgttattc ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt ggctcatgcg
     8821 gaccatcgct tcataaatac tgatgtcacc atgaggatga taattcccca tgatatttcc
     8881 gacagatttt gctgatttac ggaatccttt gtcatacgta ttgccatctt tgttcatggc
     8941 aaataatatc cggcgttgca ctggcttcaa tccatcacgg atatcaggca gtgcacgttc
     9001 ttggataata tatttggaat aacgtccgaa gcggtcaccc atcacttctt ctaaagttaa
     9061 ttcttgtact tcttgtcgat tttccatcag gtgacctcct attctacttc aaataactga
     9121 ttgttttcat ttttatctgc tcgttcttca tctaataaat cattagaaac tgaaggagag
     9181 atttcagtat cctctttttt atctaagata ctgccatctt cttcgagcgt aaattgcacg
     9241 tgattttcga tccattttct tcgaggttct actttgtcac ccatcagtgt ggttactcga
     9301 cgttcagctt gcgcagcatc atcgatacgg acgcggatca acgtacggga agtcggatcc
     9361 atcgttgttt cccacaattg ttccgcattc atctctccaa gtcctttata tcgctgcagc
     9421 atatatcctt tgccgacttt tttaatcatt gctgccaatt cgtcatctgt ccacgcatat
     9481 tcaatgactt gcttctttcc ttgtcctttg gatactttat ataaaggcgg taacgcgata
     9541 tatactttcc cagcttcaat cagcggtttc atataacgat aaaagaaagt aagcagtaat
     9601 acttgaatat gggcgccgtc tgtatccgca tcggtcatga tgattacttt gtcgtaatta
     9661 cagtcttcga tagaaaactc aggaccgact cctgcaccaa tcgtatagat catcgtattg
     9721 atttcttcat ttttcaagat atcttgcatt ttagcttttt cggtattgat gacttttcct
     9781 cgtaaaggta gaattgcttg gaactttctg tctcttcctt gtttagcaga tccgccagca
     9841 gaatcccctt cgactaaata caattcattt ttcttagggt ttcgggattg tgcgggtgtc
     9901 aattttcctg aaagaagaga ttcgcctttt ttacgcttct tcccatttcg gctttcttct
     9961 ctggctttac gtgctgcttc tctcgcttct ctggctttga ttgctttacg gaccaacatc
    10021 tggctcattt cgctattttc ctgcaaataa aagcccattt gttcactgat gacgttatct
    10081 actacggtac gagcaactgg cgtaccaagt ttttcttttg tttgcccttc aaattgcaga
    10141 agattttcag gtacacggat agaaagtaca gcagctaacc cttctctgaa atcactacct
    10201 tccagattct tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc attataagat
    10261 ttcgtcatcg ccgctttcat accagcttca tgggttcccc catcttttgt ccgaacgtta
    10321 ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt attgataagc gacctctact
    10381 tcgatacctt ctttttctcc tgaaaaataa acaactggtg tcaatgtatc tttttcttca
    10441 ttcagataat caacaaattc tttgattcct tcttcgtaat gaaaaatttc cttgactggt
    10501 tcttctccac gaagatcaga aagagagatt ttaacgcctt ttagaagaaa ggcagattct
    10561 cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg tactgaatat tgtatcatct
    10621 ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt ttcctatctt tttcaaagta
    10681 ccgtcaggtt taccgccgtt tttaaatttc tgctgatact ctacgccatc tctaacgatc
    10741 gttacaatta gccatttcga taaggcattt accacactgg ctcctacacc gtgcagtcca
    10801 ccagaagttt tgtaccctcc ttgaccaaac tttccgccag catgcagcac agtaaaaatc
    10861 acttcaactg ttggtattcc ggaagcatgc attcctacag gcatcccacg tccgctatct
    10921 gcaacagtta tactattgtc ttcatggatg gtcacatcaa tctcactgcc ataacctgat
    10981 aatgcttcgt caactgcatt atcgacgatt tcatagacta aatgatgcaa gcctcggctg
    11041 tcagtggatc caatgtacat ccctggtctt tttctaactg cctctaatcc ttccaatact
    11101 tggatggacg agtcattgta ttcgttgttt actttttttg ccaaggcaaa cgctccttca
    11161 ataatacttt gcggcaaaac cgcaccacga ttatcatact agaaaaggtc gtgaaaaaaa
    11221 agagggtaga cgagaaatca attttaatta tctcctaacc gctttttcga cattcccctc
    11281 tatcgatcat ctcttctttg tggtcatttt tatttttggt tttctttcaa cttaccaagt
    11341 tcaatcttaa tacaacgatc cattacgata tctgtacgtc ctgcttttct tagcatctca
    11401 gctgcttctt tgttttcaat ccctaattgt gcccagaaca ctttagcatc agttttcaaa
    11461 aaatcacctg ctatttcagg taaaaattca ctccgtctaa atacatctac gatatcgatt
    11521 ggatcatcga tttctgtcaa attcgcatag actttctcgc ctagaatttc ttcaccagcc
    11581 aataatggat ttactggtat gattttatat ccattgtctt gcaatacctg tgcaaccatg
    11641 taacttgttc gttctggttt attgctcaag cctacgacgg caatattttt tgcttcttcc
    11701 aaatagttga aaatcgtttc ttgtgttgga ttttttacag acatttctgc caccccttgc
    11761 ttttcttttt attccatcta ttatacttgt atctttcatt ttttcaattg ttcctgttac
    11821 ctattcatgc taaaataaag gatagttttc aacttgaaag agggtaaaaa atgaaattga
    11881 tcttattact tattttagca tatcttttag gttctattcc ttccggtgtc tggattggca
    11941 aattgttttt caaaaaagat atccgacaac acgggagcgg taatactgga acgaccaata
    12001 catttcgtgt attaggcaaa acagccggga ttgttgtcct gcttatggat attttaaaag
    12061 gtacattggc tacttgtttg ccgatgatct tccacctttc tgggattaac cctctttggt
    12121 ttggggtatg cgccattctt ggccatacat ttccgatttt tgctgggttc aaaggaggaa
    12181 aagctgttgc gacaagtgca ggaatgcttt taggatttaa tcctgtattt tttgtttatt
    12241 cttgtatcat ctttatcgtt agtttatttt gtacaagtat ggtgagtttg agcagtatga
    12301 tcagtgcagt attgatcact ttatctacga tcgtccttcc ttatgtagct cctgtgattt
    12361 tggctaagcc caattggtta ctgacgatca ttgccttttt agtttcttcc tttatctttt
    12421 atcggcataa agacaatatc aagcgtatca gaaaccatac tgaaagtcga atcccgtttg
    12481 gtttgaatag cagcaagaaa aaatcctgat actttttcat tccaaagaga taaaacggga
    12541 gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt tacacacctt
    12601 ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact gtgatggaat
    12661 attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt tccatcaagt
    12721 ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa acgaaaggcg
    12781 cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag cttaaagtta
    12841 cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt gtttcaaaaa
    12901 ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt gtctgaccta
    12961 acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat cttgatttca
    13021 atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga ggtacattga
    13081 aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt gccacttgat
    13141 aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca aaatcaaatg
    13201 gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt tcttgtccat
    13261 gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt tgatatgttt
    13321 tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat aaaaccggtg
    13381 catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca tgttcttttg
    13441 atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc tggtcatttt
    13501 gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa tgggaaggct
    13561 ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt tttgctaact
    13621 cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct aacgaacgag
    13681 attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca gaacgagtga
    13741 tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca tccaatgctt
    13801 caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt gccatttgga
    13861 cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa aggatctgca
    13921 taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca aacgattgtt
    13981 ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc ggaacgacag
    14041 tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta atatcactag
    14101 taattgggat attagcttca gttttcatca actgtgccac tgtatcagta taagattcag
    14161 gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg tgacgttcat
    14221 tataacctag taatactccg tcacttccta tgatataggt gttgctgtca agaataccac
    14281 ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa gtattctttt
    14341 gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt gtcgtcctcc
    14401 gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc caagtttgtc
    14461 atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg cttcaaataa
    14521 tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga tattatctgt
    14581 gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt ttgtaaacgt
    14641 aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag cattatttgg
    14701 ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta cccgtatcgg
    14761 gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga aagctcctga
    14821 agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta cttgtgaacc
    14881 ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc cagattgctg
    14941 tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt cttccgctaa
    15001 tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt cttccttgat
    15061 caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct tcggtgtcaa
    15121 agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt tcatcattgg
    15181 tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca gtaaaccctt
    15241 ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca cttcttcttg
    15301 cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt caaattgatt
    15361 agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca aacgtttttc
    15421 tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta cgatttggat
    15481 cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat aaccgacttc
    15541 agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg ccaaacgtcg
    15601 tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt ttggtgtaat
    15661 atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca aagcaacggc
    15721 aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac ttacgatttg
    15781 tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct tctacgataa
    15841 tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt tctgcaattt
    15901 ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca gccaacgcaa
    15961 aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact tctcctgtac
    16021 ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca agtttttgca
    16081 tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt agattcccgt
    16141 tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct gcaacacttc
    16201 cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt gcagtgcctt
    16261 tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca aatttcccgt
    16321 ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg tttgtttcct
    16381 cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga tagttttttt
    16441 gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg gcatgtccta
    16501 gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta gcaaatgtat
    16561 ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc atttgattca
    16621 agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca aaaacgatat
    16681 catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa tattcttgca
    16741 gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct ttaccatgga
    16801 tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta cactcactta
    16861 aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa tccaaaggac
    16921 tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa aaacgtggca
    16981 gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt atcacttctt
    17041 ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg atagaatttc
    17101 tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc gtaaaatatt
    17161 ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa tggagaatgt
    17221 cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc aaataacgca
    17281 aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt cccagagaat
    17341 aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc aatatttcta
    17401 agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt aagaaaaacg
    17461 caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt actcatttgg
    17521 gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag ctcgttgtgc
    17581 aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag gaaacagacc
    17641 atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt agtaagccat
    17701 acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg ctaagcgtgc
    17761 ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt ctacaccagt
    17821 catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg gtttcagcaa
    17881 ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga attcagcatt
    17941 ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca agtgagtctg
    18001 aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct ggataacagc
    18061 ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag gtccgaaaag
    18121 catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt caaaatattt
    18181 ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct catggaaagc
    18241 tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt tgtcatagcg
    18301 ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg gtgcagctgc
    18361 atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg ctaatggatg
    18421 agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag taatttcttc
    18481 gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt cagaaaaaga
    18541 atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg ccgaagtgat
    18601 tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat tagccaaact
    18661 gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt gatgcgcctc
    18721 agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt gtgcgatctg
    18781 ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt tcaatgaaag
    18841 aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca cagactactt
    18901 gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga caatttctgc
    18961 ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa ccatagaata
    19021 gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact gggcaagtca
    19081 caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta ctgcaagcat
    19141 aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca tcacaatcga
    19201 atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca ttcgtcagtt
    19261 ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc gtgccttctt
    19321 caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg atttgaggga
    19381 agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca aatctttttt
    19441 gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca taagtggaag
    19501 gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta aaacgtgctg
    19561 gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca tcgccttctt
    19621 caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct tccacataca
    19681 cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg acaccatttt
    19741 gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta gccacaaaac
    19801 gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt ttgagttcat
    19861 ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt gcgccttgag
    19921 catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta tcttcaataa
    19981 atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta cgcatgtatg
    20041 taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat tgctgtgcta
    20101 ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt tgcaggctac
    20161 ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact tttttaactt
    20221 cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca tctggtaatt
    20281 tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga gcttttttaa
    20341 aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct ttttcacggt
    20401 caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct ttcttgacct
    20461 tacgccagag aataggactg atcgaataac caacgattct gtctaaggta cgacgagctt
    20521 gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct gctttgactg
    20581 cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc aacccaagta
    20641 ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt gccaagtaaa
    20701 ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct tttccgcgta
    20761 tagaaatata atggggttca taattgtttt caaaatcgat acccatttta cttttcggta
    20821 aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga tatttctcaa
    20881 ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc attactgaat
    20941 ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa atcgtagcta
    21001 atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg gtgttattct
    21061 ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc ttggatcaaa
    21121 tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa aactgttctt
    21181 ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt ggcttcaaag
    21241 acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa acaatgcttt
    21301 ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc aatcacctct
    21361 tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat gactgcgatt
    21421 gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc tttcgcgcag
    21481 ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata aactgttttt
    21541 ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca agaaagtaaa
    21601 gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag cagctgaggg
    21661 ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt gccccagtta
    21721 gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat tttacctatc
    21781 ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca gttagcaagt
    21841 gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa attttcttct
    21901 atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg taaaagcggc
    21961 tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga ttggcgtaat
    22021 tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat tatgcgagaa
    22081 accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat atactttagc
    22141 aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta ttttttcctg
    22201 ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta caatggacaa
    22261 tttttctact gcttggatca tcgctaattt acttgcttca taaatattca catcatcgat
    22321 tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa cttgttgata
    22381 aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta atatgatctc
    22441 atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc ctcttcctac
    22501 ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt caaagcaaaa
    22561 catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt caaaagaagc
    22621 cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg taggatcatc
    22681 tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag tcatcacttt
    22741 tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct tgacgaatat
    22801 catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt tttgctgtaa
    22861 tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa ccataacgat
    22921 cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc gctaaatcat
    22981 ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg ccgatttctt
    23041 catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac tcattaccta
    23101 aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt gcaattttct
    23161 tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct aagaccattg
    23221 cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt tctttcaatg
    23281 cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc gtgatagcta
    23341 atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt tgtggatctg
    23401 ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc tgatctagca
    23461 ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg atgtccacat
    23521 atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga aaccattgga
    23581 tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta cttgtttttt
    23641 tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt agttaagtga
    23701 aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa atttttattt
    23761 tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag atgatagacg
    23821 aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat tttcctgttg
    23881 ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa aatgaagaat
    23941 tttctactga agaagctggt tcaatggtat gggatgacac tttttggata atgcttctct
    24001 ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat aaaaaaacaa
    24061 gtggtgcttt gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa
    24121 ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa
    24181 tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga
    24241 tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga
    24301 taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa
    24361 gcagtcttct cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt
    24421 catgacacat tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat
    24481 tttgaacact ttgattgcaa aagacgtttt tagattgccc gaaatgaatg caagattgtt
    24541 gtcgacctct gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc
    24601 tgcttatttt caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg
    24661 accgatctgg gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg
    24721 ttcaggaacg tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg
    24781 cttattttta tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat
    24841 tatgatccca gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga
    24901 tttcaaagta atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat
    24961 aatggttgtt tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt
    25021 ttgattttac gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct
    25081 caacagactg cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa
    25141 tagacaaatc ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta
    25201 tcttttgaaa ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat
    25261 tgtcccgtta gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat
    25321 ttcaggtttt tagttagata aggttcctta actactttcc cattgatctt caattgatca
    25381 ttttggtagc tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca
    25441 tctgctaatt gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca
    25501 accatatcac cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg
    25561 aataaaaatc cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca
    25621 agccagaagc gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt
    25681 tttttcattc tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa
    25741 taaaaataat tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa
    25801 aactatctct tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat
    25861 atccatggac ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct
    25921 agagctgtgc ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc
    25981 atcatgtgtg ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct
    26041 tcgttcctcc aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt
    26101 tcaggatgct ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct
    26161 ggttcgattt ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta
    26221 cttttttcag caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg
    26281 tcaccgcgat cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt
    26341 tctactaatt gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta
    26401 tgtccagtcg tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta
    26461 tatcctgaca tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag
    26521 aagcagcctc ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc
    26581 cgatgatttt ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt
    26641 atttgagttg tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc
    26701 atcatttact aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg
    26761 atccaaccta agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt
    26821 atagtttctc ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga
    26881 aagacttcta gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa
    26941 atagaaatcg ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc
    27001 caaataaaat ttatattcaa tatcactatc tttttgatac tcactaagga aaaagctgat
    27061 gatttgattg actttttctt tatttgaatt gatcgttaag acaggatctc cttgtctttc
    27121 cgtaagagct ggtatttctg ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa
    27181 aaatacggtc gaaccaagta ctaccccaag aaggataagg aaggcgaatt tccagtaatt
    27241 tgttttcttt gttttgtttg aagatacgtc agatcttgtt ttcatgttta ttgatcatcc
    27301 tccttctcta accattggtt ttgtgtctcg atcagctgat ctctcactgc atttgccatc
    27361 aattgataac ctaaattatt tggatggaaa tgatcttctt catagagcac attatttttg
    27421 atgccattta gattttctgt cgtgctagtt gtttgatctg ttccttcttc tgtgacacct
    27481 acttcctgat tcaatccttg ataaagcaaa tcattgattg ggataaagta gcagttcggc
    27541 attttttttg tcaccgtttc tgttcctttg ttccaattgt taacaattgt ctgcatgtca
    27601 gtgatctcag gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg
    27661 gttttattga gggatcgaaa ttcttccaat agttctgtga cattttcctg atagtttttc
    27721 agtggctttt taaaggaatt gaccgttaac ccgaataaat tattttggat aactttcatc
    27781 aaatcattac cgccaacagt cagtgtgata aaatcagctg ttttgatatt gtttcgaatt
    27841 tcttcttttg tttttactcg ctttaagatc tggtcacttc gttcaccatt tacaccatag
    27901 ttttctgttt caaccgcggt caaattataa cggtctttta gatcatttgc gacaagggga
    27961 acgaatcctc cttgcttggt ttgatctcct actccttcag tcagcgaatc tccgatagct
    28021 atgtagtgaa gtttttcttt tttatttgga tcttctctca cagtagttac tggtttcatt
    28081 agtgggccag ctttcggtat agaagcagct aaacctgcat agatgatgat cgcaattaat
    28141 aatgggaaca aaacgaggat caaatgtcgt ttcgttaatt tcattttttc acctcttgta
    28201 ttcgattggt tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta tgaaaaaaga
    28261 aaacaattat tttcctaatt ttataaagaa aaagagaata agctgcaagc ctttcaggct
    28321 gagggcttat cctcttttcg actaatctac ataatacatg attgcaaacg cgtctttacc
    28381 agtatgcgta gcgataaccg gattcgtatg cagtacaggg atatgcatgt ccgggaataa
    28441 ttcttgcaaa ccctctttga attcttgcgc caattctttt gcatccgcat gtgaaatacc
    28501 gatttgtcgc acatttggca attctttcag ttcatttttc aaattattaa accatttatc
    28561 gaacgttttc tttcctcgcc ccttagttac agggattaat tcagaatggg cgaaattcat
    28621 caccacgcgt atgttcaaaa cactagacag caaaccagtt gttcggctga ttcttccacc
    28681 ttttaccaag ttatctagtg tggatattcc gatgtataat tttgtattgt cttgtacttt
    28741 tttgacttct tcaaggattt tctctactgt tttaccttct tttgctaatt cagctgctcg
    28801 aattacctga aaagataatc cttggtcagt ggtatcactg tcgataaccg ttactttcgt
    28861 ttttgttaga ttactggctt gtcttgctgc ttctactgtt ccactcaagc cttttgtcat
    28921 atggatcgaa atcacttcac tgccgtcttc tcctagctca tcatacatcg agatgaattc
    28981 tccaattggt ggttggcttg ttttgggcag attttttgct ttcgccatca tttccataaa
    29041 tctttcgccg tctagttggt catcatcggg atatactgtt ccgtcgatca tgatggatag
    29101 cggcatgaca cgaatgccta aagcatcgcg tacatttttt tccatcgtac atgaagaatc
    29161 tgttacgatt tttacatttg tcattatttt ctctctctct ttctttaaca agatgcctta
    29221 ttcatgttaa actacatgta agacaatttc tatttttagt ataacagaca aataatgatt
    29281 tttcattaat tacacctagt aaaggagccg ttttcatgga aaaaacgaaa tttaatcgaa
    29341 cgtatgaaat attaaatgaa gtgtttaatg cagtcaccca tggaattggg actggattga
    29401 gtatcgcggg actagtggtt cttttagtaa aaggtgccca cttgcattcc tccctccata
    29461 ttgtctctta tgctatctat ggatcgatga tgattctttt atttttaacg tctactttgt
    29521 ttcattcatt gatttttaca aaagcaaaaa aggtgttcca agtattcgat catagttcga
    29581 tttttctttt gattgcagga agttatactc ctttttgttt gctgagtatc cgaggatggc
    29641 taggttggac tttatttgta ttaatctggt tgtttgcgat tcttggcatt atatataaag
    29701 cgttgacgtt gcataaacgg gagagagtat caaaagtatc tacgatcatc tatattttta
    29761 tggggtggct ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga atgactggtg
    29821 ttattttgat ggtagcaggc gggctttctt acacgatagg cgcattcttt tatagcttga
    29881 attctatccg ctatatgcat gttgtttggc atttgtttgt tatgttaggt gcaggtttta
    29941 tgtacttctc tgttttattc tttacttaat aaaaaaaata aagctatgga tgggacagct
    30001 ctaaataaca gctagtctat ccatagcttt tttatatcgt caattcgcct gtcccgcttt
    30061 ttacaggatt ctcattacgc tgatacgttt caaattgatg cggataacga tttttttcat
    30121 ccatttctcc ttgactagta ttgatcagtg accaatcaga ccagtcaaca ggtggaaaat
    30181 aagcatctcc atcgaataca tgatggatga ctgtacggta taatatcgta caaaatggca
    30241 gaaattcttg ataaacagct gaccctccag cgatgaaaaa gataccgtct gtttcttttt
    30301 ccagagcaag cacgtcttcc acactatgca tgacatgcac gccttctgct ttataggaag
    30361 aatctctcgt caaaacaatc gtctgacgat tcggcaacgg acggctaccc atcccttcga
    30421 atgtttttcg tcccatcact agtgtatttg cttctgtcat ttgtttgaag aatttcagat
    30481 catttggcaa acgccaagga agacggtctt cttttccaat caatccgttt tcatcctgtg
    30541 cccaaattgc agctaacatt ttctcatcct ttctgattct aaacagcgat aggagctttg
    30601 atcgccggat gtgggtcata tccttctaat tggatatctt ccatctcaaa atcaaatact
    30661 gactgctttt cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg gctcaattgt
    30721 tctttcattt gttcgatatg attgatataa agatgagcat ctcctaatgt gtgaacaaat
    30781 tcgccaaccg ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc ataacttgca
    30841 atgttgaacg gaacgcctag aaacacatcc ccactgcgtt gatacaattg gcagcttagc
    30901 cgtccctctt gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc catgctagga
    30961 acatcttccg gattccatgc agaaacgatc aatcttctag aatcaggagt ctttttgatt
    31021 gcttcgatca cttgtttgat ttgatcgata aagccaccgt cttttgtttc ccagtgacgc
    31081 cattgatagc cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga atcatcggtt
    31141 aggatcttct cgcaaaaaag cttgctctgc tttttatatt cttcattgaa ttcaggatct
    31201 gtcaaacatc tatggccaaa atcagtcata tctggtccat tatagtctgg acttttgatg
    31261 tagcgttcaa atgcccattc atcccaaata tggtttttat tttgtaatag atagcggata
    31321 ttcgtatctc cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa aggtactcgt
    31381 ttcgttgtta acaaaggaaa ccctttttgc agatcaaacc gcatttggta tccgaataag
    31441 cttaatgtgc ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa caattttctt
    31501 cctaagtcaa gatacgcttg ttccatcctt tttcctccta atcttctacc cattcactca
    31561 agtattccca ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat acgtcgtttt
    31621 gcaattcttg taatcgagtg aaattatctc cttgatgatt catctcttct tggagcaatg
    31681 agattttttc ttcaagttct gcaatctcat cttcaatcgt ttcccattct ttttgttcca
    31741 tgtaagataa tttcttcttt tcagcaggtt ccttgctctg tatttttggt ttcgcttttt
    31801 cagcaggctg ctgggtctct ttttgcttca gtaaataact ggacatgttt ccgtaataaa
    31861 gttcttgttt tccgtttcct tggaatacta ataacttatc tgctaccttg tccaagaaat
    31921 aacggtcatg ggaaacagtg atcacagcac ctggaaaact tctaaaataa tcttccaaaa
    31981 tcgtcaatgt tgctatgtcc aaatcattgg tcggttcatc caataataaa acattaggtt
    32041 gctgaatcag cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa agtttgccta
    32101 tcagtgtccc atgcataaat cttggaaata aaaatcgttc aagtaattct gctactccga
    32161 tttgactgcc atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa gcaatcatcc
    32221 gtttatctgg tgccatttct tcattttctt gtgtataata cgcaagatga accgtttcac
    32281 cgatagacat tgtaccgcta tccaggggaa ttctgccagc tagaatatta agcaatgtcg
    32341 attttcccgc accattttta ccagtaatac ctagtctttc ccgagattga atcaataaat
    32401 ctaaatgttc tagcagtgtt tggtcattta tacggtaact gccatctttg atttccagta
    32461 cttttttccc taaacgttgt gtcgctaggt tcagctctaa atcatcttct tggtttactt
    32521 ggtacaagtt ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc ttggtgccac
    32581 gtgcctgtac gccagccctc atccaagcta attcctgttt ataaagccgc ttgcgctttt
    32641 cttcttgttc tacttctaca cgatcacgtt ctgctttttc taagacgtaa gcttcataat
    32701 ttcctttata ctcgtataat tttccaaatg aaagttcgaa gattcggttt gctactctat
    32761 ctagaaaata acgatcgtgg gtaaccatca atagtgcgcc acggtattgt ttcaaatagt
    32821 tttccaacca ctcgatcgct tcataatcta aatggtttgt tggttcgtct agtaatagta
    32881 aatcgggttc atcaatcaac acttgcgcca gactaactcg ttttttctga ccacctgata
    32941 aagtactgat ttttttatct aattcactga ttcctaattt ttgcaaaata atctttgcgt
    33001 tggtatctgt agtccaagca tcttctttat tcatttgttc ttctgcttta gcataacgtt
    33061 tttgcacatc agggtcttct ccgttttccc ctagttcgat caatgcttcc tcatatgctc
    33121 gaacagtttg gatcaagggg ctgtttcctt gaaagactgc ttggagaaca gttaattcat
    33181 cggaaaattc agctgcttga gaaagataac caatacggta atcagttgga taaaacactg
    33241 tttgccgatc accgtcgctg ctatcgatac cggcaataat atttaagaga cttgttttac
    33301 ccgtcccgtt cgtcccaatc aacccgatac ggtctttttc gtggaccaaa aaagaaatct
    33361 ggtcaaacaa atctttttca ccataagttt ttttgaggtc agttactttt aattctttca
    33421 acatgttccc tccttatctt ggatattgta tcataaaaat agtgaacttc aggaaaattt
    33481 ttttggtatt tgttcttttt gtgttctcaa aaaaagaaca ggaccgattc gatcctgttc
    33541 tgatatgttt tgttttttat aaaacttgaa aatttttacc tttttatttt attggactcg
    33601 ttttttttga ctttgctcga aacgcaaatc ggaaagaaaa gttatcagat ggtttgttca
    33661 tcgtataaga ttctaacaaa ttgatattcc tgcgatagct gttcgcagaa aacgtcgtga
    33721 ttttttcagc aacttcgtat tcatcaatga tttttctttc aagataataa cctcttagca
    33781 tttttttcgt aaacagacta tcttgctgga tcatccacga agaaatcaaa ttccccgagt
    33841 tcatttttat tccttcaagt ttgcgttgct ggataaaaaa ttgaaccatc gtttcatcat
    33901 aaatatcagt caagttatct aaactttcaa taatcaactg agtatttttt tgatatgtct
    33961 ctctgatttc ttggatatct tcatcactaa taaatgtctg acgatcagca tatcttcttt
    34021 gccaaaacat tttcggatgg agaacgattc gcattaacct acggacaaca atgaaccaaa
    34081 agccgataaa agacaaaatc tggcttgtga tagaatgttc gaatcgagaa agaaaacggg
    34141 agtaaaaacg ataaccatta gaagaaagct tgcccgaacg ataactttca tccaatccat
    34201 cccgttcgat cgacaaaatc agtgcttgta tttcttgtac ttcttgcttt tcatcatcgg
    34261 ttaaatcttc caagtacaga tcacgtatcc gttcttgata attttctatc actgcttcag
    34321 tggctaaaaa ttcttttgta ttacgatcaa tttcctttat ttcttttcgc aaaacttcaa
    34381 cgacctcttc taaaatcatc aaagcattca aatccgtgct tttcgcttcc tctgtttccg
    34441 tcagcatagg caagacaatg atccctatga ccaaagtcac taagatgaca cccgccgtca
    34501 gaaacaaaag caaagaccgc tggtaaaaca tcatattatt gattgaaaat ggcaaaataa
    34561 aaatcgtagc caaactgact gttcccttta caccgccaaa tgttaagatc aagatctcgt
    34621 tcaactgttt tttgaacttt ttactgccat ctttgaatac ataaaaaaga ctgatagaaa
    34681 tgaatctgat cacaaaaagc atgacagaga ttaatacgat aatcgctaat aaaagaccgt
    34741 ttggatataa accgtctccc catactggcg agaacacttg cgtcaattcg attcctaaaa
    34801 atataaatac taatgcattc agtgtaaaag caatagttgt ccatgtactg tgggacaagc
    34861 tcgacaattc tgcatcaaac acagagattt tcctaaaacc agaagcttgg agaatacctg
    34921 ctgccactgc agcaatgatt ccagatacat caaatgcctc tgccaataca taggctagaa
    34981 aaggaagcaa aagttcgatc agcagatacc ctgtgacatc ttgtgctgat gctttttcga
    35041 tcaaatgaat tattttttgt ttgaaccaga caagcaaaaa gccgatcaaa gctcccccaa
    35101 tactagaaac tactagactc atccccgcgt ttactgctga aaaagatccg gtgatcagcg
    35161 caccaagagc aaactggaag gcagtaacac cagatgcatc attcaaaaga ccttctcctt
    35221 ccaaaatatg catcgccttt ttagggatat tcactcgtcc agaaagagaa ctgactgcta
    35281 ccgcatctgt cggacctaac gctgctccaa aagcaaagca ggcagcaagt gggacacttg
    35341 gcaacaggaa tgataacgtc gcacctaccc cgacaagggt caaaatgact cctccaaatg
    35401 ccaagaacaa aattgtcccg aaatttttga gaatcgaaga aatatctgct ttttctcctt
    35461 ctctaaataa caatggtgca ataatcataa ctaagaacaa ttcaggttca aaatcgattg
    35521 attgtcccca ctctgtcaga cctaatacca ctccaaggaa aatttggatc agtggagctg
    35581 gaatcattgg caaaatgcgg ctagctatgt tcgataaggt gatcgttact gcaaatacaa
    35641 taactaactc agcaaattcc atcgtccatc ccctttattt tttattttgt gtaagcattt
    35701 ttaaaaattg ttcttttttc tttaaaattt tggtttcgac tacttcaacc tgtgtttggt
    35761 tcccctctaa ttcacactgt acacgttttg actccaaacg aagcagatca tgtttcagac
    35821 gttccaactg acgatacatc aatatatttc ttttacgcaa cttacaaagt atttgacggc
    35881 ggtcatccat cgaaagggcg ccatagcgat catgcaacag ttttttttgt tgttcaaaac
    35941 tctctctcat tttccagatc tcctatacac aaacttccat cattatagca aagttctttt
    36001 tggatgaaaa atatttcatt gtttcatcgt ttattgctag ttagaaattt ccataaattt
    36061 ttcaacgcct cattctattc caaatagtat cagatatcaa gtaaaactta agaaagataa
    36121 aagtttctga caaaaggatg tttactttcc cattcctgcc tttcttgttc ttttatcaca
    36181 acaaaaaaat ggggctgcga caaaaatctt gtcacagtcc cttattctga ataaacggtg
    36241 tggaagagac agcctatcag tagtaataag acacatctct ttcacagcct catttttgcg
    36301 taaaaatcga tttattcgaa aaattccccg tatacatctt ctacacgttg atctggattg
    36361 atcacgatat acaagttacc tgttttttcg ctgatttttg ttgattgata ttgattgtct
    36421 aatccttctg ttcctttatc tagatatggg aagtacacaa gatcaggtgt gccatttcgg
    36481 taaaggattt ccattcgctc gatatcttcg tatttcaatg cgcgattaaa catgcccatt
    36541 tccagtccgc ctaggttgat atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg
    36601 attttaaatc cagcacacgg atggatttca acaaattcac tgccattgat tcgtccaaaa
    36661 cttgttgtga tttgtttgat ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt
    36721 tctccatttc taaaataaaa ttttagcgct gtcatttctt tactcattaa aaagactcct
    36781 ttatgatgta atttattctt gtactacctc agtttagcac aaatttatgt aagtggtcac
    36841 atcttatgtt ttttcttcta atgacgtatc tgcccacttt aagtagccaa caaagctgag
    36901 cagatacagt ctagtgtttt ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt
    36961 tttttatcat tttttattct ttgttctacg acggcttttt cagctaattg caaagcttca
    37021 ctaacccact taccaggttt ttttccagtt tcttctagca agattttacc atctactgct
    37081 aattcgctta atgacttgat aggtaaacga tcataggatt ctcctacggc atccatctga
    37141 ggtgtttcgt caaaatagat catcaattct tctacttgca gtgcatgttt tttgcctaag
    37201 aagtacaatt cgaatggttc caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt
    37261 gcttgaacgt ctttaatcat ttggttagaa cacttccacg atttcaaaaa ggaacgaatc
    37321 tcattttctt ttagatccag ttggctgatc aataatgccc atgcttggct ctcttctttc
    37381 aatgggacgg atggtaattc agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg
    37441 ggacagcaaa tgtagcactc tgtttcaata aatgcatgga ttcccgcggc ccgttcattt
    37501 cccaagagca tcttgatgaa ttccacattt atacgttcta cagatatttt acctaatagc
    37561 tcgtggtttt ctttgatggc gtagaaggtt tcttcctcta atttgaaccc tagttgactg
    37621 acaaaacgca acccgcgcat cattcgtaag gcatcttcat ggaaccgctc atgaggattg
    37681 cctacagctc gcaagatctg ttcttctaga tcttgcagac catcgaacaa atcgatgatt
    37741 tctccatctt ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt caaatcttct
    37801 gccaacgaac gaacgaattc gacatgatct ggtctacggt agtcctgata agttgattcc
    37861 gtccgaaatg tggtgatctc atattgttca tcattttcta ataccaacac agttccatgt
    37921 tcgatcccaa tatctatcgt ttttggaaaa agctgtttga tttcttctgg aaatgcgctt
    37981 gttgcgatat ccacatcatg gattggctgg ttcagcaaga tatcacgaac acttccacct
    38041 acaaaataag cttcatatcc tgcattctct aattttttca gaactggaat tgccttttgg
    38101 tattccagag gtaattgttt taatttcatt tttccaacct ttcctgcgtg tttcttgtcc
    38161 tgagataaag actcagttgc atggtgactg tttgacaagt ccgtttcaga gtcttcaagg
    38221 ccaggaccac ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg gtctctttga
    38281 ttgaattttt ccatgttttt ttcaaaaact tcagttaagt cgatattcaa tgaattagcc
    38341 atgatcatcg taacgaataa aacatcacca agttcttctg ctaccgagtt aactggttca
    38401 gaagcctttt tgcttttttc tccataagtg tggttgactt ctcttgccaa ttcacctact
    38461 tcttcagtta atcgagccat ctgagctaat ggagagaaat accccacctt gaattgctgg
    38521 atatattcat ccacttcctg ctgcatactg tataaagaac gctttttatc catctttttc
    38581 cctccaaaac tatcttacca aaactttaac gcaaatccta gataagagca attgagaaaa
    38641 tcgaatttcc atgctacatt taggaaggaa tttctaaatg gaaattctgt gaaaatacat
    38701 aattgaaagc tggaattcat atggtcaaac gaaaaacagt aaaagaagtt ctttttatca
    38761 ttataggaac aagtatttat gcatttggtc ttgtttactt gaacatcgct aatcatttag
    38821 cagaaggggg agtctcaggg atcaccttga tcttaagagc tttatttggt attgatccag
    38881 cttatacgac tttattaatc aacatcccat tgattttact aggaggaaag atcctcggta
    38941 aacgttcctt ggcctatact gttctaggta cactttcttt gtcattcttt ttatggatct
    39001 ggcaaagggt tccactagaa atcaatttac agcatgatct actgatcgtt tctctacttg
    39061 cgggtttagt tgcaggtgta ggaagcggga tcgtctaccg catgggtgga acaacaggcg
    39121 gcagtgatat tatcgctcgg attttagaac aaaattacgg catcactatg gggcgttccc
    39181 tattggcatt tgatatcatc gtcttacttg cttcattaac ctatatcgac ttgaaacgta
    39241 tgatgtatac gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg atcttagatg
    39301 gcggatattc tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag attgcaccac
    39361 tactgatgac cggtctgcaa agaggtgtga ctttcctttc tggagaaggc gcattctctg
    39421 gtaattcaaa aaaaatcatc tatatggtcg ttagctctag tgaattaact gaagctaaga
    39481 ggatcgtcca cgagattgac gaaaaagctt tcctttcaat catcaatgtg catgaagtgg
    39541 aagggcaagg ctttacttat ttgaagccac agacaagatt actgaaaaaa ctataagaaa
    39601 aaagacaaag ataggctatg aggaaagatt ccacatccag ctgacctttg tcttttttag
    39661 tactatacta tttttaacta attgttatgt tcacttaccc ggttactttt cttttgtgtc
    39721 tgcaaaagga tctttcggtt aacttcatca gataatttca aaaaagcatc cctatctcca
    39781 tcttctagtg cttgatcgat ttgagcataa agcattttta gttttgcctc ttcttctttt
    39841 tcttgaaaat atcgttcaat taattcagta tcttcttcac tgactgaaga atcccaagaa
    39901 gaaaatggat tgtcttctaa gatagataga tattctcttg tctgccaagc attttcgaat
    39961 atacattcca catataaagg ctttttccaa ttcaaacgga tttcatgaaa aatctggtct
    40021 gtgtccgtaa attcttttcc ctgcaaaaac aatttcatgg gttccccttc aacagataga
    40081 tcactgatct gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg gacattgctt
    40141 aaaatcgctt catgattggc aagataattc aagatccaaa aaacttctct tcggctaaaa
    40201 gatacatgat tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc aattagcatt
    40261 tttctcacct ctctgttagg tcatctaata atgacagtgc ctccatatct cctggttcaa
    40321 gtgttaaata ataagtcaat aattcttgcg ctcttttcag ctgcccttct tctcttaaga
    40381 aaaaggcata ctctttcaag aaatctggtt catgtgatag ttcatgataa gcctgttcat
    40441 agtgtacagc tgcaacggta aagtcttcta attcattata agcatgggct aggttccatt
    40501 ctgcataagg attagcagtt tcttccatct gattgatcac ttgtatcaca tcttcatatc
    40561 tttcatcatc caagtaaaga ttacttaaag taagtaatgt ttcatcttgt ttgtctccta
    40621 cttctaaagc tttcagtaaa agttcttctg ctcgctgtct atcatgcaga cggaaagcat
    40681 tttccgaggc caggtgataa agttcgactt ggtaaggatt ttctttgatt ccttcttcta
    40741 acactgtttg tgcttcttca atcatttctt cttcttgaag agcttgtcct agatatagat
    40801 ataacgattg ataatgcgga ttaacttctc gaagttcttc cagatagttg attgcctgct
    40861 gattttcttt caattgaaga ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt
    40921 cttctttcag tgcttcttct aaaggtacga tagctgcttc gaattctcct tgcatactta
    40981 aggattgtcc taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca
    41041 acaatgtttc ataaaccact gctgcctctt tgaaacgatc aactgaaaaa tacaattcag
    41101 ctagcgcaaa ctggatcaat ggttcatcag gaagcaaccc agctgcttct ttcaattttg
    41161 cttcactcac ttcagggatt ccgataactt ggtacaaatc agctatcgcc aataagcttt
    41221 gcggatagta atcactttgc ttatccactt tttccaggta ttcaaaagct aagtcgatct
    41281 cattatcttc gattgcaatt tctgctaacg ggatgttcag tccgtcattt tctgggtatt
    41341 ggttgatcag ttgttcaaaa atctgttttg cttcgatcaa aaagccaata gccagcaatt
    41401 cttcgcctag ttcagctaat atatctgctg ggtcttcttt taatgctttt tctaaaaaga
    41461 tttgtgcttg tgacaagtct tcatctgata aagcttgcaa cattttttcg ctatttgtat
    41521 tcatttcaat tccctcttat ctcactgacg atttgttgaa ataccgtcat tgttctcaat
    41581 aattcttttt cgtttggatg ttggtatccg tctattatac ctattttttc taaaacaagt
    41641 atttttttgc ttttttctgc caaaaaaaga actttttcca aatactccat ctttgaaatc
    41701 tgttccagcc agtggattgg atagccaagc aagtccagcc acacaaaaaa gttttttagt
    41761 tggaaatcaa aaggatatgc ttggagattc cacacgaaat gaaacatcat tcctaaaaat
    41821 tttttcatac tagcagaaag tagatggcca ttttcggtca gataaaaagc ttgttcaaac
    41881 agtttaccat aatttgacag gttttctgcc tctaattgat aaaaatagat catttcttcg
    41941 atcatcccgg caaacggccg agtcgttaat tgctttttat ttggatagtt tttatacaga
    42001 ttttgcaaaa aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc
    42061 tgctttccgt ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga
    42121 ttgatggttt gcagcactac acgatttggt agctgcaaaa tcgtccgctg cggtgttaat
    42181 gctcggctta gagaacggat cgatacaggg atcatccata gtgatgaatc aagggttgta
    42241 tgtttttgaa aaaaacctgc cagatccatg atcccttcat ttccaaatcc gatgataaac
    42301 atggattgat tttcgggata gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa
    42361 tgattgcatt gagtgttggc acaaatatac cagtcattgt ttgcttgatt tgcaaataac
    42421 gggttgatct tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa taagattggc
    42481 tgatcaatgg aaagttctct tagctgactg actagcgttt ctccgtaaaa cactttcgtc
    42541 aacgcatctt ctacttgata atcaagttcc atacgcatcc ctcctcttct tatttttatt
    42601 ttaccattag taagcaaatt ctagaaaaga taagagcaaa gctcacctca taacttgcaa
    42661 ataaataaaa attaaataaa atacatgtgt tttctatgta tgacatacgg attcggaatc
    42721 tctgtctctt atttattcct tttctgctag gaaataaaaa gaaaagcact tgtagcccta
    42781 gagccacaag cgctttttca actgtcttat ttaacagcgt cttttaatgc tttacctggt
    42841 ttgaatgcag gtactttgct agctggaatt tcgatttctt caccagtttg tgggttacga
    42901 cctttgcgag ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa ttgaactttt
    42961 tcgccgttag ctaaagcatc ttgaatagtt gaaaatacag catctactgc tgcagttgca
    43021 tcttttttag ttaaatcagt agctgaagca actttttcga ttaattctgc tttgtttgcc
    43081 atggatagat tcacctcctc cacaagagat atgatagaaa tatcatttag ttaaaacttt
    43141 ctgagtggtc cagaagttta aaaagaaata ttgattaact tcaatattct gttatcacga
    43201 tatcatatga accgcttaat agcaaggttt ttaccaattt tcacatgctt tttttagtca
    43261 tagaaacgta acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa
    43321 atgcctattt tctcctacga gggataattc ggatggacgt cccttcaaaa gtaaatgctt
    43381 ttctgatttg attttctaag aaacgttcat aagaaaaatg catcaattcc tcttcattga
    43441 caaaaacgac aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa atcttcaggc
    43501 gttttccttt atctgttggt gttggattga ttgctacagc atccataatg atatcattca
    43561 acacagccga agagatacga agattctggt tcatacttac ttgttcgatc atttcaggca
    43621 gacgttccaa ccgttgttta gtcactgcag aaacaaaaac gattggagcg taatcaagat
    43681 accggaattc atcacgaatc tcttgttcga aatcacgcat tgtattggtt tctttcttta
    43741 caagatccca tttattcacg acgataataa ttccacgacc agcttcatgg gcgtaacccg
    43801 ctactcgctt gtcttgttcc cgaatccctt cttcagcatt cagtaccatc aaaacgatat
    43861 cagaacggtc aatggcacgc attgcacgca tcacgctgta tttttcagta ttttcgtata
    43921 cttttccgcg tttacgcata ccagctgtat ctatcattaa gaatttctga ccactttcag
    43981 aaacgaagtg ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa acaattacac
    44041 gttcttctcc taaaatcgca ttgatcaaag aggatttacc aacatttggg cgaccaatca
    44101 aactaaactt aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa tacttcactg
    44161 cttcatctaa aatatcacca atccctagtc catggcttcc tgaaacagga taaggatcgc
    44221 ctagccctaa ggaataaaat tcatagatat cgtttctcat ttctggatta tctactttat
    44281 tgactgctaa aatcactggt ttattacttc tgtataaaat tttcgcaacc atctcatctg
    44341 cgtctgtcac gccttcgcgt ccgctgacta cacaaatgat tacatctgct tcttcaatcg
    44401 cgatttctgc ttgatgcttg atctgatcca taaaaggttc atcgccaaga tcaattccgc
    44461 ctgtgtcgat cacgctgaat tctcgaccta accattcacc ttttgcataa atacgatcac
    44521 gagttactcc aggagtatct tcgacaatcg agatacgctc accagcgatg cggttaaaaa
    44581 tcgtagactt accgacgttc gggcggccaa cgattgcaat tgttggattt gccatattat
    44641 ttcctcctca caagtttcca atctcactta gtttaacgaa tgcattgcca actttcaagc
    44701 atagattgtc aattaaacga agtattttta ttccttattg aaatactaat cgcttttcaa
    44761 aagaaaaact gagacaaaag cagctgcttt catctcagtc acagatacaa aatcaattat
    44821 ttttcagaat catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg
    44881 tctcccatag taaatccagt attttcttct ggtaattcat aagactcaac attttttggt
    44941 tcttcttctg aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct
    45001 tctggatgga tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt
    45061 ggtgtcgcaa tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg
    45121 aacacttcaa caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg
    45181 ccaactggag cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac
    45241 aaggaaacac gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg
    45301 acagccaata catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact
    45361 agaccgtcaa ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt
    45421 ccactgagaa catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt
    45481 tcgttttcca ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg
    45541 atgattttaa agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct
    45601 tccaccattg atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg
    45661 acaacatttg taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct
    45721 tcccaaactt ttttagcatc taaacggcgt tttgaaagta gatagctgcc gttttcttta
    45781 tctttgccga tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg
    45841 ctgatatctt ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca
    45901 gccccttcga ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca
    45961 acatcgccaa cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc
    46021 tcttgatttt gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa
    46081 gtacggtact gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt
    46141 ttccttgaaa aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt
    46201 ttttcaaaac aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat
    46261 cgatacgaac agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat
    46321 ggtctctttc ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt
    46381 ggttctcttt gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca
    46441 cttctgcatt cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc
    46501 gttcccctat ttttcgttgg acagcaacta attcttctct gacttttcca tgtgcagaga
    46561 cttcagaaac agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg
    46621 ctatgaatac atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca
    46681 gcgaaactag cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta
    46741 ctgcgcgata catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct
    46801 tggcaacggt acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca
    46861 tgatataact cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc
    46921 ttaattcttg accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca
    46981 attgatcagt ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa
    47041 cagtcgttgt tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc
    47101 ttgaatcccc tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa
    47161 tactgctttc tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac
    47221 tagattctac tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta
    47281 ctactgatgt cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc
    47341 tgatccagaa ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata
    47401 gcgaaagaaa taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt
    47461 catcatctgt atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag
    47521 attgatgtct atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata
    47581 ccataggaaa aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt
    47641 tagtaaaatc gttccccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag
    47701 aagttctgct tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc
    47761 gccaaaataa gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat
    47821 ctgctgcagg cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc
    47881 tggtttttca gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc
    47941 ttgctctgtg gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg
    48001 attaaagaaa taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc
    48061 ttcctgatct ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa
    48121 atcataatgg accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt
    48181 agcaatgaga aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc
    48241 tgcatcaaga ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa
    48301 tcgttcgact tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat
    48361 gaagtgttca agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg
    48421 ccgattgact gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc
    48481 tcttgctact aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag
    48541 ctttcggatt tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac
    48601 gcaatgagcc tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag
    48661 agaaggatta gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg
    48721 gaggacgtat cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc
    48781 ccctcgtttt tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat
    48841 gatgaccaga ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc
    48901 tgtaggcaaa atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg
    48961 gccgggacga aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta
    49021 tcgcttcctt tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc
    49081 catctacatt ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac
    49141 gttcgattgt ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc
    49201 actcaataag atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt
    49261 caattgtctg accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt
    49321 gctgtaataa tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg
    49381 ctaacaaaag gtggatacat ctgctttgtt gcagataaac cgctgactca tcattgtttt
    49441 ttgccagctg ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa
    49501 aatctgctgc ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat
    49561 caatcaaaat ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat
    49621 aaaaaggact tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta
    49681 cttgaacagc aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa
    49741 aacgcaaacc agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta
    49801 gctttgcttc attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt
    49861 gataaaactt atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg
    49921 taaatccata aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac
    49981 taatccgcag cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct
    50041 ccttaagtta ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat
    50101 tgcatgcggt ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat
    50161 cgttttattt gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac
    50221 atatgtaaaa aaagcgtatc aaaaatgtcc agttcgagcg attttattag aaaataaaat
    50281 ataaatataa acgaaacttt aaaaacgtac actaagttct ctaaacttga aaataagccg
    50341 ggatctgttt ctcttgttcc gtgaaatccc gacttatttt gaaagattca tttgctatct
    50401 atagttaatt gactagtttt ttcacggacc ctataggacc catttatatt atttcaactt
    50461 tttcagccag ttttccaatg tattgacgat cacagtttgc tggtctgcgt tagaaatttc
    50521 tgcctctcca tctccttttt gctcaccata gctgccaaaa cctccatgat ttccgccatt
    50581 gattgataca tactctgttc tttccggtaa atactgctta gcttcttgat aagcttcctg
    50641 attcaatact ttatcttttg tcgccgtgat cgataaaaca ggaacagctg attctgtcaa
    50701 tgttcctttt ttgtcgggat aacttgccat gaagaatacc ccttttaaat gtttgtctga
    50761 atgattctta ttgacataac gacttgccat tacgccacct aatgaatggc caccgatcac
    50821 ataatcggaa tttggaaaat cttctttgac cttatccgct ttgtttccgc ccatcactgc
    50881 tagatcaagt ggcattttga caatagcgac tgaaaagccg ttttttgcta aatcttgcgc
    50941 ccaaatacta taactttctg gctctacgag agcaccagga taaaaaataa gtaatggaga
    51001 ctttccatga ttctttttct ctccttcaaa aaataagtaa tcttctttgt tttttccttc
    51061 ttttaccaac acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta tatagtaata
    51121 accggctcca ccgagtaatc ctaaaatgat gactaagcca attagtattc gtctaaatat
    51181 tttcactttc tcccaccttt tctatctgag tgaccccttt ttcttaatca agtatgatta
    51241 taccttaaaa aaataagaga gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa
    51301 tatccatttg aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac
    51361 acagcttctg cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc
    51421 gcctggtgca taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc
    51481 atcgtacgag caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata
    51541 tcgcgatatg tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa
    51601 tcaaggaagt caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta
    51661 gctactaaat catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt
    51721 tgttctggtg tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct
    51781 tctgttttgc ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa
    51841 ttgattgggc caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc
    51901 actccttgtg ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct
    51961 tgttcttcga aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct
    52021 ccagagtaag gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac
    52081 tcgttcgttc ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa
    52141 ggaaattcag catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt
    52201 tctaaacaac gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg
    52261 aattccacgc cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct
    52321 ttgtcatctt gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt
    52381 tttgtttccc aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg
    52441 aataattcat ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat
    52501 gttctaaatc ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt
    52561 ttaccgaagg gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc
    52621 aattcgctca atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg
    52681 atattatcct cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc
    52741 ttctcaaata atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg
    52801 atgaaagaaa aaacactcac tgaatggagc tattgactta tccacgtgct tatcgatagc
    52861 cgtttatcgg tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga
    52921 acattgctgc tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac
    52981 cggtaaattt ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga
    53041 tagcagatgt tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta
    53101 ctaaatcctt accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag
    53161 cagctaagcc ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa
    53221 taatgatgaa tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg
    53281 tgtctgtgat taatccattg ttcataacca cttggatacc acgagcaaca gctactacga
    53341 ttgctacacc gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt
    53401 tcatgccata agtgattgct actacgatgg acattactaa gaataacata gtaatttctg
    53461 ggaagtacca gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa
    53521 ggtttgttaa ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc
    53581 aaggaatcaa actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt
    53641 tttgtttttt cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat
    53701 cttctttacg ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat
    53761 aacgatacac atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac
    53821 caataccttc acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat
    53881 ttaccgttga agctagacag cctacttgag accctactaa tacgattgct acagctacta
    53941 ctgtatcaaa gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt
    54001 cttctgccat tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa
    54061 gcattttttc ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc
    54121 ctgttttgtt gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga
    54181 ttgcagcagg tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg
    54241 gcgccatgaa aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag
    54301 cgacgatgtt gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta
    54361 cagcgattac tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt
    54421 tcttttttgt ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg
    54481 ctctaatgtt attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa
    54541 aacttttgtt caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag
    54601 ctttcttctt tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta
    54661 aaaggagaag aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa
    54721 atactcaaaa caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa
    54781 aaaaaagacc aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg
    54841 ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa
    54901 gacagcactc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata
    54961 gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag
    55021 aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt
    55081 aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata
    55141 aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc
    55201 cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc
    55261 ggccgctgga ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt
    55321 caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc
    55381 agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc
    55441 tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt
    55501 ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc
    55561 tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa
    55621 taggtcgtgg actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta
    55681 tataattttt gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac
    55741 ttataaaaag taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc
    55801 agttcacggt aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt
    55861 tttagttttt gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt
    55921 ccttcgtgga tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt
    55981 tggaatttag ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca
    56041 agcatcgatt ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt
    56101 tttggatgtg tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa
    56161 gtatcgtaat ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc
    56221 gtaacaactt ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac
    56281 aataaatagt aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca
    56341 tgtttcccaa cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct
    56401 gcaaggatga attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc
    56461 tgcaatctct ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg
    56521 acacgtcttc ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt
    56581 tcaaaccaaa ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc
    56641 cttctactct acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg
    56701 aaacctgaac tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga
    56761 tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc
    56821 gcttaagtaa gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa
    56881 aaatatgcaa agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac
    56941 ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt
    57001 cctcgccaac aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct
    57061 cctactgctg gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc
    57121 ccttttttca tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt
    57181 gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata
    57241 aataccattc gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga
    57301 gaaaatttct tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact
    57361 tgtcctcgct tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt
    57421 gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat
    57481 ttacggtatt ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct
    57541 tcatcgtctg tgacgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat
    57601 gtagcagcca tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg
    57661 atataattca tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc
    57721 atttgctgaa tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata
    57781 ttgatctccg tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt
    57841 aaggctctgt gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat
    57901 ctaacatttg aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga
    57961 tgatcagctg atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac
    58021 cttctggcgt aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct
    58081 tcaacaaaga agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt
    58141 tacggttttc tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg
    58201 gaaaatgctg cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag
    58261 tcgtagagat atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt
    58321 ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc
    58381 cagcctctcg tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc
    58441 catgattatt gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt
    58501 cgtctaagta gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt
    58561 tatctccttt tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca
    58621 atccaatcaa ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat
    58681 ctcttatccc taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca
    58741 gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat
    58801 catgatcagt gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca
    58861 tccgagtaac agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga
    58921 ctaaaaagcg atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt
    58981 actgttccaa atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat
    59041 tcaagtagtg gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct
    59101 atcatatcag ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag
    59161 ccgtctagac actcagacgg actggtttct tttttctttt tcatagcatt cctgacagat
    59221 tccatggaac gttagccgat gatcttttac taaaaaatga taacgttctt ctactacttg
    59281 ctccacttca agtaaaagat cctcttctac ttcctcgatc gtgccgcagt tcaagcatag
    59341 taaatgatga tggaaatgtt tggcgccttc ttttctcaaa tcataacggg ctaatccatc
    59401 attaaaactt actttatcga caattttcaa ttctgtcaag atttcaaggg ttcgataaac
    59461 agtagcaagt ccaatttccg gactcttttt tttcactaaa aagaagattt cttctgcaga
    59521 taaatgctct ttctcatttt ccaaaagcac aagcagagta gcttctcttt gcggtgtcag
    59581 cttaaacccc gcttcatgta gctgctgttt tgtttttttc atcgccgaaa tagcattcat
    59641 gaccatttac
//
LOCUS       DAZUKA010000003        50199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000003 DAZUKA010000000
VERSION     DAZUKA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.003"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            302..3109
                     /locus_tag="ACG8PN_000120"
     CDS             302..3109
                     /locus_tag="ACG8PN_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4860307.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3300..3791
                     /locus_tag="ACG8PN_000121"
     CDS             3300..3791
                     /locus_tag="ACG8PN_000121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4860308.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3894..4883
                     /locus_tag="ACG8PN_000122"
     CDS             3894..4883
                     /locus_tag="ACG8PN_000122"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4860309.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4916..5719
                     /locus_tag="ACG8PN_000123"
     CDS             4916..5719
                     /locus_tag="ACG8PN_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4860310.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5740..6654
                     /locus_tag="ACG8PN_000124"
     CDS             5740..6654
                     /locus_tag="ACG8PN_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4860311.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6737..7111
                     /locus_tag="ACG8PN_000125"
     CDS             6737..7111
                     /locus_tag="ACG8PN_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4860312.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7250..7495
                     /locus_tag="ACG8PN_000126"
     CDS             7250..7495
                     /locus_tag="ACG8PN_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4860313.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7667..8731
                     /locus_tag="ACG8PN_000127"
     CDS             7667..8731
                     /locus_tag="ACG8PN_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4860314.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            8977..9402
                     /locus_tag="ACG8PN_000128"
     CDS             8977..9402
                     /locus_tag="ACG8PN_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4860315.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9395..9844
                     /gene="nusB"
                     /locus_tag="ACG8PN_000129"
     CDS             9395..9844
                     /gene="nusB"
                     /locus_tag="ACG8PN_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4860316.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9941..10795
                     /locus_tag="ACG8PN_000130"
     CDS             9941..10795
                     /locus_tag="ACG8PN_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4860317.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10796..12133
                     /gene="xseA"
                     /locus_tag="ACG8PN_000131"
     CDS             10796..12133
                     /gene="xseA"
                     /locus_tag="ACG8PN_000131"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4860318.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12138..12362
                     /locus_tag="ACG8PN_000132"
     CDS             12138..12362
                     /locus_tag="ACG8PN_000132"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4860319.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12364..13254
                     /locus_tag="ACG8PN_000133"
     CDS             12364..13254
                     /locus_tag="ACG8PN_000133"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4860320.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13263..14081
                     /locus_tag="ACG8PN_000134"
     CDS             13263..14081
                     /locus_tag="ACG8PN_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4860321.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14188..14637
                     /locus_tag="ACG8PN_000135"
     CDS             14188..14637
                     /locus_tag="ACG8PN_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4860322.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14655..16337
                     /gene="recN"
                     /locus_tag="ACG8PN_000136"
     CDS             14655..16337
                     /gene="recN"
                     /locus_tag="ACG8PN_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4860323.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16392..16508)
                     /locus_tag="ACG8PN_000137"
     CDS             complement(16392..16508)
                     /locus_tag="ACG8PN_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4860324.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16581..17537)
                     /locus_tag="ACG8PN_000138"
     CDS             complement(16581..17537)
                     /locus_tag="ACG8PN_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4860325.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17708..18076
                     /locus_tag="ACG8PN_000139"
     CDS             17708..18076
                     /locus_tag="ACG8PN_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4860326.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18246..18677
                     /gene="mraZ"
                     /locus_tag="ACG8PN_000140"
     CDS             18246..18677
                     /gene="mraZ"
                     /locus_tag="ACG8PN_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4860327.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18698..19657
                     /gene="rsmH"
                     /locus_tag="ACG8PN_000141"
     CDS             18698..19657
                     /gene="rsmH"
                     /locus_tag="ACG8PN_000141"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4860328.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19662..20063
                     /gene="ftsL"
                     /locus_tag="ACG8PN_000142"
     CDS             19662..20063
                     /gene="ftsL"
                     /locus_tag="ACG8PN_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4860329.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20064..22256
                     /locus_tag="ACG8PN_000143"
     CDS             20064..22256
                     /locus_tag="ACG8PN_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4860330.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22282..23244
                     /gene="mraY"
                     /locus_tag="ACG8PN_000144"
     CDS             22282..23244
                     /gene="mraY"
                     /locus_tag="ACG8PN_000144"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4860331.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23258..24619
                     /gene="murD"
                     /locus_tag="ACG8PN_000145"
     CDS             23258..24619
                     /gene="murD"
                     /locus_tag="ACG8PN_000145"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4860332.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24645..25733
                     /gene="murG"
                     /locus_tag="ACG8PN_000146"
     CDS             24645..25733
                     /gene="murG"
                     /locus_tag="ACG8PN_000146"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4860333.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25748..26968
                     /locus_tag="ACG8PN_000147"
     CDS             25748..26968
                     /locus_tag="ACG8PN_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4860334.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27124..28449
                     /gene="ftsA"
                     /locus_tag="ACG8PN_000148"
     CDS             27124..28449
                     /gene="ftsA"
                     /locus_tag="ACG8PN_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4860335.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28472..29713
                     /gene="ftsZ"
                     /locus_tag="ACG8PN_000149"
     CDS             28472..29713
                     /gene="ftsZ"
                     /locus_tag="ACG8PN_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4860336.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29730..30407
                     /locus_tag="ACG8PN_000150"
     CDS             29730..30407
                     /locus_tag="ACG8PN_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4860337.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30421..31020
                     /locus_tag="ACG8PN_000151"
     CDS             30421..31020
                     /locus_tag="ACG8PN_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4860338.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31101..31322
                     /locus_tag="ACG8PN_000152"
     CDS             31101..31322
                     /locus_tag="ACG8PN_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4860339.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31371..32153
                     /locus_tag="ACG8PN_000153"
     CDS             31371..32153
                     /locus_tag="ACG8PN_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4860340.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32174..32881
                     /locus_tag="ACG8PN_000154"
     CDS             32174..32881
                     /locus_tag="ACG8PN_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4860341.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33143..35941
                     /gene="ileS"
                     /locus_tag="ACG8PN_000155"
     CDS             33143..35941
                     /gene="ileS"
                     /locus_tag="ACG8PN_000155"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4860342.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(35990..37516)
                     /gene="zwf"
                     /locus_tag="ACG8PN_000156"
     CDS             complement(35990..37516)
                     /gene="zwf"
                     /locus_tag="ACG8PN_000156"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4860343.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37531..38178)
                     /locus_tag="ACG8PN_000157"
     CDS             complement(37531..38178)
                     /locus_tag="ACG8PN_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4860344.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38362..38691)
                     /locus_tag="ACG8PN_000158"
     CDS             complement(38362..38691)
                     /locus_tag="ACG8PN_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4860345.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38868..39596
                     /gene="sfsA"
                     /locus_tag="ACG8PN_000159"
     CDS             38868..39596
                     /gene="sfsA"
                     /locus_tag="ACG8PN_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4860346.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39612..40625
                     /locus_tag="ACG8PN_000160"
     CDS             39612..40625
                     /locus_tag="ACG8PN_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4860347.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40625..41902
                     /locus_tag="ACG8PN_000161"
     CDS             40625..41902
                     /locus_tag="ACG8PN_000161"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4860348.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            41965..44667
                     /locus_tag="ACG8PN_000162"
     CDS             41965..44667
                     /locus_tag="ACG8PN_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4860349.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44819..45136
                     /locus_tag="ACG8PN_000163"
     CDS             44819..45136
                     /locus_tag="ACG8PN_000163"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4860350.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45166..45486
                     /locus_tag="ACG8PN_000164"
     CDS             45166..45486
                     /locus_tag="ACG8PN_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4860351.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45594..47054
                     /locus_tag="ACG8PN_000165"
     CDS             45594..47054
                     /locus_tag="ACG8PN_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4860352.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47122..47343
                     /locus_tag="ACG8PN_000166"
     CDS             47122..47343
                     /locus_tag="ACG8PN_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860353.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47374..47556
                     /locus_tag="ACG8PN_000167"
     CDS             47374..47556
                     /locus_tag="ACG8PN_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4860354.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47556..47969
                     /locus_tag="ACG8PN_000168"
     CDS             47556..47969
                     /locus_tag="ACG8PN_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4860355.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48092..49273
                     /locus_tag="ACG8PN_000169"
     CDS             48092..49273
                     /locus_tag="ACG8PN_000169"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4860356.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49344..49508)
                     /locus_tag="ACG8PN_000170"
     CDS             complement(49344..49508)
                     /locus_tag="ACG8PN_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860357.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49662..49735
                     /locus_tag="ACG8PN_000171"
     tRNA            49662..49735
                     /locus_tag="ACG8PN_000171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49696..49698,aa:Arg,seq:cct)
     gene            complement(49804..>50199)
                     /locus_tag="ACG8PN_000172"
     CDS             complement(49804..>50199)
                     /locus_tag="ACG8PN_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4860358.1"
                     /translation="ISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLVS
                     SNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMS
                     TYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        16705 a         8483 c        10819 g        14192 t
ORIGIN      
        1 gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt gtttgctatc
       61 agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata aaaatcgtat
      121 cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta tcaacaaaac
      181 aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt tttgaagcct
      241 aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg accgaattta
      301 tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc aaggagtaac
      361 aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta aagatctgaa
      421 tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac gttacatata
      481 tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt acaaagaaac
      541 gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct ttgcaaaaat
      601 cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg cgattttgta
      661 tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa agacttattt
      721 tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg aaacggaact
      781 gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga gtcatttatt
      841 tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga ttatcgatca
      901 agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg aaggtcaaga
      961 aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa caacgaaatc
     1021 ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt cttctctatt
     1081 agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc
     1141 tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa accgcacgca
     1201 gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg tgacctatgg
     1261 aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat ttctaaatca
     1321 tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga ttcgtagcgg
     1381 attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa tattggagcc
     1441 aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact cctatgagtt
     1501 accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt cggatggttt
     1561 ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc tgaagtcttt
     1621 ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat ttgtagatcc
     1681 gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt taccatatga
     1741 gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt tgaaaaaaat
     1801 caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt ttgattttcc
     1861 agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga
     1921 gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac gggcaaatca
     1981 agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag caagcagtat
     2041 ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag acatgccttt
     2101 ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag aagtagatga
     2161 aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta attcgcaaat
     2221 tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt ctctagtttt
     2281 ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc atgaatcatt
     2341 gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc
     2401 tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta cagcacaaag
     2461 aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat tagaagttct
     2521 agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag aatatcagat
     2581 catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc ctttagaacg
     2641 ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat cagaattgga
     2701 ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat
     2761 ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt ggcaattttc
     2821 tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca aaatcaacct
     2881 agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat tgactgtgga
     2941 agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg cagcctcagt
     3001 tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt attatctgct
     3061 acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa tacacaaaaa
     3121 cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt aaattcaatt
     3181 agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct aggaggtggt
     3241 tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga gttttgcaga
     3301 tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc gcaacagttt
     3361 ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc gctcaagata
     3421 aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca aatgtaataa
     3481 ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca aaagtgatgc
     3541 ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta cagttgaagt
     3601 cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc aattttgttt
     3661 cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa gtggagttag
     3721 agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta gcgaaaatca
     3781 aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt aatggttagc
     3841 aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata aatatggtag
     3901 ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc ggttcaatga
     3961 tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa ggtccagatg
     4021 acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac gaagtgttat
     4081 tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc
     4141 acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt gaagcctacg
     4201 catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt gaaacagcaa
     4261 aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg aaagcagcag
     4321 ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa
     4381 tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt gcgacagctt
     4441 ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta tcaaaggatg
     4501 atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca aatgttattc
     4561 ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc aaagcattgc
     4621 ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta gacatcaaag
     4681 aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt
     4741 ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg
     4801 atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg aaaaaagcaa
     4861 aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc ttatcatgtc
     4921 tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg aaggtattct
     4981 tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt tagttaccgg
     5041 taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt taggttgggc
     5101 aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat cagcaattat
     5161 tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc
     5221 tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt
     5281 tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat
     5341 cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt tgctatttat
     5401 cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa cagatggtat
     5461 ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca acatgatggc
     5521 tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt
     5581 aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact tgaacctttc
     5641 taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc ctttaggcga
     5701 cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa
     5761 ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca aggttcatgg
     5821 aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc aatcaaaaaa
     5881 ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga attctttaat
     5941 acacacccat acattgcttc tccaatttta ggggttacat tggctctgga agaagaacgt
     6001 gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg tatgatgggt
     6061 cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat gctaggtgca
     6121 ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt cttcttaggt
     6181 tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta caaagctggt
     6241 tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa aggtgcttca
     6301 attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat caaattctta
     6361 ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga taaattgcct
     6421 tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt agccctttct
     6481 cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg tttagctgca
     6541 ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc aatcgttatc
     6601 atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt ataatccata
     6661 aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt cataaacaaa
     6721 tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg taattgatgc
     6781 tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtaata atggttttga
     6841 gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag aagtggatta
     6901 tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca ttcaaacgaa
     6961 aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag
     7021 agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg tagtgaaacg
     7081 tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta agaggaggtt
     7141 ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat tatttgtccc
     7201 ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta tgagttggtt
     7261 atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg
     7321 aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa gtattggaca
     7381 agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc catctatttt
     7441 aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat cataagtttt
     7501 tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt ggctttattt
     7561 atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag
     7621 tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga tgctacgtgt
     7681 agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg ttaccagtcc
     7741 gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg ttattacttt
     7801 ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag cgcaggctca
     7861 agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa acttagtacg
     7921 caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt tagaatattc
     7981 ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga tcgaagaact
     8041 cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc atattgcgga
     8101 tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc
     8161 aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt ttgatacgat
     8221 cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga
     8281 acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg tttccgatat
     8341 gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt
     8401 cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt
     8461 agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat ttggacattc
     8521 tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt ctcgtgcaga
     8581 aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact taccaggaat
     8641 cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc gtgtgttgac
     8701 tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa
     8761 atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca ctatagaaga
     8821 acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata gcagtttgca
     8881 gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag agaattcgga
     8941 tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa aaatttgatt
     9001 ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt
     9061 atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac ttttgccagc
     9121 aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt agcaaacgag
     9181 gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc agttcctaaa
     9241 gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac ggatatcgat
     9301 ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga acaaccaaca
     9361 atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat caagacacga
     9421 gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag acctaacaaa
     9481 agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca acgaagatga
     9541 atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg caaaaaaaga
     9601 tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca atcggatctc
     9661 aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg tgacagatgt
     9721 accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta gtgatgaccg
     9781 ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag aagctggggc
     9841 gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg gaaaattcta
     9901 tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat tgattaacgg
     9961 aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga
    10021 taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag ccagccaaat
    10081 ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat tggtagagcg
    10141 ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat acaatcaaga
    10201 tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag atgaagaaaa
    10261 gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga tgaatttagg
    10321 cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca tcatgaaaat
    10381 gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg gacgaagcaa
    10441 tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg ttactgttgc
    10501 tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt tagtcgtagc
    10561 aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga
    10621 tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt
    10681 ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa tgactatcac
    10741 gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg aatagatggc
    10801 acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat ttgatgccga
    10861 tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt tacgtgccaa
    10921 tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta ttatgttcaa
    10981 aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg
    11041 gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc acatggagcc
    11101 tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc tgatgaagga
    11161 aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct
    11221 taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa gaagatatcc
    11281 aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag cggacaatgt
    11341 tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga tcatcggtcg
    11401 tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg ctcgggcgat
    11461 ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata ccacgatcgc
    11521 tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat tagctgttcc
    11581 tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc agcaggcgtt
    11641 tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt
    11701 tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg
    11761 attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga tcgcattaag
    11821 ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc
    11881 ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac agcagcagcg
    11941 ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca tgggaagagg
    12001 ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg
    12061 ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac aagagataga
    12121 caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa
    12181 aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta gattcgttta
    12241 aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca gaagagacct
    12301 tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac gaggaaagct
    12361 gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa agaaatcttg
    12421 tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag ttattcagtg
    12481 aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc ttcctttgat
    12541 gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt gcatacgtat
    12601 tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag aggaaaacct
    12661 accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg tttgcttaca
    12721 ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct tctgctgctt
    12781 cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc tgcagatatc
    12841 gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga aagaaagaca
    12901 gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca acctgaagaa
    12961 atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca gataagagat
    13021 gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg taaagatgaa
    13081 cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg tgaggcgttg
    13141 gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga cgttttttct
    13201 tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa atgagggaaa
    13261 gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt gaaacaagag
    13321 aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac gagcggtttg
    13381 ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc
    13441 cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt gatctatctg
    13501 ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact gatgcagctt
    13561 tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag ctagcttgga
    13621 aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac
    13681 ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct tttatttcac
    13741 taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa gtgattgctt
    13801 taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga attgttcgtg
    13861 accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca caaggtggat
    13921 atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat atcgagttct
    13981 tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt aatatggcag
    14041 aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact gtgaaaatca
    14101 agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc attgattaag
    14161 aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg tttgattact
    14221 cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat cctgaaaagc
    14281 aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact gaaactgatc
    14341 aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac aaacgaagat
    14401 gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga ccaaatggaa
    14461 aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa tttgattgat
    14521 aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt tttgatgatt
    14581 acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta cctttaagtt
    14641 gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt attccggaat
    14701 tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt gcgggtaaat
    14761 ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc gattatatac
    14821 gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa caagaaggat
    14881 tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg attgttcgac
    14941 gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt atcacattag
    15001 ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac gaacatcagg
    15061 aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat gtagctttcc
    15121 aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg gaaaaaaggg
    15181 tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg ctccatttcc
    15241 agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa ttaaaagaag
    15301 aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca ggttacggag
    15361 cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt tcagaactgc
    15421 aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc caaagtgctt
    15481 actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta ttggagttag
    15541 atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag ttgaaacgta
    15601 aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc gaagagctag
    15661 cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag
    15721 agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag
    15781 aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac actgagtttg
    15841 aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt gaccaaatcg
    15901 aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg
    15961 gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct gagcaaggtg
    16021 tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt gcacaggcca
    16081 tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc actcatctgc
    16141 ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg attgatggac
    16201 gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa attgcacgta
    16261 tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa ctattgaaac
    16321 tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca gctgatagct
    16381 atattttatt tttacatgac actcattaca gcagtcaata caacagatcc aatcgttaaa
    16441 atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt ttgttttttt
    16501 tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa tcttttttcc
    16561 atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata ggtagttacg
    16621 atacaaataa ataatgttaa tagaactagc catataaagg catcatccct ttcagcaata
    16681 ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt taaaataatc
    16741 gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat tgctgaaaaa
    16801 gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat tgcctgctct
    16861 gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg gaaaagttta
    16921 ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct gaaataaacg
    16981 aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga tacttgaagt
    17041 ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt gtaagagagt
    17101 gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga
    17161 aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc
    17221 ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta
    17281 ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat
    17341 tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct
    17401 gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca
    17461 caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat
    17521 gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg
    17581 gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata
    17641 aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt
    17701 tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt
    17761 tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa
    17821 agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac
    17881 agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat
    17941 tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac
    18001 ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat
    18061 actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc
    18121 agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa
    18181 aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggc
    18241 tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc
    18301 cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct
    18361 gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc
    18421 cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat
    18481 gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt
    18541 tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac
    18601 gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga
    18661 ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta
    18721 tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt
    18781 agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa
    18841 agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt
    18901 agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga
    18961 agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc
    19021 ctctcctcag ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga
    19081 tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta
    19141 ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc
    19201 tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga
    19261 aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag
    19321 aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt
    19381 agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc
    19441 attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc
    19501 accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa
    19561 accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt
    19621 acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa
    19681 tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg
    19741 attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga
    19801 tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg
    19861 ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac
    19921 aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac
    19981 tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg
    20041 acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa
    20101 agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga
    20161 gtattggatt gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg
    20221 ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa
    20281 aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct
    20341 cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg
    20401 ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca
    20461 aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat
    20521 ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga
    20581 aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg
    20641 gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag
    20701 ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga
    20761 ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa
    20821 aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa agttatttag
    20881 aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga
    20941 tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa
    21001 caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt
    21061 atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat
    21121 tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg
    21181 accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta
    21241 acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa
    21301 aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac
    21361 ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga
    21421 atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc
    21481 gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag
    21541 ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg
    21601 ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata tagtgtacct ggatacaatg
    21661 tatcggcaaa aaacggaaca gcccaaatcg cttctgatac agggggatat caaacaggag
    21721 atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct
    21781 tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa
    21841 atccattgat gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa
    21901 caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag
    21961 ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa
    22021 aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg
    22081 gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg
    22141 ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac
    22201 ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag
    22261 agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt
    22321 tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa
    22381 ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca
    22441 atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg
    22501 caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta
    22561 atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg
    22621 aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct
    22681 gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat
    22741 ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc
    22801 atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct
    22861 tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg
    22921 ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta
    22981 gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt
    23041 ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt
    23101 aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc
    23161 ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg
    23221 atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga
    23281 aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt
    23341 acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga
    23401 agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt
    23461 gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt
    23521 agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga
    23581 agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac
    23641 gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa
    23701 tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat
    23761 ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat
    23821 taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc
    23881 taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca
    23941 gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa
    24001 agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa
    24061 aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc
    24121 tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca
    24181 ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt
    24241 ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga
    24301 aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt
    24361 aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt
    24421 gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca
    24481 agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc
    24541 gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc
    24601 tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta
    24661 caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga
    24721 ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa
    24781 tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa
    24841 tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa
    24901 aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag
    24961 cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg
    25021 taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta
    25081 aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac
    25141 aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa
    25201 agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat
    25261 cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata
    25321 gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc
    25381 cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg
    25441 gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc
    25501 catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag
    25561 caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa
    25621 tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc
    25681 ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt
    25741 ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa
    25801 caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa
    25861 ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga
    25921 aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt
    25981 tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct
    26041 gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac
    26101 tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca
    26161 agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa
    26221 attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg
    26281 ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga
    26341 attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata
    26401 ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa
    26461 tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata
    26521 caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc
    26581 aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat
    26641 caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa
    26701 aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac
    26761 ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa
    26821 cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa
    26881 cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac
    26941 aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt
    27001 ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata
    27061 ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca
    27121 ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt
    27181 gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca
    27241 gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga
    27301 gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg
    27361 ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca
    27421 aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata
    27481 cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag
    27541 ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt
    27601 acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc
    27661 gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa
    27721 aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg
    27781 catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa
    27841 gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac
    27901 ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt
    27961 cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg
    28021 gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca
    28081 ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa
    28141 gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg
    28201 gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa
    28261 ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa
    28321 gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg
    28381 gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt
    28441 tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa
    28501 tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg
    28561 aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc
    28621 attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt
    28681 aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt
    28741 acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac
    28801 cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt
    28861 tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga
    28921 aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt
    28981 attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt
    29041 tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct
    29101 tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg
    29161 tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt
    29221 gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga
    29281 tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga
    29341 tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac
    29401 ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag
    29461 acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc
    29521 aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt
    29581 tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt
    29641 caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg
    29701 tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc
    29761 aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag
    29821 ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga
    29881 attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc
    29941 ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa
    30001 atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc
    30061 ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac
    30121 atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa
    30181 tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata
    30241 aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg
    30301 caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag
    30361 gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa
    30421 atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag
    30481 tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat
    30541 acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa
    30601 acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta
    30661 attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc
    30721 tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat
    30781 tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt
    30841 catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat
    30901 gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg
    30961 atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa
    31021 gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct
    31081 atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc
    31141 aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc
    31201 gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga
    31261 acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt
    31321 gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa
    31381 atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga
    31441 ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat
    31501 cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg
    31561 gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag
    31621 aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa
    31681 gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg
    31741 atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg
    31801 ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca
    31861 cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc
    31921 gactagacag tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga
    31981 tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg
    32041 aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg
    32101 ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa
    32161 aggtggttta ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa
    32221 aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta
    32281 tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa
    32341 acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga
    32401 tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc
    32461 acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac
    32521 tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct
    32581 tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt
    32641 gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc
    32701 attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct
    32761 tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac
    32821 ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta
    32881 gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga
    32941 cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc
    33001 agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg
    33061 ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac
    33121 aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa
    33181 attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga
    33241 aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca
    33301 tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag
    33361 taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg
    33421 atgggataca catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa
    33481 agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga
    33541 taagcaacga gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt
    33601 tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa
    33661 aggctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt
    33721 agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa
    33781 tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac
    33841 accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt
    33901 ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc
    33961 agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata
    34021 tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt
    34081 tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta
    34141 tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt
    34201 tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac
    34261 tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc
    34321 tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc
    34381 tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc
    34441 atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg
    34501 tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat
    34561 cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg
    34621 gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa
    34681 tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca
    34741 tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg
    34801 atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg
    34861 tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa
    34921 gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc
    34981 agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat
    35041 ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt
    35101 attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg
    35161 ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga
    35221 agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt
    35281 tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga
    35341 tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa
    35401 attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga
    35461 agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga
    35521 attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga
    35581 agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc
    35641 aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat
    35701 cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat
    35761 ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg
    35821 tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg
    35881 tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta
    35941 aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta
    36001 attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt
    36061 caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg
    36121 gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt
    36181 cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt
    36241 aagcttctgg gctattttca accatttctg cactatttct aaattccaat ttgattggtt
    36301 ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg
    36361 gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga
    36421 cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt
    36481 tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat
    36541 cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt
    36601 cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa
    36661 cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct
    36721 tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta
    36781 caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc
    36841 ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc
    36901 gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga
    36961 tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc
    37021 tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga
    37081 tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg
    37141 ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt
    37201 gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat
    37261 catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca
    37321 atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag
    37381 cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag
    37441 acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat
    37501 ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc
    37561 cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata
    37621 agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta
    37681 atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata
    37741 ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc
    37801 tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc
    37861 cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa
    37921 atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg
    37981 cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt
    38041 cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat
    38101 ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc
    38161 ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt
    38221 gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg
    38281 tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata
    38341 tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga
    38401 ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg
    38461 tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat
    38521 caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa
    38581 ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa
    38641 gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat
    38701 caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc
    38761 attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt
    38821 tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc
    38881 aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa
    38941 aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga
    39001 taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg
    39061 atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc
    39121 ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat
    39181 ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga
    39241 cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga
    39301 ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt
    39361 atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc
    39421 atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc
    39481 agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg
    39541 tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata caatagaaag
    39601 aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg
    39661 tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg
    39721 aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga
    39781 agactttttt caatattact catatggaca ccatttatat tgcttctccg aatagtcttc
    39841 actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag
    39901 ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt
    39961 atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact
    40021 taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct
    40081 atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg
    40141 cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa
    40201 aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga
    40261 tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt
    40321 taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg
    40381 aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg
    40441 cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag
    40501 atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag
    40561 tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt
    40621 tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa
    40681 agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca
    40741 aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct
    40801 actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga
    40861 actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt
    40921 ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc
    40981 agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa
    41041 agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa
    41101 cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt
    41161 ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga
    41221 gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg
    41281 tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca
    41341 tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta
    41401 tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc
    41461 gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag
    41521 aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga
    41581 agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac
    41641 actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat caaatataac
    41701 acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga
    41761 tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc
    41821 agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa
    41881 taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa
    41941 attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg
    42001 acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga
    42061 agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct
    42121 ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc
    42181 gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc
    42241 agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt
    42301 tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc
    42361 gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct
    42421 caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga
    42481 gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat
    42541 gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt
    42601 atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg
    42661 gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca
    42721 atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt
    42781 tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt
    42841 cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa
    42901 ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt
    42961 cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg
    43021 gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt
    43081 gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag
    43141 gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt
    43201 tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt
    43261 cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct
    43321 gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg
    43381 caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac
    43441 gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa
    43501 gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct
    43561 atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg
    43621 cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa
    43681 gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg
    43741 ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat
    43801 atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa
    43861 gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga
    43921 agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat
    43981 cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc
    44041 tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa
    44101 aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc
    44161 gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat
    44221 caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta
    44281 aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca
    44341 ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag
    44401 atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat
    44461 ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg
    44521 tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca
    44581 ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg
    44641 tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat
    44701 ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt
    44761 ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat
    44821 ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac
    44881 aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc
    44941 ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca
    45001 agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga
    45061 tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga
    45121 aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa
    45181 aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg
    45241 gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat
    45301 gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca
    45361 caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca
    45421 tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc
    45481 gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt
    45541 ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg
    45601 gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa
    45661 aacatttagt tgctttgcgt gatgctttta tcggcatact gcccgcaaca atggcgggat
    45721 cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct
    45781 atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa
    45841 tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt
    45901 gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt
    45961 tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga
    46021 atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag
    46081 atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg
    46141 cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc
    46201 ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg
    46261 cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag
    46321 gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat
    46381 tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat
    46441 tctggttctt tggtattcat ggatcaaatg tattggcacc agtgctagag ggaatctggg
    46501 gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta
    46561 caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt
    46621 tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct
    46681 tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta
    46741 tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt
    46801 ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc
    46861 ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt
    46921 cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg
    46981 tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag
    47041 gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg
    47101 aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc
    47161 aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa
    47221 ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca
    47281 aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt
    47341 tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt
    47401 agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg
    47461 tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg
    47521 aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata
    47581 ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag
    47641 cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct
    47701 cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt
    47761 ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg
    47821 attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt
    47881 tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa
    47941 tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg
    48001 aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct
    48061 cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt
    48121 atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc
    48181 tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga
    48241 tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg
    48301 acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca
    48361 aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag
    48421 cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc
    48481 atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc
    48541 tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga
    48601 acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa
    48661 attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac
    48721 gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt
    48781 ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga
    48841 aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc
    48901 atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt
    48961 tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga
    49021 tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca
    49081 tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac
    49141 gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt
    49201 gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa
    49261 tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt
    49321 catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt
    49381 ttgttttctt ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc
    49441 ttttcccgat tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta
    49501 tcattcattg gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt
    49561 aaggactagt ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg
    49621 caagtaaaaa acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg
    49681 atagagcaat cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtataa
    49741 ataacccctg tatcctttgc ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc
    49801 aaactagtta tcatttttat ataaactatc gaacacagta ttgatttttt ctttatcctg
    49861 atcttcaagc tctcgaacaa tatgaagata agtagacatt gtagtttcta aactgctatg
    49921 tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg
    49981 tgtatgcctt agtccatgca aagtaaattg cttttcaatc cctaattctt ttaatttttt
    50041 gcgcaaatat ttgctgacgg cgtttgatga aactaatcca ttttttaagt tgaaaaaaac
    50101 aaaattatga gggtttttga tttcaaaatt ctcgaataat tctttttgat tgattttaaa
    50161 cttttttaag agatcaatcg tatgtttatc aatggaaat
//
LOCUS       DAZUKA010000004        50078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000004 DAZUKA010000000
VERSION     DAZUKA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.004"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            452..1180
                     /locus_tag="ACG8PN_000173"
     CDS             452..1180
                     /locus_tag="ACG8PN_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4860359.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1198..2391
                     /locus_tag="ACG8PN_000174"
     CDS             1198..2391
                     /locus_tag="ACG8PN_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4860360.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2384..3379
                     /gene="lacD"
                     /locus_tag="ACG8PN_000175"
     CDS             2384..3379
                     /gene="lacD"
                     /locus_tag="ACG8PN_000175"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4860361.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3376..4317
                     /locus_tag="ACG8PN_000176"
     CDS             3376..4317
                     /locus_tag="ACG8PN_000176"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS4860362.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4363..6129
                     /locus_tag="ACG8PN_000177"
     CDS             4363..6129
                     /locus_tag="ACG8PN_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFS4860363.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6146..6625
                     /locus_tag="ACG8PN_000178"
     CDS             6146..6625
                     /locus_tag="ACG8PN_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4860364.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6638..7528
                     /locus_tag="ACG8PN_000179"
     CDS             6638..7528
                     /locus_tag="ACG8PN_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4860365.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7515..8324
                     /locus_tag="ACG8PN_000180"
     CDS             7515..8324
                     /locus_tag="ACG8PN_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4860366.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8340..8744
                     /locus_tag="ACG8PN_000181"
     CDS             8340..8744
                     /locus_tag="ACG8PN_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4860367.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(8925..9863)
                     /gene="yidC"
                     /locus_tag="ACG8PN_000182"
     CDS             complement(8925..9863)
                     /gene="yidC"
                     /locus_tag="ACG8PN_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4860368.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10197..11279)
                     /locus_tag="ACG8PN_000183"
     CDS             complement(10197..11279)
                     /locus_tag="ACG8PN_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4860369.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11421..12872
                     /locus_tag="ACG8PN_000184"
     CDS             11421..12872
                     /locus_tag="ACG8PN_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4860370.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12865..13587
                     /locus_tag="ACG8PN_000185"
     CDS             12865..13587
                     /locus_tag="ACG8PN_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4860371.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13607..14758
                     /locus_tag="ACG8PN_000186"
     CDS             13607..14758
                     /locus_tag="ACG8PN_000186"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4860372.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15106..15381)
                     /locus_tag="ACG8PN_000187"
     CDS             complement(15106..15381)
                     /locus_tag="ACG8PN_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4860373.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15489..16253
                     /locus_tag="ACG8PN_000188"
     CDS             15489..16253
                     /locus_tag="ACG8PN_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4860374.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16377..16880
                     /locus_tag="ACG8PN_000189"
     CDS             16377..16880
                     /locus_tag="ACG8PN_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4860375.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17145..17792
                     /locus_tag="ACG8PN_000190"
     CDS             17145..17792
                     /locus_tag="ACG8PN_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4860376.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      17835..17902
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            17953..19221
                     /locus_tag="ACG8PN_000191"
     CDS             17953..19221
                     /locus_tag="ACG8PN_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4860377.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19398..20600)
                     /locus_tag="ACG8PN_000192"
     CDS             complement(19398..20600)
                     /locus_tag="ACG8PN_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4860378.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21127..21852
                     /locus_tag="ACG8PN_000193"
     CDS             21127..21852
                     /locus_tag="ACG8PN_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFS4860379.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21842..22516
                     /locus_tag="ACG8PN_000194"
     CDS             21842..22516
                     /locus_tag="ACG8PN_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFS4860380.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22804..23088)
                     /locus_tag="ACG8PN_000195"
     CDS             complement(22804..23088)
                     /locus_tag="ACG8PN_000195"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFS4860381.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23382..24689)
                     /locus_tag="ACG8PN_000196"
     CDS             complement(23382..24689)
                     /locus_tag="ACG8PN_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4860382.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25178..26284
                     /locus_tag="ACG8PN_000197"
     CDS             25178..26284
                     /locus_tag="ACG8PN_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860383.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            26875..27075
                     /locus_tag="ACG8PN_000198"
     CDS             26875..27075
                     /locus_tag="ACG8PN_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4860384.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            27222..30011
                     /locus_tag="ACG8PN_000199"
     CDS             27222..30011
                     /locus_tag="ACG8PN_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4860385.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            30058..30585
                     /locus_tag="ACG8PN_000200"
     CDS             30058..30585
                     /locus_tag="ACG8PN_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4860386.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            30606..31796
                     /locus_tag="ACG8PN_000201"
     CDS             30606..31796
                     /locus_tag="ACG8PN_000201"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4860387.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            31836..33134
                     /gene="asnS"
                     /locus_tag="ACG8PN_000202"
     CDS             31836..33134
                     /gene="asnS"
                     /locus_tag="ACG8PN_000202"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4860388.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            34068..34742
                     /locus_tag="ACG8PN_000203"
     CDS             34068..34742
                     /locus_tag="ACG8PN_000203"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4860389.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            34739..35080
                     /locus_tag="ACG8PN_000204"
     CDS             34739..35080
                     /locus_tag="ACG8PN_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860390.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            35110..35469
                     /locus_tag="ACG8PN_000205"
     CDS             35110..35469
                     /locus_tag="ACG8PN_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4860391.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            35993..38074
                     /locus_tag="ACG8PN_000206"
     CDS             35993..38074
                     /locus_tag="ACG8PN_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4860392.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            38385..39851
                     /locus_tag="ACG8PN_000207"
     CDS             38385..39851
                     /locus_tag="ACG8PN_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4860393.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            40136..40634
                     /locus_tag="ACG8PN_000208"
                     /pseudo
     CDS             40136..40634
                     /locus_tag="ACG8PN_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            40708..41148
                     /locus_tag="ACG8PN_000209"
     CDS             40708..41148
                     /locus_tag="ACG8PN_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4860394.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            41161..41370
                     /gene="copZ"
                     /locus_tag="ACG8PN_000210"
     CDS             41161..41370
                     /gene="copZ"
                     /locus_tag="ACG8PN_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4860395.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            41370..43556
                     /locus_tag="ACG8PN_000211"
     CDS             41370..43556
                     /locus_tag="ACG8PN_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4860396.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            43576..45747
                     /gene="copB"
                     /locus_tag="ACG8PN_000212"
     CDS             43576..45747
                     /gene="copB"
                     /locus_tag="ACG8PN_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4860397.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(45859..48030)
                     /locus_tag="ACG8PN_000213"
                     /pseudo
     CDS             complement(45859..48030)
                     /locus_tag="ACG8PN_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(48020..48772)
                     /locus_tag="ACG8PN_000214"
     CDS             complement(48020..48772)
                     /locus_tag="ACG8PN_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4860398.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(48769..49092)
                     /locus_tag="ACG8PN_000215"
     CDS             complement(48769..49092)
                     /locus_tag="ACG8PN_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860399.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(49106..49981)
                     /locus_tag="ACG8PN_000216"
     CDS             complement(49106..49981)
                     /locus_tag="ACG8PN_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4860400.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
BASE COUNT        16295 a         8768 c        10592 g        14423 t
ORIGIN      
        1 tacgccataa ggaaactaga aaaagacaga attatcataa aaaatttttg ttatttttaa
       61 aaatgtacaa caaaatttcg actatcagac cggcatgaga gaaaagttat ctaaaaaata
      121 aaaaatccta ctttatttag agaaagccat ttttatttac atagtagtct attaggccca
      181 tttgcttgtc gatagtagaa atagccacag atgtataaag aatatttttt ctttacttct
      241 ctagaccatt tagctgaatc tttgccaaga ttcagctttt ttttgttttt tatttgtcaa
      301 ctggtatttt tgaggtatct tttgatacca gttctaaatt accttgaaac cttgttataa
      361 tagctttttt acgccaaaaa ccctttgaca aatgaggaat atgtgggtat tcttgtgttg
      421 tggttataac cagtaaagaa gaggtatttt tatggagagc tcaagtaaag gaaaatcatt
      481 atattatcaa ctagttgacg tattgaaaga acgtatcgaa acacagatga cacctcatga
      541 caaattgtta tctgaaagag aactttgtca gcattatgga gtgagtcgga caacggttcg
      601 ccttgctttg caagaattag agacgcaagg gtatatttat cgccgacacg gaaaaggaac
      661 atatgtgtct gatctatcag ataagtcgtc tgatctggcg ggagcgtata gttttacaga
      721 agagatgagg gaattgggac gggtgcctaa tacagtcatc ttggaattca agcaaatgga
      781 agcaaacaaa aaggttgcca agaatctaaa catcaattta ggcgaaacag ttttcaaatt
      841 gaaaagactt cgtttagcgg atgacgaacc attgatggtg gaacgttctt atttacctgc
      901 aaaactattt attggtcttg atctgccatt acttgaaagt aagccattgt atgatgtgtt
      961 ttctcaggat tatgaacagc aagtccaatt agcagatgaa gaattttatg caagtatcgt
     1021 tcaaaagaaa gatgctgatc cgcttcgtat cgctgatggt gccccggtat tatatctaag
     1081 ccgaacgacc tataatgtga aaaatgaaat tattgaatat acggatagtg tagcgagagc
     1141 agaccagttt cattataaga tccgccacgt ccgaaaatga ttgagaggga aaacaacatg
     1201 tttgaactat cgaaggataa attaaaagag ttgggtgcgg aaataactac gcgagaaatc
     1261 aaacagcaac cgcaattatg gaaagaaaca gctgagatat tagcacagaa tcaagaaaaa
     1321 ttagcattgt tttttgacca gataaaaaaa gcagcaaatg gcaaaagagt tcgagtgatt
     1381 tttactggtg caggcacatc acaatatgtc ggggatacag cagtacctta tttaaatgaa
     1441 tacggtgata acgatcactt tatttttcat agctttggta cgacagatat cgtgtcttca
     1501 ccaaaagaat ttctatttcc ggatgaaccg acattactgg tgtcatttgc acgtagtgga
     1561 aatagtccag aaagtgtggc aacagtcaag attgccaatg aacaaatcaa gacattgttt
     1621 catttgacga ttacttgtgc taaagaaggt cagttagcaa agaatgcagc gacagatgaa
     1681 cgaaattatc ttttactgat gccagaaaaa tcaaatgatg caggatttgc catgacagga
     1741 agttttacgt gcatgttgtt gagtgcgtta tgggtattcg accaaactgc cgttgagaaa
     1801 aaagaaagtt atcttcaaca attgatcaga actggagaag atctaatttc acgagatcaa
     1861 gaaatccaaa aattattgcc taagtcattt gaacgtatcg cctatcttgg ttcaggaagt
     1921 ttggctggtg caacgagaga agcacaattg aaaatattgg aattgacagc cggtaaagtg
     1981 gcaacgatct ttgattcttc tatgggcttc cgccatggac caaaatcgtt tttaaatagc
     2041 gaaacacttg ttattggttt tgttaataac caaccgtata caagaaatta tgacttagat
     2101 attctagaag aaataaaaga tgaccaaatt accacgagtg tcatcgcgat cgcccaacca
     2161 ggaaacatcg atttcacagg tacaagtctt ttgttagaag cttcgatcag cctacctgat
     2221 gcgtatcttg ccttacctat gatcatgaca gcccaaatca ttgcgctcga tacgtcgata
     2281 aaagtagcga ataaaccaga tacaccatcg cctacaggta cagtcaatcg agtggtcaaa
     2341 ggtgtcatta tccatgaata tgtgaacaaa ggggaaacga aggatgaatg aagaaaaaat
     2401 tgcagctatg aagagtttat caacaaaaga aggaatcatc agtgcattgg caattgatca
     2461 acgcggtgct ttgaaaaaga tgatcaaagc gttaggaaaa gaaccaacgg cgcaacagat
     2521 cactgaattc aaagaattag tatcagcaga attaacgcct tatgcttctg ctattttact
     2581 agatccagag tatggattgc cagctgctaa agccagacat caggaagcag gactactttt
     2641 agcctatgaa aaaacgggtt atgatgcgac aactccagga cgtctgcctg atttattagc
     2701 ggattggtct acgcttagat taaaggaaca aacagccgat gcgatcaagt ttttgcttta
     2761 ttatgatgta gatgaagacc cagagatcaa tcatttgaaa catgtattta tcgaacgttt
     2821 aggcagtgaa tgtgcaggtg aaaagcgtcc gttttatcta gaattggttt cgtatgatgc
     2881 caaaaccaat gacagcactt cattagaata tgcgaagatc aaaccgcata aagtgatcga
     2941 gatgatgaaa gaattttctg atcctaaata taaagtggat gtattgaaag tagaagtgcc
     3001 agtcaatatg aactatgtag aaggttattc tgatggtgaa attgcgtata gtaaaaaaga
     3061 agcagcggca ttcttcaagg aacaaagtga tgcaacaccg ttaccattta tcttcttgag
     3121 tgcaggggtt tcgactgcat tgttccaaca aacattgatc tttgctcatg aatctggttc
     3181 aacattcaac ggtgtgttat gcggacgtgc gacttggaaa gatggagtca aagcatatgt
     3241 tgaaaatggt agagaaggtg cgattgagtg gttgagaaca caagggcgac aaaatatcga
     3301 atcgttgaat aaagtgataa atgaaaacgc caccccttgg tttgacaaag taaaagaaga
     3361 ttcggtgaca gcaaaatgat cacgacagtt accatgaatc cttcaattga catcgcttac
     3421 acgttggatg aattacggat cgaccaagtc aatcgggtca aaacggtcaa caaaacggct
     3481 ggaggaaaag gcttgaatgt tgctcgtgtc cttcatttat tagcagatga tgttttagct
     3541 actggattag tcggcgggca gcatggagca tatatcaaag aacaactcga tcaatcaggg
     3601 ataacgcatc aatttttacc tatcaaaggg gaaacgagag attcgattgc tattttacat
     3661 ggagtgaatc aaacagaact attggaaagc ggtcctgttg tgacggaaga agagcaacat
     3721 aaatttaccg agcattttgc acgactaatg gaaacaactg atatctttac gatttcgggt
     3781 agtctgcctc aaggattttc taaaagttat tatgcagagt tacttgccct aacgaataaa
     3841 aaaggaacac gagtattgtt agatacttct ggtcaaacct tgcttgaagt cttggaaggc
     3901 aaggaaaaac catttttgat caaacctaat ttggaagaat taggagagtt ggtccaccaa
     3961 acaatttctt cggctacttc gttagatgaa atccaatcat tattggatca tccgcttttt
     4021 gaaggaatca attggatcgt tctttcgcta ggcgcaaaag gagcaatcgc taaagtaggg
     4081 agacacttct atcaggttac gattcctaaa atcgcggtgg tgaatcctgt ggggtcagga
     4141 gattcgacta ttgcaggctt agcacatgct ctatcaaaga gctatacgat tgaagaaaca
     4201 cttgcgtttg cgatggcctg tgggatggcg aatgcacaac aagaaaaaac aggatggatc
     4261 gatcctgtga aagtcgatta ttttaggggg gaaatcaacg tcagaaagat tcgttgatta
     4321 taaaattaga aagcggtttc taaaaacgag gaggaaattt gcttgactag atttgaaata
     4381 aaagaagagt ttttgttaga cggtcaacct ttcaaaattt tatctggtgc catccattat
     4441 ttccgagtgc atccgacgaa ttgggagcgg tcattgcaca atttgaaagc gcttggattt
     4501 aatactgtgg aaacctatgt gccttggaat ctccatgaaa aagaagaagg aacctatgat
     4561 ttttcgaaaa tattggatct ttcccatttt ctagagttag cgcaatccct tggattatac
     4621 gcaatcgtac gtccagcccc ttatatttgt gcagaatggg aatttggtgg cttgcctgct
     4681 tggctattga caaaggagtg ccgtattcgt tcagcggatc ctcgcttcat gaagtatgtc
     4741 aaacggtatt accaaacgct actacctaaa atcgttcctc atctagtcac tcatggtggc
     4801 aacgtcttaa tggtgcaggt agaaaatgag tacggttcgt atggtgaaga gaaagcatac
     4861 ctgcgccaag taaaaaaata cttggaggaa ggtggaatcg atgtaccgct attcacttct
     4921 gatggacctt ggcaagccac attaagagca ggcattttga ttgacgatga tgtttttacg
     4981 acagggaatt ttggctcaaa agcaaaagaa aattttgcag atatggccca gttttttgaa
     5041 gcacatggta aaaaatggcc gttgatgtgt atggaatttt gggatggctg gtttaatcgt
     5101 tggaaagagc cgatcattaa aagagatccg gaagaattag cggatgcagt caaagaagca
     5161 ttacagatcg gcagtatcaa tctctatatg ttccatggcg gaaccaattt tgggtttatg
     5221 aatggctgtt cagccagagg cgcgattgat ttgccgcaag tcacttccta tgattacgat
     5281 gcaccattgg atgagcaggg aaatccaacg gaaaaatatt ttgctctgaa acgaatgatg
     5341 gcagaaaatt ttccagaaat gcagcaaatg gaaccatgga taaaagaaac aatcgtcaaa
     5401 aaaggaatca acttgaaaga aaaagtcagc tttttcgcaa cactggatac catcagcaag
     5461 ccgatccact cgacctatcc tcaaacaatg gaagaattgg gtcagaatac tgggtaccta
     5521 ttgtatcgaa caacgatcaa aaaagatgcg gtcaatgaaa aattacggat catcgacgga
     5581 cgtgatcggg tccatgtttt tctcgatgaa gaaaagcaag cgattcaata ccaaacagag
     5641 atcggtgaga ccattccatt gacacttgag aaagaagacc atcaaattga tctattattt
     5701 gaaaatatgg gacgggtcaa ctatggacac aagctgctag ctgatacgca aagaaaagga
     5761 atacgtacag gagtgatgag cgatcttcat tttatcacag aatggacaca gtattgttta
     5821 cctttagaga ctacagaaca catcgatttt tcaaaaaaat gggaagcagg tcaaccagca
     5881 ttttaccgct ttgaagcgga actgtcagaa atcggtgata cttatttgga ccttagtgag
     5941 tttgggaaag ggatcgtttg ggtgaatggc acgaatattg gtcgtttttg ggaagtagga
     6001 ccaattcttt cactgtttgt tcccgagggt ttatttagaa aaggacaaaa tgaaatcgtt
     6061 atctttgaaa cagaaggtag attcagtgaa gttatagatt ttgtcaaaga accaatagaa
     6121 aaaagttaga aaaggagaat ggatgatggc aattattgga gtaagaattg atggacgttt
     6181 gatacatgga caagtggcaa atctttggac gacaaagttg aatattggtc ggatcatggt
     6241 cgtagacgat gaggtagcaa caaatgcgat cgaaaaaagt gggttaaagt tggcaacgcc
     6301 agctggagtc aaattaagta ttttacctat tgaaaaagca gccgcaaata ttttagcagg
     6361 taaatatgat tctcaacgag tgctgatcgt agcgaaaaaa ccagatcgta tcttagcgct
     6421 agtagaaaaa ggtgtaccga tcaaagaatt aaatgtcggc aatatgtcac aaacagatga
     6481 aacacgttcc atcacaaaat caatcaatgt cgtagatgcg gatgtagagg tcttcaagaa
     6541 attagatcag cttggcgttc atttgatcgc tcaaatggtg ccaaatgaca aagcagaaga
     6601 ctttatgagt cttttaatca aataggagga atgattcatg gatattcaat ggtggcagat
     6661 attattatta acattatatg cagggtatca aattttagat gaattacaga tttattcttc
     6721 aatgagcgcc ccagtttttg caggattata tgctgggtta gtaatgggtg atttaaaagc
     6781 aggtttaatc attggtggaa gtatgcagtt gactgtttta ggggtaggga catttggtgg
     6841 tgcatcaaaa atcgatgcca actctggaac gatcttagca acagctttct ccatttctct
     6901 agggatgaac ccagaacaag cgattgcggc catcgcggtt cctgtagcaa gtttgatgat
     6961 ccaattagat attttggcac gttttgcgaa tacgtatttt gcacatcgga tcgatcaaca
     7021 tgtggaaaca atgaattaca aagcaattga acggaacttc ttgatggggg cattaccttg
     7081 ggcactttcc agaatggttc ctgtattctt ggccctagca ttcggtggtg gcttagtcca
     7141 aaatgttgtg gctgttttaa acggcgacct aaaatggcta ggagatggct tatctgttgc
     7201 aggggcagtc ttaccagcag ttgggtttgc aattttacta cgctacctac cagtaaagaa
     7261 acacatttct tatctgattt taggatttac gatcacagcg ttattagcaa cagtgttctc
     7321 gaatgtccaa ttgttaggaa caagtgtggc tagcgtggtc aaagatttcc caggaacatt
     7381 caattcatta ccgatgcttg ctgtggcatt gattggtcta gcactggcaa cgatcagtta
     7441 taagaatgat caaaagaatg accaacatcc agtagtaagt cagcaatcac aatctgatga
     7501 gggggaaatc gaagatgacg aaatctaatt ataaattgac caaagaagat tttaagcaga
     7561 tcaatcgtag aagtttgttt actttccaat taggttggaa ctatgaaaga atgcaaggtt
     7621 caggttatct gtatactatc ttgccacaac ttagaaaaat ctatggtgat gatacaccag
     7681 aattaaaaga aatgatgaaa acacatacgc aattttttaa tacgtctaat ttctttaaca
     7741 cgatcattac tgggatcgat cttgctatcg aagaaaaaga aggagtctct tcaaaggaaa
     7801 ctgtttctgg gatgaaagca ggattgatgg gttccttcgc tgcaattggt gattctgtat
     7861 ttgcggcatt gatcccgacc atatttggag ctttagccgc aaatatggca acagatggta
     7921 atccagtagg tatccttatc tggatccttg ctcaggtcgc tgtcatggtc tttcgttgga
     7981 aacaactaga atttgcctac aaagaaggga tttctctagt taccacgatg caatcacgct
     8041 tgactgcatt gacagatgcg gctactttac taggagtttt catggttgga gcgttagtag
     8101 caaccatgat caatgttaaa ttctcatggg caccaacgat tggggatgta acgttgaata
     8161 tgcaaaacta tatcgatatg atcttaccta aacttttacc agctggaatc gttggtggcg
     8221 tttattggat gttaggtaag aaaaatatga cacctactcg agcaatattc atcgtattga
     8281 tcgtatgtgt agcattatct gcattaggtg tgatttcgaa ataagggaga attggaaaga
     8341 tgagtagaca actagtcttg attagtcatg gtaaattttg tgaagaacta aaaaacagta
     8401 cagaaatgat catgggacca caagaaaata tctatacgat ccctttattg cctgaagatg
     8461 gcgcggacac gtataaagaa agattcctct ccgttacagc accattgaaa gattacgtag
     8521 tgctttgcga tctgttgggt gggacacctg caaataccgt ttccaagcta atcatggaag
     8581 gtgaaacgat cgaactatat gctggcatga atttgccgat ggtcatcgaa ttcatcaatt
     8641 cgcaaatgat tggagcagaa ccagatttta ttggttcggg tcgtgagagt atcgtttcag
     8701 tgaaccaagt gattgctggc atgggagacg atgaagaaga ataatcaagc acaacttctt
     8761 gactggtgat acaactggat ataaatacgg atgtataaag aggccgtgta taaaaaagcc
     8821 aacgtaccgc gaaatatagt tttctatatt tcacagtacg ttggctttta tcgtcataaa
     8881 atcagaaatg atctgggtcg atcgaagaag ctagttacaa ataactattt acgcttttgc
     8941 tgtttaccag cattccgacc attcttcgca ttgttttttg gtgcttgtac ttgttttttt
     9001 gtttgcttcg caggttcagc aggtgtgaca tctttacgag gcgtcaccac ttgttttggc
     9061 ggattttgtt ttaattcttc cgcaacttgc gcttttaatc ttggtttcat gatgacattc
     9121 gtaatgaatg tttgtagaca actaaagatc ccaccgacta cccagtaaag agtgacacca
     9181 gctggggcac tgatcgacat gaagacgatc atagcgggtg atacgatcaa catcgtacgc
     9241 atcgttttct tttgttcttc agggataccg atagttgaga tatagccttg aagtaaataa
     9301 gcaataccgg caatcgctac taaaactaag ctagggttac ctaggtcgat cccgtaaaag
     9361 gttgattcac ggatgccttc tgtatagcga gcagtgaaat atagcgcaga aaagatcggc
     9421 atttggatca gaagcggtaa acaaccgata ccaccagtca tacttaagcc attttcacga
     9481 taaacttgtt gcatttcagc ttgtgcagcc atttgttctt ctcgagttgt cgcagctttc
     9541 aatttttgtt gcgtagcttc aacttgtggt ttcaatgctt gcatcttttc agattggatc
     9601 attgtttttt tcgattgact aatacctaat ggcaaaatga tgatacgaac gatgactgtc
     9661 atcaagataa cagcccagcc gtaggaccaa ttaaagtttt ccactaaata tgtaatcgct
     9721 tgtcccattg ggtgaaccaa tatacggtaa atgattcctg aagtgtcagg gttaccattt
     9781 gaatccatgc ggacacaacc ggacaagaaa agcatgatcg tgacaagccc agagcccaat
     9841 aaccaatttt tccatttatt cataaaatac ttcctctttt ataattttta cacaagctta
     9901 actatactaa aaaagcgctc ataattcaat caacttcata gaaatttcat agaaataacc
     9961 gattggagga tcaaaaaaga cgcttaatgt taaaaagaag aatccagcta aaaatacttc
    10021 agtataaagc atacttagca cctccgttct ctaaaataag atgagcaacg gatatttcct
    10081 tgaaatcata attttagcgc aaaaaaaaga agtggaatgt caattttttc cacttctttt
    10141 tttttaccat tgagccgatc cataaaaaag ctgactgcca tggtataagg ggctaactat
    10201 ccctgtgtca cttgttctaa ctctttcatt ttctggacaa ccagtgctgg tgtgatcgtt
    10261 tctttcatga agtggatcat ttcatcaggt aaacatgcac gttcagctac ggcttgatag
    10321 tcactttctg ttaaatgtaa acccatatca gctaaactcg ttggtaagtt caagtgatga
    10381 taaaacggca gtaggtgttc gatttctgct aatttatttt cgatcatcag ctggacaagg
    10441 ataccgtatg caactttgtt tccatgaagt tgctgatggc tatcagggag tacggtcatc
    10501 gcatcgtgaa tggaatgagc tccagctgtt cggccgtagt catcgccaaa cccaccaacc
    10561 ataccagcca ataaaatgtt tgtttccacg acatcgataa aagcttgatt gattttttgc
    10621 tggtccatcg cttccattgc ctctgcactt ttctctaaaa ggatttcgcg gcatcgtaac
    10681 gctgcaaatt ccgctacttg gacttcgata ggcttgatag ttaactgaga gatgattgcg
    10741 tgagattcat accattttgc taaggtgtca ccaataccgg caatcatcat ctcgcgaggg
    10801 gagtgcaaaa tgatctttgg atcgatcaac actaaatcat ttgctttagg gaacatatca
    10861 tagcgaagca tcactccttc ttcattatag accacactaa gtggagtata agctgcgcac
    10921 gtcgcagcta aggtaggtaa gatgatgatt ggtaagtttg tcttataggc aatcaatttt
    10981 cctagatctg ctactttccc gccgccgact gcaataatac cattgaaatc cttctttttg
    11041 cataaagaaa caaaactgtc agcaagtgga tcggtacatt ctccaccata atattcaaaa
    11101 atttttgtta ttgataaatc tggaaaaaca tcttttactg ctttccaaga tgtttttcca
    11161 tggagaatca aaacgttgtc aatatgcctt ttgtaaaggt gttcagggag tgaatcccat
    11221 gcgttttctt ttatttcata ctcttgaggg gcaccgcgaa caattaaatt ttttgtcata
    11281 taaaaaactt cttccattat ttttttaaat taatcttttt ttattataat agttatgcta
    11341 gtatattagc aagttattta tgaatattta ttcttttata gcttttctga ttacattatt
    11401 attcaaaggt ggccaaatgg atgaaaaaag gaataaacat attattagtg gcgctgatgt
    11461 ttctactggt ttttttacca gtaaaagtac aaggtgcaga aaaagatgaa atttacgagc
    11521 ggataaaaca aagtggagaa ttagtcgttg gcctatctgc agactatgcg ccctatgaat
    11581 tccatgcgaa tgtggatggt aaggataaaa tcgttggttt tgatgtatcc attgccgaaa
    11641 agatcgcaaa ggatatgggt gtgaaactaa aagtagaaga acttggtttt gatgcattat
    11701 taggtgcgtt gaaaactggc aagatcgatt tagttatatc tggtatggcg gtgactgaag
    11761 aacgtttaca agaagttgac ttttctgacc catatttcgt ggtacagcaa aaagtactcg
    11821 ttcgtaaaga ggacaaaaat aaatttaaga cgacagccga cttttctggc ttagccgttg
    11881 gggcgcaaaa gcaaaccaca caagaggagt tagtgaaaaa tgaactgact ggtgcagagt
    11941 tgacctccct gcaaaaagtt cctgatctga tcaataattt gctgaatgaa aaagttgaag
    12001 gtgttgtgct ggaacaacca gttgcagaag catatgccca acagaataag aaagtagctt
    12061 tgactccagt aaaatttgcg caaggcgaaa aaaccacagc tattgcgatg tcaaaaaata
    12121 caccagaact gaaaaatcat gtcaatgcgt caatcaaaac gatcaatgac gacggcttgc
    12181 tgaaagatta tcaaaaacaa gcgaatcaat tgatgttcca agatgatcag tcctttcttc
    12241 aaaaatacgg tccgtattat ttgattggaa caaaatacac tattttatta gcttttgtgg
    12301 gtgtattgtt tggaatggtc tttggtagtt tgcttgcact gatgaaattg ggtaagaata
    12361 aatttttaaa ggcattagcc attatttata tcgaatacgt acggggaaca cctctcttag
    12421 ttcaaatctt tatcgtttac ttcggaacag gagtactggg attggatcta tcgaaactgg
    12481 tagcaggttg tattgctgtc tcgttgaata gtgctgctta tgtagccgaa attgtccgtg
    12541 ccgggatcaa tgcggtcaac aaaggacaga tggaagctgc acgttcttta ggaatggacc
    12601 agaaagaagc catgcgtttc atcatcttcc cacaagcaat caaaaatatt cttccagctc
    12661 taggaaatga gtttgtcacg atcatcaaag agtcctcagt tgtttcggtc attggggtat
    12721 cggaattgat ctttcaaacg ggcgtggtac aaggtgctag cttcaaacca ttcctaccat
    12781 atgtgttggt ctctttgatt tatttcgtgt tgaccttcag tatttcccgt atattagctt
    12841 tagttgaaag gagaatgggt ctgaatgctt aatgtaaaaa aactgtcaaa aagttttggc
    12901 gagaatgaag tcttaaaatc aattgatctt tctgtgaaag aaggagaagt agtggtgatc
    12961 attggccctt caggaagtgg gaaaagtaca attctacgtt gtatgaatct gctggaagaa
    13021 ccaacttctg gtgaaatctt ttttaaggga caaaacatta cagcccctga cagcgatatc
    13081 aatcaaattc gtcaaaaaat gggaatggta tttcaaagtt ttaatttgtt tccccatatg
    13141 aatgtactcg aaaatttgac catcacacct gtaaaaatca aaaaagaaga ccctaaaaaa
    13201 gcaaaagaac atgctctatc attattgaac caagttggtc taaaggaaaa agtggacagt
    13261 tatcctacca gtttatctgg gggacagcaa caacgggtag caattgctcg agcattagca
    13321 atgaatcctg atgtgatgct tttcgatgaa cctacatctg cacttgatcc tgaaatggtc
    13381 ggtgaagtgt tggcagtcat gcaggattta gctaaaaaag ggatgacgat ggtcgtggtc
    13441 actcatgaaa tgggttttgc taaagaagtt gccgatcgtg tgatttttat ggctgacggc
    13501 atcattcaag aagagggaac accgaaagag atttttgaaa atccgcaaaa tagtcgaaca
    13561 caggatttct tgaacaaagt gttgtaacaa agatggagga agtaagatgg acaaaagtaa
    13621 gattgcacgc cggtttcagc aaccagcaga aaatctgttg atgaatattg ctattttggc
    13681 aaaggaaaaa gaggatatta tcgatttatc gattggtgat cctgacttga tcactgatcc
    13741 gacgattatt gaagcagctt atacagatgt aaaaaatggt gctactaatt atactgcgcc
    13801 tgatggcagc aaagaattct tgtcaacggt cgtagatttt tatcaaaaga aatatggatt
    13861 gagcgttgaa tgcaaccaag ttcgagctac agtcggagca atgcacggga tgtatctagt
    13921 tttacaggca ttactagatc cagaggatga ggtcatcatc catgaacctt atttttcacc
    13981 atatcgggaa caagtcattt tatctggagg caagcctgtt tttgttccta cctatgaaga
    14041 aaagggcttc cagttggatg tggaagattt aaaagcagcg attacggatc gtacaaaggt
    14101 gatcgtcatc aattcgccga ataatcctac gggagctgtt ttttctgaaa gcacgttgaa
    14161 aaaaatcgct gaagtcgcaa tcgaaaatga actctatatc atttccgatg agatttatga
    14221 agactttgcc tttaaagaga agttcgtacc gatggcgcga tttgcgcccg ataatacgat
    14281 cactgtcagt ggtgtatcga aggggtttgc gatgactggc tggcgcttag ggtacatgat
    14341 cgcaccagca tacatcaatc aaattgctgg gttgatcaat gaaaacatca cttattccgc
    14401 accgactgct tctcaaagag gtgcaatcta cgccttaaag cactatgaac agcttgtacc
    14461 gccagttgtt gaagtattca aagaacgatt agcttatatt gagaaaagag tggcagaaat
    14521 cccttactta tctttatctt ctgtttctgg aagcatgtat gcttttatta atatcagtaa
    14581 gagtgggctc gattcagtaa cgtttgtgga agaggtttta gaaaaaagcg gcgtactttt
    14641 tgtccctgga aaagcgtttg gggaaacgat cggggacaac tatattcgac tagcagcgac
    14701 tcagccattg gaaatcttag gtgaagcctt tgatcgttta gcaaaactaa atttttagat
    14761 aaaactgtat atttgaaaac agctatgggt atccattaga acaaagaggg aaaatgatcc
    14821 tttcgagagt tggcttgttg actgggcagc gtcaggattt gacaatgaaa aagtggtagt
    14881 ggaaaatcaa agtcgatttt ccactaccat aaaatattat agactccatt ttattgaacc
    14941 aaaatttttg aagtcctccc gcttttacgt atgcctatca ctggttacta aactcgctaa
    15001 taagaatgtt tttttgtcga tctaggatgg agagaggggc aaactttttc gatctacata
    15061 cagacagtcc gatttttctg acatttccta ttcatttcta gaaggtcagc ctattacaac
    15121 aaactttttt ctgtcctcca tcatcggatt ttcctgaagg tctacatatt ggacacgtcc
    15181 gctggggctt ggtgatcgtt tgacttcttc cacgaatttt ttgattgcct catcgtcgcc
    15241 cgcagcttcg atcgtgacag agccgtcatc ttcatttttc actgttccgg tgatccctaa
    15301 ttgatccgct acgatttttg tcatatagcg aaaaccaacg ctttgtacac gtccttgcac
    15361 gttcatcttg attttgttca ttttattcac cgttctttct tttattttta aattaatgat
    15421 accgactttt ttgctactga acaagtgcaa tgatctaatc catggtataa tcagttgagg
    15481 tgaaaaatgt gaaagagatc caatcagcaa aaaatccaag aatcaaagaa ttaaaaaaat
    15541 tacataagaa aaaatatcgt gaagaaaaag gggaatatct gcttgaaggg tttcacctga
    15601 tcgaggaggc tgttgcctca aatgcgaagg tcaacgaact ttttattacg caacgcggtc
    15661 tgactgagtg ggctagttgg cttgaagaaa atcaggcgct ttctgttaca ctagtatcag
    15721 aagaagtgat ggcaactgtt tcagatctac caactccgca agggatcatg gcagtggtgg
    15781 aagctgaaaa aagagagata ccgaattttg aaggtggttg gttgttgctc gacaacgtac
    15841 aagatcccgg caatgtaggg acaatgatcc gcacagccga tgcttttggt ctatcttgtg
    15901 ttgtactagg taagggaacg gcggatgtct acagtaccaa agtattaaga agcatgcagg
    15961 ggagtaacta tcatttaccg atcatccaag aggatctaac aaaactattg cctatcctta
    16021 aagagcaagg atttataacc tatggaacag aactgaatga agaagcagtg tccctgaatg
    16081 atatccagac aacagaaaat tatgcgatcg tgatggggaa tgaaggtcaa ggcgtacgtc
    16141 cagaagtgct ggcgctaacg gataaaaatg tgtatatacc gattcaagga aatgcggagt
    16201 ctttaaatgt aggagttgca gcgggcatca tgctttatca cttctccgta tagttaagga
    16261 actattaagc tttctgcatt ttgcgaaaac ctcactagaa tattacgaac actagtggta
    16321 tactaagttc aatttcaaag ataaatggaa ttcaaaaagg tggcagatcg ataattatga
    16381 cagaaaaatg gcgtgaagac gaagaatata tgtcttatat aaatgatcta ttagaaactg
    16441 atgcagtaaa gaaattagca aattacacgc aacatgtcca ctcgacacga ttagagcatt
    16501 caatcagtgt gtcttactat agctacaaat tagcgaaaaa atggggcggt gatgcaagag
    16561 cgactgctcg agcaggcctt ttacatgatt tattttacta cgattggcgt acgacgaagt
    16621 tcgatgaagg tacacatgcg tatgttcacc caaggatcgc tgtgaaaaat gctgaaaaaa
    16681 tcacggagct ttcagatcta gagcgtgata ttattttgaa acatatgtgg ggagcaacga
    16741 ttgcccctcc taaatataaa gaaggttata tcgttacttt tgtggataaa tattgtgcag
    16801 tcaaagaagc agctcaacca atgacagctt caatgaaaca aaaatggcaa cgatatttcg
    16861 gtaaagaaca atcgatttaa ggtagttaac taagtatttg acccgagtat tttgaaaaaa
    16921 atcctcgggt tttttgttta gacattgact aattggtctt tttagtgttt actttgttga
    16981 gatgaaaaag tacataacca acataaaaat aaagggggtg atgataagat gcgatgtgta
    17041 tcttatttaa aagagtttga gggctttatc taaaggcact ttgatagaaa gatcactttt
    17101 ttcttcaaag tagccgattt tgaaagaaga aaggactcaa agtcatggaa aaaacaatca
    17161 aaccatatca atattatttt atcaagcgag aagtcgagca gctgttaagc gcctatagtt
    17221 cagtgaatga tccaaagaca gtccaaacag tccaagcatt agctgcggaa aagatccacg
    17281 aggtacttgg tcatcctaat acagctgtaa atgaatttat ggaaaaggta ctagacgtca
    17341 ctctaaaaaa agaaaaagca gaacgactat tagaggaatt gaaagtgatc gttcttccat
    17401 tcatacaacc gacaaaaccg cagatcgata aattattccg aaaagtgaaa aagttaaaac
    17461 aaccagattg gaatcaactg gatttaaggg aacatactta tattggttgg aatgatccag
    17521 gtagccaaaa aaaatttatg atctattatg aagaagaaaa actaaaaggg atcagtggaa
    17581 cattatctcc tacaatcaac aaaggaattt gttcgatttg tcaaagaact tcaaatgttt
    17641 cactctttct ttctaccaca aaaactggtt cagatggaac atacacgaaa aaaggaaatt
    17701 atatttgtca tgatagtgac caatgtaatc agcagttggt acaattagct cctttttacg
    17761 aattcacaga acgagtgaaa cgtgagcggt gatttcattg tatttttctg aaaaatatgc
    17821 tatcttattt ggtgaagtga ataggtgatg gtgttcgcct ttaaacgttg atcgtaagat
    17881 aactaatgac gcctactaaa gaaacggggc tacccctttt tttagtaggc gtttttttga
    17941 ggaggaaatg agatggaaaa tcgattacct gatccattga aggtgatcat ctatatgatc
    18001 aaatggttat tgatcacagc aattttaggt atttttatgg gaagtttatc cgcattcttt
    18061 ctaactagtt tgactgatgt gacagctatt cggctggagc atccttggtt actttttctt
    18121 ttgccgataa gtgggggact ttttgcttat ctatatacga cgtatggggg caattcttcc
    18181 cggggaaaca atttagtgat cgatcaaggg aatggaggaa atgaaaagat tccattacgg
    18241 ctgatcccac tgaccttatt tggcaccatt accacgcatc tttttggtgg ttctgttggt
    18301 cgggagggta cagcggtcca aatgggcggg gcacttgctg agaattttgc tcgttggcta
    18361 aaattgaaca aaatcgagcg tgagattctt gtcatcagtg ggatcagcgc aggttttagc
    18421 tcagtatttg ggacaccctt ggctgggaca ttgtttggat tagaagtatt ggcaatcgga
    18481 aaagtgagaa ccgaagcact gtttccaagc ttttttgcag cactttttgc gaactttgta
    18541 acggaaagtt tcaatgtcaa gcatacccat tatcaaatgg gggcgattcc tgcatgggac
    18601 ccttcacttt tcttcaaatt gttgcttgcg ggcattgcat ttggtatcat tggctggctc
    18661 tttagccgtt cgatcgtctg gttaaaagct tggtatgcaa agcggatccc ccatccagtt
    18721 ttacgcaact ttattggagg agcagtcgtg gtactcgtag tagtgatttt acagacgcaa
    18781 agatatctcg gattaagctt acctttatta caagatgcat ttaatggaag tgctcatcct
    18841 tatgattttc ttgggaaact tggctttacc gttctatcac ttggcgcggg ataccaagga
    18901 ggcgaagtga cccctttgtt tgagattggg gcaactttag gtgcgacatt agcacccatt
    18961 cttcatgtat cagtaccttt tttagcagga ttagggttta ttggggtgtt ctctggagca
    19021 acgaatacac cgattgcttg ctttgttatg gggatcgaac tttttggagg tcaagcagcc
    19081 agctacttct ttatgatctg tttgattagc tttatgtgtt caggaaatag tggaatctat
    19141 tctgcgcaaa aagttgaagt gaaaaaagga atcttgttcc taccgatcct tggaagaatc
    19201 tcacgacaaa aagagaaata aaaagaggtt gggacagaag tgtttaactc cgagaaataa
    19261 gaagaaattc acgaaaattg ctttttaaat ttttgtgaat ttcgcttatt tccgaaggag
    19321 ttgcttctgc tcccaccgtt tattcggagt tagagtctgt ggcaaaactg atttttagtt
    19381 ttgtcccacg ctctttttta ttgagcaaat ttgagaataa gagcagaaaa tactgcaaag
    19441 ccaatcatta agccacagat gataagatgg cgttttttgt gtaagacaag gatggcaata
    19501 aattctctta agatagcatt cggtaagtag tatagtgcag tttttgcacc gattccgttt
    19561 gcattgagtt tcgcttgtcg cgcatatagt ccagctctaa agatatggta attatttgaa
    19621 gtaaaaatcg cgcgataagg cccattcatt tgctgatcca ttatttcttt tgaaaacagc
    19681 atattttcta gcgtcgttgt ggaattcgtt tctactaaaa tatcacgctc gggaatccct
    19741 ttttctaaag catattgttt cattgcaatg gcttcagcta ctttttcatc ggttcctttt
    19801 cctcctgaca tgagcaagac tggcggattc aaagttgctt tactttgtgc ccaataaaat
    19861 gcgatcgcct tattgatcct tttagcaagt aggggagata cgcgttcacc atcaatcaag
    19921 ccagcgccga gaacaacgat ataatcttgg ttataccttg gacggttgaa ctgatacaaa
    19981 attgaaacag tcataaaatt aaagaaaacg atgagtagat aagcaaaaat aaacggaatg
    20041 acagcaaata gagcatttgc ccattgtggg agccaatgga ctaatgtata gtccaatatc
    20101 aaaaagatag tcaaagcgac tcctaacaag agagtcaaaa ggttggccaa agaacgggat
    20161 tcttttttca tgactacaac accattccaa aataaaaaaa tgatcaaaat gtagataccg
    20221 attgccaaaa gaattaatat tggtatcccg atcaaagcca ataacccacc gataaagata
    20281 ttttgcgtct ctattagtag ccaccctaga taaccaccaa agacaatcaa aaacgtatta
    20341 aaaagtacgc cattgattaa cctacgtttt tccttaaaat agaaaaatgc aaaaatactg
    20401 aaaaatagca atggaatcaa aaaataaaat gtatgataaa aaacttctaa aacgacgaag
    20461 taagtgacca tcatcataaa agtccacgtc ccacaataaa agaatagagc gggatcgctt
    20521 tggcgcttgc gccaatagag gatgcctgac attcctaaaa tatagataac taaaaatatc
    20581 aggtctctag taagattcat tgaatgaact cctttcgatt tgtaaatttt ttcttaaaaa
    20641 agatagcatc atttagtagt aaatatatcg aatatagaaa agttagttac taaatagtca
    20701 atcagttttt tcacgacgtt tctattataa cgaattatgt tgaaaaaagc ttttatcgaa
    20761 aacataaatc ttcaaattaa cagatttttt agacctcgat catgatgaag acttttgtag
    20821 atacaaataa ctgtaacatt gaaaatgaat gcgaaaacga tatttttttt tattggaaaa
    20881 tatcggtcaa gcaataaacc agttgcataa taaaatgtta acggcaacaa aatctgtgga
    20941 aggaatgaac cggatgattt gatggaaaaa acaagataga caagaagtat acatagtaaa
    21001 acgtggaaca agtaggccag aatgaaaaat cagtttttat ccactcgctt ttctatacat
    21061 tcactgtcac aaatataatc gaaagtaatt ctattttgag ataaagtatt ttataggaga
    21121 tagtatatga acaaaaaaag atttaaacgt attgaaagaa aatttgcatt aacttatgac
    21181 caagctaagt atttgaaaac actatttcca aaacaggtag ttcgggataa atatagtgat
    21241 cattttattt cttccttgta ttttgataca aacgatcatc actttttaaa agaaatcact
    21301 acagaaaagt cctatcgaga aaaatttaga atcagaaaat atggagatcc tcaggacata
    21361 tcgcctatct acttagagat aaaaagaaag tttcaaggaa tgacagataa acgtagaata
    21421 aaattagcgc atcatgagct cgatgatatg ttggaaaata tcgaattggc agaaaccaca
    21481 ttcagtaatt tagaagaaga gtatattttt aatgaaatac agtggttgtt cagaaggttg
    21541 gcattggagc ccaaagtata tatctattat ttaagagaag catttgtaaa tagagattca
    21601 gaagatttgc gaatcacttt tgatcaaaat atccattacc gtctaatgaa cttaacgacc
    21661 tgggaacact gtacagaaga caatctggta gcgccagaaa tcgatgtgct gatggaagta
    21721 aaaacgacag gagaaatacc tgaatggtta caggtaggat tggataggat gggtaaaaaa
    21781 gagataaaat tttctaaata taaacaagtc tataaaagat atatccaaga ggacccgctc
    21841 tatgttcaat agtgtcttaa aaaatgagcc ttctcaactc acactagatg atttcttttt
    21901 atgtatggtg gtgtcgattt tgctaggcat tctgattgct tacacccata tgtatcaaaa
    21961 caactgtaca aaaaacttca tgtttgcctt gattgggtta cctactttag tacataccgt
    22021 cattttgttg atcaacggta acttaggcac ggggatcgct gttctaggcg catttaactt
    22081 agtgcgattt cggtctgctc aagggaattc aagagatatt ttcagtattt tttgggcaac
    22141 gtcgattggt ttagccacag ggacgggata tattgtctat gcactattcg tgtctagtat
    22201 agttgctgct atccaagtca tcatcttcac gcttccaatc aaaaataaac atacagtttt
    22261 taatactgaa aaaacgatca agctaaaagt ttctaaatcc ttgtttcttt cgagtattat
    22321 tgaagaaaaa attgcagaat atacagaaaa ctgcaattta aaaaaagtaa aaatcaataa
    22381 agaaacttgt tccttacact atatagtaaa agtaaaaacg agtatgcttg aaaaagaatt
    22441 ggttcgaacg atcatggatc atccagaagt catttcgatc actgtaggta tggataaatc
    22501 gaataaatta ttgtgataac caagaaagta cttaacaatg aagtaagacc ccagttccaa
    22561 agtttagcgg ggacacttca ataaagtact ttcttttttg tcttcagcaa ctactggtaa
    22621 aactgagagc aagctttcgt gagagtacag cgataaagat caacctccct ttttatctcg
    22681 agcaatttcc ttgcaaggct gtttcgttga tcttaaacga gttagcaaca gtcaaagatg
    22741 cgatggccat aaaaaaattg cattaagttg gctggtatct gatagctggg aatctaagga
    22801 agactatcca ttctgtttct taaattccgc tagttgatag atatcagcca attgttccag
    22861 atgatcaaca tcaaagataa ccgaaagatg tttcctttta ttcccatttg cttgaagagt
    22921 ttttatcttc tctttggttc cttcactcaa tgagagtgtg actaatttct ccctcgtttt
    22981 ttctgggaaa tgaaaggata ctttgaaaaa tccgtcccaa atagaaagcc agaagatcgg
    23041 ttttactttc tgagagtggg acaaaagtaa gaaacacttt tgtctcattc tttaaaaacg
    23101 aataaacggt gggagcagaa gcaactcctt cggaaataag ccaaaattca caaaaatttg
    23161 aaaagcaatt ttcgtgaatt tcttcttatt tctcggagtt aaacacttct gtcccaacct
    23221 cttgtattca ccttttgata gccaagcttt tccatcatta taaaaccgca aatttcaaaa
    23281 aaaaacgaac aatacaaaaa gcgtattgct cgtttttctc ttcagtttct cacacctaaa
    23341 cgacttttgt ctcgacctct ttttggtgac tattcatgat gttaaaataa attactgaaa
    23401 aaatctgtga catggcgcca accaatgaca aaaatattag ctttttcaac agtagaagtg
    23461 gtaatgatgt ctgtcgaagg ctcttggttt gcttccaaat agcctaattt gtcatcagct
    23521 aattggatcg ttgctgagcc aactgtagtg cctttgttga taggagcggt cacttggtca
    23581 tccgttactt tctttttatc aattgttgga gtaatcgtta attttccagt atccattcca
    23641 ttttcgaccc agacttttac agggcttttt agcgcaacat tgacggaggt ttcttttcca
    23701 tctttcactt tgatgtcttt taggcttggg atgcttgcat ttgcttttcc aagttctttt
    23761 tctgaccagt tgtcataaca ataatccatc aatcgtgatg tttcattgaa tcgggcactt
    23821 ggattttctt catggttatc tgcatttaaa acgactgtga tgattcgtcg gccatctttg
    23881 ttgatcgttc cgacaaagca tgcacccgca aaatcagttg tgccagtttt taatccatcg
    23941 acaccttcgt gatagtttac aaaaccaggt agcatccagt tccaattgac catttcaacc
    24001 ggtgactgtg tgttttcgcc gaatgtttga tttgtggtac tcgtgatttt taagacttca
    24061 ggataatcta agatgaggtg acgagcaaca atagcgacat ctttcgcaga cagttgattt
    24121 tcatctgttt caccagtatt ctcaggtcga ttctcaccta ggtaagaatt gtttaaacct
    24181 gatgcattga cgatcgttgc atctttgata ccccaatctt ttagttgttt cttcattagc
    24241 tcaagaaatt tactttcact gcctgcaact ttttcagcta aagcaaccat ggaagcattg
    24301 gctgattgga tgaaagctga gtcaacaagt tctttcactg tataatcatt ttctttatgc
    24361 aaaggaacat tcgatagatc cggcacgaca cttaattttt ctgcataatc cgaaatggct
    24421 accttatctt cccaagataa tttaccctct ttgatttgtt caagtacgat atatagacca
    24481 atgatctttg tgatggaagc aattcccatc gttttttcgc catcctgatc ataaaggatt
    24541 tttccacttt ctgcatctac ggcaaaggca gctttggcat ttattttaaa tgcgtcgtct
    24601 gcttgaacag ttgcgctagg caagaaagaa gtgatacata agataagact tgctaatagt
    24661 acaataaaac gggtattttt tttgaacatc tttgttctcc tcatttttaa attgattctt
    24721 acaacttaga atattatcag agagaacaaa aggtttcaat ggttctgaaa aaaaccttat
    24781 aaagaacaag tcttattaat gaagaatcat agaaagttat cttttgggtt cattgacacg
    24841 aactttgact ggctgaactt cgggttcggt taggcgtgat gctgcaaaaa gggcgcctgc
    24901 tgagagtgcg accaaactaa aaatcatgaa taattgcata agatcacctc gttctttttt
    24961 gtttttttat agtatagcaa gagagtttga agctactgtt aaggaaatga gcatataatt
    25021 gatgaaagta tgaaaaaaat atgaagtagg acaacacaac taaaaatcga tcaggtattt
    25081 ctctggtagg gattcgtttt attgtattga taaaatggga tgtggtagac tttatttaca
    25141 gatacaaaga aatgattttt ttaggagggg tacgtttatg gcaaaaaaag aaaaagaatt
    25201 attatcacaa ttgacttcag aaggtaccaa aggaccgttt gtcacgatta tgttaaacac
    25261 ccatgttgcg catcaagacg tagaaaaaga tcaaatcaaa ttcaagaatt ttgcaaagga
    25321 agcgaaaaag cgctttgaaa agaaatactc tgagcatgat tggtctccat tccaagaaaa
    25381 aattactagt ttgttggacg atccgtcttt ctggcgtagt gccacaacga gtgtagctgt
    25441 catagtatca ccagaacaag tgtttatcca ccgtttgggt attcctgtag atgaccagta
    25501 ttatgtgagt gatctacctt atttattagc gatcatcaaa aatggacatt tcaactattc
    25561 atactattta ttaggattga atcgtgattc gatgaaattg tacgaagtaa cgaataaaca
    25621 agtcaaagaa gtggctttgc ctgaagatgc accaagagat gtcgtcactg ctttaggatc
    25681 tgaactaaca ggaggcgggt tgaactacgc atcaaatggt agtgaaggta cgacgttcca
    25741 tggagtcaat cctaaagatg aagaagtcga aattgattgg gtcaattatt atcaagcagt
    25801 cgatgactat ttgaaagata catttgaaaa tccagagaaa tggcctatct tcttatttgc
    25861 attaccagaa aatcagacga tgttcaaaaa agtggcgaag aacccttatt atcgttccga
    25921 tgttgcgatc tcagcttcac ctgcacaact ttcattaaaa gaaattgaag caggcgcaaa
    25981 aaaactatcg caagaattag ctgtcaaaga agcaagtgac tacaataagt tgcttgataa
    26041 aaagttcatt gatcaactcg ttgatattat accggcagca aaagaaggga aaatatccca
    26101 tttattcatt gctacatcta atttagcaga tggttttggc gaagatccag aaacagaata
    26161 tgaccgccgt caggtgctga atgaactagc agataatgtg atcacgaacg gtggtcaggt
    26221 ctttatcctt gaacaaaaag atgcaccaga cgaaaagagt ttagttgcga tcttacgtta
    26281 ttgagtgaaa actaccaaag tagatgaatg gtcaggcgtt gatagaagtg tatagctccg
    26341 agagaaggag tttcctctgt ttcagccgtt ggattcatct tgagcgagag acaaaagcta
    26401 gcttttgtct ctcgctcatt atttttgtgc tttagtccca ttggctttgc gaggggaaga
    26461 tacttttctc aaaaaaacga accttgcagt tgagaggggg atagatgcgc atactaattt
    26521 ttatcagtta aaaaatgatg tggatcatta gagaattcca aagttcgttc atcctttttc
    26581 agataataaa ttgaaatttt ttgcaaaagt gaaaatagca aatagaaatt ttctgaatct
    26641 tataggggtg atttcgagaa aacgttcgtt tatttgcaaa tatatcttct aaataagaga
    26701 ataaatacaa aaaaataagg aaaaactctg gcaattttgt aaacggtatg atatagtaag
    26761 aatgtagttt ttgttcggta atctgtggta gcttgtttca aataaacatc ttccagatat
    26821 acaccattca agtaattgca aaatatgtgt tgatacacaa ggaggatttt tattatggaa
    26881 acaggtacag taaaatggtt taactcagaa aaaggatttg gattcatcac tgcagaaaac
    26941 ggaaacgatg tattcgttca tttctcagct atccaaggcg acggattcaa aactttagaa
    27001 gaaggccaag cagtaacttt cgacgttgaa gaaggtcaac gcggacctca agcaacaaac
    27061 gttactaaag cttaatttac ctatattaaa aataaaagtc cagacattta tgtgtctggg
    27121 cttttttgtc tttttttcct taaaaagcga tgagagtatt tttctgcact ttgtagtata
    27181 atttcaaaag aacattagag gcaggagaag aggaagatat tttgaaacca agaacttatg
    27241 cagtcgttga tatcgagacg actgggacga atccaaaaga agatcggatc atccaatttg
    27301 gctgcgtcat gattgaagct ggtcggatca cttcacgttt ttcgatcgat attcatccag
    27361 ggcggaagat ttcaaaacaa atccagcatt taacagggat cacgaatcaa cgcgtccaaa
    27421 aagcgcctta ttttgaagat gtcgcccaaa cgatttacaa tcttttggca gacaccatat
    27481 ttgtcgcgca taatatttat tttgattata attttttgaa ccatgaactc atgcgatgcg
    27541 gacttcctag tttgaagata ccgggcatcg ataccgtaga acttgcacaa attttcttgc
    27601 cgacggaacc aagttttcgc ttagctgatt tgtcagaaag ccttggcttt attcatgaga
    27661 atcctcatca ggcggatagt gatgccgaag tgacaggtca gcttctttta ctgatcgaag
    27721 aacgaatgag gaaattgccg atcatcacat tagagcagat tgcacgatta agtagccata
    27781 caggaatgga tacgagtcgt ttcatttacc acgtgcttga agaaatgaaa gaaaacccgg
    27841 agccccttga tctctcatta gaaatcatcg atgggctagc tttgcagaaa aaagaagtgg
    27901 agctattcac atccgtccat tatggcgagc ggacatatcc aagaaaaaaa caagcaaaag
    27961 aaaaaatgtt tggtaaaaca ctgatgtatc gaaaagaaca gaacaggctg atgaatgctg
    28021 tctacgacca tttcacaaaa gatgaatcaa aagatttgat gatcgaagca gctacaggaa
    28081 tgggcaaaac gattggttat ctgttgcctt tgagttattt ggctacacct gaaaaaccag
    28141 ctgtcatcag caccgtttcg cttgttttgc agcagcagat catggaaaaa gacattccat
    28201 tactgaatca attgctagat cagccgatac aacctgtgat tatcaaaagc tatcgccact
    28261 atattgattt acagcgtttt aaaggtacac ttgtggaacc gccgggacaa aaacaatatg
    28321 ctttgtacca aatggctgtc ttagtttggc taacacaaac gaagaccggt gatttggatg
    28381 aattacattt aaccaatctg aaccatgcat tctttgcaga tgtcgcgcac agaggcactg
    28441 ggtttttagc aaggaatcag tcattttaca aacaagactt tgtgcgtttc ttgcaaaaga
    28501 aaatcaaaca aagcaatttt ttgatagtca accatgcatt tttagtacaa gaaacacagc
    28561 gaaaagaacc attgcttcca gctagtcctt atttgttgat tgatgaagtg catcatttgc
    28621 cggaaattgc tgaaaaagta aatgacaaac gactaagtat taactacttc cataaacaaa
    28681 ttcagcaact ggaagagccg aatcagttat ttgaccgcat acaaagttat ttgccgaagg
    28741 aacatgaagc cttgcgctgg ttgtcgattt atcgagaaga actctctgca ctatgcgaag
    28801 cgcagatttg cgtggcagag ggattaatga acttagtaag cagcaagcaa cctaacggac
    28861 aatatccaga agaagtcatg atgacaaaag aaatgttgga tcagctgcca ctggaatctg
    28921 gaacagcaat ggatcatctg caactttttt atcaagagat ccaacaacta caatcacaga
    28981 tgcgcttgac ttttgagatt atccaagaaa gttggaccaa ccagatgcgg atcgatttag
    29041 caagtttata tagcttattt gatcaattag acaagcaata cttgctgatg gaccaatggc
    29101 tagaagactg gcgagatcat ttgatccatt ggttcgttcc cgggaataca gcacagcaga
    29161 tgatttttca tctccttgat tttcaagcag ctatgttgcc gtcaactgtg tggtatcctc
    29221 gatatgacca tattctttat attggaggaa cactgaagat cggacctaat cgacagtatc
    29281 tctctaaacg acttggcatt gaagcagcac ctcttaaagt gattgcttct ccttatgatt
    29341 atgagaaaca ggcaagaatc tatgtgccga aagaagcacc tgcagtccat caagtcagca
    29401 atcaagcata tatcgattat ttagaagcag cactcacagg attgattgaa caagaagatc
    29461 ggccgatcct tgttttattc acttctcatg atgttttgca aaaggtatat cagaaaatgc
    29521 atttgtctat gctagaaaaa ggacgtgaaa ttcttgctca aggatttggc gggagtcggg
    29581 aaaaactttt gaaacgcttc attttgtcag aaaaaagcgt cttatttggt gctgacagct
    29641 tttgggaagg cgtagattta cctggagatg ctttgcagat tgttgtagtc acacgtttac
    29701 ccttcgacaa cccgttacgt ccgatggtca aagctaggaa tgcttatctg gaatcagagg
    29761 ggatcaatcc tttttatcag gaatccgtgc ctaaagcagc tttgaagtta cgccaagcat
    29821 taggtcgttt gataaggacg gagacagatc gtggcgtatt acttattttg gatcggagat
    29881 tagtcactgc aaaatatggt gcacgaatca tccatgcatt accgaaaaaa gtaccgataa
    29941 aagagctagc tatagatgaa atctatcagg atatccatga attcttaaag aaaaatgaag
    30001 aaggaagtta aaggtgccgt cttttttcat cattaaaatc tgctataatg aaaaggaatg
    30061 aagataaagg agcgacgacc cttgaagaaa aaacagacaa aggaaaaaac gattaatcgc
    30121 gtgttgcttg gcttgatcgt atttttatta gcggtcatcg tgatcagctc gattttttac
    30181 ttgcgttcca gtcgtccggc ggtccaggcg aaaaaagaag cagtagaaat cgcaaagaaa
    30241 tatgcaaatc tcgaggaagc cgatcatttt tactggttta ctcgaaagaa aacgtacttt
    30301 tcattgacgg gtacgaatga taaaggaaaa gaaatcgccg ttatcattcc taaatctggt
    30361 gaaaaagtaa aagtattgga tcaaacaaag ggactgactg aaaacgaagc aaaacagaca
    30421 attgctgcgg cccatcctga aatccaaatc gaaaaagctg ctttagggat gtatgatgat
    30481 cagcctgtgt gggaagtagt aggaaaagaa acaaatggga aactgaatta ttacctgatt
    30541 ggctttgaat ctggagatga agtcaagaca atcaagggaa tttaaaaggt aaaggaatga
    30601 atgggatgga gttatcaaaa agaacaaaac gattagaacc gtctgtcact ttggcagctg
    30661 cggcaaaggc caaggcatta aaagcaaaag gcagagatgt gctgagtttg actgtgggag
    30721 aaccggattt tgcgacacca gaaaacatcc aagaagcagc aatcgaagca atccgcaatg
    30781 ggaaagctag ctattataca ccaacagcag gaatcccaga gcttcgacag gcaatcgttg
    30841 attatctgaa gaaaaactat ggacttgtct atgaaccttc acaaacagtc gtaacagacg
    30901 gagcgaaatt tgctttgtat acccttttcc aaacgatcct tgacccgcaa gatgaagtca
    30961 tcattcctgt gccttactgg gtcagttacg gtgagcaagt aaaactggca gaaggacttc
    31021 ctgtctttgt gaaaggggaa gaatccaata gctggaaagt tactgttgag cagctagaac
    31081 aagcacgttc agaaaaaaca aaagccgtaa tcatcaattc tccatcgaat ccgactggaa
    31141 tgatttatag caagaatgaa ctgcaggcaa tcggtgaatg ggcagtaaaa aatgatattt
    31201 tgatagttgc tgatgatatt tatggccgtt tagtttataa tggcaatgag tttactccaa
    31261 ttgcaacgat ttcagaagca atcaaaaatc agaccatcat catcaatggc gtgtctaaaa
    31321 catatgcgat gacaggctgg cgcattggtt atgcggtggg gaattctgag atcatcaatg
    31381 gaatgattgc tgttgcttcc cagtcaacca gtaatccgac agcagtcagt caatatgcag
    31441 ctgttgaagc attgaaaggc gagcaagata cagtggaaga aatgcggaac gcctttgaag
    31501 aacgcctgaa tacgttatat ccgcttgtgg cagaactgcc gggtgtttct cttgaaaagc
    31561 cacaaggagc tttttatctt tttcctaatg taaaagaaac gctacgcatg tgtaaatatg
    31621 aaaatgtcac acaatgggta gaagatcttt tagaagaaac aggggtagcg cttgtgactg
    31681 gcgaaggctt tggggcacca gaaaatgtcc gcatgagtta tgccactgat cgcgctacgt
    31741 tagaagaagc ggtacgcaga atcaagcaat ttattgagag taaaagtcaa aattaatcct
    31801 gaagaaattc aaggaatata aaaggagagt catacgtgga acaaattcaa atcatcgatt
    31861 cgaaaaacca tgtgggagaa acagtaacaa tcggcgcttg ggtcgcaaat aagcgatcaa
    31921 gcggaaaaat cgcatttttg caattaagag acggaacagc ctttttccaa ggtgtagttg
    31981 taaaaagcga agtgccggaa gaagttttcc aaacagctaa aaatctacac caagaaacat
    32041 ccgtgttagt aactggagaa atccgtgaag acagtcgttc aaaatttggt tatgaaatcg
    32101 gaattacaaa tgtggaagtg gtaagcgaga gccacgatta tccgatcaca ccaaaagaac
    32161 acggaacaga cttcttgatg gatcatcgtc atttatggct gcgttcttca agacaacatg
    32221 cgattatgca gatcagaaat gaaatcattc gtgcaacgta tgaattcttt aatcaacatc
    32281 attttgtcaa aatcgatccg ccgatcttga ctggttctgc accagaaggc actacggatc
    32341 tattcgaaac aaattacttt gatcaaaaag cttatttatc tcaaagtggc caattataca
    32401 tggaagctgc tgctatggct ttcggaaaag tcttttcatt cggtccgact ttccgtgctg
    32461 aaaaatcaaa aacacgacgt catttgatcg aattctggat gatcgaacca gaaatggctt
    32521 tcactcatca agaagaaagt ttagaagtac aggaacaata tgtagcattc ttagtacaaa
    32581 atgttttaga tcattgcgaa tacgcattag atgtactagg acgagacaaa gaagtcttaa
    32641 aaacttatac aaaattgcca ttccctcgta ttacttatga tgatgcgatc gaattactga
    32701 agaaaaatgg atttgatgac atcgaatggg gcgaagactt tggttcacct catgaaacgt
    32761 tcattgcgaa ttcattcgaa cagcctgtct ttatcctaaa ctatccaaaa gccatcaagc
    32821 cattctacat gaaaccacat ccaacaagag aagacgtggt gatctgtgca gatatgatcg
    32881 caccagaagg ttacggtgaa attattggtg gaagcgaacg ggcaacagac tatgatttct
    32941 tgctagaaca aatccgtaat cacggtttgg atgaaaaaga atattcatgg tatcttgatc
    33001 tacgtaaata cggttctgtt ccgcactctg gttttggtct aggccttgaa cgaacagtca
    33061 cttggattgc tggtatcgac catgtgcgtg aagctattcc attcccacgt ttgttgaacc
    33121 gaatttatcc ataagaggtt gtgaaatcag tgccttgacc tgagtattag agaaacaaaa
    33181 cggaaaaata gcttctcata ttttactgtt ttttgagcta atatcgaggg tcagctgatt
    33241 gaacaccgtt tatatcaaga ggttgtgcgt ctgccgttca gctccgagtc acaaggagta
    33301 atttcaaaaa aacatttttt catgtttgat gactattgtg acttgtgacc aaggagttgg
    33361 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttta
    33421 aatacgggat aaaggaagtt tccagcagat aagagaagaa agcaaaagaa attgctattt
    33481 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atatacaaag aaggttattc
    33541 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
    33601 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaatga
    33661 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atggccaatc
    33721 tatcccaagc aatctagaag agatattagt aaaggaacat ttactggaag tcttagatga
    33781 gcctataaat atatgttctg atggcgtcat caagtggtct gattgacgga agcttaggtg
    33841 tgatcgatct ctctggacca aacaatgaag gaatcaaggg gaatccagca cgttaagaaa
    33901 aaaatagcat aaaaaactag acacttgatg agcgttcatt caattgtttg aagggactag
    33961 gctggtgtca ttttttatac atatattttc tctttcgtct tgatagggct tatgggataa
    34021 tataaggaag aaaatcatcg ttttattcaa aaaggagttt ttgacaaatg aaaaaacgta
    34081 tattatttat cggtgacagc attacagacg caggaagaga ccgagcagaa gcaactgatc
    34141 taggtaaagg ttatccactt ttgattgccg gaaaactaat ctcccaatat ccaacgatgg
    34201 aatttgaatg catgaaccgt gggataggag gagacaaaat tgcagatctg caagtacgct
    34261 ggcgagaaga ttgtttagct ttacaacctg actatgtatc gattttgatc ggaatcaatg
    34321 atacctggca taatactagc gatcaggcta tattcggaac tgagaaatct gctcagcaat
    34381 ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaaa agcgggaatc tgcaacatca
    34441 tcttgatgga gccgtttgta cttcctataa ccgacgaccg tcatggctgg cgtaaagact
    34501 tagaccctaa aatccaaatt gttcgtaaac ttgctaagga atatggcgca ttgctggttc
    34561 ctttagatgg catattcaat gctttaggga ttgcttatgg tttttcgaaa tatacaagag
    34621 aagatggggt ccacccaacg attggcggac atgagatcat tgcacaaagc tggatagatg
    34681 caattgccgc agtcgatttt tttagtggag ataaaaatga gcgaaagaaa agtgagaaat
    34741 gatggaaata cggtgggcag taaaagaaga tctacctcaa atcaatcagc tgattactga
    34801 tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac ctaaatgata agaataaact
    34861 attgctcgtt gctgtaacaa aagaaaaacg gattatcggt tttgcgcaag cggacgtgca
    34921 acagtcagaa gaacgggtgg gtcagcgacc atcaggaaaa ctaatccaac acacattagt
    34981 aacaaaagaa aatacggaag aaatccaaaa aaaatttgca gaatatttga tgctggaagc
    35041 taaaaaaaga agaataaaat caatttttgc ccagttttaa tcaaaaacag taatagaagg
    35101 agaaatacaa tgagttgttt aggaaatgtc atctggttta tattcggcgg actaatcggt
    35161 ggtcttagct ggttagcagc ggggatactt tggtgtatca cgatcatagg tatcccaatc
    35221 ggtatgcaat gttttaagat cgccttcctt tcttttgcgc cttttggcaa agaagtaatt
    35281 tatcaccagt ctggcatgtc attgatcgtt aatatcatct ggctgatcgt ttcaggccta
    35341 ccaattgctc tcgcacatgt cgtcagcggc atccttctat gtatcacgat catagggata
    35401 ccttttgcca agcaatcttt caaacttgct cgattggcgt tgatgccatt cggtgcagaa
    35461 attgtataaa agagaataat aaaggttaga taaagcatag agtcaggtgg gatacatgta
    35521 tttcacctga ctctatgcgt tttcctgtat aagttcttcc atgtgacttt tacgtctttt
    35581 atttatatgg ggttactgtt tatggaaagt tgaaataatt ttcattaaag aactctttct
    35641 atatttttga aagcgtagtg gagcttcgta tatctgggaa ggacattttg cagggattga
    35701 aaaaagatat tgtcctatcg aaaaattaat gaatttagac aaattgccag caacaggctt
    35761 caaacttagt tgctatcctg tcaagatcaa gaaggcaagt gcgggttgga taagagcagt
    35821 ggcaatgatc gaagagaaaa agaaagaata agccaaaaca tctgtatgga agaaattttc
    35881 cgatgcagat gtttttcatt ttttctttcg gataccttga catatgaata accattcatt
    35941 tataattagt tatgaagaga tattcatatg ttgaggcgag gaggtcatta aaatgaaaag
    36001 ttacagatta gaaggtttag attgtgcgaa ctgtgcaatg aaaatcgaaa aaggagttca
    36061 gaaaatcaat ggagtcaagg aagcaacggt aaacttcact tctggaaaac taacgattga
    36121 cgcggaggaa gatcatttgg ctacgattga acaagaaaca aaaaaagtag tgaaggaatt
    36181 agaaccagat gtcaaagtaa cagaaataga taaggaaaaa gtatctgaac atggaaatga
    36241 aaaagagaga aatacgctgt tccggatttt attttccctt gcaggtatcg ctttattgct
    36301 attatttgat tttaatgaac taattcgctt gatagggtat ttattgattt atctgctgat
    36361 aggatatgat gtagtgaaga aggcagtgat gaatatcgtc aaaggaaaaa tctttgatga
    36421 gaattttctt atgtctgttg ctacgattgg cgctatgttg atcggtgaat atccagaagc
    36481 cgtagcagtg atgctatttt atcaaatcgg cgagtatttc caaggattgg cagtcagtca
    36541 ttcaagaaaa tcgattcgcg aattgatggc gatccgtcca gaaacagctc acgtgcagac
    36601 ggcggaagga ttgatgaccg tgaaccctga agacgtgctg atcggtcagt ttgtacttgt
    36661 gaagcctggt gaacgtgtac cgttagatgg aacgatcatc gaaggagaat cattagtcga
    36721 tacgtcagca cttacaggag aatcagtccc taaatctgta tatgttggtg aaacagtatt
    36781 aagcggattt atcaataaaa ataaaccatt tttagttcaa gtagaaaaat cttatgaaaa
    36841 ttctactatc agcaaattat tagaattagt agaaaatgcc agcagtaaaa aagcaccagc
    36901 tgaaaatttc attactaaat tcgcgcgcta ttacacacca gtcgttgtag gattggccgt
    36961 tttattagct gttttacctc cacttgttgt ttctggagcg gcgttttctg aatggatcta
    37021 tcgagcattg acatttttag tgatctcatg tccatgtgca ttagtgattt ccgtgccact
    37081 tagtttcttt ggcggtatcg gaggagcaag taaaatcggt gtattagtaa aaggttcaaa
    37141 ttacttggag ttgcttgcgc aaaccgagac agtggtattc gataaaaccg gcacattaac
    37201 gaaaggcgac ttttcgatcc agacgatcga tacaaaaatc gatccaaaga tattcatgca
    37261 atatattgcg agtgcagaac agttttctac acatccgatt gctcaatctg ttttagaagg
    37321 ctacgcaggt ccgcttttac caacagctaa tatccaggaa tttgcaggtg aagggatctt
    37381 agctgaagtt gatggcaaac aagtattagt aggtaatcac aaattgatgg aaagatttga
    37441 aatctctttt ccttcaagtc aagaaatcgg tacattgctt tatctagcaa tcgatcaaag
    37501 ttacagtggt tatcttgtta ttgcagatac cttgaaagaa gatgccgtag acgcattggt
    37561 tcaattaaaa caggctggtg tgaaaaacac agtcatgttg acgggcgact cgaaaaaaat
    37621 tgctgatcat atcgggaagc aagtaggcgt ggacaagata tatagcgaat tgcttccaga
    37681 agataaagtg cagcggttag aggaaatctt acaaagtaac aataaaaaga ccgcatttgt
    37741 cggagatgga atcaacgatg caccggtgct agcaagagca gatgtaggaa tcgcaatggg
    37801 tggattggga agcgatgctg caattgaagc agcagatgta gtcatcatga atgatcaacc
    37861 atcgaagatt gcagaagcaa tccatttagc aaagaaaaca ttgaaaatcg tcaaacaaaa
    37921 cattgtcttt gctattggga taaaaatttt ggtactttcc ttaggtgctt ttggttttgc
    37981 atcaatggga gatgcagtat ttgcagatgt tggggtgaca gtattagctg ttttgaatgc
    38041 catgcgaagc ttgcatgtaa aaaataaacg ttgatcaatg aagaggacga atgaacgtcc
    38101 tctttttttt atatcctttt cttaatgaaa aatgtaaaga aaatagatag gattataaaa
    38161 taactaatgt ttttaatttt gcagaaaatt caaaatagct tgaaattcaa taaggtcaat
    38221 gaaataaaag aaaataactt atttttttga tggaaaaaat atgaaaaaac gatgtgtatt
    38281 aaataaaata atacacacat aaaataacac tgtcttgtaa gtttgttaaa aattgaatat
    38341 gattacatct ggataacaaa tttttatcat aaaggagaga acgaatgaac aacaaacaat
    38401 tacgctggtt tactgttgcg ttaattgcat ttaacatggt ttggggcatg gggaatgtag
    38461 ttaataacta tgctcagcaa ggagtatcag ttgtcacctc ttggctattg attttggcca
    38521 tttattttat tccatacgca ttgatcgttg gacaattagg atcagccttc aaggatagta
    38581 aaggaggcgt cagttcatgg gtcgaaaata cgacatctaa taaacgactg gcatactatg
    38641 ccgcttggac ttactgggtc gtccatattc cttatttggc acaaaagcct caagcaattc
    38701 tgattgctgg tggttgggca gtccaaggaa atggaaatat cgtaaatacg atgtcagtac
    38761 aaatggtagc agttatttca cttattatat ttcttgcctt tttatattta tcaacaaaag
    38821 gactttctac tttgaaagtg attggcggac tggccggaac agcgatgttc gtcatgtcga
    38881 tcttatttat tcttttagct gtaacggcac cgttcatcaa tccaacgatg gaatttgcca
    38941 caccacatat gaatcaagtg aaaacatata ttccaacatt tgatttcagt tatttcacaa
    39001 ccgtttcgat gctagtattt gcagttggag gggcggaaaa aatatcccct tatgtcaact
    39061 ctacaagaaa tcctgcaaag gaatttccta aagggatgat cttcttagca gcaatggtag
    39121 ggatttgtgc tgtattaggc tcagtcgcaa tgggtatgct gtttgctagc gataatatcc
    39181 ctaaagactt gatggcaaat ggggcataca gtgcttttca aattttaggt aattactatg
    39241 gtatcggtaa ctcattgatg atcatttacg cattgacaaa catgattgga caaatctccg
    39301 cattagcctt ttcaattgat gcaccactaa aaatcttgtt agcagatgcg gatccagaat
    39361 ttgtaccaaa ctggctacgt aaacgtaatc gtaaaggtac attggtgaat ggttatcttc
    39421 tgacaggtat actagtagga atcattattt tgttgccgat ctttggtatc aaagatatga
    39481 atgaattagt aaaatggttg acgaacttga actcagttgt catgccaatg cgttacctat
    39541 gggtgttctt ggcatatatg atgttgaata aagcttataa gaaattcaat tctgaatata
    39601 aatttgttaa aaatccaaaa atcggtttta tctttggtgc ttggtgcttt gccttcactg
    39661 cgtttgcatg tatcctagga atggtgccaa aagtagatta tactgctgat cctaaagcat
    39721 ggtggttcca attattcacg aatatcatca caccaattgt cttgatctta ttaggtatga
    39781 tcttgccagc aattgctcgt cgagacaaaa acaaagaaat tactgaacca gaaacagtgg
    39841 ttcagcctta aaatagaaat acttgttaca agaggttgtg acagaagcta cttctggaac
    39901 accgtgaatc agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca
    39961 aaaatagctc ctcatatttt gccgtttttt gagctaatgc tgaaggtcag ctgattgaac
    40021 accatttatt cgaaacaagg gactttgaca cgacttttgt cacagtccct tgtttttttg
    40081 cggcgttata agtggtatga tcgaaccaaa ttccaaagag aagtgaggga gaagaatgaa
    40141 gatcatcgtg attggacaaa gtgggtcggg aaaatcgtcc ctcgcaagga agatcaaaga
    40201 aatcaccggc tttccgcatt caggcaaaaa agtggttttt gcaagaacag caatcgttta
    40261 tagtatctaa tccgtcatgg atcgttgaag gaaattatac ttctacgtta gaggaaagga
    40321 ttaaagaagc agataagatc atttggctta aagtgccgcg ttatctcgcc atctaccgag
    40381 tagtttggcg ttctctaaaa agaaagataa acaaaaaatc tcggccagat atgcctgaat
    40441 cattttcgga gaaactaaac aggaaatact tagaattttt atcttttatc tggcattttg
    40501 aaggaaacaa tgaaccaaaa atacagcagc tgatccaagt agctaatgca agaaataaac
    40561 tgatcatcct taaaacaaga cgagagaaac aagaattttt aactaaattg aaaatagaga
    40621 gcaaaaataa ttgacagttt ttgtttacaa gcgtaatctg tattcataag ctaatgatta
    40681 catttgtaaa cgaaaggtgt gaaataaatg gaaaaagtca aaacgccagt aaagatcagt
    40741 gattcagagt gggagatcat gcgtgttatt tggacacttg gacagacgac agcccaagaa
    40801 atcacgcaga tcttagctga ttcgatggat tggaaaccag caacgatcaa aactttgctg
    40861 ggacgtttgg taaaaaaaga agtgatctgg acagaacaaa aaggaaaaaa attcatttat
    40921 catccagctg tctcggagat ggaaacagtt cgcagtgcga cggaaaatct tttttcccat
    40981 atctgtgcaa aaaggattgg agagacgatt gcggatctag tagaagaagc tactttgact
    41041 caagaagata tcaatgagat tttgaaacaa ctggaacaaa agcaaccagt agatacgatc
    41101 gaatgtaact gtatcccagg tcaatgtgaa tgtaaacaac atcaatagga ggaagaaaaa
    41161 atgaaacaac aattttcaat cgaaggtatg agctgcaatc attgtgcagc aagagtagaa
    41221 gaaacagtaa gcgcacttga cggcgtgcaa aaagtgaaag taaatttgaa aaaagcaaac
    41281 ggtacggtca agtttgatga aacaaaagtt caatccgaaa agatttgtca agcaatcaat
    41341 ggattgggtt ataaagcaga ggtgatttga tggcaacagc agaaaccaaa atggatacgt
    41401 tcgtcattac aggtatgact tgtgcaaatt gttctgctag gatcgaaaaa gagctgaacg
    41461 gacaacctgg tgtggtcaat gccacagtca acttggccac tgaaaaagca agtgtcaaat
    41521 atgaagggac gaccactgaa aaattaatcc aaagcgtaga aaacatcggt tatggtgcaa
    41581 ttttgtacga tgaagcccac aaacaaaaaa ttgcagaaga aaagcaagcg tatttgaaaa
    41641 aaatgctatt cgatcttata ttgagtacgg ttttgacttt gccgttgatg ttgtcaatga
    41701 ttgctatgat gttaggaagc catgcggcga ttgttcactt ttttcatttt ccaatcgtcc
    41761 agttagtgct gtctgctcct gtccaatttt atgtcggtgc tcgtttttat aaaggtgcgt
    41821 accatgcaat caagacgaaa gcaccaaata tggatgtatt ggtagcaatt ggaacatcgg
    41881 ctgcctttgc attgagcatt tacaacggat ttttccgtgg acacccccaa gatctttatt
    41941 ttgaaagcag cagtatgatc atcacgctga ttttgctcgg caaatatttg gaacatacag
    42001 caaaaacgaa aacaggtaat gcaatcaaac aattgatgtc tcttcaaaca aaaaccgccc
    42061 aagtgataag aaacgggaaa gaagaaacgt tagcgattga agaagtagtc gttggtgatc
    42121 aactagtcat ccgtcctggt gagcagattc cagcagatgg tcgaattatt tcaggaagca
    42181 gtgcgataga tgaaagtatg cttactggtg aaaatcttcc tgtagaaaag aatccggatg
    42241 acacgctttt tggcgggaca atcaatacga atggtctgct acacatggaa gtcacacaag
    42301 tcggcaagca gacagtatta gcgcaaatca ttcaaatggt ggaagatgct caagggagca
    42361 aagccccaat ccaaaaaatc gcagatcgga tctcagggat ttttgttccg atcgtattag
    42421 tcatcgcgtt tatcacgctc atagctacag gtttgatcac tggtgattgg caactcgcat
    42481 tgatccatag tgtctctgta ttagtcattg cgtgtccatg tgcattaggt ttagcgaccc
    42541 caacagctat tatggtagga acgggtgttg gtgcacgaaa tggaatttta attaaaggcg
    42601 gagaagcatt agaagcagct gctcacttag acagcattgt tttggataaa actggaacga
    42661 ttacagaagg aaagcccaaa gttacagatc tagtcggttc aaaagaagtc ctatctattt
    42721 tttatacttt agaacaagct tctgaacatc cactaggtaa agcaatcgtg gaatacggaa
    42781 aactgcaaga agcagcaaca tacgatatga ttgattttac agcccaccca ggtgctggga
    42841 tcagcggtac gattaatgga gtacgctatt ttgcgggtac acgtaaacga ttaattgaat
    42901 tgaacctatc atttgatgaa taccaagaac acgcgttaga attagaacag cagggaaaaa
    42961 cagtcatgtt tttagctgat gaaaagcaag tgatcgggtg gattgccgtg gctgatcaaa
    43021 tcaaactaga agccaaacaa gcaatcaagc agctccagaa taaaggatta gatgttttta
    43081 tgctgacagg agacaacaaa ctggcagccg aaacgattgg taaacaagtt ggtattgatc
    43141 caaagcatat ctttgctgaa gtacttccag aggataaagc agcctacgta gaaaaacttc
    43201 agaaagatgg caaaaaagtc ggtatggcag gcgatggaat caatgatgca ccggccttag
    43261 cattagctga tgtcggtatg gcaatgggaa gtggaacgga cattgcgatg gaaacagccg
    43321 atgtgacgct gatgaatagc agtttggcat ccattgcaca aacaatcgag ttgtctaggt
    43381 tgacattgag aaaaatcaaa caaaatcttt tctgggcttt tgtctataat acgattggta
    43441 ttccttttgc cgcactaggc tttttgaatc caatcattgc aggaggagcg atggcgttta
    43501 gttccgtcag tgtattgttg aactccctga gcttaaatcg acacatgaaa aagtaagaga
    43561 agggatggag taaaaatgaa tgggaacgat atggagaaaa aacatgagca taaaaaagaa
    43621 gtagtaaaca aagaaagtac agaatctcat gaaaaacaga tggagatgaa acatgaccat
    43681 agtcagatgg atcattccat gcacatggga cataaccatg gagatatagc aaacagcaaa
    43741 caaatggacc attcgatgca catggatcac gaacacggcg gcatggatca ttccatgcac
    43801 atggggaatt tcaaacaaaa attttggcta tcgcttatcc tagctatccc aatcattctt
    43861 ttttcaccaa tgatgggaat ggaatttcca tttcaagtaa cgtttcctgg ttctgattgg
    43921 ctcgtattga tcttagcaac tatactattt atctatggcg gtcagccatt tttaagtgga
    43981 gctaagatgg aattgaaaca aaaaagtcct gctatgatga cactgatcgc gatggggatt
    44041 acagttgcat atatttatag tgtctattcg tttatcgcaa atctgctcaa tccacataca
    44101 cacgtcatgg atttcttctg ggaattggct actttgatcg ttattatgct actaggtcac
    44161 tggattgaaa tgaatgctgt gtcgaatgcc agcaatgctt tacaaaaact agcagaatta
    44221 ctgccagaat cagtgaaacg cttgacgaaa gatggaaaag aagagacagt ttcattgaaa
    44281 gaagtaaacg aaggtgatcg tttgatcgtc cggtctggtg ataaaatgcc aacagatggt
    44341 gtaatcttga aaggtgaaac aattgttgac gaatcagctg tgactggcga atcaagagga
    44401 atcaaaaaac aagccaatga caaagtgatt ggcggttcta tcaatgggga tggaacgatc
    44461 gaaatcgaag taactggaac tggtgaaaat ggttatctag cgaaggttat ggaaatggtg
    44521 cgaaaagcac aaggagaaaa atccaagcta gagtcattat ctgacaaagt ggcaaaatgg
    44581 ctgttttatg tcgcattaat tgtgggtatc ctggcattca tcgcctggtt gttcttgaca
    44641 gatcttccta atgcattaga acgtatggta acagtcttta tcatcgcctg tcctcatgct
    44701 ctaggtttag ctatcccatt agttgttgct cgctccacat caatagcagc taaaaacgga
    44761 ttacttttga aaaaccgcaa tgcactggag caagccaatg atctagatgt gatcatgctg
    44821 gacaaaacag gaacactaac agaaggaaaa tttactgtaa cgggtgtcga agtacttgat
    44881 gatgcgttca ataaaaatga aatccttcaa tatctaggag ccttggaagc gaatgccaat
    44941 catccattgg ctgttggcat tatgaattat ttgaaagaac atgaaatcaa gccttatcaa
    45001 gctgaaaatc taaaaaatct ttcaggagtc gggctagagg caacagtcaa aaaacaacaa
    45061 gtgaaaatag ttaatgaaaa agaagttgag cgtctcggat taaacgtaga acaagcatta
    45121 ttgaaacctt accaagaaca aggaaatacc atcagcttcc tgattttaga aaatcatcta
    45181 gctgcaatcg ttgctttagg tgacgtagta aaaacagaag caaaagaatt tatccgaaca
    45241 ttgaaagaaa gaaaaatcac tcctgtcatg cttactggtg acaataaaaa cgctgcccaa
    45301 gcagtagcag attatttagg gatcgaagaa tattatggcg gtttattacc agatgataaa
    45361 gaagctgttg tccagaaata ccttgaccaa gggaaaaaag taatcatggt gggtgatggg
    45421 atcaatgatg cgccaagttt agcccgtgct tcaatcggga tggcaatcgg tgcagggaca
    45481 gatatcgcaa tcgattctgc ggatgtcgta ttgacaaaca gcgatccaaa agatattttg
    45541 cattttcttg atctagccaa gcaaacgcga aagaaaatga tccagaacct ctggtggggg
    45601 gcaggttaca atattcttgc aattccgcta gcagccggaa ttcttgcgcc aattggtatc
    45661 atattaagtc cagcagttgg tgctgtcttg atgtcgttaa gtacggtagt tgtagctcta
    45721 aatgctttga cattgaaaat caaataataa tgaaaaaagg ttgtgacaaa aaacgtgtca
    45781 caaccttttt ttcgagtaat cgatactcaa tcagctggtt ctcggcattc cagaagtaga
    45841 ttctccagaa attttatctt attttttgaa gcggctccct tttgtcagaa tttttttgta
    45901 taccgttttt cttattatac gcgttatttc taaataggtt tttttatcga attttaccgg
    45961 atctgcttgt cgtccatata tttctgcttc atattgagta gttaatcgaa tgaaacgcag
    46021 atctagcttg aattcttgtt ctattcgcag gctatattcc tgcaaagatt ctgcgggttt
    46081 gcgcggtacc aatgtttcca aagggttaag aagtttttga tatgcaatcg tattagtaga
    46141 agcaaaaaaa tgaagataga taagataact tgtccagcgc agagattttc tgaataacaa
    46201 tagaaagaac aacaagacgg ataaacacac tccaaggctg aataagacga ttttataagc
    46261 tggtgtgagt gttgtttctt ctgatggaat gacctcagat ctctcactgg aaatagtttc
    46321 tgccatattt tcgctttcat ttggttgttc gatagcaggc tggacggttt cggttgtttc
    46381 agcaactggt tgtgaaccca ttggattggc aaatgaaggg gttggttcaa atggaagcca
    46441 gccaaatcca ggaaagtata cctctggcca agaatgagca tgactattga gaatgctgta
    46501 ttcctcattc ccttgattat ctgtgcctgt tctgagccca tttgtaaaac cttttgtcca
    46561 gcgagctggg ataccaagtg ttcgaagaag gacgaccata gaagttgaaa agttgtcaca
    46621 atagccgact tttgaatcaa agaggaaata atccacataa tcgtgttctt tcggagtatg
    46681 cccggtatcc gttttggaat agcggaaatt tccgcttgtt tttaaatact gttagacagc
    46741 ttcgacttta cgaattaaag ttgcttcatt tttggtcaat tcgaccgcca agtcctcaat
    46801 acgtttaggc aaactttttg gcaattgaag atttgtctgc tgattttcta aatcatcagc
    46861 ttttaactga ctgcttttta attcttctct cgtaaaaact aacctttctg tttgtaaagt
    46921 tgcagaacgt gtcgattcat tcgggacagg aatgaatcgc tggctttcag gcaaatagta
    46981 aagcgtttgt ccactagcta atgaaaaatc agtcaaggta gtatttccat aaggataagg
    47041 taagaaagct tgttttttta gggtcaatgt aatgttttct ttgtcttcta taacggtttg
    47101 gtagctcgtg atgtcaacag ggaattcttg gattggttgg atgggtgcgt cctgatcttc
    47161 tatccatcca gaaccggtgt aatgattttt attttcgatc ttccaatagc catttccttt
    47221 ttggttcgct gtaaaaacaa cttcgccatt atcatataaa ggtccaccaa gctgttcatc
    47281 attctcactg aagcctgttc ttgcacttcc ggttaattga tactcatgga gtgtgtcata
    47341 aagcccttca tcattaagat attctcggat cggttcactc gtttcctcga gagattggtg
    47401 tactttcgga agatataaag gcaagatagc gctaaaagtt aagaaacaaa taagcagaac
    47461 tgtagtcaat gactggaatc gagtgtttcc cgacaacaac cggatggaga aaaattgcct
    47521 tgctgccaga tacacgaaca aaacagttac taccgcaatc atttcgtaca gtaaatcttt
    47581 tttccgaaaa acggtaagca tcatgaagta agccatcaaa gaaagaaagg gtgcgtacca
    47641 atagtcgtaa tccacggtca atgtaataaa taaatagatc agcaacatta tgagaaaaaa
    47701 agctgtcttt tctggcaaat aataaagttt accttgggat agatggaaga attcctctgt
    47761 aactgacgaa aaaaactgta agatccataa tatggaaaat gtcttcgaag gaaaatataa
    47821 aaacaaacaa cctatcatta ctggcaaaga aagcagcaaa cgtacccatt ttttaggaat
    47881 acagcagaca ataaggcata ggacaccaat agttaggata agaaggtagc tgttttgcaa
    47941 ttgattagct actacaaata tcggagcacc tacacttagt atcacaaagg agtaaacgcc
    48001 acgcagccat ttttcccgtt taatcgtcaa tccattcacc tcctggcaat gaacatatct
    48061 tgtctttttc ttggaccatg agttgttttt ctacgaaata aacaagataa gccgttcgat
    48121 cttttgacaa tgtttcaata gcttctgaga cttcaggaga attctcagga acaaagagga
    48181 cgatttgttt ttttgcatat tttttcaagt gtgcgaaaaa agctttctca tcgccagctt
    48241 ccaaagaagc aaaataggta tgaccggggt ctttgccatg aaaggcgcgg ttgcctaata
    48301 tcagaagttc cggctgcttt tcttttctca aatgccataa agaaaaatac aagtctagca
    48361 gaggttcaaa ttttggactg ggcgaacccc aaaagcaaaa aagaggagaa cattcttctt
    48421 cttgataatc gtattcacgg tataaccact cttgagtatg agcagacaat ttccaatcga
    48481 ttcggttgaa agaatcacct ggacgataag cgcgatgttc ccgaaaatca agacctcttt
    48541 ggtacgcgtt cagctgctgt tttttttcca gaactctctg taatttttct gcttgaatct
    48601 tacaaaaagc aggcagaaca gtcacaggaa aagagatctc tctttttgca ctttttctga
    48661 aaagtcctag actatcatag gctgtaaaaa tgacagttaa tgtttcatgg catcctcttt
    48721 cttttggctg ccaaagcaat tggacttctt gcggtttatt tgtaagaatc aaaatctctt
    48781 gtttttttct gcaaagattg ctgggaagcc gatttgtaaa cgagggagag gtaaagaaaa
    48841 agttctcttt tttgctatgg aaaaaataac ctgcatactt tttttgcgat aaacgagtgg
    48901 ctgcaatgta caagtaaccg aaagtttctt tagaaagctg cttaatgaaa tcaagctcca
    48961 tgatacataa agagtaaaaa aggccaacaa gaaccagcct aaagaattgt tgaatacaag
    49021 agcaaaaagc acaatgatga aatataccag aaataacaca aaattaatga aataatcttt
    49081 gattttttcc attgtctacc tcaatctacc tcacaggaat aggaatttgt gcaacaagag
    49141 tatgaatgat ttgtttcttt tcgtcttcct tcaatgaacg atcgtaaaaa atcaggcgat
    49201 gggaaaacac gtaaggaact aaatcctgca aatctttggg cgtgacgtaa tttctcccat
    49261 ggattaaagc ataagctttt gctgcctgaa taaaggaaag gcttcctcgt ggactgatgc
    49321 caagctggat ggcagcatga cttcttgttg ctgacactaa ctgcaaggca taagatgcca
    49381 cttcgggact gacaaagaca ttcgccacat ttgtttttat ttcttctatt tcataactgt
    49441 tcaatacttc atttaagtta tccaacactt tttcgaattt atccaaaagt agcaaaagtt
    49501 cgtcatcaaa agaaggataa ccgatttgta gtcggaataa aaaacgatcg agctgtgctt
    49561 ccggcaaagg atatgttcct tcgtactcga ttggattttg cgtagctaag acaaaaaaat
    49621 gattgtctag agggtatgta ttgttgtcga tcgttgcatg attctcggcc attgcttcta
    49681 aaagtgctga ctgagtacga ggagtcgtac gattgatctc atctgccaac aagatcgtgg
    49741 taaagatcgg tcccggacga aattcaaact cttttgattg tgaattgtat acggaaaaac
    49801 caagaatatc actaggcagc agatcaggag tacattggac tctgctgaag gttccctgta
    49861 cagcttttgc tagtgctttg accagtaacg ttttacctac accgggaata tcttcgaata
    49921 agacatgacc acctgctaat agggccacga tcatcaattg gatagttgaa cgtttgccga
    49981 tgaccgcttt ttctaattct tatatcaact gatcgatttt ttgtttggat tcttctattt
    50041 tttcttccat tttcacatca ccttctcata aaatcgct
//
LOCUS       DAZUKA010000005        45325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000005 DAZUKA010000000
VERSION     DAZUKA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.005"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..1222
                     /gene="tgt"
                     /locus_tag="ACG8PN_000217"
     CDS             77..1222
                     /gene="tgt"
                     /locus_tag="ACG8PN_000217"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4860401.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1319..1699
                     /gene="yajC"
                     /locus_tag="ACG8PN_000218"
     CDS             1319..1699
                     /gene="yajC"
                     /locus_tag="ACG8PN_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4860402.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            2004..4601
                     /gene="adhE"
                     /locus_tag="ACG8PN_000219"
                     /gene_synonym="adhC"
     CDS             2004..4601
                     /gene="adhE"
                     /locus_tag="ACG8PN_000219"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4860403.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            4830..5936
                     /locus_tag="ACG8PN_000220"
     CDS             4830..5936
                     /locus_tag="ACG8PN_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4860404.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            5953..6981
                     /locus_tag="ACG8PN_000221"
     CDS             5953..6981
                     /locus_tag="ACG8PN_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4860405.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSNEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            6987..7466
                     /locus_tag="ACG8PN_000222"
     CDS             6987..7466
                     /locus_tag="ACG8PN_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4860406.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            7482..8777
                     /locus_tag="ACG8PN_000223"
     CDS             7482..8777
                     /locus_tag="ACG8PN_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4860407.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            8788..9612
                     /locus_tag="ACG8PN_000224"
     CDS             8788..9612
                     /locus_tag="ACG8PN_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4860408.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            9777..10676
                     /locus_tag="ACG8PN_000225"
     CDS             9777..10676
                     /locus_tag="ACG8PN_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860409.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(10802..11671)
                     /locus_tag="ACG8PN_000226"
     CDS             complement(10802..11671)
                     /locus_tag="ACG8PN_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4860410.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            11824..13284
                     /gene="rhaB"
                     /locus_tag="ACG8PN_000227"
     CDS             11824..13284
                     /gene="rhaB"
                     /locus_tag="ACG8PN_000227"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4860411.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            13281..14537
                     /gene="rhaA"
                     /locus_tag="ACG8PN_000228"
     CDS             13281..14537
                     /gene="rhaA"
                     /locus_tag="ACG8PN_000228"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4860412.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            14551..15396
                     /gene="rhaD"
                     /locus_tag="ACG8PN_000229"
     CDS             14551..15396
                     /gene="rhaD"
                     /locus_tag="ACG8PN_000229"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4860413.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            15398..15709
                     /gene="rhaM"
                     /locus_tag="ACG8PN_000230"
     CDS             15398..15709
                     /gene="rhaM"
                     /locus_tag="ACG8PN_000230"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4860414.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            15791..16942
                     /gene="fucO"
                     /locus_tag="ACG8PN_000231"
     CDS             15791..16942
                     /gene="fucO"
                     /locus_tag="ACG8PN_000231"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4860415.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            17069..17827
                     /locus_tag="ACG8PN_000232"
     CDS             17069..17827
                     /locus_tag="ACG8PN_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4860416.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            17927..18571
                     /locus_tag="ACG8PN_000233"
     CDS             17927..18571
                     /locus_tag="ACG8PN_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4860417.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            18578..19585
                     /locus_tag="ACG8PN_000234"
     CDS             18578..19585
                     /locus_tag="ACG8PN_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4860418.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            19601..20443
                     /gene="kduI"
                     /locus_tag="ACG8PN_000235"
     CDS             19601..20443
                     /gene="kduI"
                     /locus_tag="ACG8PN_000235"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4860419.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            20463..21266
                     /locus_tag="ACG8PN_000236"
     CDS             20463..21266
                     /locus_tag="ACG8PN_000236"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4860420.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(21515..22579)
                     /gene="fni"
                     /locus_tag="ACG8PN_000237"
     CDS             complement(21515..22579)
                     /gene="fni"
                     /locus_tag="ACG8PN_000237"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4860421.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(22576..23661)
                     /locus_tag="ACG8PN_000238"
     CDS             complement(22576..23661)
                     /locus_tag="ACG8PN_000238"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4860422.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(23674..24651)
                     /gene="mvaD"
                     /locus_tag="ACG8PN_000239"
     CDS             complement(23674..24651)
                     /gene="mvaD"
                     /locus_tag="ACG8PN_000239"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4860423.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(24644..25588)
                     /gene="mvk"
                     /locus_tag="ACG8PN_000240"
     CDS             complement(24644..25588)
                     /gene="mvk"
                     /locus_tag="ACG8PN_000240"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4860424.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            25904..26563
                     /locus_tag="ACG8PN_000241"
     CDS             25904..26563
                     /locus_tag="ACG8PN_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4860425.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            26648..27553
                     /locus_tag="ACG8PN_000242"
     CDS             26648..27553
                     /locus_tag="ACG8PN_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4860426.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(27555..28187)
                     /locus_tag="ACG8PN_000243"
     CDS             complement(27555..28187)
                     /locus_tag="ACG8PN_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4860427.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    28338..28463
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            28507..29031
                     /gene="infC"
                     /locus_tag="ACG8PN_000244"
     CDS             28507..29031
                     /gene="infC"
                     /locus_tag="ACG8PN_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4860428.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            29103..29303
                     /gene="rpmI"
                     /locus_tag="ACG8PN_000245"
     CDS             29103..29303
                     /gene="rpmI"
                     /locus_tag="ACG8PN_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4860429.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            29356..29715
                     /gene="rplT"
                     /locus_tag="ACG8PN_000246"
     CDS             29356..29715
                     /gene="rplT"
                     /locus_tag="ACG8PN_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4860430.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            29967..31304
                     /locus_tag="ACG8PN_000247"
     CDS             29967..31304
                     /locus_tag="ACG8PN_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4860431.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            31352..33481
                     /locus_tag="ACG8PN_000248"
     CDS             31352..33481
                     /locus_tag="ACG8PN_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4860432.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            33456..34145
                     /locus_tag="ACG8PN_000249"
     CDS             33456..34145
                     /locus_tag="ACG8PN_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860433.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(34553..35230)
                     /locus_tag="ACG8PN_000250"
     CDS             complement(34553..35230)
                     /locus_tag="ACG8PN_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4860434.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(35366..35560)
                     /locus_tag="ACG8PN_000251"
     CDS             complement(35366..35560)
                     /locus_tag="ACG8PN_000251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860435.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(35610..36050)
                     /locus_tag="ACG8PN_000252"
     CDS             complement(35610..36050)
                     /locus_tag="ACG8PN_000252"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4860436.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(36054..36899)
                     /locus_tag="ACG8PN_000253"
     CDS             complement(36054..36899)
                     /locus_tag="ACG8PN_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4860437.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            37048..38466
                     /gene="pepV"
                     /locus_tag="ACG8PN_000254"
     CDS             37048..38466
                     /gene="pepV"
                     /locus_tag="ACG8PN_000254"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4860438.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(38523..39470)
                     /locus_tag="ACG8PN_000255"
     CDS             complement(38523..39470)
                     /locus_tag="ACG8PN_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4860439.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(39693..40043)
                     /locus_tag="ACG8PN_000256"
     CDS             complement(39693..40043)
                     /locus_tag="ACG8PN_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4860440.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            40243..41322
                     /gene="pepA"
                     /locus_tag="ACG8PN_000257"
     CDS             40243..41322
                     /gene="pepA"
                     /locus_tag="ACG8PN_000257"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4860441.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            41466..41786
                     /locus_tag="ACG8PN_000258"
     CDS             41466..41786
                     /locus_tag="ACG8PN_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4860442.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            41808..42272
                     /locus_tag="ACG8PN_000259"
     CDS             41808..42272
                     /locus_tag="ACG8PN_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4860443.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            42477..43082
                     /gene="ytpR"
                     /locus_tag="ACG8PN_000260"
     CDS             42477..43082
                     /gene="ytpR"
                     /locus_tag="ACG8PN_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4860444.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(43121..44410)
                     /locus_tag="ACG8PN_000261"
     CDS             complement(43121..44410)
                     /locus_tag="ACG8PN_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4860445.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            44838..45317
                     /gene="rlmH"
                     /locus_tag="ACG8PN_000262"
     CDS             44838..45317
                     /gene="rlmH"
                     /locus_tag="ACG8PN_000262"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4860446.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        14379 a         8255 c         9711 g        12980 t
ORIGIN      
        1 aacttgcaaa aagcacttga aacgattatg atggtgggag ttgtcaagat attttatata
       61 gaaagtaggg aaatgaatga cgcaacctgc cattcgctat cgtttaatca agaaagaaaa
      121 acatactggc gcgcgtttag gggaattgat cacgccacac ggaactttcc ccacaccaat
      181 gttcatgcca gtaggaacat tagctacagt aaaaacaatg tccccagaag agttgaaaga
      241 aatgggtgct ggcgttattt taagtaatac atatcattta tggctacgcc ctggtgaaga
      301 cttagtagaa gaagctggcg gactgcataa atttatgaac tgggatcaac cgattttaac
      361 agattcaggc ggctttcaag ttttttcatt aagtgatatg cggaatatcg aagaagaagg
      421 cgtgcatttc aaaaatcact tgaacggttc gcgtatgttt ttatcaccag aaaaagcaat
      481 caacatccaa aataaattgg gctctgatat catgatgagt tttgacgaat gtccaccatt
      541 cgatgaaagt tacgactacg tgaaaaaatc agtagaacgt acgtctcgat gggcagaacg
      601 aggattgaaa gctcatgcta atccagaccg tcaaggatta tttggaatca tccaaggagc
      661 agggtttgaa gatcttcgtc gtcaaagcgc gaaagatcta gtcgggatgg atttccctgg
      721 ttactcaatc ggcgggttat ctgtcgggga acctaaatca gaaatgaatc gtgtattaga
      781 attcactaca ccgttgatcc ccgaaaacaa accacgttac ttgatgggtg tcggtgctgc
      841 tgattcattg atcgatggtg ttatccgtgg agtagacatg tttgactgtg tcttgcctac
      901 tcgaatcgct cgaaatggca catgtatgac ttctcaaggc cgtctagtag tgaaaaatgc
      961 gaaatatgcc catgatttcc gtccaattga tgagaaatgt gattgttata catgtaaaaa
     1021 ctatacacgt gcctatatcc gccatttgat caaatgtgat gaaacatttg ggattcgttt
     1081 gacttcttac cacaatttat acttcttgtt gaatctaatg aaacaagtac gtcaagcaat
     1141 catggatgat aatttgcttg aatttagaga agcctttttt gaagaatacg ggttcaataa
     1201 agaaaacgca aaaagcttct aaaggaaaat cttttaaaaa ttaagtaaaa aactagtgtt
     1261 atcagcattt ttctgctaaa gtgttttttg tagatacaga attagtgagg tgaaaaaaat
     1321 gggaacatta ccaatgcttg tgatgtttat cgcgctatta gcaatgtggt tttttatgtc
     1381 acgttcgcaa aagaaacaac agcaagaacg tcaaaacttg ttagatagca tgaaaccagg
     1441 tgattcagtc gtaacgatcg gcggattaca tggagttgtt tcagaaatcg acaatgacaa
     1501 acggacagtt gttttagact gcgaagggat cttccttgag tacgatcgtg cagcaatcaa
     1561 aacggtcaaa ccagggactg tcgtaacaaa tgatgcaacg actactgttg aaacaacaga
     1621 agaaccagct gagacagttg aagtaaaaac tgaaacaaca gaaacgctgg aagagacaga
     1681 agagaaggaa gaaaaataag tatcaaagct tatttaaaga cgaggtaggg cagaaagatg
     1741 tcctacctca ttttttattc aaaaaagaaa gagataagag catgggatat attgctcaat
     1801 attgaataat cgggtgtgtc atataaataa taataaataa ctttatatat ttttctatac
     1861 aaacacctgt aaataaagca attttatatt taaaaataac tgtgaaaaaa atcacaaaaa
     1921 agtgtttaca tagaaaaata aatattgtat gatatacatg tgaattattt aacaaataac
     1981 catgacccat taggaggata atgatggcta aagtaataga aaaagaaaaa acgaagacaa
     2041 ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc aaatgcagca ttgaaagaaa
     2101 tggaaagctt tgatcaagaa aaagtagacc atatcgtgca tgaaatggca atggcagctt
     2161 tggatcaaca tatgccatta gcgaagatgg ctgtagagga aacaggccgc ggtgtttacg
     2221 aagataaagc aatcaaaaat atgtatgcgt ccgaatatat ttggcataat atcaaacatg
     2281 ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa attgctgaac
     2341 cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc gacttccaca acgattttca
     2401 aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt tgctttccat ccaagtgccc
     2461 aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct gccggagctc
     2521 ctgaaaattg tatccaatgg atcgaacatc cttcgatcga agcgacttct atgctaatga
     2581 accatccagg tatcgcgatc gtcttagcga caggtggagc tggaatggtc aagtctgctt
     2641 actctacagg aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct tatattgaaa
     2701 aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca tttgataatg
     2761 gaatgatttg tgcttctgaa caagctgtga tcgtggataa agaaatctat gcagcagtaa
     2821 aagcagaatt ccaagcacac caagtttata tcgtaaaacc agatgaattg caaaaattag
     2881 aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt ggacattctg
     2941 cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa atgctgattg
     3001 ctgaattgga aggcgtaggc ccagattatc cgctatcaag agaaaaactt tctccagttc
     3061 ttgccatgat caaagctaac aacacggacc atgcgtttga tttgtgtgaa ggtatgttga
     3121 atcttggagg attaggacat acagcagtta tccattcaga aaacgaagag ttgcatgtca
     3181 aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct gcagaaggcg
     3241 gcatcggtga tatctataac gaaatgattc cgtcattgac tttgggatgc ggttcttatg
     3301 gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc aaaactgtag
     3361 cgaaacggag aaataacatg caatggttca aattaccgcc aaaaatcttc tttgaaaaga
     3421 actctctatt gtacttagag aaaatggaaa acgttgaacg tgtcatgatc gtttgtgacc
     3481 ctggtatggt ccaattcggt tactgcgata cagttcgtga agtattggca cgacgtaaaa
     3541 atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca aataccgtat
     3601 acgcaggaac gaaactgatg gcagacttca agccagatac agtaatcgct cttggaggcg
     3661 gttctgcaat ggacgcagct aagggaatgt ggatgttcta tgaacaccca gacacatctt
     3721 tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg gacatataag atcgacaaac
     3781 cagaaaaaac acaattcgta tgtatcccga caacatcagg gactggttct gaagtaacgc
     3841 catttgcggt catcactgac agtgaaacac atgtgaaata tccgttagca gattatgcat
     3901 tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca gcatctgtca
     3961 cagcagatac cgggatggat gtgttaacac atgcaatcga atcctatgtt tcagtcatgg
     4021 cttcagatta tacacgtgga ttgagcctac aagcaatcaa gttagtcttt gactatttag
     4081 aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa aatgcacaac gcttctacaa
     4141 tggctggtat ggcgtttgct aacgcattcc taggtatttg tcactcagtc gcacataaaa
     4201 tcggcgggga atacgggatc cctcatggac ggacaaatgc gattctttta ccgcatatta
     4261 tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat gactatttcc
     4321 gtgcagatac agattacgca gatatcgcaa aattcttagg tctgaaaggc gaaacaacag
     4381 aggaattagt cgaagcttta gcaacggctg tttatgaatt aggaaaatct gtcggcatca
     4441 acatgagtct aaaagctcaa ggagtcacac aagaaacact agatacaacc gtagaccgta
     4501 tggctgaatt agcttacgaa gaccaatgta cgacagctaa tccgaaagaa ccattgatct
     4561 ctgaattgaa acagatcatc attgatgctt acaacggata agcaaaagaa agcgtagaaa
     4621 aaagataaga aaatagatga aaaagaactt ggaaaacgtt gtttctccaa gttctttttt
     4681 taatttccaa gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag
     4741 tttttgttca aaaagcgata tttatagtgg acggtattac tgctatatta aggatataaa
     4801 atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta
     4861 aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg
     4921 ataatggtga atttttgttg gactttgatg gactgaaagt agatgacaaa agctggacag
     4981 tgtggaattg gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa
     5041 aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg
     5101 ctccgcccaa aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag
     5161 acacgaaaga ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg
     5221 acatgcctcg gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga
     5281 atcagttgtg ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac
     5341 tgttgaatcg cccagaatat ttagaagaag ctcgttacca gtttatgatc catatcaaat
     5401 acttgatgga taaacgaaca ggattgtggt atcacggctg gacgtttgaa ggaaatcata
     5461 actatgccga agcattttgg gctcgtggaa actgctggtt gacgattgct attccagaga
     5521 tcattgaaat cttagaatta tcgaaagaag atgcattgcg caaattattg atcgaaacac
     5581 ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga aagcggttta tggcatacgt
     5641 tgttggatga tccttcttct tacgtagaat cttctgcaac tgcaggattt gcttacggta
     5701 ttttgaaagc ggtccataaa cgttatttac cacaagaata taaagaagtc gcttatcgtg
     5761 ctatccaagg attgcttgaa caaatcgatg aaaaaggaga agttcaaaac gtttcaatcg
     5821 ggacaggaat gggggacagt ttagattttt atcggaatat cggaatcaca gcaatgcctt
     5881 atggacagtc gttaacggtg ctttgtttga cagaattgct cgtctcttat tgttaaaaga
     5941 aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag caagttcgct tagcctaggt
     6001 ctgctaggtc taagcggctg cggaaataat tctagtgtgg caactgtcgc agatgcagat
     6061 gtaaatgaga tagttacctt gcgctttgct tatgcaagca acagccagcc agtaattgat
     6121 gcaatgaatg aatttgggcg tttagtaaaa gaaaaaacag atggttccgt acagatccag
     6181 tatttcccag atggacaact gggtggagaa cgtgaactga tcgaattgac acagtcagga
     6241 gcagtagatt tcacaaaagt aagtgcctca gcgttggaaa gtttctctaa agattattcg
     6301 attttttcag ttccttatct attcaatgat gaagaacatt tctttaaggt gatggataat
     6361 gaagaaataa tggagccaat ctaccaatcg accaaaaatt taggatttac cggattgacc
     6421 tattatgatt ctggtcagcg cagcttttat atggtagacg gtccgatcca tacgccggaa
     6481 gatttgaagg gaaagaagat ccgtgtgatg caaagcgaaa ccgctatcaa aatggtagag
     6541 cttttaggcg gttcgcccgt acctatggga agtaatgaag tgtacacgtc tcttcaatcc
     6601 aatttgatca acggatcaga aaacaatgaa tttgtcttgt atacagctgg acatggtggt
     6661 gtggctaaat actattccta tgatgagcat acacgtgtgc cagatatcat catcatgaat
     6721 gatgccatca aagaacagct aactgatgag caggaaaaag ccattgaaga agcagcgaaa
     6781 gaatcaacgg tttttgaaat tgaagctttt gcaaaagcaa ttgaagaaga aaaagcttta
     6841 gcacaagaag aacatggagt ccaattcaat gatgtagata atacaccgtt tcgagaggca
     6901 gttgccccac ttcatgatat attcaaaaac gatcctacct acaaagacct ttatcaaaag
     6961 atccaagagc aaggagcgta gctgtgatga aaatgatttt aaacaaggta ttagagatcc
     7021 tgggagctgt tttgcttgtt gggatggtcg ctgttgtttt atggcaagta ttttccagaa
     7081 cggttttagg aaatccaaat acggtcacgg aagagcttgt tcgattcggt cttgtctggt
     7141 ttgccatgtt gtcttccgcg tatgttgtgg gacaaaaagc acatttagcc gtaacggttt
     7201 taagtgaaaa attaactggc aaaaaaagta aaattttaga agttgttgtt caaagtttat
     7261 ttctggtatt tgctgcgatg atcatggtgt atggcggatg gaatgctgtc acattgacaa
     7321 tggggcagat ctcgccttct ttaagtattc ctatgggata tgtttatctg tcagtacctg
     7381 tatcgggggt actcatcatt atctatagcc tgatcaatct aatcgataca ttaagcaaca
     7441 agccagcaat tcaagaaaaa atataaaaat ggaggaataa aatggcaata gaagccggat
     7501 tagtgttatt ttttgtattt gttgtgttgc ttgtattagg gatgccgatt gctataagta
     7561 ttgcattctc atcgatgatg actttattgt tagttatacc gtttgatgtt tctgctttta
     7621 gttcagctca aaaaatggtg ggaagtatca atagtttttc gctgatcgct attccgtttt
     7681 tcgttctctc gggcattatc atgaataatg gagggatagc taagaaacta gtggattttg
     7741 cgatgttgtt cgtcgggcgc gtgccggggg ctttggctca tacgaatgtg ttagggaatg
     7801 ctttatttgg ttccatctct agctcggcca ttgccgcttc taccgcgatt ggcggagtgt
     7861 tgatccctca gcaagtggaa gaaggatatg accggaagtt tgcgacagcg gtcaatatcg
     7921 cgtcagctcc gacaggtatg gtcattcctc caagtactgc ttttatcatg tattcacttg
     7981 ttgctggtgg tgcttcgatc tctgctttgt ttatgggagg ttatctcgta ggcgctttgt
     8041 gggcgctagg aatcatgcta gtagcattca tcgttgctaa aaagaataaa taccctgttg
     8101 tcgaaaaagg acagatggct catgctggaa aaataataaa agaggcgatt cctagccttc
     8161 tattgattgt catcattatc ggtggtatct tgacggggat atttacagca gtagaagctt
     8221 cagcgatagc tgtcatttac tccctgttga ttgctatgtt ttactataaa acggtaaaac
     8281 taaaagatat acctaatatg ttgtttcaat cagtagcaat gtcagggacg atcatgttct
     8341 tattggctac ttcttccatg atgtctttcg ccatggcgtt taccggtatt cctcaggcaa
     8401 ttagttcgtt gattatgggc gtaacggata ataaatttat tattctgtta ttagtcaatc
     8461 ttgtgttatt actggtgggt atgtttatgg atgtggcgcc tgctatcttg atttttacac
     8521 caatattttt accaatcgtc acaagtgtag gggttgatcc tgtacattac gggttgtttt
     8581 caatcatgaa tctgtgtgta ggctccatca cgccgccagt aggtacagga ctatatgtcg
     8641 gtgcaagtgt aggaggcgta aaagcagagg caatgctgaa accgcttctt cctttttatg
     8701 gggttatctt agtcgtcttg tttttgatta cttacttccc gcaaatcgtc atgtggctac
     8761 ccaatatgct gagttaggag aatagttatg ttgaacatta caattagagg acatgatctt
     8821 tccgaagtcc aaacgataga agatcttgca gaaaaaacaa aagaacaagg gattcataca
     8881 ctgcagctcg ccttaggtct ttcgtttcct gaaatacctt ctggcgcaaa tgagatcaat
     8941 tctgggatgg gaaattatgt caaacgagtt ttagaaaagc aagaagtgac cgtaggaatt
     9001 ttaagttgct atatcaatat gattcatcca gatttaacga tacgacaaga gttgctgaca
     9061 aagttcgagc agtatcttcg ctatgcttct tcctttggtg cgtcgatggt tgcttcggaa
     9121 actggatgtg ttttgccgga aatccaatat acggaagaaa atttcacaga tgaagcattt
     9181 gctgaagctg tgtccgttat ccgtcgcttg gtaaaagctg gagaaaaata ccaaatgatg
     9241 gtggggatag aaccaggttt gaatcatccg gtgtattctt tagcaagggt agagcagctg
     9301 atccaagcag tagattcgga ctatctagga atcattttag atccgaccaa tttgatcaca
     9361 agtagtgatt atcaagaaca agtccaattg gtagaagaag cttttgaacg ttttggtgaa
     9421 aagatatgtg cggttcattt gaaagatttt cgtgtcgaac aggaaaaaat cgtgccggta
     9481 aatttgggag acggtgtgat cgagtacaca aaaatcaaag aaatcatcat gaagaatcgt
     9541 ccgtacctgt atgtggttct tgaagaaaca aaagatgatg ggattcgtta tggaagaagt
     9601 cttcttgaat aatgtaaaga agacgttggt taactaatat ttagaatgga gggggcgggg
     9661 ctagaagatg ccacgtctct tcttttttat aagagtatat aaaagcagct ctttttgatt
     9721 acaaaaaacg tggtataata aaattgtgca taagtgaaca aaaggaggtg cggcacatgc
     9781 tccaaaagaa aatcagcggt gaacaagtca atacgatggt ttctgatcag tatgaaatct
     9841 atcatgtcaa agatgtcgtc agccaaaata aaacgatcta tcatcatcat gatttctatg
     9901 agatccatgc tacattaaag ggagaagcga ctttttattt gaacggacga caatttacga
     9961 tcaaagcagg aagtgtgctg ctcatccatt ccaatgattt gcatcgtatc gtccgccaat
    10021 cttcagatgt attcgagcgt gtctatatct ttgtcacccc ttcttttcta gaaagtcgtt
    10081 ccacgccatg gtctaatcta tcggcttgtt ttcaaccaat gggagagaag aggagtcgtg
    10141 tattgcaaat agaacaggaa gtattgaagc aaaaattatc atttatcgac catcctcctg
    10201 ataaaaaaat ttacggtgaa gacataaaat atgaacaagc tctggtagaa tacttgattt
    10261 ttctcaatca agttgtacaa aaagaagaga gtgcgcaaga acagaatgct gtagttccta
    10321 atgagcgtgt ggaacaaatg ttacaatata tctcaaaaaa tttatctcat ccgctttctt
    10381 tagaggaaat ggaaaagcag ttttttgtca gcaaatacca tatcacacga gaatttaaaa
    10441 agtacactgg atttacgttt catcagtatg taatgaagaa aaagctgatc tatgccaagc
    10501 acttgttgcg agaataccgc agctctagtg ctgtatatag taaaagtggt ttttcttctt
    10561 atccgcattt tctgaaagca ttcaagagag agttcggcat aacgcctaaa gaatttttga
    10621 aacgtgatac acagaaacag ttggttcatt atgaacattt tgaagatgaa caatgagaat
    10681 gatcactaaa aaataaccgc agtagctgct gttgtttaca tagaagcaga aactgcggtt
    10741 attttatttt tatctatata tttcaaaata ttttgaaaaa atgacgtaca tattatatga
    10801 atcagctgaa tttacgaaag tctctcggcg atagtcctgt ttttgctttg aacaggcggc
    10861 taaagtaagc aaaattctca atccctattt cttcggaaat cattcgaacc gtaaattctg
    10921 tataccgaag aaggtcttta gcgtaagaaa gttttttgct gatcaaataa tcgattggtg
    10981 gagaaccgat aaatttggaa aaatctttgc tcagctgata tttattcaat aagaattgtt
    11041 gttctaattg atcaagtgta atcgtttctt tgaaatgttc gtctaagtat tgcttcagtt
    11101 tgtaaacgta ggcgggaata tcctcttctt cgaaatctag ctgatacttt tgcaaaatca
    11161 gttcattcag caactcgtgg atagctacag aggactggaa atcggtacgg gcattcgtgc
    11221 cttgttgcat gtggatcaat tctgtgatca aatggtggat cttgttttct gatggatctc
    11281 ctgttgaatg gaaggtgtta tggccgtctt ttgtgaactc ctgataaaat gattggacgt
    11341 tctgaccatt caaatggatc caatccattt cccatggttc ttcgctgatc gtttgataac
    11401 gctgatattc ttggcagtca ataaaaaata agtctccagc ttttagctgt tgctgattat
    11461 cattatatgt aagcgaacct ttgccagaca aagtaaactt tatcaagtaa gaaggtaaat
    11521 gtgctcgttc agtatagtaa gggattggtg ctttgaagta accgatttct tggatataaa
    11581 aaaataaatt cctgcttctt tgtgtaggcg tcaagatcac tctttttgag tcttccgacc
    11641 atgagtggtt ggctttttcc cagtaatcca tgtaaacact cccaacttgt caagataatg
    11701 caattatata ccaaaatagt gtgaagcaaa atataagatt ctgtattatg atagcattgt
    11761 caataaatga taacgcttta ttaataaagt gctaatcaac aataaagtaa aagaggtgga
    11821 gaaatgaatc caacatttgt tgccatcgat atcggtgcat caagcggacg actaatgaaa
    11881 agtacgaggg atgcaaaagg ttctttgatc ttggaagaag tacaccggtt caaaaacggc
    11941 tttcggaaca aagacggtca cgatcgctgg aagatcgatt atcttatcaa agagattttg
    12001 attggatttg aaaaattaaa gcagacaggg attgatcgcc tttttgcggg gatcgataca
    12061 tgggcagtcg attactgtct gattgatgaa caaggggatc ggataaatga tccgattgct
    12121 tatcgggaca agcgtaccaa tggagcagta gaacagtttt ctgaaatcta ttctctagat
    12181 aagttgtatg agcagacagg gattcagatc cagcctttca ataccttgtt ccaattgttt
    12241 gctgaagaaa aagaaaattt ggcaaaagca cataaattgt tactgatacc tgattattta
    12301 gggtatgtct ttactggtaa catggtgacg gagaaaacta atgcatctac tatgcagctg
    12361 ctgaatgcag ggacaaaaga atgggaaata gaacttcttg aaaaaatcgg tgtctcgaag
    12421 gaattatttc caccattgac agagccggga acgatactag gagatctgca aaaagaacaa
    12481 ttttcaaaat atgatttgcc agatgtgacc tttatcacag tagcaagtca tgatacggcg
    12541 tcagctgtat taggaacgcc aggtcaagga gaacgttggg gttatatcag cagtgggaca
    12601 tggtcgcttt tagggattga aacaaagatc gccaatgttt ctcgcaatgc tttttctgaa
    12661 aattatacga atgaatgggg tgcccataac acggtgcgtt ttttgaaaaa catcatgggg
    12721 atgtggttga tccaggaagt tgcccgaatg caagagtacg agtacagtta tgcggaattg
    12781 gcagagcttg ctgcaaaaga aaaaccgctg cagcaagtga tggaaatcaa tgaccctcga
    12841 tttttgaatc ccccaaatat gatagaagag attcagcaat actgttttga aacagagcag
    12901 ccagtccctg tctcatcggg agctatcgct cgttgtgtgt atgataatct tgctctttgt
    12961 tacgcacaag agctgatcaa agtagaacac ttaactggaa cgaaattgga aaagctgcac
    13021 atcgtagggg gcggatcgaa taatcgtcta ctgaatcaat tgacagcaga ttatgccgga
    13081 atcattgtag aagcaggacc tagtgaagcc acagcaatcg ggaatgttgc catgcaaatg
    13141 ctcagtacag gtcagttttc gaatatagaa caagcaagaa atgtaatcaa acagtcgttt
    13201 ccatgtgaaa ctttttaccc agatgaaaat aagcaaaaca cgatggaatg gtacgtgaat
    13261 aaaatcaaag gagtaatcgt atgaacacga ttgaaagaaa ttacgaacaa gcaaaagaaa
    13321 aatatgcaac tatcggtgta gatacagatg ctgtactaga aaagatacag gacatcaaaa
    13381 tttctatgca ttgctggcaa ggagatgatg tcaaaggttt cttgacaccg gatggcgaat
    13441 tgaccggtgg aatcatggcg acagggaatt ttccaggagc cgcacgaaca cctgaagagc
    13501 ttagacaaga tctggagaag gcttattcgt tgataccggg caaacacaaa ttgaaccttc
    13561 atgctatcta tttggataca gaagaatctg tcgatctaaa tgaaattgag ccgaaacatt
    13621 ttgaaaaatg ggtagagtgg gcaaaaaaag aagaaatcgg tcttgacttc aatccgactt
    13681 tcttttcgca tccaatgatg aaagacggct ttacattggc tcatccagat aaagaagttc
    13741 gtgaatactg gatcgaacac gggaaaagaa gccgcaaaat cgcggagtac tttgggaaag
    13801 aaactgggca aacaagtatc aataactttt gggtacctga tgggatgaaa gataatccga
    13861 ttgatcgttt taccccacgg gaacgtttaa tggaatcact cgatgaaatt tttacagaag
    13921 agttagatga acggtatgta caagatgcag tagaaagcaa actatttggt ttaggcgctg
    13981 aagcttatac agtcggttcc catgaatttt atatgggtta cgggttgact cgtgacaaac
    14041 ttgtttgtct agatgctgga cattttcatc caacagaagt gatttccaat aaattatctt
    14101 cattagcttt attcagcaaa ggaatcatgt tgcatgtttc ccgtccagtg cgttgggatt
    14161 cagaccatgt ggttttgatg gatgatgaat tgcaggaaat cgctaaagaa ttagtccgta
    14221 acgaattgtt ggaaaaaaca aatatcggtc tggatttctt tgatgcaacg atcaaccgta
    14281 tcgctgcttg ggtcatcggt acgagaaata cacaaaaagc actgctgaaa gctatgctgg
    14341 aaccagttga gcgattgaaa gaaatggagc tggcttttga ctttacttca agaatggctt
    14401 atacggaaga attgaaagat ttcccgtatg ccgatgtatg gaattatttc tgttacaaaa
    14461 atcaagttcc agttggtttg gattggctgg aagaagtcca ggaatatcaa aaagacgtgt
    14521 tggaattaag aaaataggag tgtggctata ttgaaaaaaa tagatatttt acaagcgcct
    14581 tatgtgaaag aaatgatcga aacgacgtcg aatctttatc gcttgggatg ggatgaaaga
    14641 aatggcggga atatttctta tttactaaaa gaagaagaga tcacaccgtt cttggatcct
    14701 gaaaaagtga tccgccgcat ctcgatgatt tttgatgcca gccgtttagc aggatgctat
    14761 tttattgtaa ctggttcagg aagatatttt aaaaatgtag cgagcgaacc tgccgagaat
    14821 cttggaatcg ttcgagttgc tgaagatggc aaaacactgg aacttttgtg ggggctggaa
    14881 aacgaatgcc ttcctacaag cgagttgcca agtcatttca tgagtcatat cgcccgttta
    14941 gaagtagacc cagataatcg gatcgttatg cattgccatg ctagtcatct attagcgatg
    15001 agttttacgc atgaattaga cgaacgttcc tttagtcgta cgctatggca aatgtgtacg
    15061 gaatgcttag ttgtttttcc agaaggaatt tccatcattc cgtggatggt tccgggaaca
    15121 aatgagattg gggaagcaac agctgaaaaa atgaaagaaa ctcgattggt tttatggcca
    15181 caacatggaa tctacgggtc aggaaaagac atggatgaag tctttggttt gatcgagaca
    15241 gcagaaaagg cagcagaagt ctatacgtac gtcaaagcgc aaggaccaat cttgcaaaca
    15301 atcacggacg aaaacctgtg gcatttagca gatgcgtttg gtgtcactcc aaaagcaggt
    15361 tatttggagg aagttcatac gaaagcaggt gtctgacatg gtgaaaaaag ctttttgcat
    15421 gaaagtttac ccagaaaaac aagaagaata caaaaaaaga catgatgaac tttggccaga
    15481 aatgcgtaca gcacttcgtg aacacggggt catcgactat tcgatctatc ttgatccgag
    15541 tacaagtttg ttatttggtt acttagtgat cgaagatgaa aagttatggg aaaaaatggc
    15601 aaatacatct atcaatcaaa aatggtggaa gtacatggaa gatatcatgg agacaaatcc
    15661 agatgcctct ccagtcagtt gggaattgat gccagtcttt gaactgtagc aacaagcaac
    15721 tgggcaaccg gttgcttggg gttctttcgt ttttattgtg aaaaataata caaattagga
    15781 ggaatagaaa atgagtaatc gaatgatttt aaatgaaaca tcgtattttg gaaaaggagc
    15841 gattgagtcg atcgtccctg aatttcagca gcgaggattc ggcaaagcag ctgtcattac
    15901 tgacaaagga ttgatcgagc acggtatagc aacaaaagta acagaacttc tagataaagc
    15961 agcaatcgct tatgctttat acgacgggat cgtacctaat ccaacgatcc aaaatgtaaa
    16021 agatggagtg gcttttgtta aagaggcaga ggcagactgt ttgatcgcaa tcggtggagg
    16081 ttcgccaatc gatacggcaa aagccattgg gattattttg acgaatccag agttttcaga
    16141 cgtcattagt ttagaaggtg ttgctgatac gaaaaatcca tgtttaccta tattagctat
    16201 accaacgact tctggaacag cagcagaagt aacgatcaac tacgtcatca cagatgaagt
    16261 aaatcaccga aaatttgttt gtgttgatcc tcatgatatc ccaatcgtcg cgttcatcga
    16321 cagcgatatg atgatgggaa tgccgaaaaa attggctgca tcaacaggta tggatgctat
    16381 gacgcatgct attgaagggt ttatcacaaa aggcgcttgg gaaatgactg atatgctgca
    16441 tttaaaagca atcgaaatca tcggccattc tttggaagct tctgtagatg gcgatcaaaa
    16501 tgggcgagaa aaaatggcat taggacagta tattgcggga atgggcttct caaatgtcgg
    16561 tttaggactg gtacacggga tggctcaccc gttaagtgca tggtacaaca ttcctcatgg
    16621 cgtagcgtgt gcagcacttt tgccaactgt catgaaatat aacaaagaat atactggaga
    16681 aaagtaccgt gagatcgctc ttgttctagg gattaaagga gcagctgaaa tgtctttaga
    16741 agatgtaaga gaagcggctt gtggtgagat cgatcggttg agcaaagcgg ttggaatccc
    16801 agaaaccatt tctgaattag gtgtaaaaga agcagatatt ccagcaattg cagaagatgc
    16861 tttgcgagat gtatgcacac caggaaatcc aagagaaacg acagtagaag aaatcattgc
    16921 tttatatcaa agtttgatgt aaaaacgaag cggatcttca acattaaatt gcctgtaaag
    16981 agaaatggta gtttatatct ggatatctct tttaaattga tggtatttcc gctataattt
    17041 ttcttataaa gaaataggag aatattatat ggaaaataaa aagccttatg gaactgtatt
    17101 gatcaaagcc tctaaaatac tagactattt agcggaaaac caagatgtct ctttgcagac
    17161 gatcgcaaag ggtgtgggaa tgacctcttc tacagtcttg aaaatactag atactttgtt
    17221 gatgatcggc tacgtaaata aaaattcaga gaaaaactat cgtttaggtg caaagcttat
    17281 tcgctacgcg aataaaaaca ttgaacaaat cgatttggta gagttgactt taccgttttt
    17341 agaacagctg cagcaaaaga tcgatgagac tatccactta ggggtattgg acaacaatga
    17401 gatcttgtac gtgaacaaac tagatccaaa acatcaaacg atccgcatgt cttcaaaaat
    17461 cggaatcacc cgaccattat acaattctgc gatgggcaaa gccgtattag cagaattcag
    17521 tgaagagcag tacactgatt atcttgaaaa gcatccgttg atcccttaca cagaatatac
    17581 gatcaccaat gcattacggt tgaaaaagga aatcaaaacg gtaaaagaaa cccacatcgc
    17641 atttgacgat gaagaaatcg aacgtgatat tttttgtata ggagcctcaa tcgtgaaaaa
    17701 cgaacaaatc attggtgcat ttagtgtaag tatgccgaaa tatcgtttaa cagatgagac
    17761 aaaagaacaa ataatcagtg ccctcaagca aacaaaaagc gaaatcgagc aaagaattca
    17821 aaaataatga aaaaagcgtt tgcaattttt cttttctgcc gtataatatg ggtatagatt
    17881 tccaagttat aaaataaaat ttctaaatag gaaataggtg gagaagatga aaaaaattga
    17941 tattttatct cgattgaaaa acgccggagt gattgcggtg gtacgcggaa aaagcaaaga
    18001 agaagctttg aatgcttgcc acgcaatcat caaaggtggt ctaactggta ttgagttgac
    18061 ttttacagtc cctcaagcag atcaagtaat caaagaactt ttgtcattct acaaagacca
    18121 accagaaata gtgattggcg caggcacagt attagacgct gtcacagctc gcttggctat
    18181 cttagctggc gcagagtata ttgttagccc ttcctttgat caagaaacgg cagagatgtg
    18241 caatctgtat caaattcctt acttaccagg atgtatgact attaccgaga tcaaaacagc
    18301 gctgaaaagc ggggtagata tcgtcaaact atttcctgga agcgcttatg gaccaagtat
    18361 catttctgcc ttcaaggcgc cgatgcctca agtcaatatc atgccaactg gcggtgtgag
    18421 tctggacaat atgaaagagt ggttcgatgc tggtgtcgta acagtcggag taggtgggaa
    18481 ccttttagca cccgcagcta caggagattt tgacaaagta acggaagtcg cgcaacagta
    18541 tgcagcaaaa atgaaagaaa tcaagaggta gctgcttatg ggaaaagtag tgacactcgg
    18601 agaaatcatg ttgcgattgt ccacagagca gggaacccgt atcgattcaa gtgaacgatt
    18661 cgctgcccat tatggcggag gggaagcaaa cgtagcaatt tctttagcta attacggaca
    18721 tgaagcgatt tttgctacaa aagtacctga taacgcattg ggaaaagcag tcaaaaagca
    18781 cttgcaatgt tatggagtag atacgaaaca ggtattatct ggtggatcaa gactaggtac
    18841 ttattatatg gaaactggta gtggcgaacg cgccgcctca gtgatctatg atcgtgccgg
    18901 atcaagtttt gctgaaatgg atcatttgga atggtcgcta gaggagttat tccaaggaat
    18961 agatatattt catgtttcag gaatcacccc agctttgtct aaaaaatggc aaaaaatgac
    19021 gaaacaggtg atagaagctg caaaaaaagc tggctgcctg atcagcttcg atatcaacta
    19081 tcgtggaaaa ttatggacac agcaagaagc cagcgaagta atccatcagc ttttaccttt
    19141 agtagatatc tgctcagctg gaaaaatgga tgctctttat ttgttaggta tcgagaaagc
    19201 gccagaagca gaagaacaac ctctaattta ttattatcaa aaaatgcagg aaaagttccc
    19261 gaatattcag gtgttttatt cgaccaagcg taccgttcat tcagcaagtg aaaatgaatt
    19321 gaaggggaca ttgtggatga accatgaata tgtggaatcc caagtccata gaatcacccc
    19381 aatcgtggat cgagtaggtg gcggagatgc ttttgcaggt ggcgtgctcc atgggatttt
    19441 atccaatatg tctccgcaag aaatcattga ttttgctacg gcagcttctg ccttgaagca
    19501 tacgatccat ggggactgca accaatttag tcaagaagaa gtcgcacaat ttttagcatg
    19561 cggctctgga aaaatcattc gatagaagga gtagaagaaa atgcaagaaa tggaaacacg
    19621 ttatacacac agtccagaag atatccgtca ttattcaacg gaacaattga gaaaagaatt
    19681 tttagtagaa aaggtatttg aaccagggaa aatcagtttg acttatacgc acaacgatcg
    19741 aatgatcttt ggtggagtga cacctactga aaaagaatta gaaatcacat tagacaaaga
    19801 actaggtgtt gattacttct tagagcgtcg tgaactaggt gtgatcaata tcggtggacc
    19861 aggattcatc gagatcgatg ggcaaaaaga agaaatgaaa aaacaagacg gatactatat
    19921 cggaaaagaa acacgacatg ttgtattttc ttctcaagat gctgaaaacc cagctaaatt
    19981 ctatattagt tctgttccag ctcatcataa atatccaaat gtaaaaatca gtatcgacca
    20041 agtaaaacca atggagactg gtgaaggcct gacactgaat gaacgtaaaa tctaccaata
    20101 catccatcca aatgtatgcg aaagctgtca attacaaatg ggttatacga ttttagaacc
    20161 aggaagcgct tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt
    20221 ctactttgat tatgccaatg aagatacacg tgtgttccac atgatgggca aaccagatga
    20281 aacaaaacat ttagttgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca
    20341 ttcaggtgta ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac
    20401 ctatacagac atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa
    20461 tgatggaatt tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg
    20521 ctgtctatgg tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg
    20581 tatttaataa cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag
    20641 gcatcgatgc aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg
    20701 ttcaacagat caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca
    20761 tcaaacggac accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg
    20821 atttaaatgc cccattcatc gtttcaaaag cagttattcc ggatatgatc gaaaaaggcg
    20881 gcggtaaaat catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg
    20941 cttatgctgc ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg
    21001 gtcaatacaa tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg
    21061 ctccattacg cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg
    21121 gacgtacgcc agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct
    21181 tggcttctag cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac
    21241 ttgcctatat cggaaaacaa ccttaaaaga gctttagtac actgtttagt ggagaggttg
    21301 tgaaatcagc ggattgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa
    21361 aaagaggctg ggacagaagc atttaactcc aagaaataag aaggaattca cgaaaattgc
    21421 ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag ttgcttttac ttccgccgtt
    21481 tgtacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttctg
    21541 tgcgtcttcc atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc
    21601 cggaaaagat cagagattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat
    21661 aaagcagacg aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt
    21721 gattcatcag atgcgtcagt acggttcctg aagtacccac ggcatttgcg ccgagacaaa
    21781 gggctttaaa tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag
    21841 tgtctgcttc atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat
    21901 aactcagttc ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc
    21961 gaccgctgat atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg
    22021 tcatgccaaa accgacttct tttacgatga gggggacgtc aatggctgtt tgagtttctt
    22081 gaataagtac tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag
    22141 gtgcattcaa atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tctttggctc
    22201 tttcgacaga agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc
    22261 gcataatcgt ataagtatcg gctaaagaag ggactttcaa ggcagcgctg actgaaccag
    22321 tggcaatcat caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg
    22381 ttttttcgct tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa
    22441 ctgcctgtgt acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga
    22501 tccgtacgaa gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat
    22561 gttcgtcttt tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag
    22621 tggagtgatt tctgcttttt cccatgcact cattaaagga agaatgccag atttctggtc
    22681 aacaattacg attccgcaat cgcccccacc tgcaccagaa gattttgcgg ctccttcata
    22741 ctgttcagct aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat
    22801 tgcagataaa tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac
    22861 attattttct ttaaaccctt tgatcatttc gttgacacat tctgtactat tttttaagaa
    22921 aagctgataa gccaccattt tatcttctct cgaacggtga acttgatcga ccaaatcaga
    22981 agtagaggca gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa
    23041 tggctcaatg gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt
    23101 ttcttgttct tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca
    23161 gctagcagcg atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc
    23221 aatcttgaaa atctccaact gagataaatt caacgcgtaa aaaacattca aggctttgac
    23281 cgttgcaacg gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc
    23341 taactcactt gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata
    23401 cttttcagtc aagcgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa
    23461 aactaattct ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg
    23521 gatacttccg acttttcgtg cagattctac agtgactgtc acgaattgat cgactgcagc
    23581 gataactgct ggatggcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc
    23641 tggtgcagat acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc
    23701 agcatgtgca gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc
    23761 agataaatgg ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta
    23821 gcatggaatg ccttgctttc tcaattgtcg caccgcatcc atcgctttta agctgtcagg
    23881 agaccagtaa gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt
    23941 tctttccatc gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg
    24001 atatagatcc ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt
    24061 ccgacgcatc ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac
    24121 cattgctaaa tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc
    24181 gtcatggcct ttttcccatt cgacgaaacc accgaaaata cttcggcaag ctgaaccaga
    24241 cccgcgtcga gcaaatcttg aaagagagag atcatctaat ccaagcttaa gagcagtatt
    24301 gcaagcgcct gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg
    24361 aacgaaattc tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag
    24421 gtcaagaaac tgactgactt tttttgttgc tttttcgtct tgcaaggtgc catctagata
    24481 aaattcatct accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc
    24541 caatgttaac gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca
    24601 atatttgatt agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta
    24661 ctcctaatcc ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt
    24721 cttgggcttt tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag
    24781 ttaatttggc acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat
    24841 cattgctgat tgtcagcttt tccagaagtg attgtgattc atccattgtc tgtgccaacg
    24901 tttctggtga attttcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt
    24961 ggatcatttg tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg
    25021 cttctcttgt ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca
    25081 aagaaaagta gtcgaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag
    25141 cggctgcatc gattccacta ggattaccat gggcgatttt ttccgaaagc tggacatttt
    25201 ctaatagtat ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg
    25261 ctgtggcgac tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg
    25321 tcagttgcaa gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga
    25381 tattttttaa tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt
    25441 gatcgtccat tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa
    25501 aggcaatcgc tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc
    25561 ttgattctcc ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt
    25621 taagcaaaaa ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact
    25681 aagggtctca aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa
    25741 gaatacgttt tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc
    25801 tttgactaga aaaaaataca attgacacta ctagatattg ggggtatatt taaaaaggtt
    25861 ggagttaaac acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt
    25921 agcaggtaca attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga
    25981 tagtcaggca ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa
    26041 ccctgaacag tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat
    26101 caaacaagca atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact
    26161 gcttttccat ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga
    26221 tgaactgctg gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga
    26281 tttattagtt catattcgtg tatcttttga tacgatgttg gagcgtatcg aaaaacgtgg
    26341 tcgttcgtat gaacaattgt cctttgatcc aagtctatac gattattaca aagaattgaa
    26401 cagacgttac gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg
    26461 agaccgctat aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga
    26521 agaaaaatta gcagagattc gccaaaccaa agtagccatt tgatttacag ttgctcaaat
    26581 aaagggtaaa cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg
    26641 atagagtatg cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca
    26701 gcaaaacgat gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat
    26761 gctgacttat tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga
    26821 agcgttactg cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac
    26881 tagtcctatc gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac
    26941 cgagtacgtg attcgactaa ttcgatcgct tttggaacga atgccagaca tgagtgtata
    27001 cagtttattg tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga
    27061 agtgaatagt gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa
    27121 tctgctgcaa ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca
    27181 aaatgtagta cttttagaac aaatcaaggc attggctatt tatcgcaagt tgtcagaaaa
    27241 ggaaaaagaa gagttggacg atgcgctgat cgaagcacga caagcagttg aaatgacgaa
    27301 cttagcttcg cagattttag accagctctc tggcacttat aataatttgc tgaataacaa
    27361 cttgaatgac acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat
    27421 tgtgacagga ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg
    27481 gggaatcgcc ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg
    27541 gcggattcgt tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct
    27601 tgctcttggt tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgctgtgaag
    27661 aaattagtcg taaagtctaa tcccttcaga gaagttcgaa accagttcga accggtcaac
    27721 tgattgtcag acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat
    27781 tgacaatgat cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca
    27841 atacaatcag tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat
    27901 ttacattgat gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa
    27961 tcacattttt caaaaatgac attactgcat tcaaaatgac gtaaaactgt tttttgcatc
    28021 gtaagttttt ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg
    28081 tcacccccgt tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc
    28141 tttgtaagaa gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc
    28201 gtttttcttt agtatagcaa agaagcggga atcactgcta gaaagtctgc taatctaatc
    28261 attttcagaa aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct
    28321 atactatgat ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg
    28381 tcactgggct agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag
    28441 agaactcgtt tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg
    28501 aggtgaatga ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta
    28561 cgattgatcg gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat
    28621 attgcagaac aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt
    28681 gcgcgaatca tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg
    28741 cgtaaaaaac aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg
    28801 aatgacttca atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg
    28861 aaagcttcta tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc
    28921 ttgaatcgct tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg
    28981 gacggacgca gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga
    29041 ctaactggct gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag
    29101 tcatgccaaa acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag
    29161 gcggattgaa acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac
    29221 aacgccgtca attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc
    29281 aacaactagc aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat
    29341 taggaggaat tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa
    29401 agagtgctaa aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca
    29461 aaagaacaag taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc
    29521 gacttccgta aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc
    29581 tactcaaaat taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta
    29641 gctgacttgg ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa
    29701 gctttggcta aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt
    29761 ctttcctctt tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt
    29821 ctaaaatagc ataattaagg ttttttaatt gaaaactgaa catttatggt caccctttaa
    29881 tgaaagcgct aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag
    29941 aaaaacaaaa tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta
    30001 tctcattctt tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttg
    30061 ccatcatggc tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaacc
    30121 cggcgatgga agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag
    30181 ctgagtacgc caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc
    30241 tagtagatag cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca
    30301 agccaagaat cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga
    30361 caggaatcgg accagtgatt tctattgcag ttattgtgtt gccaatcttg atgtcattag
    30421 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
    30481 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
    30541 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
    30601 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
    30661 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
    30721 cagttttagg ggtcgttctt ttagatcttc caatcatctt aggttttatt ctagcaggta
    30781 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
    30841 ttgcgaaatt atttacagat ggcgcagtag atgttgcgcc aatggttggt ttcttgatga
    30901 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
    30961 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
    31021 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
    31081 tattagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaatcacaa
    31141 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
    31201 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggag
    31261 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
    31321 aaaaacgatt agatgaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
    31381 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
    31441 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca
    31501 gtcttttcaa aattgtttgc tggaaaataa tatcagtcca gaagatgttg tgtttgttgc
    31561 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
    31621 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
    31681 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
    31741 acacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
    31801 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
    31861 tgaagcagca tcggtaaaat cgatccctac aacgatggct tctgacatca caaaattata
    31921 tggattgaca agaagaactc ggacagcagc aatcaatgca tctattttac caaaaatgct
    31981 agatacggca acctctacag aagattccgt acgagaagca ggggtcaatg tttctttgat
    32041 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
    32101 aaccatgctt tccggtccag cagcatcagt catgggctct ttgatgtact tgagagcgtc
    32161 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
    32221 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
    32281 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
    32341 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
    32401 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
    32461 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
    32521 caatgtcctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
    32581 aaaagccatc caagcattag ctgattattg tcatacgacg gttgaagaca tcgcagaaca
    32641 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
    32701 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
    32761 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
    32821 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
    32881 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
    32941 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacaga cttcaaaagt
    33001 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
    33061 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
    33121 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
    33181 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
    33241 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
    33301 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
    33361 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
    33421 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
    33481 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
    33541 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
    33601 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
    33661 ccgatgtagg agaaatttgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
    33721 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatt cgattgatga
    33781 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
    33841 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
    33901 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
    33961 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
    34021 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
    34081 ggatcttaga agaaataaaa aaaatagaaa aaagatgtac ggagtatgat ttagattaca
    34141 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacgatgttc
    34201 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
    34261 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaacggtgt
    34321 tcaaaagctt gcatctgcgg taataagtcc agccaagtta aaaacataag aagctatttt
    34381 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
    34441 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
    34501 tttgccgttt ctttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
    34561 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
    34621 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
    34681 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
    34741 aaatcgtcaa acgtaaccat ctgaaacgcg tactataact ttctagccaa tcccaaattt
    34801 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    34861 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
    34921 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    34981 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    35041 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    35101 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    35161 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    35221 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    35281 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    35341 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttaacac
    35401 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    35461 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    35521 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    35581 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    35641 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    35701 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    35761 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    35821 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    35881 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    35941 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    36001 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    36061 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    36121 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    36181 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    36241 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    36301 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    36361 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    36421 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    36481 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    36541 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    36601 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    36661 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    36721 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    36781 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    36841 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    36901 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    36961 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    37021 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    37081 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    37141 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    37201 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    37261 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    37321 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    37381 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    37441 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    37501 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    37561 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    37621 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    37681 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    37741 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    37801 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    37861 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    37921 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    37981 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    38041 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    38101 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    38161 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    38221 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    38281 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    38341 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    38401 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    38461 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    38521 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    38581 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    38641 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    38701 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    38761 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    38821 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    38881 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    38941 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    39001 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    39061 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    39121 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    39181 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    39241 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    39301 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    39361 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    39421 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    39481 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    39541 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    39601 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    39661 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    39721 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    39781 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    39841 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    39901 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    39961 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    40021 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    40081 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    40141 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    40201 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    40261 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    40321 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    40381 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    40441 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    40501 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    40561 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    40621 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    40681 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    40741 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    40801 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    40861 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    40921 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    40981 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    41041 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    41101 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    41161 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    41221 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    41281 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    41341 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    41401 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    41461 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    41521 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    41581 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    41641 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    41701 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    41761 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    41821 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    41881 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    41941 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    42001 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    42061 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    42121 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    42181 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    42241 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    42301 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    42361 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    42421 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    42481 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    42541 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    42601 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    42661 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    42721 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    42781 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    42841 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    42901 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    42961 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    43021 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    43081 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    43141 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    43201 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    43261 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    43321 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    43381 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    43441 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    43501 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    43561 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    43621 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    43681 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    43741 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    43801 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    43861 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    43921 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    43981 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    44041 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    44101 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    44161 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    44221 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    44281 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    44341 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    44401 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    44461 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    44521 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    44581 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    44641 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    44701 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    44761 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    44821 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    44881 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    44941 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    45001 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    45061 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    45121 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    45181 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    45241 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    45301 ctccatacca caaataatga tattt
//
LOCUS       DAZUKA010000006        41882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000006 DAZUKA010000000
VERSION     DAZUKA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.006"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1134)
                     /gene="recA"
                     /locus_tag="ACG8PN_000263"
     CDS             complement(85..1134)
                     /gene="recA"
                     /locus_tag="ACG8PN_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4860447.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1243..2484)
                     /locus_tag="ACG8PN_000264"
     CDS             complement(1243..2484)
                     /locus_tag="ACG8PN_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4860448.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2724..3302)
                     /gene="pgsA"
                     /locus_tag="ACG8PN_000265"
     CDS             complement(2724..3302)
                     /gene="pgsA"
                     /locus_tag="ACG8PN_000265"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4860449.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3511..4434)
                     /locus_tag="ACG8PN_000266"
     CDS             complement(3511..4434)
                     /locus_tag="ACG8PN_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4860450.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5173..6459)
                     /locus_tag="ACG8PN_000267"
     CDS             complement(5173..6459)
                     /locus_tag="ACG8PN_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4860451.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6452..7720)
                     /locus_tag="ACG8PN_000268"
     CDS             complement(6452..7720)
                     /locus_tag="ACG8PN_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4860452.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7972..8433
                     /gene="mscL"
                     /locus_tag="ACG8PN_000269"
     CDS             7972..8433
                     /gene="mscL"
                     /locus_tag="ACG8PN_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4860453.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8492..8938)
                     /locus_tag="ACG8PN_000270"
     CDS             complement(8492..8938)
                     /locus_tag="ACG8PN_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4860454.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9031..9276)
                     /locus_tag="ACG8PN_000271"
     CDS             complement(9031..9276)
                     /locus_tag="ACG8PN_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4860455.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9391..9969)
                     /locus_tag="ACG8PN_000272"
     CDS             complement(9391..9969)
                     /locus_tag="ACG8PN_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4860456.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10046..10576)
                     /locus_tag="ACG8PN_000273"
     CDS             complement(10046..10576)
                     /locus_tag="ACG8PN_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4860457.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10580..11440)
                     /gene="rlmB"
                     /locus_tag="ACG8PN_000274"
     CDS             complement(10580..11440)
                     /gene="rlmB"
                     /locus_tag="ACG8PN_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4860458.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11433..11828)
                     /locus_tag="ACG8PN_000275"
     CDS             complement(11433..11828)
                     /locus_tag="ACG8PN_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4860459.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11825..13237)
                     /gene="cysS"
                     /locus_tag="ACG8PN_000276"
     CDS             complement(11825..13237)
                     /gene="cysS"
                     /locus_tag="ACG8PN_000276"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4860460.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13234..13770)
                     /gene="epsC"
                     /locus_tag="ACG8PN_000277"
     CDS             complement(13234..13770)
                     /gene="epsC"
                     /locus_tag="ACG8PN_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4860461.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13856..14095)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14092..15552)
                     /gene="gltX"
                     /locus_tag="ACG8PN_000278"
     CDS             complement(14092..15552)
                     /gene="gltX"
                     /locus_tag="ACG8PN_000278"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4860462.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15722..16879)
                     /locus_tag="ACG8PN_000279"
     CDS             complement(15722..16879)
                     /locus_tag="ACG8PN_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4860463.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16913..18283)
                     /gene="radA"
                     /locus_tag="ACG8PN_000280"
     CDS             complement(16913..18283)
                     /gene="radA"
                     /locus_tag="ACG8PN_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4860464.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18346..18873)
                     /locus_tag="ACG8PN_000281"
     CDS             complement(18346..18873)
                     /locus_tag="ACG8PN_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4860465.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19103..19909
                     /gene="proB"
                     /locus_tag="ACG8PN_000282"
     CDS             19103..19909
                     /gene="proB"
                     /locus_tag="ACG8PN_000282"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4860466.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19906..21153
                     /locus_tag="ACG8PN_000283"
     CDS             19906..21153
                     /locus_tag="ACG8PN_000283"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4860467.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21229..21573
                     /locus_tag="ACG8PN_000284"
     CDS             21229..21573
                     /locus_tag="ACG8PN_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860468.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21690..22016)
                     /locus_tag="ACG8PN_000285"
     CDS             complement(21690..22016)
                     /locus_tag="ACG8PN_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860469.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22631..23833)
                     /locus_tag="ACG8PN_000286"
     CDS             complement(22631..23833)
                     /locus_tag="ACG8PN_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4860470.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23950..24465
                     /locus_tag="ACG8PN_000287"
     CDS             23950..24465
                     /locus_tag="ACG8PN_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4860471.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24664..25110)
                     /locus_tag="ACG8PN_000288"
     CDS             complement(24664..25110)
                     /locus_tag="ACG8PN_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4860472.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25157..26635)
                     /gene="msr(C)"
                     /locus_tag="ACG8PN_000289"
     CDS             complement(25157..26635)
                     /gene="msr(C)"
                     /locus_tag="ACG8PN_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4860473.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(26989..27618)
                     /locus_tag="ACG8PN_000290"
     CDS             complement(26989..27618)
                     /locus_tag="ACG8PN_000290"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4860474.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27602..28066)
                     /locus_tag="ACG8PN_000291"
     CDS             complement(27602..28066)
                     /locus_tag="ACG8PN_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4860475.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28553..29821)
                     /locus_tag="ACG8PN_000292"
     CDS             complement(28553..29821)
                     /locus_tag="ACG8PN_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4860476.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30052..30789)
                     /locus_tag="ACG8PN_000293"
     CDS             complement(30052..30789)
                     /locus_tag="ACG8PN_000293"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4860477.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30856..31425)
                     /locus_tag="ACG8PN_000294"
     CDS             complement(30856..31425)
                     /locus_tag="ACG8PN_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4860478.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31553..32425
                     /locus_tag="ACG8PN_000295"
     CDS             31553..32425
                     /locus_tag="ACG8PN_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4860479.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32767..33639
                     /locus_tag="ACG8PN_000296"
     CDS             32767..33639
                     /locus_tag="ACG8PN_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4860480.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33636..35594
                     /gene="nagE"
                     /locus_tag="ACG8PN_000297"
     CDS             33636..35594
                     /gene="nagE"
                     /locus_tag="ACG8PN_000297"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4860481.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35926..36777)
                     /locus_tag="ACG8PN_000298"
     CDS             complement(35926..36777)
                     /locus_tag="ACG8PN_000298"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4860482.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36900..37367
                     /locus_tag="ACG8PN_000299"
     CDS             36900..37367
                     /locus_tag="ACG8PN_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4860483.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37630..38112)
                     /locus_tag="ACG8PN_000300"
     CDS             complement(37630..38112)
                     /locus_tag="ACG8PN_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4860484.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(38212..39075)
                     /locus_tag="ACG8PN_000301"
     CDS             complement(38212..39075)
                     /locus_tag="ACG8PN_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4860485.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39673..40548
                     /locus_tag="ACG8PN_000302"
     CDS             39673..40548
                     /locus_tag="ACG8PN_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4860486.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(40693..40845)
                     /locus_tag="ACG8PN_000303"
                     /pseudo
     CDS             complement(40693..40845)
                     /locus_tag="ACG8PN_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(41113..>41882)
                     /locus_tag="ACG8PN_000304"
     CDS             complement(41113..>41882)
                     /locus_tag="ACG8PN_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4860487.1"
                     /translation="AVLKSFNVLIPIIITITSFALLSFIIIQLFNMDINKLITTIVTE
                     PLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQNMQENMAAYANKEQI
                     PHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLSVTPAIFNISEPIIF
                     GLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWTTPPVISGFLATAGD
                     WRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        12052 a         8821 c         7493 g        13516 t
ORIGIN      
        1 cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc
       61 agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt
      121 ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga
      181 aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg
      241 accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc
      301 tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac
      361 ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt
      421 accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt
      481 tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc
      541 tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc
      601 agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc
      661 catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat
      721 atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc
      781 aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc
      841 taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat
      901 agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg
      961 tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat
     1021 tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct
     1081 cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat
     1141 aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat atctttttta
     1201 gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg
     1261 ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct
     1321 ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt
     1381 tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta
     1441 atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac
     1501 tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag
     1561 cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc
     1621 ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt
     1681 cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc
     1741 ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac
     1801 atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat
     1861 cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc
     1921 aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc
     1981 tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag
     2041 aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt
     2101 tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt
     2161 tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag
     2221 agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa
     2281 tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa
     2341 acgctgctga ttatctccta caactgtttg ataataaact tcgataccta gatcagctag
     2401 ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc
     2461 gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt
     2521 ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc
     2581 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
     2641 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
     2701 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
     2761 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
     2821 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
     2881 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
     2941 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
     3001 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
     3061 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
     3121 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
     3181 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca
     3241 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
     3301 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
     3361 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
     3421 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
     3481 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
     3541 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
     3601 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
     3661 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
     3721 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
     3781 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
     3841 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
     3901 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
     3961 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
     4021 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
     4081 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
     4141 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
     4201 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
     4261 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
     4321 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
     4381 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
     4441 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
     4501 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
     4561 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
     4621 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
     4681 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
     4741 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
     4801 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
     4861 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
     4921 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     4981 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
     5041 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
     5101 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
     5161 taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa
     5221 tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc
     5281 aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga
     5341 atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc
     5401 tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt
     5461 ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa
     5521 ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga
     5581 agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc
     5641 tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt
     5701 aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg
     5761 tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat
     5821 caatttctca ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa
     5881 cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc
     5941 cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat
     6001 ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt
     6061 gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt
     6121 cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc
     6181 attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa
     6241 caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc
     6301 atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt
     6361 gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata
     6421 aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa
     6481 agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc
     6541 tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca
     6601 aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca
     6661 gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta
     6721 attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga
     6781 cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt
     6841 tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga
     6901 ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca
     6961 gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt
     7021 gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg
     7081 cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg
     7141 ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga
     7201 ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg
     7261 cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt
     7321 tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata
     7381 aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac
     7441 cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata
     7501 atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat
     7561 aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt
     7621 gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta
     7681 agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac
     7741 agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc
     7801 ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt
     7861 atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta
     7921 ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa
     7981 gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt
     8041 ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata
     8101 ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt
     8161 gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt
     8221 acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag
     8281 aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat
     8341 cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta
     8401 gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga
     8461 ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt
     8521 cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact
     8581 cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt
     8641 tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt
     8701 cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt
     8761 aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt
     8821 catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga
     8881 cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta
     8941 agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg
     9001 aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc
     9061 aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc
     9121 aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc
     9181 tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc
     9241 taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta
     9301 taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat
     9361 aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt
     9421 ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa
     9481 acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc
     9541 tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat
     9601 tgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt
     9661 ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa
     9721 cagagaatag atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt
     9781 gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc
     9841 ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata
     9901 tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc
     9961 ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat
    10021 tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt
    10081 gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat
    10141 gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt
    10201 cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc
    10261 ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat
    10321 aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat
    10381 tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat
    10441 atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct
    10501 gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca
    10561 acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca
    10621 aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca
    10681 attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta
    10741 aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa
    10801 atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg
    10861 caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa
    10921 ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa
    10981 tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt
    11041 ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg
    11101 caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa
    11161 cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc
    11221 ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg
    11281 cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg
    11341 actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc
    11401 cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga
    11461 atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc
    11521 atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc
    11581 gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga
    11641 tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt
    11701 gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca
    11761 tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa
    11821 tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt
    11881 tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt
    11941 gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata
    12001 aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg
    12061 cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg
    12121 tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat
    12181 tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc
    12241 ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca
    12301 tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca
    12361 agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt
    12421 tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag
    12481 cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt
    12541 ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa
    12601 cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct
    12661 cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt
    12721 cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt
    12781 tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat
    12841 agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc
    12901 ttggatgcag tgtagcaggt tcgacattca acgctttcgt atcttcttca aatgctcgga
    12961 tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt
    13021 tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat
    13081 agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat
    13141 acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt
    13201 ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc
    13261 tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt
    13321 caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat
    13381 ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc
    13441 ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc
    13501 ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc
    13561 tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa
    13621 taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa
    13681 accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc
    13741 tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat
    13801 gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa
    13861 agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac
    13921 gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg
    13981 tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc
    14041 cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag
    14101 tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa
    14161 ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc
    14221 ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac
    14281 tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc
    14341 agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga
    14401 cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt
    14461 cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata
    14521 aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac
    14581 ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa
    14641 cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa
    14701 taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat
    14761 aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat
    14821 caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg
    14881 ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg
    14941 atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa
    15001 gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc
    15061 gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt
    15121 ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc
    15181 tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta
    15241 tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga
    15301 ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa
    15361 ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt
    15421 atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa
    15481 tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg
    15541 tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc
    15601 gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt
    15661 tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt
    15721 tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg
    15781 catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa
    15841 cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt
    15901 catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca
    15961 tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa
    16021 caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac
    16081 catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt
    16141 ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt aggatatcta
    16201 atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt
    16261 cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat
    16321 cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt
    16381 ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt
    16441 ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca
    16501 atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa
    16561 tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac
    16621 cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt
    16681 tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc
    16741 ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct
    16801 gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag
    16861 taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga
    16921 acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg
    16981 cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga
    17041 cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt
    17101 caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga
    17161 cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt
    17221 aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag
    17281 atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg
    17341 tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag
    17401 cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt
    17461 cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt
    17521 tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg
    17581 tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac
    17641 cgacgataaa aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt
    17701 ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga
    17761 tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca
    17821 tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta
    17881 attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg
    17941 ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa
    18001 tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt
    18061 ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt
    18121 tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga
    18181 ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc
    18241 cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta
    18301 tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga
    18361 accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa
    18421 aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt
    18481 ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat
    18541 cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc
    18601 aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg
    18661 tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa
    18721 cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa
    18781 atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata
    18841 tttagaaata acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc
    18901 ctattctaac atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg
    18961 ctatcttaaa gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa
    19021 aactattttt tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt
    19081 ggatatttga ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt
    19141 caaagtagga acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga
    19201 tcagttagct tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc
    19261 atcaggagct gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat
    19321 tcctgaacag caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca
    19381 aagattcagt gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga
    19441 ttatcctgaa agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac
    19501 gatccctatc gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa
    19561 atttggcgat aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt
    19621 gatcgtcctt tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc
    19681 tcagatgtat cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc
    19741 tggtagcaca tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt
    19801 tgaagcaaac agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat
    19861 tcttgcagga aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg
    19921 caattaggac aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc
    19981 aaaaatgagc tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt
    20041 gaagcaaatc ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa
    20101 gaccgcttgc agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt
    20161 gcttcgttag acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg
    20221 agaatcggca aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca
    20281 aatgtcacga cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc
    20341 ggtggcaaag aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg
    20401 gtcagtgctg gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact
    20461 gcaggacagt tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca
    20521 aaattaatcc aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga
    20581 aataatcacg tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc
    20641 aatgcaaaag cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct
    20701 gagatcgcac cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct
    20761 ttacgtgcgg atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca
    20821 gattgggata ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat
    20881 gaagcaattg cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat
    20941 agttatgcgg ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat
    21001 gcctccactc gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct
    21061 actcaaaagt tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat
    21121 atcatttatg gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc
    21181 ttttgccttt tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt
    21241 ttaatgattt tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt
    21301 attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg
    21361 tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac
    21421 atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc
    21481 gcttatacga ttacgctact gattgctggg atttttcact tatcttataa atatcagctg
    21541 aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc
    21601 cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca
    21661 gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct
    21721 tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc
    21781 atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc
    21841 cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc
    21901 gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat
    21961 tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat
    22021 tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt
    22081 cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa
    22141 taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga
    22201 aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt
    22261 ggtaaacggc gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag
    22321 aagctatttt tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc
    22381 tgttaaacgg tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga
    22441 ggaactattt ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc
    22501 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    22561 tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
    22621 ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac
    22681 atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt
    22741 caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat
    22801 agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca
    22861 gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg
    22921 gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta
    22981 aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa
    23041 tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa
    23101 tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga
    23161 ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta
    23221 cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa
    23281 acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac
    23341 cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt
    23401 tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag
    23461 cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc
    23521 cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac
    23581 cgatccgact agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac
    23641 tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta
    23701 agattgggtc tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta
    23761 gcgctgaact tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct
    23821 ccaatatttc catccctttc tctctattag catcatacat gatttatttt acatgtcaat
    23881 atgcacatgt aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt
    23941 aggattttta tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt
    24001 tctgggtatg aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc
    24061 tatacaacgc ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag
    24121 cactctcaaa aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg
    24181 atcaaagagg aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct
    24241 ttttcgacgc tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg
    24301 aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa
    24361 gaatttggac gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa
    24421 tggtgtcagc aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc
    24481 tttctagaaa tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa
    24541 aagaaaaagc gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga
    24601 actgcattca agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt
    24661 ccatcattca ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat
    24721 caactgcaac ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa
    24781 taattgatca ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat
    24841 tcctaaaacc atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac
    24901 tggtagttta aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa
    24961 agccaccacg aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg
    25021 cagccataaa ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa
    25081 aattattcct tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa
    25141 aatcatcgta gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta
    25201 tttcataaac ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa
    25261 tcgttcccgg atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa
    25321 aattagtcgg ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg
    25381 tcagacgggt cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag
    25441 caaaccctaa gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca
    25501 tgacaaaacg aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt
    25561 gaaagcttac tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata
    25621 aggttgtctt acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact
    25681 gaaaatcacc atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca
    25741 tgatgggata gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca
    25801 tctgctgcgg cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat
    25861 tcttttggat ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccagtc
    25921 tgatttgttg ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct
    25981 tttcttggat cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta
    26041 gctgagtctt tttctccaat tccttttgag tgagataggc actataattc cccgaaaact
    26101 ctcggatttc tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc
    26161 gatcatgact aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat
    26221 caatactttt tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag
    26281 aagaaagaag acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg
    26341 aagtttcttc cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt
    26401 cagcgagata ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat
    26461 gatcgggaaa aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt
    26521 ctcctatgat ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta
    26581 gctggttgcc aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt
    26641 ttacactcct atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct
    26701 gtttgcaaaa aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat
    26761 caccaaatct gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc
    26821 atccatgaaa ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt
    26881 cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac
    26941 acacgattca aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacat
    27001 agtgatcttt tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat
    27061 tgataaaata ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg
    27121 atctgcttcg aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat
    27181 gaattgcata gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga
    27241 aaaactcttg taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat
    27301 cttccctgat tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc
    27361 gccaaaaatc atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa
    27421 aggacgcaag atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggtttcat
    27481 attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg
    27541 gattttgtat gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa
    27601 atcatttttt tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt
    27661 gggtattcaa tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg
    27721 gggatagcac ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg
    27781 ctttttctgt tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa
    27841 catagccatt tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca
    27901 acttttcttg caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct
    27961 cgtatctagt aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg
    28021 ctaatttgat ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc
    28081 taataattga ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca
    28141 aagaatcagc tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt
    28201 ttttgtagaa tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg
    28261 attcttatca aactatagaa gatagctctt ctgcagtaga taattttaca ccgccaaaaa
    28321 tttccgaaat cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt
    28381 aaacagtaat aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt
    28441 tcaaaaactt gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt
    28501 tgtttggctg ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac
    28561 cataaaatcc tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag
    28621 cactcaaggt atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg
    28681 gcttggaacc accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag
    28741 agttgatctc agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac
    28801 ggtagacagg ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta
    28861 cagttggcag ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac
    28921 ggttatacag acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat
    28981 tataggtttc gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac
    29041 ctgttagcca agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat
    29101 agtagtaaac gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg
    29161 attctttgta cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca
    29221 catattccga agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata
    29281 gattgtaatt aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt
    29341 gagcaatcat gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg
    29401 caatactatc aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc
    29461 cagtatagtt ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat
    29521 ctgtcaattg gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg
    29581 tactgtcttc ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg
    29641 tattttctga gacagtagct gactgcaaag tactttctgt atcgttttca attcctgttt
    29701 ctgaagaact cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg
    29761 tgttggcaac tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca
    29821 tcttttttct cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa
    29881 aagcaagtca atgtccatat ttaaataaca tttgtttctt tttaataaat ttgctttttc
    29941 tgcataaaaa gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg
    30001 tttgaataac tatttaacaa gtaattctct ctggatatta tccgttatgg attatacttc
    30061 ctgtgtcgtc ggacggataa tcatgttgct gacagacatc cggtctggcg tttctatcgc
    30121 aaatgctaca gcagaagcta tatcttcaga tgttaatccc cattcttttt gcgcttcatg
    30181 aagttcaaga gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc
    30241 tggagagatg atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat
    30301 gattgcacgg acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg
    30361 tcccgccaca gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag
    30421 tacggcggaa attccattta acacacccat gatgttgata tcaagcatat tttgccattc
    30481 atcacggtga ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac
    30541 atcgattcga ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc
    30601 tcttacatct gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt
    30661 taggcgatct tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt
    30721 cgttgttgct tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa
    30781 atcattcatt ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt
    30841 atttcatctt actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc
    30901 tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc
    30961 attctcgcca atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt
    31021 cttgatgtgg attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt
    31081 caccgtgatg aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc
    31141 taagtcaacg aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt
    31201 gccgaaatct gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat
    31261 gatattttct agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc
    31321 taaaggctct tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac
    31381 tcgaatctct ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt
    31441 tatccttact ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta
    31501 ttccttttaa ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat
    31561 tcgtatactg acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa
    31621 atctctgcat atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact
    31681 tggtgtgaca cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata
    31741 tctttattca aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac
    31801 aaatgacgca atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc
    31861 tttcatttct gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt
    31921 atatagcggg aatgcagatg atatcataga caaactggat agcggattac ttgattttgg
    31981 actcgtaatc gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga
    32041 tcgttgggga cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc
    32101 gcaggaactg aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact
    32161 agctgaatgg ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt
    32221 gtacaatgct tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat
    32281 catcaatact tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc
    32341 actgaatctc atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga
    32401 tgtcctaacg gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac
    32461 aacgaataag ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc
    32521 ttccttttat ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa
    32581 acataactgt ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc
    32641 atactaggca tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat
    32701 ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt
    32761 gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga
    32821 caggaaaaag tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt
    32881 tttgatcgtg atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa
    32941 aatttactta atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat
    33001 gcggaaactg ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac
    33061 attgcttttt ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga
    33121 gaaatcgaag tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg
    33181 acaaaagagc ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt
    33241 cacagcggac gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta
    33301 tctgacatca tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt
    33361 tcactgaatt attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg
    33421 caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa
    33481 agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc
    33541 acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta
    33601 caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg
    33661 gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg
    33721 gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta
    33781 acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag
    33841 tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg
    33901 ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat
    33961 cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc
    34021 tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc
    34081 gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg
    34141 tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct
    34201 ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc
    34261 aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt
    34321 tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca
    34381 tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca
    34441 aagcaaaaac agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa
    34501 cagaaccact tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa
    34561 ttttcactgg gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct
    34621 ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat
    34681 tgatgttgat cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg
    34741 ctatcaaaaa attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg
    34801 atactgcaac gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg
    34861 cattaggcgg agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc
    34921 aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta
    34981 tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg
    35041 cagatgagat ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg
    35101 tggttaaaac agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa
    35161 atgggaaact gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg
    35221 gtgacggcta tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga
    35281 tcacaaacat tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag
    35341 tcttgcttca tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg
    35401 taaaagaagg tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta
    35461 ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc
    35521 aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt
    35581 ccaacaaagc ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct
    35641 gagacaaaaa tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact
    35701 ccgcggtaat aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc
    35761 ttattacttg atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag
    35821 tttatcggta tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    35881 cttcatactc aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa
    35941 tatttctttt aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc
    36001 atcagaggaa atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc
    36061 agctgcttct tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc
    36121 ttcaataatt tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata
    36181 gtcagttaga ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt
    36241 tgctgcagct tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc
    36301 ataaacgaat tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata
    36361 ccaattattc cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa
    36421 ttggtgatct ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc
    36481 gatataagat accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg
    36541 atttcctgga acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat
    36601 tgagtctaaa tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc
    36661 tcctttttct tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa
    36721 gaattccagc gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt
    36781 tgccactccc tattcttttt atttgttaag atttacagct gtacatttat aaattacacc
    36841 tttatcataa agagtgtggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga
    36901 tgaagtattc tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc
    36961 aagatacccg atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa
    37021 atgtacgtaa aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa
    37081 aaccgcagcc ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca
    37141 gcatcgaagg taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg
    37201 ttggtgcgaa tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag
    37261 aagaagaaat ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa
    37321 aagaaaaaag accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt
    37381 gcctgcattt ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa
    37441 gccttgtcat gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt
    37501 cttgataaac ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat
    37561 ggaaagctat ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc
    37621 tctatattat catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt
    37681 ggtaactccc atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc
    37741 tcttaaaaca tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc
    37801 tgctaaagcg attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt
    37861 tggaacatcc tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc
    37921 gttgactgta aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt
    37981 accgataact ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt
    38041 gctactcttc aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct
    38101 atactttgcc atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa
    38161 gggaaatgac acgattacgc tcttttttct agggatattt ttatttttta actatttttt
    38221 tgctttcact gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt
    38281 aaaagagatc tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata
    38341 gattttcccc caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc
    38401 ccaagtgtgt tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt
    38461 agcaaagcca atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc
    38521 ataagaaacg ttgattactt gatttggata taaatctcct aacttatgca tctcttccct
    38581 ttgagtctcc gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt
    38641 gacaggaact tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa
    38701 ttccatcgat tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg
    38761 tatgccgtat ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga
    38821 aaaaccttcg actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc
    38881 taggagatcg cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat
    38941 ccctgcaata aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc
    39001 tattatttta taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt
    39061 cagatcttta agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa
    39121 agtcaattac ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat
    39181 ctaaagaaaa ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat
    39241 gttttacctt tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa
    39301 aaatatcttt tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg
    39361 ttcaaactgc tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt
    39421 atcaattgga acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa
    39481 aaacaaaaaa agttccatct ttaaaacacg ctattctttt actttttctc acattctcta
    39541 agaaaagaaa aggagaatga atttttgaca attcgagcaa aagtgattat caaatatgta
    39601 aaatcagcta cttttttctt aaacgtttct ttatactaaa tataaataag tgtttcatga
    39661 aaggagaact agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt
    39721 tacagctatc atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc
    39781 gcaacagttt ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac
    39841 atgacttgtg attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc
    39901 ttattcgctt acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg
    39961 tcccttgttt cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata
    40021 gaacatccgc aatttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat
    40081 tcagataaat taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac
    40141 aggaaaacag cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg
    40201 ctcattcagc aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact
    40261 ctcgaaacat tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt
    40321 tattatttat ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc
    40381 tcttggttcg tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactccata
    40441 aaaatcctga aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat
    40501 aaaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca agtaaacgat
    40561 aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac acgaatcatg
    40621 caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg actattaggt
    40681 acgcgttttg ttttataagt catgacaaat ttccttcgtt gctgggtaga ttttccggca
    40741 ttgttgatag attttatgat acttttctac attttgtaag agaggttcca ctgcatctgt
    40801 atattggata aaattctgac aacaactttc aggtgtgtcg aaccactcta atctttaaag
    40861 ataaatatta aaataacgat taataatatt aacagtcttt atttttcacc accaaaaacc
    40921 tatctttgca tatactaaat agacgtatat acacagatag gtttgtcttg tgaaaaataa
    40981 tgattatcct taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc aaaaaatatg
    41041 atccttattg aaattactgg ctatttatat tccaacgact tcttgtgtta atttttgtta
    41101 tctcatagta aattatgttt cattttcact taatttagtt gtcacgtgtt cgtcaattcg
    41161 taaaaacggt aaatatataa acacacttgc ggctataatt aatatttgta aaacagctgc
    41221 tcgccagtct ccggctgttg ctaaaaatcc cgatataact ggtggcgtag tccaaggaat
    41281 ttgcactact acatgattaa ttaatcccac agcagttgca aaatatgcta ctgtcaaaga
    41341 aaaaatcggt gcaagcacaa agggaataat cagactgata ttaaatacaa taggcaatcc
    41401 aaaaataata ggttcgctta tattgaatat agcgggagtt accgataatt tagcaaagtc
    41461 cttataatct tttctacgac tgaagataaa tattgcaatc aataaagcaa tcgtatttcc
    41521 cgatccaccc attaccgcaa aggtatcttt aaaggccata ttgataatat gtggaatttg
    41581 ctctttgttt gcataagctg ccatattttc ttgcatattc tgaatcaaaa acggatctaa
    41641 caatgaacct gatattactg cttgatggat accaaatcca aaaaatagat tagcaataga
    41701 tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta cttaatggct ctgtaactat
    41761 cgtggtaatt agcttattta tatccatatt aaacaattga atgattataa atgacaataa
    41821 agcaaacgag gtaatcgtaa taatgattgg gattaataca ttaaaagact ttaaaacagc
    41881 gg
//
LOCUS       DAZUKA010000007        37058 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000007 DAZUKA010000000
VERSION     DAZUKA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.007"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1379)
                     /locus_tag="ACG8PN_000305"
     CDS             complement(15..1379)
                     /locus_tag="ACG8PN_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4860488.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1376..2935)
                     /locus_tag="ACG8PN_000306"
     CDS             complement(1376..2935)
                     /locus_tag="ACG8PN_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860489.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2991..5471)
                     /locus_tag="ACG8PN_000307"
     CDS             complement(2991..5471)
                     /locus_tag="ACG8PN_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860490.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5455..5790)
                     /locus_tag="ACG8PN_000308"
     CDS             complement(5455..5790)
                     /locus_tag="ACG8PN_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860491.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5804..6838)
                     /locus_tag="ACG8PN_000309"
     CDS             complement(5804..6838)
                     /locus_tag="ACG8PN_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4860492.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6928..7608)
                     /locus_tag="ACG8PN_000310"
     CDS             complement(6928..7608)
                     /locus_tag="ACG8PN_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4860493.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7892..8995)
                     /locus_tag="ACG8PN_000311"
     CDS             complement(7892..8995)
                     /locus_tag="ACG8PN_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4860494.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9217..9687)
                     /locus_tag="ACG8PN_000312"
     CDS             complement(9217..9687)
                     /locus_tag="ACG8PN_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4860495.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10020..11660
                     /locus_tag="ACG8PN_000313"
     CDS             10020..11660
                     /locus_tag="ACG8PN_000313"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4860496.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11814..13136
                     /locus_tag="ACG8PN_000314"
     CDS             11814..13136
                     /locus_tag="ACG8PN_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4860497.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13198..13629)
                     /locus_tag="ACG8PN_000315"
     CDS             complement(13198..13629)
                     /locus_tag="ACG8PN_000315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4860498.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13693..14529)
                     /locus_tag="ACG8PN_000316"
     CDS             complement(13693..14529)
                     /locus_tag="ACG8PN_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4860499.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15271..15897)
                     /locus_tag="ACG8PN_000317"
     CDS             complement(15271..15897)
                     /locus_tag="ACG8PN_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860500.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16192..16527)
                     /locus_tag="ACG8PN_000318"
     CDS             complement(16192..16527)
                     /locus_tag="ACG8PN_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4860501.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16514..16798)
                     /locus_tag="ACG8PN_000319"
     CDS             complement(16514..16798)
                     /locus_tag="ACG8PN_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4860502.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(16854..18377)
                     /locus_tag="ACG8PN_000320"
     CDS             complement(16854..18377)
                     /locus_tag="ACG8PN_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4860503.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18370..19545)
                     /locus_tag="ACG8PN_000321"
     CDS             complement(18370..19545)
                     /locus_tag="ACG8PN_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4860504.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(19549..19734)
                     /locus_tag="ACG8PN_000322"
     CDS             complement(19549..19734)
                     /locus_tag="ACG8PN_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860505.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(19700..21394)
                     /locus_tag="ACG8PN_000323"
     CDS             complement(19700..21394)
                     /locus_tag="ACG8PN_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4860506.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21391..21864)
                     /locus_tag="ACG8PN_000324"
     CDS             complement(21391..21864)
                     /locus_tag="ACG8PN_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4860507.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(21933..22088)
                     /locus_tag="ACG8PN_000325"
     CDS             complement(21933..22088)
                     /locus_tag="ACG8PN_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS4860508.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22222..22602)
                     /locus_tag="ACG8PN_000326"
     CDS             complement(22222..22602)
                     /locus_tag="ACG8PN_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4860509.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(22606..22821)
                     /locus_tag="ACG8PN_000327"
     CDS             complement(22606..22821)
                     /locus_tag="ACG8PN_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860510.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(22824..23231)
                     /locus_tag="ACG8PN_000328"
     CDS             complement(22824..23231)
                     /locus_tag="ACG8PN_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS4860511.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23497..24972)
                     /locus_tag="ACG8PN_000329"
     CDS             complement(23497..24972)
                     /locus_tag="ACG8PN_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS4860512.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(24962..25810)
                     /locus_tag="ACG8PN_000330"
     CDS             complement(24962..25810)
                     /locus_tag="ACG8PN_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS4860513.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(25847..26209)
                     /locus_tag="ACG8PN_000331"
     CDS             complement(25847..26209)
                     /locus_tag="ACG8PN_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860514.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26210..26368)
                     /locus_tag="ACG8PN_000332"
     CDS             complement(26210..26368)
                     /locus_tag="ACG8PN_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860515.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(26509..27133)
                     /locus_tag="ACG8PN_000333"
                     /pseudo
     CDS             complement(26509..27133)
                     /locus_tag="ACG8PN_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27130..27441)
                     /locus_tag="ACG8PN_000334"
     CDS             complement(27130..27441)
                     /locus_tag="ACG8PN_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860516.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27485..27778)
                     /locus_tag="ACG8PN_000335"
     CDS             complement(27485..27778)
                     /locus_tag="ACG8PN_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860517.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            27972..28619
                     /locus_tag="ACG8PN_000336"
     CDS             27972..28619
                     /locus_tag="ACG8PN_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4860518.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            28680..29825
                     /locus_tag="ACG8PN_000337"
     CDS             28680..29825
                     /locus_tag="ACG8PN_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4860519.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(29906..30211)
                     /locus_tag="ACG8PN_000338"
     CDS             complement(29906..30211)
                     /locus_tag="ACG8PN_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4860520.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30236..30667)
                     /gene="ruvX"
                     /locus_tag="ACG8PN_000339"
     CDS             complement(30236..30667)
                     /gene="ruvX"
                     /locus_tag="ACG8PN_000339"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4860521.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(30664..30930)
                     /locus_tag="ACG8PN_000340"
     CDS             complement(30664..30930)
                     /locus_tag="ACG8PN_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4860522.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31185..31910)
                     /locus_tag="ACG8PN_000341"
     CDS             complement(31185..31910)
                     /locus_tag="ACG8PN_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4860523.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32271..32341)
                     /locus_tag="ACG8PN_000342"
     tRNA            complement(32271..32341)
                     /locus_tag="ACG8PN_000342"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32307..32309),aa:Gly,seq:ccc)
     gene            32458..33210
                     /locus_tag="ACG8PN_000343"
     CDS             32458..33210
                     /locus_tag="ACG8PN_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4860524.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33226..33705
                     /locus_tag="ACG8PN_000344"
     CDS             33226..33705
                     /locus_tag="ACG8PN_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4860525.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(33956..35536)
                     /locus_tag="ACG8PN_000345"
     CDS             complement(33956..35536)
                     /locus_tag="ACG8PN_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4860526.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35529..36272)
                     /locus_tag="ACG8PN_000346"
     CDS             complement(35529..36272)
                     /locus_tag="ACG8PN_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860527.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36448..>37058)
                     /locus_tag="ACG8PN_000347"
     CDS             complement(36448..>37058)
                     /locus_tag="ACG8PN_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4860528.1"
                     /translation="SDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAIT
                     VINRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYI
                     HYDEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTEN
                     ITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT        10823 a         7500 c         6316 g        12419 t
ORIGIN      
        1 gaccgtgata tttctcacag tcttttatct actctattgc tatttgctct cttagctagt
       61 actttaaaag ctaactcatt gaggtagtga aaacgagagg tgtattcaga cagaaatcca
      121 aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg
      181 ttcaaatctg ataataagaa ttcttggctt atcagctgtc cgccaattat ttctaatgta
      241 tccggatggt agatcagctt tgcatttact gttttgtttt cttcgtcgta ttgtggaaat
      301 agatcaaatt ctcctgaaca cgaaccggta ttttgttcca agaatggcgc ttcatcttct
      361 gtcagcccta cagttcctaa aaagtagtct ataatgactg ctgctgttat cttttgtctg
      421 aggtcgtaag cggttggctg accactcaaa gttagcgcta ctgctcttgc catatgtaca
      481 gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt tgtcaaaact gactgggacg
      541 atatctccaa tcgcatagat gtccggatca cttgtctgca gatattcatt gactatcaca
      601 gtctcatcct catacaaagc cactttttct tttaacaatg tcgtattagg acgagtttgg
      661 gcagaaaaaa tgaccatatc tgcctttatt gtttgagaca gaagttgtac ggatactact
      721 ttctcttctg attttttcca atcgatcaag aattcatttg gataaaaatt tatttgctta
      781 gtttgcagct gattttgaaa actgttgatc atatccttat cgaaatatct tgataaaaca
      841 ttcggcatat aatcaactag atgaataatg tatcctttat ttctcaaagc atctgctaat
      901 tccaatccaa tgtaaccagc accgaagatc actacttctt ttgcttcttt taaagcggca
      961 agtacttgct tagattgcac tagattttta taggtgtaga cattttcagc ttcaatttgt
     1021 ttatacaagg tgacaggaga ggaacctgtc gctacgatta atttatcata ggttatggtc
     1081 tcgttttttc cttctttgga tgcgaggatg agctgatgtt tgtctggatc aagttcagtt
     1141 actctccatt cggtgatcag ttttgcccca ctttcttgaa gagtccgcat gttatgggca
     1201 acttcagcta aactacgtat ctttcctttt aaataaaggt tgattccatt tgaaacatag
     1261 cctagtccat ctttatgatc ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt
     1321 cttagattta gggcagctgt gatgccttca tgggaagcac caatgatcac gattttcatg
     1381 ttttattcat ccttaaattc aatttttcta aataacattc ttttaaaaat tggttcaaat
     1441 aggggaaatc ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
     1501 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
     1561 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
     1621 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
     1681 tgatcagcgt ccgtaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
     1741 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
     1801 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
     1861 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
     1921 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
     1981 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
     2041 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
     2101 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
     2161 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
     2221 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
     2281 atttcaatcg tccttcttct gtaatagcaa aactaaactc tttttgcagc agatctaaaa
     2341 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataggga cgtccaaacc
     2401 aaaataattg gaaataaaaa taacgaattt gactttctgg accggtcagc cgcccgcttt
     2461 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
     2521 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
     2581 acagataaag aagtatctga tattttgtag aatagcgcaa gtaacaatga taataaacat
     2641 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
     2701 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
     2761 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aacctctcct ttttcctcta
     2821 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
     2881 ccaagtccac tccttcaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
     2941 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
     3001 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
     3061 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
     3121 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgc
     3181 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
     3241 ttaaataaag aagtcaatgt acctgtcagt tgccaaggag acgtgcttgg aagccgagta
     3301 tcccgcacat caaaagaaaa atcgcctttt gattcataat aacttgtctt caacggtttg
     3361 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
     3421 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
     3481 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaaa
     3541 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
     3601 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
     3661 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
     3721 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
     3781 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
     3841 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
     3901 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
     3961 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
     4021 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
     4081 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
     4141 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
     4201 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
     4261 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
     4321 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
     4381 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
     4441 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
     4501 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
     4561 gttgaatcac ttggtgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
     4621 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
     4681 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
     4741 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
     4801 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
     4861 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
     4921 tctgtcaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
     4981 gctgataata aacttccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
     5041 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
     5101 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
     5161 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
     5221 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
     5281 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
     5341 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
     5401 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
     5461 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
     5521 ccataattct ataccaatga tcaaataaag aaaacggatc ttttgttcat tcgtctccgg
     5581 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct gttttgatgc
     5641 gccattgact gaaccacttg gatgcggaat tggacttaga tcataaaatg taatcgtacc
     5701 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
     5761 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
     5821 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
     5881 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
     5941 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
     6001 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
     6061 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat gcattagacg
     6121 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
     6181 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
     6241 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
     6301 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
     6361 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
     6421 ttgctgggat tttcacttct ataggtacag ttgtttctgc atgtgctgat acattgattt
     6481 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
     6541 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
     6601 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
     6661 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
     6721 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
     6781 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
     6841 tccaccacat cctttcaaca aaaactgcgg taaaaaatcc ttcacctata gtattttcac
     6901 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
     6961 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
     7021 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
     7081 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
     7141 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
     7201 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
     7261 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
     7321 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcgggaac
     7381 tacatccagt gttaaaggac ctgtctggcc agttgctgga ttttccggat ctgctggctc
     7441 tactggtttt gaaggatcag tagggttaag aggctccacg gtatttgttg gttcagtaaa
     7501 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
     7561 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
     7621 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
     7681 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
     7741 acatgaatat tcttcctttt ttattaagta tgaaaaaata tttcaatttt attttttcgt
     7801 ttcaaattat tctattagtg acatccttga actataaaaa taggagtacg gcatcatctg
     7861 ccgcactcct atttttttcg tcatttgttt cttaaataaa acgattctca cgcaatttta
     7921 tttttttgat atcttctact gtttgtgttc cagctaattg catgaccatt tccaattctg
     7981 ttttaaagaa atcaaacact tgatggactc ctgtacttcc tcctaaagac aatccataaa
     8041 taacgggtct gccaatagca acaagatctg cacctgatgc gattgctttg aaaacatgct
     8101 gtccgcgtcg tacaccgctg tcaaacacga ttggtacacg tttatctact gcttctgcca
     8161 catattgcaa cgaatcaaac gctgctggac caccatctag ttgacggcca ccatgattag
     8221 atacccagat tccgccagca ccagattcta atgaacgata gacatcttct tctgactgga
     8281 cacctttcac atagactggc aaatcagaat gagcagcaat aaattctaca tcttttggac
     8341 taagcttctg tttggaagat ttgtacacag catccatcgt ttggcctact cctgattgat
     8401 aagcctgaac gatcggcatt ggcagtggga aggtaaaccc atttctgcgg tctgtttcac
     8461 ggtttccgcc aactgttgca tccgctgtca atacgattgc ttttgctcca ttacgcttgg
     8521 ccacttcaag aatatccaaa ttgatcccat catctttgct catataaaat tgaaaccatt
     8581 gcggtgcttt ctccccgcct gcttctctta tctcttctaa tgtacaagaa gcgtaagaac
     8641 ttgctgtata gatcgtccca aaatctgcta caccttttgc agaagctttc tccgccttca
     8701 catgagctaa gccatgagca gcaactggtg ccatgatgat tggtgcagtc agcgtttctt
     8761 tgtcaaaatg tgtagttgta tcgggtaatt ctacatcttt taatacatga ggaataatca
     8821 gctgatgatt gaatgcccgt tcattttctt gataagtaaa taagtcgcca gcaccactgc
     8881 tgatgtaacc ataaccacca gctggaatca cttgtgaagc ggctatttct aaatcttcca
     8941 tattgataaa atccactatt ccttcatttg tacctgcttg atacgttttt tccatttcaa
     9001 tgccttccct ctataaaaaa ttgaatgaat aatatgatag cgtttacaca ctttatatta
     9061 ctcttccgta ttgatacagg caaataatct gtttacattt cagtcattcc agttgttact
     9121 actctgaaag aaatgtttag aacacaaata gataaaaaac gatgggactt tgacaaaagt
     9181 cgtgtcaaag tcccttttac gaaatactgt ggatgcctat tgaaataagt gcgggttgtt
     9241 tctttgtatt tcaatcgttt cttgccattc ttcgtttaat tttggattac cgttttcttc
     9301 cttgaacgca taaggtccca atccaaaaag ctcagttagt atttgattca cgattccata
     9361 tagctcggtg tgatcggaca catgattatt gaagatcatc tcattatcac cacgtttgaa
     9421 tctcacattc gggctctcat gatgaacctc tatgcagttt tccccttttt ttaactgacg
     9481 gatttctgaa aacatctcat aaatatcttc tccaataaaa ccttcagcaa gcgccagact
     9541 gtccacaaga ccgcctactg tcaactcaga gattatcgct ttgttggcac caagctttaa
     9601 tgtatagctt gttgcttcta ctacgccgca tgcattgcaa ccaccttctg atagttcgct
     9661 ttttctgatc aattgaactt ctttcatttt ctgttcctcc tcttagtttt ctacttatgt
     9721 attttttatc acctgacgta agtgtatttt atcgtcacga tcacgtgatt tattcttcag
     9781 agattctatt tatctatcta acatatcctt tattcttcta cttttctttc tttagcatat
     9841 aagagatacg catagtaatc aagcaaggat tgttgatata aaaagaacaa ataccgctat
     9901 tttgatttct tttgactcta ttactcatat aaataaaatt tattatacct gctctttttg
     9961 actatatcct catctgactc ctatacttcg tttgtattaa aacttaggag gaatcattca
    10021 tgcttacagg gctaaactta ttaaataatc cttttctaaa taaaggaaca gcttttacaa
    10081 aagaagaacg ggcaaaatac ggaatcactg gtatgcttcc aagtactgtc caaacattgg
    10141 aacaacaatc tgtccaagct tacggtcaat acttaagcaa acagaccgat ttggaaaaac
    10201 gtatcttctt aatgaatcta ttcaatacga accggacatt gttttataaa ttgatgggcg
    10261 aacatttagt tgaattcatg cctgtcgttt atgatcctgt cgttgcggat tcaatcgaac
    10321 aatacaacga aattttcttg aaaccacaag atgctgcatt tctttcaatc gacgaaccag
    10381 aaaatatcaa agcaagtttg aaaaacgcag cagatggacg cgacattcgt ttgatcgttg
    10441 tgacagatgc agaaggaatc ttaggtatgg gtgactgggg tgtgaacggt gttgacatcg
    10501 ctatcggtaa attgatggtc tacactgctg cggctggtat caatcctgca caagtcttgc
    10561 ctgtatcaat cgacgctggt actaataacg aagaattatt gaataatcca ttatacttag
    10621 ggaatcgtca tgctcgtgta gaaggcgaaa cttactacga attcattgat caattcgttc
    10681 aatcagctac tgaattattc ccagaattgt tattacattg ggaagatttt ggacgaggaa
    10741 atgctgcaac gatcttagaa aaatacgaag ataaaatcac tactttcaat gatgatatcc
    10801 aaggcacagg aatcgttgta ctagctggtg tattaggtgg attgaatata tcaggtgagc
    10861 aattgaaaga tcaaacgatc ttgacatttg gtgctggtac tgctggtgta ggtatcgcaa
    10921 acatcctttt agatgaaatg atccgtcaag gtgtacctga agaagaagca cgccaacatt
    10981 tctatcaagt agataaacag ggtttattgt ttgaagatac aaaaggattg actcctggtc
    11041 aaatcccatt cgctcgtaaa cgctcagaat tcactaatgc tgatgaattg acaaacttag
    11101 aagcagttgt aaaagcaatc catccaacga tcatgatcgg tacttctacc caacctggag
    11161 cattcactga agaaatcgtg aaagaaatgg cggctcatac cccacgtcca atcatcatgc
    11221 ctttgtctaa tccaacaaaa ttggcagaag caaaagcaaa agatttgatt gaatggacgg
    11281 acggaaaagc tttagttgga acaggtatcc ctgctgctga tgtggaatat aacggtgtaa
    11341 cttaccaaat cggtcaagca aataatgcgt tgatgtaccc aggtttaggg cttggtttga
    11401 ttgcttcaac agctacacga gtaaatgctg aaatcatttc tcaagctagt cgagcattag
    11461 gcggaatcgt tgatgtaaca aaaccaggag cagctatctt gcctccagta gcaaaaatca
    11521 ctgaattctc tcaaacgatt gctgaaactg tcgcaaaatc agttgttgca caaaaattga
    11581 atcgtgaaga aatcacggat atcaaagaag ctgttgaatc tgctaaatgg gttcctgaat
    11641 ataaatcttt agaagattaa gattgttata gtaaaagaac atacagaaac tttgtatgac
    11701 gtctaaaaag aaaaaagcga ctgcggcaaa acagcagcca gccaactact gctcttttgc
    11761 cgcttctctt tggctttttt catacgatac agaataagaa aggatctgta aaaatggcac
    11821 aaaaaacaac aaccgcaact gcagttgagg aaaagaaaaa agaagggttt tggtcaacaa
    11881 aaattaccgg cgtaagtttg cccctttatt tgatcatggt ccttgtcctg atcattgcaa
    11941 tggctttagg aaaattaccg acgaatatga ttggcccaat cgctgttttg gtcattttcg
    12001 gtaatttatt tcattttatc ggaacaaaaa tccctatcgt caaaagttat ttaggtggtg
    12061 gctccgtttt tgcgatcttt gcctctgccg cgattgctac atttgggatc ttacctgagt
    12121 atgtagtaaa atccacagaa aactttgtca gcaatatggg ctttttagac ttttatattg
    12181 ctgccttgat tactggtagc atccttggta tgaaccgtaa tctattgatg aaagcttctg
    12241 ttcgttttat tcccgttgca cttatttcaa tggtcgtctc cttttttgcc gtaggactcg
    12301 taggtatgct tatcggaaat ggttttgcaa attccgtttt atatatctca ttgcccgcaa
    12361 tggctggtgg tgttggagcc ggagctgtac ctttatcaac gatttatgca aatgttttag
    12421 gtacggacgc ttcttctatc atttcccgcc tgattcctgc gacagcaatg acaaatgtcc
    12481 tagctatcat tggtgcagcg ttagttgctc gtgcgggaca atcaatgcca aaattcaacg
    12541 gtaatggtaa attaatggaa aaaggcgacc ttggcgatgg aaaacctaga aatgtaaaac
    12601 cttccattcc acaattaggt gtcggtttga tggtatcatt gactttcttc atcttaggac
    12661 aaatctgtaa ctattttgtt cctaaagtcc atgcttacgc attcatgatc attatcgttg
    12721 ttatctgtaa aatcgcaaac ctattaccag aatattacga agatgcagct atcatgttca
    12781 acaacttgat tgtcaaaaac ttgacagcag ctgttcttgc cggtatcggt attgcgttat
    12841 tgaatctaaa tgtattggct tctgctttga catggcaatt tgttgtctta tgtttgacaa
    12901 gtgttatcgc gatttcagta gtttcaggat ttgtcggtcg tttgtttggt ttatatccaa
    12961 tcgaatcaac tattactgct ggcttatgca acaactcgat gggtggtaca ggaaatgtag
    13021 ctgtcttatc tgcagctaat cggatggaac ttattgcttt tgctcaaatg ggcaatcggc
    13081 ttggtggtgc catcgttttg atcatctcag gtttcttgat gcaattactt tcttagtcaa
    13141 aaaaaatacc tgatcatcaa gtatcggctt gatgaccagg tattttttga taaaaattta
    13201 caggattttc aaaaatcttt tttgcatttt atcggaatga taaccgatgc gttcataaaa
    13261 tcggtgtgct tctactctgt attctgcaga attaagtctt attgctgata ttcctcgtct
    13321 tttcgcttct tctacgccag tcatcaaggc ctttccgatt cctctttttt cagcatgttt
    13381 agacacagca agtgccataa tattgaacat cggatcgctg aaaatcgatt cataggactc
    13441 cgcttgtaca tagccagctg cttttttaga agtaggatct tcatagacca agaaaaaatt
    13501 tttttctggc tccttcaaca gtttcttcat ttgtctcacc gtaacggtta atggtacatc
    13561 atatcctaat tgttccgcat tgatttctcg tagtgcttct gcatctccta aacgcatttc
    13621 ccgcaacatc caaatcacca gccaattttt atttttttaa atgaattttt ttatcacagt
    13681 atactccttt tttcatttag aacaatatgt ttcagtgatt ttgacttgcg ttagtctaga
    13741 ttctcctaat gtttgtaaga tgacgaaagc attgtaaaga ttgatcaaaa gaaccacgag
    13801 gacaattact tgaaaaataa aggtcacttt ttttggagac aaaaccctgc taaatcttgc
    13861 tccccacaaa cctccaataa cagcagcggg gataacataa aacaacatag ataaatcaaa
    13921 gcgcataaag ctacttgtta aagcaatcgt taccaatttt gccagttgag aaaagaaaat
    13981 cgtagataga gaatatagtg ttgcttcttt tattggcaat gcaaacatca gcatcaacaa
    14041 agaaacattg actggtccac caccaatacc taaaaaacta gctaaaaagc ccagaacccc
    14101 cccacatata aggtaccaag tccatgacgt caaatgaaat tttggctgat gatgcttggt
    14161 gtagaagaac gcaaaaacca aggtcaatac cgtcaaaaaa atttggatca gctgcacatg
    14221 cttctcgttt tcaaataact gtaaaaaaac ttcgaataca atgttgccga ctactcctcc
    14281 aacaacagct ccactagata cccaaccgac aatctgccaa ttcagtgatt tcccgcttga
    14341 tacttgtcgc acagtcgaaa caatggacat cgtgaatacc gcaactgttg agtaaaaaga
    14401 aatccctgca acagaatggg cgccgataag atcaagaatc ggtttgatca acacgcctcc
    14461 acccatcccg gaaatagcgc caactgtgtt cgccagaacg atcaccacaa aataaatcag
    14521 accaatcatc ttgtatttcc tttctcttgt ttatatcttt tagcgattgt tactcttttc
    14581 ttttattata cagtaaaaga aacaaacgag gcttcgaaag cttttctact ttttaaggaa
    14641 atttagtgct aaacgaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    14701 gccaaacaaa aatagctcct cataattttc gtttggttga acttattacc gcagatgcaa
    14761 gcttttgaac accgtttaag aaagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    14821 gaacaatttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag
    14881 ttggcaggag aacaccgttt aaggaagaga ctgtgaagtc aacgttttga cctgattatt
    14941 agagaaacaa atggcaaaat agttcttcat atttcgccgt ttttcgagcg aatgccgagg
    15001 ggcagctgat tgaacatcgt ttatttggaa aaagggattg tgacaagacg tttgtcacaa
    15061 tccctttttt tatatcttat taattcattc ttttgttcta ttcttcttct tccgccatga
    15121 atgagaaacc catatcaaag aactataaag gttatcaaaa cagcgtttat gactaaatga
    15181 attagagtta tctatcacta tttttcaagc gatttaaacc aaatggcata caatatggca
    15241 tacaaaaact tcaataagcc gtgggggttt ttaaatttct tcttgttctt taaaatcaat
    15301 gagtggagct acataatttg aatccacttt ttcaaacgtc actttttgca tcgacgaatg
    15361 ttttctttcc caagataacg taaatagatt tgactctgga cttataaagt ctacttcacc
    15421 ttctagtatt actttcttat gcaaatattc ctctaataaa tatagacttg ctaaagcata
    15481 caacacattt tccaaatttg cttccttata atttgaagaa cgaccatgtt ttactctgtt
    15541 atatattttc caccaatctg gtgtatcttc aggccaccca tctccaaaag gttttagtga
    15601 agtcaatgtt aaagaattca taaccctaac accattatcc aaacacatta attcgctaga
    15661 tttaataaca gctttaaaat cagttatgct tttattgcta ctgctatcaa atccgcaaag
    15721 ttctttgaaa ataatctcac actccgaacc aatcgctagt agtagttttt gatattcaat
    15781 agagaatgtt ttgaaattat tctcatcgag catcacaaaa tctgtggtat taataaactc
    15841 attttctaaa tgtagataat acttccaata aaaatttgta aactctgctt tgtccattaa
    15901 atcacctctt ttgaattata acaaaaaaaa cacgtccaca acagacgtgt tctttgattt
    15961 ctacacaatc agcttgccaa aaccatccaa tcagtcctag catgcctgta ttaaaattga
    16021 acgttcatca tctatcatct ttcttagtcc agcatgtttg caaccacacc tacaattttc
    16081 agtccaacaa tagatttcga ctagctaagt cttgcaaaat tcaggcagct aacccttcat
    16141 ttactacctt aattatacta aatacccaca acgtttacaa gtgtaatcat tctaagaaat
    16201 ctgtttagct atgatcgttg tataatcttt aaacgccgtt ccttcattga ttgaaataat
    16261 tttataactc ttgtttttcc aagctacttt catatcgttt gttagttcat aatcttgatc
    16321 gtatcgaata ataaacgtca acgttccttc aagaatggta cctacactag caagcacgtc
    16381 ttttagcatt tgtgaacgca cattagccca acattcaaat acagtttctt cactatccac
    16441 catctgccca tcttcatctt ttacccgttt tgtcttttta aacttgactc tctcagtcaa
    16501 ttcatttaca ttcttaatga ttggcataat aagcacctct catttgttgt actaatgctt
    16561 gtaccgttac tggtatccgt tcgctgctgg cttcttgacg gttcaaatac caatgttgag
    16621 taagtaatga caccgccaaa tcaaattgct gataattatc catatcttta tccgttgcgc
    16681 tactatcaat agcacttttt atatattcgc ctgctgtatc aatcaattgt tgaataaagt
    16741 catcatcaat agtgtgatcg atccgcatac ttttttttat gctatccaaa attaccattt
    16801 tatctctcct aatgaaaaaa aggcagtggg ataaaccgcc gcccaatatt tttctatact
    16861 tttggtgtta caactggcgt aaactcaata tacactgaag cattttgatc aattttctta
    16921 taatcgttgc gaacgatcac tgcaagtcct tgtgagtagt aatcgaattt ttcccattga
    16981 gtcgttactt gatttctacg agcaacaaag atagattgtg caatatcccc cataatcata
    17041 gggaatgttc cagctttagg attggcaaac aatgtatctg caatcaatac tactggcata
    17101 ccgaataatg atttaccact aggtgctgtc acatcgggtt gtaaaatgta acgtccatct
    17161 gaatctttca atgtatctag gtggtaatat ccgctttggt ttagaattac cattttattt
    17221 aatgctgggt ctaatgccac attgtatagt tgtttcaaat cgtccaacgt agcagccgtt
    17281 ttcttagtga atgtttttaa taaatccatg atatgcttat tgtcagtgtt ttctaccaat
    17341 ttcgctaact gatctttgac ctcttgtaca atattcactg ctgaatcttc cacaacctca
    17401 tttgataagg caatcttacc agcacgagtt tctactttat aatcaactga agtaaacatt
    17461 tcagcgtcaa tatcaccaat ttcagcaagt tcagcttttg ttgctaacac tgcttgttgg
    17521 ttagttgcta ctggatattt accttgtcca tttgatactg tttttactgt tgcatattga
    17581 gctaagttat aatttgaacg tttcaaatca aagacttcac cgattacttc ttcgggaaca
    17641 actgccgctg catttacagt agtgactcca tcacgcactt cgccttgtga acggatatat
    17701 tcttcatacc cacgcaattc tgttttttcg ttatcgatta aagttttttc catgttatta
    17761 ctctcctttt gtgctttaga agtggattct aagctattgt ttgtttgttt tgcttggttg
    17821 ttactcataa acgattcgta agaacgctta tttacttgga catttgatga atcataagcg
    17881 ggaacggtca cgacactaat ttcattaagt gctttgattt tatctattga tcgttcaatt
    17941 gtgccatctt cttttttgtc gaatgaatct aatccaagca cgaacccaaa ggacatagaa
    18001 tcaacaaccc cttttgagat attctcatac acatcatttg aataggtcgt gtcggttagc
    18061 tctgcttcaa aatgtaatcc tgtatcatcg acattcaatt tcaacgtacc tgctttgaca
    18121 ctagctagtg gctttgaata atcgtgtcca tataataaga acacatttga taaatccacc
    18181 tcttttaatg cttcgggtgt aatcacttct acaaagccgc ctaaatcttt tgacggttgc
    18241 ccgaatttca acgcatagcc tgaaatagtt ttgccgtctg ttggttgttt ttcattttct
    18301 ttctctactt tgggagaatc ggctgttagc tcagcttctt ctgtcagccg tttttccttt
    18361 tcttcttcat tattcaactg gtgtaactcc ttcctgttct tttaattgat atttctccaa
    18421 cgtatctaaa tacgtaaagt tcaaacttgc tagtaaacga tcgccaccat ccatgggggg
    18481 tagccccatt ttcgaccgcc cctcattaat cgtgagaagt gaccctttga cctgatccaa
    18541 tacatttttt acttttgttt caggatcagt ctctagtaac tgatcggaat taaagcggat
    18601 attggtttca agtttcgtat ctaattcact cacaaacaca ttaaagtaat gaatcagcgt
    18661 actttggata tactggagat tactttgaac ggtgctagaa tgttcatttt caacgcctaa
    18721 acgctcaatc ggtacaccaa acgccttggc aatttgtttt gtgttccaat cattggaatt
    18781 gataagcttc aaaacatcgg tgtttatttc taatgttttg tagtccattg tttcatcaag
    18841 aatgatcgtt ctaagagcat tatctccact acttgaaccg ttggcttctt caaacttttc
    18901 acgaattgcg ttctttgctg atccgtctaa atctgactta tgaaccttca aaatacctga
    18961 cccactgaca ccacgagtaa agaaattgtg caatgtgcgg ttgccagctt gttgaatttt
    19021 cagttcatca cgtaacgcat aaagtggtgg taatcctgtt aaaccatctt gggtgaaata
    19081 tttgaagtgc aaaatatcgc tagacttcac acgtcttttt ttatcaccaa tctgataaga
    19141 caacgtacca ttatctaatt gagtaaccgt tacacttgag ttaggtaata agtgaatttc
    19201 ttctaccttt tcaccgttgc gtttaatctc tgcaaagcta ttaccgttta gcaacatatt
    19261 gaccgccaaa gcaaacttta aatgccaacc atccatttct gaattaggtt tctcgtttag
    19321 taacttcacc agttcattat cagcttgtgg catattgttt ttgaccaatt gaatcggact
    19381 tgaagcaata tcactagcaa taattcgaac ggctgcaaac acatcactat ttcttaatgc
    19441 acgaacgctt gtaaagctgg tggtgtaatc atccgattgg attgatacca catgttgtag
    19501 gaaaggatct cccgttgtat tttgtctcgc tttaaaaaat gccatctttt aacctccctt
    19561 ctgttctgta tttagcatga ccgctacccc aataagcact atgcccagcg ctaacaagcc
    19621 atagtagaca tttgttagta aggtgatagc aaccaacaca cacaccaatc caagaagtaa
    19681 aagaacggta tgaatgtaat tagaaagaaa attcttcact tgtataaaat tcattgtctg
    19741 ctttcgctcc ttccgttgtg gtgaagtaat ccattgcaaa cacataggca ttgattaatg
    19801 cagctattgg atcaattttg ttactgttct ttgatttatt aatctgaatg ccgttgttat
    19861 ccgtttttaa aatagcgttg ttcactgcat gggtgagaat cgtattttta ttgtgaataa
    19921 cgttgccttc ataaacttgt tctctaaaag ttcgggtcgg aacgtttaga gtaatcgttc
    19981 cttgtcttac ttctaacatt gggtagttgg ctttttcagc tttactgata agcgaattag
    20041 cgttatacgg gtcataacaa atccccatac attccaaatc attttcttga atcaaatctt
    20101 gaataaactg aaatacttgg tcatagtcca cgattccact ttctaattga gtaatggaac
    20161 actcaccagc tttttctagt tctcgataag gcaaaccgtc gcgcttttct ttgtcttgaa
    20221 gcccatattt ggtggctaca aagctatgtg aatcacaata aagttcgccg ttatctagcg
    20281 gtacgatcca tgaaacgcta gttaaatcat ctgttttaga taaatccact ccgatataaa
    20341 cgggcttgcc tgtaatatct tgtggttcta cttcggttgc ctgccagtct tcagcaatca
    20401 tatagctgtc ctcactcgct tgtctccata aattgaagtt tttcaccaat acagaattaa
    20461 ggttattttg tttcaaagca aggttcacat catcttgaat ggttggaatc attacttttt
    20521 tgatttcttc actttcaaaa atcggattgg ctttgatcca tttctcttga tcgtgaattt
    20581 cttcgggatc gtccaattcc catatcgcta taaagtacct gtcggcttgt tcttcgcctt
    20641 ttagcacacg atctaacaat aagtattcct cgtacatagg aacgtttaaa tcaagcccag
    20701 cggtactaat aacacacaat aagccgttct tttgttgggt cattcctgac ttaatgacgt
    20761 tgtacgtttt acgagttttt gcttcgtgcc actcgtcaag aattgcggtt gttggtgcaa
    20821 acccatctag cgtgctggtt tcactagcca gtgccatagc gaacgaatta gagggcaagt
    20881 gctgaatctt agagttcata attttcagtt gtggtcttaa aaacttacta gacttgacca
    20941 cgtttctaag tgaattagac agcatatcat agcccaattt agcttgtttc aaagcgttac
    21001 ttacaaacaa tacttgcctt gcttctgctg gttcttgttc cataatcaac gcattggcag
    21061 ccatgccact tgcgatatac gttttcccat tcttacggct catactggta aacgctcgat
    21121 tgaatcgcct gtaattccct tctttagtac gccagccata aagactgcct agaatgaatt
    21181 tttgaaatcc taacatcttg attgctttac cgtccgtaga tggtagcaac tcaatgaatt
    21241 taatcgcttt ggtggcttgt tcttcatcga acacataagg gaagctatct gacttagaac
    21301 gctccaaatc gcttatatga cgctcacacg cttggataat tttcttgggt gcaatcagct
    21361 tacctgatag gactttctca atgtaatcat tcatgaattc atcaactctt tgaacgggtc
    21421 ttcgggttca tcatttttaa ccttgttcaa agctagtttt gatcgtgatt ctaaggtcat
    21481 acctagtgag gtggcttgtt ttttcaaatc actcatagct tgtgattgaa cccgaaccgc
    21541 tggattggct ttctttactc cgttttcgca tgtgataaac gtacctgatt ttcttatctc
    21601 ggcttcggct gttttcattc gagcaaaggc taaacaatag gctaccaatg aaccataatc
    21661 tgcttcactc aatggataat ctttttttaa tgttggtacg atacgattcc attcacttct
    21721 agcgctcgct gccatccagt cagggggcgt gatatttatt aatggttgct gcttgaacaa
    21781 ttcttctcta gcgtccacac ggattgcttt ttcttcactt gatatattgc cttttgtatc
    21841 gtgtaatatt ttagggtgtc ccatgttgtc ctcctttcag acaaaagaaa aaggaacgta
    21901 agcaattaag ctcacattcc ttcgtttttc gatcagaatt tttctttatc taatttatct
    21961 atataaatga tttttccgtt ctgcatttga atggtcactt gaccaaaatg aggtaactca
    22021 atggtgcgct gtttccctgt tttatcagta ataacaattg tatttttcaa taaatctcct
    22081 actttcatta tatacttttt cacttgtgat tacaagtgta atcgttattt gtaattaccc
    22141 ttatgccttt tcacaacggt tatttgtacc aaaatgagta ggcatcgatt gtcagtaagt
    22201 ttcacactat gcccccttaa ctcaaagggt actcttttct cgtctttgtc tttcattggc
    22261 tgtcttggaa ttatggtgtg catggcataa agaacgaaaa ttagtttcat ctaaccgtct
    22321 attccaatca tcttttagtt caattttgtg atctacaaca tcagctttcc ttatcaagcc
    22381 ttcttctagg caatcctcac atactggatg atttaaccga tacaagtatg aagcctttcg
    22441 ccatgttcta ctttgataga attgaaaata tttaccttcg ctttgctttc tttcttcacg
    22501 cttaattgcc gtttgctttg ctttatgctt cccacaatac ttctgcctat aatcaatcaa
    22561 tgttttacat ccagcatgat tacattgttt tttaactgcc atagattacc aaccaaagcg
    22621 atctcgttcg tcagaatcat cttgttccca ttttgatttg tattcaatat aaggcgaaac
    22681 tatcttcaag cacgttccgt ctttaaaatt aactttagtg attttatctt cctcaaaact
    22741 aataaagtct acatcttctt gagtgtttga atactcaatg tgtttctcac catcgaagta
    22801 tctaatactt tccatatgca taattattct acctccaata aattagttat caattcatca
    22861 tctgttagta gcaaagccaa gaacttatca tggatgttta aaaacaaatt atcctcattc
    22921 gatgtaagct ctcgttgtct cgctcgatca tataggttaa gcagacaatt aaattcatcc
    22981 attagctcac ctgtatcatc atagttgcca ctatctacca atgatcccac tactagcgat
    23041 tgacaccacg ctactgactc ctgcagccaa tcaggttctt cttccattat ctttaatact
    23101 tctggatcaa atgtggaata atcttctgtt accaatccca cactaaatgc cagcgtgttc
    23161 attagaaacc gatccatttc atctgtatat tgaaagttcg gtgctttgtt tgttgaaata
    23221 atattgagca tttactttcc tccttgattc gtaacacgtg gtaacaccta cgtaacaggt
    23281 acccgttacc actcaatccc aacggggaca agggttctag cgttttggta acaggtaacg
    23341 ggtaaaatcc agtaacatta ttccccagca tattagctaa ctatttcaaa agttttaagg
    23401 aaataaggtg ttaccccgtt accaaattgc tgtatccctt ggggcagtaa gggtaaacta
    23461 gtaacgggct aggtgttact gacccgttac tacccgttac tttttgtact ttccagctcg
    23521 atttttattt tcataggcat ttgacttatt gtaaaatcct cgtcgtttat ttctttttgg
    23581 cagttgcttc cgttcccaac caggaatact gctaataatc aaggcaatct ttttagtttc
    23641 tgcatctgat cgaccattca ataaatcctt tgcttgttta tcaaaaacag cttccaaaat
    23701 atcagcggtg agtacgctat caactaaata aaactcttgt tgcatagtag ctccattata
    23761 gaaatgctgt aaatttttct cattacattt actgtcagga tagttctgat aataagaacg
    23821 tctcgcccac gctggagctt cttcccattt atacgggatt tccatttcca aatatttagt
    23881 gattgcttct ttaattggat tctccatctt ggcatcctct tgatagtctt ttgcaatttc
    23941 taagacttct ttatcttcat cgggactaaa atagattcgt tgtcctttat caaaaagcac
    24001 ctttgcttca gctaacactt gaagaaaata ttcatccttt gttttaaacg gattcatctt
    24061 tggcttattc ttacaaggca aaggataaaa acggcggttt cctgtatcat ctttcaaata
    24121 ctgcccatca ttcgacgttc caataaatac acattgcctt gcccaagcta tgacattccg
    24181 accataagga ggtctaattt tatcgatctt ggcactgatg aagtttttta tctgttcaat
    24241 tttagttttg ttcattgaag ataattctcc aagttcaata attacattgc cttgaagctg
    24301 catataatca tctttctttt cacctagcga atctaatgaa tcggtgaaaa aatcatccgt
    24361 gtaaagtaat gctggaacgg tactcttacc aatcccttgc ggtccatcca aaactggaac
    24421 gatctcaaac tttattcctg gtttatagat acgtgcgact gatccgacta tccaacgttt
    24481 tgttacttct cgtgtgtaaa ggttgtcgtc tacccctaaa aggtcaataa acagtgtttc
    24541 cagtctaggc tgttgatccc atttaacttt ttcgatacgt tcttttactg ggttgaaaac
    24601 attctttcta gccacattta ctactgcttt gtgtattttg ttgtcatcaa aaactacatt
    24661 gtagttttct tcaatatatg ccgttaaggc atcctcaaaa ctgtcttccc attctccagc
    24721 ggtacgattg atccaaggag aatcttttaa taggtgaatg tatccgctaa attcgttata
    24781 agcaattgtt ccagctaaat tgctatcatt ggttaaaatc aattctgcat tcacaacact
    24841 gttactttta atctgatcgt ctttattgca tcttaatcgt gacatccagg ttttcaattc
    24901 aatgatattg tcttcacgct cgctggcttc gggaaagtct aaaatactat ctaatttatg
    24961 cttatgagcc atgagctttc cccctcaaat cttttttaat catcgatcga ataatttgat
    25021 tcacttcttt atcttttagc ggtggttcat tgtacgcatt tgctgtataa atcaattctg
    25081 ctaaagtttg attatctaca ccgtgccata gtaaccaacc gcataactta gttaaatgat
    25141 cgtttctact accttcgcca gcgccttgaa caatcatatc taattttttt gctgtccctg
    25201 tttttgctac gggtcggtat tgagtcttta tttcttgcgg tttttttagc agtaggttca
    25261 ctagccaatc gggagctggt gcaatattat ttagagatac gttttcaata ggttcatatg
    25321 ctctcccaac acttcttgaa ataatcatag ttggcgctat tggaactcct ttagtgataa
    25381 tatcaatccc gcttaaactt ttacctttat catcacgccc gaagatatca gcaaatgcac
    25441 ctgtttcttg ttggataatt acaccatcag gaattttaaa gaagaaatgg attcctttac
    25501 ctggtgtgat ttccgtataa gtagaaggaa atggatcata cattgaagca agttttttat
    25561 aattctcaac gccgtttcgt gtttcagaat gttgatctag gtcaactacc accatttgat
    25621 gatcttcaag cattaaccca atatttctag ttgcatcttc cgtccaccat tcattaattt
    25681 gattagaatc gttagttgca tcattccaac cactagagcc ttttattgga attttagttc
    25741 caggagataa gggataaatt gaccatcccg acttttgtaa ttccgttgct gcttgataat
    25801 tatttgccaa taaacctctc ctcctttttc aaaatactgg tataaatcat tcagacacct
    25861 ccatgagctt caactgctca agttcgtatt tcaacccatc ccgaacaaat tcagcacctt
    25921 ttaattgatt ctgatacttt acccatgtat taaatgtttt cacccttgct tcttgcatat
    25981 cagtaatgtt taaccatgcg gttaaaatct gattttttag ctcgttgatt tctcgacaaa
    26041 ttattttgtg ttctgcatgt tttttgagta catacaaaac tgttagtttt tcattgaatg
    26101 aatttccaaa gtaggttttc ccgatattat tttctagttc agcgatccct tgattaattt
    26161 cctctaattc aaggctatcg agttggttta atagttttgt tttgtacatc tacttatgcc
    26221 ctccagttct taattgatag tcgtactcgt cataagcagt gaagagatat aacgctaatc
    26281 ctgcaaagat agctattttt gcaattaatg gtaataagcc gaaaattccg ccgattaaaa
    26341 tgcttcctat aattaagttt cgtttcataa tggttccctc cgatttttta atatggtata
    26401 atcggaacaa agaatatcgg gttttatccc aattaagttg cctttatcct gtccgccaag
    26461 actgttatgg ataaggcttt ttctttgttc tcgatcatgt taatgctcct aagttgtctg
    26521 caattcagca gaacgattat aaattttctc actcactgaa aactgcaacc catatttttc
    26581 tttaacagtc attaattcta ctgtctcatc taaaataggt tcacgatcaa ccaacatttc
    26641 aggagtcata tctttctttt ttaccatttt ggaatgacca taaatagttg atacagcttt
    26701 attggctatc acattcgctt taatgtagtc tactggtttt ggttcagtaa tagcgtggct
    26761 taatttagtc attgcttctt tttggtgttc tttgtctagc atgcggaata cttgaaagcc
    26821 ttcaagtcct gtggactggc gtagttcttt gatgatgtcg aaaatccagt cttgaaatgc
    26881 ctcggcttct tttttacggc ttttgaatgc catgcggtaa atattttttt tcgttgatga
    26941 tgtttacatc ttgtgttctg cccaatgaat cgatgacctt actagtagta accccatctt
    27001 taggcaaaga gtgaatggct ctagtaacct gtttaagacc taacgcttcc gcaacatctt
    27061 tcgccactgc ccaccattca tcattaatat cgacaaatct tataatgtgt ccgttccaaa
    27121 tttcagtttt cattcaacgt cctccagttc aagccctagc aagctcgcta acaactcaac
    27181 ttgctgatac aaaatttctt gatactcttc aatagaaagt cgttctttaa actctgcttg
    27241 tactaaatcg ttcaagccac gcaatactcc gcttaccgtt ggttcttgtg gtagcaattt
    27301 ttgatagtcc atttgttcgt acaaacattc aaacgtttct tctagcgctt tgccttccaa
    27361 gtttgctaat tctgccgctg tgtcttctgt aagaattgtt gataatactt tttcgattgc
    27421 tttattttgt gtcgattcca ttattatttc ctcctattaa tccctttgcc cccacggctt
    27481 gcgtttattt ttggtattct tccataaagc gatcacattc atcttgtgaa attcttacga
    27541 ctccatcaac tataatcaac tttaaaccgt taggaacata tttctgcatc atagtattgt
    27601 atgaaacgtt catatactta gcagcttgtg ccttgttcat atattttgga aactggtact
    27661 ttttttctaa taatgaaagt aacgttcctt gagtttgctc tacttgttga acaactcgat
    27721 cgtcaataat tttaaaagct ggatcatcaa gcgtgagttt gtcaattttt aattccatat
    27781 ttatcccccc tcgttagcat acatttctat acaaagattt taaaaaaatg caagaggctc
    27841 ttgaatacat ttctatacct cttataaact caatatacca tcataccttt tcatttgcaa
    27901 gaattttaac atactttttt gaacttttat gtttcacaca tatttaaatc gtgatatagt
    27961 agagaaaata tatgaatggt ggtgaaaatg tggctagaaa aaaatacccc gaagcagtgg
    28021 agacaggaca aagaattaga ctggctagaa ttgaaaaggg actctcaatg gaacaacttg
    28081 gaggtaaatt atccccaccg gccagtaaag gtgccgttag taattgggaa aatggctata
    28141 atcttcccaa taacaccagg cttaaacaac tttcaaaggt attagatgta tccactacct
    28201 atctattaaa cggatcttat acattaagag atatgcatat gatgcccgaa attaagcgaa
    28261 atgtgcttga aaagcaattg aaaaaaacta ttgaaaaaaa taactctata attgattcta
    28321 atctttctaa tgctatcaat aacatcgaag aagataacct gactgtctca cagaagattt
    28381 atttaacaaa cgcaataaaa tatcttaaaa caggatctcg aattgaattt cctgacggga
    28441 gtaccccaat tgcttatctc gcagctcttc taaatacgct tttacgatat gactcattaa
    28501 ttaatgattc agaaaaaaat acagatgaga aaaaagagta tcaagaattt tatgaaaatg
    28561 atcttctaaa agagttcaca gctcttgtta ctgagctaaa taaacactat tcaaaataat
    28621 aataaactgc ctcctccaac cgcccccacg gcttgctaca ctatggaggg aaaactatta
    28681 tggcaacatt caagcaatat gaaactaaaa aaggtaaatt ttggctctat gaagcctatc
    28741 ttggaatgaa caaaatgact ggcaaacctg ataaggttcg tagacgaggt tacaaaacaa
    28801 aaaaagaagc ccagcttgcc ttgtcacgct tgcaagtgga ctacgataaa aacggtatta
    28861 aaaaagcgaa caacgaaacg tttaaagaag tttatgactt gtggctagaa acatataaaa
    28921 ccactgtccg agaagccacc ttcatgaaaa ctgaaatcaa gtttagaaag tggattcttc
    28981 caaaatatgg aaatcttcgt gtgaatgaag tcacggtaaa aaaagcccaa caaattgtaa
    29041 atagctgggc tgaaactact gaccaataca aggtacttca ttcaacttct aagcgtattt
    29101 ttagctacgc tatcaatctt gggattattg agactaatcc actagacaac gtattaatgc
    29161 ctaaaagaac caccactaag cataaagaaa aggtcaaagt ttacagtaaa gaacaactga
    29221 tcaccttgtt tgattaccta aacagcaaac ctgaaactta tcgaaacgac tttgataaaa
    29281 cgttactacg ctttctcttt tatagcgggg ttaggatcag tgaagccctt gcattgaatt
    29341 ggtcagacat tgattttcaa gaaaatacgg tcaccatcaa taaaacacta agccaatcca
    29401 aaaatggcta taaaatcagt gatcctaaaa ctgattgtag tgctggggtt cttccactag
    29461 atgaaagaac aatttctata ttaaaaaaat ggcagattaa tcagcggaaa tatatgctga
    29521 cacttggtgt aactgatccg actatgattt tttgtggtat ctataaagaa ctaatcacac
    29581 accacgctat ttatgcaaga ttgattacta tttgtgaaaa agctatggtt ccatttctcg
    29641 gtgtgcatgt agcaagacac actcatgctt caatgctctt ggacgctgga gctactatgg
    29701 tagaagttca aactagatta cggcatacaa aaattagtca gacgatcgat tgttacggtc
    29761 acttggcaaa agaaacgaaa gaaaagactg ttgaaaaatt agtacaacac ttaaatattg
    29821 actagatggc atacaatatg gcatacagta aaaaataaaa aaaggagaaa ccctatcata
    29881 tcaaggtttc tcctagcttt atagtctatt cttcttcttc cgccatgaat gtattgaata
    29941 cttcttcaat catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc
    30001 catcttcgtt ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac
    30061 cagcaggata taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta
    30121 ggatttcata taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt
    30181 catggtcgtg ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa
    30241 ataattgctt acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat
    30301 cgatgacttt ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg
    30361 cctgaacagt ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga
    30421 tcttttctcc gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt
    30481 taggtaatcc aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat
    30541 caaagccaaa ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc
    30601 agccaaaggg gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta
    30661 ctctcataaa ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc
    30721 atcgcgttcg tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggacgg
    30781 gtctccagaa agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa
    30841 agcccgataa acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc
    30901 aaaacgtacg gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt
    30961 ccagcttacc ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt
    31021 ccaaacttcc gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc
    31081 tttatcaaaa gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta
    31141 cacaattttt tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt
    31201 gttgcttgtc ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca
    31261 ttgaattcaa tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc
    31321 agttcattcc cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg
    31381 atatatgccg caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc
    31441 cctgtcatca agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg
    31501 ctgcgattat ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca
    31561 atatacaaag gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct
    31621 tgtcctactc tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg
    31681 ctaagatcct cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt
    31741 ccatcatatt ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt
    31801 gtaatctgct gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca
    31861 atcacagcag cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc
    31921 ctttactaac agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga
    31981 aatagaaaca gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac
    32041 ggattcctcg tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa
    32101 attttcttca tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa
    32161 aagcttgcat ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct
    32221 tggctgttct tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac
    32281 gggaatcgaa cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg
    32341 caatacaaaa acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc
    32401 ttctggtatg ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg
    32461 acgggaaaag aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg
    32521 gaaagcaatg cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct
    32581 ggaaatttcc gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt
    32641 acctcgacca tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta
    32701 aaagtaaatg atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac
    32761 tgcgaactgc aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt
    32821 agcgcggcag tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta
    32881 cctgtgactt tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt
    32941 tttagaatca aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc
    33001 tacagcgtac gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg
    33061 cctttagttc ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat
    33121 gtctcaggag tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg
    33181 attgctttta ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt
    33241 attcagtttt cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct
    33301 aggcgttcat tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc
    33361 tgtgttcaaa taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca
    33421 ttcatcagcg atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc
    33481 gggatggttc ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact
    33541 gaatcctatc gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc
    33601 gctttaggtc ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag
    33661 cgtgacgcaa tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa
    33721 gaaaaggttg tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg
    33781 cgacaagtaa ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa
    33841 aatagcttct cacattttta cataaaattg acttaatact gagaaactga cttttgaaca
    33901 ccctctatct gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt
    33961 caaatttttt ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa
    34021 ctaaaccgat cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt
    34081 ataagacgat aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca
    34141 aacttcctga acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc
    34201 gataatctct agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata
    34261 gcaagctagg gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc
    34321 caccagttaa aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt
    34381 tcatagagat tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat
    34441 tgctgataaa agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa
    34501 caacaccgat taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag
    34561 caagaataaa aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag
    34621 gttcttttac taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt
    34681 tatgggttct ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg
    34741 gtactatcag ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc
    34801 aacccaataa accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata
    34861 tcgaaatcgg atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc
    34921 acatgattcc agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt
    34981 ttctatgttt tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac
    35041 aaaaaagaat aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa
    35101 tccctagatc agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag
    35161 gaatgatggt caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag
    35221 gcagaggcaa aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata
    35281 tactttgagt taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca
    35341 atttaacaaa gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg
    35401 aaaaaagatt ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc
    35461 ttgctttatt cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata
    35521 attgtcgctt atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa
    35581 atcttttcta gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa
    35641 atcaattcac ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat
    35701 acatgtgtag aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc
    35761 aaatcaaatg ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct
    35821 ggatctggca gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt
    35881 gcgattgttt cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc
    35941 ttttcttttt tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca
    36001 gccgtcaatt gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata
    36061 ctcaatcgat tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca
    36121 ataatactaa ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat
    36181 ccaaaaattt ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg
    36241 taacttttac ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact
    36301 ttttattgta cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag
    36361 gatagaaacg actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa
    36421 caggtaagat atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat
    36481 aaaattcttt agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa
    36541 tgttactatt tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc
    36601 ttttaaatct ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc
    36661 gtttacgaga atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat
    36721 gctcattaga taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac
    36781 agttgtccac ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa
    36841 gtaactttgg atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact
    36901 aggcgtaatc tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac
    36961 aattttacac ttttcattct tttctctaag catttcgcct agttcgatac cgctataata
    37021 ggcgttcaaa tcgatgtcta ggaaaaaaat atctgagg
//
LOCUS       DAZUKA010000008        34424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000008 DAZUKA010000000
VERSION     DAZUKA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.008"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8PN_000348"
     CDS             352..1041
                     /locus_tag="ACG8PN_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4860529.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8PN_000349"
     CDS             1028..2236
                     /locus_tag="ACG8PN_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4860530.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8PN_000350"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8PN_000350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4860531.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8PN_000351"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8PN_000351"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4860532.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8PN_000352"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8PN_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4860533.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8PN_000353"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8PN_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4860534.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8PN_000354"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8PN_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4860535.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8PN_000355"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8PN_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4860536.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8PN_000356"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8PN_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4860537.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8PN_000357"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8PN_000357"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4860538.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8PN_000358"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8PN_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4860539.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8PN_000359"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8PN_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4860540.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8PN_000360"
     CDS             14660..15043
                     /locus_tag="ACG8PN_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860541.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8PN_000361"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8PN_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4860542.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8PN_000362"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8PN_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4860543.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8PN_000363"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8PN_000363"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4860544.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8PN_000364"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8PN_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4860545.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8PN_000365"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8PN_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4860546.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8PN_000366"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8PN_000366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4860547.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8PN_000367"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8PN_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4860548.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8PN_000368"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8PN_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4860549.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8PN_000369"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8PN_000369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4860550.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8PN_000370"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8PN_000370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4860551.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8PN_000371"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8PN_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4860552.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8PN_000372"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8PN_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4860553.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8PN_000373"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8PN_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4860554.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG8PN_000374"
     CDS             28113..30086
                     /locus_tag="ACG8PN_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4860555.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8PN_000375"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8PN_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4860556.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8PN_000376"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8PN_000376"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4860557.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG8PN_000377"
     CDS             32965..34257
                     /locus_tag="ACG8PN_000377"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4860558.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10506 a         6694 c         6817 g        10407 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaac
//
LOCUS       DAZUKA010000009        33344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000009 DAZUKA010000000
VERSION     DAZUKA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.009"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1757
                     /gene="rny"
                     /locus_tag="ACG8PN_000378"
     CDS             201..1757
                     /gene="rny"
                     /locus_tag="ACG8PN_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4860559.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1836..2642)
                     /gene="proC"
                     /locus_tag="ACG8PN_000379"
     CDS             complement(1836..2642)
                     /gene="proC"
                     /locus_tag="ACG8PN_000379"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4860560.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2783..5356
                     /gene="mprF"
                     /locus_tag="ACG8PN_000380"
     CDS             2783..5356
                     /gene="mprF"
                     /locus_tag="ACG8PN_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4860561.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5510..6361
                     /gene="ispE"
                     /locus_tag="ACG8PN_000381"
     CDS             5510..6361
                     /gene="ispE"
                     /locus_tag="ACG8PN_000381"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4860562.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            6606..7544
                     /locus_tag="ACG8PN_000382"
     CDS             6606..7544
                     /locus_tag="ACG8PN_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4860563.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7567..8247
                     /locus_tag="ACG8PN_000383"
     CDS             7567..8247
                     /locus_tag="ACG8PN_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4860564.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8220..9044
                     /locus_tag="ACG8PN_000384"
     CDS             8220..9044
                     /locus_tag="ACG8PN_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4860565.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9211..10029
                     /gene="purR"
                     /locus_tag="ACG8PN_000385"
     CDS             9211..10029
                     /gene="purR"
                     /locus_tag="ACG8PN_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4860566.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10532..11905
                     /gene="glmU"
                     /locus_tag="ACG8PN_000386"
     CDS             10532..11905
                     /gene="glmU"
                     /locus_tag="ACG8PN_000386"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4860567.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            11999..12970
                     /locus_tag="ACG8PN_000387"
     CDS             11999..12970
                     /locus_tag="ACG8PN_000387"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4860568.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      12976..13078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13144..13713
                     /gene="thiT"
                     /locus_tag="ACG8PN_000388"
     CDS             13144..13713
                     /gene="thiT"
                     /locus_tag="ACG8PN_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4860569.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13794..14567)
                     /locus_tag="ACG8PN_000389"
     CDS             complement(13794..14567)
                     /locus_tag="ACG8PN_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860570.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14834..16534
                     /locus_tag="ACG8PN_000390"
     CDS             14834..16534
                     /locus_tag="ACG8PN_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4860571.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16504..16722
                     /locus_tag="ACG8PN_000391"
     CDS             16504..16722
                     /locus_tag="ACG8PN_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4860572.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16742..17632
                     /locus_tag="ACG8PN_000392"
     CDS             16742..17632
                     /locus_tag="ACG8PN_000392"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4860573.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17650..17934
                     /locus_tag="ACG8PN_000393"
     CDS             17650..17934
                     /locus_tag="ACG8PN_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860574.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18421..19035
                     /gene="gmk"
                     /locus_tag="ACG8PN_000394"
     CDS             18421..19035
                     /gene="gmk"
                     /locus_tag="ACG8PN_000394"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4860575.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19040..19345
                     /gene="rpoZ"
                     /locus_tag="ACG8PN_000395"
     CDS             19040..19345
                     /gene="rpoZ"
                     /locus_tag="ACG8PN_000395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4860576.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19598..22006
                     /gene="priA"
                     /locus_tag="ACG8PN_000396"
     CDS             19598..22006
                     /gene="priA"
                     /locus_tag="ACG8PN_000396"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4860577.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22021..22512
                     /gene="def"
                     /locus_tag="ACG8PN_000397"
     CDS             22021..22512
                     /gene="def"
                     /locus_tag="ACG8PN_000397"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4860578.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22505..23443
                     /gene="fmt"
                     /locus_tag="ACG8PN_000398"
     CDS             22505..23443
                     /gene="fmt"
                     /locus_tag="ACG8PN_000398"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4860579.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23443..24801
                     /gene="rsmB"
                     /locus_tag="ACG8PN_000399"
     CDS             23443..24801
                     /gene="rsmB"
                     /locus_tag="ACG8PN_000399"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4860580.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24814..25554
                     /locus_tag="ACG8PN_000400"
     CDS             24814..25554
                     /locus_tag="ACG8PN_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4860581.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25551..27620
                     /gene="pknB"
                     /locus_tag="ACG8PN_000401"
     CDS             25551..27620
                     /gene="pknB"
                     /locus_tag="ACG8PN_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4860582.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27785..28684
                     /gene="rsgA"
                     /locus_tag="ACG8PN_000402"
     CDS             27785..28684
                     /gene="rsgA"
                     /locus_tag="ACG8PN_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4860583.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28697..29347
                     /gene="rpe"
                     /locus_tag="ACG8PN_000403"
     CDS             28697..29347
                     /gene="rpe"
                     /locus_tag="ACG8PN_000403"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4860584.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29348..29986
                     /locus_tag="ACG8PN_000404"
     CDS             29348..29986
                     /locus_tag="ACG8PN_000404"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4860585.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30141..30995
                     /gene="mreC"
                     /locus_tag="ACG8PN_000405"
     CDS             30141..30995
                     /gene="mreC"
                     /locus_tag="ACG8PN_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4860586.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            30999..31508
                     /gene="mreD"
                     /locus_tag="ACG8PN_000406"
     CDS             30999..31508
                     /gene="mreD"
                     /locus_tag="ACG8PN_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4860587.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31763..33337
                     /locus_tag="ACG8PN_000407"
     CDS             31763..33337
                     /locus_tag="ACG8PN_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4860588.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        10988 a         5967 c         7218 g         9171 t
ORIGIN      
        1 aaccatcgtt taatgaataa gtagaaagcc gtttctttgt ttatttaaag ttgacactac
       61 ggggtacaaa cattagaata aagttgtgca tgttttttgt acacatatat gcgaagatgc
      121 atattgacga gtttagaata atttatcata gaagctgtat agatgataaa aatgaaaaag
      181 atagtatgga ggtggatttt atggtattca acattctcct cgctatcatc ggtttaattg
      241 tcggtcttgg tttagggttt atggtcgcaa agtcacgtca cgagaaagaa atagctggtg
      301 cacaatcatc agctgctgga atcatcgata gtgcaaaaaa agaagcagaa actctaaaaa
      361 aagaagcatt gttagaagct aaggaagaaa atcagaaata ccgttctgaa gttgaaagtg
      421 agctgagaga aagtaagtta gaattaaaat ctcaagagaa tcgtttgatt caacgcgaac
      481 aaacgctgaa tcgtaaagat gactcgttag aaaaacgcga gaactcactt gaaagtaaag
      541 aagaagcact tagttcaaaa cagcaattaa ttgaagagcg agaaaaagac gttgaaaaaa
      601 tgattgaaga tcaacaaaaa gaactagaaa aaattgctgg tttatcaaaa gaggacgcac
      661 gagatgtcat catgaaatcg actgaagaag agctgaatca tgagttaact ctaatggtaa
      721 aagaatctga acagcgtgcc aaagaagaag cagatcgcaa agcgaaaaac ttattatcat
      781 tagcgattca acgctgcgca gcagatcaag tttcagaaac gactgtttca gtcgttaccc
      841 ttccgaatga tgaaatgaaa ggtcgtatca ttggacgaga aggtcgtaat attcgaacgc
      901 ttgagacgct gactggtatc gatttaatca tcgatgatac accggaagca gttgttcttt
      961 ccggattcga tccaatccga agagaaatcg cacggatgac cttggaaaaa ctgatacaag
     1021 atggacgaat ccatccagcg cgtatcgaag aaatggtcga gaaatctcgt aaagaaatgg
     1081 atgaacgtat tcgcgaatac ggggaagagg cagcgtttga agtcggtgct catacacttc
     1141 atccagatct aatcaagatc atcggacgtc tgcgattcag aactagttat ggacaaaacg
     1201 tcttgaagca ttcggtcgag gttgcaaaat tagctggtat cttagcagca gagcttggcg
     1261 aagatatcca attagcaaaa cgtgccgggt tgctccatga tattgggaaa gcacttgacc
     1321 atgagattga aggatctcac gttgagatcg gtgcggaatt agctgccaag tacaaagaaa
     1381 acccagtagt aatcaacgcg atcgcttctc atcatggaga tgtggaagct acttctgtta
     1441 tctctgtcct tgtagctgca gcagatgcgc tctcagcagc aagacctggt gcgcgaagtg
     1501 aatcattaga aaactacatc cgtcgtttaa gaagtctgga aaatatttcg aacagcttcg
     1561 ccggtgtaga atcaagttac gcagttcaag ctggacgcga agttcgggta atggtcaagc
     1621 cagaagaaat ctcggattta gacgctgttc gtttagtacg tgatattcgt aaacgaatcg
     1681 aagaagaact ggattatcct ggacacatca aagtaatcgt tattcgggaa actcgtgcaa
     1741 ctgattatgc taaataataa aaaatacaaa agaagagccg cggcaaaagc cacagctctt
     1801 ctttttttgt aaggcacgaa aaaacattgc tatcattatt ttttattcaa catctcaaga
     1861 tctttctcga tcgtcgtatt gattcctttg atgattgctg ggatgaatcc tgcttcttct
     1921 aaggcaacta cacctgcaac agttgtaccg ccaggagaag aaacttggtc tacaagttcc
     1981 catggatttt tatcacttga aaggaatttt tgacaagaac ccataattgc ttttgcagca
     2041 atctcagtgg ctgtctgttt ggacaaacca ttcagtacac cagtccgtcc catcgcatcg
     2101 atcagcatgt aaacgaatgc gggtgagctg ccagcaaggc caatgaatgt actgaaatct
     2161 gattctgcta aaggatagac gctgccgatt gcttcaaaaa tcatcgtagc ttgctgaagc
     2221 tgttcctccg tggcagcttc atttttgcaa atcgcagaaa cgcctgctcc tacgctgaca
     2281 ttgacattcg gcatcacacg tacgatggag gcctgaggat cttcaagcgc tgtttctaaa
     2341 tctattaatg attttcctga agcaatagaa atgaataaga ctttctggct atattctctt
     2401 agttcttcca atactttttc aatgattttt ggtttcacgg ctaaaagaac gatatctgat
     2461 tgtgatgcaa cttcctgatt acttttgcat gcatggacgc cagtttcagc agcaaatttt
     2521 tgtgcatttt caaaatgtcc actggaaacg aacacctctt ctttcgagac tgttcctttt
     2581 tttaataaac catcaataat agcttgtgcc atattccctg tgccgataaa tcctactttc
     2641 atgttatcct ctcctttagc attaggatat ctttcttttt ctatcatcgc aagcaaagaa
     2701 caggtctaaa gtggaaaaat taaaataaat aagataacat tggggtatga cacaaaagta
     2761 tacaagttag gaatgggaaa atgtgaaaaa taaacgatac ttgatgctgc aatggataaa
     2821 ggaacatagt ctgattttaa aactgatttt tttcggctcc gtgttagtct ttgtggcaaa
     2881 tcaagtcgca aatattgcta atggcatgag ttggcaagat atttggcaaa ggatgggaca
     2941 acaaagccgc atgacgatcc tgctcatgat tctggctgga ctgctgggtg tcactccgat
     3001 gttgttgtat gattgggtga cgatcagagt cttagaaaaa caaggaaaac caaaaatgga
     3061 aagaaaagag tttcttcttg ctgcgtgggt aacaaacacg atcaacaatc tcgccggttt
     3121 tggtggtgtg gtaggagcaa gtctgcgggc tagtttttat ggaaagcaga caaatagaaa
     3181 aatggtttta gccactgtat caaaagtcgc actatttatg ctgactggtt tgtctgtatg
     3241 gtcgtttctt acatttattg atgtcttttt tattcagtca gaaagtattt ttcgaagcta
     3301 ttggatctgg cttttcggcg gtagtttttt agcaccagtc attttctttt ttgcttatct
     3361 aaagagaaag acattgttct tcgagtttta tccaaaaggc ttgcttggtc tgctactggc
     3421 atcctttgga caatggacgg gagcccttgc ggtatttttg atcatcggca agttgatgag
     3481 aatagatatt tcgatgcttt cgatctatcc tatgttcatg attgctacat tgataggaat
     3541 gctgacgatg gtcccaggtg gaatggggac ttttgacgtt ttgatgatct taggaatgag
     3601 tcaattgtct gttcggcaag atgtagcagt agtttggcta ttatattatc ggttgtttta
     3661 ttatgttcta ccttttatga cgggacttct attgttcatc acacatacag gagtcaaaat
     3721 caatcgattt ttagataaca ttccgcgact gtttttccaa aaggttgcgc atatcgtgct
     3781 cgtaagtgct gtctattttg cggggatcat gatggtactt ctatcaacaa tcactaatct
     3841 gtctgcaatc agtcggctat ttcaagtatt attgccgttc tcattcaatt ttttagatca
     3901 gacagtgaat ctattggttg gctttttatt actcggcctt gctagagcac tttcagtaaa
     3961 agtcaaaaag gcatttctgc ctacgattgc tttactatct tttggtattt taaatacaat
     4021 cacacgtacg ttatcttggc agttgatatt ggtatatgtc ttgattctgt tagctgtgtg
     4081 gttatccaga aaagaatttt atcgaacaaa attcgtttat tcttggggag ctataatctt
     4141 tgatgctagc ttgttcggct ttttgtttat cgtttacgca attgcaggct atcacagcgg
     4201 acgcttttta gtcggtcgaa taacaggaaa tcattttatt ttgtttcctt cagatgatgt
     4261 gtggttctca gggcttattg gtttgggcat ctcattgcta gctttagtgg cgctttatca
     4321 atatttagct gaagatacta cgaagcttgg cagtgagtac gaaaaagacc gttttgaccg
     4381 tttattggaa acatacggag gcacagctac aagccatcag cttcgcttgt caggttacag
     4441 ttatttttat taccaagaag acgaccaaga tcaagtcgtt tttggttatc agatcaaagg
     4501 aaacaaatgc tttgttttag gagatccagt tggcaatcct aaaatgatgc gggcagcaac
     4561 gaatcagttt atgaaacaag cagattgttt aggctatcag cttgcgtttt ataagatatc
     4621 agaaagctac gtagtgatgc ttcatgaatt gggttttcac ttcacgaaag taggtgaagc
     4681 agggattgtt gatctagcaa cagaagactt gtctcggctg agtcagcaac ttgaatatca
     4741 acgtttgatg aaagaaggat accattttac ttggtacgat caactgccgg aagaattaca
     4801 tcaagctgta aatgaagttt cacaaaaatg gcttcaaggt gcacgagaaa aatacttctc
     4861 tgtgggacga tttgataaat catatgtatt atctagcggt gtcggtattg tccgtaaaga
     4921 tcaaaaagtc gttggcttca tcaccgctca gccgatgacg aagaagcagg cgggatatga
     4981 tttgttgcgg gtcttaccta aagaatctga ggaattagct gactatctgt tagccaatct
     5041 gtttattgta tacgagaaaa tgggctactc ggaagccaat ctaggcttag ctccattagc
     5101 taatgttgga gaaacagatt tttctttctt tcaagaaaaa gtcatgcata tcgtatataa
     5161 ttatggtaat tttttctatt ccttccagtc tgcttatgaa aagaaattaa aatatgtcga
     5221 tcattgggaa ggacgttatt ttgcttatag gaaaggaagt aatttcggat ttgctaccat
     5281 gcagctactc agtctgatag gaaaagaaaa aggaaatgtt ccgagtttgg cagaagaagt
     5341 gctcacagaa ttataagaat cactagaact tgtttggatt atgaagagaa aattctgtga
     5401 aagctgttga aacgatgaca aaatcaacca ctaggcagac ataagctata cttttatttt
     5461 tccctatgat agaatacttg cgacttagac ttttcgaatg ggtgagagga tggaattgat
     5521 tgaaaaagcg ccagccaaga taaatttagg actggatgta ttatatagac ggcaagatgg
     5581 gtatcatgag ttggaaatgg tgatgtcgag cgtagacttg gcagatcatc tattttttga
     5641 agaaataaca gaagacaaaa tcattgtaga aacaaataaa gcatttttac cggtagatga
     5701 acggaacaat gtttatcaag ctgctttatt gctaaaaaat aaatacaata tcaaaaccgg
     5761 tgtcaagatc tttgttaaga agaatattcc agtagcagct ggattaggcg gaggaagtac
     5821 agactgtgca gctgctctaa gagggatcaa ccaattatgg aatctgggct gttcgttaga
     5881 agaactggca gaaatcggtt tggaagtcgg tacagatgtt ccttactgtg tctacggaag
     5941 tacggcattt gtcggtggca aaggggaaga aatccaagta ttaccttcta tgccacagtg
     6001 ctgggttgtg ttggtcaaac caagaatgag tgtctctaca cgtacagtgt ttagagaagt
     6061 agatacgcaa gccatccaac atcaagacat ccacggactt gctgaagcca tccgagagca
     6121 agattatcag cagatgatca aaaaaatggg aaacagttta gaagatataa cgggaaagcg
     6181 gcatccagtc atccatcaaa tcaaagagcg tatgatcaag tacggagcag atgctgcatt
     6241 gatgagtgga agcggaccaa ccgtttttgc cttgtgtcaa cattattcaa gagcgcagag
     6301 agtatataat gggttaaaag gtttttgtga ggaagtttat ttagtacgaa ccctgcaata
     6361 aaatataaga agtaaaatag gtgatgaaaa actgaggaat cctaaaatgg tattcttcag
     6421 ttttttattg tagtgaaatg gctagatatg agcagcaact catccatttt acgatttaat
     6481 caagaatata catgcttaaa tcagaaattg atggatttct attgactgtg tttttttatt
     6541 ttgatacatt agttcacgtt aataaatcgt aaaattccga tttgttcatt gaaaggagca
     6601 tataaatgaa aaaatatctt ttgacagcta cggtaatgat aggtgcttta ctgtttgcag
     6661 catgtggaaa tacgaacaag gaagcagaca aaaaagagga tttgacgatc gttacgactt
     6721 tttatccgat gtatgatttt acaaaagaga tcgtagggga tgaaggaaac gtgaaacttt
     6781 tgatccctgc tggaacggag ccgcatgatt tcgaaccaag tgcaaaagaa agagcagaaa
     6841 tttccgatgc agatgttttc gtgtataata gttcagacat ggaatttttt gtggattcgt
     6901 taaaggattc agtggatagc aaacagacct tgatgatcga agcagctaaa ggaatcgacc
     6961 gtttagaatc gcaagaagca gacgaacatg aagaatcaga agaagggcat ggtcattcac
     7021 atgaatacga tccacatgtt tggctcgatc cggtattagc tataaaagaa gtcagaacga
     7081 ttgctgaaga attaggcgaa aaatatccag ataaaaagga aacttttacg aagaatgccg
     7141 atgcttatat caaaaagctt gaagcactgg atcaaaaata ctccgatgag ctaaaagatg
     7201 caacaaaccg cacatttgtc actcagcatg ctgcttttgc ttatttagcg aatcaatacg
     7261 acttaaaaca agtagcgatt tcgggcgttt caccagatca ggaacccaca ccaagtcgct
     7321 tagctgagct aaaagaattt gtgaagaaaa acaacatcaa agtgatttat tttgaagaaa
     7381 atgcctcttc gaaggtagct gagacattgt cgaatgaaac aggagtgaag ctagaagtgt
     7441 tgaatccttt agaaagcttg actaatgaac aaatcaaagc aggcgaaaat tatatttctg
     7501 tcatggaaaa aaatctgaaa gctttaaaag aaagtatcaa ttaagtttta gaatggaggt
     7561 tgtaaggtgc gttatatcga aatatctgat ttgacctttt actatgatga tgaacctgta
     7621 ctagaagatg tctcttatca tgtagatgcg ggtgaatttg tcattttaac tggagaaaat
     7681 ggagcagcca agtcaacctt ggtgaaagca tcgttaggat tactgaaacc ttcaaaagga
     7741 atgataaaga ttgctgagaa aaatcaagaa ggaaaaaaat taagtatcgg ttatattcct
     7801 caacaagttg catctttcaa tgcaggtttt cctagtacgg tgatcgaatt agtccgttca
     7861 ggaagatatc caagaggccg ctggttcaag cgtctctctg aaaaagatca tcaacatgta
     7921 cagcgtgctt tagaagctgt agggatgtgg gatatgcgaa atcggcgaat aggtgaattg
     7981 tctggcggac aaaagcagcg aatcagttta gcgcgtattt ttgctactga ccctgactta
     8041 tttgttcttg atgaaccaac aacaggtatg gatgaagaat ccagaaatga attttatcgt
     8101 cttctccgac atagtgcgca tgtccatggg aaatcagttc taatgatcac ccatgatcat
     8161 gaagatatca aacagtatgc ggacagacag atccgactgg tgagaaagga ggattctcaa
     8221 tggcgttgct ttcatatgaa ttcatgagaa gagcgtttct tgccgctatc ttcattgccg
     8281 gaatcgcccc gatgctaggt gtgtttttag tgatcagaag acaatcgctg atggctgata
     8341 cgctttctca tgtctcttta gcaggggtag cgttaggatt ctttttgaac cttaatccta
     8401 cacttacgac catgctagtc gttgtattag cagctgtgat attggagtat ctgcgaacga
     8461 tctaccgaac ctattcagaa atctccattg ccattttaat ggctggtggt ttggcgctag
     8521 cgttagtatt gatgaatttg agcggaggga attcagcaac aagtatccaa tcctatctgt
     8581 ttggttcgat cgtgaccatt acatgggagc aagtcatctt tttaggaatc ttattcgttg
     8641 ccatcggtat attgttcttt ttattcaaac ggccgatgta tgttctgact tttgatgaag
     8701 ataccgccca cgtggatggg ctacccgttc atttgatgtc gatgatgttc aatgtgatta
     8761 ctggtgtggc tattgctgtg atgatcccga ttgccggtgc acttttgata tccgcaatca
     8821 tggttcttcc cgcagcaatc ggtatgcgat taggcaaaag cttcaatgtc gtgattgcta
     8881 tcagtgtttg tgtaggattt ctagggatgc tgtcaggact aactagctcg ttttatctag
     8941 atacacctcc aggagcaaca atcacattag tctttattgc tttgttcctg attgtaaata
     9001 tcatcaaacg aatattggtt gccttcagaa gaaaaaataa ctaaattata aagaaaagaa
     9061 gccttataaa aacgaaaaat tttttatagg cttcttttta ttttaataaa taccgaacta
     9121 ttttcttatt ttttcataaa atgctcgaat ttttgaagaa aaacttatat aatgtaaaac
     9181 gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt
     9241 gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc
     9301 gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca
     9361 tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg
     9421 ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt
     9481 ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga
     9541 gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa
     9601 atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat
     9661 tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca
     9721 gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag
     9781 agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga
     9841 acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact
     9901 gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt
     9961 tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt
    10021 tcagaataag aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa
    10081 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
    10141 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
    10201 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
    10261 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
    10321 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
    10381 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
    10441 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
    10501 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
    10561 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
    10621 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
    10681 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
    10741 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
    10801 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
    10861 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
    10921 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
    10981 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
    11041 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
    11101 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
    11161 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
    11221 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
    11281 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
    11341 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
    11401 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
    11461 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
    11521 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
    11581 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
    11641 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
    11701 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
    11761 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
    11821 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
    11881 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
    11941 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
    12001 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
    12061 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
    12121 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
    12181 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
    12241 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
    12301 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
    12361 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
    12421 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
    12481 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
    12541 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
    12601 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
    12661 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
    12721 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
    12781 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
    12841 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
    12901 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
    12961 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
    13021 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
    13081 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
    13141 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
    13201 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
    13261 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
    13321 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
    13381 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
    13441 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
    13501 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
    13561 tatgcactat gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg
    13621 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
    13681 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
    13741 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
    13801 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
    13861 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
    13921 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
    13981 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
    14041 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
    14101 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
    14161 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
    14221 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
    14281 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
    14341 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
    14401 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
    14461 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
    14521 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
    14581 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
    14641 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
    14701 ttttgtgatt tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa
    14761 tctacaaagt cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa
    14821 aaaggagagc gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc
    14881 gcctatccag caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga
    14941 cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac
    15001 cgtctatttt actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga
    15061 aggtgaaact tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt
    15121 tattaaagaa aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat
    15181 ttttcataaa aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac
    15241 agcacttgtt ttaggtagag gaggagcgca cggtgcttat cagattggtg tgtggcaggc
    15301 gctgaaggaa catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa
    15361 cggcgcattg attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc
    15421 gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt
    15481 ctatcgagaa agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc
    15541 tccattggaa aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa
    15601 aatacgttta tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat
    15661 ccaacagaca aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc
    15721 cgctatggct tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa
    15781 aatacctatt gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca
    15841 agggccaggc ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca
    15901 aacattgtgg acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt
    15961 acaattaggc tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac
    16021 atttcattct gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa
    16081 agaaattcag ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa
    16141 tttatataag aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc
    16201 aaaaaaacat tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat
    16261 ctgccaacaa gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag
    16321 tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa
    16381 aactgtttat tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt
    16441 gcttaagcaa ccagttgaca cattactgat cctatattta tattatctca aggaggaaca
    16501 accatggcac aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac
    16561 aacaaggggt ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc
    16621 gatcttcgag attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac
    16681 gaagagtttg aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg
    16741 aatgaaaagt atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga
    16801 tattgaaata aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat
    16861 cctgaacttt gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg
    16921 aatcgaagta tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa
    16981 ctgggaattg attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg
    17041 tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc ttcgtgctgg acgaaaactt
    17101 cgtaacggta gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga
    17161 tacattgaaa agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt
    17221 agctttagtg ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag
    17281 ttttgtagaa atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt
    17341 tcttggagcg aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg
    17401 cggagatatt cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact
    17461 tatcgaagca caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg
    17521 ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca
    17581 gtctaagacg atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag
    17641 gtagagataa tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt
    17701 aaacgggata tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac
    17761 tataatgaga acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg
    17821 aaatttattg ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct
    17881 cgaaaagatg agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga
    17941 tgctgaacaa agaaatacaa taaataaaca gaattggatt tcttcgctta gcttctttgc
    18001 tctaggaata catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa
    18061 taagtgatgt tcaaaaacca ttttctcggt attaagccaa atttatgcaa aaatgtgaga
    18121 aactattttc acaaaatttt tcttcatact cgaaactaat cggcttcttc acgacctctt
    18181 atacacggcg ttcaaaagct tgcatctgcg ataataaccc caaccaaacg aaaaacatga
    18241 gaagctgttt atgtttggct gttcttatta cttgatgcag gacagctttt ttacgacctc
    18301 ttggtacagg gtagcttttt taccaatttc tagctataat taaagataac cttgaaagtt
    18361 gcagaaaaaa agtgcaaaat aaacgataag tacaaaaaga ctagttgaaa ggacgtcgtc
    18421 atgtcagagc gtggattatt aatcgtgttg tctggtccat ccggtgtagg aaaaggcaca
    18481 gtacgaaaag caatttttga gagtgaggat aatgattttc agtattcgat ctcaatgact
    18541 acccgtaaaa tgcgtgaagg ggaggtcgaa ggcgttgatt atttcttccg aagcaaagaa
    18601 gagtttgaat caatgatcga agctggagag atgctggaat acgcagaata tgtcgggaat
    18661 tattatggaa ctcctttgtc atatgtcaac aagacattgg atgaaggaaa agatgttttc
    18721 ttggagattg aagtccaagg agcaaaacaa gtcaaagaaa aagtgcccga tggtgtattc
    18781 atctttttga ctccgccaga tttagcggaa ttaaaatcac ggatcgtagg ccgaggaaca
    18841 gatgcagatg aagtaatcga aaaacgaatg aaagttgcaa aagaagaaat cgaaatgatg
    18901 gctctttacg actatgctgt tgtaaatgat gaagtgccgc tagcagttga acgaatcaaa
    18961 aacattattg ccagtgaaca ttttcgagta gatcgcgtaa tcggtaaata tattaaaatg
    19021 ttaaaggaga tgtagtctta tgatgttaaa accttcaatt gactcactat tagatcgtgt
    19081 aaattcaaaa tattctctag tgattttagc aagtaaacgc gcgcatgaat tggatgcaag
    19141 tgcacagcct actctagatt cttttgattc agtaaaaagc gtcggtcaag ctttagaaga
    19201 aatcgatgca ggaaatgtag tcaatgatcc acatcctgaa ttaaaacgtg aacgtttgaa
    19261 gatggaagaa gaggaacgtc aagcacaaaa agagcgtgaa caacacgaat tggaatcacg
    19321 tatccgtgaa gagcaaaaaa tgtaataaag aaaaatagag cgaacatcta aaaagaaaag
    19381 caaacccagc tgtttccttt tcaaacattc gccacttatt gtctggtaga aaaggctgtg
    19441 tctgaacttt ttgacacagt tctttttttt cttatctgct gttaattttt tgttaggatt
    19501 tggcagaaga taagacaaat ccatcgatct ctagtaaaat aggcaaggaa ctttttgtcg
    19561 cttgataaga aaagacaaaa aaaggaggaa acgaaccatg acattaacag ctgaagtcat
    19621 tgtggacgta cctactatgc agaccgatca gccattcact tatttggttc cctctgaaat
    19681 ggaaacagct ttgcaagtcg ggatgcgagt agaagtccct tttggtaatg gcaatcgtca
    19741 tgtccaagga tttgttatgg ctatccaaaa atcagaagaa tcagctaatc catctttgaa
    19801 ggcagttatt cgtttgctag accttgctcc ggtggtcaat gaagaattac tttcattagc
    19861 tgattatatg aaaaaaatta cttacgcatt caaaatcact tgtctacaaa cgatgcttcc
    19921 gagtgtgatg aaagcagaat atgataaatt gatctatcct ttggcagaca caccagaagt
    19981 tcaggtattg tttgatcagc gagaagtgat ttcctggaag gaagcagaag aagaaggcag
    20041 cctttctcaa ttgattcgct ggcgccaaga gcaattagta gatatcaaat atgaagtcca
    20101 tacacgaaat aaagtaaaaa cgattcgttt ggtccgttct ttgttaactg aaaagcaaat
    20161 cgaagaagaa tgggcaaaat tacggcaaaa tgccaagaaa caaaaagaac ttcttttgtg
    20221 tttatctgaa ctgagccaag aagaaccaat tgcttatttc aaaaataaag gaatcagtac
    20281 tgctgtcttg aatcaaggaa aagaaaaggg ctggctggag tttatagaat cagaacgtta
    20341 tcgcgaccct tataaagacc gagtatttga ccaaacgacg gcattggaac tgaatgcaga
    20401 acaaaaaaat gctgtcgaac aaatcatcac agcaggtcag cagcaaaaag atcaggtctt
    20461 tttgctggag ggaatcactg gcagcgggaa aacagaagta tatcttcagg caatcgctga
    20521 tgttttgtca gaaaataaaa cggctattat gttagtgcct gaaatcgcat tgactccgca
    20581 aatggtcgag cgattcaaag gacgcttcgg agaatcagtt gcggtcttac atagcggact
    20641 ttcccaaggt gaaaaatatg atgaatggcg caagattgag cgggaagaag cgcaagtagt
    20701 agtaggcgca cgatctgcta tttttgcccc gctgaaaaat atcgggctga tcattatcga
    20761 tgaagaacat gaatcaagtt acaaacaaga tgagacaccg cgttatcatg cgagagatct
    20821 agcaatttgg cgcagtaaat atcatcattg cccaatagtt ctaggaagcg cgacgccctc
    20881 tttagaatca agagcacgag ctcaaaaagg cgtttatcag ttattgcagt taaaccaccg
    20941 agccaaagca gctgcacagc ttccagctat tgagatcgta gatatgcgtg aagagtttca
    21001 gaatcatcgg acctctactt tttctgctaa tttgcaggaa aaaatacaaa atcgattaga
    21061 caaaaaagaa caaactgtcc tactgctcaa tcgtcgagga tattcgtctt ttgtgatgtg
    21121 tagggactgt ggatttgttc ttccatgtcc taattgcgat atctctctta cactgcatat
    21181 ggatacacgt tcaatgcgct gccattattg cggacatgaa gaacctattc ccaatcgctg
    21241 tcctaattgt ggcggcaata aaatccgcta ctatggaaca ggtactcaaa aagtagaaga
    21301 ggaactccga gaactttatc cgcaagctag gattttgcgt atggatgtag ataccactcg
    21361 aagaaaaggg gcacacgaac agattttaca aaaattcggt gcaaaagaag cagatatatt
    21421 attagggaca caaatgattg ctaaaggttt ggactttcca gaagtgacat tagttggggt
    21481 gttgaatgct gatacctccc tgaatttacc ggattttcgt tctagtgagc atacattcca
    21541 attgcttacc caagtttcag gacgagcagg gcgagcggaa aaagcaggcg aagtcgtgat
    21601 ccaaacattc aatcctcaac attacgccat agaattggct aaaaaacaaa attatgagca
    21661 gttttatcag caggaaatgc atgtacggca tcgtggaggc tatcctcctt attactttac
    21721 tgtgaaaata accgccagcc atcctgaaga acaagtagcg gcaaagaaaa tctttcagat
    21781 tgccaatcag ctaaaagaag tgttaagccc tcaaagcttg ctattagggc caacgcccag
    21841 catgattttg agagtgaaaa accgatatta ttatcaacta atcatcaaat ataaacatga
    21901 accgaatctt cctcatgttt tggatgaaat tttgaatgga agccagaaag aacagcgaca
    21961 aggtcttttt gtggcaattg acaacgaacc attaaacttt atttaagaaa gggccaaatt
    22021 atgcgctatc ctatattgat ccaccctaat gataaattaa aaagaactgc tcagccaatc
    22081 gatgtaatca cggacgagac aatcgcttta ttagacaatt tatatgagac aatgattgcg
    22141 aatgacggaa tagggatcgc tgctcctcaa gtaggacaaa acaagagaat tgctgtgatc
    22201 gaagtagatg aaggcgagaa atttgaactg attaacccag aaatcatcga agcaaaaggt
    22261 gaaagccttg acgtagaagg ttgtctcagt attccgcatg tctatggaac ggtcaagcga
    22321 gcagatgaag tgaccgtgcg ttattatgat cgagacggag aagagataga agtgacggct
    22381 tttggctatt tagcccgtgc cttccagcat gaaatcgatc acttagacgg tatcttgttc
    22441 attgagaaaa tgatacagca gatcccggaa gaagaacttg aagaatatat ggaggaacat
    22501 cttgatgact aagctagtat ttatgggcac acctgctttt tctgtgccga ttcttgaagg
    22561 gctgatagaa tcaggttacg agatccaggc agtggtaaca cagcccgacc gtccagttgg
    22621 gcggaaacga gtaattacgc cgacaccggt aaaaaaagcg gctctgaagc acggtatccg
    22681 tgtgctgcag ccagagaaaa tctccggttc gccagaaatg gaagaaatca ttgaattggt
    22741 gcctgatgtg atcgtcactg cggcatttgg ccaattcttg cctgaaaagt tattgcaagt
    22801 accaaaatta ggggcaatca atgtccatgc ttctttactt cctaaatatc ggggaggtgc
    22861 gcctgttcat tatgccatca tgaatgggga gaaagaaact ggtgtaacga tcatggaaat
    22921 gatcaaaaaa atggatgcag gcggaatcta tgcgcaagaa agcataccaa tcactaaaca
    22981 agatgatgta gggacgatgt ttgaaaagct tagcctttta ggaagaaagc tattgctaga
    23041 aacattgccc aatattttgg atggacaaaa accagtacct caagacgagt cagaagcaac
    23101 tttttcgcca aacatcacac gggaacaaga agcaatcgat tggcataaaa ctgcagaaga
    23161 aattgacaac caagtacgcg gaatgcgtcc ttggccaatt gcattcacga cctatgaggg
    23221 catacgctgg aaattattat cggttgaagt attggcagaa actacagaca aagcacctgg
    23281 tacgatcatc cggaaagaca aaaaaaatct atggattgct tgcggtaaac agaccgtctt
    23341 agccatcaat gaattacagc cagctggtaa aggaaaacag gccgtacatg atttccttaa
    23401 cggaagcggt cagcaagttc agattggtca gcaggtgaaa taatgaaaaa aatccctaaa
    23461 aaaatgaaaa aatcagtacg atatatggca ttactgacat tagagcgagt gaacaagggt
    23521 ggcgcctact cgaatctttt gttgaatgag atgatcaaac aaagcgagct aaatcaaaaa
    23581 gatgtgggtt tatttacaga attgatctat ggtacgatta gccatcagcg actattggaa
    23641 ttttacgttg caccattgat cgctaaagca aaaaaagtgg atgactgggt caagatgctt
    23701 ctttatcttt ctgtatacca attagaattt ttagataaag tccctgcaca tgctatttta
    23761 aacgaggcgg tcgaaatcgc aaaagtaaaa ggaaatcctg ggacagggaa atttgtaaat
    23821 ggtgtcttga ggaattatca gcgtcaagga gcaccagatc tgcaaggcat tgctgatcct
    23881 atcgaaagac tttctgtcaa aatcagcctg cctctatgga tgacgaaacg actggtggat
    23941 caaatcggat acgaagaaac tgaaaagctc ggtttatcgc tatatcagcc tagccatgct
    24001 agtgcccgag tagatacacg ccgactttcc agagatcagg ccatcactgt tttgcaagaa
    24061 gaagagatca cagctgaaaa aagccaagtc agtccatacg gtatagtagc gaaaaaaggg
    24121 catttagctt ctagctcatt gttccacgat ggcgtcatga cgatccaaga tgaaagttcg
    24181 atgcttgtag cgccgtctat gcaaatcgag cctgaacatc atgtcttaga tgcctgtgca
    24241 gcacccggag gtaagacgac gcatattgct tcttttttag atccgtcatg cggtggagaa
    24301 gtggtcgctt tagatgtaca cgaacacaaa gtcaaactca ttgaagataa tgcagcacgt
    24361 ctaggtgtct cttctgctgt ctacgctcaa aagatggatg ctagagaagt cagtaaacag
    24421 ttcgaagcag agcagtttga tcggatttta gtagacgcgc cttgttcagg tcttgggctg
    24481 cttcggcgaa aaccagatat tcgttataaa aaacagccgg atgaattagc gaatttacca
    24541 aaaatccaat tggaaatttt agaaagctgc gcaccaactt taaagtcttc cggtattctg
    24601 acttatagca cttgtacgat cttaaaagaa gaaaaccaag acgttgtttc tgctttcttg
    24661 gaaagacacc ctgattttga gaggattgat gttttgacca atcctatgct acaatcatca
    24721 atcgaagaga aaatgttgac tttgtatccg catcagtttt acacagatgg atttttcatt
    24781 tgttgtttgc gcaaaaagta atgaggtgaa aaaatgcaga ttgaatatca atcagatgta
    24841 ggaagacgac gtaacacaaa tcaagactat gccagtgtat ttaccagccg ttcaggtctc
    24901 aagcttgctg tattagcaga cgggatgggt ggtcatcgtg ccggagacat tgccagtcag
    24961 atggcggtaa cgaatctagg cgaagcttgg gaaaaacaag aagctgttga cgatgaaaaa
    25021 atcgcccaat ggttcattca atcgattcaa gaagaaaata tgctgattta tcagcgtgga
    25081 caagaacagc cggaatataa cggtatggga acaacaatcg tagcggccgc tctgtcagat
    25141 gactggttca cgattgccaa tgttggggat agcagagctt atttgattcg tgaagataaa
    25201 atcatgcaat tgacggagga tcattcttta gtcaatgaac tagtcaaatc aggagaaatc
    25261 agcgaagaaa tggcagtgaa ccatccaagg aaaaatattt taacacgttc tgtcggcatg
    25321 cctggaacag tcgaagtaga tgtttctact catctttggc aactaaaaga tcgactgctg
    25381 ttatgttcag atggcttgac gaacatgctg tctgaagaag aaataggaac gatcatcaat
    25441 caagaaggcc cattgtctca taaagtaact gagctgattg atcgtgccaa tgaagcaggc
    25501 ggagcggata atatcaccgt tcttcttatc gaatataagg aggacagtgt atgatcgagt
    25561 tgggcaaaaa attaaatggc cgttatcaaa tcactggaaa tatcggtagc ggtggtatgg
    25621 caaatgtatt tttggcgcac gatttaatct tggatcgtga tgtagcagtt aaagtcttac
    25681 gatttgactt tcaaaatgac cagactgcga ttcgacgatt tcagagagaa gctttagcag
    25741 caacagaact cgttcatcca aacattgtta gtgtttacga tgttggtgaa gaagacaaca
    25801 tgcagtatct ggtgatggaa tacgtcaaag gaatggactt gaaacgatat atccaaacac
    25861 attatcctgt tccttatgaa acagcagtaa atattatgca gcagatcctt tctgctattt
    25921 ctttagctca taaccatcaa atcatccacc gagatttgaa accccaaaac gtactgatcg
    25981 ataatgaagg tgttgtaaaa atcacggatt tcgggattgc tatcgcttta tctgaaacat
    26041 cgatcacgca gacgaatacg atgctaggat ctgttcatta tctgtcccct gagcaggcca
    26101 gaggaagtat ggcaacgaag cagtcagata tttatgcttt agggatcatc ctttatgaga
    26161 tgttgacggg tagcgtaccg tttgatggtg aatctgctgt aacaattgct ttaaaacatt
    26221 ttcaagatga tcttccatct ataaaagcac ttgatccaaa tgtgccacaa gcactggaaa
    26281 acgtgatttt acgggcaacg gctaaagaac ctgcagaccg ttataagtca gccgaggaaa
    26341 tgtctgatga tctttctaca gtattatcac cagctcgtgc aaacgaagaa aaatggcagc
    26401 ctcatgtgat ggataatgaa acaaaagtta tcacacccct tacggaagat gcaccaatgc
    26461 ctgattcatt caaatctatg cctctgccaa aagacaagcc agaaaacgaa accttaccag
    26521 aggaggaaga agttctgcct gatcctaaaa agaaaggaca gaaaaaatgg tggctgatcc
    26581 tattagctat tcttgctgtt ttaggaatcg gaacagctgt attctttgct tctggcgggc
    26641 gaggagaagt tgttgttcct gatgtttctg atctttctga agcttctgct agagaaacac
    26701 tcgcaaaagc gaaattagaa gtagcaacaa aaacagaaga aattgcagat gacaaaatcg
    26761 aagaaggtaa tgtagtgaag acagacccgg cagcgggaac gacagtcaag caaaaacgcg
    26821 aagtcactct gtatattagt agcggcacga aaaaaatcaa attagatgat tacagtggta
    26881 aaagttatga agatgccgaa gcaagtttga aagaattagg gttttctgct gacttgattc
    26941 aaaaagaaga ggaattcagt tcagaggtag aaagcggaat gatcatttcg caatctccat
    27001 cagaagatac agaagtcgat cctaaaacag acacgattac tttcacagtc agcaaaggac
    27061 cagaaacagt gactttgagt agtcatgtag gagaaaacta cgaaactgtt cgtaattggc
    27121 ttgttacaca aggattcaat atcaataata ttagtgctac ttatgactat agcgacactg
    27181 taccatcagg ttcgatcatc agtcaggatc cagcctcggg tgaagttgta gcagaagaaa
    27241 cttacatcaa ttttgtcgta agccaaggaa cagagcctaa attgaatgat atctccggct
    27301 atacaaaaag cgaagcacaa agttatttag ctagtgtcgg tgcagaatat atcgggcatg
    27361 agacttccga atattcaaac acggtggata aagataaagt gattcgtaca gtaccagggg
    27421 caggaacgac aattaccaaa ggaatggttg tgaacgtaat ttactctaaa ggaccagatc
    27481 cagcgtcttc tagttctgaa aaagactctt cagacagttc tagcagctca agtagctcca
    27541 gtagcacaag caactcttca aaagaagaat cttccagctc cacaagttca tcagctagca
    27601 cctcagaaag ttcaaaataa gtgctgaata aaatatatca gaagggaatg cgacattgat
    27661 ttgttgcatt ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt
    27721 tgagaagttt gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac
    27781 ggaggtggca cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata
    27841 cgcaaacgga gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac
    27901 gccgcttgtc ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct
    27961 ggaaatcttg ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg
    28021 aatcattgtc acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct
    28081 agttaccttg gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc
    28141 agacgaccca gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt
    28201 gattgtaccc aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct
    28261 atcggtcttt atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc
    28321 ggaactgaat ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac
    28381 acgtcatgtg gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag
    28441 ctcgattgac tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt
    28501 agaagcttct gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga
    28561 agtaaaacat agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca
    28621 attcttacaa gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa
    28681 ataggaggaa acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt
    28741 acaaagagac attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat
    28801 ggatggccga tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc
    28861 tgttactaaa ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc
    28921 agcatttgca aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat
    28981 tcatcgggca cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc
    29041 aggaacacca gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat
    29101 gactgtcaat cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac
    29161 ggaactcgct gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg
    29221 aatcgttcca gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg
    29281 atcatatatc tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt
    29341 agactaaatg aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct
    29401 gctgtccaaa tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc
    29461 aggctaccca gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa
    29521 ggaagtattt gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga
    29581 tacacagtta ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat
    29641 tggagctacg ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc
    29701 tcgcttcaaa ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta
    29761 ttatttgcct ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg
    29821 ctgcttgact cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa
    29881 agaagtagaa tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt
    29941 ttctttttca gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata
    30001 aaaaaatttg tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta
    30061 agaggagcaa cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct
    30121 aattaattcg aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct
    30181 tggttattgt tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg
    30241 aaggaaagcc caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa
    30301 caatctctgc cccagtacgg tgggtacgaa atggagtaga ttctgtccat aatctttttt
    30361 caacttatgc cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac
    30421 agcaaaacaa gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc
    30481 agacgctgac tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt
    30541 ggcaggacat gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag
    30601 tcatgtcgca aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa
    30661 tcgaattact gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg
    30721 ctaatggcga agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg
    30781 tgacccaatt aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc
    30841 ttggagggaa ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata
    30901 gttatgggct ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag
    30961 ttgtgacgat tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga
    31021 agtactatct gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa
    31081 tgctagggga gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat
    31141 tattatgttg cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg
    31201 gggctatcta tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt
    31261 taattgtatg gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat
    31321 ttttcagtat gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga
    31381 tttttaaatt agcagtagta aataacacgt attttattac aaggttttta ggacctacac
    31441 tgctgttgaa catgattata tttgtattat tcatttttcc ctttaagaaa ttattcagtg
    31501 atgaataatt tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa
    31561 gaaatgctag acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca
    31621 tttttaaaag ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc
    31681 tatggtacaa tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt
    31741 aagccagtcg gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc
    31801 aatgaccttg actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca
    31861 gatacaacag caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc
    31921 acaaatcgat gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt
    31981 agcaaaacaa gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt
    32041 acaagaacgt attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt
    32101 aagcaacaca agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc
    32161 tattggacgc gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca
    32221 acaaaaacaa gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa
    32281 agaattagct gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca
    32341 agctgatttg aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa
    32401 gaaagctgac ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga
    32461 acaacaacgt ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga
    32521 agcacgcgaa caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag
    32581 ctcagcaaca gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc
    32641 tacacaatct tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac
    32701 agcgcctgaa tcttcagcaa cagaagaatc aacaacagcg cctgaatctt cagcaacaga
    32761 agaatcaaca acagtgcctg aatcttcagc aacagaagaa tcaacaacag tgcctgaatc
    32821 ttcaacaaca gaagaatcaa caacacctgc gcctacaaca ccatcaacag atcaaagtgt
    32881 tgatacagga aacggcacag gaagtagtac tccggctcca acgccaacac caacacctga
    32941 acaaccaaaa cctgtaacac ctgctccagc tccttctgga agtgtgaatg gtgcagcaat
    33001 cgtagcagaa gcttacaaat atattggtac tccatatgtt tggggcggta aagatccaag
    33061 tggctttgac tgctcagggt tcacacgcta tgtttacatg caagtaactg gtcgtgacat
    33121 cggtggttgg acagttcctc aagaatcagc tggaacaaaa atttcagttt ctcaagcaaa
    33181 agctggagac ttgttattct gggggtcaca aggcggaact taccacgtag cgattgcttt
    33241 aggcggagga caatatatcc atgctcctca accaggcgaa agcgtaaaag ttggttcagt
    33301 tcaatggttt gcccctgact ttgctgtcag catgtaagaa tgat
//
LOCUS       DAZUKA010000010        33324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000010 DAZUKA010000000
VERSION     DAZUKA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.010"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..404
                     /locus_tag="ACG8PN_000408"
     CDS             <1..404
                     /locus_tag="ACG8PN_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860589.1"
                     /translation="MMLSTALNNMIDSTECLFFLNTPNSISLSNEITNEQKFTYSPWL
                     YSELTTASIVEKKNPRLESNPQMSTEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKA
                     DLKKWALDAVECTKGSNTLDILYKNTIFTNL"
     gene            437..1114
                     /locus_tag="ACG8PN_000409"
     CDS             437..1114
                     /locus_tag="ACG8PN_000409"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFS4860590.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            1602..2567
                     /locus_tag="ACG8PN_000410"
     CDS             1602..2567
                     /locus_tag="ACG8PN_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS4860591.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            2589..2801
                     /locus_tag="ACG8PN_000411"
     CDS             2589..2801
                     /locus_tag="ACG8PN_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4860592.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            2876..3352
                     /locus_tag="ACG8PN_000412"
     CDS             2876..3352
                     /locus_tag="ACG8PN_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS4860593.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3349..5157
                     /locus_tag="ACG8PN_000413"
     CDS             3349..5157
                     /locus_tag="ACG8PN_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4860594.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5234..5449
                     /locus_tag="ACG8PN_000414"
     CDS             5234..5449
                     /locus_tag="ACG8PN_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS4860595.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5541..6404
                     /locus_tag="ACG8PN_000415"
     CDS             5541..6404
                     /locus_tag="ACG8PN_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS4860596.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6441..6977
                     /locus_tag="ACG8PN_000416"
     CDS             6441..6977
                     /locus_tag="ACG8PN_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4860597.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            6991..7413
                     /locus_tag="ACG8PN_000417"
     CDS             6991..7413
                     /locus_tag="ACG8PN_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS4860598.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7343..9742
                     /locus_tag="ACG8PN_000418"
     CDS             7343..9742
                     /locus_tag="ACG8PN_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS4860599.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            9774..11765
                     /locus_tag="ACG8PN_000419"
     CDS             9774..11765
                     /locus_tag="ACG8PN_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4860600.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            11788..12039
                     /locus_tag="ACG8PN_000420"
     CDS             11788..12039
                     /locus_tag="ACG8PN_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4860601.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12029..13258
                     /locus_tag="ACG8PN_000421"
     CDS             12029..13258
                     /locus_tag="ACG8PN_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4860602.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13255..15336
                     /locus_tag="ACG8PN_000422"
     CDS             13255..15336
                     /locus_tag="ACG8PN_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4860603.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15485..19405
                     /locus_tag="ACG8PN_000423"
     CDS             15485..19405
                     /locus_tag="ACG8PN_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4860604.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19406..20350
                     /locus_tag="ACG8PN_000424"
     CDS             19406..20350
                     /locus_tag="ACG8PN_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860605.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20354..20557
                     /locus_tag="ACG8PN_000425"
     CDS             20354..20557
                     /locus_tag="ACG8PN_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4860606.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(20857..21093)
                     /locus_tag="ACG8PN_000426"
     CDS             complement(20857..21093)
                     /locus_tag="ACG8PN_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860607.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21276..21647)
                     /locus_tag="ACG8PN_000427"
     CDS             complement(21276..21647)
                     /locus_tag="ACG8PN_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4860608.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21644..22972)
                     /locus_tag="ACG8PN_000428"
     CDS             complement(21644..22972)
                     /locus_tag="ACG8PN_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4860609.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22933..23262)
                     /gene="mobC"
                     /locus_tag="ACG8PN_000429"
     CDS             complement(22933..23262)
                     /gene="mobC"
                     /locus_tag="ACG8PN_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4860610.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23520..23890)
                     /locus_tag="ACG8PN_000430"
                     /pseudo
     CDS             complement(23520..23890)
                     /locus_tag="ACG8PN_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            24035..24220
                     /locus_tag="ACG8PN_000431"
     CDS             24035..24220
                     /locus_tag="ACG8PN_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4860611.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            24378..24545
                     /locus_tag="ACG8PN_000432"
     CDS             24378..24545
                     /locus_tag="ACG8PN_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860612.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            25163..25822
                     /gene="vanR-B"
                     /locus_tag="ACG8PN_000433"
     CDS             25163..25822
                     /gene="vanR-B"
                     /locus_tag="ACG8PN_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4860613.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            25825..27186
                     /gene="vanS-B"
                     /locus_tag="ACG8PN_000434"
     CDS             25825..27186
                     /gene="vanS-B"
                     /locus_tag="ACG8PN_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4860614.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(27277..28578)
                     /locus_tag="ACG8PN_000435"
     CDS             complement(27277..28578)
                     /locus_tag="ACG8PN_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4860615.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            28857..29663
                     /locus_tag="ACG8PN_000436"
     CDS             28857..29663
                     /locus_tag="ACG8PN_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4860616.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            29681..30388
                     /locus_tag="ACG8PN_000437"
     CDS             29681..30388
                     /locus_tag="ACG8PN_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFS4860617.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            30385..31356
                     /locus_tag="ACG8PN_000438"
     CDS             30385..31356
                     /locus_tag="ACG8PN_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4860618.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            31349..32377
                     /gene="vanB"
                     /locus_tag="ACG8PN_000439"
     CDS             31349..32377
                     /gene="vanB"
                     /locus_tag="ACG8PN_000439"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4860619.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            32383..32991
                     /locus_tag="ACG8PN_000440"
     CDS             32383..32991
                     /locus_tag="ACG8PN_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4860620.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(33057..33230)
                     /locus_tag="ACG8PN_000441"
     CDS             complement(33057..33230)
                     /locus_tag="ACG8PN_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860621.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         8600 a         8341 c         8883 g         7500 t
ORIGIN      
        1 atatgatgct ttctacagct ttaaataata tgattgatag tacagaatgt ctgttttttc
       61 ttaatacacc taattcaatt agtttgagca atgaaatcac taatgaacag aaatttacgt
      121 attcgccatg gctatatagc gaactgacga cagcttcaat agtggaaaaa aagaatccac
      181 gattggaaag taatcctcaa atgagcaccg aggatgttag aagcattata aagcactatt
      241 cttcgcaaga aaatctgcaa ataaaatatt ctttaaattt aagtgaatta acttatttac
      301 gaaaagctga tttgaaaaaa tgggctttag atgctgtgga atgcactaaa ggaagtaata
      361 cattagatat tttatataaa aacaccattt tcactaatct ctaatcaatt tactgaatcg
      421 aaaggaaccg ggtagaatgg ataagaaagt tttaataaag aggttctctg aagctattga
      481 aaaaggaaat gcagcaatat ttgcaggtgc aggattatcg atgtcacaag gatatgtaag
      541 ttggccggaa ctacttaatg atcctgcaac agaaatcggg ttggattcta aaaaagaaac
      601 agacttagtt actctggctc aatattataa aaatgaaaat ggtggatcaa gaggaatttt
      661 gaatcagata cttatggata attttggtga agaattagaa atatctgaaa atcatcgaat
      721 actagcaagt ttacctattg aaacttattg gacaactaat tatgatcatc taatagaaaa
      781 atcaatccga gaagcttata aaaaccctca ggttaagaaa aattatactc agttagcaac
      841 aactaatcca aatgtggata caattgttta taaaatgcat ggtgatatag atgatgtcag
      901 ttcgacagta ataacaagag atgattatga aaaatatgat gattcgtacg cgttatttaa
      961 agaaacttta aaaggagatt tgttaacaaa aacttttctc tttttaggat ttagttttac
     1021 agaccctaat ttagaaagaa ttttatcaga tataagatgg gttttgagag aaaaccaaag
     1081 acctcattat tgtataatga gaaaaatatt atagaaaaaa ttttaggtta tcgctgaaag
     1141 ccccggaaat acgggcattt cgcaggatat gagaactcaa tttactacca atttactact
     1201 ttcggattga gccgcatata tgctgaacca tactgctaat acccgccacc ccaaatacgg
     1261 cacatcataa attgaatacg acaccgtaaa cgcggcgttc ctctttcccg tcatgggaga
     1321 acaggacgcc gcttttttat gcccaaatag agaaaggagg cgacgccatg ctatttactt
     1381 ccggccctga ccgggcgttt gaacggctga tgcaggaaaa gcccggtttc gatcactacg
     1441 acggcggcgg ggcggacgcg ccggaggatt gcggcacctg ccgattttac cgcccggatt
     1501 ggaaatatca attctgccag tttgcagagt gtccctatca gccgggcaag ctgaccgact
     1561 atgcagcggt caaattcaca gtgaaaggag cgaacaacgc tatggcagtt ttccgtgtgg
     1621 aaaagaacaa aggctatacg gtgatgtcaa atcaccacct gcggaataag aacctgacct
     1681 taaaggccaa gggcctgctg tcgcagatgt tgtccttgcc ggaggattgg gactataccc
     1741 taaaaggact gtcctatatc aaccgggaaa gcatcgacgc gatcaggaca gcggtatggg
     1801 agcttgaaaa agctggatat atccggcgtg agcagggccg tgacgcaaaa ggcaaaatgg
     1861 ccgatatggt ctataccatc tacgaacagc cggtattgga aaacccggta ttggaaaatc
     1921 caacatcgga taacccggtg ttggaaaatc cgacatcgga taatccggcg tcggaaaatc
     1981 caacgcaatt aaataaagaa gtacaaagaa ctgacttatc aaaaaaagaa aaaataatta
     2041 cagatggaca aagtacccat tccattccta tcccatcccc gacccctgcc cctatggagg
     2101 gcgaggcagc ggctacgccg ccggaacgga aaaggacagg aaaggatgag gctgtacgga
     2161 tttatcggga aatcattttg gagaacatcg agtatgccta tctcattcag gacaggagca
     2221 ttgaccgtga acagcttgac gaaatcgtag acctgatgct ggaaaccgtc tgcaccgccc
     2281 gaaagacaat ccgtgtcgcc ggggacgact accctgccga gctggtgaaa tccaagtaca
     2341 tgaagctgac cggcgaacat atccgctttg tccttgactg tctgcgggag aacacaacga
     2401 aagtccgcaa tatcaaacaa tacctgcggg ctatgctgtt caacgccccc agcaccattt
     2461 ccaactacta cacggccctt gtcgcgcacg acatggcaca gcccgattgg ggaaagccta
     2521 aatccggcct gccggactat tcctgcggcg agggcgaaag cctgtaacga gaaaaggagg
     2581 acgaccctat gaagcagggc gcattgattt ttgacgagcg aacagaccgt tacgacatcc
     2641 gctttgatct ggccgactat tacggcggcc tgcattgcgg ggagtgtttc gacgtgatga
     2701 tcggcggcaa atggaggccc acccgcattg aaatggccga aaactggtat cttgtcggca
     2761 tccgggccga cgacctttcc ggcctgcggg tgcggatttg acctatatct acctgcggcg
     2821 tctggtggtt ccgccttaga cgccgctttt gactttctga caaggaggga tgcgattgca
     2881 ggaagaagtt actcaaaaga ccattgccct gtccatgaaa acgggcaagc tcacggctca
     2941 agtgctgcaa gcggcgctga aaaagtacct gcaacaccgg gccaagggca aaaacacgct
     3001 gcaccacggc cagcagagcc taaagcagct taaaaagcat ggggctgatc tctccaacat
     3061 tgagatcacc gaggccaaca ttggagcgtt caagccatgc gccaagaaat acggcgtgga
     3121 ttttacctta cgaaaagata agaccaccca gccgccccac tacgttgtga ttttcaaggc
     3181 caaggatgca gataaccttg atctggcgtt tcgggagttt accgccaaga cgctttccaa
     3241 ggagcagcgg ccctctatcc gcaaggtttt gtccgctgtg aagcaaaagg cggcggccca
     3301 gaccaagcag cgggccaagg agaaaatcaa ggaaaggggg ctggaactgt gaagcctgac
     3361 gtaaagaaat tgctgatcct gaacctcccg tatctgattt ttgtgtacct gtttgcgaaa
     3421 tgcggggagg cgtaccggct ggccgctggt gctgacctgt cggggaagct cctgcatttt
     3481 gcggatggtt ttgcggcggc ttttgccaac cctctgccaa gcctgcacct gtttgacctg
     3541 tgtatcggta tcgccggtgc cgtgatcgtg cggctggtgg tgtactgcaa gagcaagaac
     3601 gtgaagaaat accgcaaagg catggagtat ggcagcgccc gctgggggac ggccaaagac
     3661 atagcgcctt acatcgaccc caagtttgaa aataatatcc tgctcaccca aacggaacgg
     3721 cttaccatga ccggcaggcc caaagacccc aagacagcgc ggaataaaaa cgtgctggtg
     3781 atcggtggca gcggttcagg gaaaacgaga tttttcgtga aacccaacct gatgcaatgc
     3841 tttccgacca cggactatcc gacttcgttt gtggtgacag atccgaaagg cactttggtt
     3901 ctggaaaccg gcaggatgtt ccagcaggcg ggctaccgca ttaaaatcct gaataccatt
     3961 aacttttcca agtccatgaa gtacaacccc tttgtctaca tccattcgga aaaggacgtg
     4021 ctaaagctgg taaataccct gatttccaac accaagggcg agggggaaaa gtcagcggag
     4081 gatttttggg tgaagtcgga acggttgttt tattcggccc tgatcggcta tatctggtac
     4141 gaggccccag cggaggaaat gaacttctca acgctgctgg aaatgataaa cgccagtgaa
     4201 gcccgcgagg acgacccgga gtttcaaagc cccgtcgatc tcatgtttga gcgtttggag
     4261 cagaaagacc ccgaccattt tgcggtgcgc cagtacaaga aattcctgct gtccgcaggc
     4321 aagacccgtt cttccatcct gatttcatgc ggtgccagac ttgcgccctt tgacatccgg
     4381 gaggtacgcg agctgatgga ggacgacgaa atggagctgg acaccatcgg ggacgaaaag
     4441 acggtgctgt atctcattat gagcgatacg gacacgacct ttaatttcat tctggctatg
     4501 ctccaaagcc agctaatcaa cctgctttgt gaccgggctg atgatgtgta cggcgggcgg
     4561 ctgcctgtcc atgtacgcct gattttggat gaatttgcca acattggcca aatacccaat
     4621 ttcgataaac tcattgccac cattcgaagc cgtgaaatat cggcctctat tattttgcag
     4681 agccagtcgc agctaaaagc gatttacaag gacgccgccg agatcatttc ggataactgc
     4741 gatagtgtgc ttttccttag tgggcgcggc aaaaatgcca aggagatttc cgatgcgctg
     4801 gggaaagaaa cgattgacag tttcaacacc agcgagaacc ggggaagcca aacgtcccac
     4861 ggattgaact atcaaaaatt aggaaaggag ttgatgtccc aagacgaaat tgccgtgatg
     4921 gacggcggca agtgtatctt gcagctacgg ggtgtgcgcc cgtttttatc ggagaaattc
     4981 gatattacca agcatccccg gtacaaatac cttgccgacg ccgacaagaa gaataccttt
     5041 gacgtggaca gatttttgac tatcacgcgc cggaaacggc agcaagtggt aacgcaggat
     5101 gaagtattcg acctgtatga aatcgacctg tcggacgagg acgccgccgc tgaataaaca
     5161 gcggcttttt tcattccaac aacaaaatca aaaaatcatt atgagccgga aaccggcaga
     5221 aagaggtaaa tttatggcat tttttaattc cgcagttact actttgcaga ctatcgtgat
     5281 cggactgggc ggcgctctgt gcgtatgggg cggcatcaat ctgttggagg gatacgggca
     5341 ggacaatccg ggggcaaaat cgcagggtgt caagcagctt gtcgctggcg gcggcgtggc
     5401 cctgatcggt gtcactctgg ttcccctgct gtccgggctg ctgggctgat cctggccttt
     5461 gtccctgtcc aaacccttaa ctaatcgcct ccgcgtcctc ccggccagac cgggagggcg
     5521 cgggaaagga ggtgctaccc gtgattagtg aaatcattga agaatggata aaaggcatcc
     5581 tcatggacgg tatcacgggc aacctgtccg ggctgttcga tacggtaaat gccaaagtcg
     5641 gagaaatcgc cgccgacgtg ggacgaaccc cgcaggcatg gaacagcggt attttcaata
     5701 tgctgcacag cctgtcggaa accgtgatcg tccccatagc cggggccata ctcgcccttg
     5761 tcatgtgcta tgagcttatc cagatgataa cggaaaagaa caatatgcac gactttgaca
     5821 gctccatgtt cttccgctgg atattcaaat ccgcgtttgc aatcctgatc gtaacgaaca
     5881 catggaacat cgtcatgggc gtattcgacg ccacccaaag cgtcgtcaat caaagcgccg
     5941 gtgtgattat cgggcagaca tccattgatt ttaacaccct gataccagac cttgaaagcc
     6001 aattggaggc tatgagcgtg ggcgctcttt tggggctgtg gttccaaacc cttgttgtgg
     6061 gcctcaccat gaatatcctt tccatctgca ttttccttgt gacctatggc cgcatggtgg
     6121 aaatctatgt tgtcacgtcg ctggggccta tcccccttgc cacaatgggc aacgcggaat
     6181 ggcgcggcat gggccaaggg tatctgaaat ccctgctggc gctgggcttc caagcctttt
     6241 taatcatggt tgtggtgggt atctacgcgg tgctgataca gaatatcggt tccgcttccg
     6301 acatatccgg cgcaatctgg ggctgtatgg gctatacggt gctgctgtgc ttctgcctgt
     6361 tcaagaccgg cagtatatca aaggccgtat tcggggcgca ttgatcccaa aatccaagca
     6421 tacacaggag gaacttgttt atttcaacgt acaacatcat ttacgctgac ccgccgtggc
     6481 ggtatgactg caaaaacgta cagggcgcag cggaaaatca ctattccacc atgagcattg
     6541 acgagctgtg cgccctgccg gtggaacggc tggcgtccaa agactgcctg ctgtttttgt
     6601 gggccacatt ccccatgctg ccggaagctc tgcggttaat caaagcgtgg ggcttttcat
     6661 tcaagaccgt ggcttttgta tggctgaaac agaaccgtaa aagccttaca tggttttacg
     6721 ggctgggccg ctggacaagg ggaaacgcgg aaatctgcct gctggcaaca cacggcaagc
     6781 cgaagcgtcg ttctgcctct gtccatcagt ttattatcag cccgattgaa caacacagca
     6841 aaaagccgga tgtgacccgt gaaaaaatcg tagagctggc gggcgatttg ccccgcgtgg
     6901 agctgtttgc cagacagaaa acccccggct gggacgtgtg ggggaacgag gtggaatgtg
     6961 accttatcct gacgtgaaag gaggtgccaa atggcttatg taaccgtccc caaggactta
     7021 acccatgtga aaagcaaggt tgtgtttggg cttaccaaac ggcaactgat ctgtttttcc
     7081 ggggcgctgg tgatcggcgt ccccttatat tttctcaccc gtgactatct taccaacagc
     7141 gcggcggccc tgctcatggt atttgccatg ctgccgggct ttttgcttgc catgtacgag
     7201 cgtcacggcc agccccttga agtggtgatt atgcagatca tccagtgctg cttcatccag
     7261 ccaaaggagc gtccctatca gaccgacaac acctacaccg cccttgtgcg gcaataccag
     7321 atggaacagg aggtaaaggc cattgtccaa aaaggaaatc ccaagagaaa ccgaaaaaac
     7381 aaagctgacc cgcgcccagc gaaaagaaat tgacgcggta atccgtaaat ataagggcga
     7441 cggcaggccc cacacggcgc agcagagcat cccctacgag gtcatgtacc cggacggcgt
     7501 gtgccgggta tcgcccggcg tcttttccaa gtgcattgag tttgcggaca tcagctatca
     7561 gcttgcccag ccggacacgc agacggctat ctttgaaaaa ctgtgcgacc tgtataacta
     7621 tgtggacgcc tccattcata tccagttttc tttcctcaat cggaaagttg acccggtgca
     7681 gtacgccaag agctttgaga tcgcgccgca gggggacgac ttcgacgaca tccgggccga
     7741 gtacaccggc attctgcaaa agcagcttgc aaacggcaac aacggcatgg taaagaccaa
     7801 atacctgaca ttcaccatcg aggccgaaag cgtgaaagcg gcgcgagcca gattgaagcg
     7861 catcggcttt gacctgttag gctatttcaa gagcatgggg gccgtagccc atgtgatgga
     7921 cggctgggaa cggctgaacc tgctgcatgg cgtgtaccac ccggacggcg agatattcaa
     7981 ttttgactgg aagtggctgg ccccgtccgg cctctccacc aaggatttta ttgccccgtc
     8041 ctccctgtgc ttcggcaatg caaagacatt cggcatgggc ggcaaatacg gcgcggtaag
     8101 tttcctgcaa atcctgtccc cggagctgtc ggacgatatg ctggccgact tcctcaatac
     8161 ggaaagcggt gttttagtca atctccatgt gcaggccatt gaacagacca aggcgatcaa
     8221 gaccatcaag cgcaaaatta ccgaccttga cgctatgaag attgcggagc agaaaaaggc
     8281 cgtccgcagc ggttacgata tggacattct tcccagcgac cttgccacct acggcgagga
     8341 cgccaagaag ctgctgacaa agttgcagac ccgcaatgaa cggctgttcc agttgacgtt
     8401 ccttgtcctc aacgtggccg acaccaagca aaagctgaat aacgacgtgt tccaagccgc
     8461 aggcgtggcc caaaagcata actgtcccct tgtccggctg gactaccagc aggaacaggg
     8521 ccttgcaagc agcctgccgc tgggcgtcaa tcagatcaag atacagcgca gccttacgac
     8581 ttccagcgtg gccgtgttcg tcccctttgt gacgcaggag ctttttcagg gcggcgcggc
     8641 catgtattac ggcatcaacg ccaagtcccg caacatgatt atgctggaca ggaagcaggc
     8701 ccgttgcccc aatgccttga agctcggcac ccccggcagc ggcaaatcca tgagctgtaa
     8761 atccgagatc gtcagtgtgt tcttaacgac gcctgacgat atttttattt cagacccgga
     8821 ggccgaatat tacccccttg tcaaacggct ccacggacag gtaatccggc tgtcgcctac
     8881 cagcaaggac tttgtgaacc cgctggacat caacctgaat tattcggagg acgataaccc
     8941 gctggccctg aaatccgact ttgtgctgtc gttctgtgag cttgtcatgg gcggcaagaa
     9001 cgggctggaa gccattgaaa agaccgtgat agaccgggct gtgcgcgtga tctaccggcc
     9061 ctatctggca gacccccgcc cggagaatat gccgatcctg tccgacctcc acaaagcgtt
     9121 gttagatcag catgtcccgg aggctgaccg ggtggcgcag gcccttgacc tgtatgtgtc
     9181 cggctccctg aacgtattta accaccgtac caacgtggac atcggcaacc ggcttgtgtc
     9241 ctttgacatt aaggagctgg gcaagcagct caaaaagctg gggatgctca ttgtccaaga
     9301 ccagatatgg gggcgcgtca cggccaaccg aagccaagga aaggccacat ggtattttgc
     9361 cgacgagttc catttgcttt tgaaagagga acagacggcg gcctattctg cggagatttg
     9421 gaagcggttt aggaaatggg gcggcattcc aacaggtgcc acccagaacg tcaaagacct
     9481 gctttcctcc ccggaaattg aaaatatcct tgaaaacagc gacttcatta cgctgctcaa
     9541 tcaggcgtcc ggggatagaa aaatccttgc ggaacgcttg aatttgtcca ccgaacagca
     9601 gaagtacatc gacaattccg aacccggcga gggccttttg atctttgaaa acgtggtgct
     9661 gccctttacc aaccccatcc cgcacaacac ccagctttac aagattatga cgacccgctt
     9721 aaacgaggtg gcgggagtat gacccggcga cgccacaaag gaggtgaacc accatgaaag
     9781 agctgaaagc caaggcgaaa gtgacgcagg ccatgacccg cgacgggctg acgctggaaa
     9841 atcaggccac cggcgaggtg gagaacattt ccagccggga ggccgaacag gatttgtccg
     9901 cgccggggcc gggcggcacc gccgataagc tgctggaacg ggccgacgcc atccatgacg
     9961 gccataaggc caagaaagcc gcgaagcagg ccggggctgt ggccgccgag ggggacgctg
    10021 ccctgcaccg gccatccgcc cggctccaac tgtcggagga agaacgggcc gacccggatt
    10081 tacagcccta tatccgaaaa tcggacaaac gggccgataa gctggataag gcccgcgccg
    10141 ccatccccaa gaagcgcgtc ccggtcaagg aaaccgtctt tgacgaggca agcggcaaag
    10201 caaagaccgt cctgcacttt gacaaacagg acaaggggcc tccctccctg aaacccaatc
    10261 cggcaggccg tcctgtttcc gaggtttttc tattcggcca cgggaaaatc catgaagtcg
    10321 agcatgaaaa cgtgggtgtg gagggcggcc ataaggggga ggaacttgcc gagcggcagg
    10381 ccggaaaagc catccgcagc accatccggc accacaggct gaaaccctac tgggacgcgg
    10441 aaaaggccga gcgcaggcta ttgtctgcca acgcggagta tttctaccag aaatctttgc
    10501 gggataaccc ggagatcgca caggcggcca gcaaccccat ttcccggttc tggcagaaac
    10561 agcagatcaa gcgccagtac gccaaggccg cccggacagc gggccagacc gcaggcgcgg
    10621 cggcacaggg cgcggccaag acagccgggg cctccacgaa agcggccaag gccgcagcca
    10681 aagaaagtga acgggcggcc tcctttgtgg cccgccactg gaaaggggcg ctggccgtcc
    10741 ttgtgttggg gcttttgatg ctgcttatca tcggcggctt acagtcctgc accgccatgt
    10801 tcggcagcgc cggaagcggc atcacggcgt cctcttactt ttccgaggat agcgatatgt
    10861 tggccgccga ggacgcctac gcggagctgg aagccgagct gcaagactat ctggacaact
    10921 acgaggccac ccatgactat gacgaatacc attttgacct tgacgagatc agccatgacc
    10981 cctatgtgct gatttccatt ctgtcggcgc tccatgacgg gatatttacc atcggggagg
    11041 tacagggtga tttgtccatg ctctttgaca agcaatatat cctgacagaa aacgtcgtgg
    11101 tagaaacccg ttaccgcacc gaaaccgata catggacgga cgcggagggc aacacccata
    11161 cagacaccta ccaagtgccg tatgactatt acatctgcta cgtcacgctg gaaaacttcg
    11221 acctgtccca tgtgccagtc tatatcatgg acgaggaaac gctaagtttc tattccctgt
    11281 atatgtccac gctaggaaac cggcctgatc tgttttccgg cagccagtac cccaacgcct
    11341 ccaccctgac gccgccgacc tactacgacg tacccccgga ggctttggag gatgccacct
    11401 ttgccgccat gctcaaagag gcggaaaaat atatcggtta tccctatgtg tggggcggca
    11461 gctctcccag caccagcttt gattgcagcg gcttcatttc gtgggtaatc aatcatagtg
    11521 gatggaacgt gggacggctg ggggcgcagg gcctatataa catctgcacc cctgtttcca
    11581 gttcgcaggc caagcccggc gaccttgtat ttttcaaggg aacctacgac acccccggcg
    11641 tgtcccacgt cggcctgtat gtgggcaaca atgtgatgct gcattgcggg aacccgattt
    11701 cctacacgaa ccttacttca aactactggc aatcccattt ttacagttac gggcgtttac
    11761 cttaacgcca gaaaggagct ttcaattatg gcaaccaaac ttgaccgcat cgaaaaggac
    11821 atccagaaaa ccaagtctaa gatcgcggag tttcagaaac agcttcggga gctggaaacg
    11881 cagaaaaccg aacaggagaa tttgcagatc atccagcttg tgcgcggcat gaacatgaag
    11941 ccggaggaat ttgccgcgtt cttacgcagc ggcgcaatgc aggccgcccc tgccgcaacc
    12001 ccttaccatg aacaggagga caatgcccat gaagaataag accagacgga tttcccgtat
    12061 tctccccgca cttctggccg tgatgctctg cgtcacggcc tttcccgtta ccgcccttgc
    12121 aggcggcaat gacccggcac cggccccgct gccggaggct acaaccgaac ccgctacggg
    12181 cggtttggag cctgaaacag acgaaaccgg caccgccacg gacgggacag aaaccgaggc
    12241 cgaaaaagat accggccctc tgaccggcaa ggacatcacc gacctgtttt ccgcgctgtt
    12301 cggttccaag gtgagcattg ccgccacgga cgacggcatc cagatcacca ccggcaccga
    12361 caaaaaagaa cctgaacaga ccggcaccgt caccaccaac ggcggcaggc tgaatgtccg
    12421 caccggcgcg gggcttgaca agaccgcgtt cacccagctt cccaatggga cgacggttga
    12481 ggtagtcggc acagacggcg actggattaa aattctcttg ccggagcgca tcggctatgt
    12541 ccattccgac tatatgaccg tcagcgaaaa ggaagtagcg gctaccggcg agggcggttt
    12601 ttccctctcc attgacccgg aggaaattgc ttctctgctg gaactgttca atggcggcgg
    12661 ggccagcgcc gccctgtccc cggacgggaa cctgtcactg attgacgaca tcggcagcgc
    12721 cgcgaaaaat ggcaagcagt ttatcaccgt ggaaaccaaa aacggcaatg tgttttatct
    12781 gattattgac cgggacgacg agggaaaaga aactgtccat ttcttaaatc aggtggatga
    12841 agccgatctg ctgacgctga tggaggacgg ggaaaccgtc acccccgctg ctgtctgctc
    12901 ctgtaccacg aaatgcaagg ccggggctgt gaacacgaat tgcccggtat gtaaaaccaa
    12961 cctgaccgag tgcagcggcc cggagccgca ggaaccccag ccggaggaac ccgaagcccc
    13021 ggaggaagaa cccaagggcg gcgcgggcgg cctgatcgtg ttcctgctgg tggcgctggc
    13081 gggcggcggt gcggcccttt actatttcaa gttcaaaaag cccaaggctg aaaccaccgg
    13141 cagcgacgat ttgggcgagt atgattttgg ggaggacgag gatttggagg acgaggaacc
    13201 cgaaaccgag tttgacccgg aagctgattt tgtgagtgaa caggaggacg aggaatgaca
    13261 tataaacttg taatcgcgga aaaacccagc gtggcccgca gcattgcggg cgtgatcggg
    13321 gccacggaga aacacgacgg ctatttggag gggaacggct atctggtgag ctggtgcatc
    13381 ggacatctgg tgtcctttgc cgacgccgga cgctatgacg agcgtttcaa aaaatggcgc
    13441 tatgaggatt tgcccattct cccggaaaca tggcagtaca tcgtccccga cgacaagaaa
    13501 cagcagtttg acgttctctg ttcgctgatg cagcgccccg acgtgaccgg ccttgtgtgc
    13561 gccaccgacg ccgggcgcga gggggaattg attttccgtt tcgtctacca gatggcggga
    13621 tgccaaaagc cctttgagcg cctttggatt tcctctatgg aggacagtgc tattaaggac
    13681 ggctttgccc acctgaaacc gggcggcgac tatgatccgc tgtatcaatc ggccctgtgc
    13741 cgggccaagg ccgactggct gatcggcatt aacgccaccc ggcttttttc cgtcctgtac
    13801 cacaagaacc tgactgtggg ccgcgtccag acgccgacct taaaaatgct ggtggacagg
    13861 gacgccaaga ttaccgattt caagaaagag aaatatcaca tcgtccatat cacggcgggc
    13921 ggcgcggacg cggtaagcag ccgcttttcc gacgctgccg aggcaaacac cgtcaaggcg
    13981 gcttgtgcaa aagcgcaggc cgtttgcgtt tccgtgacgc gggagaaaaa gacggaacag
    14041 ccgcccaagc tctatgacct taccaccttg cagcgggagg ctaaccggct tttcggctat
    14101 accgccaagc agacccttga ctatgcccag gcactgtatc taaagaagct gctgacctat
    14161 ccgagaacgg atagtcaata tcttaccgac gatatgctgc ccgccgtgga aagccttgtg
    14221 tccggcctgt gggagctggt gccgtttgcc aagggcctga acctttcccc gcagtttgac
    14281 cgcatcctga acagcaaaaa ggtgtccgac caccacgcca ttatccccac cgctgaattt
    14341 gtgaagcagg gctttggcac tctggccgag agtgaaaaaa agctgctgtc cctgatctgc
    14401 tgcaagctct tatgtgccgt ggccgggccg catatctatg aggccgtcac cgccacattt
    14461 acctgtgccg ggcaggagtt taccgccaag ggcaagaccg tcctttcccc cggctggaaa
    14521 gagattgaac gccggttccg ttcttcctta aagaccgacg ccgacgagga cgccgaggcg
    14581 gtgcgggagc tgccggagct ttgcgagggc cagacctttg cagacgtggc cgcctctgtc
    14641 accgagcatt tcaccacgcc gcccaagccc tacaccgaag atacgctttt gtccgctatg
    14701 gaacgggccg gggccgagga tatgccggag gatgccgagc gcaagggatt aggcacaccg
    14761 gccacccgcg ccgccattct ggaaaagctg gtgcagatgg gctttgtcca gagaaaaggc
    14821 aagcagctca ttcccaccaa ggacggcatc aatctggctg tggtgctgcc ggaggcgctt
    14881 acttctcccc agcttactgc cgaatgggaa aaccggctga ccgagatcgc caaggggcag
    14941 gccgatcctg acgaatttat ggacggcatc gagacgcagg cgcgggagct ggtgcagacc
    15001 tattcctgta tcagtgagga aaagcagaaa ttgtttcagg ccgagcgtgt tcctatcggg
    15061 acgtgtcccc gctgtggtga ggctgtctat gagggcaaga aaaactacta ctgcggaaac
    15121 cgggcctgcc agttcgtcat gtggaagaat gaccgatttt ttgaggaacg caagaaagcg
    15181 ttcaccccga agatcgccgc tgcgctgctc aaaaccggca aagtcaaggt aaagggcctc
    15241 tattccgtaa agaccggcaa aacctacgac gggacggtgc ttttggctga taccgggggc
    15301 aagtacgtca actaccgcat tgaacacagg agctaaagga cagtaacaca gcaagcatag
    15361 ggagtgtggc tacactcccg gtaaaatccc ccggccgcgt ccctgccggg ccggggattt
    15421 ttgcttgttg gggaagtccc caacccccta ttttttgaaa cagaacagag aaaggagtga
    15481 ccgcgtgaac gaagtatttt ctatccaagt ccataaccgg cagtttttgg aacagggcaa
    15541 acaaggcgtc tggctggatt tgcccaccac aaccgaaacg ctgcaagccg ccctgcggga
    15601 tgttggcatt actgccgaca atccgcagga tttttttatc aacgggtatt cctgcccgga
    15661 ggacagacac cttgccattc cctatgacat ggtgctggcc gccgacgtgg acgagctgaa
    15721 tttccttgcc gcccggctgg gccagcttga cgccgccgag atcgcagagc tgaacgccgc
    15781 cctgcaaaat ccccgtggcg gctttgagaa tatcggccag atcatcgact acaccgaaaa
    15841 tgtggattac tatgtgcatc ttcccgacgt taggggggat ggacagttgg gcgactacta
    15901 cctgaaccgt tccggcatgg tggatatgcc ggaggaatgg aaagccggga ttgatacggc
    15961 gcagtttgga cgccacattg cccagcagga acaaggcgtt tttacccagt acggctatct
    16021 ggtgagaagc ggcgacgaat ggcagcgcgt ccatgagggc cagccggtgc cggaggaata
    16081 ccgggtgatg cactaccccc agccggaagc tgaccgggag gaaccgcagg cccagcagcc
    16141 ggaggcatcc gaaagccccg acaaagcgcc gcccccggtt atcccgatca tgctggacgg
    16201 catgaacagc aaagaacgca tgaaagtgat tactgaccgt ctggaaaccg gcgtacagga
    16261 gctttttgaa agtagccgct atatggctta cttatccgct atgtccaaga tgcacgatta
    16321 cagtctgaac aatacgctgc tgatcgccat gcagggcggt tcgattgtgg cgggctataa
    16381 caggtggcag gatgaatttg accgccatgt gaagcggggc gagaaaggca ttaagatact
    16441 ggccccggcc ccgttcaagg ttaagcagca gatggagaaa accgacccgg ccacccagca
    16501 gcccgtgatc ggcgcggacg gcaagcccgt gaccgaagaa caggaagtga cggttccggc
    16561 ctttaaggtg gtgacagtct ttgacatcag ccagaccgag ggcaaggatt ttcccatgcc
    16621 ctatatcagt gaacttaccg gcgacgtgca gcagtatcag gatttctttg ccgcgctgga
    16681 acggacatca ccctatccca taagcattga gccgatttca gacggggcca agggccgctg
    16741 cttctacgca aaacagcgca ttgccatcaa cgagggcatg agcgaattgc agaccctaaa
    16801 gaccgccatc catgagattg cccatgccaa gctccacgcc attgaccggg aggcccccgc
    16861 cgaggaacag gcggcccggc cagaccgcag cacccgcgag gtacaggccg aaagcgtggc
    16921 ctataccgtc tgccagtatt acgggctgga cacgtcggac tattcttttg gctatgtggc
    16981 cgattggagc agcggcaggg agttatccga gctgaaagcc tcccttgaaa ccatccgggc
    17041 cacggccagc gaattgataa ccgagatcga cggccatttt gcccagcttc agcaggagcg
    17101 cgaggcccgg caggaacagg aggccgtcac ggttgacgtt accccggagg cagcggcccc
    17161 ggcgcaggcc gaggaagaac aggccccggc ccttgatccg gcagccgagc cgcttgtgac
    17221 tgtgctatgg agcgaaagcc cccagctaaa ggacggccag caaatgccgt tgcatgaggc
    17281 cgacgcgctg tttgcccgtc tggatgcagc atatccggca gaggcaggct atgacaaaac
    17341 cgcgttccgc attgactgta tctttcaggg cgagccggac agctacgagg ggcggcagga
    17401 ttttggagat agggacggtt ccctgatcga acatatccag agctaccacc aattttacgc
    17461 gcaggacgaa aactggaaaa agcatgtaat cgctacaacc ggcatagagg gctgggagca
    17521 ggacaaggcc catcgggata tgctgttagg cgagtttgtc ccctatatga agctgcattg
    17581 cagccttgcc caaatggaac agacggcgca gggccgctta cagtccgggg aaaccctgac
    17641 gccggaggaa acggcctatt tgggggcgct tctctcttat gtgcaggagt gccgccccat
    17701 gctcaatcag ggccagtatc agttgccgga gccgccgaaa ctggctgact tcgaccagag
    17761 cttgcaggac tacaaagcac aggtacaggc cgagatcgcg caggaggccg ccgcagccgg
    17821 tatgaccgtg gaggaatacg cggcggctgg ctatgaagca ccccaacagg acaccttttc
    17881 catctaccag cttcgaggcg gggaggaaac ccgcgactat cggtttgagc catacgagcg
    17941 tttacaggcc gcagggctgg ccgtggacag tgccaactat gaccttgttt atacggcacc
    18001 gctggataaa agcacgtctt tggaggatat ttaccgcaca ttcaacatcg accacccggc
    18061 agattttagg ggacacagcc tttccgtttc cgacgtggtg gtgctgcacc agaatggaca
    18121 ggacaccgcc cactatgtag acagcttcgg ctatatggaa gtgccggagt ttttgcggga
    18181 aaatcccctg cgtaccgccg aactgtctac ggagcagaac gaaaacatga ttgacggcat
    18241 tatcaacaat acgccgcccg aaccgcagcc ggaggaaccc gaacagctat cttttgagga
    18301 tgtggcagtt gcttccggcc agacacagga ggaacccccg gaggctgcgc ccccgcaaac
    18361 ggctgtccgc tattatccca tcaatgaggg agccgcccgc cgcgccaagg aagccatgag
    18421 cttttctgac taccggcccg gcagcgccac ggcggaatac cggcactatg tcgatcaggc
    18481 cgtggaaatt gcggagcgcc agaaaaagcg tgtggagcca gagtatcacg aaaagataga
    18541 cagcctcctt gacacctacg cccgcaagct ggccgagaac atgaataagg gctatgagat
    18601 taccgcccgc gtcccctctg tcctgattgc cgggccgtcc aatttcccgg tacgggcgaa
    18661 agaaaagcag aacgccgcca gtgaccgcaa catggaggaa tttcagtaca ttcagggctt
    18721 gcttgacaaa atccgcagca ccggcatggg tggtatcagc gccgacgatc cgcaggccgt
    18781 ttccaagctg gaaaagaaac tggaaaagct ggaagcgtcg caggagttga tgaaggccgt
    18841 caatgcctac taccgcaagc atgggacgct ggacggatgc ccccatctga tcgaaagagg
    18901 cattgaaaac ctgaaagcgg atatggcgtc gggctggcac tatgagaaaa aaccgttcca
    18961 gtcctggcag ctctccaaca ataacgccga gatacgccgg ttaaagggcc gcattgagga
    19021 attgacccgc caaaaggagg cggcctatgt gggatgggag tttgacggcg gcactgtgga
    19081 gatcaaccgg gtggccaacc gcctgcaaat cttctttgag gggaagccgg atgctgctgt
    19141 acgcgacgag ctgaaaagca acggtttccg ctggtcgccc aaggccgggg catggcagcg
    19201 gcagttaaac gacaccacaa tccgcgtcgc tgaccgtatc aagtgcatcc agcccctttc
    19261 cggcgaaaag cccagcgcct tacagcttgc ggcccgcagg gagaaagaaa agccctctat
    19321 ccgggcgcag ctcaacgcgg ccaagaccga acaggccaag cagccaccgg cgcgggaaaa
    19381 atcaaaggga ttggaggtag agtaaatgca gaagtttgaa aatgtggacg ttctggccgc
    19441 gctggaacag atcatgcggc agaacaccgc gttttatcaa agtgactttg gcattgacaa
    19501 agacattatc aggcgggatg ccgccagcga tcaggccgca gacaagaccc tgctctggat
    19561 gtcccggccc tccgggacgt actgtttccg ggaacgtgat gttttcctga aagacacccg
    19621 acagtacaac acatggaaat tttacgggga gcagacccgt gacaagatac tggcctacgc
    19681 ggtggagctg accggcacac agggcggcac tatccggggc aatctctatg agctggacta
    19741 tccgcagcat ttccggcacg tcattgaaac cgcccagccg gtgagtgtga accggctctt
    19801 ttatgagcat ggcacccgtg acatcccgga gagccagcgt tttgacggaa gccctgaccg
    19861 ggtattggga aactttttgt actatgaggc ccagccccat gacccggctg tgctgcagga
    19921 ggcgctacgg acggagcagc acagccgggg gcgggccgtc cccggcgatt tcaaagtcca
    19981 cgtcaccgct ttgcatgaca gccggattga aaccgaggcc cggcgtattg tggacagcct
    20041 gaaagatctg tccgcgccta acagccccaa taaaacgcat ttcatggtgg agatctcccc
    20101 gcactttgcg gcgattgctt ccaccaagga taatgaccgg ctctttgcca tgctgcccta
    20161 taaatccctg tgttttaccg ggatgaaagg caggcatggc gtgtatgccg tgattaaccg
    20221 ggacgagcgc cgggatgtga gtattcgcag gccccggccc tctatccgca agcagctatc
    20281 cgacagcaaa caggccgcca gcccaaaaaa ggcggcggcc agaaccaaaa agaatgaatt
    20341 ggaggtataa ggaatgatgc actttaccgt ggaggaagaa aacctgatct gtatgtacca
    20401 caatgcagac cggcgcagaa cgataaacag gctcaccgcc gtcatgccgg ttatggatgg
    20461 ggatatgcgg gcgcaggccc agcagaccct ttccaagctg gaacgcatga cggatgccga
    20521 ctatgacggc cagaagttcc acttcacaga cgaataacga acagcagacg ccgggcgcgg
    20581 cggccatgtg gtcgcttgcg tccggcgtct tttctttttg cctattccta cgcatttaga
    20641 acccggtatt gtcggggata ggatgaagta ttcgcttgcc cctgcaaaca tgcgggagaa
    20701 gccttgcagc gggcggtttt caatccctta aatgcgtagg ttgcagctat aattagaccc
    20761 ctgaaacaga tatagccgcg cagcgggtgt atctgtttca ggggtgtgca ggtgatagcc
    20821 gcaaagcggc gtaagagggt gcagccctct tgccgtctat ctctgttcca gcgggaagct
    20881 cggcaatgct tccagcagtt tcagggaaat gcgctgccgt atatcttcgt caatgacttt
    20941 cttctttttg ccgtttgcct gtctgatctc caccgtggaa agctcgttga tgtagtcagc
    21001 ataatgggcc aacacttttt ccgtggccca cctctcaccg gcaacggcgg ctttgatcgt
    21061 ttcatagtcc agcagctttt cttgtccgtc catcctgata cccccgtcag actgattttt
    21121 tctatgatac catattcgga agcatggaac cagagaaaac ggcgcttttc tgctggtaaa
    21181 ttctacgcat ttagaacagc acttcggcgg ggataggata aaacacccac ataccccgct
    21241 gaccatgccg gaaaagcctt gaaatataag ggcttttaca gctctaagtg cgtaggtttc
    21301 cagtatcttt tccgctggcg ctcggcctct ttgcggcggc gcatccgttt ggagcaggcc
    21361 gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg ccccgcactc acagcagcgt
    21421 ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg ccgcatccag cgggaggaca
    21481 gcggccttaa accagcggca cagaagcgaa taggaaatgc tctgtacgca aatacattct
    21541 tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt tgcagcactc atgcgtcagc
    21601 ttccgggcct tgcgatattg agggtaattc attcgtggaa tactcatagc tcttgcgtcc
    21661 attcctttcc cggcgtcata cgcaaaatgc cgtccacatt ctgcttgatg atgccgtatt
    21721 cctgcatctg ctttttcgct tcgccgtact tctcatacag ccgttccttt tcctcggcca
    21781 atcgcttgta ctcggctttc agggcgtaca gatcggggag ctttttcaca ccctgtccct
    21841 ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct ttcatgctca cgccggaatt
    21901 ttgccttatc cgctgcattg cggtattcca tagccacggg ccagagctgc ttgtaggtag
    21961 aaatattctt tatcagcacc gccatatccg caagccggtg ttccatagct ttgagggtag
    22021 cggcggcctc gtcgctggcg gcggtgatct cggccacttt gctttccaaa tccgggtagg
    22081 tgtctatccc atgttcagtc aggaaattca aagtctttgc ggcctgcttc aaattgtgga
    22141 gcttcgccca cctctcatag cctgccgact gctgggcctt aatgttgttt tccagatcaa
    22201 tccgcaaaga aatccccttg cgctctttgg gcgctctggt gataacgcgg cctttgatac
    22261 gctctttgat ggcttcctcg gtgtaggctt cccccaatgt cttgcagcgg gtaaagcgtt
    22321 cctgtccggg agcgcggaaa gaaataaact ttcccggctt gatctcatag ccctgcgcct
    22381 gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg gatagcggcg tcaatggctg
    22441 ctttgagctt cgctttccag ctcgtccctt tccgctgggc gtcccactcg gcatagctct
    22501 tgcccttact ctggccgggc ttcaccacgg acaatccgtt ttccttgcac agcctgtcgc
    22561 tggtgcgccg gatataggca tagctctgtt tgttggaaat gtacttgcgc tgatttacca
    22621 tatccacggc acagaaaata atgtgattgt ggacgtggcc cttatcaata tgggtagtca
    22681 gcacataggg atatttccct tgcagcactt cctcggcaag ctgcttcccg atctcatggg
    22741 cctgttccgg cgtggcttcg ccgggggcaa aggattgtat caggtggtgg cccagattat
    22801 tgcccttttg gtatgcctgc gccagcagca tttcaaattc aatgtccgct gtttcataag
    22861 agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt gtccgggttc tcgatgtagt
    22921 caagggcttt tttcagcgtt gacttgatag gatgtatctt tgtaactgcc atatctgcgc
    22981 cagcgccccc tttatatcct cgatgtcctg tgcgtacacc ggccccatac cgttcagcct
    23041 ctttgcaatc tgattgacgt tgacgccgat cttctgtatc tcggcggtct gcgcccggat
    23101 gtcgctggtg tccatgttga cgatatagcc gtcaatgagc atcttccggg cgtaggctga
    23161 aaagttcttc gtatgcagca gcgccatttt ctgttcaatc atagcccgtt cctccggcgt
    23221 cacagggacg cgcagcacga tttttcgctt tctgtttgcc atgcttcacc gtcctttcca
    23281 ttcaggggtt tggggatttc cccaacaagc aaaacggacg cggcccggca gggacggggc
    23341 aggccgtttt tccgggagtg gctacactcc ctatgcttgc tacgttactg tttctccact
    23401 aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag ccctctggaa agaaaaaagt
    23461 gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa gtaaaacaga ggatgccagt
    23521 tacacatcct ccgtttcatt cttcttcggc ttttcaatta gtatggcgct gtcaatcgcg
    23581 ccctcgatga tcggcaggta ttcttcgggg cagagtttca gcttgtggct gaccctttgc
    23641 cgcagttcgc tttcctcccg catgatctcc gggttaaagt agcgttccac aggaagcccg
    23701 caggctttaa taagctgtat gacaaccggc agactgggaa tggcccgtcg ttttcgatgt
    23761 tggcaagata gcgccagtca atatttattt tctctgccat tgctttgcgc gaaacgcgct
    23821 ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc aaagccggga caatcttcaa
    23881 tattcgccat atcgcacctc ccacttacat tgtatatttc atactctgcc cgtggaatgt
    23941 tggtatatgc acctttctta aagtttatat ttcacatttt gtttctttta ttcttcgagc
    24001 aaaagaatta taatgtaccc tgtggaggtg tactatggac tacatgacgt tgaaagaggc
    24061 aggcgagaaa tggggtgtgc ccccacgatg gataaactac tattgtgctg cgggacgcat
    24121 ccccggcgct gtaaagatgg cgacgatctg gctcattccc aaaactgcgg aaaagccgat
    24181 tgacaggcgt acaaagaaag gaaaggcggt tactgaatag tataatgaaa ccggcgcatt
    24241 ttggatatgg ttttgaagag agagaaagca tggtattttg tcacggaaaa actataagat
    24301 gatagatggc aggctgctcc agaccgataa aaagtattct catctgaaac agaaagaaaa
    24361 gatttacaca tggatgtatg aagaagcgaa actttatcat gacagaaatg gaaaatgccc
    24421 gaaacaaaga cgaggaagct gctgctgtat cagccgtata tggtagaatt gaaaaggccg
    24481 gcatctggat tctttatggt gaagtctcca gacattggat aactgctggt ctaatgcgcc
    24541 tttgagtatg gggtgattga ttttaatacc ggggaggtac ggctttttga ggaaaatatt
    24601 ctgggattta catttggcga ttattacatt tcctgtaact gcaaggacac ggatgatgaa
    24661 gatatgagga atatagactt gctgattttc tactgtcttg aacggtatta ggactgaatc
    24721 tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca cacaggccct gcctgtgtga
    24781 aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc gcattttgct gaggcttggg
    24841 gtgtccccaa caagcaaaac gagaacgccg caggcgggca agtggtggat cagaatatcc
    24901 gtaaaggata ttctgatcca ttgcttgcca gtattatcgg gatgttttga caaccgctgt
    24961 aatcgaaaca tcccaaacat taagaaggtg ttaattatta ttatcttgat ttaattcgtt
    25021 taaaccaata atatagatac atacaccatg aaaagaagcg agtatcccaa tgacggatga
    25081 ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa ctacagactg ttatgttatc
    25141 ttcttaaaat ggagggattt ttatgtcgat acgaattcta cttgtcgagg atgatgatca
    25201 tatctgtaat acagtaaggg cgtttctggc tgaggcagga tatcaggtgg atgcctgcac
    25261 agatggaaat gaggcataca ccaagtttta cgaaaacact tatcaactgg ttattcttga
    25321 tattatgctg cccggtatga acgggcatga acttttgcgt gaatttcgtg cgaaaaatga
    25381 tactcccatt ctgatgatga cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga
    25441 tgcagaggca gacgactatg taacaaagcc attcaagatg cagattttac taaagcgggt
    25501 ggaagccctg ctgcggcgca gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac
    25561 acttctgccg gaggatttca cggtactttg tgacggtaca gagctgcccc tgacacgaaa
    25621 agaatttgaa atccttttgc tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat
    25681 cattttgtca cgtatatggg gatatgactt tgagggtgat ggcagcacag tccacactca
    25741 tatcaaaaat ttgcgggcca agctgccgga aaatatcatc aaaaccattc gcggtgtagg
    25801 ataccgattg gaggaatcat aataatggaa agaaaaggga ttttcattaa ggttttttcc
    25861 tatacgatca ttgtcctgct actgcttgtc ggtgtaacag caacactgtt tgcacagcaa
    25921 tttgtgtctt atttcagagt gatggaatta cagcaaacag taaaatccta tcagccattg
    25981 gtggaactga ttcagaatag cgataggctt gatattcaag aggtggcagg gctgtttcac
    26041 tacaataacc aatcctttga gttttatatt gaagataaag agggaagcgt actctatgcc
    26101 acaccaaatg ccaatacatc aaatagtttt agacccaact ttctttatgt ggtacataga
    26161 gatgataata tttcgattgt tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg
    26221 ctgacaattc ggggaattgt tatgattgcg ataatggttg tattcagcct tttatgcgcg
    26281 tatatctttg cgcggcaaat gacaacgccg atcaaagcct tagcggacag tgcgaataaa
    26341 atggcaaacc tgaaagatgt accgccgccg ctggagcgaa aggatgagct tggcgcactg
    26401 gctcatgata tgcattccat gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat
    26461 gaaatcgcaa gggaacatga gttggaggaa acacagcgat atttctttgc ggcagcttct
    26521 catgagctaa aaacgcccat cgcggctaca agcgttctgt tggagggaat gcttgaaaat
    26581 atcggtgact acaaagatca ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg
    26641 cagggcaaaa tcatttccga aatactggag cttgtcagcc tgaatgatgg gagaatcgta
    26701 cccatagctg aaccgttgga catagggcgc acggttgccg agttgctgcc cgattttcaa
    26761 accttggcag aggcaaacaa ccagcggttc gtcacagata ttccagccgg gcaaattgtc
    26821 ctgtccgatc cgaggctgct ccaaaaggca ctatccaatg tcatattgaa tgcagttcag
    26881 aacacgccgc agggaggcga ggtacggata tggagtgagc ctggtgctga aaaatgccgc
    26941 ctttttgttt tgaacatggg cgttcacatt gatgatactg cgcttccaag gctgttcacc
    27001 ccattctatc gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcag aaaaagtggg
    27061 cgaagcggtt taggacttgc catcgtacaa aaaacgctgg atgccatgag cctccagtat
    27121 gcgctggaaa acacctcgga tggcgttttg ttctggctgg atttaccgct cacatcaaca
    27181 ttgtaaatat ttaaaactta aatgattttg ggctctttgt caaataggac tgatagagtg
    27241 agataagaac atctaaggta gaattccttt tctactttag atgttttttg attcataggt
    27301 aatcagatta tttagtaaga aaattctggt cttcattttc cgaaagcttc gaaagccata
    27361 agcgactcgt ttcaatgctt tgatgtgggt atgcagattt tctattttgg catttgaata
    27421 aggcaattcg agggctaaac gaatgccgga ttcatagttt ataaaagctt tttttataga
    27481 atgcagttct tttggaacgg aattgggcat cgttcttaca agtttaaaga aagtgtccgc
    27541 ctctttgtcg tgaaacgctt gaagaatttc ttgatagtaa aaataggcga ttcgtaattc
    27601 gtcagagatc gaaagcagac gatccaccac ttcactttcg gtgagatacg cccaaccaaa
    27661 attgcgccat ttggtccatt tgtcataaac aagcttgtcc tgtcgtttga tcagaagccg
    27721 agacaacgcc tttaattgtc gataatattt ggcttgtgtt ggatcgtttc gatcaaagct
    27781 tttcatgatt cgtacgcgaa tatcctcaaa tttacgaatg agatgtttga caatgtgaaa
    27841 acgatccaca acgagttttg cctttggaaa acattccttt atcagagagg cataagaaac
    27901 attcatgtcc gtcacgatga cctttacctt caatcgtgcg cgtcgttcaa attgcataaa
    27961 ataagaaacg agagcctttt tcttccgtga aggaagaata tccagtaatt ttccagtatc
    28021 gccatccata caagaaaagg agtagggacc gacttgattt ttcagtgaac gaaattcatc
    28081 cattaataaa caacgcggta accaatttct ttttactttt gtcatgggga tcagagaagc
    28141 gagtgtccga gaaacggtcg accaagaaac gccgcaatgc ttagcaattg tcgtcattgc
    28201 ttgtttttca gttagctctt ctaaaatttt atagcgcact tgtttagcga taaagcagtt
    28261 tctatcgaca atgtaggtat tcgccgtaaa ataggtgtgg cagccacgac agtggtagcg
    28321 ttgtttcgct aaagcaagat aagtcagttg gttcgcacat ttattaagtt gaatgagcga
    28381 ttttttcttt ccgttcttaa cgatcatgcc ttcatgattc atctgtttac acaaaggaca
    28441 gcgacgaatt tctttactta acgtcgcaga aacaacaaga ctcttgatat tcttaatgct
    28501 cttcgattcc acatttgtga tgaaaatgtt tttatctttg attccaagta gtagtttgat
    28561 atgatgaaga tcaggcatac agaacgctcc tttgattgtg ttgtggtgat tcaattctaa
    28621 cggagcttct gtatgttttg ttttttcatg ctcaaaaggg accgatagaa tgattttcta
    28681 tcagtcctag aaattataga gccgattttg accgacaggt ataaccctgc cggtcttttt
    28741 ttgctgctac aggaaaacta cagactggct acagggaaag tacagatgcg cttgccataa
    28801 taacaatcgt accagccaca aatcgtagtt ttattgcaaa ggaggcattc gatcaaatgg
    28861 aaaaaagcaa ctatcattcc aatgcggatc atcacaaacg gcatatgaaa caatccgtgg
    28921 aaaagcgggc ttttctatgt gcgttcatta tttcgttcac agtctgcacg ctgtttttgg
    28981 ggtggagact ggcttccgta ttggaggcaa cacagatacc gcccatccct gcaactcata
    29041 caggcagcag cactgacgta gtggagaatt tggaggaaaa cgctcttgcc accgccaaag
    29101 aacagggaga tgaacaggaa tggagcctga ttttagtgaa caggcagaac cccatccccg
    29161 cacagtacga tgtggagctt gagcaactat caaatggtga gcggatagat attcggattt
    29221 ctccctatct tcaagatttg tttgatgccg caagaactga tggagtttac ccgattgtcg
    29281 catccggata ccgaacaaca gaaaaacagc aagaaattat ggatgaaaaa attgccgaat
    29341 ataaggcgaa aggctacacc tctgcacagg ctaaagcgga agcagaaact tgggtggccg
    29401 tgccgggaac gagcgagcat cagcttggtc ttgctgtgga tatcaatgcg gacggaattc
    29461 attcaacagg caacgaggtt tatagatggc tggatgaaaa cagctatcgt tttggtttta
    29521 ttcgtcgcta cccaccagac aagacagaga taaccggtgt gagcaacgag ccgtggcatt
    29581 accgatatgt tggcatcgaa gctgccacag agatgtacaa ccaaggggtt tgccttgagg
    29641 aatatttaaa accagaaaaa tgagaaaagg atgttatgtt atggacagaa aaagattgac
    29701 acagcgtttc ccgttcctgc ttccaatgag acgggcgcag agaaaaatgt gcttttatgc
    29761 gggaatgaga tttgacggct gtcgctatgc gcagacgata ggggaaaaat cgctttccca
    29821 tttgctcttt gaaacggatt gcgcattata caaccacaat accggatttg acatgatata
    29881 ccaggaaaac aaggtgttca acttaaagct ggcagcaaag accttaaacg gcttattgat
    29941 aagaccgggg gaaacctttt ctttctggtt gttcctgcat acgccattga caattatcca
    30001 gcgcagaggt cacgaagtaa aggagtttcc agagccaaac agtgacgaga ttaaaggggt
    30061 ggatgcaacc atttcagagg gctggattga tttaaaagtg cgaaacgata ccgactgcac
    30121 ctaccaaata tgggtgacct tagatgatga gaaaatcatc ggtcaggtgt tcgccgacaa
    30181 agagccccaa gcattataca aaattacaaa tggcagtatc cagtatgtcc gtgaaagtgg
    30241 cgggatttat gaatatgccc aggttaaacg gatgcaagtt gccttaggta ccggggaaat
    30301 aatagattgc aagctgcttt atacaaacaa atgcaaaatc tgttatcccc tcccggaaag
    30361 tgtggatatt caggaggaga accaatgaga aaaagtgtgg gcattactgt ttttggatgc
    30421 gaacaggatg aggcaaatgc tttccgcgct ttatcgccgg attttcatat tatccctacg
    30481 ctgattagcg atgcgatatc ggcagacaac gcaaaattgg ccgctggcaa tcaatgcgtt
    30541 agcgtaagcc ataagtccga ggtttccgag gcgacaattc ttgcgctgag aaaggtcggg
    30601 gtaaaataca tttctacccg cagcatcggc tgcgatcaca ttgatacgac tgccgccgag
    30661 agaatgggaa tctcggttgg cacggttgcg tattcgccgg acagcgttgc ggattatgct
    30721 ttgatgctga tgctgatggc catacggggt gcaaaaccca ccatgcacgc cgtggcgcaa
    30781 caagatttca gattggatcg tatccggggg aaagaactgg gggatatgac tgtgggagtt
    30841 attggaaccg gccatatcgg gcaagcggtc gtcaaaaggc tgcggggatt tggatgccat
    30901 gtgctggcct atgataacag ccgaaaaatg gatgcagatt atgtccagct tgatgagctt
    30961 ctaaaaaaca gcgatattgt tacgctccat gtgccgcttt gtgcggatac ccgccatctg
    31021 atcggtcaga agcaaattgg agagatgaag caaggcgcat ttttaatcaa caccgggcgc
    31081 ggagcgcttg tcgataccgg ggcgctggtg gaggcgttgg aaagcggaaa gctgggtggt
    31141 gcggcactgg atgtgttgga gggcgaggat cagtttgttt ataccgattg ctcgcagaaa
    31201 gtgcttgacc atcccttttt gtcgcagctt ttaaggatgc cgaatgtgat catcacaccc
    31261 catacggcgt actacactga gcgtgtgctg caggatacta cagaaaaaac aatcaggaat
    31321 tgtctcaatt ttgaaaggag tttacagcat gaatagaata aaagtcgcaa tcatcttcgg
    31381 cggttgctcg gaggaacatg atgtgtcggt aaaagccgca atagaaattg ctgcgaacat
    31441 tgatacggaa aaattcgatc cgcactacat cggaattaca aaaaacggtg tatggaagct
    31501 atgcaagaag ccatgtacgg aatgggaagc cgacagtctc cccgccatac tctccccgga
    31561 taggaaaacg catgggctgc ttgtcatgaa agaaagcgaa tacgaaacac ggcgtattga
    31621 tgtggctttc ccggttttgc atggcaaatg cggggaggat ggtgcgatac aggggctgtt
    31681 tgtattgtct ggtatcccct atgtgggctg tgatattcaa agctccgcag cttgcatgga
    31741 caaatcactg gcctacattc ttacaaaaaa tgcgggcatc gccgttcccg aatttcaaat
    31801 gattgataaa ggtgacaagc cggaggcggg tgcgcttacc taccctgtct ttgtgaagcc
    31861 ggcacggtca ggttcgtcct ttggcgtaac caaagtaaac ggtacggaag aacttaacgc
    31921 tgcgatagaa gcggcaggac aatatgatgg aaaaatctta attgagcaag cgatttcggg
    31981 ctgtgaggtc gggtgtgcgg tcatggggaa cgaggatgat ttgattgtcg gcgaagtgga
    32041 tcaaatccgg ctgagccacg gtatcttccg catccatcag gaaaacgagc cggaaaaagg
    32101 ctcagaaaat gcgatgatta cagttcccgc agacattccg gtcgaggaac gaaatcgggt
    32161 gcaggaaacg gcaaagaaag tatatcgggt gcttggatgc agagggcttg cccgtgttga
    32221 tctttttttg caggaggatg gcggcatcgt tctaaatgag gtcaataccc tgcccggttt
    32281 tacatcgtac agccgctacc cacgtatgat ggccgccgca ggaatcacgc ttcctgcact
    32341 gattgacagc ctgattacat tggcgttaaa gaggtgataa gtatggaaaa tggttttttg
    32401 tttttagatg aaatgttgca tggcgtccgt tgggatgcca agtacgccac atgggataac
    32461 ttcacaggaa aaccggtgga tgggtatgag gtgaatcgca tcatcggcac aaaggccgtg
    32521 gcgtttgctc tgcgcgaggc acaaatccat gcggctgcgc ttggctatgg cttgctttta
    32581 tgggatggat atcggccaag aactgcggtg gactgcttcc tgcgttgggc agcgcaaccg
    32641 gaggacaatc tcacaaaaga aaaattttac cccaatatag agcgagccga gttgattaca
    32701 aagggttatg tggcctcaca atccagccat agccgtggaa gcgcaattga tcttacgctc
    32761 taccacctgg atacagggga acttgtttca atgggaagta acttcgattt tatggacgaa
    32821 cggtcgcatc atacagcaaa agggataggg gatgcagagg cacaaaatcg aagatgcttg
    32881 cgtaaaatca tggaaagcag cggatttcag tcttatcgct ttgaatggtg gcactataag
    32941 ttgattgatg agccataccc cgatacctat tttaattttg ccgtttcata atgaaagtat
    33001 tggttttgta tcagattttg gatttgataa aatctatgct ggtcatggac aatcttttac
    33061 tggaaagtgg ttaaagcatc ttttgtaata cattgcaaat tatagtattc aatctgccca
    33121 gcggcagaaa agaaaaaaac cgctgttggg cagacctttc ccacgcccat tttgtatttt
    33181 gctggcggcg gttttgagaa atcaaggccg ccgttctttt ctggcagcaa aaggtgtggc
    33241 ggcccatagg aggatgccgc tatgctgttt tgctattgcc atgctctaat ccacttccac
    33301 cactcaaaaa aagccttgcc cctc
//
LOCUS       DAZUKA010000011        33005 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000011 DAZUKA010000000
VERSION     DAZUKA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.011"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..820
                     /locus_tag="ACG8PN_000442"
     CDS             <1..820
                     /locus_tag="ACG8PN_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4860622.1"
                     /translation="AILLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTENPGKF
                     INVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIVNKDI
                     GVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGETAVQ
                     AQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWTIWAY
                     EHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE"
     gene            935..1855
                     /gene="pstC"
                     /locus_tag="ACG8PN_000443"
     CDS             935..1855
                     /gene="pstC"
                     /locus_tag="ACG8PN_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4860623.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1855..2739
                     /gene="pstA"
                     /locus_tag="ACG8PN_000444"
     CDS             1855..2739
                     /gene="pstA"
                     /locus_tag="ACG8PN_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4860624.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2754..3560
                     /gene="pstB"
                     /locus_tag="ACG8PN_000445"
     CDS             2754..3560
                     /gene="pstB"
                     /locus_tag="ACG8PN_000445"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4860625.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3576..4334
                     /gene="pstB"
                     /locus_tag="ACG8PN_000446"
     CDS             3576..4334
                     /gene="pstB"
                     /locus_tag="ACG8PN_000446"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4860626.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4347..5024
                     /gene="phoU"
                     /locus_tag="ACG8PN_000447"
     CDS             4347..5024
                     /gene="phoU"
                     /locus_tag="ACG8PN_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4860627.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5601..6293
                     /locus_tag="ACG8PN_000448"
     CDS             5601..6293
                     /locus_tag="ACG8PN_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4860628.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6290..7057
                     /locus_tag="ACG8PN_000449"
     CDS             6290..7057
                     /locus_tag="ACG8PN_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4860629.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7157..8725
                     /gene="liaX"
                     /locus_tag="ACG8PN_000450"
     CDS             7157..8725
                     /gene="liaX"
                     /locus_tag="ACG8PN_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4860630.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9022..9342
                     /locus_tag="ACG8PN_000451"
     CDS             9022..9342
                     /locus_tag="ACG8PN_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4860631.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9342..9692
                     /locus_tag="ACG8PN_000452"
     CDS             9342..9692
                     /locus_tag="ACG8PN_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4860632.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9859..10797
                     /gene="hprK"
                     /locus_tag="ACG8PN_000453"
     CDS             9859..10797
                     /gene="hprK"
                     /locus_tag="ACG8PN_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4860633.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10812..11642
                     /gene="lgt"
                     /locus_tag="ACG8PN_000454"
     CDS             10812..11642
                     /gene="lgt"
                     /locus_tag="ACG8PN_000454"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4860634.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11686..12711
                     /locus_tag="ACG8PN_000455"
     CDS             11686..12711
                     /locus_tag="ACG8PN_000455"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4860635.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12726..13664
                     /gene="galU"
                     /locus_tag="ACG8PN_000456"
     CDS             12726..13664
                     /gene="galU"
                     /locus_tag="ACG8PN_000456"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4860636.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13789..14229
                     /locus_tag="ACG8PN_000457"
     CDS             13789..14229
                     /locus_tag="ACG8PN_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4860637.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14232..14834
                     /locus_tag="ACG8PN_000458"
     CDS             14232..14834
                     /locus_tag="ACG8PN_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4860638.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14893..15999)
                     /locus_tag="ACG8PN_000459"
     CDS             complement(14893..15999)
                     /locus_tag="ACG8PN_000459"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4860639.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16209..17210
                     /gene="ccpA"
                     /locus_tag="ACG8PN_000460"
     CDS             16209..17210
                     /gene="ccpA"
                     /locus_tag="ACG8PN_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4860640.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17547..19892)
                     /locus_tag="ACG8PN_000461"
     CDS             complement(17547..19892)
                     /locus_tag="ACG8PN_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4860641.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20171..21073
                     /locus_tag="ACG8PN_000462"
     CDS             20171..21073
                     /locus_tag="ACG8PN_000462"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4860642.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21286..23061
                     /locus_tag="ACG8PN_000463"
     CDS             21286..23061
                     /locus_tag="ACG8PN_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4860643.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23127..24713)
                     /locus_tag="ACG8PN_000464"
     CDS             complement(23127..24713)
                     /locus_tag="ACG8PN_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4860644.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            24964..25188
                     /locus_tag="ACG8PN_000465"
     CDS             24964..25188
                     /locus_tag="ACG8PN_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4860645.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25329..27080
                     /locus_tag="ACG8PN_000466"
     CDS             25329..27080
                     /locus_tag="ACG8PN_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4860646.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27080..28864
                     /locus_tag="ACG8PN_000467"
     CDS             27080..28864
                     /locus_tag="ACG8PN_000467"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860647.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29190..30041
                     /gene="licT"
                     /locus_tag="ACG8PN_000468"
     CDS             29190..30041
                     /gene="licT"
                     /locus_tag="ACG8PN_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4860648.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30137..32017
                     /locus_tag="ACG8PN_000469"
     CDS             30137..32017
                     /locus_tag="ACG8PN_000469"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4860649.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32057..32959)
                     /locus_tag="ACG8PN_000470"
     CDS             complement(32057..32959)
                     /locus_tag="ACG8PN_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4860650.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10468 a         5868 c         7012 g         9657 t
ORIGIN      
        1 agctattctt ttatcaggct gttcaaagtg gattgatcga ggagaatcca ttacggctgt
       61 tggctcttct gctttacagc ctttagtaga aacagttgct gaaacttacc agacagaaaa
      121 tcctggaaaa ttcatcaatg tacaaggggg cggaagcgga actggtttgt ctcaagtaca
      181 gtcaggagca gtcgacatcg gaaactctga tttgtttgcg gaagaaaaat cggggatcga
      241 tgcctcagca ttagtcgatc atcgagtagc agttgttggg atcactccta ttgtgaacaa
      301 ggatattggc gtaaaagaca tcagtatgga aaacctgaaa aaactattta caggcgagat
      361 caaaaactgg agtgaagtcg gtggaaaaga tcagccagta gttatattga accgcgcttc
      421 aggaagcgga acccgtagca cgtttgaaaa atgggtattg gacggagaaa cagctgttca
      481 ggcacaagaa caagattcaa gcggaatggt gcgccagatc gttgccgaca cacccggtgc
      541 aatcagctat gttgcttttt cttatgtttc cgatgatgta gatacgttga atgttaacgg
      601 tgtagaaccg acagatgaaa atgtgacaac gaacaaatgg acgatttggg catatgagca
      661 tatgtacaca agagatcatc caaaaaaact gacaaaagaa tttttagact atatcttatc
      721 tgatgaagta caagataaca tcgttggaga actaggttat atccctgttt ctcatatgaa
      781 agtcgaacgt gattggcaag gaaatatcgt taaagagtaa tctttacaca aaatttacaa
      841 acttagctaa acttttacat gttattaaaa ccaaattgat gttcaatttg tacactgagt
      901 gagtatgtta aacctgatta agggggagtc atctgtggaa gatgtgaaaa aagtattatt
      961 aacaaaatca aaacgttcaa aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat
     1021 cgcgctgatc gtgttggttg tattatctat tttttatttt gtagctagta aaggattggc
     1081 cacctttttt actgataaga tcaatgtatt tgattttctg tttggtacag attggaatcc
     1141 aagccaaatc ggagcaaatg ggaaaccaat ggtaggggca ttgcctatga tcacaggttc
     1201 gtttattgtg acctttttat ctgctatcgt tgccacgcca tttgccattg gtgcagcagt
     1261 attcatgacg gagatctctc ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct
     1321 gcttgtaggt attccttctg tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt
     1381 tgtcagaagt atttttggcg ggactgggtt tggtatctta gctggtacat ttgtgttgtt
     1441 cgttatgatc ttgccgacta tcacaacgat gactgttgat gcattaaagg cagtgccacg
     1501 gcactatcgg gaagcttcat tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt
     1561 cgtgctacga gcagccgtgc ccgggatttt aacagcagtc gtttttggta tggctcgtgc
     1621 gtttggtgaa gccttagcta tccaaatggt catcggtaat gcagcattga tgccaacagg
     1681 tcttactact cctgcttcta cattgacgtc catcttaaca atgggaatcg gaaatacaat
     1741 catgggaaca gttgaaaata atgtgttatg gtcattagcg ctgatcttgc tattgatgtc
     1801 attattcttt aatatcgtga tccgtatgat tgggaagaaa ggagagctga aataatgaac
     1861 gcaaaacgct cagataaaat tgctacaggt gttttatata tagtttcggg gatcatcgta
     1921 ttgattttag cagctttact actttatatc ttagttcggg gaataccgca catttcatgg
     1981 tccttcttga ccgaaccttc caaagcctac caagaaggcg gtggaatcgg gatccagcta
     2041 tttaactcga tttatttgct tttgatcacg atgatcatca gttttcctat ttctttaggt
     2101 gcgggtatct atctttcaga atatgctgga aaaaactggc taacagatgt gattcgcacg
     2161 tcgattgaaa tcttgagctc gttgccttct gtcgttgtcg gtttattcgg tttcttggtt
     2221 ttcgttattc aattccagta tggtttctcg attatttctg gtgcattggc tttgactgta
     2281 ttcaacttgc ctcttttgac tagaacagtt gaggagtctt tacaagcggt ccattacaca
     2341 caacgagaag caggattggc cttaggtttg tcacgctggg aaacagtgat aaaagtggtg
     2401 gttccagaag cgacaccagg gattttgact ggtgtgatcc taagttctgg aagaattttt
     2461 ggtgaagcag ctgcattgat ctatacggct ggacaaagtg cacctgcgtt agattttacg
     2521 aactggaatc cattaagtgt ttcaagcccg atcagcattt tccgtcaagc tgaaacatta
     2581 gctgtccata tttggaagat caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg
     2641 gctggcgctt ctgccgtgtt gattattgct gttttactgt tcaacttcgg cgctcgtgcg
     2701 attggcaaac gattataccg aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa
     2761 aatataactg gaatgaaaca aatgtcatca ccatgccaaa agagaaagaa gtagcactat
     2821 atacggatga tctacatgtc tggtatggcg acaatgaagc aatcaaagga gtggatctgg
     2881 aatttgaaaa aaataagatc actgcactga ttggtccttc cggctgcgga aaatctacgt
     2941 atcttcgttc gttgaatcga atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa
     3001 tcatgtatca aggcgtagat attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac
     3061 ggattggcat ggtttttcaa cgtcctaatc cgttcagcaa gtcgatttat gacaatatta
     3121 catttgcttt gaaacagcac ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa
     3181 caagcttgaa acaagctgcc ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct
     3241 tggctttatc tggcgggcaa cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac
     3301 ctgatatctt gctgctagac gagccggcaa gtgctttgga ccctatttcg acaggaacgg
     3361 ttgaagaaac acttgtaaac ttgaaagaag actatacgat catcatcgtg acgcataaca
     3421 tgcaacaagc agctcgtatc agcgactata ctgccttttt ctatttagga aaagtaatgg
     3481 aatatgacaa aactaacaag atatttacca gaccaaaaat tcaggcaacc gaagattatg
     3541 tgtccggtca ttttggttaa gacggaggcc tcaacatgac aaaagagatt atttcatcta
     3601 aagatcttca tctgtattat ggaaagaaag aagcactaaa agggatcgat ctaagcttca
     3661 atcaagggga aatcactgcg atgatcggtc cttctggttg cggaaaatcc acttatctgc
     3721 gttctttgaa ccggatgaat gacctgattc cgaatgtaac gatcaccgga agtgttttat
     3781 ataaagacaa agacatctat ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg
     3841 gcatggtatt ccagcagcca aacccatttc ctttttctat ctatgaaaat gtcatctatg
     3901 gattgaaatt aaaaggggaa aaagataaaa aagtccttga tcaagtagtt gaagaaagcc
     3961 tgaaggcagc gtctgtttgg gatgatgtga aagacaaatt gcataaaagt gctttatctt
     4021 tatccggtgg gcagcagcag cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa
     4081 tcatcttatt ggatgaacca acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa
     4141 gtatgctgtt agaattaaga gagcggtata cgatgatcat cgtgacccat aatatgtctc
     4201 aagcttcacg tatttcagat aagacggcat tctttttaga tggtcacttg atcgaataca
     4261 atgatacgaa gaagatattc atgaatccag aaaaacaaga gacagaagat tatatttcag
     4321 gtcgatttgg ataaggagga aacggaatgc tacgtacaca atttgaagaa gaattgctga
     4381 atctccataa tcagttctac gaaatgggga tgatggtgag cagcgccgtg cacaagtcag
     4441 ttcgtgcata catcaaacac gataaaatct tagcccaaga agttatcgaa aacgatataa
     4501 atatcaataa catggaaaca cgcttggaga agaaaagttt tgaaatgatc gcattgcaac
     4561 agccagttac aactgatttg cgtatgatca ttacggtcat gaaagctagc tcggatttag
     4621 aacgtatggc cgatcatgcg gtgtctgttg ctaaatccac gattcgcgta aaagggcaga
     4681 cgagaatacc ggaaatcgaa aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg
     4741 tggacaacgt cctagttgct tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca
     4801 atatggatca aagagtcaat gactatttca atcgaatcta taatgcaaca atcaaaaata
     4861 tgcaggaaaa tcctgagaca gtcatcagtg gaacagacta cttgaatgtt gccagttatc
     4921 tagaacggat cggagactat gtgacaaaca tctgtgaatg gatcgtctac ttggctacag
     4981 ggaaaatcac tgagttaaat acgaaccaca atgagatttt ctaaactgaa gctgtgatta
     5041 tgccgtttgt ttaggaaaag aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat
     5101 caagtaataa gaacagtcaa acaaaaaata gctcctcata tttttcgttt ggctggactt
     5161 attaccgcag atgcaagctt ttgaacaccg tttattcggg aaagggtttg tgacaaaaca
     5221 tgtcacaaac ccttttcatc atttcataga aaggataaag aatgcaaaaa aaaattagca
     5281 tcatcactat attttttatt tgtgtgctta cttcatgggt tattcagcat caagccgctt
     5341 tatatgaagt acctgtcatc gagatagaaa aagtacgaat aaaagaggat catcaattga
     5401 ttacaggaaa actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt
     5461 atcaaaaaaa tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa
     5521 tgcatggaga tgaatggcag ctagtagcaa aaaaaaatga tggctggctt ttcttcttca
     5581 caagtatctt cattggcgcc atgttgctta ttggtggaaa acagggtcta tttgctttag
     5641 gaagtctcat catcaatatc gttttacttt tacttttgtt gctcatcttt actaaaacag
     5701 aagggatttc tctattaaat atatctatcc tgttcgtcgt tatagggatg attattagca
     5761 tattggcttt agatggatgg aatagaagca gtataatcaa aatttcagct gcagttacga
     5821 gtgttttttt agcatttttt atttgtcttt tgacaatgaa tcattggaaa gaccaaggac
     5881 tgcgctatga ggaactgaat tatctgacac gaccgtatcg ttctgttttt ctttctagcg
     5941 tactgattgg aacagctgga ggaacactcg ataccgttat cacagtcatt gcgacattag
     6001 aagaattgac taagcgacaa ccagaaataa cagcgaaaca gttatggctt tcaggaaggc
     6061 aagtgggtcg agatgtgatt ggctcgatga ccaatatcct tttgttcgtc tatatcagtg
     6121 ggtcagttcc atcgcttctt ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag
     6181 aacagcattt atcattggaa tttttacgcg tcattgctgg cagcttagga atagtattat
     6241 cgatccctat ctcggtcctc ttttttctga ctgtaaggag gctgaaacga tgaatgtact
     6301 tattatactt gttgggattt tagcaatcag cgtgctactt attggcggta cgcaaggtat
     6361 gtatattctt ttaggcttat tcatcaattt agggatcttt ttcctgttgt tattcggtta
     6421 tcatcagaag tggccgatac tggcattgtc ggtcatagga tttttattga ttgctgtggt
     6481 tatcttattt tttatcaacg gttataatct aaaaatgcgt gcagcttttg tgagtatact
     6541 gatcttttta ttttgtttcc tactattgct tcctatcact gattttttag caatccaagg
     6601 ttttacatcg gtagaactag aagaattatc tggcttagat aagactttag cgattgattt
     6661 tagattactt acgagatcgt tgcttctgat tagtcttagt ggtgctgttt tagatgcgag
     6721 tgtagcaatt tctagtggga cattcgaagt atatcaggca aatccgcaat tatcttttta
     6781 ccaattgcgc cattctagtt tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac
     6841 acttcttttt gcattcatgg gaagttcttt agcgttgatc ctctggttta ttgatttaga
     6901 cattcctttt cagcaagtga tcaatgaaaa atcatttgtt ctagaatata cgatggctat
     6961 tcttaccaca ttgtccgcac tacttgttct tcctttaacc tgcgtgacta cagcatatct
     7021 ctttacaaaa aagaaagaac aactcaagat ggagtaaaag ttacaactta agtcctatga
     7081 catttttact gaatcagtgc ataatatgga tgtaaggaaa agcttgcaaa caattttgct
     7141 taaggaggca atttccatga aagaaagaga acgtatttta gatttagtga aaaaaggaat
     7201 cctttcgact gaagaaggct tagatttatt agaaagcatg gcaactgaaa aagacgaaaa
     7261 gcaaatcaaa aaagaagcag acaaggtaac tgctagtcat aaagaaaaag atcaggcaag
     7321 tcaattgatc gataaacttg aaaacggcga agaagaaatt tctgagccag ttgatccaaa
     7381 ggaaagagaa agacaagatc aagaaaactt agaaaagatc ttagatgaac tagcaactga
     7441 agcgaataaa acttcggcgc gtctagatga agtaaatgct caattcgctg acaataaggc
     7501 agcacgcaat gagaaacagg aagcattgat gcagctgaat acaaaagaag aattaggcga
     7561 gttgacagaa gaagaattag cacagcgtca gacgttagaa gcagaaatca aagaattaga
     7621 agctagtggc gatacactgt tagaagaaca attgaaatta gaagcagaat taaaagatat
     7681 ccgtaagaat caatggtctg agaaaaaaga aacgtttact gataagtttg aattgccaga
     7741 tgattggaaa gatcaagcaa cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc
     7801 tggatcacag ttaggcaaat tcttgaagaa aacattccaa actgtttcag aaacagtgaa
     7861 tgacaacatg gaatggaaag atgtcagctt acgtgtccct gggattgcga caacgaaatt
     7921 tgagcatgaa ttttactatg aagctcctgc agcaagtatc cttgatatca aggcagcaaa
     7981 cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg aaagttgaag ctaagatcaa
     8041 actttacgga aaaatgggtg ctgaaccatt tgaagcattc tctgaacgga gccagattga
     8101 ggttaatgaa gatcatatct ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt
     8161 tttctactta cctaaacgag tatacgacca cgcagctatt aaacttctaa atggcaacat
     8221 catgattgaa acattggaag ctaaagatat ttatacaaaa tctacgaatg gtaacatcat
     8281 cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt gtaaacggca atatcgatat
     8341 tcgaaacggg aacatcttag attctattat cgaaacagta aatggtacag tgacatttgg
     8401 tgcgacagca gaaaaccttt ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa
     8461 agaagataat ttgaaaaaag tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt
     8521 accagatggc atcggtttgg aaggacatgc taaaacaagt cttggaagta tcaacagccg
     8581 tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt
     8641 ccgtcgtgtt tcagatggtg aaatcgcgca agtgcaactt tcaacgacaa caggaagtat
     8701 ctatttaaaa gatacagata aataaagagg ttgtgaaaga gatgttcagc cttgagcaat
     8761 aagaagcgat tttgtaaaat agcttcccct attttacaaa attgtgcctt attgccgata
     8821 gtctatctct tgaacaccgt ttaccaagag attatgaacc tgccaattat cgccgagtca
     8881 caaggaataa tttcagaaaa agctcgtcac gttttatgaa tatggtaatt tgtgaccgag
     8941 aattttgcag aaagacaccg attaagaaga aggttgtcac aacgattttc ttcatttaaa
     9001 atagtagaac agaggtgaga catgatgaaa aaaaagttga caaaatctaa taaaaatgtt
     9061 gttttgacag ggactttagc aggaatagga gaatacatcg ggattgatcc gactgttatc
     9121 cgtgtgcttt atgtattcct gagcttagtt gcttttggaa gtccaattat tttatacatt
     9181 ttattagctt tgattatccc atttgcgcgt tctgcacgaa caaactatgg acataacaat
     9241 gcatattatg aaaaaaacaa ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa
     9301 gaagcggaaa aaatcgatga tgacgaatgg agtgactttt aatgtcgtac tttcaacgta
     9361 ttttagttaa tacgttaaca ttcatatcat tggctgttct ttttcctgcg atggtccatg
     9421 tgagcagtat ttggacggcc gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg
     9481 tcaaaccgat tttgacgata ctgtccctgc cctttacatt agtcacattg ggattgttta
     9541 cgtttgttat caatgcagcg attcttaaaa tgacctcctt ttttgtggga gcagaacatt
     9601 ttagtttttc ttcattcggt gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg
     9661 taattgtcag cgaacataac agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca
     9721 gtttttttat ttagaatcag gaatgcgttt tctattacat tggtaattaa ataaaaacgt
     9781 tttttttaat tggtagtact tttggctaga caatgataaa atgatagaga acactctaat
     9841 agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta
     9901 tctttggaaa tcgtgacagg cgacgagaaa agcttagacc gtgtcatcgt aactggggac
     9961 atctcacgcc caggactaga gttgactggt tattttaatt attattccca taatcgttta
    10021 cagctatttg gcagtaaaga aatcagtttt gcggaacgga tgatcccaga agagcgtttg
    10081 atcatcatga gaagattatg tagtgaagat atgcccgcat ttattatttc aagaggatta
    10141 ccagctccag aagaaatgat ccaggcagca aataaaaatg ggatagctgt actgcgttct
    10201 cccatctcaa cttctcgttt attaggagaa ttatccagtt atttagatgg tcgccttgcc
    10261 cctcgaacaa gtgtccacgg tgttttagtg gatgtttacg gattaggtgt actgattcaa
    10321 ggtgacagtg ggataggaaa aagtgaaaca gcgttggagt tgatcaaacg cggccatcga
    10381 ttgatcgcag acgatcgagt agatgtttat cagcaagatg aattaacggt gatcggagaa
    10441 ccgcctaaaa tcctggaaca tctgattgaa atccgaggaa tcgggatcat tgatgtaatg
    10501 aatctttttg gtgcgagtgc ggtacgtggc tctatgcaag tccagttggc tgtttatctg
    10561 gaagcatggg caaaagataa gaaatacgac cgtctgggct ctgaagacac gaccgttgaa
    10621 atcgcggaag tgggcattcc acaaataaaa atccctgtca aaacaggacg aaacgttgca
    10681 atcatcattg aagtagccgc gatgaatttc cgtgcacgta caatgggctt tgacgcaacg
    10741 aaaacatttg aagaaagatt aagtcgcttg attgaagaaa actcaggcaa cgactagcaa
    10801 ggagtgacaa tatgttagca caaatcaatc gagttgcttt tgagcttgta gggattccta
    10861 tctactggta tgcattgatc atcgtatcag gtatcattat cgctatgtgg cttagcagca
    10921 gagaggctgt tcgggtagga ttaaaagcag atgatgtgac agattttatg ttgtggggat
    10981 tgcctatctc cattattggc gctcgcttat attatgtttt atttgatctt ccccagtata
    11041 tagctgatcc aatccaaata ttcaatatcc gttcaggagg cttagctatt tatggcggac
    11101 tgattgcagg cgcgatcacg ctgtattttt ttactaaata tcattttatt tctttgtgga
    11161 cttttttaga tatcgctgca ccaagtgtat tattagcaca ggcaatcggt cgttggggaa
    11221 atttcatgaa ccacgaagca tatggaccag acacgacgcg aagcttctta gaaagtctgc
    11281 atttaccgca attcatcatt gataatatgt atatcgaagg gatctatcgt caaccaacat
    11341 ttctttatga atcactttgg agtttgattg gatttatttt gcttttgttt ttacgaaaga
    11401 aaccacacct tttgaaaaaa ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg
    11461 ggcgtttctt tattgaagga ttacgtacag acagtcttta ctttttaggt gtgatccgta
    11521 tttctcaatt attatctgct gtgttatttg tcggtacatt gattctattt atttggcgca
    11581 gaaaaaaagg aaatttaccg gattatgacc gctcattagg aaaaaatcaa caaattatct
    11641 gatttttaga gcgttaaaaa tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta
    11701 gcggttttag gaccaggctc ttggggtact gctttggcac aagtcctcgc cgaaaacgga
    11761 catgacgttc ggatttgggg acatcgtgct tcacaagtag aagaaatcaa cacccaacac
    11821 acgaatcaac gttatttacc agatcattat ctacccgatt ccatccaagc attcaccaat
    11881 atggaagatg cagtaaaaga cgcagatgcg gtgctattcg tcattccaac gaaagcaata
    11941 cgttctgttg caaaagaact agtgcctaaa ttgaatacaa agccagtgat catccatgca
    12001 agtaaaggat tagaacaggg aacacataaa aggatctctg acatcttgtt agaggaaatc
    12061 ccagcagaaa aaagaaaagc tgttgttgtt ctttctggac caagccatgc agaagaagta
    12121 gcaaaacatg acatcactac gatcacagct gcaagtacag atgcaaaagc agcaaaatat
    12181 gtacaagagc tgttcatgaa taattatttt cggatctata ccaatcctga tgtgatcggt
    12241 gtagagatgg gggcagcttt gaaaaatatt attgctattg gtgctggcgc tattcacggc
    12301 ttagggtttg gcgataatgc aaaggcagca atcatgacgc gcggattagc tgagatcagc
    12361 cgtttaggtg tagcaatggg tgccaacccg ctgaccttta tcggattaag cggaataggc
    12421 gatctagtcg ttacttgcac aagtgttcat tctaggaact ggcgcgcagg caacttgtta
    12481 ggacaagggc agcccctaga agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg
    12541 aatactaccc aagcagcgat ggaactagcc caaatgatgg aaatcgaaat gcccatcact
    12601 caaacaatct atgaagtgct gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc
    12661 atgttacgtg atggaaaaaa agaaaatgaa ttctcattaa caattttttg aaggagcgaa
    12721 tgtatatgaa agttaaaaaa gcagttattc cagcagcagg attaggaaca agatttttac
    12781 cagcaacaaa agctatggca aaggaaatgt tgccaattgt tgataaacca acaatccagt
    12841 tcatcgttga agaagcgttg aaatcaggta tcgaagacat cttgatcgtt acaggaaaag
    12901 ctaaacgacc aatcgaagat cactttgatt caaatattga attagaaaca aacctaagtg
    12961 aaaaaggaaa gaccgaatta ttgaaattag tagaagaaac gacagatgtc aacttgcatt
    13021 tcatccgtca atctcatcca aagggtttag gacatgccgt tcttcaagca agagccttta
    13081 ttggaaatga accatttgtc gttatgctag gcgatgatat aatggaagac gaagtaccat
    13141 tgacacagca attaatcgat gattacgaac gtactcatgc ttctacgatc gccgttatgc
    13201 aagttcctca tgatgaaact tcaaaatacg gaattatcaa tccaggagaa gtattggaag
    13261 atgggttata caatgtcaaa aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg
    13321 atttagctat tattggacgt tacttattga ctccagaaat ttttgatgta ttgtccgagc
    13381 aacaaccagg agccggaaat gaaatccaac taactgacgc aatcgataca ttgaataaaa
    13441 cacaacgcgt atttgcccgt aaattcacag gtaaacgtta cgatgtcggt gacaaatttg
    13501 gttttatgaa aacaagtatt gaatatggtc taacacatcc agaagtaggt gcacctttac
    13561 gtgagtacat cctgtcttta ggtaaaacat tagaagcaga aggaagcaag atcgatcata
    13621 atccaaaagg aataacagaa atcaaagaag aaaagaaaaa ataacccttt tttctaacga
    13681 gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca
    13741 cgaaaatgct atccttatag aaaagagaga caagaaggga gtttgatcat gtcaggagga
    13801 gaaattgcag gattgatcgc agcaatcgcc tttgcgatac ttgtcgtttt tatcgttcgt
    13861 gtgttgttgc aagtttctaa aactttagaa aaagtagatc agactgtagc agaagcaaat
    13921 acgacgatcg aaatcgtcac aaaagacgta gatcttttgt ctagacaagt agaaggctta
    13981 ctggtcaaaa gtaatgaact gctcttggat atcaatggaa aagtcgcaac gatcgatcca
    14041 ttgttcacag cagttgctga tttaagtgaa agtgtatcag aacttaatca ttctagtaaa
    14101 aacatcgcga cgaaagtcgg cggaatcggt aaaacgacag ctaaagcgac ggttgccgga
    14161 aaagtcggtg ggacagcgat gaaatttttt aaaaacaaaa aacatactga aacgacagga
    14221 ggaaaataaa aatggctaaa aaaggtggat ttttattagg tgcagtagtc ggaggcacag
    14281 ccgcagcaat cgccgcgtta ttgttagcac caaaatctgg taaagaactt cgggatgaat
    14341 tagctgccca agcggatgat ttgatggata tggcttcaga ttacacagat atggcgaaag
    14401 aaaaatcgac tgagttaaca ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag
    14461 caggtgatct agcaggtagt gtcaaacaaa aagtaagtga atcagcagat gaagcaggaa
    14521 gtgaatctga cgatatcttg ttaaatctta aagaacaatc agctgactta tctaatcgtt
    14581 tcaaaaaaac ggcagaagat atgaaaaaac ctgcagatga attaggaagt attgctaaag
    14641 atacttcaga agatatcatg attgatgtaa aagacgctgc tggtaaagca ggagatactg
    14701 tcaatgaagg aatgtcagaa gcaaaaccag ttgcagatga ctcaaaagaa atgatgaaaa
    14761 tggcaaaaga agatgcgaaa gaagctaaaa aagaaataaa agatacaatg acagattcat
    14821 ctacaaatca ctaaaatcac aaatttatag aataaaaagg tgggagcgga ctagctctca
    14881 ccttttttac tcttattttt ctaaaacaag taattctttt ggtgtagtcg tgaatggcac
    14941 acagccatcg cttgtcacat atacacagtc ttcgatacgt acccctactt tttcaggaat
    15001 atagattcct ggttcaagag agaagcacat cccttcttcg attactaagt catttccttc
    15061 tacaagggaa gggaattcgt ggacggttgt tccgatcccg tgacctaaac ggtgattgaa
    15121 atattcgccg tagccatagc tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc
    15181 tgtcacacct ggacggacgg cttcttgtgc ggcaagctgt gcttcaagag tgatgttgta
    15241 gattttttct tggaagtcgg ttggtttttg ataagcaatt gtacgagtag catcactgca
    15301 atagccattc caaacaacgc caagatcgaa taacacaagt tcattcggtg aaatcttcgt
    15361 atttcctggt gtaccatgag ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa
    15421 agacatttgt gaaacacctt gttttttcaa ttggtattca atttctgcaa tgatcgcttg
    15481 ttcagtcacg ccttctttca ctgctttaaa gccaatctca aatgcaacat cggcccattt
    15541 accagcttcg atcagtttgt ctttttcctc tagagttttg atcagttgta gacgttggat
    15601 gatggaagtt agatcagtgt aagaatccgt tcccgtcaag atatcagaga tagcatcgaa
    15661 gcgctcgaca gtcaaggcgt tcttttcaat ccctacattg ccaggctgac catagcgagc
    15721 acgaagttct tttccgatga tctcccaagg gttttcgctg tctaaatagc cacggacatc
    15781 atacgcccaa ctactgtttt gagcatcttc aacttccaaa gcaggagtga aaaggaaagg
    15841 atcttgttca agagggataa aaagagctag tacacgctca tgaggatcac tttcgtatcc
    15901 ggaaaagtaa gcgatgtggc cagggtcgct gatataagcc aattcgactt ggtgttgtgc
    15961 catccattga cgtagttcat tgatttttac gttattcatt tggcaaccta ctttctaaat
    16021 taattttgta ttcaataatt gtaccactga tttattaaaa gaccaacgtt ctaaaaactg
    16081 aaaaaagtaa aaattttcat gaaaacagag ataaaacggt tgcaattctt gagattattt
    16141 gatattctat tttagaaatg taagacgtat tttcagaaaa catataaaat atgaatagga
    16201 gaacacaaat ggaaaagcaa acaatcacta tctatgatgt agcgagagag gcaaatgttt
    16261 cgatggctac tgtttcacgt gtcgtaaatg gcaatccaaa cgttaaacca gcaacaagaa
    16321 aaaaagtctt ggaagttatc gatcgcttgg attatcgtcc aaacgcagtt gcgcgtggct
    16381 tggcaagtaa gaaaacaaca acagttggtg taatcatccc tgatgtcagc aatatgttct
    16441 ttgcttcttt agcacgtggt atcgatgatg tggcaacaat gtacaaatac aatattatct
    16501 tagcaaattc agatggcaac gaccaaaaag aagtcaatgt attgaataac ttactggcta
    16561 agcaagtgga cggcgtgatc ttcatgggtc accatatcac agacgaaatc cgtggagaat
    16621 tttcacgttc aaaaactcct gttgtattag ctggctctat tgatccagat gaacaagtag
    16681 gtagcgtaaa cattgattat gtatccgcaa caaaagatgc agtaaacaaa ttggcaaaaa
    16741 gcggcaataa aaaaatcgct tttgtcagtg gtgcgttgat cgatcccatc aatggacaaa
    16801 atcgtctgaa aggctataaa gaagcattaa gcgaaaataa cctaccatat agcgaaggat
    16861 tgatctttga agcacaatac aacttcaaag atggtctatc tctagtcgat cggatccata
    16921 atagcggtgc aacagctgtc tatgtttcag atgatgaatt agccatcggt attttggatg
    16981 gtttattaga ccgtggcgtg aaagtaccgg aagaattcga aatcatcaca agtaacaatt
    17041 cactactgac agaagtagct cgtcctcgtt taacaagtat cacacaacca ttatacgatt
    17101 taggggcggt atctatgcgc ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa
    17161 aaacgattat cttgccttat ggaatcgaag aaaaaggatc tacaaaataa taaaaggaca
    17221 accattcaga ggttgtgtgc ctgccgttta actctgcgtt acaaggagca atttcaaaaa
    17281 acagcatctc atgtttgatg aatattgtga ctcgtgattc ggagttggca ggagaacacc
    17341 gtttatttgg gaaaaggggt tatgacaaaa ttttgttata atcctttttt tgtgtcttaa
    17401 aaaagagaga aagtggttga aattacattt atcgtatata aaacaatccg tgtatagatg
    17461 ctgcattttg ttcaccacca tcaaaaaaat aagggactgt gacaatacta ttgtcacaat
    17521 cccttattac tgtgtattct attaaatcaa tcatctttct catctgattc tttagttgct
    17581 tgattcgctc ttgcataagc tgaagcggtg ttccagtaat ctgtatagtt ttcatcggta
    17641 ccgccaacgc caaatttaaa ggcgcttttc ttcggtccat ttttcgccca tagcgattca
    17701 actgtttttc ctggtgtttg gatggatttt ttattgacag taacttttcc aggtgtttga
    17761 ccagtaaatc cagatacttt tttcttcatc acatcagatg ataattcgaa tttctcatca
    17821 gtgccaaaaa tgtcaggttt tgcttggtaa gcttgattga tcagattggc aagataatta
    17881 gccgaacgta tccttgcttt gctgtccatt ccagtattgt catcgtgccc tgcccagcta
    17941 ctgatggtaa tagacggagt agagacaatt agccatgaat ctcggtattc attggtagaa
    18001 ccagtttttc cgacccaatc tgcttttcct agatttccgt ttaagcttga gatggcatct
    18061 ttgaatggtg tagtgatttt tgcattgatc acactacgca tcatgtcatt catgatcgat
    18121 gccgttgctt ccgaatagat ccggacagag ttgctttcgt gtttataaat tacatttcct
    18181 gcgttatctg tgatggaatc gatgatatag ccttcttcat agacgccgcc gtttgctaaa
    18241 gcctgaaatc cgttggtctg ttgaagcgtc gtgacatcta ctgtacctaa aggagcagat
    18301 tcaacgcccc aattgtcatt tgccgggtag ttcatctttg ccaagtagtg ctcgtaagca
    18361 tattgtttcg atccaccgga attcaacaca tcttgatata agtgataagc cgggatattg
    18421 ttggaccatt ctagtgactc acggacggtt tggaaggtgt tcgatccttc gttagtagca
    18481 ttgacgattt tctcgccagc attttctcct tcttgccaag ttgttgcata atctgataca
    18541 cgggattctg atccgataag ccccatgtcg attgcaggtc cataaacaag aactggtttg
    18601 attgatgagc cagcttgacg ttctgtatcg aaggcatggt tattttgatt ctgactgtaa
    18661 ttccgtccgc caacgaatcc gtagatgcgg ccagttctgt tatccatcaa tacatttccg
    18721 gtttctactt gactgccagc accatcatct aacatgtatc cataattttc aacgccattt
    18781 tgcaatgcag catagacatc tttatcgatt gtcgaatgga ttgtatatcc tttattttgg
    18841 atcgtttgct gtgccttttg ataataagcg tcactgaccg atgaatcaga catatcagcg
    18901 ttgtcttttt cagccaattg ttgtgcaatg attcttgttg cttcattcat cacagtataa
    18961 tacaagaatt ctctgtcagt ctgttctgcg atttgttgtg gtagaaagtc ttttgtaagg
    19021 tcataggctt ttgcttcttc gtattgttct ttcgtaattt ggccttcacg atacatactg
    19081 aataaaacaa aatcttttct ggcaaggccg gcagaaagat cttcttttag cgcgcctgtg
    19141 ttcgtgtatg gactatacgt gatcgggctt tggggtaatc cagcgatata agcagcttgc
    19201 ggcagcgtca catcttttgc atctacacca aagattcctt ttgcagcttc ctggactcca
    19261 gcaatgtttt gtcccttatt attacgacca aatggtgaga cattcaaata cgtagcaatg
    19321 atctcatctt ttgaaaagta tttttctact tgtgcagaaa gaatgatctc gtttgcttta
    19381 cgtttgaatg ttgtttcatc tgtaaggatc tgctgtttga ctaattgctg tgtcaaagtt
    19441 gatccaccag aagagccaat accagtagct tcagatacta atgctcgtaa aacagcttta
    19501 ggaacgtaac cttggtgttt atcgaagtat tcatcctctg tgctgataat tgcagttttc
    19561 aataaaggtg aaatcttatc agatgaaata gtcgtgcgca tcagatctgt ctggattttt
    19621 gatatctctg tattatctgc ataaacaatc tttgacgttt ctgtaatgtt tcctagttct
    19681 gtctgcagtg cttttttagt cggcaattgt gtgtcttcga cgagataggc aaaatagcca
    19741 gcgcctattc caatacctag agctgcgaac atcaaaagaa gaacagtaat aaaaacagtc
    19801 aatgattgaa aaactctaaa aataatctta ggaacaaacc atttttcttt ttttcttcta
    19861 ttctgttttt ttcgagtatt tttgcgtggc aaaagggtca cctcaactaa tggttttatt
    19921 tcgctaatta tagcaaataa tagaacaaaa aaacagacaa ggctgttttt ggcaggaatt
    19981 tcttttttaa aagagttaaa acgtgagaaa atgatcttta ttataaaaga gttcttaagc
    20041 ttttctttag agaaaatttc agttactatg gagaacaggg caaagaaaaa tcaatcattt
    20101 tctttgtata tcatcttacc aatatatagg aattgttagt aacaattgcc tttcaaggag
    20161 gaatccatcc atgttattag gctcacacgt cagcatgagc ggaaagaaaa tgctgttagg
    20221 agcagcagca gaagcagcca gttatggatc ttcgactttt atgatctata caggcgcccc
    20281 tcaaaacact agaagaaaag ccatcgaaga gatgaatatt gatgccggaa aagcgtacat
    20341 ggcagaaaac ggtctaacaa atatcgtcgt tcatgcccct tatatcatta atttggggaa
    20401 tacgatcaaa ccagaaatgt ttccttttgc cgtccaattt ctgagagaag agattgttcg
    20461 tgcggaagct ttaggcgcta ctcaaattac acttcaccca ggtgctcatg taggggcagg
    20521 aacagaagcc ggaattgcta gaattattga aggcttgaat gaagttttga caaaagaaca
    20581 gacggcacaa atcgctttgg agacaatggc aggtaaaggt acggaaatcg gtcgaacatt
    20641 tgaggaatta gcgcaaatca tcgatggagt aaaattaaat gaaaaattat ccgttacttt
    20701 tgacacctgt catacgaatg atgccggtta taatattaaa gaagactttt caggtgtaat
    20761 ggaagagttt gataagatca tcgggttaga tcggcttaaa gtactccata tcaatgattc
    20821 taaaaatccg caaggttcgc ataaagaccg gcacgccaat attggttttg gaacaatcgg
    20881 attcgacacg ctgaataaga tcgtccatga tccaatgttt gacaatattt cgaaaatctt
    20941 ggaaactccc tatgtcggca aagataaaaa gaaagcttac gcaccatatg gaaaagaaat
    21001 cgccatgttc aaagcacaaa tattcgatcc agaagtgttt cctgagttat tagcagaaga
    21061 agtgatactc taagattttc tatctgacag cagcaaaaaa ggaccaggca cattgccttg
    21121 tccttttttg tttaagaaac aaaggagtac tttttgcagt ctttttcatc atgcatcttc
    21181 tcccccctct ttttaaattt agacaaacaa ggtatactgt tagaaaagta aaaaaacgaa
    21241 aagtttcaca agcaagtggc aaaacgtgat tggaaggacg aaaagatgct gaacacttta
    21301 aaacccttga tcaaccaact aaaaaagaaa catacatact ttattcttgg catagcccta
    21361 ttatttacaa ttattcgttt gtatttatat agtaaagcaa tctatggaac agacatgtcg
    21421 gcaacctatg atgaccagct gctgattcgc tatgctcaaa atattttgga cggtaaatgg
    21481 ctaggggagt atacgactac aacactttcc aaaggaatct cctacagctt atttttagta
    21541 cttgcacata aactgcatct ttcttatgct gttttgttcg gcatgttcag tatttttgcc
    21601 agtatattgg ttgcactcgc gtttaaaccg atcgtgaaga acaagtatct tcttttaagt
    21661 atctattttt attttttgtt ttctccgatt agttttacac atgaatattc cactcgaaca
    21721 tatcgtaatg ccctcgtgat tccggctgtt gagatcatta tcgcttgttt actagctatt
    21781 tattaccgca aatacggttc tagcagaaaa ttgctgccat ggtttttagg gctttctgtc
    21841 gcttttcctt tcttttggtt cattcgcgaa gattctatct ggcttcttcc ttttactgta
    21901 gtagctttgg gtatcagttt cgtccagatc ttgctagggc aagacttttc tttcaaagaa
    21961 gtaaagaagt taaaaaaacg actctttctt ctctcgaaac gggaatggct gaaactttca
    22021 ttgtttttac ttcctatagt tggcttttcc atcgtctatc agtggatcga atataaaaat
    22081 gagacaacat acggtattgc aaccatcaat gaccgttcca gtggagaatt tggacgtttg
    22141 atgaaacaac tgatccgaat agatgatgaa acgaacttga ataaagaaaa cagtgagata
    22201 tgggtctcac atgctgcact aaataaagca ttagccgcat ctcctacatt ccatactgtt
    22261 tctacccgta ttgacgaatt atatacgact cacgcgtgga ctcaaggtgg aaaagtaaaa
    22321 gaattacccg gagatatcat cttttgggca ttgcgagatg tggtaagcga atctggttat
    22381 taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa aagtcaacca agaattagaa
    22441 gaagcctatc acaagggaac catcaaaaag aaaaaagagt tctatctgat gggatcagga
    22501 gatggaaaga cgatgagtga tctgccaatc gtattcgatt tccttcaatc aggatacgct
    22561 tatgcatggc gatataaaga tttccatcaa ggaggaaatt atagtttcgg aacgcaagaa
    22621 caagtcaaac aggctcaaga attattaaac cttccgctaa tggagaattg gatcgatagt
    22681 aatgaaccga gaacacctaa tttccaatta acaaaatcag caaaagtcgc gaatgtcatc
    22741 ataaaaattt accaagttct ggctattccc gtgctgattg cttcaatagc agggatatta
    22801 cttatgatac taggaaccgt atttgcaaaa gctgatcgtg cctattatag ttcctctttg
    22861 attgtggtca ccggattaat actgacacaa ttggtttttc ttttcggtgt ctcgtggttt
    22921 tgttcttatg cgccagaaaa aaaggattat tttttaagtg tctacactgg cgcaggcgtg
    22981 ccattaacgc aattcatcgt agtaacaagc atgctagttt tcagtaaatt aaatctgaaa
    23041 aaggctttat cttctaaata aaaagaccct tggtagctag atttttaacc taactatcaa
    23101 gggtctttgt gtggaaaaat cagtttctat tcgatttctt ctaccgtttc ctgcaaacgc
    23161 tgttcatatt ttttgtttcc tttatataaa tcaccaatcg tccatgccaa caggagaagg
    23221 acaagaacaa tgattacata agcgatgtga tcagccaatg ctagttgagc agaagtggct
    23281 tgttcaccaa agaaactttc gatctgtcct ggtaatcctc gtaagttcaa gtaagttaga
    23341 ctaatcacag agaaccagcc aagaagtttt acgatgaatg aatttttaaa tcggctaccc
    23401 atctccgctt ggctgttggt catcatcagt aaagggatca tcgaaaacgg caacgcaaaa
    23461 gccaagaaca cctgggattc attcatcaag ttattcaaag cggtatgttc ttcgatcgtt
    23521 ccttttgtac ttgtaaacaa aacacaaatc aatacaggaa tcacagaaag caatcgtgtg
    23581 accagtcttc ttagccaaat cgggatacgc atatggatga agccttccat gatgacttga
    23641 ccggtcaacg taccagtaat agtagagttc tggccagaag caagaagtgc tacagcaaat
    23701 aaagtggata agataccaga ttttgctaca gcaatcaaga taccattgct catagctgat
    23761 ggatcagata aggcttcata cagaccaaaa aaggaaggat cttggacaga accggttttg
    23821 aagacagcga cacccatgat caatagcagt gcattgacta gaaaagccat cgacaattgg
    23881 atattagagt cccaagtaga aaaacgtaca gcttgtgcta catcttcttc gtcttggtga
    23941 tcaatcttcc gagtttgaga aactgctgaa tgaagatata agttatgcgg catgactgtt
    24001 gcaccaataa tccctaaagc tccagtcagc ggagtttcgc cgccgatgac cggtgagtct
    24061 gagaatgtat gactgttagg gataaagcca gcaaataaac tgtgccaatc tgggttagac
    24121 aagccaactt gatagacaaa aacgaataaa atgacgaaaa ttaagcagac aaccagtgct
    24181 tcgatcttac gaaacccgac ttttgtcagt agtaagagaa cgaaaacatc taatacagta
    24241 ataaagacgg caatcaccaa tggaatatca aaaagtaagt agagggcgat tgctgcacca
    24301 atgacttctg cgatgtctgt tgccataatg gctaattcag tcaagatcca taaaatgaca
    24361 cccaatgtct tacttgtgcg tgctcggatt gcttgggcca aatccatttg ggtcacgatt
    24421 cctaattttg ctgccatata ctgtaaaagc atagcaatca gactagacat caaaataact
    24481 gacatcaata aatattggaa attttgtcca ccagtgatcg aagtggacca gttacctgga
    24541 tccatataac caactgctac taatgcacct ggtccagagt aagcaagaag tgttttaaaa
    24601 aaacttttcc cctttggtac atcaactgtt ccatttactt cagctaaaga taaaccattt
    24661 gtatgttcaa taagtcgatg cttatgaaat tgtttgttgg attgattatt caagaaaaaa
    24721 ccacctttcc tatttatcta cgattacatt ctacacctca aaaagaaaaa attaaagaaa
    24781 aatattcggt atgcctaatt tattttcgtg gcgcgtcttg ttagatgacg aaagaaattt
    24841 tgttctcctc taaaaaaacc actatttttt gcatgaaact aaagaatacg cctttctttt
    24901 ttcaataaac tttagtaaaa taagtcattg agagcaattc ggaaatagaa aggagagatt
    24961 tatatgttat caacaggtat tgtcgtactt attgctatta ttgctttgct aatcggcgca
    25021 gtaggtggct ttttcctagc gcgtaaatat atgaaagact atttagaaaa aaatcctccc
    25081 gttaatgagg acatgttgcg atcaatgatg atgtcaatgg gacaaaaacc atctgagaag
    25141 aaaattcgcc agatgatgca gcaaatgaag aaccaaggga aaaaataggt tctgacccaa
    25201 cgacttttta cgggctgatt ttttcagtcc gttttttatt ttgtaatttg ttagactatt
    25261 cagattattc agcaaaaaga caagcacaga caatcctagg ctcttgataa agacaaagga
    25321 ggagtaaaat gtcgattttt agaaagttag gctggttttt tcgtcaggaa aagaaacatt
    25381 atttaattgg ggttttctcg ctattcgcag tggccttggt acagttagtt cctccaaaag
    25441 tgattgggat catcattgat gagattgccg ataaaaattt atcagtaaag atcattttcg
    25501 ggtggatctt cctattgatt atcgctgctt tcttacaata tctttttcgt tatatctggc
    25561 ggacgaatat ttggggaagt gcagctcggt tggagaaaga acttcggcag cagttattca
    25621 aacattttac aaaaatggac aatctctttt accaaaaata tcggactggg gatttgatgg
    25681 ctcatgcaac gaatgatttg aatgcgatcc agaatgtagc aggagctggt atcttgacct
    25741 ttgcagattc agtgattacg ggcggaacaa cgatcattgt gatgattttg tttattgatt
    25801 ggcgcttaac actgattgcg ctgattcctc tgcctttgct ggccgtcact tcacgagtgc
    25861 tggggtcgaa actccatgat gcttttcgtg attcgcaaga agctttttct agtatcaatg
    25921 ataagacaca agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag
    25981 aagatctagc ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca
    26041 attttctaga ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg
    26101 caacaattat tttaggtgga aagtatgtct tggaaggcac gatctcgatc gggcagatgg
    26161 tctcatttat tagttatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt
    26221 tcaacgtatt ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa
    26281 aaacgcatat cgtggaacaa ctgggtgcag tgcagacccc ggtaaaaggc gagctgaatg
    26341 tagctatttc ctcttttaat tatccgaaag aagaatcacc agcattagaa caaatcaagt
    26401 ttgcaatcca agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacaa
    26461 cgatcatcaa attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg
    26521 gacgttcgat caaagaatat tcattgaatg ctttattagg atcaatcggc tatgtgcctc
    26581 aggatcactt cttgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac
    26641 tttcagaaga agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg
    26701 aattaccaaa aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac
    26761 agaaacaaag gatttcgatt gctcgagcat tgatcaaaaa tcctgaatta ttaatattag
    26821 atgatgcact ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag
    26881 aaataagaaa agagaaaaca actatcattg cagctcaccg tttaagtagc gtgatgcatg
    26941 caaaagaaat cattgttgtc gatcaaggga aaatcattga acgaggaacc catcatgagc
    27001 tgcttgcact aggcggctgg tatgaacaaa tgtgggagaa acagcaatta gaagcgaaga
    27061 tagaaggagg cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc
    27121 acttaaagaa cagaaaaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaac
    27181 aacgttcatc gttgctatct tatttgcgtt tgctctgtct gtgatcaatg tcctattgcc
    27241 aaggatcatc caaactttta tggatgatca tctggctaaa cagtcggcta cgacacaagt
    27301 tattttgttt tttgctggta tgtacttatt tggagtccta gtaaaaagta tcatttggtt
    27361 tttccaatgg tatttgtatt ccatggcttc attgaagacc tatcagtata ttagggtaaa
    27421 attatttgaa aagctgcata cactaggtat gcgttatttt gatcagacac cggcaggatc
    27481 aaccgtttct cgagtgacga atgacacaga aacgctattt gaattttggt acgtcttttt
    27541 gatggtattg actgggatct ttgcagtcgt ttcttcattc attgccatgt ttcaaatcaa
    27601 tggagaaatt gccttgtgga ctttggtgtt cttgccgatt ttaggaattg tcatctggta
    27661 ttaccagaaa tttagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa
    27721 tacaaaattg aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc
    27781 tcgtttagct aaagaatttg aacaagtgaa caatgagtat ttagacacgc gctatgcgat
    27841 gatcaaaacg aattcgattc tgttaagtcc gatcatcaat tttctatatg cattggcgat
    27901 tgcgttggcg ttgactttat ttgggatcga tgcgttgcat tctcccgtag aagttggact
    27961 gatctatgca tttacgactt atgtccaggg attttttaat ccaatgtctc aaatgatgga
    28021 ctttttgagc atcttcacag atgggatggt tgctggtagt cgtatcttga agattatgga
    28081 tacggaagaa tatgcaccac aacaaaaccc tggagcagat caaatgatta cttcaggtaa
    28141 gattgagttt cgcaatgtaa gcttttctta tgatggagag cacaatgtat taaacaatat
    28201 ctcttttgtt gctaatccgg gagaaaccgt tgcattagtg ggacacaccg gaagtgggaa
    28261 aagctcgatc atcaatgtgt tgatgcggtt ttacgaattt tatgaaggtc agatattgat
    28321 tgatgatcat gacattcgag aatatccaat ggaagagtta cgtaagaaaa tggggctggt
    28381 cttgcaagat gctttcttgt tttatgggga tatcgcagga aatattcgga tgatgaatcc
    28441 agctatcaca gacgaacaga tacgccatgc ggctgagttt gttcaagcaa acaaattcat
    28501 cgatgaatta ccaaaaggat atcactcaaa agtgatcgaa catggtgcca gctattctag
    28561 tggacaacga cagctgatca cttttgcgcg aacaatcgtg actgatccta aaattctagt
    28621 attggatgaa gcaacagcaa atatcgatac tgaaacagaa ggattgatcc aagaaggatt
    28681 ggcgaaaatg cgacaaggac ggacaacgat tgcgattgcc catcggctgt caacgattcg
    28741 agatgcagaa ttgattttag ttttagacaa aggtcatata gtagaacgcg ggaatcatga
    28801 aacacttcta gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gtagtggtat
    28861 ataaaagatg ttgtgacagt agcattttga cctaagcact agagtgaaaa taaaacatag
    28921 cttcccatat ttttcatctg tgagagttag tgtcgtagtt accagattta aaaaagacga
    28981 cgtcagctag tcagaagtgc ctaatagccc ctaagaaagc gttgacaaaa tcgtttgctt
    29041 tcgataatat aaagaaaacg atacaacttg gatagtgatt gcctgatgca agctaaacct
    29101 gatgagtctc ccatggtttc atggacggct ttttgggttt ttctttttgt tttggataca
    29161 gccctatata tcatggaaag gagcaaaaca tgctgattga gaaagtactg aataacaacg
    29221 tcgtaataac aagggatgaa agagatcggg aaatgattgt gatgggaaaa ggattggcat
    29281 tcaaaaagaa ggtgggtgac ccgattcttg actcatgcat cgacaaaaaa tttcagttga
    29341 caaatgacgg actttcacaa cagttccaag aattggcaca agcgattcct cttgagtact
    29401 tagaaatttc ttgtgaaatc attgaatata caaaagaggc tctgcaagtc catttaaatg
    29461 attctgtgta tatttcattg acggatcact tgtatacagc tatcgaacgt caaaaaatgg
    29521 ggatctttgt gccaaatgta ttgctgtatg atatcaaaca actgtttcct aaagagtatg
    29581 ccattgggaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    29641 acgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccagcaag
    29701 acaataccta cctgtttacc acaacaatac aggatatttt aaatatcgtc cgctattact
    29761 ataagattac atttgatgaa gattcagtcc acttttttcg attcgtcaca cacctgcgtt
    29821 tttttctgac acgtgtccta gatggagcga aatcagaaga tggagaagtt tctgaggaat
    29881 tgttccaagt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    29941 actttttaac cggggctttc gactaccaga tgtccacgga tgaaaaaatg tatttaacga
    30001 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaag gaggacaaat
    30061 taacaagaac gtatgaaata agggtagtga cattcagttg gctaaacctt ccactaatta
    30121 attggaggga atagaaatgg gaaaatacca ggaattagca gaaaacatcg taaagaatat
    30181 cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg acgcgacttc gtttcaaact
    30241 aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag atggaaggga tagtcaccat
    30301 catgaaaagc ggcgggcaat atcaagttgt aatcgggaat catgtgccag atgtctatgc
    30361 agaagtactg acagtagccg ggatttccga tgaagcagaa gaagaggttt cttcaagtag
    30421 taatctgttc aatcgattaa tcgacatatt aagtggctgt ttccagcctt ttttaggtgc
    30481 tttggctgca gcgggaatgg tcaaaggatt aaatgctttg ttcttgtttc tcaaatttta
    30541 tactgaagga tcgggtacgt acatcatgct gaatgggata ggggatgcaa ttttttattt
    30601 catgccagtg atcttagggt acacagcagc gaaaaaattt catctgaatc cgatggtggg
    30661 gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa ggagcagcac tacaaagtgg
    30721 tttccctgat ggcagtcctg cgccttatag cttgctgggt cttccggctt atgaaacatt
    30781 tttgggtatc ccatgggtgg gcagcagtta tactagtagc gtcgtacctg ttatcttcat
    30841 tatcgcattt gccggacaag tccaaaaaat tgccaagaaa tggattcctg cagtcgtcca
    30901 gacctttttg gtttcttttt ctgtgttatt aatcgcactt cctgctggct ttttgattat
    30961 cggtccgatt gtcagtatac tgacagatct attaagcacg ggattccaag cattgatgga
    31021 cttttctcca gctgtgtatg gtgcagttct agggtttctt tggcaagtac tggtgatttt
    31081 cggacttcat tggagtatta tccctcttgc gattatgcag ctagcgcaaa atgggtggag
    31141 tcagatgtta gtaggcgttt atggcgcgag ttttgcacag acagcagtgg tattggcgat
    31201 gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc atgccagctg tgatttcagg
    31261 aatcttcggc gtaacagaac cggctattta tggaatcacc ttaccgaaga agaagccatt
    31321 tatttactcg atgattggtg gtgctgtttc aggaatctat ttgatggcat ctggaacaat
    31381 cagctacata atgggcggat tagggatttt tggggtggta aactacatca cggaaacaga
    31441 cgccagtggt atgtttcaag cgtttatcgg cattgctatt gcttcggtaa tcgggttttc
    31501 gctgactttt ttcttttgga aggatgaagc aggtgaaaca gatgctatac agaaaaaatc
    31561 aggtaaagaa attgtaaaaa caccgataca aggacaaatc gctccactaa atgcagcgaa
    31621 ggatgcagct tttgcacaag gaacactagg tcgaggaatc ttaatctacc ctgaaaaagg
    31681 agaagtacgt gccccttttg acggaacagt tatgaccctg ttcccgacga aacatgcaat
    31741 cggaatggtt tctgaaacag ggctggaatt gctgattcat gtcggattgg atactgtgca
    31801 gttggaaggg aaatattttg aaagcttagt gcagcaagga gaaagagtga gacaaggcga
    31861 tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa ggatacagtt tagaaacacc
    31921 tgtgatcgtc accaattaca cggattattt ggatattttg gaaaaccaag gggagacggt
    31981 cgggccagac gatgcactta tcaccgcact gacctgaacg gttaggtgaa gaatggacta
    32041 ggcttaacta ttctttttat tccacttctt tattttcaaa attgaaaggc tctttcatga
    32101 tggctgagct gatagggcgt ttatcgaatt tttgaccgac agaaattaga tggactaaat
    32161 tctgttgata attttctgca aaaacacaac tatataacac gtctagaaga taacaaattg
    32221 atgtattgat cgtaaaatta ccaatttttg aatacagtcg ctcgcgtgtc gtaagtcgca
    32281 aagcggctgt actcattgca gatagcgttg aatcaccgat actggtcaaa gctaaaattg
    32341 gaacatcatt ttgtttcaat atttctgcgg tacgaaggat cacattgttt tcacccgtat
    32401 aagaaatcag aatcccgcac atatcgctcg tacaattgta cgcttcatag aatgattcac
    32461 caaaagtggt gacaattgtg acatccattt tgatgcgctt cattttcagc atgaaatctt
    32521 gagaaatcaa agtattcgag ttactggcga agatcacgat tttttttgcc tggagcagta
    32581 aatatttagc tttttgcagt tggtcatgat gaatgagaga aagtgtatca tgaatcgtca
    32641 tctgttctaa agaagccatt ttatttgcga ttgtcataat accgtctgtg gtggaaaaag
    32701 gaagattcgc atctacgtct tgaaaattcc cttcaagata ggctgtttca gctaaaaaag
    32761 cattacgaaa actgacccag ccatcaaaac ccattttttt tgctacacgg atgagtgtag
    32821 acgggtggac ataacatcgc tcagctatcg cttggatggt catttcgcca agtgctggag
    32881 aatcgcttaa gagaaactgg atcacttcat tttctgaagg agaaaaggag atttctttga
    32941 ttttttcacg taataacatg tagatacctt cttcttattt ttttacgtaa attataactt
    33001 ttttg
//
LOCUS       DAZUKA010000012        32700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000012 DAZUKA010000000
VERSION     DAZUKA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.012"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8PN_000471"
     CDS             <1..734
                     /locus_tag="ACG8PN_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860651.1"
                     /translation="FLFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKR
                     LVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPF
                     ILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTI
                     LTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQ
                     SVVGFVLLMITNTIARRFDSDSALF"
     gene            746..1666
                     /locus_tag="ACG8PN_000472"
     CDS             746..1666
                     /locus_tag="ACG8PN_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860652.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1707..3164
                     /locus_tag="ACG8PN_000473"
     CDS             1707..3164
                     /locus_tag="ACG8PN_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4860653.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3509..4123
                     /locus_tag="ACG8PN_000474"
     CDS             3509..4123
                     /locus_tag="ACG8PN_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4860654.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4116..5840
                     /locus_tag="ACG8PN_000475"
     CDS             4116..5840
                     /locus_tag="ACG8PN_000475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4860655.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5837..7288
                     /locus_tag="ACG8PN_000476"
     CDS             5837..7288
                     /locus_tag="ACG8PN_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4860656.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7761..9914)
                     /locus_tag="ACG8PN_000477"
     CDS             complement(7761..9914)
                     /locus_tag="ACG8PN_000477"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4860657.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9916..10962)
                     /locus_tag="ACG8PN_000478"
     CDS             complement(9916..10962)
                     /locus_tag="ACG8PN_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4860658.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11112..12401
                     /locus_tag="ACG8PN_000479"
     CDS             11112..12401
                     /locus_tag="ACG8PN_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4860659.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12414..15116
                     /locus_tag="ACG8PN_000480"
     CDS             12414..15116
                     /locus_tag="ACG8PN_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4860660.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15101..15958
                     /locus_tag="ACG8PN_000481"
     CDS             15101..15958
                     /locus_tag="ACG8PN_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4860661.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(16029..16886)
                     /locus_tag="ACG8PN_000482"
     CDS             complement(16029..16886)
                     /locus_tag="ACG8PN_000482"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4860662.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17097..19268
                     /locus_tag="ACG8PN_000483"
     CDS             17097..19268
                     /locus_tag="ACG8PN_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4860663.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19336..19677
                     /locus_tag="ACG8PN_000484"
     CDS             19336..19677
                     /locus_tag="ACG8PN_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4860664.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19953..21707)
                     /locus_tag="ACG8PN_000485"
     CDS             complement(19953..21707)
                     /locus_tag="ACG8PN_000485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860665.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21718..22554)
                     /locus_tag="ACG8PN_000486"
     CDS             complement(21718..22554)
                     /locus_tag="ACG8PN_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4860666.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22556..23239)
                     /locus_tag="ACG8PN_000487"
     CDS             complement(22556..23239)
                     /locus_tag="ACG8PN_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4860667.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(23398..24792)
                     /gene="uxaC"
                     /locus_tag="ACG8PN_000488"
     CDS             complement(23398..24792)
                     /gene="uxaC"
                     /locus_tag="ACG8PN_000488"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4860668.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(24831..26330)
                     /locus_tag="ACG8PN_000489"
     CDS             complement(24831..26330)
                     /locus_tag="ACG8PN_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4860669.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(26323..27744)
                     /locus_tag="ACG8PN_000490"
     CDS             complement(26323..27744)
                     /locus_tag="ACG8PN_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4860670.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(27804..28796)
                     /locus_tag="ACG8PN_000491"
     CDS             complement(27804..28796)
                     /locus_tag="ACG8PN_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4860671.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            29028..31226
                     /locus_tag="ACG8PN_000492"
     CDS             29028..31226
                     /locus_tag="ACG8PN_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860672.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(31684..32556)
                     /locus_tag="ACG8PN_000493"
     CDS             complement(31684..32556)
                     /locus_tag="ACG8PN_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860673.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT        10591 a         5937 c         6557 g         9615 t
ORIGIN      
        1 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
       61 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      121 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      181 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      241 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      301 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      361 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      421 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      481 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      541 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      601 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
      661 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
      721 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
      781 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
      841 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
      901 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
      961 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1021 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1081 ctttccattc cgtcgttttt ttaccgtttt ttgtttaatt acgatgttgt tctcaccagg
     1141 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1201 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1261 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1321 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1381 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1441 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1501 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1561 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1621 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     1681 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     1741 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     1801 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     1861 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     1921 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     1981 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2041 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2101 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2161 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2221 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2281 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2341 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2401 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2461 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2521 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2581 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     2641 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     2701 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     2761 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     2821 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     2881 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     2941 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3001 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3061 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3121 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3181 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3241 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3301 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3361 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3421 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3481 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3541 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3601 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     3661 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     3721 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     3781 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     3841 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     3901 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     3961 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4021 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4081 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4141 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4201 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4261 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4321 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4381 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4441 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4501 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4561 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4621 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     4681 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     4741 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     4801 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     4861 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     4921 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     4981 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5041 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5101 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5161 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5221 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5281 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5341 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5401 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5461 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5521 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5581 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     5641 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     5701 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     5761 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     5821 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     5881 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     5941 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6001 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6061 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6121 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6181 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6241 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6301 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6361 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6421 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6481 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6541 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6601 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     6661 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     6721 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     6781 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     6841 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     6901 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     6961 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7021 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7081 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7141 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7201 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7261 gtaccgagaa aacagtggag aatcataaaa aaaaaagcca atgtaatgaa ataacttata
     7321 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7381 tcatgacctc tcgataaacg gagttcaaaa gccagtttct cggtattaag ccaaatttat
     7441 gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacagctt
     7501 tttcacgacc tctccactaa atggtgttca atcagctgac cctcggtatt agctcaaaaa
     7561 atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg tcagaacgct
     7621 gatttcacaa cctctcaata aacggtgttc aaaagcttgc atctgcggta ataagaccaa
     7681 ccaaacgaaa aatatgagga tctatttttg cttggctgtt cttattactt gatgcaggac
     7741 agctttttca caacctcttg ttaattatag tttgataagg aaaaaggcaa atcttttgct
     7801 tgatatttct tagggtgagg gatgatccct aattggtaag agatcgtgcc gcctttcagc
     7861 aaatcttgat gggtaaaata taatttcgta tattctctgt ccatccgttt gattttatgg
     7921 ataaattgtt gctgcggccg attaggcgtt gcttcaatcg tcaattcctt tccatttgac
     7981 aaaagcaaaa cagccttgtc aattaatggc ataccgataa catattcacc ggttccaggc
     8041 gtgacaggat aaaatcccag actattgaag atataccaag cggccatact tccattgtcc
     8101 tcatctccag ggtaacccat tggagaatcg tcgaaggtct gagtcatcaa ctgtttaagc
     8161 agtggctgtg ccatttcagg ttgtcctata taactgaaca agtaaggata gtgaaaactt
     8221 ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg ccatctcact catttcgtgg
     8281 atctcaaaac catatcctcc tgggtcaaac gttgggggct gattgcacag ctcaatgagt
     8341 ttttctttat atctctcact gccaccatat tttccgatca gtcctgaaaa atcgtgataa
     8401 acagcaaagc tgctttgcca agcacttcct tctgcataat cttgtcccca acgattagct
     8461 gaaaatggtt ctctgaactt gccatcttta tcttttgaac gcataaatcc tgactcatag
     8521 tcaaagatat tttgatagtt caatgattgc tgctgatatt tcttcgtcat ttcctcttct
     8581 tctagtacct tggcaacttg gctaatacaa aaatcgctat aagcatagtc taatgtgtga
     8641 ttgactgatt cgtggtagtg attaggaaca tagccatatg tcaagtaatc ttctgttcct
     8701 tggcgtccat aatttggatg tttgctttga accgtagcac ctttttccat tgccgttaga
     8761 aattcaggca tcaagtctcg tcggatatct tttacagcag catctgcaat gaccgcatcg
     8821 atcaatgtcc caggcataag tccacgttca tcaggagaca gccattttgg caaatagcca
     8881 ctttctctat agctgttcaa aaaaccttcc agcatttctt cgtatttttc tacagcaatc
     8941 aaagaataaa gaggataaac cgttttatac gtatcccaaa acccattatt tgtatagaga
     9001 acaccaggtt ttatagatcg actagtcgta tcatagtgga ttttttgccc actaggatca
     9061 atttcataaa atgtttgtgg aaataaaaac acccgatata aattatgata aaacgtccgc
     9121 aattgttgtt gatcatggtg tgtgagctgt atacgatcaa aataggactg ccattgttct
     9181 tgactgtgag caaggtaagt ctctttgctt tggttttgtt ctagttccag gttataagct
     9241 gcttgttccg agctgataaa agatgtccca aatcggatag tttgtttttt tgtctgttca
     9301 aaatacaacg ggatcgagtc gctatttttg ttggaaacag ctaactcatc aattgttaaa
     9361 ggatattcaa atctcaatat aaaataaaaa gaaaaatcag gatcttctga accagcaaaa
     9421 tgtgtgattt ttccttccac tgtgtaagga tctataacaa agagctgatg gcgtcccgga
     9481 aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa tagataagat tccgccatac
     9541 atgctaggta tcaatgtaga acagatgcca taacgtaatt gacagatcga cagatgagtc
     9601 gggcaaaata tactttcttc tgggcggtaa gaactttgag catggaaaag ggtgttctcc
     9661 tgtagttttc cattgatcgg cgtaaacagc atatgactga aatcacccat ccaaggactt
     9721 ggttgatggg taagacggta gccttgaaaa acgcggtcat caggatgaaa ccaccagctg
     9781 ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc caaaaggaac gccagtataa
     9841 ggctggcagt tcccattaga gaaatttggc tgattcgctg ttgcatgtct agtatctatt
     9901 tgaccgatat tcattttatt tctcctttgt tgattgtctt ttgatgaatg aaactggaaa
     9961 tttactatcg gatggtcgat cttttgtctc taaccaagtg accaaagcat ctcccgcaag
    10021 cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt ggcgggtcaa caagagctgc
    10081 tgcttggata tcatcaaatc cgataattga aaaatcttca gggatatgat aaccatgctg
    10141 tctcaagaga ttcatggtct ggatagcgac aacatcgttt tcacaaacaa aagcagttac
    10201 ttcatactta gtgatataag aaagcaattc atctaatgtc gcgcttttgt catccagtga
    10261 agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg taaccaaggt aacgctgtcg
    10321 aacggattgt ggatgagtct gcgtaccaaa caaataagcg atttttgaat gtccctgttc
    10381 gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta tcggaaagaa cggtaggaaa
    10441 aggtaactca tagatttttt tatctaatat gacgacaggg aattctttca atgaaagctc
    10501 aaaaagtaag tcaagatgtt gttctgtatc aagagcataa taaatcaatc cgtcaaattc
    10561 attgattagc tcttttgctg ttttattttg tagaaagtca agcgtcgtca tcatgacatc
    10621 aatctgttta tcttgcatca cagggttcaa tcccttgcta aaatcaccaa cagacaaatc
    10681 agcgacatat ggaagaagaa acagaatccg tcgtgttttt gtaggagaag caagaatctg
    10741 ttccggagaa gcggatttaa cgaaactgcc tttcccgcga acgcgataaa taagtcctaa
    10801 ttgctctaat tcagtcaatg cacgtttgga agtgattctg cttactttat aggtacgtga
    10861 aagttctttt tcagttggta gttggctgtc tacaggtaat tcaccagtta aaatagccgt
    10921 ttgcaaatca ttaaatattg tctggtacaa tggttgagtc atataaacac ctcttaatga
    10981 tatatcatat tcaaataata ccataatgga tgaaaaagta aataaaaaga tattttttga
    11041 aagcgtagac ataattggct gtatgaagta taatctttaa agatatatca aatagaaaga
    11101 aggttttatt agtggtttat aaagatatac caaaatcggt gcagaaattt atggaagata
    11161 tcacacaaaa atgtggggat atccatgcgg actgggcaaa gaattttaca gcagcatttg
    11221 ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg aaccaccttt ttattgacgg
    11281 gagacattcc agcgatgtgg ttaagagact ctactgcaca agttcgtcct tatttagtga
    11341 ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg gttagtcaaa aagcaatttt
    11401 tttatatcaa tatcgatcca tatgcgaatg ccttcaacga agaagctaac ggtgctggcc
    11461 accaaacaga tcatacaaaa atgaacgatt ggatctggga aagaaaatat gaaattgatt
    11521 ctttatgcta cccaatacag ttggcctatc ttctttacaa aaatacagga aaaactgatc
    11581 aattcaatca atcttttatt gaaggtatca aaaaaatctt ggacgtgttc aaaacagagc
    11641 aagatcatga acactctcct tatacatttg tacgagacac gacaagaaaa gaagatacgc
    11701 tgatcaatga cgggcgaggt gcaaaaacag cttttacagg aatgacatgg tctggtttcc
    11761 gtcctagcga tgatgcttgc cgttatggat atctggtacc atccaatatg tttgcggtgg
    11821 tcgtgttagg ttatgtggaa gaaatttttc aaacgatact cgatgataaa gaaattgtac
    11881 agcaaacaca gaaattgaag aaagagatcc aagaggggat tcagaatcac ggacgaacgg
    11941 ttaatcaaaa agggcaaacg atttatgcat atgaagtaga tggaatggga aacgccacag
    12001 tcatggatga cagtaatgtg cccaatctga tcgccgcacc ttacttggga tattgttctg
    12061 aacaagagga agattatctg caaacaagaa gaacattgtt gagtaaagaa aatccatatt
    12121 tctatgaagg aaagtacgca aaaggaattg gatcctccca tacgccagaa aaatacgttt
    12181 ggccgattgc attagctatg gaaggaatga caacgtcaga caaatctgaa aaagagcgga
    12241 tattgaattt acttgtagca actgacgcag gaacacacct tatgcacgaa ggattcgatg
    12301 tgaacaatcc tgaaaattat acgagagaat ggttttcttg ggcaaatatg atgttttgcg
    12361 aattagtcat ggattacttt gatattagag tagaaaagta ggaggtatcg taaatgaaaa
    12421 agaaagtata tatcatttct catagccact gggatcgtga atggtacatg gcttacgaac
    12481 agcatcacat gcgactgatt gaattgatag acgatctact agagctattt gaagttgatc
    12541 cgtccttcaa cagttttcat ttggatgggc aaacgattat tttagatgac tatcttcaag
    12601 taaggccaga aaaaagacag gcaatccaac aagcaatcaa tgaaggaaaa ttgagaattg
    12661 gaccatttta tatcttgcaa gatgattttc ttatcagctc ggaatcgaac gtgagaaata
    12721 tgctgattgg catggaagaa agccgtaaat ggggaacccc tgtcatgtta ggctattttc
    12781 ccgatacatt tggaaatatg ggacagacgc ctcaattaat gaaacaagct ggtatttcag
    12841 cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga taatcaagtt ttggaagcag
    12901 aaaactattc ttcccaatac tcagagatgt ggtggaaagg ccctgatcaa acagcaatct
    12961 ttggtctgct atttgccaat tggtatagca acgggaatga gattccagta gaaaaagaag
    13021 cagctcttgc cttttggaag caaaaattag cggatgcaga acaatatgcc tctacgaacc
    13081 acttattgat gatgaatggt gttgatcatc aaccggtaca aaaagatata tcaaaagcaa
    13141 tccatctcgc aaatgaactg tttccagatt acgagtttat tcattctaat tttactgact
    13201 atctggaagc agttcaaaaa gatgtgccag aagacttagg ctcagtagaa ggagaattga
    13261 caagtcaaga aacggatggc tggtatactt tagccaatac agcatctgct cgggtttact
    13321 taaaacaatg gaataccaag gtccagcgac agctagaaaa tattacagaa ccacttgcga
    13381 cgatggctta tgaagttagt ggaaactatc cccatgatca attagattat gcttggaaga
    13441 cattaatgca aaatcatcct cacgacagta tttgcggttg ttcagtcgat tctgtccatc
    13501 gggaaatgat acctcgtttt gaaaaagcag atgaagttgg aaagtatctt gcacaagatt
    13561 ccttagaaca gctgaccgca gcaatcgata cgaccggatt cccaaaagac agctttccat
    13621 ttgtcattgt aaatactgca ggaatggata aaactggtga agcagaaata accatcgaat
    13681 tagaaagaaa aagattcgct gaaggaatac ctgaacaact atatcaagag ttagaaaatt
    13741 tgcctaaacg taagtatcat gtagaaacaa aaagtggggc cactatacca gctattttat
    13801 ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga ccgtttccgt gtgccatata
    13861 tggcaagaat gatcaaagtg actttaccat tagaaaacat gccagcattt tcttgggaaa
    13921 cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa ggaaacaatg attcatcaga
    13981 gtggacgaat catcgaaaac ggccctctac atttgacgat tgaaaaaaat ggtaccatta
    14041 caatggaaga tcgcaaaaac aaacgaaagt tgaataatct gcatattttt gaagatatcg
    14101 gcgatatagg aaatgagtat atctttaaac aacctttctg tgatcagcca atcctatcca
    14161 gtaacaaaga aaactctgaa gtaaaagtat tagtagatac accggaaata gcaaaaataa
    14221 gtattctaca ggaaatggaa atccctgttt ctgcagacga gcgtttagaa aaagaacagc
    14281 agatggttgt ggaatttcgt tatcgaaaag cagaacgatc caaagaaaaa cgaatccttc
    14341 aaataaaaac aatcatgaca atcagaaaag atagtaaaaa aatcgacttt gaaacgactc
    14401 tcgacaatca gatgaaagat catcgcttac gtgtcttgtt tcctacaaag ttacatgtgg
    14461 aacatcacga agcagatagt atttttgaag tggtgaagcg cccaaaccat gtgtctaaaa
    14521 gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt tgtcaatatt catgatgaag
    14581 agtacggtgt aacagtcgga aactttggtt tgaatgaata tgaagtaaca gaagacggac
    14641 aaatcgcagt gacgctactt cgcagtgtgg gagaactagg cgattgggga tattttccaa
    14701 caccagaagc acagtgtcta ggtgagcatc ggttcaatta cagcattgaa ctacatggac
    14761 cagaagaaaa attctctact tatttgcatg catacgcagc acagattcct ttttccactc
    14821 aacaaatcaa acatcatgaa ggaacattga tttcaaaaca acaatattta acgataaaaa
    14881 gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt tagtgataaa gtagtagtaa
    14941 gaggtttcaa tatgtctagt catttagaaa aattggaaat aactaaagac aatggaaaaa
    15001 ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc ggttgtccca ataatccaac
    15061 cttatgagat tcggacaatc ggattcgagg aggaaaacga atgtatggcg gaatagaagc
    15121 aggcggaaca aaatttgtgt gtgcggtttc agatggtgga gaaattttag aaaaattaag
    15181 tatccctacc acaacaccag aagaaacatt ggatcaggta tttgaatttt ttgatcgtta
    15241 tgaattgaag ggaatcggga ttggctcatt cggcccgatt ggtatcgaca aaacaaaaga
    15301 taattacgga tacgttttgg caactcccaa aaaagggtgg gcacaattcg attttcttgg
    15361 cacaatcaag gaacgctaca atattcctgc tgcttggaca acggatgtca atgctgctgc
    15421 atatggggaa ttgaagcaag gagcagcaca aggaaaaaat agttgtattt accttaccgt
    15481 gggaacagga atcggtgcag gtgtcgtgat caatgaagaa attttttcag ggatcgcaca
    15541 tcctgaaatg ggacatattt gggtgaaacg ccatccagat gaccattatg aaggtacttg
    15601 tccttatcat aaagattgtt tagaaggatt agcagcagga ccgtctattg aggcacgcac
    15661 agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt tgggctattc aggcttatta
    15721 tattgctcaa gcactgatca actatacgtt gacgttagca cctgaaaaga ttattttagg
    15781 cggtggggta atgaatcagg atcatctcct tcaaaaaatc cgtcagcaat ttgttgaatt
    15841 gatgggtggt tatatggaaa cacctcaagt aagtgaatat atcgttcgtt ggggactgcc
    15901 gaatgaaagt gggattatcg gtagtttgct gttagcagaa cacgaacatc aaaagtaagc
    15961 agacaaatta cacaaaaaga acagagtctt atcattatct gctgataaga ctctgttctt
    16021 ttttatttct attcccataa tccaggtaaa gctttaccga ctattttttc ttgagtaaat
    16081 ggatggatca tgtgcagttc taatgcatgg agcatcagtc gttcagctgt tctattttgg
    16141 tagagagggt ctccaacgat cggatgtccc ttatgagcta aatgaactct gatctggtgt
    16201 gtacgtccgg tttccaaaac acaatagact tgtgtttgtt cagaaagtac tttgttgaca
    16261 gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta cgaccctttt tctccgatca
    16321 tggcgatctc gtccaatttt attggtgatg gtcatatctt tggcaatttc tccccatacg
    16381 atggcttgat atctccgata gatcattttt tgttccaaca ttcttccaag tatcggcaag
    16441 acaaatggat tttttgcaaa taaaacggca ccgcttgttt ctttgtccaa gcggtggaca
    16501 acgtaaggtc gttgctgttt agtgtcgaga taagcagcta aatgattgag taaggtactg
    16561 ttttctgtcg gttcatttgg atgagtcttc attcctgcag gcttatccac tataatcaaa
    16621 tgctcatctt catataaaac acggatgtgc ttcgcatctc ctaattgaac ttcttgataa
    16681 ttataatctg tttcttcgaa taacaatgtg atctggtcat tgccctttac ttcatgatgg
    16741 aacaaagcag gttgttgatt gatccaaacg tttttacgta tacgcaaaaa gtgtcgaacc
    16801 tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa ttgttgtttc aggatggttc
    16861 ataggtaaag tgatcgtgat tttcacaagt gtactccttt cagtgcaatc gcagactaat
    16921 tgtaccattt ttaaaacata tttaagaaaa aacttacatt tttctgttca ttcttatgtt
    16981 actctacttg cactaacgta aaaaaacata ttgaactaca acttaagcag gaagaaaaca
    17041 ctctttcact atggtaaaat aagacgattg aatcaaatag acaggagaag aagcaaatgg
    17101 attttcaaag cattttagaa aagataaaag ctgctttgat cagtttttgg cgttggatca
    17161 gaccatatct cggacaattc catcgatggc gaaagcgcat ctggaaaaaa tatcatgtga
    17221 acaaattgat tttgctgatt ggtttgatct gtgtcttagt gacaagtatc tacctcttta
    17281 ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg attaagccaa tcgacagtca
    17341 tttatgataa gaaaaacgaa gaagctggca cactgtacgc ccaaaaagga acatatgtag
    17401 aattaaacgc gatttctccc tatatccaag atgcagtaat ctcgacagaa gataggaatt
    17461 tttacgagca tcacgggttt gatatcaaag ggattgcccg tgcagcagtc cgaatggtct
    17521 tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac tcagcagctt gccaaaaatg
    17581 cgtatctgac attagaccaa acgtttgatc gaaaagcgaa ggaattattt ttggcaatcg
    17641 agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat gtatcttaac aatgcctatt
    17701 ttggcaacgg ggtatggggt gtccaagacg cttccagaaa gtattttgga gtagatgcaa
    17761 cgaatgttac gctgtctgag gctgctactt tggcaggaat gctgaaaggt cctggtattt
    17821 ataatccgat cgatcaccca aaaaatgcga atgatcggaa aaacacagta ctaagtgtaa
    17881 tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc agaaagccag acagatatca
    17941 gcgcgtatct taatgacaca tacgataatt cagattctgg ttatcgttat ccttattatt
    18001 ttgatgcagt gatcgatgaa gcagttaacg agtatggtct tgatgaagaa gatgtcatga
    18061 ataaaggcta taaaatctat acagctttag atcaggatta tcaaaagcaa ttagaagcta
    18121 cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga tggtgccatg gtccaatctg
    18181 cttcagtagc tttagatccg aatacaggtg gtgtacaagc actagtagga cgaagaggag
    18241 atcatgtctt tagaggattc agctttgcta cacagatgcg tcgttcacca ggatcaacga
    18301 tcaaaccatt aagcgtatat acaccagcac tggaagctgg atacaagcct tccagtatct
    18361 tgaaagacga accgcaaagt tattacgatg cgcataattt tgacgggaca taccaaggag
    18421 aagtaccgat gtatcaagct gttgcacaaa gtttgaattt gcctactgta tggcttttgc
    18481 atgagatcgg tttacaaaaa ggatatgata aagcagaaga attcggcctg cctttagcaa
    18541 aatctgataa gtattatggc ttagcccttg gtggactgga aaaaggagta tctccattga
    18601 tcatggcggg tgcttacagt gcatttgcca atgatggtca gatgtatacg ccgcatctta
    18661 tcacgaaaat cgttgattcg actggagcgg tgatcgtcga taacacaaac aaaaaaccaa
    18721 aacaggtcat atcaaaggaa acagccgatg aaatgaccag catgcttctt ggtacatttt
    18781 caaatggaac agcggctgct gcgaatcctg caggttatac gatagcaggg aaaacaggaa
    18841 ccacagaaac taatttcgat gccacaaaag caaatgacca atggatgatc ggttacacgc
    18901 cagacgtagt gatttcgaca tggatgggtt acgaagaatc cagcaagctg cactatttgg
    18961 aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc agcggcagaa ggcatccttc
    19021 cgtatacttc aaaaacagga tttagtgtag cagatgccta tgccacagga ggacaagtgg
    19081 tagctgcaga tcaagttgga aacacaggaa attctaatgg agagtctgga aattggcaag
    19141 ataacttgaa tcaatatggt gaacaagcaa aagaagggct gaaaaacttc ggcgatatgg
    19201 tgaaagaagg cgttcaggga attggtgaag cggctaaaga cctctggcgg aaatatcaag
    19261 gagagtagcc cctttgaaaa cgaataaccc catgttacaa tagagagtaa ctactaaaag
    19321 aaaagagggc ataacatgtc aaacatctac gatagcgcga atcaaatcga acgtgagatc
    19381 cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg agaaagtaca agcaaacgaa
    19441 gaagcacaca aactgttcaa agacttccaa gcgatgcaaa tcgaattaca agaaaaacaa
    19501 atgagtggac aagagttcag cgacgaagat gcaatgaaag cacaagaaat ggctgtgaaa
    19561 atccaatcag aagaagtaat caatgacttg atgcaaaaag agcaaggctt cagtacaatc
    19621 atcaatgact tgaaccgtat catcatgaca ccagttcgcg acctttacag cgaataaaac
    19681 tattttatga aacaacagag ggtgtgaaag agacgttcgg ccttgagcga tgacaaaatt
    19741 gcttcttatt gccgataggc tgtctcttga acaccattta ccaagaggtc gtgagcctac
    19801 cgtttaactc tgagttacaa ggaacaattt caaaaaacag cttctcatgt ttgatgacta
    19861 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcagaaa taggagctac
    19921 gacaaaagtc ttgtcatagc tcctattttt tgtcattcat ttttcaagcg gtagaaatag
    19981 cctttttgag ctaacagttt ttcgtaagta cctatttcaa cgatttttcc ttcatcgatc
    20041 acaacgattt tatctgcctc tttgatcgtt gaaagccgat gagccacaat aaaagtagtg
    20101 ggtgtttctg tcaggtaatt cagggcttgc tggatttttt tctctgactg gttatctaaa
    20161 gctgaggttg cttcatctag caaaatgatt tttggctgac gtatcaacgc tcgtgcaatg
    20221 gagatacgtt gcttttggcc accagacagc ttgttgccat gttcgcctac taaggtatcc
    20281 agaccatcag gaagctggtc aatcagtgaa gaaagttgcg cagcttcgat gacttcatct
    20341 aaacgttctt ttgatacgtt tttcaagcca taagtgatgt tctctttgat cgaagcggag
    20401 aacaacaaag tggtttgagg aacaacggct aaataatttc gcacacttcg catatccagt
    20461 tcatccaaag gctgatcatc tagaaaaatt tttcctgaag tcggctggat aaatccaatc
    20521 aataagttaa ggagtgtaga ttttccagag ccagatggcc caacaaaagc aattgtttct
    20581 ccagctttca cctctagaga aaaatgatcg ataatcggtt tgagtgagtc cggataaaga
    20641 aaagaaacat cctcaaaacg aaaatttcca tccaaatgtt ggattttttt cttaccgtga
    20701 taagtttcta catcgccagc aatcaacagt tcgccaatag aagtgataga ttccatgcct
    20761 tttgaaatcg taggaatcag cgtgatcatt ccagaaacgg aattgacgat cgtcgtgaaa
    20821 tacgtctgat acatcacgac atcgccgggt tggatgcgcc cttgtaaagc taatatccca
    20881 gtgaaaatca aacaaatcag ctgaaatatc tgaaaactag cccagctgac tgatccgaaa
    20941 acagattgca ggatatctaa gtggaatcct ttttcataga tttgagtaaa atgcttattt
    21001 aagcgagttg tttcgtgttt ttctaaccca tgagctcgag caatcggtgc taattcgatc
    21061 acttcgatca cttttgcaga agtctcttcc atttcttgcc tgaagtctga gttggactgt
    21121 ctgatccttt tacggaaaaa gagagtaacg aatgcggcga gaggaacagt caataagaaa
    21181 aacatgaaaa caacaggact tttataaagc gtgataccta aagctacgaa taaattgaca
    21241 gcgatattta atccgctgac aaatacttga gaggacaacg tttggatagc ttctacatca
    21301 cggatgatct ttgactggat tcgtccagat tgcaattctt tttgataagg aatagatagt
    21361 tcttggattt tcttgataag ggtcgctctt agtccagctt ctacttgacg gatcgaagtg
    21421 ctatggtatt ttacatgaaa ataattcgtc aatatatttt gaccaatcag acaagccatc
    21481 aaaaaagcgt tgaaataaat ggcctgcaac tgagaagaat ctttcgatga agctgcattg
    21541 attacattgg caatagcgat cggcattaac caagatggaa gatgtttgat aataaaaaag
    21601 agtcctgaca tggcaaaacg ccaataattt ccctgataaa gatgcagcaa tgtctgaaaa
    21661 gcatttgcat gatatttttg ataacttttc tgtattcgtt cctccataat cttctcctta
    21721 acaatcttgg taaataaact gcaagtgttc tattgcttca ggaacataaa tctcaggagt
    21781 agcttcaaga gtggcgtaca ttagaggacg ctcatatttg atataatgca gtaccgggcg
    21841 gaaatctaaa cagcccttgc caacaggtac catgcggatc tttccatttt ccatgatata
    21901 gtctttgaga tgaagcgctg taatataacc atgtaaattg tccaaggcac ctcggatcac
    21961 tttttcctga tcagcatgat tttctttatg catcaaattc gcacaatcta aaatgatttt
    22021 taaatttggt gaagctactt catcgattaa acgtttagct aactgatagt tatagattgg
    22081 atgattgatc cctgcttcaa cagcaacagt tacgccaaaa ttttctgcat gagcgactaa
    22141 ttcaatgata gaatttctgg cgatctgata agcttcttca gtaaaatttt cttttgtgta
    22201 accgacagag acactaccgg tttccgtagc aatcatcttg gcttgatagg cagagcacag
    22261 agcaatttgc tgtttgaaag ttgccaatgc ttgtgcacga acttcttgat ttttgctgga
    22321 taggttgata tagcatccta aaatggaaag ttcgatattg tttttttgga agtattcacc
    22381 aaaataggca gcactcccaa gtgtcatgtc agttaattta agttgttcag gaaatgcttt
    22441 ttgtggtgca aattggatat gggacaagtg aagctgtgat actttttgaa cgatttcttc
    22501 acgatttcga acatctaaat cgtgggcacg gatgcctaaa tttaatggaa acatctcaaa
    22561 ctcctttttg taaataatta gcaagaggca gacgggattc ttttatcaat tcaacaatta
    22621 ttcgggcaac tttatcagcg ccttctggac taaagtgggt attgtctttt agtccttctg
    22681 ggtaattttt acaagtatca ggtataagat gcaggtagaa tttttttgtc tcttcttttg
    22741 agaaggtctg gaacaattct tgtgatcttg agaacacatc taaaagagga atatggtgtt
    22801 tctcagcaaa tgcacgcatc gcttttggat agtctccaag tatatctgga ttcaatgttc
    22861 cgttttctaa gtaatctcgt cgcgtcacag atgtcagtaa gaccatttgg acgttcgctt
    22921 tttttgcgca ttcaaaaaat atctgcagat tttttaaata gctaccgaag ggaagcgtac
    22981 ctctttcttc ttggattttc tggtcgttat gtccaaattg tacaaataag aaatcattcg
    23041 gctgaaactg tgaagctaga cgggctaacc ttccttcctg gataaatgat ttggtgctgc
    23101 gtccattctt tgcttggtta ttgatctgga tctcgggtga aaaataagca gagatttttt
    23161 ctccccaacc tgtctcggga cgcttgtttt catctttttc tgccgcagta gaatcaccgg
    23221 caatatggat agtaggcatg ttatctcttc tttcataaag gtttataggc aatgaagcag
    23281 aacagcaaga gaagaagacg gagcaaattt ggtccgtctt cttctctaaa acgtggataa
    23341 acggattccg ttatttcact atcctgtttt tgattcgtta ctgttactat taatcaatta
    23401 ggcaaaaaag ccaaagtaat ttttagcatt tgtataacta atcgcttgaa ctagacttcc
    23461 taaaagttgt tcatcttcag gtgcttctcc tcgcatgacc atttcgccta aaaattcaca
    23521 aagaacacga cggaaatatt catgtctagt gtaggacagg aaacttcttg aatctgtcag
    23581 catgccgaca aaattcccca aagcactacc atcagcaaat tgtgtcaatt gatcgcgcat
    23641 accagctcga gtatcgttgt accaccatcc agaacctaac tgcagttttc cattctgttc
    23701 tttttggaaa cagcccatca aagctaaaat cgcaggatag tcattcggat tcaatgaata
    23761 aaggatcgtc tttggcaact gatccgtttc ttctgcacgg ttcaataatt tttgcagagg
    23821 aatagtcaaa gaaagatcat tcattccatc atagccggta tcgggcccta atttttccaa
    23881 catttgtgta ttacagttac gataagcatg gatgtgcagc tgcatcgtcc aattatgaga
    23941 atgatagaag tgggtcaaat gtatcagtgt ttctgtacga taagcatcga tttcatctga
    24001 agataatagt ttgttagctc gtgctttttg aaaaatctct tctaatactg tttcatccgc
    24061 ttctttatag cgtaagatgt ctaaagcgtg gtcggaaaca cgacatccat gttgatggaa
    24121 gtattctatc cgctgcccta agacttctat cagttcttga taggtggtga tcgtttggtc
    24181 agctgcttct tccagtagtg tgacccatgc attaaatcct tctttttcaa tattgagtgc
    24241 tttatctgga cggaatgaag gtagaacctt caaatctggt tcttcatgag caagcaactg
    24301 atgatagtgc aagtcgtctg cgggatcatc tgttgtacaa atcacttcta cattcgattt
    24361 ttttgcgatt tcccgacggc gaaaatcagg agctgccaaa atttcatttg cttgtgtcca
    24421 aattttctct gcattttctt catttatcaa caagtcaata ccgaagaaac gcttcaattc
    24481 aagatgtgac caactgtaaa gcggatttcc gataattttt ggtacggttt gacaccaagc
    24541 taaaaatttt tcaaaatcag aagcatttcc tgtgattttt tcttcaggca cgccgcaagc
    24601 tcgcatcaag cgccatttgt aatgatcgcc tcctaaccaa gcttctgtca gattgctgaa
    24661 attgttgttt tcgtaaattt cttttggatc taagtgacag tgataatcaa taatgggcat
    24721 gttttttgcg taggaatggt aaagcttttt cgcccaatcg tcttttaata aaaaatcatc
    24781 agataaaaac ataaatcttc cttctttctt tttatactgt tttatgaaaa tcaaagtgtt
    24841 actccgtttt taaaaatggc aatttctcga acgtcatttt gttcattttt cgtttcttct
    24901 ccactagcta cagcgatgat tttttgaaca aacgattcta agattacttc cattggttgt
    24961 tctaataaga cgcctgcatt aaaatccatc caatgacctt tgcgttcaaa aagagttgta
    25021 ttcgtagata ccttgaccgt tgggacatat gagccaaacg gagtccctct tcctgtggta
    25081 aacaagacca actggcaatc ggatgaagca agagcagagg cagctacaag atcattcccg
    25141 ggagcttgca ataaactaag ccctggtttt ctgatttttt cgccatattg tagcacatca
    25201 acgacaggag agcttcctga tttttgcgtg cagcctaaag acttatcttc taatgtagtg
    25261 atcccacctg ctttattacc aggagatggg ttttcgtaaa ctggttcccc gtaagacaag
    25321 aaatactgtt tgaagtcatt gatcaagtca acgattgagt caaaaacttc ttcattttcc
    25381 gctcgtgcca tcaatacttg ttctgcgcca aacatttctg gcacttctgt taagatcgtg
    25441 ctgcctcctt gagcaatcaa gtagtcagaa aatgcaccaa gtaaaggatt agcagttatt
    25501 cccgatagac catcagaacc gccgcatttc aaaccgacat tcaacttaga gagcgggact
    25561 gatatgcgct ggtcattttc cgccgctgtt aaaagctctt ctaatagagc cacaccttgt
    25621 tcgatctcat cgtaaacatc ttgagccact agaaatttca ctcgttcttc atcatattca
    25681 cctaaaattt ttttgaattc aggaactgta ttgttctcac aacctaatcc aaatactaaa
    25741 actcctccgg cattgggatg atttactgcg tccgctaaaa tttctcgagt attttgatga
    25801 tcttttccaa gctgcgaaca gccataagga tgctttaaaa tcgtaatatg gtcaaactgt
    25861 cccagatctg gatgtttttc cttgaactgt ttgacgatca gttcagcaat gccattcaca
    25921 cacccaacgg taggtactaa aaacaaatca ttacgaatcc ctacttttcc attttttcgc
    25981 aagtaacctt gaaatgttcg gttctcaaaa aggtaatgac ttggatggac atctttttca
    26041 tatcgataat ccaattcacc agaaagattg gtttttacat tatgagtgtg cagccattga
    26101 ccggcagtta cgtctgtcgt cacatgaccg attggatagc catatttgat cacgttgtct
    26161 ccagctttca aatcacccaa tgcaatctta tgaccttgcg ggatatcttc aagagtggtg
    26221 atcgtttgct gatcaatcgt cagttgtgtt tgttttggaa taggggttaa tgcgacgacc
    26281 acactgtctc gtcgatttaa tttcattgtt ggttgcaggg tttcaagcat ggaccatcgt
    26341 tcctttcatt tcttgtaata ctgaccggac acctttttga tcgatctttg cagctatttc
    26401 ttgaactgta tctgtcacat tcggtatttg agacaaatct tctccccaaa gcgcagcgtt
    26461 ttgcaataat cgaggaatag ctgttccatt atcagaccaa gcatcagtaa attcgcttat
    26521 cgtcttttca tcatcatgtg gctttacttg atcgcctcga tacataagaa gtagcgcagc
    26581 tagtgaaaca gtgattaacg gtggtacctg attttgttct tcgatatact tcttgaacac
    26641 tggaagcaga cgggtgctga actttgaaac actatttaat gaaatcgctt ctaatttgtg
    26701 atgggcaaaa ggattcaaga aacgttcttt gatttggtca gcataaatgg ctaattcatc
    26761 tttaggtaaa gaaagcatag gaatcaattc tagattgaat aactgatcaa catatgcgga
    26821 gaataaagga tctttcatta cttcttctac agtttccaat tccgccaaca aagcaagagg
    26881 gaccatcgct gtatgcggtc cattaagcaa atgcacctta cgttcacgat aaggagtcat
    26941 atcatctgtg atgatgacat tcaagccagc ctgtttgaat ggtagttgat tctccaattc
    27001 tttaggccct tcgatcaccc aaagcatgaa tggttctgct ttgaccatca attgatcttc
    27061 atagttatgc cgttgattga aaacacctgc atcttcgcga ggataacctg ggacgatgcg
    27121 atcaactaaa ctacaacaga aaacattttc tttttctaac cactcaacaa aagcaggttc
    27181 taaatcccat ttttctgcgt agcgtaaaat gatttctttt agacgttcgc cgtttctgtc
    27241 aatcagttcg caaggaatga tggtaaagcc agctttgttt aatttgaaac gtcggtataa
    27301 aaaggctgtc agtttgcccg ggaagctctg tgggggagta tctgttaaat tatcagtctc
    27361 cacgaattgg attcctgcct ctgttgtatt ggagacgata taggccaagt cgtcattttc
    27421 tgctagagat agataagcat cccattcatc ataaggattg atcatttgtt ctactacggt
    27481 gataatctcg gaagtatcaa cgatttcgcc attgctgagt ccttgtaaaa tgaccgtata
    27541 taaattatct tgctggttca tcatatttcc aagaccttgt tctaaaggtt gaatgattgc
    27601 aacattacct ttaaacaatt gttggttatt cagctgctga atctgccaat caataaatcc
    27661 acgcataaaa ttgccttcac caaactgaag aacttttaca ggtgcttcgt tttttttcac
    27721 taattctttt gtcagttttt tcatataaac ttcctttctg tgttatgcac acgttaacat
    27781 ttttatataa aattacttgt agtttacaag taattgatcg actcacgaag gatcaacaag
    27841 gtggagagct caattttaga ttgttcgatc gtcctgcctt cgatcaaatc tagtaaaagc
    27901 tttgttcctt cttgacacat ttctttcaaa ggcttacgta cggtggtaag cggcggattg
    27961 agataggaga cgactggaga atcatcgaac ccgatcagag aaatgttctg gggaacacgg
    28021 aggccagcta aatgtacagc tctcaatgca ccaatcgcta tgtcgtcgtt gccgcaaaaa
    28081 accagagtag gcaggtcaga aagtgataat aggtattcca tgttttggag gccgctttct
    28141 aagctgtaat ctcctgttaa aaaataggct ggattgacag ggatgctcgc tttttttaga
    28201 cttttcaata cccctttttc ccgttctttt gtcgaacgaa aatttttctt tccgccaata
    28261 taagcgatct tcgtatgtcc gcatccaata ccgtaatcta ctgcctgcat aacaccggcc
    28321 gcatcatcgg cagtgacgtt gataaccgct ggatcattga tgtgacgatt gacaacaacg
    28381 aaaggaaatt ctttttcttt gagatagtcg atgaaaggct gatcatcatc actttgactc
    28441 ataacgatca gtccatcgta gcgttgcagg ttgatttctg ttaaatcaac aacatcgata
    28501 ctttcaacag ataaactgta atttgtgtct aatactgaat ggatgcctgc aatggaatct
    28561 gctaggaaat ttgatgaagt tcctttatgc aaacttgaga aaaatagtcc gattagatat
    28621 tttttttgat tcactaaacc ttttgcacta tagtttggcg aataattcat ttccttagct
    28681 agttgtcgta ttttttgttt tgtttcattt tttattaaac tgctgttatt caaagcacga
    28741 gaaactgtcg tatgagaaac gccagctgct ttagcgatat ctttgatcgt taccattcca
    28801 tcattttcct tactttttca ctttttgtta acgcattcat catattacga taactacgat
    28861 aagtcaatag atctttgata tgaaaagatt gcacacgtta aaaattactt ggatttttat
    28921 ttttaattat gctattcttg aaagcgggga atgctcattt aattagatga gtatcaattt
    28981 tacagaaaaa tgaaagcctt ttattttaaa aggaggagag gaagaaaatg aagaaaagta
    29041 cagtatttta taaaatattt ttaccgatgt ttttattagg agccgtactt gtcataggat
    29101 ttagtttatt catttaccag aatacctatg aatcgatcga atcgagttat ttaacagaca
    29161 aaaagaattt gctcaggcaa gttaaaacta acgttgaatg gaaaattaga acgattgaat
    29221 attcgttttc tacgtatggt tcgaccaaaa atttctcgga catattcaag catccgctga
    29281 catatacaga ttactccact tattctgaaa tcaggaaaga actaaatttt atagaaacga
    29341 ttgttatgga tgataataat tatgatttag tcagtttggc tggtaaatgg ggagtaatca
    29401 atggatcgtt gagtcaactg accgaagagg aagttgaaaa gtatcaacag aagtacataa
    29461 ataacaaaaa taatttattt tggcaaaaga aaaacaaagg aatcgaaatg gtgatttctt
    29521 tgccaatgtt tgaaaaagaa aaatatgcat tgggaattgc tacgatagag aaacatacaa
    29581 tagaacaagt tgttggtgaa agtggagaag acatactttc tattgaaaac gaagttggca
    29641 acctttttac tactcagaca gtaaaaaata aagagcaacc aactgtatta acggacgtgg
    29701 atctagatta caatcagctg aaagtgataa gagaaaaaga taaaacgtat atagtactga
    29761 aatcagatta taataattgg atttacagat tagaagttga ctctagtgca attggcgcaa
    29821 tgatccgcaa tctgagaatc gggctagttg cagtgtcttt aacactggta ggactcattg
    29881 gtcttctttc ctacttattt tcggacagat ttgcccgtcc gatttctcaa atacaggaac
    29941 gtctgaatct taaatcaaaa ggattcagtg ggaaagaact agatttggtc gctcaatcag
    30001 tcagtcggat cataggagaa aaagaagcgt taagtgcaca cttagttacc caaaagccac
    30061 agctggaaac gctatttgta cttagtttat ttagaaatcg agtagaaagg cgagaactag
    30121 atcagcggtt gcagcaattt ggttatagtt ctcaaaatga ctgctattat acagctttgg
    30181 ttcaaatcga tctgttagaa gatagtcacg gtggggagcg tgatttgcta cttttagcga
    30241 tcaacaacat gatcacggaa attgtgccaa aggaaaatcg aatgctgccg atcgttttga
    30301 atgaagaaat gcaagccact atttatcgaa tggataaaga tgatgcagaa gcaagcaaaa
    30361 aagtcatgga gtattgccga ctcattcaaa aacgtgtaaa agaatacttg aatttaacta
    30421 tcagtatagg catcagtaac cggttcgaaa cgctgaatga aagcaagcaa tcggttgatc
    30481 gggcaaaaga agccctgtat tatcgtgtaa atactggtcc aagttcaatt attttttatc
    30541 aggaaatcgt tcctgctgct catgaaaaaa cacttattag atatccgata gaagaacaaa
    30601 atcgattgtt tgaagccatt cgttctggaa atcaggaagt gaagaattta gtacatgaat
    30661 tgattgatgc attgtttgca caaaataaaa atcctttgag tcgggaagta gtttcggtcc
    30721 gcttgatcaa tgaactcgtt caattaggac agttattggg agtagattca aaaaactttg
    30781 ataacatgaa acaaatttat gtaaaagcac tgaacaatta tcatcccaaa gaactagaaa
    30841 aacttgtttt agaacaatta gttctgccaa tcacagaatg ctctcaagca atcactgatc
    30901 atgaatttaa aaatttgtcg gagaatatta tgcatatcgt ctacacagaa tatgatcgtg
    30961 atctgtcttt agatgtgatc gctgataggt tgcattataa ccctaactat ttaagtaatg
    31021 tatttaaaaa ggaaacagga gaaaaatttg gtgattttgt tcaaaatcat cgtttagagg
    31081 tagcaaaaaa atggttgaaa gagactaacc tctccgtaaa agagattgca gaaagattgc
    31141 aatatcgaaa tccgcaaaat tttattcgtt tctttaaaaa gaaggaatcg attacgccgg
    31201 gagaatatcg aaaaaaatat ttgtgaatca agaggctgtt tttcttgtgg actgattcaa
    31261 gcaataaaag aagttagaat aaccctagta tttgtattta tggatagtta aatttcaagg
    31321 ctaagcacgc aaaaagaact gtgacaaaag tcttgtcaca gttctttttt acgaataaat
    31381 ggcgttcaag agacaggcta tcgacaataa ggcataattt tgtgaaatat gggaagctat
    31441 ttttcaaaat cgcttcttat tgctcctgtg ggttactcgt cgcaatgttt gtgagagttt
    31501 ttatcatcgc tcaaggctga acgtctcttt cacaacctct gatacacggt gttccaggag
    31561 tagattcttc gaaaaataag ccgaaatttc acaaaaattt aagaagcaat tttcgtaaat
    31621 gccttcttat ttcttgaagc tgaccacttc tgacacaact accattttgc gttcagtaca
    31681 taatcaccct ttgacagaac ctacgagtgc tcctttgaca aaatatttct gaacaaaagg
    31741 ataaacaatc agcataggca ctgttgcaat aacgataaca gccattttaa ctgtttgtgc
    31801 tggtggaatc acatcaacgg cactactttc tgcttgcatc ccacttgata caatgacgat
    31861 ttggcgtaaa agaatctgta tcggccattt agatgaatcg ttgatataca aaatagcagt
    31921 catataagtg ttccagtaag taacagcata aaaaagcgag atagtagcaa tagacggtaa
    31981 cgctaatggc agcataattt tgaaaaatac accaaaatct gtgcaaccat ccatttttgc
    32041 tgattcttcc aaactatccg gcaaggcttg gaaaaagttt ctcataatga tcatattcat
    32101 tgcattaaca gccacaggca ggatcatggc ccagtaagaa ttgaccagcc ctagagaacg
    32161 aacaactaag aacgttggaa tcatcccccc gctgaacaac atagaaaata caacaagaaa
    32221 gttgacgaaa cctcgaccaa acaaatactt tctggaaaga ccgtaggcca tcagtgaagt
    32281 tgcacacata ctgacgatcg ttccgacacc tgtaacacca atcgaaacag caagtgctct
    32341 aaaaatggtc ggcgtagaaa gaatgtagcg atacgcgtct aaagaaaagg tagtaggaaa
    32401 aaggataaac tttttcgcga cgacttcttg tgtagaagca aaagatgtag cgatgatatt
    32461 taaaaaagga acgatacaga tcaaagctag caaaattaga aacgtattgt tgaaaagtgt
    32521 aaaacttctt tctcctatag agactttctt tttcatatag ataggctcct cctcaaaata
    32581 aattaaatgt ttctgtttct atatgttcaa tttatcatcg gtgaaagatt acgtatataa
    32641 tcatttccta tgctaagtgt cagatagaaa aaaatagaag gtttgctatg cgtagtttat
//
LOCUS       DAZUKA010000013        31451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000013 DAZUKA010000000
VERSION     DAZUKA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.013"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8PN_000494"
     CDS             212..604
                     /locus_tag="ACG8PN_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4860674.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8PN_000495"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8PN_000495"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4860675.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8PN_000496"
     CDS             2698..2943
                     /locus_tag="ACG8PN_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860676.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8PN_000497"
     CDS             3165..4775
                     /locus_tag="ACG8PN_000497"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4860677.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8PN_000498"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8PN_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860678.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8PN_000499"
     CDS             5067..5936
                     /locus_tag="ACG8PN_000499"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4860679.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8PN_000500"
     CDS             6101..7372
                     /locus_tag="ACG8PN_000500"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4860680.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8PN_000501"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8PN_000501"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4860681.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8PN_000502"
     CDS             8763..9026
                     /locus_tag="ACG8PN_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4860682.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8PN_000503"
     CDS             9471..9920
                     /locus_tag="ACG8PN_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4860683.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8PN_000504"
     CDS             9977..11395
                     /locus_tag="ACG8PN_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4860684.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8PN_000505"
     CDS             11395..12255
                     /locus_tag="ACG8PN_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4860685.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8PN_000506"
     CDS             12429..13202
                     /locus_tag="ACG8PN_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4860686.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8PN_000507"
     CDS             13349..13714
                     /locus_tag="ACG8PN_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4860687.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8PN_000508"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8PN_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4860688.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8PN_000509"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8PN_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860689.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8PN_000510"
     CDS             15560..16216
                     /locus_tag="ACG8PN_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4860690.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8PN_000511"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8PN_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4860691.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8PN_000512"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8PN_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4860692.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8PN_000513"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8PN_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4860693.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8PN_000514"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8PN_000514"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4860694.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8PN_000515"
     CDS             21888..22202
                     /locus_tag="ACG8PN_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4860695.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8PN_000516"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8PN_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4860696.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8PN_000517"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8PN_000517"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4860697.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8PN_000518"
     CDS             23546..23875
                     /locus_tag="ACG8PN_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4860698.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8PN_000519"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8PN_000519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4860699.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8PN_000520"
     CDS             24863..25687
                     /locus_tag="ACG8PN_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4860700.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8PN_000521"
     CDS             25680..26027
                     /locus_tag="ACG8PN_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4860701.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8PN_000522"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8PN_000522"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4860702.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8PN_000523"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8PN_000523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4860703.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8PN_000524"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8PN_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4860704.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8PN_000525"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8PN_000525"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4860705.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10111 a         5634 c         6633 g         9073 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt g
//
LOCUS       DAZUKA010000014        30923 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000014 DAZUKA010000000
VERSION     DAZUKA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.014"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8PN_000526"
     CDS             complement(296..1024)
                     /locus_tag="ACG8PN_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860706.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8PN_000527"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8PN_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4860707.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8PN_000528"
     CDS             2805..3743
                     /locus_tag="ACG8PN_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860708.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8PN_000529"
     CDS             3755..4666
                     /locus_tag="ACG8PN_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860709.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8PN_000530"
     CDS             4690..6162
                     /locus_tag="ACG8PN_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4860710.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8PN_000531"
     CDS             6213..6824
                     /locus_tag="ACG8PN_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4860711.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8PN_000532"
     CDS             6854..8767
                     /locus_tag="ACG8PN_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS4860712.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8PN_000533"
     CDS             8895..11876
                     /locus_tag="ACG8PN_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS4860713.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8PN_000534"
     CDS             11873..13534
                     /locus_tag="ACG8PN_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS4860714.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8PN_000535"
     CDS             13574..14506
                     /locus_tag="ACG8PN_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4860715.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACG8PN_000536"
     CDS             complement(14898..16445)
                     /locus_tag="ACG8PN_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4860716.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG8PN_000537"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG8PN_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4860717.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACG8PN_000538"
     CDS             complement(16890..17084)
                     /locus_tag="ACG8PN_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860718.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACG8PN_000539"
     CDS             complement(17310..18512)
                     /locus_tag="ACG8PN_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4860719.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACG8PN_000540"
     CDS             complement(18515..18709)
                     /locus_tag="ACG8PN_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4860720.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACG8PN_000541"
     CDS             complement(18756..19604)
                     /locus_tag="ACG8PN_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS4860721.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACG8PN_000542"
     CDS             complement(19809..20159)
                     /locus_tag="ACG8PN_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860722.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACG8PN_000543"
     CDS             20337..21200
                     /locus_tag="ACG8PN_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4860723.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACG8PN_000544"
     CDS             21824..22816
                     /locus_tag="ACG8PN_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860724.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACG8PN_000545"
     CDS             22832..23716
                     /locus_tag="ACG8PN_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860725.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACG8PN_000546"
     CDS             23765..25372
                     /locus_tag="ACG8PN_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4860726.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACG8PN_000547"
     CDS             25373..26857
                     /locus_tag="ACG8PN_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4860727.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACG8PN_000548"
     CDS             27034..28026
                     /locus_tag="ACG8PN_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4860728.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACG8PN_000549"
     CDS             28078..28947
                     /locus_tag="ACG8PN_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4860729.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..30896)
                     /gene="guaA"
                     /locus_tag="ACG8PN_000550"
     CDS             complement(29367..30896)
                     /gene="guaA"
                     /locus_tag="ACG8PN_000550"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4860730.1"
                     /translation="MIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPKG
                     IILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKA
                     ELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMY
                     GIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGL
                     SGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKE
                     RFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTE
                     TAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGL
                     GIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTY
                     DYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEW
                     E"
BASE COUNT         9693 a         5150 c         6241 g         9839 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataattttt
    30901 ggctcttcgt caaatcgtgt tgg
//
LOCUS       DAZUKA010000015        30518 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000015 DAZUKA010000000
VERSION     DAZUKA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.015"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..203)
                     /locus_tag="ACG8PN_000551"
     CDS             complement(<1..203)
                     /locus_tag="ACG8PN_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860731.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRI"
     gene            complement(191..820)
                     /gene="pyrE"
                     /locus_tag="ACG8PN_000552"
     CDS             complement(191..820)
                     /gene="pyrE"
                     /locus_tag="ACG8PN_000552"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4860732.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(827..1543)
                     /gene="pyrF"
                     /locus_tag="ACG8PN_000553"
     CDS             complement(827..1543)
                     /gene="pyrF"
                     /locus_tag="ACG8PN_000553"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4860733.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1540..2472)
                     /locus_tag="ACG8PN_000554"
     CDS             complement(1540..2472)
                     /locus_tag="ACG8PN_000554"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4860734.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2469..3245)
                     /locus_tag="ACG8PN_000555"
     CDS             complement(2469..3245)
                     /locus_tag="ACG8PN_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4860735.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3299..6484)
                     /gene="carB"
                     /locus_tag="ACG8PN_000556"
     CDS             complement(3299..6484)
                     /gene="carB"
                     /locus_tag="ACG8PN_000556"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4860736.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6489..7565)
                     /locus_tag="ACG8PN_000557"
     CDS             complement(6489..7565)
                     /locus_tag="ACG8PN_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4860737.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7570..8850)
                     /locus_tag="ACG8PN_000558"
     CDS             complement(7570..8850)
                     /locus_tag="ACG8PN_000558"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4860738.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8869..9795)
                     /locus_tag="ACG8PN_000559"
     CDS             complement(8869..9795)
                     /locus_tag="ACG8PN_000559"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4860739.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9808..11091)
                     /locus_tag="ACG8PN_000560"
     CDS             complement(9808..11091)
                     /locus_tag="ACG8PN_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4860740.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11105..11644)
                     /gene="pyrR"
                     /locus_tag="ACG8PN_000561"
     CDS             complement(11105..11644)
                     /gene="pyrR"
                     /locus_tag="ACG8PN_000561"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4860741.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11879..12790)
                     /locus_tag="ACG8PN_000562"
     CDS             complement(11879..12790)
                     /locus_tag="ACG8PN_000562"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4860742.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12787..13251)
                     /gene="lspA"
                     /locus_tag="ACG8PN_000563"
     CDS             complement(12787..13251)
                     /gene="lspA"
                     /locus_tag="ACG8PN_000563"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4860743.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13256..13954)
                     /locus_tag="ACG8PN_000564"
     CDS             complement(13256..13954)
                     /locus_tag="ACG8PN_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4860744.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13941..14327)
                     /locus_tag="ACG8PN_000565"
     CDS             complement(13941..14327)
                     /locus_tag="ACG8PN_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860745.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14474..16144)
                     /locus_tag="ACG8PN_000566"
     CDS             complement(14474..16144)
                     /locus_tag="ACG8PN_000566"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4860746.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16327..16560
                     /locus_tag="ACG8PN_000567"
     CDS             16327..16560
                     /locus_tag="ACG8PN_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4860747.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16659..17090)
                     /locus_tag="ACG8PN_000568"
     CDS             complement(16659..17090)
                     /locus_tag="ACG8PN_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4860748.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17169..17582
                     /locus_tag="ACG8PN_000569"
     CDS             17169..17582
                     /locus_tag="ACG8PN_000569"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4860749.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17620..18768)
                     /locus_tag="ACG8PN_000570"
     CDS             complement(17620..18768)
                     /locus_tag="ACG8PN_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4860750.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18890..19486)
                     /locus_tag="ACG8PN_000571"
     CDS             complement(18890..19486)
                     /locus_tag="ACG8PN_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4860751.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19686..22328)
                     /gene="alaS"
                     /locus_tag="ACG8PN_000572"
     CDS             complement(19686..22328)
                     /gene="alaS"
                     /locus_tag="ACG8PN_000572"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4860752.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22716..23147
                     /locus_tag="ACG8PN_000573"
     CDS             22716..23147
                     /locus_tag="ACG8PN_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4860753.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23192..24541)
                     /locus_tag="ACG8PN_000574"
     CDS             complement(23192..24541)
                     /locus_tag="ACG8PN_000574"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4860754.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24611..25774)
                     /locus_tag="ACG8PN_000575"
     CDS             complement(24611..25774)
                     /locus_tag="ACG8PN_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4860755.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25903..26226
                     /locus_tag="ACG8PN_000576"
     CDS             25903..26226
                     /locus_tag="ACG8PN_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860756.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26409..27758)
                     /locus_tag="ACG8PN_000577"
     CDS             complement(26409..27758)
                     /locus_tag="ACG8PN_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4860757.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27827..28780)
                     /locus_tag="ACG8PN_000578"
     CDS             complement(27827..28780)
                     /locus_tag="ACG8PN_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4860758.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28882..30072)
                     /locus_tag="ACG8PN_000579"
     CDS             complement(28882..30072)
                     /locus_tag="ACG8PN_000579"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4860759.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30272..>30518)
                     /locus_tag="ACG8PN_000580"
     CDS             complement(30272..>30518)
                     /locus_tag="ACG8PN_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860760.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         8576 a         6693 c         5352 g         9897 t
ORIGIN      
        1 attattctct tctttctgct aaccagtctt agaaaaatga attttaaaaa aatcaaaaac
       61 tgatcacgat agaagtttct tttcatacct gatgaaaaga tgcttctcgc aatcagtttt
      121 tggatttttg ctattccaat tgatgattct ggagacacaa atcggatttc tctatcttca
      181 tcaagcgcgc ttaaatctgc caattttcag gatctttttt ccattctttt agcagagtta
      241 attcttgctc atcgatataa cgatcttcta aagctgcttc aatcaataca gaataattgg
      301 tcaacgtcat aagagggatt tcggcttttt cgaaatttgc ttttcctttt ggcaactcat
      361 acgtaaagat agccgcaaca ccaaggatat ccgcaccttc tctttttgct gcttcagctg
      421 cttccaagac acttccgcct gtagaaatca aatcctcgat cacaaccatc ttctgacctt
      481 caaatatacg tccttcgatt tggttacctt tcccatgatc tttcgcctta cttcgaatat
      541 agaccattgg caaatccagt atttccgcta cccatgcggc atgagggatt cctgctgttg
      601 cagttccagc aatcacttgt acttcagggt aagcttcttt gattttgctt gctaatcctt
      661 ttgcgatctc ttttcggact tttggatagc tcatcgtgat tcggttatca cagtaaattg
      721 ggcttttgat tccacttgcc catgtaaaag gattacttgg atttaaaaat actgcttcaa
      781 tttctaataa gtcttttgcg atactttgtt caacattcat tttaggttag ctccattctg
      841 tttgaatcgt ttgatatgct ttataaggat tagttgactg agtgattggt cgacctataa
      901 cgataaaagt tgagccgatc ttttttgcct gagaaggtgt gactactctt gtctgatctc
      961 ctgcgactgt tccttctggt cttatacccg gagttaagca aacaaattcg tctcctgcca
     1021 tttctttgat cgcttctact tcccaagcag aagaaaccac accatctaat cctgcttttt
     1081 tggcacagct agcataatgg atcacacttt tttctaaaga aacttcgatt agttgatcgg
     1141 catgcatttc ttcttcactt gtagaagtaa gctgagtgat agctagaagt agcggacgtt
     1201 ttccaccttc tggagtcccc tcttctaatc ctcgcattgc cgcctccatc atacgaatac
     1261 cgccagcggc atgaacacag gtgagatcaa caccaagctt tgccaatccc cgcattgctt
     1321 tttcgaccgt attcggaata tcgtgaagtt tcagatctaa aaatacatta tgaccagcct
     1381 cttttagata acgtacgatt tcaggtcctt cttggtagaa caattccatt ccgattttca
     1441 caaacaattt ttcttctttc gggaaaaatc gaagaaagtc ttctacctct tgcctagttg
     1501 aaaaatctaa tgcaataatt ggtcgttggt ctagttgatt catcgttcac gttcctctct
     1561 cacttctttg attaatcttt ccagcgattc gatgcctagt tcgctcattc gcacagggag
     1621 ctctttaatt agtttaggac agatatacgg atcggtgaaa ttcgcggtcc ctatagctac
     1681 agcacttgct ccagccataa acatctcaag aacatcatcc actgtctgca cgccgcccat
     1741 cccaatgatt ggaaggctgg atatacttgc tacttggtga atcaaacgaa tagcgacagg
     1801 cttgattgct ggtccggaga gtccacctgt ttgattggct aaaatcggtt ttcttgtctt
     1861 aaggtcaatc cgcataccaa gaagagtatt gatcatcgta aacccatctg cccctccagc
     1921 ttcgattgcc tgagcgattg gaacgatgtc tgttacattt ggggacaatt tgacatagac
     1981 aggaacagaa gaaactttct ttactgcttg tgttaattga aaagcaacat cagaatctgt
     2041 tccaaaagca attccgccat gtttgacatt tgggcaagaa atgttcagct cgattgcttt
     2101 cacatttggt gcttcgccaa ttttgctaca aacttctaca tagtcttctt cacaagcacc
     2161 agcaacattt gcgatgatgg gtaactcatt gtactcctct aattctggaa gaagtttatg
     2221 cataacaacg tctagaccag gattttgcaa accaattgca ttcaacatgc cagatggtgt
     2281 ttccgccact cttggcgtgg gattgccaaa gcgggcattt ggagtcgttg ccttgaccat
     2341 gatcgatcca agctgattca aatcatagta ttttccgtat tctttgccaa aaccgaaaca
     2401 gccgcttgcc ggcatgatag gattttttaa ttctaggcca ggaagtttga ccgctaaagg
     2461 attctcattc atataattac ctctcctatt ttaaaaatcg gtccttcgtc acaaactttc
     2521 acactttttg ttcctgtttc atcttccggc gtatgacaaa cgcaagcata gcaagcccct
     2581 atcccacatg ccatgcggga ttctaatgat aactggacat tttgatgcga agaaaacagc
     2641 tgttccactg tcttcaataa cccgtttgaa ccacatgcaa aaacagctgt aggttccgtc
     2701 ttcgttccta acaataaatt accgacattt ccgtgcatac cgtatgttcc atcgtcagta
     2761 gcgattcttg tttctcctag acgattaaat tctttttcat aataaacgac ttcatgtgtg
     2821 gcaaaaccta aaaaatgcgt tggcttaatt cctttttcga ctaactgttt tgataactca
     2881 tatagaggag ggacaccgat tcctccaccg attataaata cttcgtctcc tgtatgaaga
     2941 ttggacaaat caaaaccatt gcctaaagga cccatgacat ctaaatggtc tccggcagaa
     3001 agagtagaga agactctagt cccctcacct tccacccgat aaatgatacg gcaagtattt
     3061 gtttcgtggt cgatatgatt caagctaatc ggtcggcgta atagcaagtc tgcacgcggc
     3121 acgagaatat gcaagaactg tcctggttgc tgcatttctt ttactagatc acctttcaat
     3181 gtcatttcaa aaatctttgg tgccagctgt ttttgctgaa cgatttccat catttcttgt
     3241 ttcatctgtc ccctcctgca acggtttttc gtctttttgt cttaacatgt gctcgccgtt
     3301 aaatagggtt agttgaaaaa gcacgggatt ccatcactcg aacgatcgca tctgctgtgt
     3361 ctaaagaagt aaacaatgga ataccatgtt caactgcttc tcttcggatg atgaaaccgt
     3421 ctttagaagc atttccccga tctttatcaa tcgtattgac aaccacttgt gttttaccat
     3481 ctcttattaa gtcgactaca tttttttctg ctgcttcctc agaaattttc gctactgttt
     3541 caacgaccag accatttttt tcgaaataag cagctgtatg tttcgttcct atcagactat
     3601 agccaatttc tgtaaaacgt ttagctaatt ctagtgcttc ttcttttgtt tcgtccgcaa
     3661 tcgtaaataa tacggctcca tattcaggca agtgtaatcc agaagcttcg aatgccttgt
     3721 acaaagcttt gtctaattgt ctgtcagatc ccattacttc tccagtcgat ttcatttcag
     3781 gccctaagta agtatcaact ttctgcaatt tcgtaaaaga aaagacaggt gctttaacat
     3841 gaacttcttt tgtttctgga taaagtccgt tttcataacc aagtgtcttt agtttctctc
     3901 ccaaaatagc tttcgtcgca atttgtgcca ttggaattcc tgtcacctta cttaagaatg
     3961 gaacagtccg acttgctcga ggattgactt cgatcacata aacctcattt tcatggatga
     4021 caaactggat attcatcatc ccgacacaat tcaaacctaa tgctaagcgg atcgtgtaat
     4081 cttcgatcgt tttcttgatc gattcagata atgtttgagg tggatacaca gccattgaat
     4141 cccctgaatg gacaccagca cgctcaatat gctccatgat ccctgggatg agtactgttt
     4201 caccgtcaca aatcgcatcc acttcgcatt caccaccaag caagtaacgg tccactagta
     4261 ctggatgttc tggagatgct ttcacagcat gttccatgta atcttctaaa tctcgctggt
     4321 tttcaacgat ttccatcgca cgtccgccta atacatagct tggacgaaca agaactggat
     4381 aaccaattct cgaagcgata accacggctt cttctttact tgtagcagta tcccctaaag
     4441 gttgaggaat atccaactct ttcagcgctt gctcgaataa atcacgattt tccgcacgat
     4501 ccaagtcttc gatcgttgtc cctaagattt ttacaccagc agcagtcaaa ggttcggcta
     4561 agttgattgc tgtctgacca ccaaattgaa cgattactcc tatcggctgt tctagctcga
     4621 tcacattcat cacatcttcg aaggtcaatg gctcaaaata taatttatcc gaaatagaaa
     4681 agtcagtcga aactgtttca ggattgctgt tcatgatgat tgcttcgtat cctgcctgct
     4741 ggattgcttt gacagaatgc acagtcgcat agtcaaattc aaccccttgt ccgatacgga
     4801 taggaccaga tcccagtact agaattgagg gcttttctga tcgtgaactt tcattttcat
     4861 attcatagga actataaaaa taaggtgttt ttgattcgaa ttccgctgca caagtgtcaa
     4921 ccatcttata tacaggaatc aaatcatttt cttttctcat ctggcgaacg gcttcttcgt
     4981 ttatttccca cagctccgcc acttttttgt ctgtaaaacc gttttgtttc gctgttttca
     5041 atatcttgtc atccaacggc tgctgtttca actgctgttc gatttctacg atatgcaaca
     5101 gtttatctaa aaagaataaa tcgatttttg tcaaatccgc taattcttct atggtgtacc
     5161 ctcttctaat agcttcagct acataaaaca ggcgatcatc ttgtgctcga accacttttt
     5221 ggattaggtc atcgtccgtg atgtctgcca actcaggaag ttcgatatgg tgtgtaccga
     5281 tttctaaaga acgcacagct ttcaataaag actcttcgat attccgaccg atagccatca
     5341 cttcaccagt tgctttcatc tgcgtgccta aaagccgttc tccgttttca aacttatcaa
     5401 acggccagcg aggaatttta gctacaacat aatctaatgc aggttcaaat tctgcaaaag
     5461 tcgttcctgt aacaggattt ttcatttcgt ctaatgttaa tccaagcgct attttagcag
     5521 ctagcttagc gatcggatag ccagtcgcct tacttgctaa cgcagatgaa cgagaaacac
     5581 gcggattgac ttcaatcaca taataattaa aactatgtgg atcaagagcg agttggacat
     5641 tacagcctcc ctcgatctta agtgctcgaa taatcgaaag agaagcatca cgaagcattt
     5701 gatattcatg gtcagacaat gtttgactag gggcaaatac gatagaatca cctgtatgga
     5761 tacccaccgg gtcaaaattt tccatattgc agacaacgat tgcattatca gccgaatctc
     5821 tcatcacttc atattcaatt tctttaaaac ctgcaatact tcgttcaatc aaacattggg
     5881 tagcggggga aagacttaat ccattttctg atacgatacg taattcttct tcattttcac
     5941 acatacctcc accagttccg cctaaagtaa aggctggacg aacgatgaca ggatagccga
     6001 ttttatttgc aaaatctaca gcttcttcta ctgtattgac gatttcagac tcggggattg
     6061 gctgattcaa ttctttcatc aactgtttga atagatcacg atcttctgct tgatcgatcg
     6121 cagaaagctt tgttcctaga agttctatgt tcaattcttc taaaatacct gatgctgcca
     6181 gctccattgc catattgagg cctgtctgtc cgcctaaagt tggaaggaga gcatctggac
     6241 gctcttttcg aagaatcctt gatacaaatt caagtgtgat cggttcgata tatacttgat
     6301 ctgccacttc tttatccgtc atgatcgtcg caggattaga atttactaaa accacttcat
     6361 atccttcttc ttttaaggca aggcaggctt gtgttcctgc gtagtcaaat tctgcggctt
     6421 gaccaatgat gatcggacca gatccaatca ccataatttt cttgatatct gtacgtttcg
     6481 gcatctgttt actgctcctt tcctgcgtcc atcatttcca taaattcatc aaataaatgt
     6541 agtgcatcat gcggaccagg ggcagcatct ggatgaaact gcacactgaa agcgggataa
     6601 tctctgtgtt tgactccttc tactgttccg tcatttactt ctacatgagt tacgatgagt
     6661 ttttctggat caatcgtggc ttcatcgact gcatagccat gattttgaga agtaaaatca
     6721 attcttccgg tcgctacttc tcgtaccggg tgatttaatc ctcgatgtcc gaatttcatt
     6781 ttataggtat ctgcaccgtt ggctaaagca aataactggt gaccgagaca aataccaaaa
     6841 atcggtactt ttccttggat ttttttgatc atttcgattg cttctggcac atcttttgga
     6901 tcaccaggtc cattcgtcag catgactccg tctggtgata gcgctaaaat cgtctcggca
     6961 tctgtgttat agggaagtac tgtgaggtta cagtttcttt tactcaattc tcgtaaaata
     7021 ctatgtttca agccgaagtc aatgacaatg actttccgac ctgttcctgg acttggataa
     7081 ggtttgttag ttgagacttg ctgcacttga ttggttgcta gaacggttgc tttcagctga
     7141 tcaaatgcat gttcgaagga atctccagca tcaactatac tagctttcat cgttccgact
     7201 tcacggattt tgcgagtaag ggcgcgcgta tcgatacctg atattccagg aattcctttt
     7261 tgtttcaaaa atgaatctag cgtcatttgg tttcgccagt ttgaagctac acgtgcatgt
     7321 tcttttacta ctactccttt gcatgttgga gaaatcgatt cataatcatc tctatttacc
     7381 ccataattac ccaccatagg ataggtaaat gtaatgattt gaccgttaaa actttgatca
     7441 gtgatagttt cttgataacc agtcatactt gttgtaaaaa cgatttctcc gaagacacta
     7501 gcatctgcac caaaagcctt tccttcgaaa actgttccat cttctaatat aagcaaacgt
     7561 tccatacttt tattctccct tctgccacac aagttttccg ttgacaaaag tcatttgtgt
     7621 ttcgcctttt actttccagc cgataaaagg tgtattctcc ccttttgata aaaagtcttc
     7681 tttatcgatc gtgcaagttt gtgtaatatc aaaaactgct acatcagcgg gggcaccaac
     7741 cgttagcgtt cctgcattca aaccaaaaat ctcagccggt ttaactgcca accaattgat
     7801 cacttgttcc aacgtgaatc gaccagtttc tacaaaatga gtatagatca gttgaaacgc
     7861 tgtttcgctt cctacgatac caaatggtga tttcatgaaa ctttggcttt tttcttctaa
     7921 tccatggggg gcatggtctg tagcgatgca atcgatcgta ccgtcaagca agccttcgat
     7981 caaagcttct ctgtcctccc gtcctcttag cggcgggttc attttccaaa agccgaaatc
     8041 ttcgggaata tcttcgtcga tcaaaataag atgatggggt gaaacttctg ctgttacgtg
     8101 gattcctgct ttttttgcgt ctctgattac acggacactt tctttagtag aaacatggca
     8161 aacatggtaa tgaacaccag ttgcttcagc tagcaacaga tctctagcaa tttgagaaga
     8221 ctcggttacg cttaagatcc cgggaagacc aagttcttct gccttcttgc cagcgtgcat
     8281 aacgccgcca aataataaag attcatcttc tgtatgggct actagggctt tattgttttt
     8341 tgcagcttct ttcatagcca gatacatcgt cccagctgtt tggacaccta caccgtcatt
     8401 tgtgaaagca aaagcacctt cttcaatcag tgcttcttga tcaactaatt tctcacttcg
     8461 cagattttct gtgattggcg catactgcaa taccttcaca actgcatctt ttctaatgat
     8521 gtcatatact tgtctcagct tttctgccgt atcaggtaca ggatttaaat tcggcatcgc
     8581 gcatacagta gtgaaaccgc cacgagcagc tgagcgagtc ccagcttcga tcgtctcctt
     8641 gtatgtaaat cctggctctc tcaaatgaac atggacgtcc actaaaccag gtgtgatcaa
     8701 ttgtcccttt gcatcaaaca cttcatcaaa ttcggcttct gagaatcctg taccgattgc
     8761 tttgattcgc ccatcttcta tccaaatttc tgctggtgtc gtcatatttt ttcgtgtatt
     8821 gatctgaccg tttttaatga gtgttttcat tttttcatcc tcctataatt acgcttttcc
     8881 atttagcact gcttctaaga ttgccattct gacgaatact ccattgctca tttgctgaac
     8941 gatccgcgac tgtaatcctt ccactaatga atcagctaat tctacatcac ggttaactgg
     9001 tgctgggtgc ataatgattg catgtttttg caacttcttc gcacgctcaa ctgttaaccc
     9061 atgttcctga tggtagcctt cttttgagaa actttctttt ccatcatggc gttcgtgttg
     9121 cacccgaagc atcatcatga catcaacttg atctagtagt tcatccaatg gcatataatg
     9181 tccgtacact tcgaattcat cgtcgtacca ttctctagga cctgagaaga atacttgtgc
     9241 gcctagacgt tttaacatct gcatattgga tttagctacg cgagagtgag tgatatcacc
     9301 tacgatggca actttcagac cttcaaaatg accaaattct tcataaatgg tcatcaaatc
     9361 aagcaagcat tgggttggat gctgtccgct tccgtctcct ccattgataa tcgaacactg
     9421 gattgtttta ctttgtatca gttcgtcata atagttctca tcaccatgac ggataaccgc
     9481 tacgtctacg cctaatgctg acatcgtcag tactgtatcg tatagtgttt cccctttttg
     9541 gacagagctt gtgcttgctt caaattcaat cacttctagc cctagttttt tctccgctac
     9601 atcgaagctt ttatgtgtgc gagtactatt ttcgaaaaag aggtttgtag caaagtactg
     9661 gcgcttttgc ggagtccaat tagcaccttg cttgaattcc tgccctctgc ggatcaagcc
     9721 cataacttct tgatcagtaa gtgcctcaac ggtcaataaa tgctttaagc tgatacgttc
     9781 tgatttgatg atcataatgt ttcctcctta gttttcttcg cttcgtgaag tttctggtaa
     9841 gataagattt aaaatgatgc caagtacagt tgctaatgcc attgccgaca atgtaaatgt
     9901 gcctacttca aagacgagtc cgccaattcc tactactaag ataactgaag caatcaacag
     9961 atttttcttt ttatcaaaat cgattttgtt ctcgattaaa atcttcattc cacttgcggc
    10021 aattactcca aataagataa aactgattcc tgagatgact ggtccaggga tacttaaaat
    10081 caaagcgctt aattttccta cgaatcctaa aatgactgca aatactgcag ctccaccgat
    10141 cacaaacacg ctatggacac gtgtgatagc cagaacacca atgttttcac cgtaacttgt
    10201 tacaggagga cctccgacaa aggctgccac gatttgtgct aatccatcac ctgtcaacgt
    10261 cttatgaaga cccggttctt caaagaaatt acgtttagtt agtttattca ataccataag
    10321 atggccgata tgctctgtca tcgtcacaaa agcgatgggt gccattgttg tgattgctcc
    10381 aagatatagc tttggttcat attgtacaaa gggaatctca aagttaggaa cagcaaacca
    10441 ggcagcgtct acgattggct tagtatccac gattccaaac agcaaggcta cgatgtatcc
    10501 gctaataata cctagtaaaa tcggaatcaa tccgagaaaa cctcttagcc acatgttgaa
    10561 gaaaattgtt aatcctaatg tgatcagcgc tacagcaata tacttgaaat catacgtgcc
    10621 ctgattatac atagcatttg ttgctgcatt tgccgcaagt cccaacccga taaccatgat
    10681 gactggaccg acgacgattg gaggcaagat tttatccagc catgctgaac cgatcttttt
    10741 gatgatcagc gcaacaatca aatagactaa tccagttgtg actgctcctt gtgcaatcgc
    10801 tggataacca tctgttttca tcaaaagctg catcgctgca atgaaagcga agctgcttcc
    10861 taagtacgca ggtatctttc ctttagtagt aaataagtac accaatgtac ctagtcctga
    10921 actgactagc gcaatacctg gatcgatacc tactaagatg gggactaaca cggttgctcc
    10981 gaacatcgtg aacaagtgtt gtaaacttaa tcctacccaa tgtcctgggg ttgggcgatc
    11041 gtggatatct aacaccacgt catcattatg aaatcctttt ttctccgaca tgtttctctc
    11101 tcctctaatc ttcctctttc aaaatcatga tgcgatcttg tccatcaagc tcttgcatct
    11161 ctactaaaat ctcttccgcc cgagatgttg gaatgttctt cccaacataa tctgcacgga
    11221 ttggcaattc tcgatgccca cgatcgacca ggactgctaa tgaaattttt cttggtctgc
    11281 caaaatccat tactgcgtcc attgctgctc gaatcgttcg tcctgtatac aaaacatcat
    11341 caatcaaaat aacttctttt ccttctaagg aaactgggat gtcagaagaa tgaagttccg
    11401 gttcttctgg attttctttt ttgtcatctc ggtataaagt gatatccagt tctcctacag
    11461 gaatatcgat atcttctagc tgcttcaggc gttcggcaat tcttgatgca atgtagattc
    11521 ctcgtgtttt gatacctacc aatacgatat cttggatact atggtttctc tcgataatct
    11581 cataagtaat tcgagtcaat gctcgcttca tggttacctg atctactact tcttttgctt
    11641 gcattttcat tccccctact tttttgcata aaaaaactcc tgccctaaga ggacaggagg
    11701 aatgtttaga ctgagcgtat actgatacac tgatagctat actatgtcaa tgagcaatct
    11761 acttatggcg caccatacgt atccttgctc ccacccttct tagcctcacg ggactaaact
    11821 taaaggaatt ctattaagtt ttatgctttc gttgttaatt actataccgt tatctaaatt
    11881 aaaatgcaat gttttttctt aatttttcta aagttttttc aaaaacttct ggaagaggtg
    11941 cttcaaagat catcttttcg tttgtagtcg ggtgcgtaaa acctaacatt tgcgcatgga
    12001 gaaactgacc attgcctttc aaagttttct tcggtccgta tactggatct ccagctaacg
    12061 gataaccgat atacttcata tgcacacgga tctggtgtgt tcttccggtt tctagtttta
    12121 attcaaccag agtaaatcct tcgaaacgtt ccaatacagt aaaatgtgtg acagccgact
    12181 tcccatcttc tcttatcgcc tgcattttcc ggttttcctt cgaacggccg attggcgcat
    12241 tgatggttcc tttttcgtga ggaatctctc catggactaa agcaacatat ttgcgcaaag
    12301 aggtcttatc ttttaattgc tgagctaatg attcgtgtgc ctggtcattt ttggcaacca
    12361 ttagcagtcc ggaagtatct ttgtcgattc ggtgaacaat acctggacga ataacgccgt
    12421 tgatcgtaga aagatccttg atatggtaca tcagcgcatt gaccattgtg ccatgcgtat
    12481 gacctgctga aggatgaacg accattcctt gaggtttgtt tatcaccgct acatcttcat
    12541 cttcgtaaac gatcgtcaac gggatatttt ctgggaccac atctaatgtt tctggttcgg
    12601 ggatcaagac agtaatctcg tctccttgtt ttactttgta atttgctttg actggctgtc
    12661 cattgacaga aaccgctcca tttttcagcc acagctgaat ttgcgaacga ctgtaatcgg
    12721 tgaataaatt agtcaatact ttatcgatcc taccagattc atggtgaatc gtcgttttaa
    12781 gttctgtcat ttttttcttt gatccttttc atctaaaata atataaatta acatacaaat
    12841 cactccacat acaagtgtca catctgctac gttaaaaatc ggaaagctca taaaatctgt
    12901 ctggaacata tctactacat agccaagacg aatacggtcg ataaaattgc caatcgcacc
    12961 agctagaatc aaactcaatc caatcatgaa ccagcgatta cctttttcac gatttttaat
    13021 catcaatgtg agaatcacaa cgactgcaat gagtgtcact atagtaaaga accacatctt
    13081 cccttctagc atactccatg cggctccagt gtttcgaata tgattcagtg acaaaatacc
    13141 tggtataaga tttttcgtct ctccaagcgc tagattatca gtgatccacc atttgaccaa
    13201 ctgatccact cctactagca aagcgctgaa tatccaataa ataattaaca aaaaatcaat
    13261 ccttttctat ttgtttataa atatcacgag gtcgtacgat cccgagcaga ttaccatcgg
    13321 gattcccatc tttagtgata agtacggctt ccaaagtccc ttgctcacga aacatcgctt
    13381 caacctcgta aatatgtgtt ttagctggaa caaaatgata atttgtgtac tttccgtttt
    13441 ggatgatcaa atccaacgct cgtttatttg ttaaatcaac cggacctttt tggctttctt
    13501 ttgctagcca aaacccgata ttacgtaaag taatcaatgc tacaaatttt cctttattgt
    13561 ataacggaaa ttgtgaatac cttttttctg ctactatctt taacagttca agtaacggaa
    13621 gatcttgttc aaaacctgtt acacgttttg caaatcgagg cagcacggtt tcaggctgaa
    13681 tcagttcttg ttcgatcagc tggattcgct tgactgtcca atcatttggc tctgcaatga
    13741 caaaatcgac tgcgatccga tcatggacga tcgcattcct taactgagca agctgtaaaa
    13801 gatcatcttc gtattttctg atcattaatt gttttctttg agctaggcgt ctgacaagtt
    13861 cggtaaatcc catatttcgt gcatttccca tctcgtctct catccatttt tcgattcgat
    13921 tgaaactgat taaaaaaaga tcagagtttg ccataatttt cctccttgat caaatatgcc
    13981 cggtttttta tatagaaaaa gaacgcaatc aacacaataa ctgcaaaaaa actgaaaaat
    14041 aaattttgta ggaaaacggc ccccaaccca gttgctagaa ataaaaacgg atagatttga
    14101 agaatcaagt aattcattgt gatcacataa gcaggctttg cttctttcac tgtataaacg
    14161 gggcttttta gccaataaaa ggtgacctgc atcgtaaagg gcacttccag atcaagcgtt
    14221 atcttttgtt catgactgag aacactgcgt ttttcattgt tgattcttag ctgaatcgag
    14281 ctacccatac cgtaaaaacc ggtttttctt ataaatgtcg ttttcatcat accctcctat
    14341 tatgctttag tatagctcaa aatacaataa agaaaaagaa tgatcctagt gtaaacaaaa
    14401 ttaattccct atcgtctaaa aaaagaagct gtagtcaaaa tacagctcct tcgatggtag
    14461 attataatca agcttaaaac aatcccaaca cttctccgtc atttgtcacg tccatattca
    14521 atgcagcagg ttttttaggc aaaccaggca tcgtcataac atctccagtc aaggctacta
    14581 aaaatcctgc ccctaatttt gggatgattt cgcggatcgt gatggtgaaa tcttccggtg
    14641 cacctagcag tgaaggatca tcagaaaatg aatactgtgt ttttgccata caaatcggca
    14701 gtttgtccca tccattcttc gtaaactctt ctatttgttt ttgcgctttt ttgctgaaaa
    14761 caacaccatt tcctccgtaa attttcttga cgattgtttc tgtcttttct tgtatcgttt
    14821 cttctaggcg gtataatggc ttgtaattag cttcttttct atcaatcaat cgtactacag
    14881 cttttgctaa atcaacgccg ccttctgctc catctgccca tacactcgct ctcttggcaa
    14941 gaatcccctg attttcacac aagtgctcca acagtgtgag ttcattgtct gtatctgtta
    15001 cgaattcatt gattgcgacg atcacaggta gctgatattg ttccatatta cgtatatggc
    15061 gctttaaatt tgcaaaacct actttcaaag catcaagatt ctcattttta agttcatctt
    15121 ttttcattcc gccatgcatt ttcaatgcac ggacagtggc gacaatcacc acagcatccg
    15181 gcgctttttt caagttaggt actttgatat ctaaaaattt ctctccgcca agatctgcac
    15241 caaatcctgc ctctgtgaca acgtaatctg ctaatctcaa tgctgtcttg gtagctaaaa
    15301 tactattgca gccatgagcg atattggcaa acggtccccc gtggacaaaa gctggtgttc
    15361 cgtaaattgt ttgtacaaga ttcggcttga ttgcatcttt cagcagtaat gccagagcgc
    15421 cttctgcttt caagtctcca gcagtcacag gctgacggtc aaatgtatag ccgacaacga
    15481 tccttgcaag acgagccttt aaatcttcaa ggtctgccgc aagacacaag atagccatga
    15541 tttcgctggc aactgtgata tcaaacccat cttctcttgg aacaccttgg attggcccac
    15601 ccaagccaac aattacttta cgcagttcac gatcattgag atcgactacc cgtttccaaa
    15661 tcactcggcg ggcatcgatg ttcaattcat ttccttgatg aatatgatta tctaaaagag
    15721 cagataatgc attattagct gttgtgatag catgcatgtc accagtaaaa tgcaagttga
    15781 tatcttccat aggcaatacc tgtgcatatc ctccgcctgc agcaccacct ttgattccca
    15841 tcacaggacc aagtgagggt tctcgcaatg caataacaga ttttttattg attttattga
    15901 gcgcgtctcc taaccctaca gtgatcgttg actttccttc gccagctggt gttggattga
    15961 tggaagtaac taagatcaaa tgcccctcag cgttatcctt tgatcgattg attccagaaa
    16021 aattgatttt cgccttatat ttcccataca gttccaaatc gtcttcctta agatctaatt
    16081 tactagccac ttcaacaatc ggttctagct ctgtttcttg tgaaatttgc agatcattct
    16141 tcatttgatt accccttttc acgaacttta ttcaacaaaa tatcttcttt gttaagatat
    16201 gccaattata ccttttttta ttcagaaaaa aaacataaaa ttacaggttt ttaatttatt
    16261 tgacgtactt ataaatactc tttataatga aggctatgat cagactttag aaaggtcgtg
    16321 agtgctttga ccacaggaat tgtaaaatgg tttgacaaca aaaaaggata cggctttatc
    16381 agttatgatg acacagaaga aatttttgta cattttactg cgattgaaga agagggattc
    16441 aaaactttgg aagaaaatca agtggtcgaa tttgagatca tcgaagggaa tcgaggaaca
    16501 caagctgccc atgtcaagaa aggcaacgtt cagactaccg atactagtat gcatgaatag
    16561 ataaacaaat agagtgagac gctaaacggg atgaaccgta cgtctcactc ttttcttttc
    16621 ctgtaaaaat tcgttttttt aggttctgat ttttaaaatt agctttttct ttttttcgta
    16681 taaggataag gaattgattc tgatttgggt ccgttttttt caaaatgatc aaaaaggact
    16741 acttgatctt tttttcgcat catgaaaaca gatggggctt tctcatcttg caacatcatt
    16801 tgcaccccag tattagttgg cgtcaaagat cgaatctgtc cgacagatat ttctcggtct
    16861 ttccaaaaag cagcataacg tataatcaca gagtttgatt ttaaaataaa agaacgccgc
    16921 aaacctagaa atagaaaaac aagaaaaata cctaaaacaa aattgctttt ccaataagga
    16981 cctgtgtttt ctaaagaaag aatcagacta aaaaacagaa tgatcaaggt caatgaccaa
    17041 tagatgatcg tatgtgccag ttcaggctgc catcgaatat tttttttcat tctctaacct
    17101 ctcaccgatt cagtaatgat ataatcttac cataacatta gtttgaacaa aaaggagaat
    17161 ctctttttat gttaaaagta tacattgatg cctctacaaa aggcaaccct ggtcctagtg
    17221 gaggtggaat cttgattgtc tatcaaggta aacaagaaca gctatctatc ccgctttcta
    17281 tagggacaaa ccatcaagca gagttcgaag tctttttaaa aactttagaa tttctaaaga
    17341 aaaacgactt acagaatgaa acgattttct gtttttcaga cagcaaaaca ctagtttcta
    17401 cggtcgacaa aaacgcaacg aagaacgagc tcttccttcc ctaccttcaa agaatccaag
    17461 aacttctatc cgaattttct ttattgatcc tgcaatggtt gcccgaaaac aaaaataaag
    17521 gagcagataa tcttgcacgt caggctctcc ataaacaatt aaaagaaaaa aagactcttt
    17581 aaattaaaaa ctttaaggag tctttttata gtttctcatt taatataaaa gataattgtg
    17641 atcaaacgta atttccaatt ggtaatctcc gattttttct tctatttgac tacgtaagat
    17701 tctttctagt tctgtatcaa caaactggaa tttctcaggg accactacat caaattgcag
    17761 cgtttttcct tgatgaatta ctctaaaatc atgaatatta agccctgtac ctgtttcaag
    17821 aagaatttgc tttaactgcc gcaagatatt atagtatttt tcgttatcaa caggcattgg
    17881 atccaagtga cagacaagtt ccacatccaa agacttttta aagtcttttt ctatcagatc
    17941 aataatctta tgagccacat tcaaattcat tgattcatct acttcgatgt ggactgatgc
    18001 aaagcgttta ttgggtccat agttatgcac caataaatca tgatagccaa gtatatcatc
    18061 gcaagcattt aaatgctctt ccatttcttc aatctctgct ggcgttgggc gacttcctaa
    18121 tagttcgtat acaaaatttc gaagcatcgt caatccacta taaaggatat ataatgctaa
    18181 agcaaaacct atatatccat caatacgcca tccagttacc cattcgattg ctgctgaaag
    18241 caatacagcc agtgtagtaa agacatcatt ataactatct gttgctgtag ctctcaatgt
    18301 ttcagaatca atggattgcg caataacctg atacattttg ccttgtgcaa atttcaaaaa
    18361 gatggaaatg attaggacaa aaaagacgat gggtgttaaa gagacatttt ctggatggat
    18421 gattttttca gcagaagact ctaaaaattg aaatcctaca taagtaatga tgatcgaaac
    18481 aaataaccca ctgatatatt cgaagcgttc gtgtccataa ggatgttcct gatctgctgg
    18541 ctttgcagca attttaaatc cgatcaaagt caatatggat gaagcagtat ctgatagact
    18601 gttcactgca tcagccataa tggagacact tcctgaaaaa agcccgatca ttagcttaga
    18661 gacaaacaag atcagattcg taaccaatcc taagataccc gctagtactc ctttttttgt
    18721 ccgctgttgc ggtatttgtt tctttttttc ttcttgtttc ttagccaagt tttttacccc
    18781 ttctagtttg cttgttgctc aggtaaaaag atggagagga cgattgtttt cctctcctct
    18841 cttccttttt ctatctatcg tgcgtcctat tactattaaa agatggtcat tattctggtt
    18901 tcaacggtgt tgctgcaaat aacgcttttg ctagtaatgg tgttatgatt cccgcaccta
    18961 ttgcttctgg tatcccattg attgctgcaa cccctgctaa ggttttaaat aaaagagcag
    19021 agtctacacc ataggcatct gccgttgcac ctgtataaaa taatcccatg aatcctaaca
    19081 ctaagactgt attgaccaat gaacccagta cccctgcaaa ggctgagctg atcgttattg
    19141 atttattttt ttgataaatc cagtggaaaa ccaagcctgc tactaagcca acaagtactc
    19201 tgggaactac cgagataatc ggattcgtga acactaatgt atcaaatggc gttgttggca
    19261 tggcataggc acgaacaatt gtagcgattc cccaaaacaa gccaatcacc atgccatctt
    19321 ttgggcctaa tactactgca gcaataatta ctgtgatatg gataattgtt aaactaatga
    19381 atcctaatgg aataaacccc agccaaggaa ccatcgcttg tacgatgatg attgctaaca
    19441 agatcgcacg taagactagt cgaaaggtct tatttctact attcattttt tcaccccttt
    19501 ttatttatac taatcgtaaa aatttctgtc actttcgtca actaaagacc atccgaatcc
    19561 gaccatttga ttagatgcgc catccaattg aaaaaagtat gagggcaaga cttgtttgtc
    19621 ttcgccccca taatcatgtt tcgttcaatg tttgaacttt tgtttttctg tcaaacgtaa
    19681 acgccctatt gttgcgcaac ccattctttc gcagctttaa gtgcatctgg aataccagca
    19741 ggatttttcc cgccagcttg tgccatgtct ggacgtccac caccgccacc gcctactttc
    19801 ggtgcgattg ctttaatcaa gtcgccagct tttagtccag cttcatttga ttctttcgtc
    19861 attgccgcta gcagactgac ttttccttct tgttcgtttg ctaaaacaag aatatctgaa
    19921 atattttttt gtttccattg gtcagctaat tgacgcagct gattcatatc tttcactttt
    19981 acttgtgctg caatgattgt ttttccattg atcgtttctg gatctttgaa tacatcacca
    20041 gcttgttgat tcgctaactt gctagctaat tgttcatttt ccttttgtag ctctcttact
    20101 tgctgttgca attgttccgc ttttgaaaca acttctttca attgaggtga tttaacaatt
    20161 gttgctactt gttttagttg tttttcttct tcttcaagta gttcgtacgc ttctttactt
    20221 gttacagcct cgattcgtcg cacgcctgca ccaattcctg attcagaaac gattttgaag
    20281 attccgatgt tttctgtatt tgctacatgt gtgcctccgc acagttcgat tgaccagccg
    20341 ccaatattta ctactcgaac gtcatgtccg tatttttcac caaacaaagc cattgcaccc
    20401 atgtttttgg ctgtatcgat atctgtttca attgtttcaa ctgggatagc ttcccagatt
    20461 ttttcgttga cgattctttc catttcagct aattcatctg aagtgatttg accaaagtgg
    20521 gtaaagtcga atcgtaaatg tccaggagcg actaaagaac cagcttgatt tgcatgctcg
    20581 ccaagtactt ctttcaatgc cttgtgcagt agatgggttg ctgtatggtt tttgatgatc
    20641 cgattgcgca tcgattgatc gatcaataga tgataggttt gtccttcact cattggtgct
    20701 tttacttcta cggtgtgcat aaattgacca ttaggcgctt tttgaacatc tgtaacaact
    20761 gctacgattt cgcctacttc attcatgatc catccttgat ccgctacttg cccacccatt
    20821 tctgcataga aaggtgtttg atcaaagatc atttgagctt caccttctgc gacttcattc
    20881 aaaagagact cacctttaac gatgaccaat aattgactat ctgattcttg ttctgtgtat
    20941 ccaatgaatt tactttctac tttgatatct gtcaataaag cagattgtac ccccattgat
    21001 ttttcagtac tgcgggcaga gcgagcacgt tcgcgttgtg catccatttc tttttcaaaa
    21061 ccagcatgat caactttcag tccttcatct tctgccacct cttccgttaa ttctaccggg
    21121 aagccatatg tgtcgtatag tttgaagatg tcttttcctg ctagagtcgt cccgttttcg
    21181 ttttttactt cttggatcac ttgatttaag atttccaaac cttcattgat tgtttcattg
    21241 aaacgttctt cttctgtacg gataactttt tcaataaatt ctttttgttg aagtacttca
    21301 ggataataac ttaccatgat ttccccaaca acagggacta aacgatataa gaatgcttgt
    21361 tcaataccta attttttccc atgcatgaca gcacgacgaa gcaaacggcg taagacatac
    21421 cctctccctt cattcgatgg caatgcaccg tcgccaatag caaatgaaag ggcgcgaata
    21481 tgatctgcga ttactttgta agaaatatct gttactgggt tttcaccata ttttacttgt
    21541 ttgcttagct tttctacggc gtggataata ggcataaaca agtctgtctc aaagttcgtt
    21601 gacgcatctt gaacgattga gaccatgcgt tccaatccca tccccgtatc aatgttttta
    21661 tgaggaagtg gttcaaatgt gttatcaggc ttatggttga attctgaaaa tactaagttc
    21721 cagatttcta aataacgttc attttctcct cctggatagt tttcaggatc gtcttctgct
    21781 aaatcgttat aagcttctcc acggtcatag aaaatttccg tatccggacc acatgggcct
    21841 gcaccgatat cccagaagtt gtcttctaca tcaataatgt gatctagtgg caggccaact
    21901 tcttcatgcc agatgcgttt tgcttctgta tcttttggat aaactgttac ataaagtttt
    21961 tctggatcga atgccatcca ttttggatca gtcaaaaatt cccatgccca gtggatggct
    22021 tcatttttga aatagtcacc aatcgaaaaa ttacccagca tctcaaacat cgtatggtgc
    22081 cgtgctgttt taccgacatt ttcgatatca tttgttcgaa tagatttttg ggcatttgtg
    22141 attcttggat tttctggcac aactgaacca tcaaaatatt ttttcaatgt tgcaacacca
    22201 gagttgatcc acaataaggt tggatcgttc acggggatca aagacgcact aggttcaatc
    22261 gtatgtcctt tagattggaa gaaatcaaga aacatttgtc gaacttgtgc actacttaat
    22321 tgtttcatgt cattacctgc tttctataaa ttaaatacaa aaaaacacca tcacagccaa
    22381 ggacgaaaac tcgcggtacc accttgattg caatgatgtg taatcattac ctcttaaact
    22441 cgataacgct gagctgcgtc ggtatttcta ccaaaataga aaaagggagc aatttgatta
    22501 gtgactattc ttttttcagc caagaaagaa ttttctgtga gtcttaaaaa acaaatcata
    22561 tccttttcgt acttaattag tatacgcatt ctattgatca ctgtcaatat ctcaattttt
    22621 attttcattt ccttttggtc atactccacg atttttggta cgataagaat aaaggaatca
    22681 atacatctct gctattcatg acaaggagga atatgatgaa aaaaatagtt ggagcggatg
    22741 aatggatacg cgcagctagc tgctttttga gatatcaagt ttttgtatta gaacaaggga
    22801 ttcctgcaga actagaattc gacggaaaag atatcccgca acaaacctat tatctatttc
    22861 ttgatcaaaa aagaccagta gctactttgc gttatgaaaa gaaaacacct acttgcttca
    22921 agccggaacg attttgcgta gcaaaagaat accgaggaaa agggttaggc ggtcagttgc
    22981 ttgaagctgc tgaaaaacgg gcggtttcag aaggttgtca gactagttat gtagtagctg
    23041 aaatgacggc tgtagatttt tatgaaaaac aagggtatca tacttgcaca gaacctttta
    23101 ttgaagatga agtactttgt atcggaatga aaaaaagtct agcttaaaaa caagagattg
    23161 tgacacgcct tttgtcacaa cctcttgttt tttatttatg tgaatttttc ttatttttac
    23221 gagcagtacg gctttgctgt ctgcgttcga ttttgcgttc ttttttattt ttttctgcaa
    23281 ttgcccaatc gatttttttc ttataaccag gcttgatctt tttctttttc ttcttcacaa
    23341 gaccaatcag cgtgggatct aatacatctt ttgatttttc tcgtttggca cgtctattac
    23401 gatcgtatgt tttgacgatt tccccgtttt tgatttcttt tggttcaaat gagacgccca
    23461 atttttctat cgcatcaatg gcttgctcat catttggcga atataacgta atggccgttc
    23521 cttttaagcc attacgtcca gtacgtccga cacgatggat aaagaagtct agatcttctg
    23581 gaatttctgc attaataaca tgagagacac cttcgatatc gatccctcta gcagctaaat
    23641 cagtcgctac aacatattga tactccagat tctgtacttg tcgcatcact cgttttctct
    23701 ctcttggggt gatgtctcca tgaatctttg ctactttcaa gccttggttt ttcaagtaat
    23761 cggcaatttc atccacatgc tgttttgtat tggcaaatac gattgctaaa taaggatgac
    23821 caattgtcag cagttgataa atcatttgat tctcatcttt accttttgtt gaaatcagcc
    23881 aattttcaat gtcttcagaa ataactgctt ttggtttgat ttcttcaatg attggatttt
    23941 ccatgtattt tcgtaagaat ggacgaagtt tttctggaat agtggcagaa aaaactaaaa
    24001 actgcagctt ttctggtaat cgacttgcaa tttggtctac ttcatttaaa aaacccatat
    24061 ccaatgtcat atccgcttca tcgacaacaa atgcaaatgc ggtgtgaatc ttcagcgctt
    24121 gttcattcat catgtctaat attctaccag gcgtgcctac tacaacctgt ggctgttggt
    24181 gatgcagacg atttaattga cgctgtttgt ctgtaccgcc gacaaaattc gagacacgga
    24241 tctccggtgt agaaaattta gcgatttgtg tagctgcttc atagatctgt gcagctagtt
    24301 ccctacttgg agctgtaata acaacttgta cttcctcttt ttctgcattg attttttcaa
    24361 acaaaggcaa caaaaacgta tgtgtttttc ctgtaccagt ttgagattgc ccaatgatat
    24421 tttttccttt tgcaattaaa ggaatcaaac gttcctggac ctctgtgggc tgtttgaagc
    24481 cctttgcggt taacgcttca ttgataaacg gtttgaagcg gaatatatca aaattattca
    24541 ttcgtgcacc tcttctcttt aaattatcac gactttggca ttataacaca gacaaagcgt
    24601 ttttgctcag ctaatttcga gaatcatcat cctttgtttg gccaatcgtg ctaacagtat
    24661 atgtgtgact agctttttca taaaacagaa tccgtttttt tctcaaaaat gatagcaaga
    24721 tatttaaaat aaaaatacct tgtagtaaca agacaccgag agaaatgaat actgcactca
    24781 tctgcaacac cgtattcgtt gtcgaattca tgatttgctg aagcaactgc cccaaccagc
    24841 ttaaaccata aaaaagaaag tacagatacc cgtagcggat catcaatgct tttaaagaaa
    24901 ttcgttcgcg gtcttcttcc accaaacgga tacgcacaat ttttttgcca agtgtccatc
    24961 cattggttat aaacggtaca atcataaagt atacccctac ttctaataaa agccaccaga
    25021 tatgagaagt aactgcccct gaatcgaacg caattttaaa gaaaatcgcc gctaccaaat
    25081 taataagaga taaaaatgtc cagtcgatca aataagcgac caagcgtcga gtcaatgaga
    25141 cagtctttcc tttttcataa gaaagttcat ccattcgtct tcttgtagga agcataaatg
    25201 taaataacgg cgtaacgaca aaaccaatga ttccacccaa tgtatttgtc actaaatcat
    25261 tcacatcaaa taagcggtat ggtcttgggt agataaaata aagtccactt agctgcgtca
    25321 attcaaaaaa tagagaaagg cagaaactag caatcatcgt tttcataaaa gaaagccgga
    25381 aataatatct taaataaatg ccaaacggta tgagtaataa aacattaaaa gctggttcca
    25441 aaaatacact ctgcttcatt gccggcaggt aggtactagg ttcagacaag accaataccg
    25501 tgttgtctct gaaatgttgc catgaagcaa ataaatgcag ctccatcttt ggccctgtca
    25561 tttgggcaac cgtctccttt gccggaagtg gtaagatcac caaaaaatac gcacacaata
    25621 aataaaaaac gaaagaatac aaaataactg cccttgttac tagaaatgtt ccatactttc
    25681 gatattcata aataaaaaaa acagatgaga tcgctaaggc taaaaatgga aaaacaatca
    25741 gcgcaaaacg aatcggttcg atataacttg ccatatgact cctccttgat tctttgtatc
    25801 tctcttatct tagcagtcct ttgctgtttc aagcaagtaa cgaggctatt atttctcatt
    25861 tctttcaaag taaagtaaga taaacacaaa gggggtgtcg agatgctgaa tatgtacttt
    25921 gtttttggcg tacctatttt tttactgttc ctttatgcta cgatcgctta tgtaagaaaa
    25981 agaactacaa ttcattattt aggatttatt ctcctgatta tttcgggttt catgctagtc
    26041 ttcaatcttc agacttggca gcaagcactt cttgaaatgg ataaaatgac tccacatgct
    26101 ttgtctaaag tgcttggcta ccctgtctat ctgatttggc ttcccatttt catttctggt
    26161 tgcttggttt tattaaatat ttatcgcggc gtacgccgaa tagttcagtt acgcaaatca
    26221 aagtaaaaga cagaaatcgg ttgagcccga tttctgtctt ttactttgct atttcatcgt
    26281 agacaaaacg acatttacgt gatcaaaatt aacgagtact tttgacattt cttccacgta
    26341 ctcctcattt aaataaaata ttttgtttgg cagagaagtg cagttctaat aaaattcgaa
    26401 tgttttagct atctacgttt taaatagaga taaatcggga taccagcaag tgtcaatact
    26461 atccccatcg aagctaaaaa ggtctgagtg ataagcgtgc ttgatacgat aaaagtccct
    26521 ccaatgattg ctactagtgg aataaacgga tacattggaa ctttgtatgg tcgaaacaaa
    26581 tcaggttctt ttttacgaag aagaatgaca ccaacaaaga ccatcgtata aaaaatccaa
    26641 atgacaaaga tcagcatatc cgttagtgta tcaaaaccac cgatcatcat catgccaata
    26701 gcgatgacta gctctaagat acctgcggct acaggaactt tagttttatc atgaagttta
    26761 gcaaagagct tgctaaaagg caggttgttt tcttttgcca taacataagg gagacgcatt
    26821 ccagtaaggg tatagccatt gatggttcca taaacagaaa tcaagatacc aactgtgacc
    26881 agtcttccac cgaagccgcc aaaaatcatt tttgcaacgt cacttgccgc attgctgttt
    26941 cctgcgactc cgtcgataga tgcggttctt aagaacaccg catttactaa taaataaacg
    27001 atcatgattc cgatgattcc taaagaaatt gcttttggca aatctttcgc cggctttttc
    27061 aattctcccg aaatatttcc tacgtgtatc caaccgtcgt atgcgaacat cgttgctagc
    27121 aagcccgctc ccaaggctga gaaaaatgaa agattttctc ctgcttggat cggaaacagt
    27181 tggaagtcta ctccttcttg tcggaacaaa ccgaaaatga cgatcacaaa caaaggaata
    27241 agcttgcaga caagcgtaat cgactgaaaa gcccctccag ctttagaacc taaaaaattg
    27301 atcaacagga tcgatacagc agctgtaaca gctacgggaa caatcattga ctgggaaaga
    27361 ccaaatagat tgacgaactg tgtaccgaaa atgatcgaca aggctgctac atttgctgga
    27421 aaataaatca cgacttgcgc ccagcccaat aaaaaagctg ctgtatttcc gtaaatccgt
    27481 tcgatatatt taatcatccc tcctgtttct ggaatagcag ctgccagttc agctcctgtt
    27541 agtcctgcac agacagaaat cataccaccg agaaaccaag aaaacatatg caaactggcg
    27601 gatcctgtaa cttctgctac gcttgcggct ttaaaaaata cccctgcacc gatcactgtg
    27661 cccataacag tcgataaagc agtaaaaaat cccatcgtac gttttaattc attttctatt
    27721 tttacttctt ttactggcgt ggcttgatat tcttccatcc cttgtcccct ttcataccat
    27781 agccaatccc aaaagtataa aaaacttttt ggatgtggtt cttgttttat accgtctcta
    27841 ccaatttttc tttcagtttt tccaacatat cctttgtcat cacatctaag tcgtatttcg
    27901 gcgaaaagcc ccattcctct tgcgcacaag aaacatctag tgaatttggc caagattctg
    27961 ctattgactg acgtacagga tccacatcat aatccatttc aaaatctggt atgatttttt
    28021 gaatagatgc tttgatctct tccgggtcaa aagacatcgc cgatatattg aaggcatttc
    28081 gatgaaccaa tcgatcagga tctgcattca tgagttttat aatggcatcg atcgcatctg
    28141 gcatatacat catatccatt gatgtacctt gatcaatgaa actagtgtac tttccttctt
    28201 tgattgctga ataataaata tcgactgcgt agtctgtcgt accgcctcct ggcaaggttg
    28261 cataagaaat caatccaggg aaacggacac cacgagtatc tacgccatat ttcacataat
    28321 aataatcaca tagtagttct ccagctacct tcgttacccc atacatggtt gtaggacgct
    28381 gaagagtatc ttgtggggta tgatcttttg gagtatttgg accaaacgcg ccgattgaac
    28441 ttggcgtaaa aaatttcagc tggaattctc tagcgacttc caatgcattg accagcccag
    28501 tcacgttcaa attccaggct aattgtggtt ttgcttctgc aacagctgac aaaagagcag
    28561 ccaaatgaat caatgtgtcc acttgatatt gttctgctat gctgcgcatt ttttcgtaat
    28621 ccattacatc taggatctca aagatatcgt ttttctcaat gacttcaggc atgcgtatat
    28681 ctgtcgcaat gacattttca ctgcctagtt cttgacgtag ccgtgtgatc aactctgaac
    28741 caatttgtcc taaacatcct gtcaccaata cttttttcat gatgatcctc cctgatcttt
    28801 tttactacta tcttcagatg attaacaata tatgcgtgaa catgttctct aattttaaat
    28861 agacgaaaaa actaaattta gttaaataat ttccatttct tttcctattt tttgataaac
    28921 tgctactgct tcatcaagca tttccctcgt atgttccgca gtcggcatat ttctgatcct
    28981 gcctgtccca agaggcacag ttggataaac gattggttta gcataaatcc catgttcgat
    29041 cagcttctta gagaaagtct gagtcacttt ttcgtctccc aaaatgactg gagtaatagg
    29101 tgtttcagac ataccgatgt tatagcctac ttttttcaat tcttctttaa aataattagc
    29161 attttcccaa actttttcga caagttccgg atgttcttgc atcaaagtaa tcgaagccaa
    29221 tgcagctgca gctgcacctg gagttagcga agtagaaaat aggaatggcc gacttctagc
    29281 tttcaaccag tcgattagtg ttttcgatcc agctacataa cccccaacaa cacctatagc
    29341 tttcgataac gtgcccattt gcatatcgat tttgtcgctt aatccaaaat gcttgacggt
    29401 tcctgcacct tttccagtta cgcctgagcc gtgcgcatca tcgacgtagg tgatcagtcc
    29461 atatttttca acaatcggaa taatttccgg caatcgtgca atgtctccat ccatggagaa
    29521 aacaccatct gtgatataca tgattttttt atatttctta ctttctatcg cttcctttgc
    29581 ttttttctca aggtctttca tatcttgatg tttaattcgg atgatttttg ctccggataa
    29641 gcggcaaccg tcaataatag atgcatggtt taattcatcc gataagatag catcttcttt
    29701 tgtcattact gcagagatag cgcccatatt gcagttgaat ccagattgaa aggcgattgc
    29761 tgcttctgtc cctttgaatt ctgcgatttt ttcttctaat tgctgatgga ttttcagcga
    29821 accattgatc gttcggactg ctcctgctcc tacaccataa gtttcggttg cttcaataca
    29881 tgcttttttc aattcttcgc gattggcaaa tccaagataa ttgttggatg ctagattgat
    29941 catctttttt ccgtctacga tgatacaagc accattttca ctttctagta tgtcgatcgt
    30001 attgtataac cccttttctt tcagttgatc caattctttc tctaaataca ttgttagttc
    30061 tttggaagac attgtctcca cctccatatt catctgtctt gcagaacata ccaaacatat
    30121 atcgtgtcat tcatctacaa agcaagtatg ctatattttt gataacgctt acactgatta
    30181 tgagaattat ttaaccacat tttttattta cagtatcttt ttactttcaa atgacttata
    30241 tagtatgaac aattaccttt tagataatga tctattttac tctacctggg cagataaata
    30301 taacacaacc attgatagaa gccctttcgt taaaatcaat tgtattctga atatttaatt
    30361 tatttattgc atagaccaat aaaaatacat aaaatttcca tcttcgttta tcagcaattt
    30421 ttaattttac tctctgaata tttcttaaaa gttttacttg ttgaattcga aaaaaggact
    30481 gtgacaaaag tcttgtcaca gtcctattct tccgaata
//
LOCUS       DAZUKA010000016        30213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000016 DAZUKA010000000
VERSION     DAZUKA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.016"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1118
                     /locus_tag="ACG8PN_000581"
     CDS             48..1118
                     /locus_tag="ACG8PN_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860761.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1133..1801
                     /locus_tag="ACG8PN_000582"
     CDS             1133..1801
                     /locus_tag="ACG8PN_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860762.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1821..2399
                     /locus_tag="ACG8PN_000583"
     CDS             1821..2399
                     /locus_tag="ACG8PN_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4860763.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2800..3711
                     /locus_tag="ACG8PN_000584"
     CDS             2800..3711
                     /locus_tag="ACG8PN_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4860764.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4089..5006)
                     /gene="rnhC"
                     /locus_tag="ACG8PN_000585"
     CDS             complement(4089..5006)
                     /gene="rnhC"
                     /locus_tag="ACG8PN_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4860765.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5208..5645
                     /gene="zapA"
                     /locus_tag="ACG8PN_000586"
     CDS             5208..5645
                     /gene="zapA"
                     /locus_tag="ACG8PN_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4860766.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5703..6251
                     /locus_tag="ACG8PN_000587"
     CDS             5703..6251
                     /locus_tag="ACG8PN_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS4860767.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6366..8726
                     /locus_tag="ACG8PN_000588"
     CDS             6366..8726
                     /locus_tag="ACG8PN_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4860768.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8813..9127
                     /gene="trxA"
                     /locus_tag="ACG8PN_000589"
     CDS             8813..9127
                     /gene="trxA"
                     /locus_tag="ACG8PN_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4860769.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9309..11087
                     /gene="uvrC"
                     /locus_tag="ACG8PN_000590"
     CDS             9309..11087
                     /gene="uvrC"
                     /locus_tag="ACG8PN_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4860770.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11232..12131
                     /locus_tag="ACG8PN_000591"
     CDS             11232..12131
                     /locus_tag="ACG8PN_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860771.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12124..13386
                     /locus_tag="ACG8PN_000592"
     CDS             12124..13386
                     /locus_tag="ACG8PN_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860772.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13547..15445
                     /locus_tag="ACG8PN_000593"
     CDS             13547..15445
                     /locus_tag="ACG8PN_000593"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4860773.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15775..16494)
                     /locus_tag="ACG8PN_000594"
     CDS             complement(15775..16494)
                     /locus_tag="ACG8PN_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4860774.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16684..16731
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16831..17163
                     /locus_tag="ACG8PN_000595"
     CDS             16831..17163
                     /locus_tag="ACG8PN_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860775.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17150..19153
                     /locus_tag="ACG8PN_000596"
     CDS             17150..19153
                     /locus_tag="ACG8PN_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4860776.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19172..19642
                     /locus_tag="ACG8PN_000597"
     CDS             19172..19642
                     /locus_tag="ACG8PN_000597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4860777.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19663..20247
                     /locus_tag="ACG8PN_000598"
     CDS             19663..20247
                     /locus_tag="ACG8PN_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS4860778.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20262..21248
                     /locus_tag="ACG8PN_000599"
     CDS             20262..21248
                     /locus_tag="ACG8PN_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4860779.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21238..21549
                     /locus_tag="ACG8PN_000600"
     CDS             21238..21549
                     /locus_tag="ACG8PN_000600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4860780.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21571..23352
                     /locus_tag="ACG8PN_000601"
     CDS             21571..23352
                     /locus_tag="ACG8PN_000601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4860781.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23345..24721
                     /locus_tag="ACG8PN_000602"
     CDS             23345..24721
                     /locus_tag="ACG8PN_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4860782.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24728..25363
                     /locus_tag="ACG8PN_000603"
     CDS             24728..25363
                     /locus_tag="ACG8PN_000603"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4860783.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25393..26748
                     /locus_tag="ACG8PN_000604"
     CDS             25393..26748
                     /locus_tag="ACG8PN_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4860784.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26940..28334)
                     /locus_tag="ACG8PN_000605"
     CDS             complement(26940..28334)
                     /locus_tag="ACG8PN_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4860785.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28426..29397
                     /locus_tag="ACG8PN_000606"
     CDS             28426..29397
                     /locus_tag="ACG8PN_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4860786.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29394..30176
                     /locus_tag="ACG8PN_000607"
     CDS             29394..30176
                     /locus_tag="ACG8PN_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4860787.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
BASE COUNT         9973 a         5066 c         6537 g         8637 t
ORIGIN      
        1 gtgataaaat tgttgtataa aatgattatt tcaggaggag gagttaaatg tttttagcat
       61 taaatgaaat tatgcattca aagttacgtt atgcgttagt tgcaggggtg atgtttttga
      121 tcgcctacct ggtatttttt ttgactgggt tagcttatgg cttagctcaa gataatcgaa
      181 cagcagtcga caagtgggag gcagattcga ttgtgctttc gaaagatgca aattcaaatc
      241 tagggatgtc catgattact aagaagatag cagaagaagt cgaaggtggg aaagtggcgt
      301 atcttgccca aacacctgga gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca
      361 atgttagctt cttcggtatc gataaaaatc aattcatcat gcccaatctt gttgaaggaa
      421 aagcttttga taacgatgat gaggcagtag gcgatattag tttgaaagaa gaatatggtc
      481 tagccatagg agatacagtt aagttatctg gtagtgacaa gacgtttaaa ctaactggct
      541 ttacggatca tgccaaattc aatgtctcac cagttctcta tacaaccatc aatgcttacc
      601 aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac gcaatagttg
      661 tcagaggtaa aatcagtaat ttacctgaag atttagaaca gatcaaaatt tctaaattca
      721 tcaatgaatt acctggatat aatgcgcaag tattgacttt tggatttatg atcggatttt
      781 tgatcgttat tgcggctatt gtgatcggga ttttcattta tgtactgacg atgcaaaaga
      841 ttaatatctt tggtgtaatg aaagcacaag ggatcacagg aggatttatt gcgcgttctg
      901 tagtggcaca aacattcatt ttatcatttg ttggtattct tcttggtcta ttaggaacag
      961 tgggaacatc ccttgttcta cctgatgcgg ttccattcca aagcaattgg ctgttttttg
     1021 gcgtcattag cttgttgatg ttagtagtag ctgtgttagg cgcactattt tctgttcgga
     1081 caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga aggaggaaga acatgaaagc
     1141 aatagaattt aaagaagtag aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt
     1201 gaaagaaaca accatgagta tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg
     1261 ttcaggtaaa agtacttttt tgactttggc aggcggattg cagacgccta gcagtggctc
     1321 tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg
     1381 ttttaaagaa attggcttta ttttgcaagc ttccaatctg gttccttttc tgacagtaaa
     1441 aaagcagtta ttattagtag ataagattaa acatgaacat cgtcaaaaag aagcaaacga
     1501 attatttgag cagcttggcg tggataagtt gatcaacaag tatccagaag aactatctgg
     1561 tggagaacgg caaagggtcg caatcgctcg tgcattgtac aatgatccgt ccattattct
     1621 agcagatgaa ccaactgcta gtttggattc agaaaaggca cgagaagtag tcgatatctt
     1681 ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg gttactcacg atacacggct
     1741 gattgacaaa tgcgatagaa tatttatgat ggaagatggt gtattgaaag aaaaagaata
     1801 accaaaaagc aggtggaaaa atggtccatc gatacaacaa agatacacag caaaaaattt
     1861 tgaaagaagc gactgacttg tttatgtcca agggatatct cggcacttca acaagagaaa
     1921 tcgcacaaaa agttgggatc acccagccca atctctatca ttatttcggt gataaagaaa
     1981 aactatatac ggcagtttta gaaaaccact taaaagatgt tggaaaagct ttgagagaaa
     2041 tcgttcaaac aagcgaaacg ggatttcaag taaccttgac aaaaatggcg cagtttttga
     2101 ttgagacaca tctggtcgat ttgtttctga tgcttcacga tcttgaaagc aatctttcaa
     2161 aggaaacaag agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag
     2221 aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa
     2281 ggcatttttt cttagtgtta gcaccttaca tcactcggtc gaaaagacct gtggaagaac
     2341 cgctaaatgt ggcgcaattg atcgatttat atttgaatgg tgtattagga gaagcgtaaa
     2401 gctgttatat agggagaaaa tagttaggtg aagaagcaag atatgacgaa gatcgttata
     2461 tcttgcttct ttatataggt agtttttcat aaatacaaat ttgttattct ctcatgatta
     2521 tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat ttaataacga
     2581 agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa
     2641 tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt
     2701 tattttctac ggttatatct tttacagccc cttttttgct tacttaagct gattttaagt
     2761 tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct
     2821 tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa
     2881 aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca
     2941 tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat
     3001 acagatgact cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt
     3061 ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta
     3121 gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct
     3181 gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat
     3241 gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg
     3301 ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt
     3361 ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca
     3421 gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa
     3481 gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga
     3541 ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc
     3601 gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag
     3661 cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc
     3721 ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg
     3781 caagctttta aacaccgttt aaggaagagg tcgtgagcct gccgttcaac tctgagtcac
     3841 aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
     3901 gagttggcat gagaacaccg tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt
     3961 attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg
     4021 aggatcagat gattgaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca
     4081 cagttccatt atttttttag tagcttcaaa gctttttctg tattggcaaa atgaagtttg
     4141 gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca
     4201 gatttcgttc cagctcctga cggaagggtg attccagctt cagctgatgc tttgtctaac
     4261 tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt
     4321 tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat
     4381 tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc
     4441 tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg
     4501 gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag
     4561 gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt
     4621 gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca
     4681 catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat
     4741 aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc
     4801 tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt
     4861 ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga
     4921 atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt
     4981 gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac
     5041 cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt
     5101 atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc
     5161 atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa
     5221 aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca
     5281 agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat
     5341 tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg
     5401 atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca gaattaaaga
     5461 aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga
     5521 acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg
     5581 tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta
     5641 attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta
     5701 gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc
     5761 gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc
     5821 agcattttta caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca
     5881 ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt
     5941 tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg
     6001 tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg
     6061 caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac
     6121 ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt
     6181 ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata
     6241 ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt
     6301 taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg
     6361 ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca
     6421 gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg
     6481 agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc
     6541 tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg aaacggattg
     6601 agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga
     6661 caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt
     6721 tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag
     6781 caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac
     6841 agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga
     6901 aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc
     6961 cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag
     7021 gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat
     7081 accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg
     7141 ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg gatctgatga
     7201 atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca
     7261 atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta
     7321 atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg
     7381 ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac
     7441 cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag
     7501 gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg
     7561 tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag
     7621 gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa
     7681 agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc
     7741 gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc
     7801 gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc
     7861 ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa
     7921 acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa
     7981 tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct
     8041 atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg
     8101 tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc
     8161 agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa
     8221 atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa
     8281 aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt
     8341 tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg
     8401 aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg
     8461 tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg
     8521 aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc
     8581 aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc
     8641 ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg
     8701 gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc
     8761 aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca
     8821 agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt
     8881 ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca
     8941 agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac
     9001 tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt
     9061 agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata
     9121 tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg
     9181 gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg
     9241 tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg
     9301 agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct
     9361 gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa
     9421 aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta
     9481 gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag
     9541 agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa
     9601 cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg
     9661 tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga
     9721 caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg
     9781 agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc gacgtagatc
     9841 cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa
     9901 aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa
     9961 aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa
    10021 tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga
    10081 tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt
    10141 ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg
    10201 aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg
    10261 tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag
    10321 ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca
    10381 gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga
    10441 ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg
    10501 cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa
    10561 aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt
    10621 cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag
    10681 gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg
    10741 ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg
    10801 ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg
    10861 aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt
    10921 cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt
    10981 tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc
    11041 cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt
    11101 tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg
    11161 ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa
    11221 ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg
    11281 tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg
    11341 gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag
    11401 aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc
    11461 cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag
    11521 aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat
    11581 ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa
    11641 aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat
    11701 tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa
    11761 aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg
    11821 atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc
    11881 gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag
    11941 aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct
    12001 tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc
    12061 ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa
    12121 tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt
    12181 tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga
    12241 aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa
    12301 atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct
    12361 gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca
    12421 actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca
    12481 caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta
    12541 ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa
    12601 gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac
    12661 gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc
    12721 attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt
    12781 acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg
    12841 caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa
    12901 atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc attcttgtgg
    12961 tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt
    13021 ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta
    13081 tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt
    13141 attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat tcgcttagca
    13201 aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca
    13261 ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa
    13321 ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa
    13381 gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt
    13441 tgtatagacc tattttattt attttactat attggatata tattcattga aaatagtgta
    13501 taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt
    13561 agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct
    13621 cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga
    13681 atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa
    13741 agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc
    13801 ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat
    13861 ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg
    13921 aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg
    13981 tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga
    14041 agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt
    14101 gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat
    14161 catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc
    14221 gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta
    14281 tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca
    14341 tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag
    14401 agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga
    14461 acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa
    14521 aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac
    14581 ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt
    14641 gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt
    14701 tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg
    14761 tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg
    14821 aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc
    14881 aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt
    14941 attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca
    15001 cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat
    15061 tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt
    15121 aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg
    15181 aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa
    15241 ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc
    15301 aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact
    15361 caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct
    15421 gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga
    15481 gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa
    15541 ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc
    15601 cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag
    15661 ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat
    15721 ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa
    15781 ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc
    15841 cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaac
    15901 gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg
    15961 atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga
    16021 aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac
    16081 tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa
    16141 tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat
    16201 cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc
    16261 ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg
    16321 tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg
    16381 agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa
    16441 agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat
    16501 tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt
    16561 gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa
    16621 ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa
    16681 aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt
    16741 gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa
    16801 gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa
    16861 atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag
    16921 atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa
    16981 ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa
    17041 atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag
    17101 catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac
    17161 taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc
    17221 agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca
    17281 gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc
    17341 tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga
    17401 tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc
    17461 caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa
    17521 gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca
    17581 ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat
    17641 ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga
    17701 cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt
    17761 aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac
    17821 gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat
    17881 tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca
    17941 agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga
    18001 acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa
    18061 acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt
    18121 cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc
    18181 gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt
    18241 agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga
    18301 tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct
    18361 accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat
    18421 ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat
    18481 ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc
    18541 cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat
    18601 caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat
    18661 tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct
    18721 tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac
    18781 gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta
    18841 tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat
    18901 tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg
    18961 gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta
    19021 tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg
    19081 acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa
    19141 aaacaataaa taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg
    19201 tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc
    19261 agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga
    19321 aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt
    19381 cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact
    19441 gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca
    19501 aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa
    19561 aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt
    19621 cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa
    19681 atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag
    19741 cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa
    19801 aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc
    19861 cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg
    19921 tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc
    19981 aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa
    20041 tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg
    20101 atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga
    20161 gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt
    20221 gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa
    20281 atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga
    20341 ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat
    20401 atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc
    20461 tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc
    20521 gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg
    20581 accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata
    20641 cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt
    20701 attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga
    20761 cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg
    20821 cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat
    20881 atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga
    20941 actttgtaga taaagatatg gaagattaca cgaattttct attagagaca gattatgctg
    21001 attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga
    21061 aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc
    21121 cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta
    21181 tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg
    21241 acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc
    21301 tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca
    21361 aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg
    21421 atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga
    21481 tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat
    21541 atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc
    21601 ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg
    21661 ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa
    21721 gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct
    21781 ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca
    21841 ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc
    21901 gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg
    21961 agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg
    22021 gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat
    22081 gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa
    22141 tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc
    22201 actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta
    22261 cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat
    22321 gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta
    22381 aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta
    22441 ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga
    22501 atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc
    22561 acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt
    22621 gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga
    22681 agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta
    22741 tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa
    22801 gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg
    22861 atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa
    22921 gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg
    22981 aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta
    23041 gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat
    23101 acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa
    23161 gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta
    23221 tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta
    23281 gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg
    23341 agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga
    23401 aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat
    23461 tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg
    23521 aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact
    23581 aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa
    23641 cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt
    23701 agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa
    23761 tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga
    23821 attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt
    23881 agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg
    23941 tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat
    24001 cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa
    24061 gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga
    24121 gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac
    24181 gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac
    24241 tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac
    24301 tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca
    24361 gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg
    24421 aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg
    24481 aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa
    24541 gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac
    24601 gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag
    24661 aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg
    24721 agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa
    24781 acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat
    24841 gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc
    24901 tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat
    24961 cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat
    25021 catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc
    25081 tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac
    25141 taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc
    25201 gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca
    25261 attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt
    25321 aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa
    25381 aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag
    25441 cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca
    25501 ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct
    25561 gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt
    25621 caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt
    25681 ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa
    25741 gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg
    25801 gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt
    25861 ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca
    25921 gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg
    25981 gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg
    26041 gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt
    26101 acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta
    26161 gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga
    26221 acggctggat attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg
    26281 atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact
    26341 acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa
    26401 gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt
    26461 acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa
    26521 acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt
    26581 acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg
    26641 tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga
    26701 acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg
    26761 acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat
    26821 actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt
    26881 agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt
    26941 tataattcat atttttttat tttttctttt acgatactta gtccagaagc taaaatttca
    27001 aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag
    27061 cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg
    27121 tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct
    27181 actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct
    27241 aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca
    27301 gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga
    27361 actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag
    27421 attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt
    27481 gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg
    27541 gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc
    27601 ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc
    27661 attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca
    27721 atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca
    27781 gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac
    27841 ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct
    27901 ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata
    27961 aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata
    28021 tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg agaaataaat
    28081 gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg
    28141 ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga
    28201 gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga
    28261 taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa
    28321 gaattaatag gcattttctc cactccttaa aattgtatac catacaattt taccattgat
    28381 tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat
    28441 tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt
    28501 ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct
    28561 gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat
    28621 tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat
    28681 ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca
    28741 aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca
    28801 aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg
    28861 actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa
    28921 agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat
    28981 gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt
    29041 aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc
    29101 acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat
    29161 gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa
    29221 ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa
    29281 acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa
    29341 gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact
    29401 tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg
    29461 tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca
    29521 atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta
    29581 cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat
    29641 ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat
    29701 ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag
    29761 tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag
    29821 cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt
    29881 ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc
    29941 aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat
    30001 gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat
    30061 tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc
    30121 gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc
    30181 tttcaaatga attaaaaata aattggtgga gag
//
LOCUS       DAZUKA010000017        29100 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000017 DAZUKA010000000
VERSION     DAZUKA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.017"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG8PN_000608"
     CDS             complement(<1..1031)
                     /locus_tag="ACG8PN_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4860788.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG8PN_000609"
     CDS             complement(1113..1802)
                     /locus_tag="ACG8PN_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4860789.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8PN_000610"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8PN_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4860790.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8PN_000611"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8PN_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4860791.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8PN_000612"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8PN_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4860792.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8PN_000613"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8PN_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4860793.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8PN_000614"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8PN_000614"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4860794.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8PN_000615"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8PN_000615"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4860795.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG8PN_000616"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG8PN_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG8PN_000617"
     CDS             complement(7636..8523)
                     /locus_tag="ACG8PN_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4860796.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8PN_000618"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8PN_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4860797.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8PN_000619"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8PN_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4860798.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8PN_000620"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8PN_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4860799.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8PN_000621"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8PN_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4860800.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG8PN_000622"
     CDS             complement(10454..10957)
                     /locus_tag="ACG8PN_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4860801.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG8PN_000623"
     CDS             11554..12519
                     /locus_tag="ACG8PN_000623"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4860802.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG8PN_000624"
     CDS             complement(12618..13790)
                     /locus_tag="ACG8PN_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860803.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG8PN_000625"
     CDS             complement(13777..14922)
                     /locus_tag="ACG8PN_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860804.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG8PN_000626"
     CDS             complement(14936..15868)
                     /locus_tag="ACG8PN_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860805.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG8PN_000627"
     CDS             15985..17064
                     /locus_tag="ACG8PN_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4860806.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACG8PN_000628"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACG8PN_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACG8PN_000629"
     CDS             complement(17789..18016)
                     /locus_tag="ACG8PN_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4860807.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACG8PN_000630"
     CDS             complement(18119..20707)
                     /locus_tag="ACG8PN_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4860808.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACG8PN_000631"
     CDS             complement(20727..21386)
                     /locus_tag="ACG8PN_000631"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4860809.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG8PN_000632"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG8PN_000632"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4860810.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACG8PN_000633"
     CDS             complement(22159..23025)
                     /locus_tag="ACG8PN_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4860811.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACG8PN_000634"
     CDS             23134..23775
                     /locus_tag="ACG8PN_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4860812.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG8PN_000635"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG8PN_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4860813.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACG8PN_000636"
     CDS             complement(25216..26115)
                     /locus_tag="ACG8PN_000636"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4860814.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACG8PN_000637"
     CDS             complement(26117..26914)
                     /locus_tag="ACG8PN_000637"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4860815.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACG8PN_000638"
     CDS             complement(26892..27596)
                     /locus_tag="ACG8PN_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4860816.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACG8PN_000639"
     CDS             27727..28302
                     /locus_tag="ACG8PN_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4860817.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACG8PN_000640"
     CDS             28420..29097
                     /locus_tag="ACG8PN_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4860818.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAZUKA010000018        27769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000018 DAZUKA010000000
VERSION     DAZUKA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.018"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..1166)
                     /locus_tag="ACG8PN_000641"
     CDS             complement(222..1166)
                     /locus_tag="ACG8PN_000641"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4860819.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1462..2406)
                     /locus_tag="ACG8PN_000642"
     CDS             complement(1462..2406)
                     /locus_tag="ACG8PN_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4860820.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2627..3841
                     /locus_tag="ACG8PN_000643"
     CDS             2627..3841
                     /locus_tag="ACG8PN_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4860821.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3863..5878
                     /locus_tag="ACG8PN_000644"
     CDS             3863..5878
                     /locus_tag="ACG8PN_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4860822.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5961..7334)
                     /locus_tag="ACG8PN_000645"
     CDS             complement(5961..7334)
                     /locus_tag="ACG8PN_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4860823.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7360..7644)
                     /locus_tag="ACG8PN_000646"
     CDS             complement(7360..7644)
                     /locus_tag="ACG8PN_000646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4860824.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7646..9661)
                     /locus_tag="ACG8PN_000647"
     CDS             complement(7646..9661)
                     /locus_tag="ACG8PN_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4860825.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9840..10145)
                     /locus_tag="ACG8PN_000648"
     CDS             complement(9840..10145)
                     /locus_tag="ACG8PN_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4860826.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10234..11265)
                     /locus_tag="ACG8PN_000649"
     CDS             complement(10234..11265)
                     /locus_tag="ACG8PN_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4860827.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11472..12347
                     /locus_tag="ACG8PN_000650"
     CDS             11472..12347
                     /locus_tag="ACG8PN_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4860828.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12499..13341)
                     /locus_tag="ACG8PN_000651"
     CDS             complement(12499..13341)
                     /locus_tag="ACG8PN_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4860829.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13433..15310)
                     /locus_tag="ACG8PN_000652"
     CDS             complement(13433..15310)
                     /locus_tag="ACG8PN_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4860830.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15307..16728)
                     /locus_tag="ACG8PN_000653"
     CDS             complement(15307..16728)
                     /locus_tag="ACG8PN_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4860831.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16731..20120)
                     /locus_tag="ACG8PN_000654"
     CDS             complement(16731..20120)
                     /locus_tag="ACG8PN_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4860832.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20377..21768
                     /locus_tag="ACG8PN_000655"
     CDS             20377..21768
                     /locus_tag="ACG8PN_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860833.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21841..22488)
                     /locus_tag="ACG8PN_000656"
     CDS             complement(21841..22488)
                     /locus_tag="ACG8PN_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4860834.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22496..23182)
                     /locus_tag="ACG8PN_000657"
     CDS             complement(22496..23182)
                     /locus_tag="ACG8PN_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860835.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23188..24261)
                     /locus_tag="ACG8PN_000658"
     CDS             complement(23188..24261)
                     /locus_tag="ACG8PN_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860836.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24483..25157
                     /locus_tag="ACG8PN_000659"
     CDS             24483..25157
                     /locus_tag="ACG8PN_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4860837.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25162..26502
                     /locus_tag="ACG8PN_000660"
     CDS             25162..26502
                     /locus_tag="ACG8PN_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4860838.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26651..27637)
                     /locus_tag="ACG8PN_000661"
     CDS             complement(26651..27637)
                     /locus_tag="ACG8PN_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4860839.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8101 a         5244 c         5037 g         9387 t
ORIGIN      
        1 aaggattttt taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca
       61 gattaaattt catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat
      121 tctatttatt tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct
      181 gttaagcagt tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat
      241 acttctctta aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat
      301 aattcaacaa cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata
      361 tctttttcgt cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta
      421 ttcaagatag cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt
      481 ttacgatcaa tgatctcata agcagcatta cggactttat cagcaatcac atctaattca
      541 tcttgggcaa tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt
      601 gtatgtgacc atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg
      661 ctgcgtgggt caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat
      721 gttgttccag taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat
      781 gtcaaaatat ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg
      841 atttctttta cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat
      901 tgaccaggtt tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct
      961 tcatagccac ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg
     1021 tccaatgctt ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag
     1081 attccttggt tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt
     1141 actactttac gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac
     1201 tttcatgaaa agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt
     1261 tattataatt tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt
     1321 ttacacctat atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa
     1381 aattcgctgc aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg
     1441 ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag tacatgttga
     1501 atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat
     1561 ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt
     1621 tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac
     1681 cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc
     1741 gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct
     1801 ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga
     1861 acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt
     1921 caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt
     1981 tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat
     2041 ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt
     2101 gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc
     2161 tatcctttct ccattaataa tttttaaaca tgtaccgcta tacatataat tgatttcaac
     2221 aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt
     2281 tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa
     2341 gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt
     2401 acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt
     2461 ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca
     2521 taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc
     2581 acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa
     2641 aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt
     2701 cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga
     2761 attattaacg actgctccct cttttatggt cactattttt gttttgatta gtagttttat
     2821 tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg
     2881 tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact
     2941 aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca
     3001 cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc
     3061 tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt
     3121 ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac
     3181 aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa
     3241 aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat
     3301 taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat
     3361 cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac
     3421 aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt
     3481 gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc
     3541 tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc
     3601 ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc
     3661 atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt
     3721 caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac
     3781 gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata
     3841 aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta
     3901 tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg
     3961 ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag
     4021 atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt
     4081 agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc
     4141 aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga
     4201 cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg
     4261 ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga
     4321 atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg
     4381 agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct
     4441 atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat
     4501 aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga
     4561 ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca
     4621 agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc
     4681 cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg
     4741 gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat
     4801 caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac
     4861 tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa
     4921 agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat
     4981 ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga tatttattga
     5041 ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc
     5101 ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa
     5161 ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta
     5221 cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt
     5281 gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca
     5341 atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt
     5401 caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac
     5461 gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata
     5521 cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag
     5581 cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt
     5641 gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga
     5701 aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga
     5761 taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta
     5821 tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa
     5881 caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca
     5941 atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg
     6001 gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag
     6061 gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta
     6121 tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc
     6181 agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca
     6241 gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga
     6301 atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag
     6361 ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct
     6421 aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt
     6481 tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca
     6541 gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat
     6601 ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat
     6661 acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg
     6721 tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta
     6781 tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca
     6841 ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt
     6901 gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct
     6961 tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc
     7021 atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact
     7081 actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca
     7141 aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca
     7201 aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca
     7261 agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc
     7321 aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt
     7381 cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc
     7441 atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat
     7501 atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc
     7561 aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact
     7621 ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt
     7681 cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg
     7741 ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca
     7801 aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag
     7861 acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc
     7921 caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt
     7981 cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt
     8041 tttttggttg atccgataat tggatcattt cttctgttaa taattcagat agcataggac
     8101 gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct
     8161 tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt
     8221 gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct
     8281 gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag
     8341 taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta
     8401 agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac
     8461 tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc
     8521 tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac
     8581 caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc
     8641 ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta
     8701 aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca
     8761 ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt
     8821 ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg
     8881 atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc
     8941 cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt
     9001 ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta
     9061 attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca
     9121 aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa
     9181 atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata
     9241 ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt
     9301 ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct
     9361 gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat
     9421 aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa
     9481 atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat
     9541 attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg
     9601 ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca
     9661 cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt
     9721 ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg
     9781 atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat
     9841 caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca
     9901 accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct
     9961 acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca
    10021 gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct
    10081 tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt
    10141 gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg
    10201 cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat
    10261 gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac
    10321 tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc
    10381 aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac
    10441 accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt
    10501 cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac
    10561 gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt
    10621 tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg
    10681 tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg
    10741 acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac
    10801 ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct
    10861 ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg
    10921 gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc
    10981 tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc
    11041 ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag
    11101 atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg
    11161 ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata
    11221 gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt
    11281 ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg
    11341 gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt
    11401 aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg
    11461 gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag
    11521 agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg
    11581 aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa
    11641 tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga
    11701 ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc
    11761 agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc
    11821 agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct
    11881 ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca
    11941 agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca
    12001 atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg
    12061 aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc
    12121 agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg
    12181 attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt
    12241 tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac
    12301 aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca
    12361 tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc
    12421 tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat
    12481 tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata
    12541 tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt
    12601 gttctcggat actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg
    12661 ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac
    12721 gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat
    12781 ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa
    12841 agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat
    12901 aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc
    12961 tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg
    13021 gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc
    13081 ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt
    13141 tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg
    13201 ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa
    13261 tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag
    13321 tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa
    13381 aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct
    13441 gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac
    13501 cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg
    13561 gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa
    13621 atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt
    13681 acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg
    13741 ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat
    13801 atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt
    13861 ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg
    13921 acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt
    13981 tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag
    14041 gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag
    14101 tgacagaata atttactggg tcgatttctt tatttccatc atataaggca taagcatacg
    14161 ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg
    14221 ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa
    14281 tctcatattg aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt
    14341 tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca
    14401 ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta
    14461 cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa
    14521 tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt
    14581 tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt
    14641 ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt
    14701 cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt
    14761 tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt
    14821 gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt
    14881 cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag
    14941 ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca
    15001 aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa
    15061 attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga
    15121 attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct
    15181 ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc
    15241 tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat
    15301 gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc
    15361 gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa
    15421 tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc
    15481 agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg
    15541 tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc
    15601 atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt
    15661 ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa
    15721 tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga
    15781 gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt
    15841 gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc
    15901 atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat
    15961 gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa
    16021 cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac
    16081 aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg
    16141 ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata
    16201 caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat
    16261 attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt
    16321 atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt
    16381 aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat
    16441 ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt
    16501 gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt
    16561 ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg
    16621 taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt
    16681 aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc
    16741 cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact
    16801 agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc
    16861 gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct
    16921 tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga
    16981 ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt
    17041 tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt
    17101 ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc
    17161 gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc
    17221 tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt
    17281 cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt
    17341 gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga
    17401 taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct
    17461 ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt
    17521 tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc
    17581 gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct
    17641 ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga
    17701 agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat
    17761 ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag
    17821 tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg
    17881 tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg
    17941 tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca
    18001 atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat
    18061 ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga
    18121 tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg
    18181 actccgggag ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt
    18241 ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt
    18301 tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc
    18361 tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt
    18421 gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga
    18481 tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat
    18541 tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat
    18601 gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg
    18661 ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct
    18721 tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt
    18781 gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac
    18841 tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat
    18901 gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc
    18961 atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt
    19021 gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta
    19081 ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata
    19141 ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga
    19201 ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata
    19261 tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt
    19321 tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata
    19381 tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt
    19441 gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg
    19501 gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat
    19561 ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg
    19621 ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct
    19681 ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt
    19741 gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg
    19801 tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct
    19861 tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt
    19921 tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc
    19981 gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt
    20041 gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt
    20101 ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat
    20161 agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt
    20221 attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat
    20281 gaagttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa
    20341 catatgatca ggttgttgga aggaaactat agaaagatgg aaaaaatcat tcgaattctg
    20401 tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa
    20461 tctacatttg taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt
    20521 caatcttcac aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat
    20581 gttctgatgg aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc
    20641 aatccgggta aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt
    20701 acacgtaagt tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt
    20761 gaccaaggtt ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc
    20821 tttttctttt taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag
    20881 atcaaagaaa ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca
    20941 tatgagcaat ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga
    21001 actccatttg aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa
    21061 gacttcttaa tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta
    21121 gtgaactatt ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc
    21181 agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa
    21241 aaaaacgaag aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc
    21301 ccatacgata cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac
    21361 cgccagatac gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg
    21421 aatcagaatc tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct
    21481 gaaattcacc attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga
    21541 catagtcaat atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct
    21601 gagacaactg ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg
    21661 atcgttacga atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt
    21721 gtgtcattcg caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa
    21781 cagctatgta taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt
    21841 ttattgtatt ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc
    21901 ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa
    21961 gtataggaaa ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa
    22021 ttccagccat ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg
    22081 ctgatcattt cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa
    22141 gtcgcagcac cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct
    22201 aatgaatttt aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata
    22261 agcatcttcc aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc
    22321 aacgatttct gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag
    22381 gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca
    22441 catctctcct ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt
    22501 ccttcttttt ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc
    22561 attgaatcat acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt
    22621 ctttaactag taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt
    22681 cgtctgctaa gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc
    22741 tttctccacc agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt
    22801 gcagcaagga atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt
    22861 caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta
    22921 tttctttaaa gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt
    22981 tgatcactat ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac
    23041 ttttacctga accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat
    23101 caattgcttt caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt
    23161 tcatcgttaa tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt
    23221 ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact
    23281 atatagcaac cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact
    23341 agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg
    23401 tgctactaaa gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa
    23461 aatagcagtc ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac
    23521 taagaacaaa aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat
    23581 actttcaatg aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga
    23641 aattttagct gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa
    23701 cggctgttgt ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga
    23761 aactgtgtaa taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc
    23821 aattttgatc gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat
    23881 ttcattcttt gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt
    23941 cgttgtgccg aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa
    24001 tccgattggt gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga
    24061 agctgcaaat acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc
    24121 gattgctttt ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac
    24181 atacgcaatc aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat
    24241 ttctttgata gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat
    24301 gtcacttgtt gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct
    24361 tatcaataaa ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt
    24421 atttaagcct tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga
    24481 aaatgattaa actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa
    24541 tactcgctgc aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt
    24601 tggaaagtgg agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat
    24661 atgaagtgtt agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg
    24721 caaaagatag tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa
    24781 caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc
    24841 taggtctctt tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca
    24901 atgaagtctt gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg
    24961 tttatttttt acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct
    25021 ggggatttga ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa
    25081 aacatctgcg aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata
    25141 tactgcaaag ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt
    25201 tttgcgacaa atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta
    25261 cagatattaa accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt
    25321 gattctcgaa cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac
    25381 ccgactcaaa cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat
    25441 gaccgtttta acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca
    25501 gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat
    25561 accatccaaa atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg
    25621 attacgaaaa aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat
    25681 acaaatcaaa tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt
    25741 gttttttgga ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct
    25801 attattcttt cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc
    25861 acaccgttaa cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact
    25921 attattgaag aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga
    25981 ttgacttcgg atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag
    26041 caaatgatca atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc
    26101 atcatagatg gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat
    26161 gaaaaaaaaa tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat
    26221 ccagaagata agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta
    26281 aagaatactg gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat
    26341 cgggaagatc aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca
    26401 aaacaggtaa tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga
    26461 gtcgatttta aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag
    26521 ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag
    26581 tagcttcttc ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt
    26641 tagcttacgc ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa
    26701 tatccaattc ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt
    26761 tatgaatccc aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt
    26821 tgattgggat agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat
    26881 ctgttactgg tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt
    26941 cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat
    27001 ttacatctaa tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat
    27061 cgatgtcttc agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat
    27121 cagttcctgg tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta
    27181 cgtccattgg atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc
    27241 taacagtatc ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta
    27301 ctgtgatgac aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt
    27361 ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc
    27421 ataaagaacg gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag
    27481 acaatccgtc aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat
    27541 ctcgttcata cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac
    27601 tatagcttcc gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga
    27661 tttatgattg gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca
    27721 aatatttaga ttggaatttg attaaaacaa tttaattgaa atttgaaaa
//
LOCUS       DAZUKA010000019        26283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000019 DAZUKA010000000
VERSION     DAZUKA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.019"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8PN_000662"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8PN_000662"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4860840.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG8PN_000663"
     CDS             complement(937..1518)
                     /locus_tag="ACG8PN_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4860841.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8PN_000664"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8PN_000664"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4860842.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8PN_000665"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8PN_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4860843.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8PN_000666"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8PN_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4860844.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8PN_000667"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8PN_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4860845.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8PN_000668"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8PN_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4860846.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8PN_000669"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8PN_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4860847.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8PN_000670"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8PN_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4860848.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8PN_000671"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8PN_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4860849.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8PN_000672"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8PN_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4860850.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8PN_000673"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8PN_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4860851.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8PN_000674"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8PN_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4860852.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8PN_000675"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8PN_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4860853.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8PN_000676"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8PN_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4860854.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8PN_000677"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8PN_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4860855.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8PN_000678"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8PN_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4860856.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8PN_000679"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8PN_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4860857.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8PN_000680"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8PN_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4860858.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8PN_000681"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8PN_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4860859.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8PN_000682"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8PN_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4860860.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG8PN_000683"
     CDS             13972..14157
                     /locus_tag="ACG8PN_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4860861.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8PN_000684"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8PN_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4860862.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8PN_000685"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8PN_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4860863.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8PN_000686"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8PN_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4860864.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8PN_000687"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8PN_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4860865.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8PN_000688"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8PN_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4860866.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8PN_000689"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8PN_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4860867.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG8PN_000690"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG8PN_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4860868.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG8PN_000691"
     CDS             18232..18879
                     /locus_tag="ACG8PN_000691"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4860869.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG8PN_000692"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG8PN_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4860870.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8PN_000693"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8PN_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4860871.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8PN_000694"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8PN_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4860872.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8PN_000695"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8PN_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4860873.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG8PN_000696"
     CDS             20317..21255
                     /locus_tag="ACG8PN_000696"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4860874.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8PN_000697"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8PN_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4860875.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG8PN_000698"
     CDS             21935..22774
                     /locus_tag="ACG8PN_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4860876.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG8PN_000699"
     CDS             22750..23619
                     /locus_tag="ACG8PN_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4860877.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG8PN_000700"
     CDS             23609..24406
                     /locus_tag="ACG8PN_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4860878.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG8PN_000701"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG8PN_000701"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4860879.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACG8PN_000702"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACG8PN_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8540 a         4834 c         5643 g         7266 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggt attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agt
//
LOCUS       DAZUKA010000020        25300 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000020 DAZUKA010000000
VERSION     DAZUKA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.020"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACG8PN_000703"
     CDS             <1..615
                     /locus_tag="ACG8PN_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4860880.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACG8PN_000704"
     CDS             612..1385
                     /locus_tag="ACG8PN_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4860881.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACG8PN_000705"
     CDS             1382..1780
                     /locus_tag="ACG8PN_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4860882.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACG8PN_000706"
     CDS             1965..2636
                     /locus_tag="ACG8PN_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860883.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACG8PN_000707"
     CDS             2695..3135
                     /locus_tag="ACG8PN_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860884.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACG8PN_000708"
     CDS             3147..3815
                     /locus_tag="ACG8PN_000708"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4860885.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACG8PN_000709"
     CDS             3879..4502
                     /locus_tag="ACG8PN_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4860886.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACG8PN_000710"
     CDS             4558..4944
                     /locus_tag="ACG8PN_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4860887.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACG8PN_000711"
     CDS             5157..6548
                     /locus_tag="ACG8PN_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4860888.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            6895..7542
                     /locus_tag="ACG8PN_000712"
     CDS             6895..7542
                     /locus_tag="ACG8PN_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4860889.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    7671..7887
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7935..10580
                     /locus_tag="ACG8PN_000713"
     CDS             7935..10580
                     /locus_tag="ACG8PN_000713"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4860890.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            10890..12203
                     /locus_tag="ACG8PN_000714"
     CDS             10890..12203
                     /locus_tag="ACG8PN_000714"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4860891.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            12193..12846
                     /locus_tag="ACG8PN_000715"
     CDS             12193..12846
                     /locus_tag="ACG8PN_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4860892.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            12901..13593
                     /gene="radC"
                     /locus_tag="ACG8PN_000716"
     CDS             12901..13593
                     /gene="radC"
                     /locus_tag="ACG8PN_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4860893.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            13743..13943
                     /locus_tag="ACG8PN_000717"
     CDS             13743..13943
                     /locus_tag="ACG8PN_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4860894.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14197..15876)
                     /locus_tag="ACG8PN_000718"
     CDS             complement(14197..15876)
                     /locus_tag="ACG8PN_000718"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4860895.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15878..16090)
                     /locus_tag="ACG8PN_000719"
     CDS             complement(15878..16090)
                     /locus_tag="ACG8PN_000719"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4860896.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16483..18213
                     /gene="efrA"
                     /locus_tag="ACG8PN_000720"
     CDS             16483..18213
                     /gene="efrA"
                     /locus_tag="ACG8PN_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4860897.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18210..19970
                     /gene="efrB"
                     /locus_tag="ACG8PN_000721"
     CDS             18210..19970
                     /gene="efrB"
                     /locus_tag="ACG8PN_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4860898.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            20065..20262
                     /locus_tag="ACG8PN_000722"
     CDS             20065..20262
                     /locus_tag="ACG8PN_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860899.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20415..20900
                     /locus_tag="ACG8PN_000723"
     CDS             20415..20900
                     /locus_tag="ACG8PN_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4860900.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(21004..21597)
                     /gene="clpP"
                     /locus_tag="ACG8PN_000724"
     CDS             complement(21004..21597)
                     /gene="clpP"
                     /locus_tag="ACG8PN_000724"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4860901.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21777..22304
                     /locus_tag="ACG8PN_000725"
     CDS             21777..22304
                     /locus_tag="ACG8PN_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860902.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22395..23132
                     /locus_tag="ACG8PN_000726"
     CDS             22395..23132
                     /locus_tag="ACG8PN_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4860903.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23136..24053
                     /gene="miaA"
                     /locus_tag="ACG8PN_000727"
     CDS             23136..24053
                     /gene="miaA"
                     /locus_tag="ACG8PN_000727"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4860904.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            24055..25284
                     /gene="hflX"
                     /locus_tag="ACG8PN_000728"
     CDS             24055..25284
                     /gene="hflX"
                     /locus_tag="ACG8PN_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4860905.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8148 a         4304 c         5529 g         7319 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa ccttgctccc
     6841 gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt cgttatgcga
     6901 gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata tttacgctac
     6961 ttgaagttgc tggatgactc aggtataagt agaatcaaat caaatgagtt cagcgaaatc
     7021 acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga gctaggcaga
     7081 agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat ccttaatact
     7141 aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc attgaaacac
     7201 aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa tgatccagca
     7261 ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt cgcggagata
     7321 gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata ttcacaatct
     7381 gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc gcctgacaga
     7441 gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga gttgcagacg
     7501 cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt gagtaggctt
     7561 attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg
     7621 gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga agcataacgc
     7681 tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg ctgaaaggtt
     7741 ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag tagagataga
     7801 cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc aatttaggtg
     7861 gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt tttagtttaa
     7921 aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat ccgcaagaag
     7981 ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg agcggagata
     8041 aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga aaacttcatt
     8101 tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag agaatgcaag
     8161 gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca caagcaaaag
     8221 tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt gaaaagttca
     8281 tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa cagtgggcta
     8341 aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat ggattatctg
     8401 aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat cgtggtgaat
     8461 atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa gttattcata
     8521 aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga tcaggcgtag
     8581 tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt gctgttcatc
     8641 cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct ttagtcaata
     8701 aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca ggtgtagtaa
     8761 aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat gatcttccac
     8821 gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa tatgctggaa
     8881 tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga
     8941 ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt gtcgttgttg
     9001 aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg
     9061 ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat caaacgtttt
     9121 tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg tggggacatc
     9181 aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg gaagcaccag
     9241 cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt agttcagctt
     9301 tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat aagcgatact
     9361 tccctactag cactttagta accggttatg acatcatttt cttctgggta agcagaatga
     9421 ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg attcatggat
     9481 tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga atcgatccga
     9541 tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg aatggttctg
     9601 caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg aactttatca
     9661 acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg accgtagaag
     9721 agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact cgtttgaatg
     9781 aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa gcaggtcgac
     9841 aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg agtaaagaag
     9901 tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta gtccatacac
     9961 ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa gaaatatggg
    10021 aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt gtacgtccag
    10081 agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg attcgagccg
    10141 ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg ttgctaatta
    10201 aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat atcgaacgct
    10261 tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat ctagcgatgt
    10321 cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc aatattgaag
    10381 aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc
    10441 aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag
    10501 agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa
    10561 gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta gctattacta
    10621 aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct tagcttcgat
    10681 tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact cttattatgt
    10741 aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga taaaatcgtt
    10801 cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt tctatttgtt
    10861 ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata gaatggatcc
    10921 atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct ttattagaag
    10981 tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca aacggaaaag
    11041 gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca gtaggtacat
    11101 ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc aaaagcattc
    11161 cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa caacttgatc
    11221 aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg gcatttgatt
    11281 attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc ggcttattgg
    11341 attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga cttgatcata
    11401 ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga atcatcaaac
    11461 aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc atccaacaaa
    11521 cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa ataaactatc
    11581 tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata ggaaagatcc
    11641 ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga gtagccatcg
    11701 aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa gatgttttga
    11761 agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac gagccattag
    11821 ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag aatttgaaaa
    11881 aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact aaaaatgtta
    11941 gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg acgacctttg
    12001 aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag agagtatcaa
    12061 tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca aatgaagacg
    12121 tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta ttaatacaat
    12181 taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt gctgttcgac
    12241 acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg ttttccttat
    12301 gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg ggcgaattat
    12361 catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga tgattcatac
    12421 caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa
    12481 ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag taaccaacga
    12541 gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga gacgattgtt
    12601 tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt agctaatcag
    12661 aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa tggtatttta
    12721 gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc accatcccaa
    12781 gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc tgcattgtta
    12841 aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg agatgttttt
    12901 ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga acgtatggaa
    12961 atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact gcgtaccggt
    13021 caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt tggaggactg
    13081 gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat aggacgcgtg
    13141 aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa
    13201 agagcggctc caccggttcg taccagctac gaattggccc agcaattgat tctggaaatg
    13261 aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa tcaagtgatc
    13321 ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc tagagaaatt
    13381 ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa tcatccttct
    13441 ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca aaagtgtgga
    13501 gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg atattttagt
    13561 ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc
    13621 aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc tgttgcacct
    13681 ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag gaggatatta
    13741 ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga tttatctcac
    13801 gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac ggattcaaaa
    13861 cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc ggcccacaag
    13921 ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta gacatcaatt
    13981 tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat ttctcttgtg
    14041 gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa gagccacctt
    14101 gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg ttttctctta
    14161 tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg gtgtcattac
    14221 cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc
    14281 agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac attctcttaa
    14341 agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc gcatgtagac
    14401 aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga tatccactgt
    14461 cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta aaacgacatt
    14521 accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa aatgcccagc
    14581 aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga tgaagcagtt
    14641 ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca tgcggaattc
    14701 tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt tttgttcttc
    14761 ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca cttctgcacc
    14821 agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag gaataggtga
    14881 acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag taccattcgc
    14941 gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga tcatcgtttc
    15001 attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg gaactttgat
    15061 atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga aaacagcgat
    15121 tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga tgttagaagc
    15181 aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc
    15241 aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat cagataaaag
    15301 gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg gttcgccaac
    15361 aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag gtgttttcac
    15421 cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga cagaagtctt
    15481 tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc gaagcaagcc
    15541 atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat agataggaat
    15601 atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat
    15661 caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg gaatgacata
    15721 gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca aaatgatttc
    15781 atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac caatagcaaa
    15841 gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact aatgagaaat
    15901 cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg aattcaatat
    15961 tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt gcctcaatgt
    16021 aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga taataaactt
    16081 tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct agttgatttt
    16141 tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt tgactagaat
    16201 actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga tattttttca
    16261 accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca taaaacagca
    16321 aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat cccgctataa
    16381 tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg ctatcatttt
    16441 tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact aatgtggcat
    16501 tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt tggttttatt
    16561 ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg aatccgcaat
    16621 gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac tgtaatcggt
    16681 attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa tatcgtagca
    16741 gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga atacgaaaat
    16801 ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat catgttgttc
    16861 ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt cagtttgtac
    16921 atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt acctttacta
    16981 ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa gcaacaaaaa
    17041 aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg tgtgattcgt
    17101 gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga gaattatgct
    17161 tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt ttatttctta
    17221 tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga tgcaggcgag
    17281 cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt gttctcattc
    17341 atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc tggacgtatc
    17401 caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat atctgataca
    17461 aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg ccattcgcaa
    17521 gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt tgcttttatt
    17581 ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt ttatgatgtg
    17641 agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct aagcaagttg
    17701 cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac gattgcagat
    17761 aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc agctgagatt
    17821 gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca tttatcagaa
    17881 ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag agccatcata
    17941 cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta tcaaacggat
    18001 gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt gatcgtagca
    18061 caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga aggagaagtc
    18121 gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta tgatattgcc
    18181 gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc ttaaaacgtt
    18241 tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta tttacagttc
    18301 tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact acagaaatca
    18361 gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa tgtctcattt
    18421 ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt gttttgatga
    18481 caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa aaaatcaatc
    18541 gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct cgtgtgacga
    18601 atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc gtaaatgcga
    18661 tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg atggcgctga
    18721 tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc aatgcttcgc
    18781 agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac gtccaagaaa
    18841 atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca ttggaaggat
    18901 tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc atttctggct
    18961 tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg gctgttctag
    19021 gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca ttcatccaat
    19081 atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca gttttgcaaa
    19141 gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa gaaaaagaaa
    19201 acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc gatcatgtcg
    19261 attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa gtgaaagctg
    19321 gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg atcaatctat
    19381 tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc gacaccaaat
    19441 caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat gcatggcttt
    19501 atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca gattatgaag
    19561 tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg ccagatggat
    19621 atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa cagttgctga
    19681 cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa gcaacaagtt
    19741 cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg atggagggac
    19801 ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat ctgattctag
    19861 tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg gcacaaggtg
    19921 gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa gtcatataag
    19981 aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta aagtaaagtt
    20041 atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat tactgaagcc
    20101 aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga ttcagctgat
    20161 ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt gtacgaatta
    20221 attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt aaaacgtcag
    20281 ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt tttttcttat
    20341 gaaataaata tatgctatga tacctttatt caataggatt agtttctaca aaaaatgaaa
    20401 ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt ggtgatgtga
    20461 cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac agtaattgga
    20521 taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc gatcatgatg
    20581 cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta catggctgta
    20641 caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat gcggttattg
    20701 gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt attcctccta
    20761 atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa gaagaaatac
    20821 aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat gcgcaaggaa
    20881 aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg gtcagtttga
    20941 gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt cctttgaaac
    21001 agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat atgcttttgc
    21061 ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt caagcggttg
    21121 tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa tatggcgtgc
    21181 cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat ggatcatgat
    21241 ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca agaagcttcc
    21301 cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa agttcattgt
    21361 gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat aaagatagat
    21421 atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg aattagcaag
    21481 atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac gtgagtagat
    21541 atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta aattcataat
    21601 agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc taatacctaa
    21661 aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca aattagtcgc
    21721 ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac aacagattga
    21781 tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc tatatcggca
    21841 tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa ttggaattga
    21901 aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag atcttgcaaa
    21961 aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat tgttatatca
    22021 cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt tatctgccaa
    22081 attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt ttatctccag
    22141 atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt ttagagaatt
    22201 ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag actatgacgc
    22261 gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt gaagcattgc
    22321 cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa tagaactgaa
    22381 cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc acccacccag
    22441 aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt atcgaactag
    22501 atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa gtggaccgga
    22561 cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa aaattgaatg
    22621 cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg agagaagtgt
    22681 taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt aaaactgatc
    22741 agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt agagattggc
    22801 cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc gcattactgg
    22861 aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc caactagcag
    22921 cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc gaacatccag
    22981 tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat gaagaagaaa
    23041 tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg gagaaagctc
    23101 ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta gtgatcgtcg
    23161 gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta tttggaggag
    23221 agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt acagcaaaaa
    23281 tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc gaacctactc
    23341 aacgttactc agccgctgat tttcagcatt caggtcgaca attgattacc gagattgcga
    23401 accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa tcccttctat
    23461 atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa aagtatgaaa
    23521 cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa aaagacccgg
    23581 cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct ctggaagttt
    23641 tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa ttgtatgatt
    23701 acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc aatcaaagag
    23761 tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat gagcttggag
    23821 aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt gaagggaaag
    23881 aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac gcgaaacgtc
    23941 agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata agtgagccaa
    24001 atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa taaaatggaa
    24061 gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt ttcagagtca
    24121 atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt cacaattaca
    24181 caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt acaagaatta
    24241 gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga gctaactccg
    24301 agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg cgtgcagctt
    24361 atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca ggttgaactt
    24421 gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt atctcgtctt
    24481 ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga ccgccgacat
    24541 atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc tcatcgagag
    24601 cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat tggctacacg
    24661 aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta ttcgaaagat
    24721 caattgtttg caacgttaga tccattgact aagagatggc gttttgctga aggatttgag
    24781 attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact gatcgatgct
    24841 ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt agtcgatgct
    24901 agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga tgaactgaat
    24961 atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga cccagcgatg
    25021 ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga aggtaagatg
    25081 gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta tacactttct
    25141 gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat ggttttggac
    25201 gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa aaccaattca
    25261 aaatggttaa gaaaaaacca ataaaatttt atttttttaa
//
LOCUS       DAZUKA010000021        24927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000021 DAZUKA010000000
VERSION     DAZUKA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.021"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..475)
                     /locus_tag="ACG8PN_000729"
     CDS             complement(<1..475)
                     /locus_tag="ACG8PN_000729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860906.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNAD"
     gene            complement(531..2096)
                     /locus_tag="ACG8PN_000730"
     CDS             complement(531..2096)
                     /locus_tag="ACG8PN_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4860907.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(2179..2322)
                     /locus_tag="ACG8PN_000731"
     CDS             complement(2179..2322)
                     /locus_tag="ACG8PN_000731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860908.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(2319..2495)
                     /locus_tag="ACG8PN_000732"
     CDS             complement(2319..2495)
                     /locus_tag="ACG8PN_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFS4860909.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(2479..2673)
                     /locus_tag="ACG8PN_000733"
     CDS             complement(2479..2673)
                     /locus_tag="ACG8PN_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860910.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(2675..3052)
                     /locus_tag="ACG8PN_000734"
     CDS             complement(2675..3052)
                     /locus_tag="ACG8PN_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFS4860911.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(3126..4361)
                     /locus_tag="ACG8PN_000735"
     CDS             complement(3126..4361)
                     /locus_tag="ACG8PN_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS4860912.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(4361..5143)
                     /locus_tag="ACG8PN_000736"
     CDS             complement(4361..5143)
                     /locus_tag="ACG8PN_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFS4860913.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(5158..5730)
                     /locus_tag="ACG8PN_000737"
     CDS             complement(5158..5730)
                     /locus_tag="ACG8PN_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4860914.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(5864..6229)
                     /locus_tag="ACG8PN_000738"
     CDS             complement(5864..6229)
                     /locus_tag="ACG8PN_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4860915.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(6397..6825)
                     /locus_tag="ACG8PN_000739"
     CDS             complement(6397..6825)
                     /locus_tag="ACG8PN_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFS4860916.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(6822..8174)
                     /locus_tag="ACG8PN_000740"
     CDS             complement(6822..8174)
                     /locus_tag="ACG8PN_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFS4860917.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(8174..8458)
                     /locus_tag="ACG8PN_000741"
     CDS             complement(8174..8458)
                     /locus_tag="ACG8PN_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFS4860918.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(8455..8664)
                     /locus_tag="ACG8PN_000742"
     CDS             complement(8455..8664)
                     /locus_tag="ACG8PN_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860919.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(8802..11045)
                     /locus_tag="ACG8PN_000743"
     CDS             complement(8802..11045)
                     /locus_tag="ACG8PN_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFS4860920.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(11038..11382)
                     /locus_tag="ACG8PN_000744"
     CDS             complement(11038..11382)
                     /locus_tag="ACG8PN_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFS4860921.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(11375..12145)
                     /locus_tag="ACG8PN_000745"
     CDS             complement(11375..12145)
                     /locus_tag="ACG8PN_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFS4860922.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(12356..14293)
                     /locus_tag="ACG8PN_000746"
     CDS             complement(12356..14293)
                     /locus_tag="ACG8PN_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFS4860923.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     STHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(14354..14896)
                     /locus_tag="ACG8PN_000747"
     CDS             complement(14354..14896)
                     /locus_tag="ACG8PN_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFS4860924.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(14898..16040)
                     /locus_tag="ACG8PN_000748"
     CDS             complement(14898..16040)
                     /locus_tag="ACG8PN_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFS4860925.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(16041..16355)
                     /locus_tag="ACG8PN_000749"
     CDS             complement(16041..16355)
                     /locus_tag="ACG8PN_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFS4860926.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(16360..16566)
                     /locus_tag="ACG8PN_000750"
     CDS             complement(16360..16566)
                     /locus_tag="ACG8PN_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860927.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(16663..17280)
                     /locus_tag="ACG8PN_000751"
     CDS             complement(16663..17280)
                     /locus_tag="ACG8PN_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860928.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            17675..18625
                     /locus_tag="ACG8PN_000752"
     CDS             17675..18625
                     /locus_tag="ACG8PN_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFS4860929.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            18618..19064
                     /locus_tag="ACG8PN_000753"
     CDS             18618..19064
                     /locus_tag="ACG8PN_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFS4860930.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            19067..20899
                     /locus_tag="ACG8PN_000754"
     CDS             19067..20899
                     /locus_tag="ACG8PN_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4860931.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            20915..21118
                     /locus_tag="ACG8PN_000755"
     CDS             20915..21118
                     /locus_tag="ACG8PN_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4860932.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(21169..22305)
                     /locus_tag="ACG8PN_000756"
     CDS             complement(21169..22305)
                     /locus_tag="ACG8PN_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4860933.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(22295..24250)
                     /locus_tag="ACG8PN_000757"
     CDS             complement(22295..24250)
                     /locus_tag="ACG8PN_000757"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4860934.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(24243..>24927)
                     /locus_tag="ACG8PN_000758"
     CDS             complement(24243..>24927)
                     /locus_tag="ACG8PN_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFS4860935.1"
                     /translation="GLINGDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRK
                     ETRALAEKPWSFGEVRYDETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYN
                     APMWLLGLLESRMHMVWLRSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMT
                     EILDLREYEGGTLADLYNKDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLK
                     LYQEMTIND"
BASE COUNT         6868 a         5739 c         4609 g         7711 t
ORIGIN      
        1 tatcagcgtt agttgatgaa ccatatcgtt tatctcttac atcagaataa aataaatcag
       61 gaaagtcatg atacggccct ttacctttaa caccattgag agaaggtttt gatacatata
      121 gtggcataaa atttgcaaga gtatgactta aatcatagaa ttttacaaat ttcactttca
      181 tctcttcggt gatatcatcg gaatataaaa tgactttcaa acgcccataa tgactatctg
      241 gcattgaaaa gttcttttga ttatagcatt tcaataaccg tatcgttgca ccagcaattg
      301 tgttgaaaga aataatcgtt tctcccctat aattcccaat tccttcagca tactttgtca
      361 aatcttcagt tagaattttt ccccaccatt ttctataaaa cagtctagaa attaatgtgt
      421 tgtccgggtc tgaagatatt ttatctggtg ctacttgaat caaatattca ttcatacaca
      481 tacacctcca aagacaatta tatcaatgga ttgtaataaa agctcatatt tcacagaaac
      541 aacaatcccc tatcatcata aacactggca ccattatcat ttccacaacg aattgcacga
      601 tccagagcca taattgtcgc gatggcacca tcaatcttct cagtggattt ttctttatca
      661 gctttgatat tacctgccgg gtcagttcgg acgaaaatgt tgtccatatt ccaacgaaga
      721 accggatgac cgccatgggc taatttctgt tcaagagtca acttcatcaa ctctttagta
      781 gccgggctca tatccttgaa accttgaccg aaaggaacta cagtaaatcc cataccttct
      841 aagttttgaa ccatttgaac tgctccccaa cggtcaaagg caatctcccg gatattgtat
      901 ttctcaccca gttcctcaat aaatttttca atgaaaccat agtggactac gtttccctca
      961 gtggtttgta tgtacccctg cttggcccac aaatcataat tcacatggtc gcgtttaact
     1021 cgtaagtcca aggtatcttc tggcacccag taatatggca gtatcacaaa cttgtcatcc
     1081 tcatcttcag gtggaaacac caaaacgaag gatgtaaggt cggtcgtgct ggaaaggtcg
     1141 agacctccgt aacaaactcg acctctcaaa ctttcttcat ctgcattgaa tgaacaagcg
     1201 tcccatttat ccattggcat ccaacgcact gcttgtttca cccattggtt caggcgaagc
     1261 tgtctgaatg agttttcttc agctggattt tgtttagccg aatcacaagc agccaccacc
     1321 ttttcttctg caatcgttat cccaagtgac ggattggctt tcttccaaac ttctggatca
     1381 gtccaatcat catcttgctt gataccgtaa attaacggat agaatgtttt atcatgcttt
     1441 cgaccttcaa taatgtcgag agctttttga tgctgctcat aacaaattga attggtatca
     1501 ttccctgccg ttgtaatcaa aaagaatagc ggttgcgttc tagcatcccc tgcccctttc
     1561 gtcatgacat caaacagctt tctgttaggt tgtgcgtgaa gctcatcaaa tacaaccgca
     1621 gaactgttta atccgtgttt agagtatgct tcagctgata atacttgata gaaactattc
     1681 gttggctgat aaataattcg tttttgtgaa gtcaggattt tcactcgttt gcttaacgct
     1741 gggttctggc gaatcatatc aacagctacg tcaaatacga ttgaagcctg cgctcgatca
     1801 gcagcacagc catatacctc agcgcgttct tccccatcac cgcaagtcaa aagcaaagcc
     1861 acggcagccg ctaactcact cttaccattc ttcttaggta tttcaacata agccgtattg
     1921 aattgtctgt agccatttgc cttaatcgtt ccgaataagt cacggataat cttttcttgc
     1981 caaggcatca gtttgaacgg tttcccgaac cactgacctt tcgtgtgttt caacaactca
     2041 ataaaggcca cggcataatc tgcacgtgac ttatcataat gggagtcttt gtccataaat
     2101 tcagttggtt tgtaattatc gattggaact cactcccttc tacgcaaaaa gcagctgttg
     2161 tcagctgcct taatctcttt attccatcat atcggcaatg aactgaatgg cttcagggtt
     2221 gttcttgtta tcccgcagca attgcaccag ttcttgccaa tcgtagtatc cttcttcaat
     2281 gccatcttca ttatctggca tgtaaatttg ataatccatc atttatcacc ctccaagata
     2341 aagcggctgt actcatcttt gtgttcttca atgaaaatca ccaactcgta aaagtgccgg
     2401 tcatgtgcaa ttcgctgcac tgcaatcgca tcaaacatat ttgtcagacc actttctgca
     2461 atctcaatga tttgttgctt aatctttgcg ttcattttga ccgtcctttc tgcctgcctc
     2521 ataggccgca attaaggcat cttccaaaca ccaaaccgca atgtccaaga agtcttcacg
     2581 gtcgctgttg tgggccttca ggtcgccacg ttcaggcaac tgaacgatgt aattctttgc
     2641 gatgttgtaa atcttacgtt tactcacttt catcttaaag cccctttctg atgtagatgg
     2701 tatcttctgt ttttgactca aggtcaaccc agaccaaatc gtggttcaat atcatgtccg
     2761 tcacgtattg ccaagcatca ttgaaggttt caaatccttc tcttctaccg ttttgaaaga
     2821 ccaattcgaa gtctggtttg atgatgtgca ccacatcttc gccgtatacc gcgttcagtg
     2881 ttgaaccgtt gtcccattgg ataaggattg aaccaatgtc gtcaacaccg gttactgtgc
     2941 cttgtgtgcc cttcgctggg gcatgtgggt cgttcatctt gatgagttct acacgactac
     3001 cttcagggta acgttttttc ataccctcga tttgttcatg agttggaaac ataactttgt
     3061 cttccgtatt cttttggtag tctatatatc actctaaagg gattttatag caagtgatac
     3121 gttatttatt caggattttc taggtcatta aatgcgatag tctttccatc tcgaatcact
     3181 gaaacgtctg catcgcttcc agcctgctcg atataacgct taacaatcac atcacagaac
     3241 ttttcatcca gttcgatcgt atggcaaatc cggtcggtct gttcgcaagc aatcaaagtt
     3301 gatccgctgc caccaaaact atccaacacg atgcagttgc tgagtgacga attggtaatc
     3361 ggatatgcca acagcggaat tggtttcatc gttgggtggt caccattctt ctttggttta
     3421 tcaaattccc agatggtcga ttccttacgt ccggtgtacc attgatgttt gcctttcttc
     3481 ttccagccat agagcaccgg ctcatgctgc cattggtaag gcgaacgacc aagcactaga
     3541 ctctgtttct tccagatgca cgtacctgat aaatagaacc ctgcatccac gaatgctttt
     3601 ctgaagttca agccttccgt atccgcatgg aaaacataaa tactggcgtc gttggccatc
     3661 acactttcca tacaagaaaa ggcatcatac aagaagttgt aaaatgcctc gtccttcaga
     3721 ttatcatttt taatcgttcc cgctttacct tgatatgaga ccccataagg tggatctgtc
     3781 accacaagat ttgctagttt gccgtccatg agtttctcat aagtttcagg ttttgttgag
     3841 tcaccacaaa ctaaacggtg acgaccaagc aaccacaagt cccctgtttt actgaaggct
     3901 ggcttttcaa gttcctcatc tacatcaaag tcatcatctt gggcatcatc attaccgttg
     3961 agtaatttat caagttcatc agtttcaaaa ccaagcaaat ccaaatcaaa ctcttcacct
     4021 tgcaggtctg ataactcaac cgccaacatt tcatcatccc aaccggcgtt cattgctagt
     4081 cggttatcag caagtatgta cgcacgcttt tgtgcctcgg ttaggtgttc cacaaacacg
     4141 cacggtacct gcttgatacc ttcttcttta gccgcaagaa tacgtccgtg acctgcaatc
     4201 aagttataat ccttatcaac aatcaccgga tttatgaacc caaactcacg catagaagct
     4261 tgaatctgtt tcacttgatc cttactgtgg gtacgggcat ttctggcata agggaccagc
     4321 ttatcaatat caactttctc aaaacgttct gttgtgttca ttagaacaac ccccattctg
     4381 ctagtttctc aaacccgcca agctcattca cataatcacg cgcaacttcc acgatttcag
     4441 aataaggcac gccatcaatc tcagtgtcac caatcgcaca gcttaactca acgggcatac
     4501 cagtttcttg agctttcaag aatgcgtaga tgttcactga cacatcagct tttgataagt
     4561 ctttcccatg gataccgcca cctgttactc cgtctgccat atcggaacca agcttgcgat
     4621 tagtcgcacc tgtatccaca tccgggccgc cagtccaatc acctagaggg ttgactgtag
     4681 cgtttggata tttttgtttt aactcatttg tctttgcgtg gctctgacaa attatcaagc
     4741 gttcaccatc aagaatgtac tttccatcaa atggatggtc ctgatataag tcacgcgcaa
     4801 tttttgagag ctgtaactgt tcatcagtca atggcatccc acgaaagatt ccattatcgc
     4861 cacaacgaat ttcagtcgct tgattgtcag ccaactcttt atcttggggc acaatcacca
     4921 catctttttt aattttgcct gcgatacgat taatcgcttg gctgatttca ttcaaattta
     4981 aagcagttga agtttcaata atcacatgac agatgccatg accaattaaa acctcaactg
     5041 ctatctttgg attattttct tctgcgtatg ccaagtccac aatggctccg gcaatacgat
     5101 ctgcaacttt atctggatgt tgcggattaa ccttctctat cataaattct gttcctttca
     5161 accattctta gaacgtaaca atcgttccat cgcatccgta ttcggatctc ctgtgtattc
     5221 cactgatgca ttctctttta caatctgttg aatctcatac cacgtcacat ttgcttgctt
     5281 ttggaagttc tgcgacatcg ccacaaatgg ggaggccatg actgccttgg tagtcgggtg
     5341 ttttccgagc aaaccatatt gactcagagc attttcacat tgaacaaaac gcgcaaagtt
     5401 ctgagcgtag gactcaacca ttcgtggggc tatcaacgtt tcgcatccgc ggtctttcaa
     5461 ccattggaac gttgagttat aaatttcatc agcgcctagc acagtgccat ccttctgttt
     5521 tgcagaaagg taagcacttg gttcaggata atcattgccc tcaagctcta caccctcacc
     5581 aatatcttca ccaattaata attcaacatc cggtaagtca aattccatcc gcgatgcttt
     5641 ctttccagca gcaactttat caacgagtgg ctgtgagcgt tcccctgaac gaggtcgtct
     5701 gccaccgcgt tgtgttccat cttttgccaa aatttatcac ctctttttct tgtttggggc
     5761 tatcccccgt ttgaactgaa cttttttcgc gtaagggcct agcgcgctgt acagtttaga
     5821 agggtttagg gataattacc gcccctcccc cccttgttat ttttcacatg attgttttat
     5881 cttttgtgtt tcgtcgagtc atgtgaattt tcgagtgaca tgatttgcag acactcatca
     5941 aattattttc atcatgcgta ccaccatcac ttaattcctt gatgtgatgc acaatctcaa
     6001 ccgaagtaaa gcgtccttcc ttcagacaca tctcacatag gggatgtgct gccacgtacc
     6061 ttgcccgtat tttcttccac gctctgccgt aacgtttgtt gattgccgga tcacgctcgt
     6121 atttctcgta acgtttcctc gcaagtgttt tatgttcatc acaataagta tcatgcgtac
     6181 ggttcggaca acctgggaac ttacaggggt tagctggttt cattggcaag tggggtcaca
     6241 tcctttcggg caaaagaaaa gcccttgaag gatttttccc acaaaggctt cgagtatttg
     6301 ttcgatactt ctattgctgg tattgcttga gtatcgtttg tcttttttga catcttaatg
     6361 atatcactaa aggctactga cattcactga cacatttcaa atgactatgg gttcatctgg
     6421 tagcttcaag ttctcaagag ctttgctatg ccaacgaaag actgtccgca cgtcagcgtg
     6481 aagatcttca gcaatcttgt cccagctctc ttccaacagg tatcgattga ttaacacaat
     6541 cttttcgtcc acgtttgtga gttcatcaat cacgccacgc atttgtcgtt tgaggtctac
     6601 aaacttatca atctctgcat caatttcagc ttccaattgg taacacttct caaggttacg
     6661 aacaaatggt gcctcagtat tccgtgtgcc acttgaaatc ttttcttcga atgttgtgga
     6721 agaaactgtc accgccagtt gatagagctc cgccttctct ttaatccggt cgtcaattcg
     6781 tttattcaaa cggtacgctt gtttcaagta ttcttttggt gtcacttgct tccctccaat
     6841 ctagctttca ctgcatcaat caaacatgat tgtgttttat cttttcgttt caaagccaac
     6901 atcacatctt catcaatcgt atctttcgca ataatgtgat gaatgaccac agtatcactt
     6961 tgcccttgtc gccacagcct tgcattggtt tgttggtaaa gttccaaact ccacgtaaga
     7021 ccaaaccata caagtgttga accaccagct tgtaaattca atccatgtcc tgcactagcc
     7081 ggatgaatga cagcgatggg gatgctacct tcgttccaat caataatgtc ctgagacgtt
     7141 ttgatctgtc gaacgttgaa tcgttccttg atacgctcca gatcatgctg gaaccaatag
     7201 gcaatcagaa tcggtttgcc atttgtacct tcaatcaaat cttccaaagc atctaacttc
     7261 tggtcatgga tgtgatgact cacattaaac tcatcataga ttgctccatt cgccatttgt
     7321 agcagtttat tcgaaagcac cgctgtattg gctgcatcaa tggtggcttc tttcaattgt
     7381 agcaccaagt ctgccttaat ttcatcgtac ttttgctttt ctttgagtcc catatcaact
     7441 ggcacttcat tcatcacaag ctctggcata tccaagaaat cgactgactt cattgaaatc
     7501 gtgatatctg aaatcttgtc gtagatttct tgttctgcat cttcccttgg tttccaagaa
     7561 taaatctgca tgccattttg cttgtcaggt agaaagtatt tatctcgata acgtgaaata
     7621 taatagccca atctctcgcc catatccaga accttgaact ccgcaaacaa atccatcaaa
     7681 ccattacttg aaggtgttcc ggtcaatcca acaattcggt caaccttgtg tctgactttc
     7741 ataaagctag tgaaccgttt agcttttaag tttttgaagg acgacagttc gtcaatcacc
     7801 accatatcaa aatcaaacgt aaaacccgat ttgttaatca gccagtccaa gttctcacga
     7861 ttgatcagat agatgttggc attattttgt aatgctgcca ttcgctgact tggtgttcca
     7921 agaaccacgg aatacgataa atctttcaaa tgatcccact tttgaatttc agttggccat
     7981 gttgattgcg tcacgcgcaa cggtgcaata attaagacac gttgtattgt aaagtcatca
     8041 tgcatcaagt ctttaatagc tgtcagcgtt gtcaccgttt tacccaagcc catgtccaat
     8101 agaagggcgg cagttttatg atctttgata aaatcaatcg catatttctg gtagttatgt
     8161 ggtacgaact tcatcagaca tcacctccta ttctttctag taactcaggt atctgatcaa
     8221 tctcatcaag cacgaaacac tgaaacccta aatctgtcag ttgtttcata cgaaaaagct
     8281 gtaaagaccg tggcttcttc cctggcgcct tcacttcgac aaatccaagt ttcccatttg
     8341 gcataagtac caatcggtca ggcacaccag caagccccgg tgatgtaaac ttcgggcaaa
     8401 taccacctac ccttttgacg gctttcacta gtgcttgctc aacctgcttt tctctcatag
     8461 ccacaccgtc aaatactcga aatacgcatc acgcaagatt tccaagtatt ccttttctgc
     8521 gcagtgaatc atcagatgtg cgacttgctt atcgaatgat ttcttccaag gatacgtgac
     8581 atccgacatg gcaaactcgt aaaactcatc aaatcgtaaa tcacgtttac cgattccaat
     8641 ttgtaaccac tgtttaaatt tcatttagat attctccaat ctgcaagtat ggtgtaaccc
     8701 gtgaatacgc aatacataac tttatatagg tggtaatttt ttatccctta agaaaagttc
     8761 tatataagac ctacacaggt tacactttcc tttaaaatca actattccaa gaactcggat
     8821 ttgagttgca aaccaacaat cttgtttcct ttattagttc gccggcgctt aaatccgccc
     8881 gcctccagag ccgtgtaaaa atcggctgaa ctacgaatgt actcaccagt tcgcgtacaa
     8941 aacgcacgat actctgcata caactcacca gatttttctt caaagtcaac agcagtctcg
     9001 cagcactcgt ctaagaaatg gccaagccaa tcattcgccg tcttatattc agaaattgca
     9061 ttagcgacaa cttgtggaac cgttaaatga aagtctgcgg cgatgacttt tttcgcaccg
     9121 tcaaggatcc attgcaaaat ggctccgccg gcattttcaa aaaggtagtc cgcataattc
     9181 ttgatgtcct tattgccgtc aatcgtagca aggaatggaa tcacaattaa acgacgccag
     9241 gttcctttat caatcgctcc aacttttggg aggtggttgg tatagagcac caaagtatgc
     9301 gttgggacat acttgaatgg gtctttgtat ttcttctcag ccgcaatttc atccgtcgag
     9361 cagagctgct taatgttaga tgtgttgagt cgcatcccct cttcaagctc ggctgcgatg
     9421 agtaggcgtt tccccttcgc ttccgcaagt tcaggtttca cattgcgtcg gatttgactt
     9481 gtaagaatat cagctgagat gctgccagag taattaccga gcacacggct gatgacattc
     9541 cagaacgtgg acttcccgtt acgcccttca ccatacgaga tgataagcgc ttcaacgtaa
     9601 actttaccaa tagctgcaag tcctacaatc atctggacat aatcaatcaa ctcttggtca
     9661 cctacgaaaa tggtgttcag agcgtccaac caaatctgct gattggcgtc actcggggca
     9721 cattccgttt gcttcgtgat gtaatcttct gagttgtggt cttgtgattt gcctgttcgc
     9781 aaatcaaagg ttgctgttgg tgtgttcagc aagaactcgt tcacatctag taaccgttgc
     9841 tcgatttcaa gcatgggctt tgcctcggtt aatgctgacg agagatactt gctgtcgcgg
     9901 cgtttgacag caaacttctt atactcttgt gcgtgttggt acaaatcaaa actatgacgt
     9961 tgcgctgtgt tgaacatggt gactgccttt ttcggaccaa cactagcaag tacatcgaac
    10021 gcgccattac taaccatttc cttggtacgc ttctcaattt cggtttcaga ttcttccagt
    10081 tgacgttctg tcaatgcgtg gatcacacct tgcgcttttg gtttcgactc ttcccaatag
    10141 ctgccgttgt agacaaggta accagttgac ggtgagtaat gaagcttaca ttcatactcg
    10201 cgagctaaga ccgttgcttg acctaagtcc gagtagtcgg ttggctcaag tgataaatca
    10261 gagttgtact gctctggagg aatgtagcct tcttgttgtt tcactttctt cccaaagttg
    10321 accgcgctat tccagatagc tttaagctcc tcctccggca gtggcggatt acatttttgg
    10381 gattgttcga gatacttgtg atacgtatct tccgttgcac cgtagcgttt gatgagcttt
    10441 cctgcgatgt gactcatgct tgaattacgg ctgccttcgc taatctctga ctgcgcatta
    10501 tcccaatcag caaacagatc atcttcgaga aaatcagtga tcaacttact accttcatag
    10561 atttcaactt cagagtttgg tgttccgaaa agcaaacgtg ccgcatccat cgcattgttg
    10621 tcaaagtatg ggaactcact ttgaatctgc ttcttgagtt ccacatattc atctttatca
    10681 gttgtggcag gaatcgggaa atatacgtga aaacgtggac gtgctgattt gccacctttg
    10741 tctttcatgt tgttacgtga atagctgacc gcaaacgtca catcaggaaa ctgcatcgcc
    10801 acttcgaaag gggctaccca atcatctgga ttgtctgagt ggtcattgtc gcaatcaagt
    10861 ggaatcacat cagactggat gaagttgtca cctttgcgat agttgccttt gtactcagcc
    10921 atcacatgat ccagcttgac cgtggcaaca agtgtttctt tatctgtcac cacttgcttg
    10981 gttgggaaca atccattctt ggaattaccc acctgattgg atgtgaataa cgtcatctta
    11041 agcatttgag acctccttca tctcatcatc gaagtacctg atatgtttct tccaatactc
    11101 cgcttgttca atttcaactg ccatgccttg ggagatggtg tcacccacca cccagacttc
    11161 ctcacatttg cccatgagaa caagtgacat gtgcattgcc acatcacgct ccgttgcgtc
    11221 gtccatgaat tgtggataca tcagatgtgc cgcaactggc atgtagccac ggtcaagagc
    11281 aatacggcag taacgcttag tgtttttatg gttgacgaca acatttcctg agaacggcga
    11341 gcagacatag actgatgttc tgtaatcttt acgtctaggc attattatca cctcgctcaa
    11401 tcactggtaa gatgttcttc ttcgatttca gcaagtcgta caagaacaaa cgacctttct
    11461 gcgtccagta ggtatgcatc acgctgcgct ctgcatctat cgcatgagtt tttgattgtg
    11521 tgtagccttg attggcatac ttctgataaa gcaaccacat cttgccttgc ttgtactgaa
    11581 ttcccagttc atgaagcaac ttgttcattg cctgtggtga cataccgtag tccttcgaga
    11641 ttttagaaat cgacatcaca gaattgtttt gaagaatcaa atcgtagtag ctaacttttg
    11701 gtttcatctc caaaatcatt tgttgttgca tcgccaccat ttcttttttc tcttgtagct
    11761 gcttcaacac tgaaatggcc catgctggat cttcgattga ctttgtgatg aaatcttctg
    11821 tcgcgtacac accgtgttgg cggatagacg gcaagacctc atttgtcacc caacgcttga
    11881 atttcttcgc gttcggcatc ttgctagaaa tgatgagtga gtacaatcca ctttcgttga
    11941 taaggatagc cattgtccca ttaacgctga acgattcgtt caccgtctta tcttcctcat
    12001 caacatgatc tcgtaatgct ttttgtggat ttgtgtagcc aagaattgat gctacatctt
    12061 taccaacaaa gtgagggaca tcgccaatca tcaccgtgcg tacagagcca aactccgcat
    12121 tttcaaaaac tttcaattcg ttcatcttgt gaacctcctg taatagaatt ttcaggagag
    12181 gcattttgcc ccttctaact tacaggcgaa gaaaatggtc gaaagtaaac tctgactcga
    12241 atttattttt gatttttttg ccttcgtcta taagcgaaga aagaacccaa ttcgaacctt
    12301 atttttgata aaaaaatttg aaaaaaatat ggagccattt ctgactccac attgactaat
    12361 ccttttgata gaattcacac tcgtatccat cagcgttcag tagcaagcct tcagcccact
    12421 taggcgtttg actcatcaac tgacagatgt cttcaacctt cgattccatt ggcacttcga
    12481 taatcacttc gtcgtggacg tgcccgacaa tgcgatactt acgaagattc tccattgcga
    12541 aacagagaat gtctctagct attgcctgca cgatgttttc gacaaacttg ggaccatagc
    12601 tttcaagacg ttcccatttc ttggtagcac cgacaccttc ataggtaaca gcttcaccgc
    12661 cgaatttgtt ctcaccaatc tttggtttta cataaactaa tttacgtcct gacaagagtt
    12721 ggatgaacag aaaacccgac ttgtattcaa acttgatgcc atgcgtcgat gtgctccgtc
    12781 gttctttcac acaagtcttt actgccttat caacagccca ccaaagctcc gttatgttaa
    12841 tgtttgagtc tcgccaagaa ttaacaagcg gctgtaattc atcctcagtt agtcccattt
    12901 caagagcatc cattgctttc aatgcaccca ccgatcctcc gtaccctaga gcaagctcag
    12961 caattttccc tttttgacgt agatgcccat tcacgccatg cttttcaact ggcacaccga
    13021 acatctgact ggcagacgca cagtaaatat cgccaccatt tttgaatact tcttgacgcc
    13081 acttctcacc cgacagccaa gcaatcacac gtgcttcaat cgctgagaag tcagatacga
    13141 taaatttcat cccttcacgt ggtacaaacg ctgtccggag gagttctgag agtacgttcg
    13201 ggacattgcc gtagagaatg ttaagcattt caaaattgct ttgttttact aaactacgcg
    13261 cttcagataa atctgacata tggttctgag gcaagttttg aagctggatg ccacgtcctg
    13321 caaagcgtcc ggtacggttg gctccataga attggaataa accacgagaa cgattatcag
    13381 aacacgcaaa gttttgcatg gcttgatact ttttcacgga tgacttggcg agttgctgac
    13441 gtaagaccag gacttgcttt aaattttcat caataggttg ttcgagtagt tcggccactg
    13501 cctttttacc gagtgactcg gtttcaagtc cgtggtctag taaccatccc cgcatttgaa
    13561 gcacgctgtt tggattttct agtccagtta attcttgcaa ttgttgattc aatccagctt
    13621 ttgataattc atccaactga acggcagctt ccacaaagtc cttatcaatc tcaattcctc
    13681 ggtcgttgat ttcctgatcc agccagtatt ctttccaaac agaatcaagc actggaaaat
    13741 tggaaagttt tccttggata gctatttcaa cttccacatc acgactattg tatgtcttaa
    13801 atgctgacca tttagttggc gcatggtgtg gtaagttcct cgtgcgtcca ccattaactt
    13861 tggtcggatt gcatggcacg caaaagtatc ggatgaggtc ttttccttct gtgagctttt
    13921 gtttatccaa accaagaaca gcaccaactc ctacaagtga caacggcaaa cctaatgtcg
    13981 caccccacac cattgtgcat tgccaccctc gtggattcaa aaagccatcc aaccccagaa
    14041 agcgtgacaa acacacacgt tcaaaactcg cattgaaggc gtgtttaatc actgtttcat
    14101 ctgatagcga agttaatatt tcagccggga tagtttcgtc catcttcaga tcaaccactt
    14161 cgacgggacc accatctaca ctgtatgcaa atagcagtat ttcaaaatcg ggggactctg
    14221 aatatttgta gacaccacat ttcgataaat cgacactact atacgtttcc aaatccaatg
    14281 acaaatgttt cattttcttc ctccaatcaa acaggcgaca gcacttctgc catggccgtt
    14341 tttgttttaa tgattaagcc aagaaatcat catccgccaa ggtttcaaac tcatcgcttg
    14401 cagatacttg tgagccgagt ggttcgccgt cacgtacaaa ctggatattc ccaagccctg
    14461 ccgctacccc gcgatttcca tttgcgttaa aggcatagaa attaatcgac acacgagcgt
    14521 agcaaccact atacacttgt gaacgatcca agattggttg aacgtgctga tccacgattt
    14581 gtggtgcagt ggttgagttc gcattaatga agtaatggcc ttggtatgct tcatcgtcgc
    14641 gttcgatatc cccatcacgt aatggcagct tgatttctgc tttgtttggt ttcttcccac
    14701 cgaatttcgc aagtccctct tcgattgctg catcgattgc tttttcaatg gtatcaatcg
    14761 ttctcttatc tgattttgga atcaagactg ctgttgaata tttctctgcg cccccgttga
    14821 tggatgctgg ttcccaaccg tggaagtatg agagacgtgt gtttgtacca gtgattactt
    14881 tgcttgtttt agacatatta ttgttcctcc gttaatggtt taaattcttc gtttaagttg
    14941 tttactgtga ccgcttgtcg tttatctgaa ccatcgacta gcgtcagctt gccttttggt
    15001 ttgatgatgt attcgcccaa gatttcattg aatttctctt taccgagtgc cttcgtcatt
    15061 gctgtgacac caagtaactt tttatcatat gggtcgtatc cagccttgat gaccgccgca
    15121 gcgacatcat tgtcattaga atacttacga gtagagcgac cctcgactaa tttgtgatga
    15181 gtccattgtt tgccaccgag tgcaagtttc aaagcacagt ccttgatatc gttactccaa
    15241 gaaaccaggt catccacttt accgatgatt tcttcgattt catcatcact aagaagctct
    15301 ggtggtttga actcatattg cgccagtttc aagttatggt ctgctcgtgc acgacagcta
    15361 ttcttcgccg gacagaatcc acaccatttc ccgcattggt actcaccctc acctttgaaa
    15421 gctaaatctg cggcaggcga caacacatct tttgcccact gaagtaattc ttctttcagc
    15481 agtgtgtacg tactcacatt ctcacgcctt ggctggaaaa tggtcatctt gacttcttga
    15541 atgtcataaa taacatcgaa aagctccaac gctcccaagg cataacacat catctgtggg
    15601 ttctgatacg catctaccaa aattcctaga ccgtatttca tgtcaataac agatagggtt
    15661 ccatccgcaa tgacgagaca gtcgccggtc ccaaagccgt ctgggacgta ccttgagaag
    15721 tcgagccgct gttctactat tacaatagag tcaactgttg tctgtttcgc cttagcgact
    15781 tcttccatga cgaaggtcgc ataactttca gcacactgct ccatctcctc gtcgtagaat
    15841 tcaagcgtag atgttgggtc tgtgacgtca ttaccaatga acttattcag tttgtactca
    15901 cataaggtat gagcatcagt gccttgttgg gcatacgggc tcgacttgtt ttcaaagtgc
    15961 tctgacaagc gaacacttgg cggacagtga atccaccgat tacttgaaga tgcggatagg
    16021 actgcgtgtt gtgttggcat ctacaatccc tccgcatctg caaccaaagc cgcgtagttt
    16081 tctgtcggca catcactcaa gcgactggca ccatatttct caatcagtgc tttcacttca
    16141 gttgacttac cttcttgaga tttacgtgcc aacaaaccac ggacttcttc aagcgtcggt
    16201 tgtttgactt ctgattttgt ttccattgtc ggttctggtg tttcaacttg tggttcaggt
    16261 tcattggttt tcatcgccac agccaactct tgtaagctat cagctaacgc ttggacgtcc
    16321 tcaacgacct gaagaaatag ttttgtttta ctcacgtcat tacgctccct tcttgttaac
    16381 ttcacggatt tcgactgatt cgattgtgtc ccctggtgtg agcacgagaa ttttgtccat
    16441 gttgccctta ccgaacagcc accgtgcaaa ccagcgaggc aaatgacgca caccaccttg
    16501 taggacagtt tctttgttac ccgtgtcatc agtcacgttt actcggattt tgtgttttag
    16561 gttcatgatg gtcctccttc atctttattt gtttaggatc tactccctta cacaatacag
    16621 gcgaaggaaa acggggaaag taaactcccc cgtaactttt ttctaaaata attctttgat
    16681 cttgtctttg atgattccaa aggtgcgatt gattgactgt tttttcttgt caagcttgtc
    16741 cgccacttca gtctgagaca tgttttcagt gacgagcatg tgatagattt cttggtggta
    16801 cggtaataat tgcgtcacga cttcacgcaa acgatcgata cgaggatctt cttcattatc
    16861 ttggtaagca tcgtaaacca atgattggat ggggttgtcc ccctcatcgc attcatcatc
    16921 aaattcattg agcgacaaat tgtagttacg tttgaagtaa tcgttggctt caccatccac
    16981 gtattcgtcc attggataat agccgtgctc attattaaag tcttcacgga acttctcttt
    17041 ccaagccttg atcttttctt tttcttcttc tgtgcgttca ggacgtgcgt ttttataaaa
    17101 gttgtaaatc gcacggtcgt cagcgcgatg cagctcctga atgttaagct cagtgacgcc
    17161 gtcttcgcct gggcgaatct caaccacttc tttttggata cgtccattgg catcagtacg
    17221 ctcaaacacg tacttgtaag tgctgcggtc gttttggtgt gttttcataa atctactcat
    17281 tagatccctc tttcttgcct gaaagagctt ttgagcaagg aaaacaaaca gggaccgtgc
    17341 caatacgtac accgtccctc atcacgaaaa aagagcgcaa taaagtaagg catacaatat
    17401 ctgaaaaatt ttctggataa ccagttcttt tcagtatttg taaccttgcc ctattgcaac
    17461 tcaggcattg ttatatttta ttagtaggac tattcgcttt taagataaat tcgttcactt
    17521 tcttgaaaca taatgtagtg tttatgaacc ataaatgata aaattaaata gttaaactac
    17581 tatgttttaa ttatagaaaa aggcctccct tgttttcgtc agtcatcggc ggcaaacggc
    17641 ggtcaacggc ggacgtaaca aaggaggttg tattttggaa ataagtgaaa ttactcaaac
    17701 gctttatccg tttatgggaa gcgagtacgt gaagccacag gattttttaa ttgctttact
    17761 ggatagtgtt tcaaatgatg acgaggccac cattttcggt ttttctgatg ataacctacg
    17821 aagattttac aatggtactc gaccattaac acaagagtat gcagttcggg ttgctggaaa
    17881 attcaacatc gacaaatgca cgacttttat ttatgaacgt cttatagatg atgctccaac
    17941 cgtccttaag caacaattga ttgataaagg tgtggtaatc cccaaagata cagacgtttc
    18001 tgaaatagtg gcccatcttt tacttgatga aatcacctca attgcttctc aaacaaaagt
    18061 aaaaacaaaa aaggccaagc cgaagcttag cgaattagag ctcgcaagtt taagaatcaa
    18121 cactaatggt gatttctctt ataatgatga agtgaaaaaa ttctttgacg atttgccagt
    18181 tccccttgat attagagctg aggagatgac gtatgtcata gctctttttt cagcatatgc
    18241 agatgccgat ggatacgaag tatacgagca tacaaacgac ctgccaaatt cgcggctaaa
    18301 ggatttcaag agacagcgaa gaaattacta taaagctgaa agcatacgtc gtggggtccg
    18361 tgataacttt acacaagagg aaggaactga gcactttgaa gctttaaaag aagatatgta
    18421 cgacggcatc gaggaagtct atgaagaaga ctacgatgat ggcgttgaga gactcaaagc
    18481 cgttctacag cagtcgtctg ttataacatt aaacggatca atcttatcgt ttataccggg
    18541 attgctgcaa aattctgcta aaaaaggcat ttgccacatg ctggttaatg acggaaagat
    18601 caaatgggtg actgaagatg aatagtttgt ttaatactcc atttgaaatt tcggttcgag
    18661 taatgttact gctctctgaa tttaaagagc ctgtcagcgc tgatagaatt ttaatcacag
    18721 acttcatcac cacgtatggt cgcgactttg aaatttccga ttacaactta aatggtgata
    18781 acagctatag attcagtgaa tttattgcgc gacgtgaatt agttaaagat gcagtcaaaa
    18841 atcttgtact gcaacatgtt atccaaccat cgcaaagcaa agaaggtttt aagtatgctt
    18901 tatcagaaga tggcctaaag ttgatatcac aattcgtatc ggtatatgca gaaaaatact
    18961 tacaaatagc tgacgaatct gttaaataca ttcaagataa aagtgacgtt gagcttctgc
    19021 gacaaataaa ccaacgcgca aaggaattta aaggagagca atgataatgc aaaattatta
    19081 tattgaacgt gttaccataa aaggcaaaga tcgtgaagat gcaactgtca agttcaccaa
    19141 tggactaaac ataatttctg gcccttcaaa taccggaaaa actagcattg ttacggcaat
    19201 cgattttctc tatggcgcga aaaaagacgc tgctcccttt tcaaccgatg caaccggata
    19261 cagtaaagtt gaactgatta ttcaatcgac taatggcaaa tattcattaa ctagagaatt
    19321 tgatagcaac tccattttgg tatccaaaat aacgagttca ggtgaatatg tagattatga
    19381 aaaatataat gcacgatctg ggaaaaatcc aattagccat ttttggttac aacttttagg
    19441 catcgatgat gattatcaga ttatcaagaa tgaggatttc aaacgtcaaa aattaacttg
    19501 gcggacatta gcgcctagca cacttattcc acaagataga gtcaatacca gcgcctctgt
    19561 tctttttcct ggggaaacaa cccaacgcac agctttttta tcatcgttgt tacttctgct
    19621 atacggttca gattttgcga actcggaaga aaacgaaaca aaagaacata aaaaaattag
    19681 aaggaactcg gtgattaact acatcaattc aagcctttct gaattatatg aaaactacga
    19741 agtggctaaa tcagaattac ctgaggctcc ctctgatgat tttcaacaaa ttgtcgatgg
    19801 aatgttcgct gaacttgaaa caattgaaaa gcaaatcaat gatgcgttag ccagcagcaa
    19861 aatcttgata aaagagattt atcaaaagga acagtatctt catgaaactc aacttctttt
    19921 gaataggtac aaacagttac attatcaata tcaatcggat attgaacgac taacactaat
    19981 cgtagatggt gagcgattat taaatgatca taatgaacat gatacttcac atagctgtcc
    20041 attctgtaac ggcgaactaa gtaaacctgt ccaaacaacc tacatcgata ctgctaggtc
    20101 agaattacat tcattgctct ctcaattatc agatttgacg gaagaatctc atgataccga
    20161 agttgaactg caagagttaa tggaacagct tgatgagctt aaaacaagaa agaaatcgct
    20221 tgatgcagca atcaacgatg aactaaaacc gaaagctgag acgttaaagg atcgaatcaa
    20281 taaaatcgaa acttacaggg atattaaaca acagctaatc tcgatggaaa aaatgcaaaa
    20341 taagcttaaa agtgatcttg aaaaaatcga gaatgagcca gaagacgaac gtatccttta
    20401 taaacctaaa gagaaatttg atgcagaatt ctacaaagaa atgtctgagt tactggactc
    20461 catgttacgt aatatgcatt ataaacctaa tgaatttcgt aacgcatttt tcgccaagga
    20521 tattttcgac atcaaaatca atggaaatct aaaatggacc gaaaacggac atggatatac
    20581 agcgtttatc aattcagtag tggtaatggc atatcgacat ctcttgtata atcacgctaa
    20641 gtacacacct tctgtgttca ttatcgattc ccctcttctt ggattagatg aacgcaaaaa
    20701 acaagatggc gatgacaata tgaaagaagg acttttcaaa tactttatgg atcatcaaaa
    20761 cgaaggacaa cttatcatcg ttgagaatac aacgaacttg ccagcactaa actatgatga
    20821 taaacaagta aatcagattg tctttactga agataaaaac aatggacgct atggatatct
    20881 atacgatatt tataactaga acaggaatga ttacatgaag tttagctaca atccgctttg
    20941 gaaactccta atcgacagag gactgaataa agcacaactc agaaaaatga ctgatatcag
    21001 ttcagcaaca ctctcaaaac taaataaagg cgaaaatgta acaacagata tcttacttcg
    21061 catctgtgaa gcacttgatt gtgatttatc ggatattgtt gaaatcgttc gcgattaaat
    21121 atcaaaaatg cactgtattc gattcatgag tacagtgcat tttcatattt atttttgtaa
    21181 tccagcgatt acttcattga ttttcgattc aatatcattc aatggaacca tataccattc
    21241 agtagctttt ttgaagtttc catcctgaaa gacaacttgt ttgtcctgga atacatgatg
    21301 gagagtgcgc tcaagtagct gagcgttgac attttgtact tcagcagaaa gcatgacctt
    21361 cactggtgca tttaagtagg tcacgtcgtt ttccgcattg gcaattctac tttcaacatc
    21421 atttcgagtg aaaccaatct tatacaggtt attccacttg ctaatatccg gattcgtgct
    21481 taatgacttc agcacgtaga tataacctgt cgtaaactct tctccaaagc tttcagccag
    21541 tgtttcctca tctaccggct ctgtcaccat ccttccatga cgttcagtcg agtatagaga
    21601 cgaagctaaa gattgtaaca acagattctt attctctgtc ccattctcat aaacaacatg
    21661 cattctggca tttgttttcc cattatcagc gataaaggta tccttcgaaa tggaaacgat
    21721 atacatcatt acgccgtttt ggatgtagaa tctattttca tgaatatcat actgctcaaa
    21781 cgggataatt tttcgtctgc cagatgcgat atctgcatgc acttgatcga acaagctctt
    21841 atacttttca aaatgacttg catgttttct gcggccaatt ttatctgcag cgttcacagt
    21901 tctacgatag cgggaaacat ccaacaagtc tgaaaaatca tcaatcatat catctgagcc
    21961 taagatatca tctaaattca actcgtcatc gatttcaact gaatcagcga gaaactctcc
    22021 atccggttcg aattctttct ccaataaacc aagagtgtca tacttcttaa ccttctcaga
    22081 cctttcgggt tttcctcgaa atccgctaag acgagcccac aatgcgcgtt cactagacca
    22141 ttcaggagtt ttttctggct ctcgtccgtt ttcattcaca aagtctataa tctcctgaaa
    22201 tttagcaact tctgggtctt gttttacaac tttctttttt tcaacttgct tggttaaatc
    22261 atcaaaagcg tcatcttcaa agatgctatc taaactattc gccatcttct ttcgctgcct
    22321 ctctttcacg ttttatctca gcaagtcgta gcaaagcaaa ggccaagtat ttttcaccat
    22381 catctttact atttcgatca ggatttcgac cattttctgc acggaacttc ttagcagctt
    22441 ggtaggccgc aaccaattgt tcgtcagtaa aatcatactt atttgatgac aaataatcct
    22501 gaataaaccg aagagtcggt gcatcaatat cacgactaat cacttcatac gcacgttgga
    22561 acggattcac ttgatcgatt aaatcaatat tcaattcatc aatgttaatg aaacgatcgg
    22621 ccatcttaat aatttcgcgg ctaccatctt ctgaagttgt tgtccgtcca gccgcaatat
    22681 ttatatcggc aactgtatgt tgacgaacgg tttctaactc ttcgttcgtc aaatcaggat
    22741 aacgatcaat gatgacacgt ggaatcaacg tcttattaat catcttcgca tcagcacctg
    22801 aaacaatcgc attttgaata tcttggctct gtaaaatgct agatttcaaa tcttccatat
    22861 cattttcgat gattttcttt gtacgttccg tcgtatcagc aggtttcagt cctctgattg
    22921 aaagatcacc tggtttgtct ggcttaccat cttttggttt aaacttgtaa actggcgcca
    22981 acacttgttc cataagtaga gatgcagaaa ttgccttcat aatactgtta accgcgaata
    23041 atacttcgtc atcctgtgca tctggctgcg caataagatt tgtaaactgt gcatgagtct
    23101 tattggggct gtcacgagtt acgcgaccaa tgatttgaat cacttcagtt aatgagttac
    23161 gataaccgat tgtcagtgca tattccgcaa atggccaatc gaagccttct tttgccattc
    23221 caagtgcaat gatgatatca agcttatcaa gatctttaat gtcatccgta atacttgcta
    23281 gatatgctga gactttttca cgtccttctt cattcacaag gtcggccact aatagttttt
    23341 taccatctgg acgttcgatt tcataaatac catcctcgtt ctgtgtgata ttaccaccgt
    23401 ccgtcaataa atcaagtatt tcatcaactt ccgcgtattt atctttagta gactcaccag
    23461 aattaacatt aggaatatgg ataattgatt ttttgttgac atcaattact ttatccaaag
    23521 catctgtata ttttccacga tagaaactat accccattgc aaaggatttc agatatttat
    23581 atccttccag ttgctcataa taagtgtaag tgaccttatc aaatttatct tcgtcggctg
    23641 gttccagaat ctgaacagag tcgccacgga aatatgaacc agtcattgct aagatatgcg
    23701 cattagagtt tgacatgatg ctacgtaagg cattacctaa cacagattta tcatcacggc
    23761 tcacatgatg gaactcatca atcgctaata gcgtattgtc aaataatgaa tcgtccaact
    23821 cttcaaaagc aaatcgcaac gtagcatgtg tggcaatcag gatattatca cttgggtcag
    23881 tcgagttcat gaatttaaca aagtgttgaa cctttgattt ttcaccgccc ccaatagtta
    23941 agttgtcact tggctttact tcccaatccc aataaaaacc aaacttcgat aattcagtat
    24001 ctttaaatga tttaccaatt gagcgttcag gaactgcaat aaccactttt ttaattcctt
    24061 gattatggag tttatccaag ccaacaaaca tcaatgcacg tgattttcct gaagctggtg
    24121 gtgattttac caacaaatac tgtgacgcac gcttttcata aacacgttct tgcatttcac
    24181 gcataccgag gtcatttgtt gtttttgatt gatttttatc aaaattaatt tcaaataaat
    24241 tactagtcat tgattgtcat ctcctgatag agttttagta atgttccaag tcgatcttcg
    24301 tcagattcaa acgggcgttg ctgataagct ctatcaacaa tcccatccag ttcctggtgt
    24361 ttcttacgta atgattctgg catactgtct ttattataca aatcggctaa cgtgccgcct
    24421 tcgtattctc gtaaatcaag aatttctgtc atcacgcgag tcatttcatt tttacgctgg
    24481 gcggataaat cctgaatcgg aaaagtatta tagactaacc cggctgaata acggtaccga
    24541 gtttctaatt tcccaccgac agagcgcagc cacaccatat gcattcggga ttctaacaga
    24601 ccaagcagcc acattggagc gttatagatt gccatagccg aatctgaaat tatagtatcg
    24661 ctcccaacta tacccatagg aacataatct cttttttctg atgacacacg aggaattaga
    24721 atactattct cactggtttc atcataacga acttcaccaa atgaccatgg cttctcagct
    24781 agtgctcgag tttcttttct cttgcttttt tccctataac tttttacatt ttttattgtt
    24841 tctttaataa gagaattttg tttgtatcta gttgcatctt cctcggtcaa ccataaagta
    24901 tatcttaagt ctccatttat taatcct
//
LOCUS       DAZUKA010000022        24520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000022 DAZUKA010000000
VERSION     DAZUKA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.022"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..1104)
                     /locus_tag="ACG8PN_000759"
     CDS             complement(256..1104)
                     /locus_tag="ACG8PN_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4860936.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1328..3268)
                     /gene="thrS"
                     /locus_tag="ACG8PN_000760"
     CDS             complement(1328..3268)
                     /gene="thrS"
                     /locus_tag="ACG8PN_000760"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4860937.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3605..3784)
                     /locus_tag="ACG8PN_000761"
     CDS             complement(3605..3784)
                     /locus_tag="ACG8PN_000761"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4860938.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3831..5654)
                     /locus_tag="ACG8PN_000762"
     CDS             complement(3831..5654)
                     /locus_tag="ACG8PN_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4860939.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5932..8211
                     /gene="helD"
                     /locus_tag="ACG8PN_000763"
     CDS             5932..8211
                     /gene="helD"
                     /locus_tag="ACG8PN_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4860940.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8304..8951)
                     /locus_tag="ACG8PN_000764"
     CDS             complement(8304..8951)
                     /locus_tag="ACG8PN_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860941.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9130..9849)
                     /locus_tag="ACG8PN_000765"
     CDS             complement(9130..9849)
                     /locus_tag="ACG8PN_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4860942.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9980..11131)
                     /locus_tag="ACG8PN_000766"
     CDS             complement(9980..11131)
                     /locus_tag="ACG8PN_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4860943.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11348..12088)
                     /locus_tag="ACG8PN_000767"
     CDS             complement(11348..12088)
                     /locus_tag="ACG8PN_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4860944.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12092..12415)
                     /gene="rsfS"
                     /locus_tag="ACG8PN_000768"
     CDS             complement(12092..12415)
                     /gene="rsfS"
                     /locus_tag="ACG8PN_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4860945.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12472..13065)
                     /gene="yqeK"
                     /locus_tag="ACG8PN_000769"
     CDS             complement(12472..13065)
                     /gene="yqeK"
                     /locus_tag="ACG8PN_000769"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4860946.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13049..13699)
                     /locus_tag="ACG8PN_000770"
     CDS             complement(13049..13699)
                     /locus_tag="ACG8PN_000770"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4860947.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13709..14020)
                     /gene="yhbY"
                     /locus_tag="ACG8PN_000771"
     CDS             complement(13709..14020)
                     /gene="yhbY"
                     /locus_tag="ACG8PN_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4860948.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14036..15145)
                     /gene="yqeH"
                     /locus_tag="ACG8PN_000772"
     CDS             complement(14036..15145)
                     /gene="yqeH"
                     /locus_tag="ACG8PN_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4860949.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15142..15669)
                     /locus_tag="ACG8PN_000773"
     CDS             complement(15142..15669)
                     /locus_tag="ACG8PN_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4860950.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15849..16634)
                     /locus_tag="ACG8PN_000774"
     CDS             complement(15849..16634)
                     /locus_tag="ACG8PN_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4860951.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16627..17496)
                     /gene="accD"
                     /locus_tag="ACG8PN_000775"
     CDS             complement(16627..17496)
                     /gene="accD"
                     /locus_tag="ACG8PN_000775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4860952.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17497..18876)
                     /locus_tag="ACG8PN_000776"
     CDS             complement(17497..18876)
                     /locus_tag="ACG8PN_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4860953.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18884..19303)
                     /gene="fabZ"
                     /locus_tag="ACG8PN_000777"
     CDS             complement(18884..19303)
                     /gene="fabZ"
                     /locus_tag="ACG8PN_000777"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4860954.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19328..19804)
                     /gene="accB"
                     /locus_tag="ACG8PN_000778"
     CDS             complement(19328..19804)
                     /gene="accB"
                     /locus_tag="ACG8PN_000778"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4860955.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19807..21042)
                     /gene="fabF"
                     /locus_tag="ACG8PN_000779"
     CDS             complement(19807..21042)
                     /gene="fabF"
                     /locus_tag="ACG8PN_000779"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4860956.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21061..21798)
                     /gene="fabG"
                     /locus_tag="ACG8PN_000780"
     CDS             complement(21061..21798)
                     /gene="fabG"
                     /locus_tag="ACG8PN_000780"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4860957.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21810..22727)
                     /gene="fabD"
                     /locus_tag="ACG8PN_000781"
     CDS             complement(21810..22727)
                     /gene="fabD"
                     /locus_tag="ACG8PN_000781"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4860958.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22834..23058)
                     /locus_tag="ACG8PN_000782"
     CDS             complement(22834..23058)
                     /locus_tag="ACG8PN_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4860959.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23111..24073)
                     /locus_tag="ACG8PN_000783"
     CDS             complement(23111..24073)
                     /locus_tag="ACG8PN_000783"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4860960.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24083..>24520)
                     /locus_tag="ACG8PN_000784"
     CDS             complement(24083..>24520)
                     /locus_tag="ACG8PN_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4860961.1"
                     /translation="EPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIEA
                     IGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGKL
                     LFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6732 a         5444 c         4264 g         8080 t
ORIGIN      
        1 tacccaaaaa cttcttaata aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa
       61 tttcaagata aaatcaactt tttgtttcac agcaatcact taaagttaca tatccaacta
      121 tttttttcat taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca
      181 ctttgatgga tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa
      241 aaaatagtaa ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt
      301 tttcactttt tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat
      361 cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg
      421 tctagatgtt tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt
      481 caacgcaatg atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc
      541 acttctacct gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg
      601 cgggagcaag acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt
      661 ttgcagaata tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc
      721 tgccgattcc aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt
      781 ttcttttatc gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc
      841 atttgcttct atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt
      901 gattttgaag tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg
      961 cgcactcaac ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat
     1021 attaccgata atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact
     1081 ttcaatacga ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag
     1141 tataccatat cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc
     1201 gcccaacaga atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg
     1261 aggaatacct gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat
     1321 ttacctatta gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga
     1381 tattcaaatc ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat
     1441 cttccaattc cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac
     1501 gtatcttata gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt
     1561 gcaaacgttc tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga
     1621 ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat
     1681 aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg
     1741 tattttcccc gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta
     1801 attgtatagt tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt
     1861 ttggtccgta aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca
     1921 tagcggcttt cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat
     1981 ctgtattgtt tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag
     2041 catatactgc caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac
     2101 gaacaaatgt atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag
     2161 cacctgattt ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac
     2221 gataagaatg gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa
     2281 gaacaagcat ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat
     2341 cccagtgtcc agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt
     2401 ggtaacctag actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg
     2461 ccccttttgg aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt
     2521 caagttcttt tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat
     2581 attctttcaa agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat
     2641 tgtttgagtt tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac
     2701 ggccagtgga tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag
     2761 cagtaatcac ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg
     2821 caaaaatttc taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt
     2881 tgacgatttt catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt
     2941 gtccattatc tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga
     3001 tatttggata gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta
     3061 agccaagtgc atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg
     3121 gacgagtcaa atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc
     3181 tttttgaaat gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg
     3241 caccatctgg aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat
     3301 cagatcatca agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac
     3361 gaaaagttcg tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg
     3421 gtccgaccgc ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg
     3481 gaaaagtttc agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc
     3541 tcatcaatca attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact
     3601 aaacttattt tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga
     3661 tgacatggat gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt
     3721 tcatcatatt ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg
     3781 gcataatgct ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg
     3841 tttgtcttgg gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc
     3901 aaaatagaga ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt
     3961 cccgtacttg caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt
     4021 ttcgctattc tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca
     4081 tctttcaaga tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt
     4141 gcaataccga tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc
     4201 gcaacaggtc tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag
     4261 ctacgggcat gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat
     4321 ccatccccgg tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc
     4381 tttgctgctt ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca
     4441 attgttacat agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca
     4501 cgtccaatct ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct
     4561 ttcagttgac taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct
     4621 aagatatggc tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt
     4681 attccattga ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt
     4741 ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca
     4801 acgatcccgt ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga
     4861 atcagtggac aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag
     4921 aaccaccaag aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga
     4981 tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc
     5041 atttgatact ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca
     5101 tttaaagacc ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct
     5161 gtcaaagaag attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca
     5221 agttcgccag gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc
     5281 tgcaattttc cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc
     5341 agttctttac ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg
     5401 atcaatatca tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta
     5461 atcgctaata catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac
     5521 atcaagaagg cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg
     5581 tgaaaaacaa attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt
     5641 ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat
     5701 ttccagctgc ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg
     5761 ccttaattga aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa
     5821 aaatattcag caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa
     5881 actatgctat acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag
     5941 aaacaactag aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat
     6001 atgctggata caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca
     6061 gctaatcaca agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat
     6121 cgccaacatg agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc
     6181 ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa
     6241 gaccgtgaaa cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa
     6301 acaatcgtta ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt
     6361 cccgcatttt atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa
     6421 tttaaaatcc aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat
     6481 gactttttgc tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct
     6541 acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt
     6601 gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg
     6661 tatcataatc gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg
     6721 ttttctgatt acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag
     6781 acattcaaaa attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa
     6841 caagaaagtg gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg
     6901 accttagtcg atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc
     6961 cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat
     7021 aaagaaacaa atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc
     7081 ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg
     7141 gcggaagaac agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa
     7201 aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga
     7261 atccatcgag ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt
     7321 ctacaaactg tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag
     7381 gaacatttga caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct
     7441 gtactattct ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc
     7501 tatatcttta ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc
     7561 ttgttcccaa aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat
     7621 gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa
     7681 ttaacgacaa gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg
     7741 aaagaacatc aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga
     7801 acaacagatg atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa
     7861 gcccggtatt ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat
     7921 gctcaactgc cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt
     7981 atgaagcgtt ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga
     8041 gttttcttgt gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt
     8101 tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt
     8161 cctttgttaa acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga
     8221 caactgctat cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa
     8281 gtaattcaat catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat
     8341 tttaatttga caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg
     8401 tcaaatacct tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc
     8461 cattcaatga attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa
     8521 atctctgata cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct
     8581 atcttctcat ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat
     8641 agtagaaaga aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa
     8701 cgacgaaaaa gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc
     8761 accatacact ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag
     8821 ccttcaacgc cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca
     8881 gctaactctt ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt
     8941 tttgcttcca tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac
     9001 ttatgctctc atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca
     9061 agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa
     9121 actggttgct tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc
     9181 ttccagtgca tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc
     9241 gttttgaggg atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa
     9301 tgcttcttgt atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc
     9361 ttccattaca tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc
     9421 gtcttctcct gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga
     9481 aacacccatg ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt
     9541 cacattgttc gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata
     9601 gcgcaattca gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat
     9661 aatatgtttt ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt
     9721 tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc
     9781 ataaatccgg ctattattag catctttagc agttttcttt gctacgatat ttgcccattt
     9841 acgtcccata ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg
     9901 ttccattata cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt
     9961 ctcattttta gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa
    10021 tattgcctcc caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct
    10081 ctttttttcc gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc
    10141 cagcatcggt aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt
    10201 gctttttgat attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc
    10261 tttttgtttt tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt
    10321 tcatgcgcga ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag
    10381 aagataacaa ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag
    10441 ttgaaagatc ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta
    10501 acgcatggcg aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat
    10561 agcctgcact tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt
    10621 cctttgcata acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg
    10681 gatacagact gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca
    10741 gttcttgaaa tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc
    10801 tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt
    10861 gcaagatttt tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg
    10921 gaagctccac tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat
    10981 cgatgatagc cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct
    11041 ctgctcctgt tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat
    11101 tgtactctac gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa
    11161 aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa
    11221 ctattctcgc aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat
    11281 atcgttcgga tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc
    11341 aaaaccgcta gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt
    11401 cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta
    11461 aataactttc caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac
    11521 cagacccttc ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc
    11581 ttcctggata actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt
    11641 ctggaaaaac ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt
    11701 cttctaacgc ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat
    11761 agcacagcga atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga
    11821 gcatattgcc ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa
    11881 tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg
    11941 caaccgctaa tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta
    12001 aatgacgttt cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt
    12061 aaatgaaagc aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta
    12121 gcggagcatc tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa
    12181 catgtacgat cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa
    12241 tacgttttac ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt
    12301 tgatttgacg ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat
    12361 ttcgaacgtc taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa
    12421 tttttaattg ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt
    12481 tgctacccag taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag
    12541 caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc
    12601 aatggctcgt gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac
    12661 ataaatgatt ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc
    12721 atgaagaatc tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag
    12781 accatgccag attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat
    12841 taccattttg aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat
    12901 actagctttt tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac
    12961 tccaagcaca tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt
    13021 ttctctagaa taagcagtat attcattact atagtccata ttcatacaac ccttcatttt
    13081 gaatataatc aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct
    13141 tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat
    13201 atggagtatc cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca
    13261 gctcatcgat tttataccat tttggcaaat attctaccat atccccaccg ataataaagt
    13321 aataatctgt atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt
    13381 tcccaccacg agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta
    13441 attccaacat gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg
    13501 gctgataaga aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag
    13561 ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct
    13621 ttttcttctc taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct
    13681 tagcttgcgt attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt
    13741 gatacttttc ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga
    13801 tttcgcaatc tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat
    13861 tttgaagtaa cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga
    13921 tcattgctga attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt
    13981 tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa
    14041 tcaatgcttt gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg
    14101 gcgctgttac agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag
    14161 aaaactcaaa acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta
    14221 ataatccacc tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa
    14281 tatttacatc atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa
    14341 atcttgcaag tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt
    14401 tgatttcttt taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt
    14461 gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct
    14521 cgattttatc caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg
    14581 ctgtattttt gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga
    14641 tatagacatc ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat
    14701 gagatttctt agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac
    14761 gttccttcat ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt
    14821 tgttcccaac taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg
    14881 aaccattgaa gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt
    14941 cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat
    15001 ggcgtaatct gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag
    15061 ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc
    15121 cgatacattg taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt
    15181 tgctaataga taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact
    15241 atctgttgcg ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg
    15301 tatatcagtc ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg
    15361 tttctttgct atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag
    15421 atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac
    15481 agggattcct gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg
    15541 attattccat gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt
    15601 ctttaattgg gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt
    15661 tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac
    15721 cccgtattat actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa
    15781 aaagcatttc tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca
    15841 aaaaacaact aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct
    15901 aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta
    15961 tatcgtggca actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac
    16021 tcgcgtagtt ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg
    16081 tctttccaca aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc
    16141 agcatccaga cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca
    16201 atgatgatgg cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata
    16261 gcctctcctt cgccgcgctc ttctgctcct acaccgcaat aagcacctgc tgtattgatc
    16321 aaggtaatca ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga
    16381 tagccttcag gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct
    16441 ttttgaatac cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc
    16501 gcttcgtcat ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct
    16561 tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt
    16621 gctgtcttat ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat
    16681 tttctgtttc aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa
    16741 ttcagccttt tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct
    16801 tccagcaaaa ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa
    16861 actggcagtt actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc
    16921 attgctatgc cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc
    16981 ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt
    17041 tgctcgttcg aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat
    17101 aaaattagga tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt
    17161 gaggactgct tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc
    17221 gggaaatgct agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc
    17281 atcaataatc aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg
    17341 gcatactttt tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt
    17401 tgcccacatg ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt
    17461 gatccgaata tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc
    17521 tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat
    17581 gactgcagga tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt
    17641 gataaattca cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg
    17701 gacgattatt tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac
    17761 tgcactgtct actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc
    17821 tggagaagga gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg
    17881 tccttggaat actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac
    17941 ctgttttttt actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat
    18001 tcgagtattc atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt
    18061 tccggcattt tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc
    18121 tctttttgtt tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg
    18181 ttgtaaagaa caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat
    18241 ctgaacttct atatggcgag caggataaat gattttttcc aaatacatat catcatttcc
    18301 aaatgccgct gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga
    18361 ttgaactttc cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata
    18421 accaattctg tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc
    18481 tggaatgact gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat
    18541 tgcatcgatc gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga
    18601 ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt
    18661 cacgattgca gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg
    18721 gccaatgcag attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga
    18781 atatatcgct acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat
    18841 ttcaccgcgg ttagctatca atactttaga aaacatattt cacctatcca atcataaagg
    18901 ttaattccgc ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg
    18961 ctgaagcttt cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt
    19021 tcttacggaa ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt
    19081 caagagaaag taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg
    19141 gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt
    19201 tcttagcaac gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg
    19261 gataacgatg tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct
    19321 gctttgctta tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt
    19381 tttctacaag gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt
    19441 tcatcgcttc aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga
    19501 aagctggttt gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct
    19561 cgattccatc aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt
    19621 gttcaggtat gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat
    19681 tcatgcctcg acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat
    19741 cgaattccgt tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat
    19801 ccaaaattat tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa
    19861 tgaattactc attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa
    19921 gtcaatctca ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa
    19981 tgccagcaaa gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat
    20041 cgatttcgtg ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc
    20101 ttttgcttct gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac
    20161 ttgttccggc tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga
    20221 accatctgga gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat
    20281 ttctccatag attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac
    20341 gcctgctcct tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg
    20401 attaggatca gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc
    20461 acaaatcgtt gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt
    20521 acggaatgct tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca
    20581 aatacctttt gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat
    20641 tggtacgaac attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt
    20701 ttcgattgtt ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt
    20761 cattttttct ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata
    20821 gatcgaaaac atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa
    20881 atctttgact tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg
    20941 tgcgatgcca ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat
    21001 aggtgaagtg actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc
    21061 ttatccgtgc atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt
    21121 ttgtgctaag aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg
    21181 aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt
    21241 gatgaatcct ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac
    21301 agattttgtt aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt
    21361 tccaatcaac ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat
    21421 ttttttcaat acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt
    21481 tttgaaatct tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt
    21541 tactaacaca tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt
    21601 ctcataatca gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc
    21661 ttctatcttt tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt
    21721 tgcaaaagct aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt
    21781 ttttcctttt acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc
    21841 tgcttattct caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt
    21901 aatacttttc cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg
    21961 atactctcat agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt
    22021 tctttcttca ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat
    22081 tgaatctgac ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga
    22141 aaaggcccgc tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa
    22201 acagcttcgt ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa
    22261 tttgcaggag atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca
    22321 atcggcgcat tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata
    22381 taagcgcctc tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct
    22441 actaatgccg aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc
    22501 tccttcaata agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc
    22561 gtctgatcca aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc
    22621 tcacttgctt catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata
    22681 ccttgatatt gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat
    22741 atatccagaa tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag
    22801 gaaatgtccc agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt
    22861 cactaaatct tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc
    22921 ttcaatttca ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat
    22981 gtcagtagtc aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg
    23041 aattttttcg aataccataa taatatattc ctccgatatt gtttgctttt tatttttata
    23101 atttaacagt ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg
    23161 taaatattac tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca
    23221 ataagatagg gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact
    23281 tttcttgcgg catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat
    23341 gcaaaagcag ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt
    23401 ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg
    23461 aatcttctga atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc
    23521 tggtaccgtc agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga
    23581 gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta
    23641 gcttagatag tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt
    23701 tttcagccat gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga
    23761 tagcatgagt tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat
    23821 cgggcgacat tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt
    23881 ttatcaattt ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc
    23941 ttcgttcacg aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg
    24001 aaagatcatt attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc
    24061 caaattgttc cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca
    24121 gtgcatccag caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca
    24181 tttcacgatg aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca
    24241 actttacaac gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta
    24301 acttgttaat agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg
    24361 aagttgtttt tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca
    24421 gatctttgaa ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga
    24481 cactaacaag ataatcattt actgttttta aatctggttc
//
LOCUS       DAZUKA010000023        24259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000023 DAZUKA010000000
VERSION     DAZUKA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.023"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG8PN_000785"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG8PN_000785"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4860962.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG8PN_000786"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG8PN_000786"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4860963.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG8PN_000787"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG8PN_000787"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4860964.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACG8PN_000788"
     CDS             complement(3373..4680)
                     /locus_tag="ACG8PN_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4860965.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACG8PN_000789"
     CDS             complement(4683..5264)
                     /locus_tag="ACG8PN_000789"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4860966.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACG8PN_000790"
     CDS             complement(5836..7128)
                     /locus_tag="ACG8PN_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4860967.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACG8PN_000791"
     CDS             complement(7125..7748)
                     /locus_tag="ACG8PN_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860968.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACG8PN_000792"
     CDS             complement(7751..8587)
                     /locus_tag="ACG8PN_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860969.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACG8PN_000793"
     CDS             complement(8604..9491)
                     /locus_tag="ACG8PN_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4860970.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACG8PN_000794"
     CDS             complement(9507..10790)
                     /locus_tag="ACG8PN_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4860971.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACG8PN_000795"
     CDS             complement(11473..12156)
                     /locus_tag="ACG8PN_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4860972.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACG8PN_000796"
     CDS             complement(12174..12791)
                     /locus_tag="ACG8PN_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4860973.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACG8PN_000797"
     CDS             complement(12801..13625)
                     /locus_tag="ACG8PN_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4860974.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACG8PN_000798"
     CDS             complement(13618..14178)
                     /locus_tag="ACG8PN_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860975.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACG8PN_000799"
     CDS             14352..15308
                     /locus_tag="ACG8PN_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4860976.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACG8PN_000800"
     CDS             complement(15613..16569)
                     /locus_tag="ACG8PN_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4860977.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACG8PN_000801"
     CDS             16673..17275
                     /locus_tag="ACG8PN_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4860978.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACG8PN_000802"
     CDS             complement(17363..18862)
                     /locus_tag="ACG8PN_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4860979.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACG8PN_000803"
     CDS             complement(18878..19402)
                     /locus_tag="ACG8PN_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4860980.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACG8PN_000804"
     CDS             19700..20449
                     /locus_tag="ACG8PN_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4860981.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACG8PN_000805"
     CDS             20440..21225
                     /locus_tag="ACG8PN_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4860982.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACG8PN_000806"
     CDS             21194..21910
                     /locus_tag="ACG8PN_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4860983.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACG8PN_000807"
     CDS             complement(22012..23031)
                     /locus_tag="ACG8PN_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4860984.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACG8PN_000808"
     CDS             complement(23050..24051)
                     /locus_tag="ACG8PN_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4860985.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7624 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DAZUKA010000024        24254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000024 DAZUKA010000000
VERSION     DAZUKA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.024"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1141
                     /locus_tag="ACG8PN_000809"
     CDS             158..1141
                     /locus_tag="ACG8PN_000809"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4860986.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(1509..3647)
                     /locus_tag="ACG8PN_000810"
     CDS             complement(1509..3647)
                     /locus_tag="ACG8PN_000810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4860987.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(3686..5272)
                     /locus_tag="ACG8PN_000811"
     CDS             complement(3686..5272)
                     /locus_tag="ACG8PN_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4860988.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(5262..6482)
                     /locus_tag="ACG8PN_000812"
     CDS             complement(5262..6482)
                     /locus_tag="ACG8PN_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4860989.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(6494..7300)
                     /locus_tag="ACG8PN_000813"
     CDS             complement(6494..7300)
                     /locus_tag="ACG8PN_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4860990.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(7510..8790)
                     /locus_tag="ACG8PN_000814"
     CDS             complement(7510..8790)
                     /locus_tag="ACG8PN_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4860991.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(8834..10867)
                     /locus_tag="ACG8PN_000815"
     CDS             complement(8834..10867)
                     /locus_tag="ACG8PN_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4860992.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(10901..11752)
                     /gene="rfbD"
                     /locus_tag="ACG8PN_000816"
     CDS             complement(10901..11752)
                     /gene="rfbD"
                     /locus_tag="ACG8PN_000816"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4860993.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(11850..12878)
                     /gene="rfbB"
                     /locus_tag="ACG8PN_000817"
     CDS             complement(11850..12878)
                     /gene="rfbB"
                     /locus_tag="ACG8PN_000817"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4860994.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(12900..13472)
                     /gene="rfbC"
                     /locus_tag="ACG8PN_000818"
     CDS             complement(12900..13472)
                     /gene="rfbC"
                     /locus_tag="ACG8PN_000818"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4860995.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(13486..14352)
                     /gene="rfbA"
                     /locus_tag="ACG8PN_000819"
     CDS             complement(13486..14352)
                     /gene="rfbA"
                     /locus_tag="ACG8PN_000819"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4860996.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(14452..15159)
                     /locus_tag="ACG8PN_000820"
     CDS             complement(14452..15159)
                     /locus_tag="ACG8PN_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4860997.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(15164..15991)
                     /locus_tag="ACG8PN_000821"
     CDS             complement(15164..15991)
                     /locus_tag="ACG8PN_000821"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4860998.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(15991..16791)
                     /locus_tag="ACG8PN_000822"
     CDS             complement(15991..16791)
                     /locus_tag="ACG8PN_000822"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4860999.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(16784..17923)
                     /locus_tag="ACG8PN_000823"
     CDS             complement(16784..17923)
                     /locus_tag="ACG8PN_000823"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4861000.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(18128..19321)
                     /locus_tag="ACG8PN_000824"
     CDS             complement(18128..19321)
                     /locus_tag="ACG8PN_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861001.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(19400..19606)
                     /locus_tag="ACG8PN_000825"
     CDS             complement(19400..19606)
                     /locus_tag="ACG8PN_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4861002.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(19599..20741)
                     /locus_tag="ACG8PN_000826"
     CDS             complement(19599..20741)
                     /locus_tag="ACG8PN_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4861003.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            21253..21888
                     /locus_tag="ACG8PN_000827"
     CDS             21253..21888
                     /locus_tag="ACG8PN_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4861004.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            22024..22116
                     /locus_tag="ACG8PN_000828"
                     /pseudo
     CDS             22024..22116
                     /locus_tag="ACG8PN_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            22230..22865
                     /locus_tag="ACG8PN_000829"
     CDS             22230..22865
                     /locus_tag="ACG8PN_000829"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4861005.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            22972..24210
                     /gene="mef(H)"
                     /locus_tag="ACG8PN_000830"
                     /gene_synonym="mefH"
     CDS             22972..24210
                     /gene="mef(H)"
                     /locus_tag="ACG8PN_000830"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4861006.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT         7347 a         4901 c         4188 g         7818 t
ORIGIN      
        1 atccactcat aaagtgaacc tttatatttt tcagagaatc catcttccca aagcgctgtc
       61 tagtccataa gaatacctgt attacgtgaa gaatcgaaaa aattgaaatc gatttttaat
      121 gacattatgc ttatagaaga aagtgaggta atatcttatg aataaacaag agttgcatga
      181 ttttattggt tttcgacaac caaatatatg tcaaattgtc gcatataagg atggggtaga
      241 agtttataca gatgaatgga atggttataa aaaagacgat acttgtcata tcatgtcagc
      301 aacgaaaagt attgtatctt tgctgattgg cattgcttta gagcaaggat taatcaaaag
      361 tatcgatgat aaagtattag attattttcc agagtataaa gtgaaacgcg gggagaaaac
      421 tatttacgct attacaatca agcacttgct aacaatgaaa gcaccctaca aatgtaaagg
      481 cgatccatgg acaaaagtct gttcaagtga agattggaca aaaacatcac tagatttatt
      541 gggaggaaga aaaggtataa ccggcgaatt caactatcaa actgtctgtt tacacatatt
      601 aactggcttg ttgtatcaga caagtaagct aacaactgtt gattttgcca ataagtatct
      661 atttgatcct atagggatac caaaccatgt caattattat gctgaaacag ctgaagaaca
      721 taagcagttt acaatggata agtctcctaa aaccaatgtc tggttttgtg atccactaaa
      781 tatcgggacg gcaggatatg gtttgtgttt atcggcaagt gatctggcaa aaattggtgt
      841 attatgccta aataaaggga tgtataaagg aaaacaaatc gtaccaccca tgtggataaa
      901 cgagatgttc aaacctagaa ccagtgcttt agaagactta caaaatatga gatatggcta
      961 tttatggtgg gtattagatt ctgagaacga aatatattca gcaattggta atagtggaaa
     1021 tgttctttat attgacgcta agaacaatct ggttatctcc atcacttctt attttaaacc
     1081 catgataatt gatagagtgg cttttgtaga ggaatacctt aaaccattta ttttaaaata
     1141 gcctatatta aaataaaata tcgattcttt gacagctagg gttcgtgaaa ctttggctat
     1201 gattttgtat ctattggcgc tctgagcatg aataagagcg tgggacaaaa gtaagaaaca
     1261 cttttgtctc attctttaaa aacgaataaa cggtgggagc agaagcaact tcttcttaaa
     1321 taagccgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttctcgg
     1381 agttaaacac ttctgtccca acctcgactt atttttttga gtcggcacct tctgcctttt
     1441 tttgacaaaa aactataaaa agccgttatg tgcgagcgaa tcacaacata acagcttttt
     1501 attatagttt aatctggtct caacgaaaag tcgccgattc cttcttttgt taaattatca
     1561 ttgtaaaatg gatcatgcgc aatatatgct ggccatttat cttgcatttt tttgatttca
     1621 ccagcaaaac gtctttgttt ttctggagta tcttcataac ctcttgattt agattcgaaa
     1681 tggtacaatt ctgcttgatg tgcatagaca ttgtaacgac ccagttcata aactttcaag
     1741 cacaagtcaa catcgttgaa tgctacttct aatgtttcat caaacccgtt aacagcattg
     1801 aagtcagctg ccttgaccat catacaagct gcagtgactg ctaagtaatt gacgtcaatg
     1861 accaatcggc cgaaataacc acaatgtgtt cgatcatagt tattcaatgc atgccctgca
     1921 acaccaccga ttcctaataa tactcctgca tgctgcgttg tatcatctgg ataaaacagt
     1981 tttgctccta cacagccgat tcgatcaaac tgtgcataag aaaccatcac cgtcatccaa
     2041 tctggctcaa tcacttctgt atcgttattc aagaataaaa agtatttgcc gtttgctttt
     2101 tctgctgcta aattgttaat tcgagaataa ttgaatggaa tgtcaatcaa ttctacgata
     2161 aagcggtctt tcagctgatg cttgtattct gcaaataatt cttgcatttt tggatctgta
     2221 cttccgttat ccgcaataat gatttcataa tttgaataag acgttttttc gatgatcgaa
     2281 tccacacaag tttttagatc ttcatagcca ttcttcgttg gaatgatcac actgaccaaa
     2341 tcctcttgca agacgtcata agcaatctca taaaatccag aaattctgcc aggacgtacg
     2401 cttcctttga tgtttcttct agacagggca tctgtcaatg ctttcactcc ggaatcatag
     2461 atataacttt tggcttcgcc attacttgca gttgatcctg ggatcgtccg ccaatgatac
     2521 aagactcggt caatgtggta aatatgatct gcagggattt gttctgtcac acgaaggacc
     2581 aaatcataat cttgagaacc ttcatagcct ttacggaaac cgccaagttc tttaacaatg
     2641 cttgttcgat atacgcctaa gtgagagata taattatttc ccatcaatgt atcaggcgac
     2701 cagtctgctt tgaaatgcgg atcaaagcga ttgccatctg catcgatttt atcttcgtca
     2761 ctgtagatca catccagttc tggatgtacg tttagcactt ttgccacttc atataatgca
     2821 aatggcggta attcatcatc attatctaag agtgctataa attctccttc tgctatttcc
     2881 aatgcagaat tagttgccag actgatgtgt ccattttctt ttcggaaaac gactttgatt
     2941 ctatcatctt ttgcttgata ggattccaga cactttcgga tagctggatc agtagaagca
     3001 tcatcactga tacatagttc ccaatgatca tacgtttgat ccagtaccga atcaatgcat
     3061 ttttcgagcc atttgatttc aacgttatag acaggcataa gtatggaaat caatggacgg
     3121 tacgcaaatc cttgtgcttc tttcctttgc tctttcaaat ctggtttctc atgacgactg
     3181 atccactctg catagtcgat cgatcctggc ttcaattcca atttgacccg acggatcgct
     3241 ttttttacgc catgagtcct tgcataattt atcccttttt tcagtaatct tttttttctt
     3301 tcccatgaag attcaatgcc gtcatcataa gggtatttac cgtttaattt cacagctatg
     3361 ccattttgat gtctcgctgt ttggaagtct aagatggctt ttcctctcat tttccctgat
     3421 aaacgtacct taaaaccgct ttgggtctta acatcaagat tatataaatg attgatatcc
     3481 aatcttacga catgtgttgc ttcagcaatc acattttctt tttctacctt tatcgtaggg
     3541 atttctttgg tcacttcatc aattgcccag cccacaataa gcaaatcgcc agtggcccgt
     3601 tcacgagtga tttgatcgat aaatacggca atttcttttt tttgcacgat tcgtttactc
     3661 cttatcttta taagttaatc attatttaca tttcaatgat ccatgattgg ccattttgag
     3721 ctactgcttc cactaagaat gaacgttctt gctcgcaaag gattcgtaac tcaaatcccg
     3781 cttctgttcc ttttgcgagc tgttctgctt gattgacttc ttctcgtgaa acaggaattg
     3841 cttcgaaaaa tggcgattgg tcagctgata acttatagga caagggctgg cgatccgaat
     3901 tagcgattcc ccatccgatg atttttaata tttttgtttc tgcatcccag attttctcgt
     3961 caatacatat agaaagttct ttttcttgga tttttttcga agtttctttc ttacgtcgtc
     4021 ctagtctatg ttttattgtc cattcatcac tttccaacaa tgtgcaatag cgatttgtgg
     4081 ttgtttttaa cgtatggtct agtcttgtat tttcctcaag aagatgatga ttttctcttt
     4141 ctaattcgtc atttttctca gaaagctgct ttgtgacttc agccatggga cgaatcgttt
     4201 ccaacaattc tttcattgtt ggtgtcaatt ccccgataaa gcgatagtga caatggatag
     4261 tcacttcttt cccagaaata tccgttagaa cgaactctgg taattctttc ccatcaaaca
     4321 aataacaatc atttactgtt ttgactacag cattcgaatc gataaatgtt agtttttctc
     4381 cggcagcttc tgcgctgaat tcgataaagc taggttgttg cgcgaagcga aacaccattc
     4441 tttctgttgt ttctgatacg gggaaactta gtgtctcatt ttcccctgtg aaattattga
     4501 atggtatttg ttggattgtt tcatcttgtt tcgtttgctg ggttacttct aacactttta
     4561 cataattaga attttgtggc tctgcaatgg aactttgtgc tactggatgt ttcatcaatg
     4621 agaaaaagta ttggtagact tcaccaaatg gtcgcatttt gaaatcatag cgtacagctt
     4681 ccggcaattc ttcataagtt gattttaatt ctgtatcacc tacagctaag tacaaataat
     4741 cttcaatatt aatgaataat cccgcttttt caaaaacttt ttggaagcca tcatgagtat
     4801 aaaaagaatt atgggtttca tctaggagac cataagaagc ccacggcaat tggttattga
     4861 acaaatcgat catgacagaa ttatgcgcca agttagggaa tgtaatcagg atctctcctt
     4921 cttcattcaa aaattctctg actttcttca atgtttttcc aggatctacc aaatgttcta
     4981 aaacatccgc aaacaagacg taatcaaatg tctgtccagc ataatagttt gcccactcaa
     5041 aactttcgat atcaccataa aatccatctt gagcaaattc gctgacaaaa tcgaacaatt
     5101 ccttatctaa ctcaacaatg ctcacttcac attgttttgc tccgattaaa tgtttcgtta
     5161 atcggccgtt tccgggacca aactctaaaa ctttacttcc tggtttgatc tgtgcggcaa
     5221 tttttcccac actgctttgt tcatctaggt ccatttcaaa atcatatttc attgctatga
     5281 gtcccttctt cccatttatg gtctaagacc acgatgcctt ctccgatata agaaccgctg
     5341 acaaacaagt tcatgtactt tgtcttatat accaaaggaa ccgttgcatt ttcttcaaag
     5401 accgcaacgt caaaataata ttcaccagct aacaaaccta acttttcata ctccagataa
     5461 aagatattct ctcctttttt ccaaggaatc ttgataccat ctagtagagt attaagtccg
     5521 catacataaa aattgtcgac tgttcgtagc gcaattccta aaaccggatt tttgattgta
     5581 tcatctttta cttcataagt tactttaacg tatagttttt ctccttgttc cagcatatcc
     5641 accggatttt tgtctttatc caacaattcc gctttcgtca cgtcgacgat atctggagcg
     5701 gcgaaccgtt ctacttggtt tttattccga tccactgttt taactgattt tttctttaaa
     5761 aagttttcat aattcgtcgt tacttccatc gtatcgccat attcgacgac ttctccgttt
     5821 ttcaaccaat aggaacgatc acaaaaacga cgaatcgaac tgatatcatg agaaacgaat
     5881 aaaatcgtct ttcccgagtt cttgatttct gtaaattttt ccatacattt tagttgaaat
     5941 tcaagatcgc ctactgccaa tgcttcatcc acaatcaaaa tatcgggatt cacattgatt
     6001 gctacagcaa aagctagacg aacgaacatc ccgcttgaat aagtttttac aggttgataa
     6061 atatggtctc cgatgtcagc aaattcaatg atatcatcga ccatttcatc gacttcttct
     6121 cttgaaaatc ctagaaccat tccatttaaa tagatatttt catagccaga atactcagga
     6181 ttgaatcctg atcctagttc tagcaaagct gagatttttc cttcgatttc catcgtgccg
     6241 gatgttggtg tcagtactcc cgtaatgatt ttcaacattg tagattttcc cgatccgttt
     6301 tctcccacga aaccgatcat ctcacctttt tttatttcta cattcacgtt tttcaacgca
     6361 taaaatacat catgatagga tttcttaaaa ggattgaggg cttcacgaaa tcgatccgaa
     6421 ggttttgcgt acatattata agattttgtg atatctttta gtctaattgc atattctgtc
     6481 atagctattc tccttacagc acatctgaaa aatgtggttt caatctacgg aacactgcag
     6541 agccgattac gaacattact aacgtgaaaa cccagaaata caaagtatat gttccatgct
     6601 cccagaacca agcttcactt aaaaatgact cacgataacc ttgaacaaca tagtacagcg
     6661 gatttaattt caatatgctt acaagacctg aatcaaactg attagggctc cataatatcg
     6721 gtaatgtcca catgatcaac tgcagcacga tcgtaattaa ttgcatgata tcaggtaaaa
     6781 atggttggat cgatgccgtc agccaagtca atcctgtcaa gaaagcacac atgcaaaata
     6841 aataataaat cagctgtaaa gacattgctg aaggatatat accatttata agcgcgacag
     6901 ctatgccaat cagaatgaag aataagtggg tgtacagatt ggataggatt ttcgtcgtgg
     6961 gcaagatacg aatattgaat acgactttct ttactaaata agtatattcc cgaaaaacaa
     7021 ccgtagttga tgcaaatgca tctgagaaga agaaccaggg aatcatcccg gtaactaaat
     7081 atacgataaa tgggtatttt gcatcgatca tttgtgcttt cagtccaaca ccaaatacca
     7141 gccaataaat caatacagta aatagcggat tgataaacgc ccataagatt cctaaaaacg
     7201 atcctgcata tcttgatttg aagtcattga tcgaaaactg tagcaataat tttctgttct
     7261 gataaatgtc cttcataaat aaatacaaat ctttaaacat ttgtgttcct ccaagcgatt
     7321 ttcagtgaat tacagtcaag tgccatttta ccatattttt ggtaccgagc tgcgttctta
     7381 tttctttctt aattttctat gatacaagat tttctctaac gtttctattt tacagaagat
     7441 gctagacaat tgcaataaac caaaaaaggc tgcgacggtt gtcacagcct ctctcgtatt
     7501 ttgtgcctat caatgatagc gttggatcct ggaagaatga gcgctatctg gttgttcctc
     7561 ataagatgta tcatcataag tcatatcgtc atatgcctct tccataacat agaattcctc
     7621 cgcaacttct ggttcatcat atcgatgaac ggccatagcc aacaggatat aaataatcgg
     7681 aacccatggg tatgaagtca atgtatcctc cataaatgac tgcatcatca ccgcaaaaaa
     7741 ggcccagata aaagtcatct gcccgttgga taaatctgct ttcaacgtta agcgaccaat
     7801 aaaaaggata agcagcaaag cagcaatccc aaatcctatc aatccataga tattcaaaat
     7861 agaattataa ctattgtggt ttcctaaaaa ttggtttcct cgttggaaga taaatgtttt
     7921 cattcctagt aaaatttgtt cagatttttc tcctagcgtc tgatagaatt tatgccaaat
     7981 atcttctcga ccggtaaaca aattgacttt ttcagagaca gccgccagat aagaaatcgg
     8041 caaagctaaa agaatcgtaa ccgtgtaagc aataaatggc gcacgaatga cacgcatcaa
     8101 cgatttaggt aaaagatcca atatcgcaaa aacaatcaac gcgactaatg cacctctaga
     8161 ttggcagacc caaatcccga gaaaagctgc aacaactgct actgcaccga tgattcgtac
     8221 ataccatcgt tcaaaagaac tggcaaatcc actgatcaat aaagcaagag tcatcaaaga
     8281 acttccgatt atattcgtat tgatccaaat atcagaaagc gaattgcctg gagcccagat
     8341 gctttgtcct tctggtactt ggatctctgt aaatacacga tataacgtga ttccgaggaa
     8401 gaccgaaaag acaatcagat aaataccgcg atcccatttt gtccactcga tccatttaat
     8461 tactaaacaa aataaaataa tcaaaggaaa cagtaagtct ccaaataaag actcatttct
     8521 taattcccga aagtaagaaa agaaaatgaa aagggcaagc gcaacaccta gataaagaaa
     8581 atcgacgcct tttgctttat aaacatttaa aatgagcaaa atagccacac taaaagcgat
     8641 aagtaatgaa ctattgttat atataaacaa agttgctgga aaacctaacc ctgctgtcca
     8701 aacaaaaaag actagcgtca gtaacaaaag caagaaaccg acactcattt tgttgacaaa
     8761 tttttgtacg attgcttgaa ttatctccac tctcctttct actaacattt caggattatt
     8821 ctcagctagc ctattactgt acgactagct gattttcctg agtttgtgtt tgtggtacat
     8881 ctggataatc cggatattct actttaaaaa tcatataacc gtctttatcc ggtccatata
     8941 gctgcgtgaa ctgcgcttca tataattggt tgtaatcagt taatggtcgt ttactgatca
     9001 catagtcgat ccctaatttt tctgcgtctt caaaattcaa cgtgaccgaa aacatatctg
     9061 gacgatctaa aatgaacttt gtatcttcct tagcgataaa agctcttgta tgagcatacc
     9121 gattataata cttctcgtat tcttctttcg gatcgatttt cttccatgca gctatatctg
     9181 gataaaagcg tactgtattg aaggatttca ctcccaatgt tggggtgaag ttatataatt
     9241 catcttcaga caaccaaact ttaccaggat cttgttgatc gattttttgg atttcttttg
     9301 ccagtgtttt ttcaaaaatc gctcctgtac cttctacgac tggattgacg aaaaagccag
     9361 atacgataac taaggcggca aaagcgaggt aaaacaagcg tctccagcca aataatgcaa
     9421 aagctaaaat cactgttccc aacacagcaa ctgcgatggt ttcaaaatcc gaaaaataat
     9481 tatccatttt cgaatgcgta atcgcataga aataagacca tgcatttact gccaacaaaa
     9541 tgaatttata ccaaatagga ataacatctt ctctccaaat ataagcgata aaccagatcg
     9601 ataataaaca agccgcaaaa ctaaaagtaa ggatggctct taatggagga acataagaca
     9661 gcagagtgat ttctgctagc cattttggca acccaacggt aatccaaaag atagaaaaca
     9721 gacaaaaaat catcaaaata cgtcccagta acttttgttc gctatctttt ttgccaaata
     9781 acacaaatgg acttgctagg aatacagttg gaaaaaagtg atagaacagg gccacttcag
     9841 agttattcga atagttgata tcttcaaaag gaattttcca atttgtaagg aaagaaaaaa
     9901 attcgccaat cgtccacttt cctccagtac ttacccgttt tcctggatat aaggtattca
     9961 aagaagctaa caatgcgtct tttgacgtta gccagaaatg aaccatgact ccgccaataa
    10021 ataaaactgc ccctccaatc aacactacat ccaacagatc ccatgaaact ttcttgctat
    10081 agtaaataag caggccaaca aaataaaaaa tcagcaagta cgccagcatc acttgatggg
    10141 ctgggtaaat cactaaaata aacccaatac cgaaaataac caccaaaagc atcatcaacg
    10201 ttcgtagcca ttttttatca tgattgtaga aataatgata aaaagcagtc atcagtccca
    10261 aagtaaagaa aatcaaatcg cccacatgtt gcatgaacca ccactggact gctggagaaa
    10321 atgtcagcgc aaatgcaccg attagcgata gagctttttt tctcttcgtt aatatcatca
    10381 gtacttcgaa tccaagcaag accatgacga ctaatttcat cgcccaataa aaggaaagtc
    10441 ctcgatctct tccgaggaaa aagaatcccc agttgaatgg ttttccgata accgtaatat
    10501 cctttacagg agaattatac gcaatgatca tgttttgtcc gtttaatgaa taatcctcgt
    10561 tcaccaacgg aaagtcctct tcagcttgtg agaggtaaaa aggtgtctgc accagccatt
    10621 cgtctgaccg cacttcccga ttctgtccaa aaatcacatc agtttttttc ccatcgtctc
    10681 tttgcgacac atatttatcc caaacaccaa gtgaacttcc gtttaaatta aaagtaacga
    10741 tgaccacgat gactaaaacg ccaataagat agcggagttt tagaaacagg tcaagcacca
    10801 aattcgaatc tgcctggttc gcttttggct tttcttttcg tcgtgcactt cttgtttgtt
    10861 tttccatatc atgctccaaa ttctatttga taaaaggaaa ttacttattc atttttttgg
    10921 cagcttccag catttgcgca agtgcttctt gccaagttgg tatgacaaat cctaactctt
    10981 ctgtcttttt caggctcatg actgaatact gcggacgtgt tgctttttgc ggaaattctt
    11041 ctgacgtaac aggtgcaact tctacatctg tatctttcaa tatttctttt gcgaattcat
    11101 accaagaaca gctattttcg ttcgacaaat gataaatacc aaatggtgct ttttcttcga
    11161 tgacatatac cataaattct gccaaagtac gtgtccaagt tggtcgccca aactggtcat
    11221 ttactacagt cagtttgtcg cgggtttccg ctaggcgttg catcgtgaag acgaaattgt
    11281 gtccatagat cccgaaaacc caagaagttc gtatgatata atagtcttct aaaatctctt
    11341 ctactgcttt ttcgcctaat aatttcgtac gaccatattc atttagtgga ttcgtttggt
    11401 cgtctacttc gtattctaca tttttgtttt ttccatcgaa aacatagtct gtactgatat
    11461 agacgaattg agcattcaca gcttttgctg caagtgctac atttcttgta ccatcaacat
    11521 tgatcttctc atctaattct tttgcttctt cttctgcttt atctactgct gtgtaagctg
    11581 cacaatggaa aataacatca ggttttaatt ctgtgatgta agccattgtt ttctcggcat
    11641 ctgtgatatc caagtctttt gcatctgttg aaacatattg cagatttttt tcatctaata
    11701 aatggcgcaa ttctgtacct agttgcccat ttcctccagt taataaaaac atatgattat
    11761 ccgctccttg atttttattt taaaaaagag acaggcgagc gcctgcctca catgtgtttt
    11821 tccattaata cagtgacatt ttctctgtat tattgtccat ttttggcata tgctgcttca
    11881 acggcctctt tttctttttt ccaccagtct tcatgttctt cgtaccattt gatcgtgtca
    11941 gccaacccat cacggaagtt cgtaaactca ggcttccatc ctaactcttc ccgtaatttt
    12001 gtcgaatcga ttgcataacg caagtcgtgt cctgcacggt cgtttacatg gtcgtacgca
    12061 tcttttggtt gacccatcat ttctaagatc aattccatca cggtcttatt atcttcttca
    12121 ccgtctgcac cgatcagata ggtttcgcca attctgcctt tcgtcaaaat cagccacact
    12181 gctgaagaat gatcattcgt atggatccag tcacgaacat tctttccttc gccatataat
    12241 tttggacgaa taccgcttaa gatattcgtt acttggcgag gaataaattt ttcaatatgc
    12301 tgataaggac catagttgtt tgaacagttt gaaatggttg cacgtaagcc aaaagaacgc
    12361 acccatgctt ttactaacag atctgaacca gcttttgtag aagaatatgg acttgaagga
    12421 ttatatggtg tttccgcagt gaatttttct ccttcgcctt ccccatgtcc tggcaggtct
    12481 tcacgtaatg gaagatcgcc gtaaacctcg tcagtagata cgtgatgata gcgtacatca
    12541 tacttgcggc aagcttctaa aagtgtatac gtaccaatga tattcgtttg tacaaatggg
    12601 aaaggatctt ttaatgaatt atcgttatgg gattctgccg cgtaatgaac aactgcgtcc
    12661 gcttctttta ctagacggtc cactaattct gcatctgcaa catctcctac gaccaattct
    12721 acgcgatcgc taggaagtcc agccaagttc tctttgtttc ctgcataagt cagtttatcc
    12781 aaaacagtga catgcacttc tggatgattg tttaccacgt aatgaacgaa attagagccg
    12841 atgaatccgg cacctccggt aacaatgatg tttttcataa gaaaatttct ccttaattct
    12901 tagatttctc cgtaaacaaa tggattttct gcttcaaatt cttttaatgt cggatgacgg
    12961 tgatcttttt ccgacataat cgctcttgat ggatcaattg gccaatcaat cgctaaatct
    13021 ggatcatcaa atgcgatgcc cccatctgct tctgcattat agtaattatc acacttataa
    13081 aggaagttca catttggtgt caaagtaacg aatccatggg catatccttt aggtacaagc
    13141 aactgtcgat gattatgttc agataagata aatccgtccc attgaccata agtcgggcta
    13201 cctttgcgga tatccactac tacatctaaa actgctccag tgactacccg aatcaatttt
    13261 gtctgtgcag cttctccttt ttggaaatga agcccccgaa ggacaccagc ttcagctgat
    13321 agtgaatggt tgtcttgaac aaagtcatat ttcaatccat gttctgcaaa ttttgctttt
    13381 gaataacttt ctgtgaagaa accgcgatga tcgccaaaga cgtccatttc tacgattttg
    13441 acatctgtta attttgtatc tgttactttc atctgtaagg actccttatt cagcggccaa
    13501 attcaataaa tattgaccgt attgattttt tttcaatggc tgtgcaaggg ccagcaattg
    13561 ttccttatcg atgtagccca tacggtaagc aatttcttcc aaacaagcca cttttaagtt
    13621 ctgacgtttt tcaattgttt cgataaaggt agatgcttca agcaatgatt catgtgttcc
    13681 tgtatcaagc caagcaaatc cgcgtcccat aagttctacg gatagttttc ctttatccaa
    13741 atatgcttta ttgacatccg taatctctag ttctccacgt tctgaaggtt tgataccttt
    13801 agcgatagaa attacctcat tgtcataaaa ataaaggcca gttacagcgt aatttgattt
    13861 tggctgagca ggcttttctt caatcgacaa agcttgcata ttctcatcaa attctactac
    13921 accaaagcgt tctggatcat tgacatgata gccaaatact gtcgcgcctg tatcttttga
    13981 agctgctcgt tgcaacattt tagacagacc gccaccatag taaatattgt cccctaatac
    14041 caagcagaca ctatcatctc caataaattc ttctccaatg ataaacgctt gtgccaatcc
    14101 atctggactt tcttgtactg catattcgat ctgcagtcct aaatcatttc cgtttccaaa
    14161 taattccttg aaacgtggtg tatcttgtgg cgttgaaata atcagaattt ctttgattcc
    14221 cgccaacatc agagtagaca ttggataata aatcattggt ttgtcataaa tcggcatcaa
    14281 ttgtttcgat gttgcctttg ttaaagggta cagacgtgtt ccgctgcccc cagcaagaat
    14341 gattcccttc atggatatct gctctccttt ttgatttcaa atttcacttt gttattatct
    14401 catcaattgt tatatttgtc atggaaatat taaggattta aacatctttt atcaacgttt
    14461 aaaccatttt cgtttgatca acgccgtaat cagatgaaca cgccaagaaa ttttttgccg
    14521 taatttagaa tgtgtttcaa tctcactcgc gtttaaatca tgtcttcggt acagagttaa
    14581 cggttctctt aggaacccag tcttctttcc tcccagcaaa ccaatccaca tatcatgcat
    14641 cggcacttct gacgggatcg gcaaaatatc gggttttaaa gaagaccgga aagccatacc
    14701 agcaccaata tagtgacttc ttatcacatt attaagccat ccttctttgg aatttcgaaa
    14761 cgaaaaatac gaatcatgga tctcttgcag ctcgctgtcc acaatgatta aatcagaaac
    14821 tactactagc tgatccggat tctgctcaaa atagtcagtg atctttttca cttttgtctc
    14881 caaccaaaca tcatcttggt ctgccaaaaa gattagatct cctttcgtct gctggatcgc
    14941 ataaccaaaa ttagcgatca atccttgacg aggccccgaa aaaagctgga ttcgcttatc
    15001 tttttctgcg tattcagtta atatatccca tgtatcatct actgacccat catcagaaat
    15061 gactacttca tcttctgctg aaagctgaga taaaatacta tctaactgct ctcttaaata
    15121 tctgcctcca ttaaaggtag ctatacaaac cgaacgcacg aattcacacc ttccaattct
    15181 taacatatga gcgcaaagtc atccgccaga tatgccggtt tttgacggtc aagaactgtt
    15241 tagctgttct taacaataat tgtcgtcggt gactggataa atgcctttga tcatattcat
    15301 gataaaatcg attttctgct tgtaaaatag aacggtatcg attacttgaa actttttcat
    15361 agtctaaaac cgaaagatca tgttccattc gtacaggaag gatctccaca gttttttcca
    15421 actgatccac tttccaaaac agccaatgat ccaaaaaatc taaagaaaaa gcaggattaa
    15481 acccgccaat cttttttaga aatttgacag accaggcaac acctgaattg attgccatga
    15541 ttcgttcagc tgttgggcct tcaacttcta ctgcttgagc tgtacgattg atataacgat
    15601 ctgaatatag gggagaaact tgtcggctcc cagaaaaaat catcggaact gcagcaacac
    15661 attcatctgt aaaggttact ttacgcaata gttcaaaata atcgtcgact agcctcgtat
    15721 cctgatccag agtgacgaga caacgtgttc cttcatctgc ttgagacaac gcaaaattat
    15781 aagccaccgc taaaccagga ttttgcggat catgatgata aacgatattt tctttttcaa
    15841 acagctcatt ttgctgcctt tgaggactat tatcgtaaat aataagctga atttgctttt
    15901 cgctaagtgc tttgcttaat ggtccataag ctggagattg actgaatagt tgctgataaa
    15961 gcacaacgac tactaatatt tctggtttca tcagaaaaaa cgccaccccc acttattgaa
    16021 aaatttcatc atagaagtca aaaacacgcg aaacagtttt gcatttcgat gcgcaccctt
    16081 cccgtaaaaa tggataactg cataaaaggg tgtgtacaat aacttgtaac ctgctttttc
    16141 aaaactcaaa cacaagtcgt tgtcttcaaa gtacatgaag aaacgctcat caaacccctt
    16201 tatctcttta aatttatcaa tatcagctac cataaaacta cctgagatca tccgtacata
    16261 actattagca tcagtaggca atcctcggca ttcaaacaca gccagtcgtt tgtcaaactt
    16321 ttttttgacc catttgaaag gcacaaagcg caaaacgtaa tcaaaaacag ccagctgctc
    16381 acggatgagg tgctgagttg ttccatcttc attaagaatc ttaggtgcta acatcgcaca
    16441 ttccggatgt tctttcaata acaagaccaa tgctgtaact gtctcttcat ccactaaaat
    16501 atctgggtta caaataatgc catagcgaga atcgctagct atcagattgt gattatgtcc
    16561 ataaccaaat ccggaatttt catcatgaaa atagatatcg atgtagggat atttcctata
    16621 ctctgttagt ctttttttgt actcgggaag tgaattgttg tcaaaaattc ggatttttac
    16681 tgctgtttca tcttttacag ttgaaataaa attatctagt gtccgaaaaa ttttatcact
    16741 gtttgccgtc acaatactaa taaggatcgg ctggtttaat ttgttattca tctatatgac
    16801 ggttccattt cttcaaatat ttctgtttta cttgcgtacg gtactcttta ttccctaaaa
    16861 atcgcaatgt ttttaaaata aactgatgtt tctcattgat cagaccaatc aattcaaata
    16921 ataaaattgc agcaaacaac atggcaataa tcaatatgat cgttccgatt cttccagtat
    16981 aattcatgac catagctgtg aaagaaaaca tcaaagccat cgcataaatc gtcaggacag
    17041 cacctttatg agtaaagcct aaactgatta gtcggtgatg aagatgtttt ttatctgctg
    17101 tagaaaaaga aacacggttt gcttttctcc taatgatagc aaaaaacgtg tctgttaccg
    17161 gcactcccat gacgatcagc aaagaaatcg atgaaacaaa agtcacgttc ttcagccctt
    17221 gtagtgaaag gacggccatc ataaacccta ggaatagagc tcctgtatcg cctaaataaa
    17281 tctttgccgg ataaaaattg taaggaaaaa aaccgataat acttgctaca agacagaaaa
    17341 taacaactgg aatatacact gtagaagcat ggaggaaaaa atatccaatg attcctatcg
    17401 taattaaacc gatcaatgaa atccctgccg ccagcccatc caaaccgtca atcagattga
    17461 ccgcattcgt gataccgaag atccaaaaaa tcgttacggg aaaactaagc cagcctagtt
    17521 ggatatatcc aataaaagga agcgtgaagg aatcaatacg tatatctgct acaaaataaa
    17581 taataagaga agcaatcaag atccctatac ttttttgttt aggtgtcagc tcatatttat
    17641 catcgagcac tcctgtgact atgattacta aagaagcaat caagatcggt acaatatacg
    17701 attgagggat aattttacgg aataagaata aagtcgatat aacaaatgaa ataaagatag
    17761 gcagtccgcc cgctgaaggc attggaactt tatttattct tctttcattt ggtttgtcga
    17821 ccatacctgt ttgtactgaa atgattttga atacaggtgt taatatcagt gataataaaa
    17881 ttgtcagaaa aaatcttata accatactaa aagaaacaag catatgggca cctactttaa
    17941 ttaagagtca taattattat aaattatata tgtctttttg actatctttt tatatcttct
    18001 caaatgtccg caaagcctta ttttatcggc tctacgggca ttttgctttt gtggtaaacc
    18061 tcgcatatct aggtctatct tcttatattt tccctgtcaa tcgtggttaa aatcgtggta
    18121 aatacttcta tgcgattaga cgctgaacct ctgattttgc ggtatctatg gacgcatgag
    18181 cgtaccagtt cattgtgata ctgatgtttg aatgccccat gatatactgt aaatctttcg
    18241 ggttcatgtt cttgcttgcc agccttgtgc agaatgtatg gcgtagcgta tgcggtgtaa
    18301 tatgtggcaa gggattgtcc ttgtggtgct tgttgtattt ctttaccata cggataaata
    18361 aggcgttata atcaatggca acttttggat tacctttcgg attgataaac acaaaaccgc
    18421 tgtgtccgtc tacctctatc gactttatct ttggtcggct gttgataacc cgttgaaatg
    18481 cctgtatcgt ttctttgctt aatggcactt gccttatccc gcttttcgtc ttaggcgttt
    18541 caatataata gccctgttcc ttactcttta gtaactggtg gtcgataatc acaacttcat
    18601 tcttgaaatc aatgtcggct actgtcagtc cgcacagttc cgagatacga agtcctgtct
    18661 ttaacagtat cagcacatca tcataatgct tgtgatacac gttgtccgtc ttaataaatg
    18721 acaataaggc ttgctcctgt tcctctgtca atgcgacttt ctctttggta tcattttcta
    18781 tgacttcact tagcttgaaa tcaaaagggt ttttcctcac ataatcatct tgtatggcta
    18841 tgtagaatga cgcttttaac gagcgtttat ggttgttaat ggtattgtag gaaaagcctt
    18901 tgtctttcat gcgtaacgcc cattctttag cgtcagaggg ttttatcgta tcaatgctcc
    18961 tagcacctaa cttgtcctct tttaataacc gcatgagttg ttcccgttgt ttctgtgtgc
    19021 ttttcttcac atttgtcctc tgtgcgttct gtttggcata gagttggcaa agcgtcattt
    19081 tcttgcctgt gctgtcgata ccgtcctcaa tatcccgtct tatctgctgt tccagttctc
    19141 gaagtgaggg tttttcccgt tttccctttg gtgtcgggtc tgtgggtgtc aatctccaag
    19201 catacacata ttttgtgttt cccaatgcgt ctacatattt atataagtat tttccgtctg
    19261 ttcgttggct ctctccagta tgcaggatac gacctttgtt atcccgtctt ttttctttca
    19321 tggtgtctgc tcctttcctt gttggaaaga gccttgatat gacttgtgtt catcataaca
    19381 catacaaggc tcatttgcat tagattgcgt ccaatttatc aataaccttt tcaaactgtc
    19441 ggcgtttaat ctgtatgcgg ttgccattca taatgagcca gccagcgtcc ttgttttcct
    19501 ctgccaatcg tcttaacttg ttttctccaa tgcggaaata ctttgacgct tcttcaatgg
    19561 taagggtgta tttttcccat acgggaatat cctgtgtatt actcataaaa caccccctca
    19621 ctttcgatta cgtctgatat gtccgtacac atctgttcca caagcatttc ctgtttttca
    19681 gaaaggtggg tacattcatc aatcattttc atgtagtttg aacctgtcag cctgtcgatt
    19741 tcctctatca tcacgattga cggcatgacc tgtctttcca gccattgcag agtgcgttcc
    19801 atattgacag gtacagggtt catttcaaaa ggtaaactgt catgggaaat gaatatctcc
    19861 cacaatggat agtcaggaaa ttccacaaaa tcggtaatga gataaaacac cagcccatga
    19921 gggttatacg tcaataacag ttcttcaact gcctgtaccg cctttttgtc acgcagacga
    19981 atttcaaaac ggttgataat atcggtatgt ttctttttcg ttgcctgttc cttttgtttt
    20041 tcatacaggc agaaatattt tgtgcttgcc tttgagccga tataaagggt acttgctaaa
    20101 ttacggtttt ttccgcttaa ttttccacct tgtacatttt catagttttt acagcgacat
    20161 tccgcaccac catttctgta tttttcagat agcacaggaa tatccagcag accgcacata
    20221 tcattgattg ccaagtcaaa acgtcggata acaccgccac agtcaagaca gcggtttaag
    20281 aatgaatacc agtccctgtt ctgtgcctgt aagacatatt ccatgttgcg tgagcctgcc
    20341 ccttttaatt ctaaaaatac gcccatgtgt tcatcatcag aagccatgac cttgatttcc
    20401 ccgtaagcat aatgctcctt atagccataa tacccatagt cataatggat aaaataatct
    20461 gctttcatgc caagcacctt tcggataact tccaatgcgt cagtcgttgg aaaacggaca
    20521 gacagataat caacgagcag ataaaaaggc ttatcgcaga tatagtaatt cagacaacgc
    20581 aggataatgt tctgtgtttc cttgctggcg ttagcttttc cgttctcaaa gcggttgata
    20641 ctatatcttg atacatgggt aagacttgca agttctcctt gtgaaagacc gctggcaaga
    20701 cgtttgttct tcatttcaag tgcaaattct aaattgttca ttgtattcct ccaatcccac
    20761 aatagccttg aaatacctgt aaaccacctt tcaaaaagtg tgatatgtac tatcttctta
    20821 aaacctctca ttactactca caggtttggg ggtgtatggt ttttgacttg tcttttgtga
    20881 aatagtgccc ccctgttaga taacgggggc acagacaagg ctcgcaggct cgccagccga
    20941 caaagcattt cttccccttg acacaaggtg tcatggcggt aagcctgaga ggcttccgcc
    21001 accgtgtcag aaatactttg tcagcaagtt ctgttttgcc ttttctaaca agttccattt
    21061 caaccgatat tcatatctac ggatttaatc agataggtat ttctttttga atgtttttat
    21121 atttcattct attgtgctta ttcgattaca agaacatagt aattcttata tgcttatttg
    21181 tcaatatctt tttttgcctt tacaaatcat attaagcatg atagacttat aatagcgaag
    21241 atataacgag atatggaggg ttatgcaatg aaaataagcc atttaggaaa taatatacag
    21301 accataagaa aatttagggg aatgaaacaa caggaacttg cggacaaaat cggtatcaat
    21361 atgcagagcc tttccaagat tgaaagaggg ttgaactatc ctgcctatga aacgctggaa
    21421 aagataatgg aagtgctgga cgttacgccc aatgaattgt tgtcgggaga atggaagtat
    21481 gtcaatcaat ctgaaaagga agtctgtcag tttttaagag ccgaagaacg tctaaatgca
    21541 gaactgaaac atggacacta tgataacttc tttgacagcg aggaagaatg gctggaatat
    21601 gagttagaaa agttacggga atatattaca gactatatca acgggaaaag cattgaagcg
    21661 tccgaccttt acccaatcaa agaatttatc cagcatttga agtttcaaaa attattagac
    21721 cgctatgatg atttatacag tatggatatg tttggggaaa gcatagaggg acataagtat
    21781 aagacacctt atcaagtcgt gaaaatgata aacccgaact caaaagaaga tatggaactg
    21841 ttacgagaag tattaaggaa taacaatttt gatgatgagg acgagtaact tttttcctct
    21901 tgcgagaaga tacagcaaac aaaaagcccg atcaagagtg caaagcacca ccaatggtgg
    21961 caaagctctt gacagggctt tttcttttct gttgtgggca atcaagagga agaaagggat
    22021 aaaatctgat ttgtggtaaa tcaggcaaag gcaacgcagg tatcacagta tgaattgaca
    22081 ccgcataagg aaagcaactg gaagattgta gaataaatga aaaaggcttg cattctgcat
    22141 ttttataaaa ttgcaagtct ttatattgac aagcataaaa tactaagata tactttaatc
    22201 agaccaacgg tttgtattgg aggtaagtta tggcacgaaa taaatatcca gaagaaacag
    22261 taaaactcat tttagatgtt tctacaaaaa tgttttgtga aaaaggatat gataacacat
    22321 cattgcaaga tattatcaat gaaacaaaac tttctaaagg tgctatttat catcatttcg
    22381 attcaaagga agatatttta aaagcaattt ttcaaagaat tggaaatgaa aatgcagtaa
    22441 tatttgctaa aattagagat aacgatagtt taaacggttt agaaaagtta agagaaatat
    22501 ttaagacagc ggtattcaat tctaaccaaa gcgttttatt gacagtttct cctcaactat
    22561 taaacaatcc tcgttttctt gctatgcaaa tagaacaaat gtatgaaatt gtagtacctg
    22621 aatttgttga acccgtttta gaacaaggta tcaaggataa gtctttggag attgaaaatt
    22681 cacatgaaat tgcagaagtt atcatggtat taagtaatgt atggctcaac ccattagttg
    22741 aaatgacgga taaagagggt atggaaaaac gttgcaatac attcaatgcc cttttaaatg
    22801 gaataggcat agacacatta ttagacgaag aaacaatttc tgcatttata cagttttgta
    22861 aataactgta tttgttatcc ctatacgata gactgctcta taaaagagca gtttatttaa
    22921 accattatat aaaccgacgg tctgtttgat taaataaaaa ggagagattt tatgcaagca
    22981 gaaaaaaaac tttttaatcg tgattttata cttgttgtta taggacagat aatttcttta
    23041 tttggaaacg ctatcctacg atttgcatta ccgtactatt tgttaaaaga aactggttca
    23101 tcagctttgt ttggtattgc tacagcctgc tcttttttac ctatggttat tttgtcgctg
    23161 gtaggtggta ttattgctga caggacaaat aaaagaaaca ttatggtcat actagatttt
    23221 gtaacagcaa tcataatttt agtattttat ttgtcattgg gaaaaattcc tatcatacct
    23281 ttgtttattg ttgctttgat gttactttat ggtatttctg gtgcatatca accagcggta
    23341 caagcaagta ttcctctatt ggtatcatca gataaactta ccgtcggtaa tgctataata
    23401 aatcaagtaa atactttagc aaactttatc ggaccagcta taagcggagt agtttttcaa
    23461 ttttatggta tcaatcccat tttaattgtt agtattatat gttttacttt ttcagccatt
    23521 atggaaattt ttatccatat tccacaccaa aaaagacctc gtgaagtagg tgtaattgca
    23581 gttgctaaaa acgatttaaa agatagttat acctttttga aaaaggagaa acccattttt
    23641 atatccgtaa tctttttggt ttgcattttc aatttggttt taagtgctgt tttaattgtt
    23701 ggtctgccta tcttaatttc tgaagtttta aaaatgccag atacactata tggtttttca
    23761 caatcagcat tatcattagg tggtttatgt ggtggtattt taacagctct tgttgcttca
    23821 aagttaaaac taaaaaacct ttataccttg ttacttattt gttctgtttc tgttttactt
    23881 atgggaattt cgctattttt agaattgcct gcaatagttt cctactgggt agttacagtg
    23941 atgtgctttg tggctatggg tgtatctact atgtttgttg ttcaaattta cactcttgtt
    24001 cagacacaaa caccaccgca attagtagga aagataatgg ctacgctggt agcagttgct
    24061 atgtgcggtc aacctattgg acaaatggtt tacggtattt tattcgatgt atttatacaa
    24121 gaaacatggg ttgttatgtt tgtagcttct attgcttcaa tcatgatttc aatatattca
    24181 aaaagaattt ttcaaaagtt agagatataa tacaaggggt taaatagtat gttaggctaa
    24241 aaacaatata taaa
//
LOCUS       DAZUKA010000025        23697 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000025 DAZUKA010000000
VERSION     DAZUKA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.025"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..264)
                     /locus_tag="ACG8PN_000831"
     CDS             complement(<1..264)
                     /locus_tag="ACG8PN_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861007.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFL"
     gene            complement(439..2574)
                     /locus_tag="ACG8PN_000832"
     CDS             complement(439..2574)
                     /locus_tag="ACG8PN_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4861008.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2657..4312)
                     /locus_tag="ACG8PN_000833"
     CDS             complement(2657..4312)
                     /locus_tag="ACG8PN_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4861009.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4314..4628)
                     /locus_tag="ACG8PN_000834"
     CDS             complement(4314..4628)
                     /locus_tag="ACG8PN_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4861010.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4798..5058
                     /locus_tag="ACG8PN_000835"
     CDS             4798..5058
                     /locus_tag="ACG8PN_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4861011.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5072..5386
                     /locus_tag="ACG8PN_000836"
     CDS             5072..5386
                     /locus_tag="ACG8PN_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4861012.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6116..6670)
                     /locus_tag="ACG8PN_000837"
     CDS             complement(6116..6670)
                     /locus_tag="ACG8PN_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4861013.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6796..8725)
                     /locus_tag="ACG8PN_000838"
                     /pseudo
     CDS             complement(6796..8725)
                     /locus_tag="ACG8PN_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(9031..9747)
                     /locus_tag="ACG8PN_000839"
     CDS             complement(9031..9747)
                     /locus_tag="ACG8PN_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4861014.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9748..11571)
                     /gene="glpO"
                     /locus_tag="ACG8PN_000840"
     CDS             complement(9748..11571)
                     /gene="glpO"
                     /locus_tag="ACG8PN_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4861015.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11585..13081)
                     /gene="glpK"
                     /locus_tag="ACG8PN_000841"
     CDS             complement(11585..13081)
                     /gene="glpK"
                     /locus_tag="ACG8PN_000841"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4861016.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13217..14677)
                     /locus_tag="ACG8PN_000842"
     CDS             complement(13217..14677)
                     /locus_tag="ACG8PN_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861017.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14667..15962)
                     /locus_tag="ACG8PN_000843"
     CDS             complement(14667..15962)
                     /locus_tag="ACG8PN_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4861018.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16294..16971)
                     /locus_tag="ACG8PN_000844"
     CDS             complement(16294..16971)
                     /locus_tag="ACG8PN_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4861019.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(17001..18092)
                     /locus_tag="ACG8PN_000845"
     CDS             complement(17001..18092)
                     /locus_tag="ACG8PN_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4861020.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(18193..18627)
                     /locus_tag="ACG8PN_000846"
     CDS             complement(18193..18627)
                     /locus_tag="ACG8PN_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4861021.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19211..20668)
                     /locus_tag="ACG8PN_000847"
     CDS             complement(19211..20668)
                     /locus_tag="ACG8PN_000847"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4861022.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20665..22560)
                     /locus_tag="ACG8PN_000848"
     CDS             complement(20665..22560)
                     /locus_tag="ACG8PN_000848"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4861023.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22618..>23697)
                     /locus_tag="ACG8PN_000849"
     CDS             complement(22618..>23697)
                     /locus_tag="ACG8PN_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4861024.1"
                     /translation="LRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT         6689 a         5105 c         3987 g         7916 t
ORIGIN      
        1 caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca ttttcttttc
       61 tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta atgggaaaaa
      121 gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta acgtttgcca
      181 aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa ttgatgcaac
      241 ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac gacgtaggag
      301 tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc taagaaaaag
      361 aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca gttgttcttt
      421 ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt tcattcctct
      481 agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt tttcaaatgg
      541 atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa tgatttggca
      601 gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg tttcctcttc
      661 tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct cagctagtat
      721 tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc cttgcggtcg
      781 gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa gctctgtgat
      841 tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca gatggtagtc
      901 gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg cctgattctc
      961 gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac ctaattgtgc
     1021 cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta catttacctt
     1081 ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag tacgtgtggg
     1141 ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca actgatgctc
     1201 atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt tttgtaaaag
     1261 tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt taccgaataa
     1321 ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag gactttttgc
     1381 ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg cttgaagcct
     1441 atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg tttttttaga
     1501 aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat ctaaaaacaa
     1561 aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt ctgtctgctc
     1621 cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta tttcctctgc
     1681 tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt cacctaatga
     1741 tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa tcagcaagtc
     1801 atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct gcatgatagc
     1861 agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt tcacatcccg
     1921 aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt cattggtctc
     1981 atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg tatcaaaaaa
     2041 cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa ccgtatcgat
     2101 tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa aaagttcggc
     2161 aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga aatttgctgt
     2221 accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga cttttccaaa
     2281 gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt ttttctgttt
     2341 gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag tatccaggtt
     2401 atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca acgactggtt
     2461 tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat caattagctt
     2521 ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga ccatacacat
     2581 tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga gtcgaatgca
     2641 agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg acagtttgat
     2701 ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc aagattcttt
     2761 ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt tggctgtcta
     2821 attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc ttgaatttgc
     2881 ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt gctagttctg
     2941 tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc attgctgcat
     3001 atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac gtcaattcta
     3061 attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa atatcgatcc
     3121 gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca gtctctggat
     3181 ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca tgatcttttc
     3241 ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt tcacttaaac
     3301 cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata ccgccttgat
     3361 ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga ttggctggtg
     3421 aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg atagcatccc
     3481 ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg atacccccgc
     3541 gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga acaccaatgg
     3601 ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat cgtttcccgt
     3661 ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga tgagacaatt
     3721 ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt ggtgcttgtt
     3781 cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc aatccgatga
     3841 atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc atcactttat
     3901 cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc cctggaatcg
     3961 atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt tcaatgtgtt
     4021 tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct gtcacttcat
     4081 gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt tggacaagaa
     4141 gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa tttgcaaaag
     4201 aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat cggcgaagcc
     4261 gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc atcttatttc
     4321 cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac tacatcataa
     4381 ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg acaaatcaca
     4441 tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg aacattttta
     4501 gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag taacgctgga
     4561 ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga ttgaaatagc
     4621 aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt atattaaaca
     4681 ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac tttttatatt
     4741 tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga atgtgttatg
     4801 gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca aattcgtggt
     4861 atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca attaagcgct
     4921 gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct gaaaaattgc
     4981 tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca agcgatcaag
     5041 atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata ttgatgaatt
     5101 agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt ctccagaaac
     5161 gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact ctgccaatgc
     5221 agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta tgcgtgctaa
     5281 tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag gtgtccttga
     5341 tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag tagaggttgc
     5401 gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct acttctggaa
     5461 aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga acagccaaac
     5521 aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
     5581 aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt agagaaaaaa
     5641 acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag gtcagctgat
     5701 tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta ttaagaaaaa
     5761 ttttgtgaaa atggatttct acatttttgc ataaatttgg cttaataccg agaaactggc
     5821 ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
     5881 aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac cgaaggtcag
     5941 ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg aatcacaaga
     6001 aataatttca aaaacaactt ctcctgtttt tggttcggtt ggcttattat cgagaagtag
     6061 ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc cttttttatt
     6121 ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt aaaagaccgt
     6181 aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg aaaagcagca
     6241 aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac ttcgctccac
     6301 ttaatttgat atttttagat agcccgatta atccagctac aaacataaag ccttctttta
     6361 tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata gacaaaatca
     6421 gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag tctgttacct
     6481 gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag acaacaatga
     6541 ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc ggcagcaaaa
     6601 aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc cagtctgtgc
     6661 ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt tagcttacac
     6721 ggggaataaa accaatgatt tcactgtttt tatttttctt tacagacact gatattatga
     6781 gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt cctgtcgttt
     6841 gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta ggtcacgctg
     6901 ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg tcgggtacag
     6961 atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta tttttttgtc
     7021 taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt ctttttggta
     7081 ttcattgaca aatatccgat caccttttgc aaagagctga cgttcttttt tattctcctt
     7141 cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat aggctttttc
     7201 cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt tatgagcaat
     7261 ccacaaagct tggctttctc ctgtttcttc caacccgcaa ctgatattta ggacgaagtg
     7321 cttcccgatc gaaagccata gcagccggca caaaatcttc atgatctccg gcgaatttct
     7381 tgatttctcc ataatgggtc gtggcaatga tcaatgcccc ttgctcatac atagattcca
     7441 tgattgcgat cgctagtgca gccccttcat ttggctctgt tccactgcca atctcatcta
     7501 aaagtaccaa cgtattcctt tttatctttt tcaaaatagc agcgatgttt tgcatatgac
     7561 cagaaaatgt actcaacgca ttttctaaat tttggtggtc accgacatcg acgaaaatct
     7621 catcaaagac agcaagctct gtcccttctt ttgccggaac ttgcataccg aacatcgcca
     7681 tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt gcctccggca tttgctccgg
     7741 tgataatcaa gcctcgatag tctttaccga tttcaacatc caatggtacc gcatgctcag
     7801 gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc cgatttattg attctaggtg
     7861 tgcttccatt gatttctcgg ctaaattttg cccgagcaaa gatgatatct aacaccgtga
     7921 tcgtttcgat caataaatcg atcacttctt cgttttctgc gatagcacca gtcaatgccg
     7981 ccaggacttg atattcttca ctgatttctt cagcttttaa caactgatag tgttcattca
     8041 atttagaaat cacagttggt tcaataaaaa ccgtcgttct tttatttgat tcatcgataa
     8101 tcgttccatc tattttattt ttatagctgg ctttgatagg aatcgtatag tattcgcctt
     8161 tttgaacaat cattgcttct tggatcattt ctttgttttt cggatgacgc aagaatttca
     8221 gtaattttga ctggatttct ttttcgatta gttgtaattg tttgcgtact ttacgcaaat
     8281 tacgtgacgc gtcgtcactg actttattat tttttatttt ctggtcaatc aactcttcta
     8341 catttatcaa atcaggaaga tgtttgctat aagcaaacaa caagggtgtc tggtattgat
     8401 tcttatcaaa aaactttgtg atcatccgac tgcttctaag aaaatccgca tattcgatca
     8461 aatcggttgg ctgcagaatc aacccttttt tgacttgttc tgtcagtgca tcgatcctcg
     8521 gcaaccccat aaatggaaca tgctgactgc tttctaagat caagcgtgct tctcttgttt
     8581 cttcttgacg atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt
     8641 ttttgctata gtttccaatt gctcttgctt ggatttcctt tttgatccga tcaaattgta
     8701 attgactaat catttcgttg tccatattct ctccttttct tgaagagata cctgtcccaa
     8761 aataaataag ggcctagaga taaataaaag gatagaacac agcttaaaaa agtcatgtcc
     8821 tatccttatt agaatacaaa gatattgttt tcctgacagg taccgtttca taagcacacc
     8881 aaataaactt tgtttattgg ctattcgact tactgattag ataccagcta cactcacaaa
     8941 acatacccca ttcttctagg tcttatttat tttccactta actatagcaa gacacatttt
     9001 ggttgtcaaa ctttagtatg ttttatagat ttacaccatt aatgaataga cacctaccgc
     9061 gataattgct ccgatcattg gacctgcgat tggtacccat gaatatgccc aatctgaagt
     9121 tcctttatta gctattggca ataccgcatg tgctaatcga ggaccaaagt cccgagcagg
     9181 gttgattgca tatcctgttg gccctcctaa tgaaagaccg atggctaaga tcaatccacc
     9241 tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag
     9301 aactaataca aatgtaccaa ttaattctgt gatcacattt gctggataat tccgtatagc
     9361 aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg
     9421 caaataagaa agccaaacaa gaatggcccc tgcaaaagct cctaaaactt gcgcaatgat
     9481 gaaaggaacg acaagattcc aactaattgc ccctgtcatc gccatagcta gagagactgc
     9541 aggattcaaa tgagctgggc ccatgaatcc agaaatatag acagccattg ttacagcgag
     9601 tccccaacca aatgcgatga caatccagcc agaagcctgt gctttacttt tgtttaaatt
     9661 cacagccgca cacacaccat ctccaagcaa aactaggatc agtgtcccaa taaactcccc
     9721 catgatttga gtcatatcac tgttcattta tttctccccc tttagttccc gcagatctga
     9781 ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc
     9841 aaaatatcca gcaattgcat cgatcacagg ttgcttgatc tcatcaagct gatctcgttc
     9901 aaacaacaga tgatttgtac gacgaatgag ataatcgcct ggggccaaaa ccatttcttc
     9961 ttctaaacca taacgtaacc gagctgactc agcgagactc aaaccaggat aaggtgccat
    10021 ttcttttgct aattcaaaga tacgtcgggc attagtcccg taaaaatcag cgatataagt
    10081 agcatcttct tcctctaaac cggctgctac tccttctttt gctaattctg tcactgtttc
    10141 ttctaattta gttggatcga agtttccacc tgaaatctga tattttttag agtcgatttc
    10201 tttcgtctct attccgtatt cttcttttaa taattggcgt atcaatcgca aagctccttc
    10261 tgccatctta cggtaatcgg tgattttccc accagataaa gtcagcaaac catctggttc
    10321 tctttctaaa gaactacctc tggaaatcgt agaaggtgct ttttcatcac ggctgttttc
    10381 caaatggttc aacacgtctt ctacttcagc acgagaaact ttgttttcct tatattcact
    10441 tactgtatcg acaactttat tgaagctctt gtctgaaatc gatccattat ctccaccatt
    10501 gtaatcagaa ccagaattac caatcaacag tggacgaagt cctgcccagc ttgcttcgat
    10561 atcattcaat gtgatatttg cttctggata gcgatgattg atcacatcca atagataatc
    10621 tacatcttct tgtgtgactt tcggatccgt aaaatctcct tggtaatccg tatctgtcgt
    10681 accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc atccgcccat cttgctttcc
    10741 tgtatcgaag tatgtgggtt gcggtacagg cagttttttc gcatctacaa ctaaatggat
    10801 ccctttagtt ggacgcattt taggagagac tggacgcgta aaattcaagt ttcgtacttt
    10861 atctacccaa ggaccactcg tattgatcac taattttgct ttgatttcga tcacttcatc
    10921 tgtcagcagg tcacgagctt tgacgccaac gatttgatcg ccatcatata aaaagccaac
    10981 cgctttcatt ttacttacta gataagcccc atcttcggca gcctttttga tattatcaat
    11041 cactaaacgg gcatcattgt tgcggaaatc tagatagaca cccgcgcctt ttagcccttc
    11101 ttttttcaaa aacggttctc tttccaatac ttcttctggt gtcagcgtat aattctcata
    11161 tttggttcct gtcacatttg ccagtttgtc ataaaggtcc attgctactt tgaccgagaa
    11221 catattgaag gttgttgctc cttcgccctc atagattggc aaaagcattg gatctggttt
    11281 tgggatgtgt ggggcaatac tttgtacgac tgcacgttca ccaactgtgt cagctactac
    11341 ttccacatca aatgtcttca gataacgaat accgccatga acaagtttgg tcgagcgaga
    11401 ggaagtccct tctgcaaaat cctgcatttc gatcaatcct gttttgattc ctgatgctgc
    11461 tgactggact gccacaccgg caccagtgat tccgccaccg atgatcaaca gatccaattc
    11521 ttcttgctgc attttttgaa tgctatcttg tcttgtcttg tttgaaaaca tatggatttc
    11581 ctccttattc aaatgcttgg gtagctttta ctgctttttt ccaaccgttg taaagttttt
    11641 ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc agcttcataa aattcgcgga
    11701 tctcgtctgt atctttccaa taaccgacag ctaatcctgc taaaaatgct gcccctagcg
    11761 ccgttgtttc caagttttct gctcgttgga tcggtacatt caagatatct gtttggaatt
    11821 gcatcagata ttcattgttt gctgcaccgc catctacttt caatacagga atcttgattc
    11881 cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg ataagcaatc gactgtaaag
    11941 tagctttaat gatatcttct tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg
    12001 ctgcctgatc ccaataagga gcacctagcc caacgaatgc aggaacaaca taaacctcat
    12061 cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct agctttttgc aacatttgaa
    12121 gtccatctct cagccattga atcgaagaac cagccacaaa aatacttcct tctaaagcat
    12181 agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt
    12241 gcggtttttc accggtattc atgacaataa atgaacctgt accataagta ttcttgacca
    12301 tgcctggttc gaatgccatt tgtccgaata atgcggcttg ctggtctcct gccatccctg
    12361 aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt ataaccataa acttctgagt
    12421 tagaagtgac ttttggtaac atcactcgag gaatatttag taaatctaaa atctcctgat
    12481 cccaatctag ctgatggata ttgaacaaca tcgtccgact ggcattcgag taatcagtaa
    12541 catgtgcttg tccatcggtc agtttccata ctaaccaaga atcgatcgtt ccaaacatca
    12601 attcgccttt ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc cagcgtatct
    12661 tcgttgctga gaaataggca tcgatcacta accctgtttt ttcgtggaaa aattcttgat
    12721 agccttcatt ttttaagtta tctgcaattc cagctgactg tctggattgc caaacaactg
    12781 catgatagat tggacgtcct gtatcttttt cccatattac tgttgtttca cgctggttcg
    12841 ttatgccaat cccagcaatc tgatttggtt tgatccctga ttcgatgaac gctccggcaa
    12901 taactgactg tacagaattc cagatttcat ttgcatcatg ttccacccaa ctttcttgcg
    12961 ggaaatattg cgtgaactct ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga
    13021 taattgctcg tgagctagtt gtaccttggt caatcgccat gatatacgtt gattctgtca
    13081 ttgtgattcc ctccattata tgcaaacgct ttctttaact actcttatca tactactgtt
    13141 ttaaaaaaag aaactgtcaa aattttattc tttttttctt tttttgacag ttttttatta
    13201 tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt ctctattctt cttttcaatc
    13261 gaataatgtc gtaaggtgac gcaaattctg acagataata ttcacactgt tttttgtttc
    13321 tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc ttcataactt ccaatctcaa
    13381 taccgccgat tcccctcagt tctctgatca gatattgttg aaaagcaata cgataaatcg
    13441 gcagactttc taaatcgatt ccaatcacta ctggttttgc aatataaaaa tcgatcatca
    13501 aaagaacatt ggcatactgg atcatcaggt aactaagctc actatcttca gctcttttcc
    13561 tcggcaattg ctgcaccgct gtttcctgta atttcgccag taactgtgtt aaggaatgcc
    13621 cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc atagacttcg atctttccaa
    13681 caaagaaaaa ccattcattg ttgatctggg aaatttggta gcctactgcc ttttcttctt
    13741 tgattttcaa acgacgagga cgataatgcc ataacatcgt ttcccgaatg taagtatcta
    13801 aaacagcggt tggtaacttt ttgctccgtg tcaaatcata ctgataaaat atttcttcgt
    13861 ctactatcga aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat acattcagtt
    13921 ctgctgctgt tcgactaaga tgcatcacga tgattggatc gattgcttga taaagccggt
    13981 ccgactgata gagtaatttc ttttctttca aagttgcgta cgtacttttt tcattcaata
    14041 gtcttttttt cgtaatcccg agccagcaag caatctgtgc ttctgctgat gccgtaaacg
    14101 ttgtttctaa tacacggtcc aatcctagaa tgaacggtct tttttctggt gtattttgca
    14161 aaaaaagcgg tcgctgatct tccgggatat attggaatag ttcataataa aaatatcgta
    14221 tctgtagctc ttctccgtat aattgaccgt ttttgatttg taatccaaat tcctctaaga
    14281 gctggttcaa ttctttgatc tttcgaaaaa cagaagactc actgatatta aacgctgtcg
    14341 ctaatcgaac gatggatact tgctgatgtt ctaataaata gacgagcatc ttaaacttca
    14401 agctttcctg caataaaaac aatagaattt tttccaaact tacatcttcg tccaacacaa
    14461 gcagcgcctg atactcattt cgctgtactt ctactttttt ttccatcatt cggccaatca
    14521 attcaatatc ttccaaatat tgatcaaacg aggcttttga cagttggaca tgctctctca
    14581 aaccattgat agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc aggatctgta
    14641 actgctcttt tctttctaat aatgcttcaa tcttcatagg cagtattcgc cacctttgac
    14701 cacaccatat ctggcacacg gtattctggc tggaaaaata gctcatgttc ttctaactgt
    14761 tcaaacgtcc atccttcttc tactgcaatt gtcagttggt caagaaggtg gaacatctct
    14821 tttttgcttt ggagctgtgc tccaagaatc tttttttcag aatcaaatag aaatttgagt
    14881 ttaactttgc tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt
    14941 tggggataaa aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa atagccaaac
    15001 cactgattgc cgactggacg ataggttccc tgatcctttg tctgtgagcc actaattgtc
    15061 ttgctgacat ttcgtgctgt acggacagcg ttatttacta gagaaacata cattttttca
    15121 tttgttggac gaaaagttac ttggatcgca tcgccaatcg cataaacgtc tttttgagac
    15181 gtttgcaagt agtcatctac ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc
    15241 cacatctgat tgtcgggacg agaatgatta gccagcaaaa caagatcata gttctccttt
    15301 cttttttccg tctctacaat agcggtttca ttttcggtca aagacacaac ttgttcttct
    15361 aaaaaaagtg aaagcccttg atttttcatt tcctttacta acggttctgt catttcagga
    15421 tctagataac gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg tttttttctt
    15481 gatgccaacc cttcagctat ttcgattcct gcttgtcctg caccaataat tgcaattttc
    15541 tttgcattca tgatcttttg ctgaagagcc gttacgtttt gtatcttttt aactgggtga
    15601 ataagcgttg attctgcaga aatatttcga aacttttgat ttgaaccagt cgctagaatc
    15661 aaacgatcaa aatccaattt ttttcctcct gctaaaacga cttttttttc tttcaatcct
    15721 attactgtct tttctgtgta aacatggatt cgatagcggt cagtcagttc ttctttcgtg
    15781 atccaagaaa gttgtttttc actggtaacc tttcctttta atatcaaatt gaacccgctg
    15841 ggaatccatc ctaacttcga ttgcttttca atcaaatata tttcacttgt tggcatcaat
    15901 tggcgaagat aaatagccgc atgaatccct gcaaacgacc cgccgacaat tacaatgttc
    15961 atctgcattc ctctttctta taaggtgttc tactgctaac ttctcaatca gaaaacacag
    16021 tattcataac tcgctttcaa ccttatgata catagcgatg cagcagaatc aaccctctcc
    16081 atcataccgt gttctaggtc tgcttctcaa taatgtacta gtggacctaa ttgaagagta
    16141 tcatttctga agagatgttt caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt
    16201 acgtacctct gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta
    16261 atgtaatagt tagaatagac acatcattta tttttaattc tttaatgctt caacaactgc
    16321 ttcgcctaat tgatgagcag attgaggatt ttgccctgta agaagccgcc catcaatttg
    16381 aaccacttct gaaaaagctg ctccagactg atagtttgct cctttttctt tcaaagcatc
    16441 ttctgctaag aatggtactt cattcgttgt accatttgct tcctcttctt cattactgaa
    16501 cccagtcact gtacgtccag ctaaaataga ttttccagac tcatctgtaa ctggcaacaa
    16561 tcctacaaca ccgtgacaaa cagctgagac cagcccttca tttcgataga tttgtgaagc
    16621 gactttccca agatctattg cttctggaaa atcccacatt gttccgtggc caccagcata
    16681 ataaaccgct gcataatcat ttgcatccac atcatcagga cgtaaggtat ttcccaaggc
    16741 ccgatttctg aaatcttcat cttcataata ggcccaatcg atttcatcca tattcaaaac
    16801 actttgcggt tctaaaggta catatccgcc tttaggactg acaaaatctg catcgatccc
    16861 attttttact aatacatcat aaaaatgagt cagttcactt aaccacaaac ctgtaggacg
    16921 ctcgatcttt tcgtactttt ctacatttgt taatacgatc aatactttca ttttcccatc
    16981 ctctttctat aaaaattgcg ttattgataa cgtgctttca agaaattata taatgggctt
    17041 ggcaaataaa gctctttata tgctttttca aaaggtttcg ttacaaattc atcctcacgg
    17101 ttttccaata atttctctgc ccagtcagga tcgacgatca tttgctgtcc aattgctgca
    17161 agttctgcat atttcaatac ctgctctaca tcttcgcgat tgcggatccc accgacacca
    17221 actaaaggga tctttccttg aatcgtgtta tggaccactt caagaatcga tttatcgttg
    17281 tatttatcag aacgagctat tcgatcatac atatttaatg aaacatgcag ataatctagt
    17341 tttgactcac gtaatcgttc aagaagccag atcgtgtctt caaaacggat acctggtgtt
    17401 tcatattctt ctggtgagaa acgataccct acgatgaatg gttttttagc atattgttca
    17461 accgctgaga aaaccgttgt taataattct tcaataaaac gataacgatt ttccaagcta
    17521 ccgccccatt gatcctctcg tctattcgaa tgcggagaga aaaattgttg gatcaaatac
    17581 gtatttgctc catgcaactc gattccatca aatcctgatt caatcgctct acgtgtggct
    17641 ttgccaaatg cttggattgt ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc
    17701 gcatcaggac gttttgctgc aactgcactt gcagatacag gctgttctcc agataaagtt
    17761 gctcgacttg tcattcgtcc accgtggaaa atctgaataa tcgcttttgc gccttctcct
    17821 tggattgctt tcgctagctc acttaatcga ggaatcattt catcatgagc gatgctgagt
    17881 tcacctggcc agccttttcc gatatcttgg acatttgcag caccagtaat aacagcacca
    17941 agacctttag aacgttgttt ataataagtg atctcatctt cggtgatcac tccattgaaa
    18001 aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg aactcctgaa
    18061 ggtaaagtaa ttgattgatc aaattgtgac attgtcattc ctcccataat tcgctctgat
    18121 taacctatcg agaatcatct atcattaaac cactcgatta gttgtacatg caactaattt
    18181 gcttaaaaaa ttttattttt tttgtaactt atctgttgca tcattcattg tctgggctaa
    18241 ttctttactt gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt cttcccagct
    18301 accatcgact tccttagcaa attggcgtcc ttgttctgta agataaacta atgacaagcg
    18361 accttcgcta actgttttga cgtatccttt atattctaat ttttcaacaa aacgagcaat
    18421 cgttgatggg gcggaataga gcatttgtgc aatatctttt tgtggtattc catctttttc
    18481 ttttaatatc aataaaataa aaccataagt tggtgccaga cctgtccttc taaacgcttc
    18541 atcagctaat ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa gacattcatc
    18601 taacgctttt tcaattggct tttccatttc atcccctcca ttggcgtaca tatcatagta
    18661 tgatttttat tttttgtcaa aaaacacgga ataatgtccg cctcctctgt ctagatttga
    18721 aaaaaagaag tagccatctt caaagaattt cggcttgttt tcgacaaaac tccttctgga
    18781 acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg
    18841 tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgaaaaa ctggcttttg
    18901 aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc
    18961 aaacaaaaat agttcctcat attttgtcgt tttttgagct aatgccgatg gtcagctgat
    19021 tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa
    19081 tttcaaaaaa cagcttctca tgttttatga ctatggtgac ttgtgatcga ggagttggca
    19141 ggagaatact gtttattcgt aaagaagggg ctgtgacaag acttttgtca cagtccctta
    19201 tttcctttta ttataggatt tcgccatttg attggatgac ttgtctgtac caatcaaatg
    19261 attttttctt taaccgtttc tttgtgccat tcccttcgtc atcttgatca acatagatat
    19321 atccataacg ttttgacatt tgtgaagtag aagcgctgac gatatcaatg cagccccacg
    19381 acgtataacc aaataaatct actccatctt tgatagcttc cttcatctgc tcaatatgct
    19441 gcttcaaata gtcaatgcgg taatcgtctt gaacggttcc atcttcttct actttgtcta
    19501 tagctccgag tccgttttca gctatgaaca gtggtttttg atacctatca tataattgat
    19561 tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc ccattcactt gatggaagat
    19621 acggattttt aattcctcca gttattaagt ttccttggac cccttcccaa ttttctggat
    19681 gagcactgac tgcactagac atgtagtagc taaaagaaac aaaatcaaca gggtaggctg
    19741 ctaagatttc ttcatcacct acttctattt ttaagttgac acctttttct tcaaacattc
    19801 gttgcgtata cgctggataa tatcctcttg cttgaacatc actaaagaac aaagaagttc
    19861 tttgctgctc aattgttttt tgaatatcat ccggatcaca agaatacgga acaagaagct
    19921 gatagctgat catacaaccg acttgtgaac ctttgataat ttcatgacaa gcttttgtag
    19981 ctaaagcact tgctataaac tgatggtgtg caccttggta tttcgcttgt tccaaatggg
    20041 gatggttttc ttcaattact cctacgctaa caaaagggtg atgttttaca cagttgatct
    20101 cattgaaagt catccaatat tttactttat ttttatagcg tttgaatact gtttcagcga
    20161 aacgaacgta aaactgaata agctttcgat taggccatcc accatattct gtaaccaaat
    20221 ataacggcat ttcataatgg gaaagagaaa cgactggctc aatcccatat tttagacatt
    20281 cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt gggtgtttct tcatcaccat
    20341 gaggaaaaat tcttgtccat gcgatagaca tccgatagca tttaaaaccc atttcagcaa
    20401 ataaacggat gtcttcttta aagtgatgat agaaatcatt tccgtgtctt ttaggataat
    20461 aatacttgct gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc atatgccatt
    20521 gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt atacacatca gacacagata
    20581 atcctttttc atctacatcc catgcacctt cacattgatt ggccgctgtc gctccgcccc
    20641 ataaaaatcc ttctggaaat tttttcatat cgatccccct aaacttgcta gttttcttgt
    20701 ttgattattt ttcttatgac tagtaatgat atttttgaat ctactactat ttgtaacaat
    20761 aaccattgtg gtcggatcat atccctcttt gattaattct tgcaaatcta cttccatcaa
    20821 taaatcgcct ttacgtactt tttttccatc agatactgca ctttgaaagt atttgccatt
    20881 taaattcact gtatcaatac cgatatggat cagtatctct gctccttctt cactgactac
    20941 cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag aactgtagat
    21001 ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa gatccactag caaatgttgg
    21061 atcgtttact tcagataaat caattatttc acctgctgca ggagtactta acactatatc
    21121 ctttgaggat tcttcttcga tttcatcctt gtagccgaaa agaaaagtta gcatacagga
    21181 actgatcatc gctaagaaat aactagcaac tagccaaaca aatccttcac caatataaat
    21241 cgggaaagac aaaatacttc ttgtagcaac tgccaaagca ccggctcctg cggcaccagc
    21301 tactccacca aatatagctc cacatataca agcaatgtaa aatggtcgtt tatatttaag
    21361 agtcacacca taaacagcag gttcagtgat tccaaataat gctgtgataa atgcagataa
    21421 agataaactt tttactttct tatttttcgt tttcaaaaat accccaagta ctgctcctgc
    21481 ttgcgcagct acggcagtca tacatgctgg acctagcgta tcacgaccgt agaaagaaag
    21541 attgttggtc attactggta caagtcccca atgtaaccca aaaatcacta acacttgcca
    21601 aaatccgccg ataacagcac cagataaaac agggctaaag ttataaacag ctaaatatcc
    21661 agctgcaatc agctgacttg cataatctgc aattggacct acaactaata aaataattgg
    21721 gaccatcacg atgatacaaa tgaatggtgt caataaattt cgaatacttt cgtgcaagct
    21781 tttgcgaatc ttaggctcaa tataagaaag aacccataca gctaaaattg ctggaataac
    21841 cgtacttgta taccttgcta gaatcacggg catccctaga aatcgcaagg ttatgctatc
    21901 actatatgca ctgacaatcg taggataaac gagagctgct gcaataacca tagcgatgaa
    21961 acgatctgta ttgaactttt ttgcagcagt atatgccaaa aatataggca agaaatagaa
    22021 aaatgcatca gctgctgcat tcaaaatgat ataagttcct gattcagtag ttaataaatt
    22081 caaactcgta cataaggcga gtactccttt gatcaaacca cttcctgcca agacgcctaa
    22141 agtcggggca aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc cttttttctc
    22201 agttcctctc tttccactag atgcatcatc ttctagtttc atttgtttgg ataatacgtc
    22261 atacacttca tttactgcat ttcctactac gacttgaaac attcctccag cttcgactac
    22321 agtgatcaca ccttctagtt gctctacctt ctccttatct gccttcttat tatcttttag
    22381 ggtaaagcgt aaccttgttg cacaatggta aacattgctg acattttctt cgcctccgac
    22441 aaatcggaca atatctttcg ctaattgtga atagtccatt tttacttccc ccttgttaat
    22501 taatgccgta gcaatccttc ttacaagata agtatagttc ttagaaaacg gtttctccat
    22561 tcaattagta ccccttctaa tagcgaacga aactgtacaa tagtgtgtat ggtttactta
    22621 gccgagcaaa tcactcactg cttgaaagaa aacaagtata tcgtttctct tatagtcaaa
    22681 ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt ataagtggta tctcaagctg
    22741 atactctgtg atttttgttt ctacttggct tttgataaga cagcacaaca aagtctcggc
    22801 atttaacgga agcataacta ggtcagaact aggagagatt agttgctgaa tggaatctat
    22861 ctttttatgg atttttattt gattttcttc tttgtagtaa tgatcaagca gttttgtttt
    22921 ctttttttct tcaactatgg tcagcttttc acgcaaaatc tgataaatct cccaagattc
    22981 aaactcatct agaacactag gtagataaat caaatcatcc cactcaaaaa taagctcttg
    23041 ctcagtagtc aaaattgcat catattccgt tattttgttt gattgatttt tgaaaagata
    23101 cactggttct atccggcatt ctttgatcca catgttcata ctttgttgaa tctgtttttt
    23161 tagtgtattc aaaatactaa ttggctggtt actaacaatc agtatctccc cttgttttcg
    23221 aaaaggattc aacgcttcgg caagatacaa aacaaaataa gcgctttcag ctaaattggg
    23281 acgtaattga aaatgtattg ggaagaaacg ccgaattaaa taaatttcta aaggataacg
    23341 ggagatgatc ttttgtacaa aatgattcat ggtattatgt ccggcatcca tcctcgtttt
    23401 catttgtttg atatgggtta gcaaaaattc tgtttcatgg aacaataacg aaacaggaag
    23461 atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga ttcttctcta tttctttgtt
    23521 tttttcatca ttaatcatca aataatttaa ctctaatagt cgattttcaa ttgcttgttt
    23581 ttcttgattg gaaaaagaat agggtagttc tgatgataac ttttttgtga gggtctctac
    23641 catcggaata aataatgtat acctgtttgt tttttctaaa agttcttctc ttcgaag
//
LOCUS       DAZUKA010000026        23663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000026 DAZUKA010000000
VERSION     DAZUKA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.026"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..512
                     /locus_tag="ACG8PN_000850"
     CDS             102..512
                     /locus_tag="ACG8PN_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4861025.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            505..1206
                     /locus_tag="ACG8PN_000851"
     CDS             505..1206
                     /locus_tag="ACG8PN_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4861026.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1206..2792
                     /locus_tag="ACG8PN_000852"
     CDS             1206..2792
                     /locus_tag="ACG8PN_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861027.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2811..3032
                     /locus_tag="ACG8PN_000853"
     CDS             2811..3032
                     /locus_tag="ACG8PN_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4861028.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3029..3790
                     /locus_tag="ACG8PN_000854"
     CDS             3029..3790
                     /locus_tag="ACG8PN_000854"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4861029.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4170..4679
                     /locus_tag="ACG8PN_000855"
     CDS             4170..4679
                     /locus_tag="ACG8PN_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4861030.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4754..5293)
                     /locus_tag="ACG8PN_000856"
     CDS             complement(4754..5293)
                     /locus_tag="ACG8PN_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4861031.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5433..6044)
                     /gene="rpsD"
                     /locus_tag="ACG8PN_000857"
     CDS             complement(5433..6044)
                     /gene="rpsD"
                     /locus_tag="ACG8PN_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4861032.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6306..7157
                     /locus_tag="ACG8PN_000858"
     CDS             6306..7157
                     /locus_tag="ACG8PN_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4861033.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7185..7820
                     /locus_tag="ACG8PN_000859"
     CDS             7185..7820
                     /locus_tag="ACG8PN_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4861034.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7840..8613
                     /locus_tag="ACG8PN_000860"
     CDS             7840..8613
                     /locus_tag="ACG8PN_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4861035.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9021..9818
                     /locus_tag="ACG8PN_000861"
     CDS             9021..9818
                     /locus_tag="ACG8PN_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4861036.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9796..10209
                     /locus_tag="ACG8PN_000862"
     CDS             9796..10209
                     /locus_tag="ACG8PN_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4861037.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10193..12838
                     /gene="mutS"
                     /locus_tag="ACG8PN_000863"
     CDS             10193..12838
                     /gene="mutS"
                     /locus_tag="ACG8PN_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4861038.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12856..14964
                     /gene="mutL"
                     /locus_tag="ACG8PN_000864"
     CDS             12856..14964
                     /gene="mutL"
                     /locus_tag="ACG8PN_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4861039.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14986..15546
                     /locus_tag="ACG8PN_000865"
     CDS             14986..15546
                     /locus_tag="ACG8PN_000865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4861040.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15631..17619)
                     /gene="treP"
                     /locus_tag="ACG8PN_000866"
     CDS             complement(15631..17619)
                     /gene="treP"
                     /locus_tag="ACG8PN_000866"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4861041.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17842..18555
                     /gene="treR"
                     /locus_tag="ACG8PN_000867"
     CDS             17842..18555
                     /gene="treR"
                     /locus_tag="ACG8PN_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4861042.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18632..19078
                     /gene="msrB"
                     /locus_tag="ACG8PN_000868"
     CDS             18632..19078
                     /gene="msrB"
                     /locus_tag="ACG8PN_000868"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4861043.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            19248..19850
                     /gene="ruvA"
                     /locus_tag="ACG8PN_000869"
     CDS             19248..19850
                     /gene="ruvA"
                     /locus_tag="ACG8PN_000869"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4861044.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19863..20864
                     /gene="ruvB"
                     /locus_tag="ACG8PN_000870"
     CDS             19863..20864
                     /gene="ruvB"
                     /locus_tag="ACG8PN_000870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4861045.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20893..21471
                     /locus_tag="ACG8PN_000871"
     CDS             20893..21471
                     /locus_tag="ACG8PN_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4861046.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21523..22005
                     /locus_tag="ACG8PN_000872"
     CDS             21523..22005
                     /locus_tag="ACG8PN_000872"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4861047.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22122..22496
                     /locus_tag="ACG8PN_000873"
     CDS             22122..22496
                     /locus_tag="ACG8PN_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861048.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            22926..>23663
                     /locus_tag="ACG8PN_000874"
     CDS             22926..>23663
                     /locus_tag="ACG8PN_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS4861049.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKY"
BASE COUNT         7736 a         4229 c         5077 g         6621 t
ORIGIN      
        1 gttttttttc tcaaaaattg tatcttagaa acagcatgtt ttgaaacttt cagaaaaggt
       61 ttgttagtaa aatgaaagct atacgtaagg agtgttgttt gatgaaattt tttcaagagt
      121 ttttcattat tattttcttt tctgtattag gagaaggcat acggattttt gctcatttac
      181 ctattcctgg aagtatctta ggtattcttt ttttattttt agcatttgaa atgcatcttg
      241 ttgatcctga aaaaatcgga gcaactggtg attttttatt gaataatcta gccattttat
      301 ttgttcctgc tggtgtaggt ttattggaat attttgatga tattgcttcg atatggcctg
      361 ttttactggg agcagtcgtc gtgtgttcca tcgtcacgat ggttgcagcg ggaaaaacgg
      421 cagaaggtgt agaagctttg ctgggctatg tccgtaagaa aaaagcagaa agggtatctg
      481 taaacgaaat ggaggagaat caaattgact gagttttttg tgaatccatt atttggctta
      541 agtttgactt taggagtgta cgtattatct gacaaatttt tgcggaaata taaactgcct
      601 gttttgaatc ctctcgtatt atcgattgtt gttattatct tatttttgca tttttctggg
      661 atcagctata aagactataa tatcgggggc aattttttga ctatgctgat tactcctgcg
      721 acagtggcac ttgccatccc tctttataaa aacttccgtt tactgaaaga gaatttttat
      781 cctgtaatgg ctgcgatttt agcaggaata gtcgcaaatt gcttggttag tgtagggttc
      841 ggttacttct tctctcttca taaagagatg gtgatctcat tactacctaa atctgtaacg
      901 acggctattt ctgtcgattt atctcatacg atgggcgggg tgaatgccgt gacacttgct
      961 attgttgtct ctacaggtat ttttggttcg ctgatcgcca ctcatatttt ccgcctattc
     1021 aagatcgaaa gtccagttgc tcgcggtgtg gcgttaggaa gtacttctca tgctattggt
     1081 acagcaaaag caatcgagat tggtgaaata gaaggaatca tttccggact tgcgatatgc
     1141 gtcaacggaa tattgaccgt tttattgcta ccgttattct ttcagccttt tgctggcttg
     1201 ttttgatgcg aaaaccacta ttttatgcta caataaccaa aacgttcgag cataaaaaag
     1261 gagggaaaga cgtgtatttg tcttccgagt atcttaaaca atttgataaa gatctatatt
     1321 ataagattct cctcctagaa agttttgagg atcaagcttg gcataccgct gcacaattag
     1381 cgcaagttgt tcagctggat gcccgcagcg tgtcgaaata cctgaacgag ctgtctaaaa
     1441 actatcagca gtttagcggt aagacacacc ctttgtttac aaaaaatcac cgttctggtt
     1501 ataactttta tgatacgctc gattcaattg aacatgagcg ttttttgatc tatctcgtgc
     1561 aaagtacgct gaaattccag ttgctgcacg acattttttt tgaagaattc catacaatgt
     1621 accaatttgc gcaaaagcat tacattagtg aatctactgc tcatcgaaaa atcaatgaat
     1681 ggaaacagca actgcaaact tacggaattc ggctgcaacg aggtacatat atcgctcaag
     1741 gcgaagaaga aatcattcga ctttacttgc atatgacatt ctggcagttg tttcgaggga
     1801 aaatctggcc atttgaaacg atttctcaaa tggacgtaaa gaatatggca gaacatatca
     1861 tggctttttt taatgttcgt ctgaacgaga tcaaaaaaag acgattggaa tatatgctag
     1921 gtgccttttt tttaagaaaa tcacagaagc attatgttgt attgaatgag aaaaaaagac
     1981 ggctgatttc tgacaatctg ttgttccagc gattttgcca ggtcatggag ccggtatttc
     2041 ctaattattt tcaagtagaa gacgagttag gcgcattatt tcttgtactg atgactcgtg
     2101 aagaatatta tagtgatcct aagatacgga agaaaatttt tgattttcat caacaagcaa
     2161 aaacaccgcc ctttacagcc ctttcagaag caaaagccgc tctttctctt tatcaggaag
     2221 agcaggggct gccagcagaa aatctaacct tcgaggcaga aaactattta ttcagcagtc
     2281 attttttcgc ctatcttttt cctaatgcga aagaaacgat tgatggcaat agtagtgatt
     2341 ttattaatca tttagttatt gaaaacaagg aattgaaaca atggctcgta catttttttg
     2401 aaagtagaca caaacatccc aatcatctgg catttaaaaa tcatgcgttt ttgatggggc
     2461 ggtatctgac tgtgtttaaa acattaggag cctttactcc acagcttcca aagatcacga
     2521 tccttttaat gacagatttc ccattatttg aagagcaact gttagaggag ggattacgga
     2581 catttttccg taatgagtat cagctgattt ttctgccaac agattatcgg gggagagaag
     2641 ttgatcttct gatttcgaca agtaaagtcc accgcaaacc atgggcggat cttgactatt
     2701 ttattgtgac tgaagaacta aaattaattg attacatcca actatctcaa aaatttgaaa
     2761 tgattcaaaa acaaaaacta ttaaaacaat aacaaagaag gagaaaaaag atgacatttg
     2821 aagaaatttt accagaattg aaaaaaggtg caaagattat tcgtaaagga tggagtggat
     2881 ttgaacttta tgttgttctc gtatccgatg atgaatttga tggctgtccg gtgacgccgt
     2941 acttattgat caaaacaagt gacgaaggtt tttccagctt cgctcctaca gtttgtgata
     3001 ttttagcaga tgattgggcg atcgtagaat gaaaaaccag tatccagatc tagctaaaca
     3061 ggttgtattt atcacaggag ctgcttctgg aattggtgct gcgcaagccc gagcgtttct
     3121 tgaacaagat gcttttgtat ttggattaga tcaaaagttc ggtaaaatgg aagaaatttc
     3181 tttgcgattt cctgaaactt ttgcctacgc actaggtgat gttcgacaaa tgaaagaact
     3241 ccaacaagcg gtatcgaaat gccaaaatca ttttggtgaa gttacgattt tactgaatac
     3301 agcggggata ttggatgcct acaaaccctt gatggaaact gacgaatcgc tgtgggatct
     3361 gatttatgag acgaatgtga aaagcatgta tcagctgaca aaaatcatcc ttcctgatat
     3421 gatccgccaa aaaagcggaa cgatcatcaa tatggcttcg attgctggct tgattgctgg
     3481 tggaggtggg atcgcttata caagcgcgaa acatgcgatt gtcggattta ccaaacaact
     3541 cgctctggat tacgcaggac agggtatccg agtaaaagga attgctccag gagccatcca
     3601 aacaccaatg aatgcggctg attttgaagg gaaaggtgag atggcagagt gggtggcaaa
     3661 tgaaacacca gtcaagcgat gggcacagcc ggaagaagta gctgagctga cgttatttct
     3721 tgcctctcct caagcaagct acatacaagg aacgatcatc ccaatcgacg gcggatggtt
     3781 gttaaaataa ccttgtgttt ttttcaagct tctgtatgat aaaactaaag agagaaacaa
     3841 cggttgatga taaaaaaagt cgatgactgt tcccgacatt cgatgccgaa cagccagttc
     3901 aaaaaattct ttttaataat cgttaatatt cgaaactaag cggcttttca cgacctcttg
     3961 atataaacga tattcaaaag cttgcaactg cggtaacaag tccagccaaa tgaaaaatat
     4021 aaggagctat ttttatttga ccattcttat tacttgttgc agggcagctt tttcactacc
     4081 ttctgcgaca cttcagttgg aattttcttc ccatcctttg gtcgcggtca ttaccaagat
     4141 aaaattatct tgcaaaggag aaatagaaca tggaaaaaaa taatacccgt tcagccaaac
     4201 gctggacaac gacagatgtg actcggatgg cgctagtcac tggtttatat gtggcagtga
     4261 cgctggtttt gtctgtcttt agctttggag tgattcagat tcgtctctcg gagatgttca
     4321 actatctgtc cttgtataac aaaagataca tcgtcgcggt aacagcggga gtagctttgg
     4381 ctaatattgc ttctcctctt ggactgatag atgttgcagt aggaagcgtc agcacgctgc
     4441 ttgtattgct tatcaactat aaaatcacta gtcgcatcaa aaacatgaaa atcaaaatgg
     4501 ccgtgacagc atgcgtattt gctttttcca tgtttacagt agctggtcag ctaacgattt
     4561 tatatcaact accttttttc ttgaattggt gggtgattgc tttaggagaa ttggcatcaa
     4621 tgattgtcgg tgggataatt atttattgga tcggtaaaaa agtggatttg tcaaaataaa
     4681 gatctgttta atttaaaata gggcagcatg gaagagactg aatgttgagt cttcttccat
     4741 gctgcttttt tgattaattt gtatgaagaa atcgatttag ccgttttttc atcaagatac
     4801 cgatataagc tgctccagtt gccttgattg cttccggcag taaaaacggc agtagtccac
     4861 tggcaaatgc agttgtaaaa gtaagatcac cacttatttt cagccagatc gttccgcaga
     4921 tcaatgcagc aagagagccc attagattgg caaagattgc atataagtaa tgagcatccg
     4981 ttctttcgat catccaacca gttatccacg tttgtaagag aaatccaatc aaatatccgc
     5041 cagttggccc aaataaaaca gctatacctc cagtcatccc tgaaaataca ggaagaccaa
     5101 tcaagccaag taaaagataa actccaacag atatcaatcc tagttttctt ccgagaaaag
     5161 ttgctgttaa tccaatagca aatgtttgcc ctgtcaaagg aaccaaacca aaaggaatgg
     5221 tcatctgaga aaggacagaa ataatcgcgg caaattccgc agcaagtatc aattctttca
     5281 atgttcggtt cataacgccc tcttttcgtt aacttaatat ttttatttgg ttaacgaaat
     5341 tgtaaaacaa agtgattact ttgtaaatag aaaatataaa aaaagaccag ctatccaata
     5401 gatagcggtc tttttcaaga aagcttttga aattataagc tttggttgta gtattcaacg
     5461 acaagagctt cgtcgatttc tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa
     5521 ccttccaatt tttcagcgtc aaagcttacg aatgctgggc gtccaacagt tgcttctaca
     5581 gcttctttta ctgttacgat gttttgtgat ttttcacgaa ctgagatcac ttgacccact
     5641 tctacgtggt atgaagggat atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta
     5701 actaattgac gtgcttgacg acgagtcgtt gcaagtccta aacggtaaac aacattgtct
     5761 aaacgttgtt ctagtaagat catgaagtta acaccatgtt taccttcttt gattttgcta
     5821 gcttttacga acaagttacg gaattgacgt tcgttcattc cgtacatgtg acgaagtttt
     5881 tgtttttcag ttaattgcat gccgtattct gattgtttgc cacggctatt aggtccatgt
     5941 tgacctggtg cgtaagggcg acgtgctaat tcttttcctg tgcctgacaa tgagatgcct
     6001 aagcgacgag aaattttcca tgatggtcct gtataacgag acattaaaat ttcctccaat
     6061 gaatataagt ttttggagta aaataatccg ttgaaaaatt cataatcata tagtttgttc
     6121 ttcaatcttc acctttgcag ccgcggttac acaatcgaac ccgagacggg gcaacaaact
     6181 agggatgagt tattattttt ctgctgcatt attttacaca aattacagta tatgatagat
     6241 catccacgaa tgtcaagata attagtacat aaaaacgctt tacaaaccta ggctgtcttt
     6301 ataaaatgag agggtatgtg caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa
     6361 tgaatccggt atcaccatta tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc
     6421 tttttgaaaa cagcttttca cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga
     6481 ctttaggaac ggctgtggca tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc
     6541 taggaaagat tttatacgca tttatgttca gctggggtct ggtcatgatt ctatacatga
     6601 acgccgaatt aggaacatcg aatatgcttt atatgaccgt gggtgttttt cggcagagac
     6661 ttgctttaag taaggcagcc aagattctat ttgcctgtat ttttttcaat atgattggcg
     6721 gtatattatg tggatttcta atttctatga ctacgccatt tcaagattta acgttggaca
     6781 actttatgtt agaatcaata gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg
     6841 aagggatgtt tgcgaatatc gttgtcaata ccgctgtact gatcagcatg cggatgaaag
     6901 aggatgtcgg aaaagtgatc acagtcgtat tcatcatttt catttttgct tttttaggat
     6961 atgaacacgt gatagcgaat tttcctgcct ttacgcttac ttactttgct tctcacggac
     7021 agatggatgg aatgacagtc agcaatgtgt tacataattt gttttttgct ttggcaggaa
     7081 attatattgg tggcggttta gtaatgggac tgggatatgc ttggctgaat cagtcaaaat
     7141 catcctatgt ggactaaaaa gtcgttcatt aaaggggaag tgccatggaa gttttagttt
     7201 ttccaaaaaa tgaggaaaaa gcgttacaaa tggaaaagta catgaaaaat cttttttcct
     7261 ttgctggagt acctaagccg gaacgcgctc ttttacaaca accgttactg gatgagagca
     7321 aaaatttatc tatttctgaa gttttagctt ttgttcatta ttattatgaa caaatcgaaa
     7381 gagagtatca gtatgtagcg atcgatttag tccagaaaaa catcaaacgg cttacatttg
     7441 aagacttagt cgtattgttg ccactagttc agaaaaaaga atggtgggac agcatcgact
     7501 cttggagaaa agtatacagc acatggtgta aagcctaccc agagcagcgt gaagaagtct
     7561 ttcaattgtt tgaaaaaaag gaagattttt ggctacgtcg aatagcaatc acgttgcaat
     7621 taggttataa acagcaaaca gacacaggac tattgatccg agcaatagac gaagatcgtc
     7681 aaacaaagga attctttatc caaaaagcaa ttggttgggc attacgcgat tatagtaaga
     7741 ccgatgccga ttgggtaaaa agacagctga caacagattt gagtaagtta gctgttcgag
     7801 aggcaagtaa gtatttataa gaaagcgaga cgacaagaga tgagacgcaa attatttgct
     7861 tttgatattg atggaacgtt gttagattca aacaaacaag ctttagatag cacaagagaa
     7921 gcattggaaa aattacgcaa agccggtcat ctggtcacga ttgctacagg gcggagccga
     7981 tttcatgcgc aagagatcat tcgcgatctt gattttacga actacatttt atgtaatgga
     8041 gcggccggtt ttttagacca tgaacaagta tataagaatt tactagatcg cgaccagttg
     8101 gatgcgttcg ttcaagaagc gcatcatgag cagatcgata cagcttttgt cagcttagac
     8161 aatatcaagc gttcttcttc caatgatgta acggtgatgg aagaagcgat gcattctttc
     8221 ggtgctgtct tgcctgaatt agatcagttt tttgcagagg aacaagaaat ataccaagcg
     8281 ttagcatttt tcgatcaaac atatgatggg aagttctctt catatgataa aattcgattt
     8341 gtccgctggc atgagaacag tgtagatgtc gtacctaaaa atggttctaa agcagcgaca
     8401 attctgcact tagcagaaca agtaggcatc cctcatgaag acatcatcag ctttggcgat
     8461 ggacaaaacg accgtgaaat gttgcgcatg tcaggaattg ggatagctat ggggaatgct
     8521 gtgccagaag tacaagcaga agctaagatg gtcactgata caaacgatca ggatggaatt
     8581 tggaaggcat tgaaagagtt aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca
     8641 tcaagtaata agcccagcca aacaaaaata gctcctccta tttttcgttt ttctgggctt
     8701 attaccgcag atgcaagctt tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc
     8761 agtttcaaga aataagaagg gatttacgaa aattgcttct caaatttttg taaaattacg
     8821 gcttatttct gaagaagtag atgaaacaac gtttattcgg gaaatgggac tgtgacaaga
     8881 tgtttgtcat agtcccattt ttttatttta tttttcaaaa ctaagaaata ccaggaaaga
     8941 gttccttttg tttgaccttt tctttcttat tgaaaacaac tataatatag agtgatgtta
     9001 aaagagaaga ggaatttagt gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg
     9061 gacgagagat gattactgaa tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa
     9121 cgattgtaaa tggtgagaat gccgcttctg gacgtggaat cacagaaaaa atctataaaa
     9181 aatttttgca agacggcgta gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg
     9241 gaatctttga gtttgtaaat gaagctaaaa agatggttcg tccggcaaat tttccggaag
     9301 ggacgcctgg acaagggatg gtctttgtca aagtgaacca agtcgaactg gcagtgatca
     9361 acatgcaggc tcgttctttt atggtggact tggatgatcc ttttcgtaaa atgaatgagc
     9421 tggtagaaga agccagaaaa cgtacgccga tcatttttgt agattttcat ggagaaacaa
     9481 caagtgaaaa acaagcaatg ggctggtttt tagatgggaa agtttcagca gtggtcggta
     9541 cacatacaca cgttcagaca aatgatgcgc gaattttgcc aagaggaaca gcgtatctga
     9601 cagacgttgg gatgacaggt ccctatgatg gtattttggg tatgcgcaga gaacctgtaa
     9661 tcgaaaagtt tctgactgca ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga
     9721 ttttaagcgg ctgtatcttg gaattggatg atacgacagg tcatgcaaaa gaaatccagc
     9781 tgattcagat caacgatgat cgaccgttca tggagtagaa agagaggcag ttttttgcag
     9841 caggagccgg aaattcaaaa agaagtaagg aaactgacaa aacttttaag agagaatgaa
     9901 acgatcattc gctacaaaga acttgaagaa aagatccagc aaaaccagta tttagctgag
     9961 ttgagagaaa aaatcaaaca agcgcaaaaa gatgctgttc attttgccca ctatgacaaa
    10021 ccagcagcag aaaaagaagc aatcaaacaa gcggatcaat tcatgcaaga atttgatcag
    10081 catccgcttg ttgtagctta tagaaaacag ctgttggaag cagatgatct gcttcatcat
    10141 ttaacaacta tgatacaaga agaaatcaat ggacaaatag aggaggaaaa acatgcctca
    10201 aaaaactaaa aatactccta tgatggaaca atatctaagc atcaaggccc aataccaaga
    10261 tgcttttttg ttctatcgtt taggagactt ttatgaacta ttttacgaag atgcgatcaa
    10321 agcgtcacag cttttggaat taacgttgac gagcagaaat cgaaatgcag aagaacctat
    10381 tcctatgtgc ggtgtacctc accatgcagc acaaggatat atcgatgcct tgattgaaaa
    10441 aggctataaa gtagcgattt gcgagcaggt agaagatcca aagacgacta aagggatggt
    10501 caagcgcgaa gtgatccagc tagtcactcc tggtacagtc atggacagca aaggattgtc
    10561 ggcaaaagac aacaattttt taacagctgt agttagtcaa ggaaatgaat ttggctttgc
    10621 ttatgccgat ttaagtacgg gagaattgaa gacggctcgg ttgcatgatg aagaagctgt
    10681 tttaaatgaa gcctctgcat tgcagacaaa agaactcgta ttaggcagtg agatcactga
    10741 tacccttcgt gaacagctaa gcgggcgttt agggctagtc ttttctcaac aaaatgagat
    10801 ggaagaaaac gcggaattta gctttttgac aagcgaacta gtcgatccgc tggaaaaaga
    10861 agcaaccggc aaactattga cttatttatc ggtgacacaa aagcggagtt tagctcatat
    10921 ccaaaaagca gtagaatacc aaccagatca ttttttgaaa atggattatt attcaaaatt
    10981 caacctagaa ttaagccaat ctattcggac tgggaaaaag cacggcactc ttctatggtt
    11041 gttagatgaa acaaaaacag caatgggtgg ccgtttacta aaacaatggc tagaccgtcc
    11101 gcttatccag gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt tgctagacag
    11161 ctattttgaa cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc tggaacgcct
    11221 agcaggtcga gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc
    11281 tttagaacaa gtgccaatga tcagagggtt gattgaagga atcgatcaag gtcaatggca
    11341 aagtctgctt tcagaaatgc agccgatgga ccatctcgtc caattgatcg accaagctat
    11401 ccaagatgat ccgccgttac aaataacgga aggaaacatc atcaaagatg gattcaatga
    11461 gcagctcgat atgtaccgtt ctgctatgag aaacggaaag aaatggttgg cagaacttga
    11521 agcaaaagag cgtcaagaaa caggaatcaa gaacttaaaa atcggattta accgcgtttt
    11581 cggttattat atagaaatta caaaagcaaa tcttggaaat gcagaactgg agaagtatga
    11641 acgaaaacaa actttagcaa atgcagagcg tttcatcaca ccagaactaa aagaattgga
    11701 gacacaaatc ctcgaagcag aagaaaaatc tgttgattta gaatatcagt tgtttttagc
    11761 tgtgagagaa gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa aagcaatcag
    11821 tgcagtagat gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt atgttcggcc
    11881 agaacttgtc agtgacaagc atcagcttct gatcaaagac ggacgacatc ctgtagtaga
    11941 aaaagtatta ggacatcagg aatatatccc taacagcgta gaaatggcag aagacgaaat
    12001 gatcttgctg attacaggac cgaatatgtc tggtaaaagt acgtatatgc gacagttggc
    12061 attaactgtt ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag cagttttacc
    12121 tatttttgat cggattttta cccgaattgg tgcaagtgac gacttaattg ctggacaaag
    12181 tacttttatg gtagagatga tggaagccaa tcaagccctg cgttatgcta cgccaaacag
    12241 tttgattttg tttgacgaac tagggcgagg aacagcaacc tatgacggaa tggcgttggc
    12301 ccaagcaatc atcgaatata tccatcgtca tgtacaagca aaaacactct tttctactca
    12361 ttatcatgaa ctgacggtac ttgagaaaga acttccgcag cttaaaaatg tccatgttgg
    12421 agccgttgaa aaagacggag aagttgtctt cttgcataaa atgatggatg gacctgcgga
    12481 taaaagctac ggcatacatg tggcaaagat cgcaggattg ccttccaatc tactagaacg
    12541 agcagcagat atcctgcata cgctagaaaa tggcgataac ggacaccata ctgttccagc
    12601 tgaagtagaa gagaaaacta aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa
    12661 agtaaatcaa gaccatatac aagaccgtgt aaaagaagaa aaccaacagc tttctttatt
    12721 cagtgaatta tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa taaatttact
    12781 agaaatgacg ccaatggatg cactaaacac attatatgaa ttacaaaaac aactgtagga
    12841 agaaggcgga tatcgatggc caaaatccaa gaattatctg aacgtttagc taatcagatc
    12901 gctgcgggag aagtagtgga gcgaccagct tccgttgtga aagaattagt ggaaaatgca
    12961 attgacgccg gcagtactca gattgatatc ttagttgaag aagcgggact gcggacgatc
    13021 caagtgattg ataacggaga aggcattttg gcagacgacg tagagaatgc cttcaagcgg
    13081 catgcgacaa gtaagatcca taatcgcgac gatctttttc gcatacgaac gctgggcttt
    13141 cgaggggaag ctttgccaag tattgcctct gtttctgaaa tgactctgga aacggcgaat
    13201 caggaagaaa aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga
    13261 ccagcaccac tgcgtcaagg aacgaagatt accgtcagca atctgttttt caatacgcca
    13321 gcccgcttga aatacgtgaa aacactgcac acagaattag cgaacatcgg ggacatcgtc
    13381 aatcgactgg cattgagtca tccatcgatt gcttttcgtt tagtacatga tggaaacaaa
    13441 atgctggcaa ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat ctacggatta
    13501 tctaccgcaa aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga gttatttggc
    13561 tatgtttcat tgcctgaggt gactcgtgcc agtaggaatt acttatcgac gatcatcaat
    13621 ggacgtttta tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa
    13681 ttaatggtcg gtcgtttccc tattgctgta ttggaaatca aaatggaccc gcttttagta
    13741 gatgtaaatg ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca
    13801 gagctaatta gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca
    13861 gcggataatc tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa
    13921 atcgatttgt cctacgcttt tgaagaagaa aaacctaaaa agccaagtag tttgaatttt
    13981 gatcgacaga caggtacctt tttcgtagaa gaactaaaga acacatttgt tgacagaatt
    14041 gttgataagt ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga aaacaccact
    14101 tatccagaaa aaacagcgaa aacggtggat aagtttggtg aaaatgttga taactttgta
    14161 gaaaaaacag tggaaaaatc tgaaaagaaa gaatttccca ttgtggataa catggagaaa
    14221 acaaatgaag aacaagcaga taaatccgta gaagaggatg aaaagattta cgaggaagaa
    14281 ttgacactcc atcctgaatt tgatttcact caaggaaacg acaaaacatt agaacaagcc
    14341 atcaagaaat tagaaacgga aaaaccagta caacgttttc ctcagttgga atattttgga
    14401 cagatgcacg gaacgtatct ttttgcccaa agtagcgatg ggttgtacat cattgatcag
    14461 catgctgccc aagagcgcat taagtatgaa tatttccggg aaaaaattgg ggaagtatct
    14521 gatgatctgc aagaattgct tgttccattt gtgttagact atccaaacag tgatgctttg
    14581 aaattaaaag agcaaaaaca cttgctagaa gatgcaggga tttatttaga agaatttggt
    14641 caaaacagct ttattgtacg ggcccatcct acttggtatc ccaatggaga agaagaatcg
    14701 attattcggg agatgatcga tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc
    14761 cgtgaagcaa cagcaatcat gatgagttgc aaacgatcga ttaaagccaa ccattatttg
    14821 aatgaagcac aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa tcctttcaat
    14881 tgtccccatg gccgtccagt attgattcac tttaccaatt cggatatgga aaggatgttc
    14941 aaacgaatcc aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta
    15001 gcatcccaat caccaagaag aaaagagtta ttggctcata tcgtttcaga attcgagatt
    15061 gaacctgctg atatcgatga aacgccattt ccagacgaac agcccgtcga ttatgttcgt
    15121 agaatggctg aagaaaaagc acgaactgtc tgggaacaag atgaaaaaga agaccattta
    15181 gtgattgcca gtgacacgac tgttgttctc aatcaagaga tcatgggtaa accagaagat
    15241 ctggctgagg ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac tgtatataca
    15301 gctgttgttt taaaaacaaa ggaacgagaa gaacggatac tagcagaagc acatgtcact
    15361 ttttatcctt tgacagacga agagatcaaa cgctacttgg agacggggga ttatgcggat
    15421 aaggcagggg catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga
    15481 gattattaca gtatcgtagg atttccgatt ggtgctgtca atcaggcact gaaaacattc
    15541 atttaaacaa aaaagcggac tatctgattt tcgattgaaa atccatgata gcccgttttt
    15601 tttattgaaa agattactta tctactctga ctaagatttt acttcagctt taccgactgc
    15661 ttcgcccggg cgaacaagtc ctgattggtc aagagaaagt cctgcaactt tttcattatt
    15721 ggtcaaagca acaataatgt cagttttctt gccagcttgt tctatttctg gcaaatccat
    15781 ttcagcaatc agggtctctg gtgtgacaag gtatccttct ttcacgaata cattgaaagg
    15841 aacacccttt agttcaacag tatctaatcc catgtgaaca agtacttcca gcccctcatt
    15901 agaaagaata ccaatggcat gttttgtttc aaagacactc gtcactttac cgtttactgg
    15961 tgcatagact ttgccgtttg acggttcgac agcataaccg tcacccatca ttttttgtga
    16021 gaaaactggg tcatttactt cagttatttc cttgatggtc cctgcagcga ctgcgaataa
    16081 tgtttctttt gttgcaactg ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt
    16141 tgctgttgcg ccatttgctg tttgaagttg tacatcagca gcattttcag gaacagcagg
    16201 atcaatctta ttcaaaattc cctgacgtct aaagatgatc gtcaagccaa atggaatgat
    16261 gacagcaatg atcattgcga tgatgaaagg tacccatgtt tctgcttgga tagctagtat
    16321 tcctggcaaa ccgcctactc cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat
    16381 gccagctaat ccagagccga ccattgcagc gacgaaagga tagacgtatt tcaaattgat
    16441 tccgaacata gcaggttcag ttacgcctaa gtaacaagaa atcattgctg gaatcgagat
    16501 ttgttcttct tttttattcc cacgatgtaa gaagatgatg gctaagactg cagagccttg
    16561 ggcgatattt gataaagcaa tcatcggcca taaattcgtt gatccgaaat ctgcaatcag
    16621 ctgcatatcg atggcattgg tcatatggtg gagaccagta atcactaaag gagcatacag
    16681 gaatccgaat acggcactaa acaaccagct gatagaagac gtcaatccag tatttacgat
    16741 atttgagatc cagcttccga ctgtccagcc gataggacct aaaataacgt gtgcagctaa
    16801 aacagttggt aacaatgaaa acaatggaac aaaaatcatc gagatcgact gagggatata
    16861 tttgcggaag aagatttcta gatacgctaa cataaaccct gccagcatgg ctggaataac
    16921 ttgcgcttga taaccaatca tctggacttg agcaaaacca aaatcccaaa atggaatatc
    16981 tgctgcggct gtggaagcta cactataagc atttaataat tgcggagaaa ctaacgtaat
    17041 tcctaaaacg attcctaata tttgtgtagt acccatcttt tttgcaatac tccaagtgat
    17101 acctactgga agaaaatgga aaattgcttc accgggtaac cacaaaaagg cattgacacc
    17161 attccaaaac ttcgagtgtt caacgattgt tccaccaaga ctttcaaact ggataccttc
    17221 aagtacattt ctgaatccaa ggatcaatcc accgacaatg attgcaggaa tcagtggtgt
    17281 aaagatttct gctaaaacgg caatagcacg ttgaacggga tgaaggtttt gttttgcagc
    17341 tgcttttcct tgttctttgg aaaccccttc aactccagat actgcaacga agtcgttata
    17401 aaaagtagaa acatcgtttc ctatgatgac ttggaactgt cctgcattcg tgaacatccc
    17461 ttttacgcta ggtatatctt cgatcgcttt ttcgtccgct tttcctggat cattcaggac
    17521 aaaacgcata cgagtcgcac aatgactaac tgcagcaata ttttcttttc cgccgatatc
    17581 atgtaacagc ttctcggcat ctgcttgata ttttgccata tcaataaacc cctttctctt
    17641 aacttgtcta tgcaagttgt ttcttcattt actatacttt ttttcggaga gaattgcaag
    17701 cgttttatta aaaagtacga gagaaaatga tatcgtttta aaaagcttaa aataagcttt
    17761 tcttaaaaat aggaacattg aaatacaaaa agagctgtag tatagtgaag aaatgatgga
    17821 agaacgagtg agggaaaaaa catgaataag ttcaatgaaa tttttttaga tttggaacag
    17881 aaaattttag cgaaagaata tcctccgcat tcgcttttac ccagtgagaa ccaattgatc
    17941 aaaatttatg gtgtttcacg agaaacaatt agaaaagcgc tgaatctttt gactacggaa
    18001 ggttatattc aaaaaaaaca aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac
    18061 tttcctattt ctggattgac tagctataaa gagctacagg agacccaacg tattccaagt
    18121 gaaacgatca tccatacgtt agaagaaaca gaagtgacaa aagcattgaa cgagatcact
    18181 ggctgggaga tcggtgcgcc tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa
    18241 gtagttatat tagacaccga ttatctatta aaggaaatcg ttcctatttt aactccagca
    18301 caagcacaag gatcgatcta tgaatatttt gaaaatgagc tttctttgac gattgcttac
    18361 gcgcaaaagg agatcacagt agaagaagta acggaaatca tgaaaacagc gatggatctt
    18421 cacgatgaca cttatgcagt agtagtcaga agcctcgttc atctggaaga tacccgctgt
    18481 ttcgagtatt ctgagtcggt tcaccgatta gataaattcc gtttcgttga gtttgcccga
    18541 cgccgaaaag tgtaaatagt tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg
    18601 ctatgatagg atcacaaagg gggcattttt aatggataaa ccaataagac cagaaaaaga
    18661 agaactgaaa gaaaagttat cacccatggc ttatgcagtc acacaagaaa atgcaacaga
    18721 acgaccattt agcgggaaat atgatgattt ttacgaaaag gggatctatg tagatatcgt
    18781 cagcggtgaa ccgttattca gctctgcaga aaaatatgat gcaggttgtg gctggccatc
    18841 attcagcaaa ccaataacga aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat
    18901 gcatcgaata gaagttcgaa gcaaagaagc agattctcat ctcggacatg tgtttaccga
    18961 tggaccagta gatcaaggtg gtctacgtta ctgcatcaat tctgcctcac tgaaattcat
    19021 tccatatgaa aagatggatg aattaggtta tggggtattc aaatcacttg tagaataatg
    19081 gaaatacaca aagagcggta caatacgggg atttactcga tcgtccgctc tttttttatt
    19141 caagcgattg ctatttgaaa gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt
    19201 gctacaataa agggaacgta tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc
    19261 tgacaggtgt ggttaccttt atcaacccct attacatagt agtagaaacc aatggtatcg
    19321 gctaccagat cgcactagga aatccttacc gctacagtag caaactaaat caaaaaataa
    19381 aaatttatgt ccatcaggta atacgtgaag atgcgcatct tctgtatgga tttgattcgc
    19441 tagaagaaaa gcaactgttt ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg
    19501 cccttgcaat catggcaagc gaagaccact ccggtttgat tcaagcagta gaatccagtg
    19561 atgtcactta tttgacgaaa tttccaggag tagggaaaaa gacagcacaa caaatgatct
    19621 tggatctaaa aggaaaattc ggtgagctga gtatcgatac accttttagt ctatttgatg
    19681 aagcaaacac aaaagacgct acagctctat ctgaagcaat ggaagctctc tcggctcttg
    19741 gttacagtga tcgagaaatc aaacgtgtag aaaaacaatt aaaagaagta gataatcaga
    19801 caacggatga gtatctacgc caagcactca agctaatgat gaaaaaataa ggaggagacc
    19861 aaatgacaga tgaatacttg ttatcaccag aatctggcga aaatgaactg agtttggaaa
    19921 aatctcttcg accacagctg ctttctcaat atatcggaca gagaaaagtc aaacaagaac
    19981 ttcagattta catcgaagct gccagaaatc gggaagaacc tttagatcat gttctgttgt
    20041 acgggcctcc tggattagga aaaacgacga tggccatggt cattgctaat gagatgaccg
    20101 taaatattcg tacgacgagt ggccctgcta tcgaacgtcc gggtgactta gtagcaatct
    20161 tgaatgaact ggaagcaggg gatgtcctgt ttatcgatga gatccatcga ttacctagag
    20221 tggtagaaga gatgctttat tcagctatgg aagatttcta tgtagatatc atggtaggac
    20281 aagggccaac cgcacatccg gttcatttcc ctcttcctcc ctttactttg attggcgcga
    20341 cgacacgggc aggtatgctt tctgcacctt tacgagatcg atttggcatc atttctcata
    20401 tggaatatta tgacgaacag gatctgcagg agatcgtttt acgttcttcg gatatttttc
    20461 aaacagagat cattgaagaa ggggcattgg aaattgctcg ccgatcacga ggaacgccta
    20521 gaatcgctaa tcgcttgctc aaacgggttc gtgattttgc gcaagtccaa ggagacggga
    20581 aaatcgatcg tcctatagcg gatcaggcac ttactttgct acaggtcgat caggctggat
    20641 tggattatgt tgaccagaaa ttgttgcgaa caatgatcga gttatatggc ggcggaccag
    20701 tagggttaag tacgctttca gtaaacatag gagaagagcg tgagacggta gaagatatgt
    20761 atgaaccgta tctgatccaa aaaggatttt taaagcgtac acctagaggg cgaattgcta
    20821 ctgcttatgc ctatgaacat tttggttacg actatcatat atgatttttg cttttagatt
    20881 ggaggaaaat gaatgagtga tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt
    20941 gatttgtgcc aaacgcaatc ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca
    21001 aaaatcaatc gacaaacatt ttattatcat tttacagata aatacgattt gcttcgttgg
    21061 gtctattgtg aggatgcatt gcattattta gatccacaat gtctttcact tgataattgg
    21121 gaagagcaag cattgaaaat gctgaaagca attgctgaga agaaagaatt ttatgcaaat
    21181 actgtgcttt ctgacccaga aattttacaa aaagagtttt ctcaactgat ccaaaaatca
    21241 tttatcaaga tttttgaaca aatggatgaa gaaagacaac tttctgcaag agacaaacta
    21301 ttttatgctc gttttttttc ttacggttgc agtggtgtat tagtcaattg gatcacgaat
    21361 gaatatgaag aaacaccgat ggaaatcgct atgcagcttt tccgtttagc caaggatacg
    21421 gaattatatt cttatcgtct ttatgcaaaa gaagagttgg aagaacagta atagaaaaat
    21481 tttttgttgg aaaagcaata atcgctaaaa ggagatgggg aaatgacaaa aattttattt
    21541 gtttgtttgg gaaatatttg ccgttcaccg atggcagaag gcttgctgag aaaacgaata
    21601 gcattagaag gaagggaagc agaatttttt gtggattctg ctgctacaag cacgtatgaa
    21661 gttggaaaga caccgcaccc tggaacgaaa aaaatattaa accaagaaaa agtagatatg
    21721 acgaatatgg ttgctcggca aatcacaccc cacgattttg aaacttttga ttggatcatt
    21781 ggcatggatc aagaaaatgt agaagaactg aaacgaagag caccacgctc agcgcagggg
    21841 aaaatccatt tgtttttaag tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca
    21901 tactatacca ataactttga tgagacatat cacttgatca cagaaggcct gaatcattgg
    21961 tggaatattt ggattgatga aaataaaata acaaacaatt tgtaaagtta acgctttctt
    22021 tttaaggcga tgttagagca tttccttgtt tttttgtaaa atgttaggta aaataaaatt
    22081 aattaagtaa cactttctta gctaaaggag gagtgcattc gatgaacgaa gagacagtat
    22141 cgccaatgaa gaagcttacg aagttagtta ctcatacttc gtatcaactt tttgattctt
    22201 ttgtaaatga cactgaagaa catgctaaga atgtacgatt gcaaggaaag tttattcttg
    22261 agctgcaaaa agctgctact gaacatagtc tagttatctt acaagtaaga gcaaatcgtt
    22321 tgagtagcaa gtatgaaacg atctcaggct gggtggcgac aaaaacggtc gaaaaagacc
    22381 gagtgatgat ccgcctacaa aacgaagagc agcagatccg gatggttcct gtagaacaaa
    22441 ttgaaaaaat aacaatcctt tcaccagcag gggaacagga acggttatca aaataattta
    22501 taccagatag aaaagcacgt atgagcttgt accatcaatc attactaatc aaaaataacc
    22561 gaatgcttta agctgatatc agtaaaacgg aacgttttag gatgagtatc atagcatgac
    22621 agcattcggt tgattagttg aatcgattgg atggtacaag tttttttgat aaaaaaaggt
    22681 cctgctagcc ggggagctag caggataagg agttaaaaat gaaaaagtgt tgttagggtt
    22741 gtttgttggt atgcttatat aatagtcatt agttgtgaat attttgtgac gaaagttttc
    22801 gaaagttgtt aagtttttga gagtataaaa tattttgtgt aaatgaaaaa atccatacaa
    22861 aaaaggaagt cccttctgta gaataaagtt aacgacaacc aattcacaga aaagaggact
    22921 tccctatgaa tgattttact acagaaattg tgcaaactct agtcactaaa ggcgatttaa
    22981 atgaattatt ccgttcgcac ttagaaaaag cgataaacac actcctacgg actgaattaa
    23041 cggctttttt agattacgaa aaatatgatc gcactggttt taattcaggt aattcgagaa
    23101 acggttctta ctttcgatca atcaaaaccg aatatggtga attaacattg gaaataccta
    23161 gagatcgtaa tggtgagttt aaacaacaaa ctttaccagc ctacaaaaga acaaacgata
    23221 cattggaaac cactattatc catttattcg aaaaaggtgt tacgatgtct gaaattgctg
    23281 atttgatcga aaaaatgtac ggtcatcact atactccaca aaccatgtcc aacatgacta
    23341 aagttctgac tgaagaagta aatgccttta aatccagagc cttaaatgat aagtatgtcg
    23401 ctatttttat ggacgctact tacattccac taaaacgtca aaccgtatcc aaagaagcga
    23461 tttatattgc cattggtata cgagaagacg gcactaaaga agtactgagt tatgcgattg
    23521 ctccaactga atcaacatac gtttggaatg agctgctaca ggatattaac tccagaggag
    23581 ttcaagaagt cttgcttttt attacggacg gcttaaaagg catgaaagat actatccatc
    23641 aaatttatcc taaagcaaaa tat
//
LOCUS       DAZUKA010000027        23616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000027 DAZUKA010000000
VERSION     DAZUKA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.027"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1412
                     /locus_tag="ACG8PN_000875"
     CDS             72..1412
                     /locus_tag="ACG8PN_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4861050.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1630..2073
                     /locus_tag="ACG8PN_000876"
     CDS             1630..2073
                     /locus_tag="ACG8PN_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4861051.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="ACG8PN_000877"
     CDS             complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="ACG8PN_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4861052.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4398..5621)
                     /locus_tag="ACG8PN_000878"
     CDS             complement(4398..5621)
                     /locus_tag="ACG8PN_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4861053.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5652..6491)
                     /locus_tag="ACG8PN_000879"
     CDS             complement(5652..6491)
                     /locus_tag="ACG8PN_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4861054.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6508..7188)
                     /locus_tag="ACG8PN_000880"
     CDS             complement(6508..7188)
                     /locus_tag="ACG8PN_000880"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4861055.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7190..8263)
                     /locus_tag="ACG8PN_000881"
     CDS             complement(7190..8263)
                     /locus_tag="ACG8PN_000881"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4861056.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8319..8557)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8600..8833)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8910..9581)
                     /locus_tag="ACG8PN_000882"
     CDS             complement(8910..9581)
                     /locus_tag="ACG8PN_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4861057.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9668..12316)
                     /gene="mgtA"
                     /locus_tag="ACG8PN_000883"
     CDS             complement(9668..12316)
                     /gene="mgtA"
                     /locus_tag="ACG8PN_000883"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4861058.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12658..12832)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13005..13442
                     /gene="ndk"
                     /locus_tag="ACG8PN_000884"
     CDS             13005..13442
                     /gene="ndk"
                     /locus_tag="ACG8PN_000884"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4861059.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13464..14330)
                     /locus_tag="ACG8PN_000885"
     CDS             complement(13464..14330)
                     /locus_tag="ACG8PN_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4861060.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14490..16583)
                     /locus_tag="ACG8PN_000886"
     CDS             complement(14490..16583)
                     /locus_tag="ACG8PN_000886"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4861061.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17332..17955)
                     /locus_tag="ACG8PN_000887"
     CDS             complement(17332..17955)
                     /locus_tag="ACG8PN_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861062.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18114..19511)
                     /locus_tag="ACG8PN_000888"
     CDS             complement(18114..19511)
                     /locus_tag="ACG8PN_000888"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4861063.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19543..20514)
                     /locus_tag="ACG8PN_000889"
     CDS             complement(19543..20514)
                     /locus_tag="ACG8PN_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861064.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20888..21313
                     /locus_tag="ACG8PN_000890"
     CDS             20888..21313
                     /locus_tag="ACG8PN_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861065.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21801..22502)
                     /locus_tag="ACG8PN_000891"
     CDS             complement(21801..22502)
                     /locus_tag="ACG8PN_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4861066.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22518..22958)
                     /locus_tag="ACG8PN_000892"
     CDS             complement(22518..22958)
                     /locus_tag="ACG8PN_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4861067.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22958..23533)
                     /locus_tag="ACG8PN_000893"
     CDS             complement(22958..23533)
                     /locus_tag="ACG8PN_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4861068.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6792 a         4897 c         4061 g         7866 t
ORIGIN      
        1 gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa
       61 agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca
      121 tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg
      181 ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg
      241 tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg
      301 gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt
      361 ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt
      421 ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca
      481 tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa
      541 tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg
      601 gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg
      661 gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac
      721 tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca
      781 aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc
      841 tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa
      901 aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct
      961 catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc
     1021 gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg
     1081 ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca
     1141 aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta
     1201 tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca
     1261 gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca
     1321 ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa
     1381 agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat
     1441 gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt
     1501 tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa
     1561 aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt
     1621 ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt
     1681 atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca
     1741 cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg
     1801 tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt
     1861 tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg
     1921 aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa
     1981 gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac
     2041 aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag
     2101 gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga
     2161 ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2221 cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     2281 ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa
     2341 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2401 tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa
     2461 tggctttcca catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc
     2521 gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat
     2581 tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt
     2641 ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg
     2701 aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt
     2761 ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca
     2821 gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca
     2881 gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa
     2941 atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag
     3001 ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc
     3061 atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt
     3121 gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga
     3181 ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc
     3241 tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta
     3301 aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt
     3361 gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac
     3421 atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt
     3481 gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca
     3541 accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt
     3601 gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca
     3661 gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg
     3721 atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg
     3781 atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt
     3841 tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag
     3901 acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt
     3961 gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt
     4021 cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct
     4081 cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc
     4141 ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc
     4201 atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac
     4261 acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt
     4321 tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca
     4381 cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct
     4441 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
     4501 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
     4561 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
     4621 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
     4681 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
     4741 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
     4801 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
     4861 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
     4921 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
     4981 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
     5041 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
     5101 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
     5161 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
     5221 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat
     5281 tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
     5341 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta
     5401 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     5461 aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     5521 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     5581 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     5641 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     5701 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     5761 gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     5821 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     5881 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     5941 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     6001 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     6061 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     6121 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     6181 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     6241 aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg
     6301 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     6361 tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt
     6421 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     6481 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     6541 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     6601 tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac
     6661 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     6721 gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc
     6781 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     6841 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     6901 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     6961 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     7021 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc
     7081 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     7141 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     7201 cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac
     7261 cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa
     7321 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     7381 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     7441 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     7501 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     7561 cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt
     7621 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     7681 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     7741 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     7801 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     7861 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     7921 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     7981 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     8041 tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     8101 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     8161 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     8221 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     8281 taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg
     8341 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     8401 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     8461 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     8521 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta
     8581 ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt
     8641 ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa
     8701 cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct
     8761 tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt
     8821 tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa
     8881 gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt taacatttca
     8941 ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg
     9001 gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa
     9061 gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct
     9121 gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct
     9181 ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct
     9241 cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc
     9301 aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa
     9361 tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact
     9421 aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg
     9481 atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg
     9541 tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc
     9601 aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc
     9661 ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa
     9721 gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca
     9781 tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta
     9841 atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg
     9901 tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat
     9961 ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca
    10021 ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac
    10081 ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct
    10141 cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga
    10201 tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg
    10261 caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca
    10321 tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt
    10381 tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa
    10441 taccgatatc agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga
    10501 cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt
    10561 ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga
    10621 ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg
    10681 taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt
    10741 tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa
    10801 ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg
    10861 cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt
    10921 cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt
    10981 tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa
    11041 ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat
    11101 tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat
    11161 ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt
    11221 catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg
    11281 ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga
    11341 tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca
    11401 ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta
    11461 gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa
    11521 atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt
    11581 tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga
    11641 ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg
    11701 tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc
    11761 ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg
    11821 gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa
    11881 tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt
    11941 tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa
    12001 ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat
    12061 attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt
    12121 atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac
    12181 cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga
    12241 atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat
    12301 ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct
    12361 tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga
    12421 tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt
    12481 gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt
    12541 tatttttcca attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa
    12601 aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct
    12661 cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc
    12721 taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt
    12781 ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc
    12841 cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac
    12901 tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc
    12961 acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg
    13021 ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg
    13081 aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa
    13141 aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga
    13201 cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta
    13261 agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg
    13321 cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag
    13381 agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt
    13441 gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc
    13501 tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg
    13561 tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc
    13621 atcaatgttt catagatcac tgtacttctt tctttatgtg ataagctccg tttcatatct
    13681 tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc
    13741 attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt
    13801 tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca
    13861 gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg
    13921 attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc
    13981 atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta
    14041 ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt
    14101 tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt
    14161 gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa
    14221 tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt
    14281 gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc
    14341 ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac
    14401 gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt
    14461 gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct
    14521 gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga aaagacactt
    14581 gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag
    14641 tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt
    14701 tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag
    14761 atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa
    14821 gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact
    14881 acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg
    14941 attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg
    15001 actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg
    15061 ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta
    15121 tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata
    15181 ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt
    15241 cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg
    15301 tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt
    15361 tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa
    15421 cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag
    15481 tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt
    15541 gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca
    15601 agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca
    15661 gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct
    15721 tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta
    15781 atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc
    15841 ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca
    15901 atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca
    15961 taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc
    16021 tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct
    16081 gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt
    16141 ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg
    16201 acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa
    16261 aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc
    16321 aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt
    16381 ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc
    16441 cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca
    16501 atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg
    16561 cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt
    16621 tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt
    16681 ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac
    16741 taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt
    16801 tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc
    16861 ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct
    16921 cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt
    16981 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    17041 ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa
    17101 agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa
    17161 attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    17221 aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    17281 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc
    17341 ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc
    17401 acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt
    17461 tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa
    17521 acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt
    17581 tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc
    17641 tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg
    17701 attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt
    17761 tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc
    17821 tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga
    17881 aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg
    17941 tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct attatataca
    18001 aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga acttcttttt
    18061 cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct ttcttaagcc
    18121 tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt gattgttctt
    18181 tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata tgcattggtc
    18241 cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc accttcttga
    18301 atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata atcatcgtgg
    18361 atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc gttttcagaa
    18421 ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc atagattcct
    18481 tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat ttcataattg
    18541 tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc ttttgctttt
    18601 acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc tttgataacc
    18661 gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat ctcaatcaat
    18721 tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa gaacgcatct
    18781 gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata gcttggtgtc
    18841 agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga tttgatggct
    18901 aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat atctgactct
    18961 tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg ttcattatga
    19021 gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc atatttttca
    19081 taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata catcggcata
    19141 tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc atcaaaataa
    19201 cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg tatcaaacgg
    19261 ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc
    19321 tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt
    19381 tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc gcctgaaatc
    19441 cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc tggaaattga
    19501 tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga ttccatgtat
    19561 gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga ttcgttttaa
    19621 cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg tgattttcgg
    19681 aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa tatccttccc
    19741 gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat tgtaattgct
    19801 gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat agccgctttt
    19861 tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt ggaccgcttc
    19921 tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag caataaatcc
    19981 gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac tttctcgatc
    20041 attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg cgataactgg
    20101 aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata agaagacaat
    20161 cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt tttctggctg
    20221 atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc tttcgatccc
    20281 tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc caatagagaa
    20341 gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca acgctgaaat
    20401 cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca agtaacggga
    20461 aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg ccatacattc
    20521 tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga tgaccgcatt
    20581 ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag tagagcaact
    20641 tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt taatatagca
    20701 tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg gattttttgc
    20761 ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc gtttttctct
    20821 tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat caaatggggg
    20881 aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt tgacattgac
    20941 tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg attcttctgt
    21001 cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag tacgtaaaga
    21061 tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat ggacacgcac
    21121 gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag tgctgacaga
    21181 tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa ataaaaaaga
    21241 aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg atggcaacat
    21301 ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct tttctgctat
    21361 agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt cctgattatt
    21421 gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga agcttgagca
    21481 tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa tcttatcctg
    21541 ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact cactatggct
    21601 gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca gtaaaacaaa
    21661 cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc aaatatttga
    21721 atataaaata accaaacgat agaaaggctt attctacatc acgtagtaat ttttctttcc
    21781 cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg gttatccgta
    21841 aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat ctgacggatc
    21901 accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc cacttgtatt
    21961 ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa cgtttcataa
    22021 taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg ttttttgata
    22081 cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg ccgttttttt
    22141 ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta ctgctgtact
    22201 tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct atcttcatag
    22261 atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc taaaaatcgc
    22321 ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga ataaaagagc
    22381 ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat ctcttttgca
    22441 agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa aatcactgac
    22501 atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg caatgtttca
    22561 aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact ttgtgtaatc
    22621 atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt caacatagtt
    22681 gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa gtcaaggata
    22741 gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac ttcctgcgcc
    22801 catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga gtagaaactt
    22861 gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc gatttgctcc
    22921 tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc ttcaataata
    22981 actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga ttgttggaaa
    23041 agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa gtttctaata
    23101 attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc ttgtcgggcg
    23161 tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta cctagacctc
    23221 cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt tcatcgatcg
    23281 tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca aaaagcccat
    23341 tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta atcactccca
    23401 taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc
    23461 ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa
    23521 cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt
    23581 ttttttaaaa taaaagacta gattttgtag tttttt
//
LOCUS       DAZUKA010000028        23467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000028 DAZUKA010000000
VERSION     DAZUKA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.028"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            224..1552
                     /locus_tag="ACG8PN_000894"
     CDS             224..1552
                     /locus_tag="ACG8PN_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4861069.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            1452..2207
                     /locus_tag="ACG8PN_000895"
     CDS             1452..2207
                     /locus_tag="ACG8PN_000895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4861070.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            2211..3422
                     /locus_tag="ACG8PN_000896"
     CDS             2211..3422
                     /locus_tag="ACG8PN_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4861071.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            3433..3744
                     /locus_tag="ACG8PN_000897"
     CDS             3433..3744
                     /locus_tag="ACG8PN_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4861072.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            3741..4079
                     /locus_tag="ACG8PN_000898"
     CDS             3741..4079
                     /locus_tag="ACG8PN_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4861073.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            4072..4503
                     /locus_tag="ACG8PN_000899"
     CDS             4072..4503
                     /locus_tag="ACG8PN_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4861074.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            4500..4841
                     /locus_tag="ACG8PN_000900"
     CDS             4500..4841
                     /locus_tag="ACG8PN_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861075.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            4844..5434
                     /locus_tag="ACG8PN_000901"
     CDS             4844..5434
                     /locus_tag="ACG8PN_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4861076.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            5444..5824
                     /locus_tag="ACG8PN_000902"
     CDS             5444..5824
                     /locus_tag="ACG8PN_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861077.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            6022..8592
                     /locus_tag="ACG8PN_000903"
     CDS             6022..8592
                     /locus_tag="ACG8PN_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4861078.1"
                     /translation="MIYLADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSE
                     FDKNDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLN
                     NAKAQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKF
                     GGVLKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNA
                     DNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDI
                     ESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEA
                     IHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQF
                     QNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTL
                     IPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNG
                     LIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQ
                     LLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIIT
                     TIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLA
                     NAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPST
                     LFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSM
                     LDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY
                     "
     gene            8663..9064
                     /locus_tag="ACG8PN_000904"
     CDS             8663..9064
                     /locus_tag="ACG8PN_000904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861079.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            9141..9833
                     /locus_tag="ACG8PN_000905"
     CDS             9141..9833
                     /locus_tag="ACG8PN_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4861080.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            9830..13720
                     /locus_tag="ACG8PN_000906"
     CDS             9830..13720
                     /locus_tag="ACG8PN_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4861081.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            13730..13897
                     /locus_tag="ACG8PN_000907"
     CDS             13730..13897
                     /locus_tag="ACG8PN_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861082.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            13884..14291
                     /locus_tag="ACG8PN_000908"
     CDS             13884..14291
                     /locus_tag="ACG8PN_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFS4861083.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            14292..14711
                     /locus_tag="ACG8PN_000909"
     CDS             14292..14711
                     /locus_tag="ACG8PN_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFS4861084.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            14701..15609
                     /locus_tag="ACG8PN_000910"
     CDS             14701..15609
                     /locus_tag="ACG8PN_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4861085.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            15725..16762
                     /locus_tag="ACG8PN_000911"
     CDS             15725..16762
                     /locus_tag="ACG8PN_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFS4861086.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(16823..17128)
                     /locus_tag="ACG8PN_000912"
     CDS             complement(16823..17128)
                     /locus_tag="ACG8PN_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861087.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            17423..17596
                     /locus_tag="ACG8PN_000913"
     CDS             17423..17596
                     /locus_tag="ACG8PN_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFS4861088.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            17657..19210
                     /locus_tag="ACG8PN_000914"
     CDS             17657..19210
                     /locus_tag="ACG8PN_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4861089.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            19258..19623
                     /locus_tag="ACG8PN_000915"
     CDS             19258..19623
                     /locus_tag="ACG8PN_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4861090.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            19625..21175
                     /locus_tag="ACG8PN_000916"
     CDS             19625..21175
                     /locus_tag="ACG8PN_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4861091.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            21298..>23467
                     /locus_tag="ACG8PN_000917"
     CDS             21298..>23467
                     /locus_tag="ACG8PN_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861092.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVG"
BASE COUNT         7523 a         4200 c         5361 g         6383 t
ORIGIN      
        1 tgacgtttaa aaaacttgca ccaagtagtt cattctttcc ttatcaaaga aaatccttaa
       61 cacctgacga aaaatcgaaa tgtttagagg aagtaaatac ctttttaaat aatgcaattg
      121 agataatttt gtcaagagga agtcggtttt atatctaatg tgaacacagc atctacaaat
      181 cgtaggtgct tttttgatac tcaaattttg aaaggagcaa tcaatgggca taataaaatc
      241 actattcaaa tcacgcgata aaccaacaaa tcaaaccatc atgaatccat accgatttat
      301 gtttggcggc tcatcagctg gtaaaagcgt gaccgaacaa acagctatgc agatgacggc
      361 tgtgtactca tgtgtgcgcg tgctatcaga agccgtggca ggattgccat tacactttta
      421 ccaataccga gatgctggcg gcaaagataa agcaatgaaa cattcgttgt atttcttgct
      481 gcatgatgaa ccaaattcgg aaatgacatc atttgtattt cgtgaaacct taatgactca
      541 cctgctactt tggggtaacg cctatgcgca aattatccga aatggcaaag gtgaagttgt
      601 agctttgtat cctttaatgc cagataagat gacggttaat cgcgatgaca acaaggaaat
      661 ctattattta tatcaagctg atgtcgggca agtcagactt tcaaaagctg acgtgcttca
      721 cattcctggc cttggctttg atggcttggt tggttattca ccaattgcca tggctaagaa
      781 tgctatcggg cttgcgattg caacagagga atatggttct aaattttttg ccaatggtgc
      841 caatcctggt ggtgtcctag aacatcctgg aactttgaaa gacccaggaa agcttcgtga
      901 aagttggaat gcgacatttg gtggcagttc aaatgcggcc aaggttgcag tacttgaaga
      961 aggcatgaag tatacgccta tttccatgtc accagaacaa gcccagttcc ttgaaacacg
     1021 taagtttcaa ctggaagaaa tcgcacggat attccgagtg ccccttcaca tggttggcga
     1081 cttagaccac gcgaccttct caaacgttga acatttgagt ttggattttg tgaagttctc
     1141 attatcacca tggctcaatc gttgggaaca agcacttaat aaatcactgt taagtcgcga
     1201 tgaaaaagaa aagtatttca taaagtttaa tgtggatggc ttattgcgtg gtgactatgc
     1261 cacacgtcaa caaggctatg caaccggtat tcaaaatggg ttcctatctc ctgatgatgt
     1321 gcgtgaactt gaggacttga acccaatccc tggtggtgct ggtgcgaaat attacttcaa
     1381 tggcaatatg ctgccaattg aacaagccgg ggcagcttat ggcgtaaaca aagaagaaag
     1441 cgaggaacaa gatgaagaag ttttggaagt ggaagaatca aacggttctg aatcaagaca
     1501 accaagagga aatcctagag agaaccctct acctcaacgg accaatcgct gaggagagct
     1561 ggtttgatga tgaagtcaca ccgcagattt tcaaagatga gttaaattca ggtggaggta
     1621 acgttacagt ctggattaat tcgggtggtg gcgattgtgt cgctgccgca agtatttaca
     1681 acatgcttgc tgactacaaa ggtaaagtca cagtcaagat tgacggcatc gccgcttcgg
     1741 ccgcaagtgt tattgcaatg gcaggtgaca aggtactcat gagtcctgtg gcgatggtga
     1801 tgattcacaa tccgtcagcg gttgcgtttg gcgaaaaagt tgatatggag aaagccattg
     1861 ccatgcttga tgaagtgaaa gcaagtatta tcaacgccta tgaacgtaaa acgaatttat
     1921 cgcgtgtgca actggcacat atgatggatg cggagacgtg gatggacgca aataaagctg
     1981 ttcacctagg atttgccgat gaaatcatcg gtgagattga cccggatatt ccggttaata
     2041 attcgatgta ctcggaagtc aaagtaacta attcactgac ttcagcgatt gctaagaagt
     2101 gtcgtattga accacctgaa ccaaaggaac gagaagaaat taataacact atcccagccg
     2161 attcgttaat gagtcggctt aatttaatga aaaattggag gaactaaccc atgaacaaaa
     2221 ttcaagaatt gatggaaaaa cgtgcacaag cttggaacgg agcaaaggct tttgtagaag
     2281 caaagcaaga taaagatggc ttgatgtctg aagaagatgc caagacttat gcggaaatgg
     2341 aaacaaaagt gaagaacttt acgatggaaa ttgaacgtat gcagtcaatg gaagcgatgg
     2401 atcgtgaact tgaaaagcca atgagccaac cattaacttc gcaacctatg actactgaaa
     2461 ataaaatgga acctaagaaa acaggacgtg cttcagctga atataaagaa ggtgtacttg
     2521 aagccttgcg ctcaaacttc cgacatgttt caaatgttct tcaagaaggt gtggatacgg
     2581 caggcggtta tttggtgcca gaagaatatg actcacgttt gattgacggc atgacttctg
     2641 aaaacatcat gcgtacactt gcgacaacaa ttacgacaag tggtgaacac aagattaata
     2701 ttgcaggttc taaaccagcc gcttcatgga ttgaagaagg tggcgagttg agctttgggg
     2761 atgcaacgtt cgatcaaatc ttgcttgatg ctcacaaact tcatgtggca atcaaggtga
     2821 ctgatgaatt gctttatgac aacgccttta accttgaaaa ctatatcatc gaccaattca
     2881 ccaaagcctt agcaaacgcg gaagaagatg cgttcttgaa tggtgacggt gttggtaaac
     2941 cactcggtat ttttgcagca acaggtggcg gacaagttgc cattacaact aatactcaaa
     3001 gtgcagttac ggcagatgat gtggtgaacc ttgtgtatgc tttgaaacgt ccatatcgta
     3061 aaaacgcgaa gtttatcatg aacgaccaaa ccatcagctt gcttcgtaag ttgaaagaca
     3121 acaatggtgc ctacatgtgg cagccagctt tggtagcagg tgaacctgac cgtctgctcg
     3181 gttatccagt ttatacttcg gagtttgttc caaccgttaa ggcaggtgcg ccagtcattg
     3241 cttttggtga tttcagctac tataacattg gcgaccgtgg cgtgcgttca ttcgaccagt
     3301 tgcgtgaact ctttgcaggt aatggcatgg ttggtttctt agctaaagaa cgtgtggacg
     3361 gtaaacttgt gttacctgaa gcagtgcaaa tcttgaagat gaaagcatct gcctcatctt
     3421 aaggagtagc ctatgagtga cttactaaat aaggtcaagg caaacctcat acttgagcat
     3481 aacgaagatg atgagctgtt ggaacgattg attctgaccg ctctttctta tgctgagagc
     3541 tatcaacata ttgagtctgg ctactattca gaacatgaaa tgccgccaac cactgaacag
     3601 gcggttattg tgttagccag taacttttac gaaagccgtg atggttcaac tggtggcttc
     3661 tttgcggata acgtcaatgc cagtaagcaa gtctgggaaa cggtcaatct gttactcagg
     3721 ctcgataggc ggtggcaagt atgagttttg gaaagatgaa tcaacgaata caaatcgtta
     3781 aagtgacgaa ccaaaaggat agcgctggat tttcagtgaa acaagatgaa gttcttgcag
     3841 ctgtccatgc atacaaagag gaaaagaatg cgactgagaa atgggtaaac atggcgactt
     3901 ttagttcagc gacctgcctg tttcggattc gttatattcc taatctagag gttacgacag
     3961 agatggttgt catttcagat cagaaaaggt atcagattgt gtcggtggag aatgtccgaa
     4021 atcgcaatat gtaccttgaa atgctgacta ctgaaatgga ggtgagtgcg gatggctaaa
     4081 gcattgatga agatgccaga agatttcttg ttaaaggttt caaaactaga aaacaagaca
     4141 gatgaaatca tccccaaggt tttagaaagt ggtgctgaag tggtggaagg caaagtccga
     4201 agtaacttaa ctgccgttgt tggctcaaat acaaaagtac caagtcgttc aactggtgaa
     4261 cttgaatcag cccttggtat cacacaagct cgtcaagata aaagcggcaa ctggaacatc
     4321 aaggttggtt ttgatgagcc tcgaatcgat ggcagttcaa atgctaagat tgcgaacatt
     4381 ctcgaatatg gaaaacacgg tcagccagca aagccattct tgaaacctgc aaaatcacaa
     4441 agtcgcaagt catgcattga aacaatgaaa tcaaaaatgg aaagtgaggt aaacggcatt
     4501 tgaacatttt agaggaattg aacagtcttt ttgccacact tcatattcct gttgaaacgg
     4561 gcgagtttag tgataaagcg ccagacaagt attgtgtgct gacaccactc gctgacaggt
     4621 tcgatgttta cggggataac tttccaaccg ttgatgtaca ggaagttcgc atttccttgt
     4681 ttgtgaaagg caactatctg aacacgcaaa aacaactggt tcagaacttg ttgaaacacg
     4741 agtttacgat tactgaacga ttatttgtag gatttgaaaa agatacaaat tactatcatg
     4801 tggcgattga tgtcgccaag cattatgaaa tggaggtctg aaaatggcaa cgattggatt
     4861 ggataaatta tattatgcac cgattacaga gtcggcaact actggcgaag aaacatacgg
     4921 cacacccgct atgcttgcga aagcaatctc agctgagctt tcggtggaac ttgctgaagc
     4981 aactttatac gccgatgatg gtgcatcaga agtagtgaaa gaatttaaaa atggaaagtt
     5041 aacgctcggt gttgatgata ttggacgtgc tgtggcggca gtgcttgttg gtgcaaccgt
     5101 ggatcaaaac ggtgtcttaa tttcgacaac cgaagatggt ggcacaccag ttgcgattgg
     5161 tttccgtgct cgtaaatcga atggcaaata caaatatttg tggttgtacc gagtgaagtt
     5221 tgccgtgccc agcacaagcc tagcaacaaa aggtgacgac attacattct caacaccaac
     5281 aattgagggt acagtcatgc gtcgtaacaa gttagatgct agtggtaagc atccatggaa
     5341 agcagaagtt gatgaaaacg atacgggagt tgaacaaaca actatcaatg gatggtacac
     5401 gagtgtttat gaaccagatt tcacagcaga ctaggaggca acaatgagtg aagaacgcag
     5461 tgccaagatt agccttggcg gagatgaata tgaactgatt ttgacaacaa aagccacgaa
     5521 agaaatcgcc agtcactacg gtgggcttga taaccttggt gagaaattgt tgaaatccga
     5581 gaactttgaa ttggcgcttg atgaaatcat ctggttgatt acgctgttgg cgaatcagtc
     5641 catcaagatt tacaacttga agaacacaga aaagaaagat gaactcacga ctgaatatgt
     5701 ggagttgatg acgagtccgt tgcagttggc tgaatataaa tcagcaatca ctgaagcaat
     5761 gttcaagggc acagctcgca atatcgaaag tgaaacagat tcaaaaaaca aagcaggcga
     5821 gtaagtgatg aagaaacatt tactcgcctg tattactatg ggacagttca aatgggtatg
     5881 acgtcagatg atttctggta ctgctcgctt ggactgtttt tggatttatg ggagtgtcat
     5941 aagcaattta ctgggattag caagccgaaa gttgagttat tcattgatga tgtgataccg
     6001 agtggaattt aagggaaggt ggtgatttat ttggcagata attttggttt aaagattggc
     6061 gttgaaggtg aaaaggagtt taagaactcg ttgcgtgaaa tcaaccaatc attcaaggtg
     6121 ctaggttcag aaatgaaact ggtatcatcc gaatttgata aaaacgataa aagcatcagc
     6181 tcactgtctg ctcgcaacca agtcttaaac aaatcgattg atgcccagaa agaaaaaatc
     6241 accacccttg aaaatgcttt gaagaacgct agtgacagtt ttggtgaaaa cgataagcgt
     6301 actcagaatt ggacagtgca attaaacaat gcaaaagccc aactcaataa tatggagcgt
     6361 gaagttgcta acaacgagaa ggcaattgac gccatgggta ctgaagaagt tgatgctgct
     6421 aagaaaactg atgagcttgg caacgaactc aaggatacag gtgacgaggc tgaaaagtct
     6481 ggaagtaaat ttgaaaagtt cggtggcgtt ctaaaaggta tcggggtagc aatgggtaca
     6541 gtggcacttg ctgctggcgc tgctgccgtg gagctaggaa aacaagtggt atctgcttac
     6601 gcggactatg aacaattagt cggtggggtg gatactcttt tcaaggatgc gtcatccacc
     6661 gttcaaaaga atgccgacaa tgctttcaaa actgccggaa tgtcagccaa tgaatacatg
     6721 gaaactgtca caggtttctc agctgccttg atttcatctc tcggtggtga taccaagaaa
     6781 gccgcaggag ttgcggatca agctgttact gatatgagcg ataatgcaaa caagatgggg
     6841 acggatattg agtccatcca gaacgcctat caaggtttcg ccaagcaaaa ttacgggatg
     6901 ctggacaact tgaaattagg ttttgggggc acgaagtccg aaatggaacg tttgctccaa
     6961 gaggctgaga agatttctgg tgtcaaatat gatatctcga acttcgctga tgtgactgaa
     7021 gcgattcacg ttattcaaac cgaaatgggc atcactggta caaccgccaa ggaagcgagt
     7081 gagacgataa gtggttcgat tgacggtatg aagtctgcaa tctcaaactt gatggctgga
     7141 cttggtaaca gcagcgctga cattggtatg ttgattggca atgtggtgga acaatttcaa
     7201 aacgtcatca agaatattct accggtcatt caaaatatcg tgtcggcttt accggcagtg
     7261 gtggatgcca ttattcaagc attggggcag ttattgccaa cactcttaga agcagtaacc
     7321 tcattattct cacaagtgct tgaaacgata cttggcttac ttcccacact tattccagtg
     7381 gtggttcaag ccgttttaac gattgttcaa acacttattg agaacttacc tttactgatt
     7441 gacgctgcct ttcaacttgt gacaacactt atccaaggac taggcgaagc gctgccagaa
     7501 cttattccaa ttacagtaca ggcaatcata acgattgtga acggcttgat tgaaaacttg
     7561 ccattactgt tggatgctgc tttacaactt atcatgggac ttgcccaagg acttatcaca
     7621 gcacttccaa tactgattga aagtctgcca cagattatta caggaatcgt gaactttatt
     7681 atcagtgcta ttccacaaat cattcagact ggtattcaac ttttgacatc acttgtgaca
     7741 gctttgccgg atatcatcaa tgcgattgtt gcagctattc cagtgattat tcaaaatatc
     7801 atcaatgccg tgattggggc aatccctcag ttgattgacg caggtattca gttacttgtg
     7861 gcattgattg gtgcgttacc gcaaattatc acaacgattg cgaatgccat gccgcaaatt
     7921 atcaacggaa taacaggaac tttggtaaac aacatcgaca aaatcatcaa tgctggtgtg
     7981 aagttgcttg tggcattagt tcaaaactta ccagcaatca tttctgccat tgtcaaagca
     8041 gtaccgcaaa tcatcacagg gcttgcgaat gctttcggtg agtatgtttc aactatggga
     8101 actatcggga agaacttgat tacaggattg tggaatggta tcgctgatgc tggaagttgg
     8161 ttacgcaaca agatttcagg attctttggc ggtgtagtag acaacatcaa ggacttcttt
     8221 ggtattcatt caccatcaac actgtttcgt gatgaaatcg ggaagtacat ggcacaaggg
     8281 attggcgtag gttttgataa ggaaatgact ggtgtttcta aaacgatgca gaaagctatt
     8341 ccaacaaaac tttatatgcc aagctttgat attgataccg atatccacgc tgccgttgat
     8401 agcacattct cgatgcttga tttaggtgct aaggttgatg ccttaacaaa catcacgagt
     8461 gaaatgttgc caacgctact taaagcaatg gacattaaag tcgtcttgaa tgatggaaca
     8521 ttggtcggca aacttgctcc agaaattgat aagagcttgg cgacgttgaa tcgtagacgt
     8581 gtagcatatt agtgttggat tttcacattt tgactgtata ctataactaa cgagtgttta
     8641 ctgaagagat ggtggtaatc atatgttgaa gcataaattt aaagcaaaga aacctgcact
     8701 gataggatat attctactgc ttgctattgg attattttta acggcagtaa gatggtatag
     8761 cggcataaat catggttttg tattcatcaa caatcagatt aattctcaca tttcaaactt
     8821 cgcattaagc tttcttttct atttggtgat aggctatctt tggttgctta tgggaacgaa
     8881 tttaagggtt cttactgcga tgggagctgg tctgattgtt gcaaatattg tatgtgaaac
     8941 gatgatgact tttctgaata caccagatct aatcgatgca atatatggaa tttccggcgt
     9001 gctggttgta tacgcgtatt tagcgattac caagaaaata gggctggaag aaacaaccga
     9061 ataggatgat agattataaa ctttaggatt agacattgtt gtctggtcct tttttagtta
     9121 tttagatggg cggtggtcaa ttgaacgcat ttattttaga cggaacaatt aattcacgga
     9181 ctgatttagg tttgcgaatt acggaagtgc cagttattcc agtgacggaa cgtgtcattg
     9241 atagtattga gattgatgga cgtgaaggag atttgaccat tctgaaaggc tggaaagatg
     9301 tgactttcgg ttttaaggta gtgatttgga aatctgatgt ttggactagc tggcagataa
     9361 ttttgccaca aatcctaaac gctaaaacta tcgcattttc aaatgaacca aacgtgtttt
     9421 acccaatcaa gtatgctaag gcgagcggac taactcagat tttatcttcc atgtgggagt
     9481 ttgaaatgga gttaacctgt tcgccttttc gttataagac caatgtagcg actatcaatc
     9541 gaaccacgtc aggaacggtc aataatcccg gcaacattta ctcattgccg aagattaagg
     9601 tctacggcaa tggaactagg acactcacga taaatggcaa gcctatcgtg ttaaatctga
     9661 cgaatggcaa cctgacacta gattcggatt tgaaagaatg cttttacggg gatgtggcag
     9721 ccaatcaata catgacaggt gactttcctg agtttcgagt cggcagtaat actgtgactc
     9781 ttggtacagg tataaccaaa gtagaaatag aaccgaggtg gcgatactta tgattacgct
     9841 ttacaacaaa acagaatcaa atttcaatca taacggtctc gctgtccttg atggcaacat
     9901 tctgaacccc gtggtgagtg aggaattgaa cggactcttt tcctttgaat tcgattatcc
     9961 aattcatgct ccacacagtg aggaattgtt gccagaaatg attgtgaaag caccagtacc
    10021 agaactgtcc gaccagcttt tcaggattgt tgaacgagat gatgcacttg gtggtttttt
    10081 gcacatagta gctcatcata ttttctatga cctagccaca aactttattg aagatactta
    10141 tatcgtcaac aagaacgggt cgggagcttt gacacaactg cttggtgcaa ctcaattcat
    10201 gcaccgtttt accggtacat caaacatcag tgcggtcaat aatgtccgct tggtgcgact
    10261 aaatcctgta gaggtgttgc ttgatagtga cctagagaat ggttttcaaa gcagttatgg
    10321 tggtgaaatt gtacgcgata acttcacgat ttcaatgtta acgactcgtg gcagtaacaa
    10381 cggcgtacaa atccgagaca agaagaatct gacaggatat aaagctgatg ttgattactc
    10441 tggaattgtt actcgcatta tgccacaagg gtatgatgga ctatttcttc ccgaaaaata
    10501 cgtggacagt ccaaacatca accaatacgt ccagcctaaa atcaaagtca tcaagtacga
    10561 caacgtcaaa gttggtgatg aagaggatga gtttactcaa gccgaggcct atgatgaact
    10621 tcgcagattg gctgggttag aatactcagc taacaaggtc gatttgccaa aagccaccta
    10681 tgatgtggag tttgccccgc ttgagcgaac tgaagaatat aaagattttg cacagcttga
    10741 aacgattaat ttgggtgata ctgtttcagt tattcatgcc gaagatggat tgaacatcac
    10801 agctagaatg gtctcataca aatacgatcc atttctgaaa gcgtatatct cagtaacact
    10861 cggaaacatc acgccaaaat tcacggatgt ggcgaaggac atcaaccggg tggacaacaa
    10921 ggcggagcaa gccaaagacg atgccaatta cgcattgagt tcggcaaatg gtaaaaacac
    10981 caacttctat ggcccgacaa ctccaaataa tccgaaactc ggtgacgttt ggtacaaaga
    11041 aaatggtgac aagtttgaaa tgtgggtgta tgaaatccgt gatggcgtca ctcagtggta
    11101 tccacttatg acagatatga cggcagaaga agtaaagcaa gccgtggaac aagcacaaaa
    11161 ggatgccaat actgccactg aaaaggccaa tcaagcattt aacgatgctg tgaatgcttt
    11221 gaatgcagct gaaagtgtag gtgcaagtgt tgaaacgctt actacatccg ttgaacagaa
    11281 tactagcaag atttcattag tggcgagtgg tatgtcaact cttgaaggac gagtaatcac
    11341 agctgaatca actctgacag tgcaagctgg gcagattgct agtaaggcaa gtcaatcgac
    11401 ggttgataat ctgacagggc gtattacaac tgcagaaagc accatcaccc aaaatgctga
    11461 tagatttgag ctatctcttt ctaaaacaaa taaagcggtc aatgttttag ccggtatgag
    11521 cattgcagat ttgcagtggg cagttggtgg tctgaataca aatgatggaa aagaaaatac
    11581 ctatggtgga tacgttcgct caggttttac aagagttaga aatggcgaaa agtatctact
    11641 tcaaacattt gtgggtggga aactttactc tacttacgca acggcttatc tgctttatta
    11701 caaagaggat aagtcctttt tatcgtacac ccaatttgga aacaccacca caccgcttac
    11761 tgttccaagc agtgccgctt ttattcgcat tcgtttaaca actagtgatg caccagaaac
    11821 catcagttgc tatatgttga aaacggatgt gacaaatggc tatgttgatt tatccgagat
    11881 aagtaacgtc gtaaaactta cagcgaccac tgatgcctta ctgttaagtg tttcagaaac
    11941 caaagaagca atcggcgttc catttaaagt caatcaatgg gaacaaggaa gtttgagtac
    12001 cactgacggt tcagaagtgt ccgcaaccaa ttatctccga agtgagttcc ttgatgtcaa
    12061 agtgggagat aagtatatcg ggcaactgcg tgacggaact caagctgctt tctactatca
    12121 ttactacggc atggatttac cttacattga ttttgtccca acggcaaaac aatttagtga
    12181 aaccctaacg gcgggaactt atactaatgc gaccattgcg agttatctag taagtaagaa
    12241 cgttgaatcg atgagtatta caagtggcgt gacaacaaac ccctattcaa attcaggaga
    12301 ttatctggtc atttacaaat tgccattgga aggcaaagtc gctccgagtg taatttcgtg
    12361 gaatggtcgc aaggatacat cagataatat tgtcttgctt tatacaggtg gtgcttggga
    12421 acagatttcg accgttacaa gtagcagcaa tgccacttat tcttggacgt taacggctga
    12481 gcagatatca gggattacta ctgacagcgt gtatctagct ttcttttcag ttaagactgg
    12541 cacatatgct gggatttata attcaagcaa cacacctttt actctaaacc cgtcggatgg
    12601 ttcaagttat catcaaatct cttatactgg ttcgagcgga gcaattacag tcccgagtgg
    12661 cacattgaaa atgcgagtcc gagtgaacac gagcatcaaa cccgatgaat atgaaggaaa
    12721 tatttatcct acgaccacaa ggcaagacta taccaatgcc aacacattat attcagcaat
    12781 tctcatgcag aaagacttaa tcaatttacg agttggtaaa ggagacgtga ttaatcaaat
    12841 caatatctca cctgaaagta ttttgattgc tggaaacaag gtgcacatca ctggtcaaac
    12901 aaccattgat agtgcagtga ttagttctgc catgatccaa aatgcggcca tcacaaacgc
    12961 aaagattgca gatgccacaa ttcaaagtgc aaaaattgca agcattgatg cggcgaagat
    13021 taccacagga actttgtcgg cagcccgtat tggcgcgaac tcgattacgg ctgataagat
    13081 tgctgctaat atcctaacag cattgactgc gagttcgtcc attcgaatca caggaactac
    13141 gattggatat tattcaggaa gcactcttgt gactgaaata aactcgcagg gaatgaccat
    13201 tcgacgtggt ggaacgacag tcggaacaat tggtgcaaat aacatcagtg gtcactctga
    13261 ttggcgtgga ttggtatttg atcttgagta tggaacagag tatatgtcat gggctcataa
    13321 agattcctcc tctgcgagtg tctacaccca gaagctttct tggtatgcga gaacattgca
    13381 gtctggtatg gagaaggggt tccactttga cgacgacatt tatttaccat caatgcttcg
    13441 tatagggaca caacatcaaa tatctgttgg tgggattaca tttagtagtt ccctttatac
    13501 taagattggc tcaagttccg gcagcgctgg tattgcattc aacggttcta atctgatgct
    13561 cggagatgat gggaagtggg ttgactttgg agtcatccgt gaaatctgtg ccaaacttgc
    13621 gggacgaaca atcgctttac caaccggttt cagtagcaac ggtacagcga gtggttggta
    13681 caatccgcag tcgtttaatt caatgaccac atacagttag gaggccgcca tgaacgaaga
    13741 aaaagaagaa gtgatagtaa ctgaacctat cgaggcagat attccaaagc caatgaaagt
    13801 tgggacggat atggttgatt tgcttgtgat ggtcgtggat gatctttgtg tccagattgg
    13861 tgatttaaag gagaagatag ataatgctca aaattagaaa tgaagatttg gtggacgtga
    13921 tgagtttctt aaaaggtttt gacttaccac caaagattag ccgagtgcga acgaagcttg
    13981 caaaactcat ccaggtgaag gttgatgaac tctatcaaga cgaggtagac ttgcttcaaa
    14041 aatacggaaa gaaagatgag cacggcaatt tggtccaaca tgacggtgcc ttttcactca
    14101 tcccagaaac cgcaactgaa tatcacaaag aaaaggcaga cttgctcaac gaagaaaccc
    14161 acatcaacgt ggatgaactt caagacaagc tgcctttatt ggttgctgga tttgaaaata
    14221 gtgaagtcac catttcaggg aaagatgctg aaatgctcga tttaatttta gaacaattgg
    14281 aggatttgta aatgaaagaa gtgtggaatt ggattcaact cgccatcgca ggtggtggag
    14341 gagtgattgg atggtatgtg ggaggaattg atggtttgat ttatacgctt gtaacttttg
    14401 tggtagtgga ctatgtaact ggtctgcttc gtgcaatcaa cgaaaagaaa ctatccagca
    14461 agattggggc aaagggcatc actaagaaaa tcatgatttt tcttttggtt ggtgtggctc
    14521 atatgcttga tgtggaagtc atcaaaggtg gaaatgtact gcgtgatgcg gtcattttct
    14581 tctatatctc aaacgaagga atttcactgc tggagaatgc cgtggcgatt ggactgccag
    14641 tgccggaaaa gttgcgtgaa atcttgcagc agttacacga aaagaaggag gaaacagaaa
    14701 atgggaatta gtaatctagc aacaaaatat ggatttcagt catttagcca ttactcgaat
    14761 ggtcgtagtg gtgccaaagt agataagata gtgattcatc acatggcaac caccaactat
    14821 gatattgttc ctgatatttg gaaaacgcgt gaggcgtcag cacactacgg tattggaaaa
    14881 aatggagaaa ttcgagcata cgtggacgaa ggaaacacag cttggcacgc tggtaattgg
    14941 aacgccaata tctcaagtat cgggattgag cactgcaata gcactggatc accgaactgg
    15001 ggtatcaatc aagcaactat tgatgcaagt gcaaagttgt gtgcagatat tgccaaacgt
    15061 tatggtctag ggaaattggt ggtgaacaag aatatcttcc ctcactcaca tttttcggcc
    15121 acatcatgtc cgggacagtt gcttggtaaa ctgcaggaga ttgcggataa agccaatgcg
    15181 attaataatg gttcatcaac cccaagcacg ccagctagtg gtctgtatcg tgttcgcaaa
    15241 tcatgggctg acgccaagtc acaaattggt gcctataagg acttgaacaa tgcgaagcgg
    15301 gctgcagata gccattctgg ttataaggtg ttcgaccaga acggtaatca agtttatcca
    15361 tctgggtcat caacatccaa cgtgaaaact cttgtggttc aagtgagtgg tctcaacgtt
    15421 cgcaacaagc caagtttaag tggtgcggtt gttggttcat acaataaagg cgcaacttgg
    15481 acgcttccga agtcagagaa gatgacgatt gcggatggtt gggtatgggc tcgttgcccg
    15541 attggatatt gtgctgtcgg taagaatact ggtaagacgg aatctgacga ttatattttg
    15601 atttcataaa tttaatcgga acaaaattat gtccggattg ttgacttaaa atatcgaacg
    15661 tgttattatt aattcaacca gaacataaat ttgttccgat tgaattatag aaagaggtat
    15721 cgatatggtt acgtttaaag atttgttacc agaaccaaca gaaagccaac aagctttcat
    15781 tttagatcac gggaaaacag aagatgatgg tcagttgaaa tacgctgatg atgctaaaag
    15841 ttacggttgg aacatgcgcc aatatggtaa attgaaagcc ggcgcggttg ttttgaatcg
    15901 tcatccgggg aaaattacta aagaccgtaa gtgggaaatt tacgggggcg gatacgttga
    15961 gagcatttct gaacctgatg aagctggaaa tgttacggca gtaattacac acgcatttac
    16021 aatcgaaccg gcaattaaac aaggtgattc atttattgaa aacttcgatt ggaatacacc
    16081 tagcaaaaag aagcgtaaaa agccgaattc atgggcctac ttctgggatc agtatggaat
    16141 gaatgaaatt acctatgccg attttgttgc attgattgaa aatcgtcatc ttatcccaat
    16201 cgatgataca caatcgttgc cgtctgaact ggatcttaca aacgaggaag ttgaagaaat
    16261 tgagcaagaa agttcaaaag gatttactgt tcttgttgat gaagtgggac caaatcgcac
    16321 gaatgggact cagaaacgaa aattcactgg acgccatact gattgggagc gtgtcaacaa
    16381 ggcaaaacaa aaaacgggtg ctctaggcga agaaatcgta cttgatttcc tcattcagga
    16441 agcagaaaag aacaaaacaa aacttcctga acatgtttct aaaactgaag gagatggtca
    16501 tggctatgat atttgtgcgt ttgaccaatc aggtaatgaa atccatattg aagtgaaagc
    16561 ttcaaaaaca aacttctctg acggatttga aatgagtact aatgaggttg ctgcctccct
    16621 tggagataca ccatataaaa tttactttgt tcacgacctt gatgtggcgt caaaagtttg
    16681 caaaatcaaa atttatgatg gcccaattac tgaagaaaac ttcacgatgg ttccaacgag
    16741 ctacaaaatt ttcaataagt agtttgacgt ctttataatt caaaacagcc tcacaagttt
    16801 tattgtgggg cctttttttc gtctagtttt ctttaaggta ggtgtttaca gcattttgca
    16861 ttaggattaa atgatttctt aactgaaata cctgctcttt gtaattgatg cagaaaagag
    16921 gcgattctat tgattccata cgattattca tcgcgtttat attggcttgt aaaaactggt
    16981 ataaagcttc ttcagcctct atggttttaa agcacttttc aaattcttgt ttgaagcttt
    17041 catcaaattc ctccagttcc tttgttgtcg cagatttgca aaaatgttca tattcttttt
    17101 ctaacccttc catatcaaaa ctactcataa ctttctcctt cccacaattg atatttatat
    17161 tgttatttat tggatgaagc ttgtaaaggc ttatttgatg tttgggaagt aaaaagtcaa
    17221 aatttacaaa gaataattcc tactatatat aaacaaactt agaagcctgc aagagtgatt
    17281 cgtttcattc ttgcaggctt ttttcgtttt gtgggttcga gtcggttatt tctttcgcat
    17341 atggatgaaa ggcaaaggaa aatttgtgat tgggtttact ttcatggatt ttcttcgcct
    17401 gtatcttgag gaggcgaaag caatgactaa caagaaaaca gaaattaact attcagatga
    17461 agcagcctat tacatttcac agcagatcct tctgaactta cttgttaccg gtaaaatcaa
    17521 cctggaagaa tacgtcaaaa tcgacaataa aaaccgggaa acattcaatc cttttttatc
    17581 aaggataatg gtctgaaacg ttgatataac ggcactttag agctaacatg tcacctaatg
    17641 gaaaggagtt gagctgatga aaacgataca taaattggca cgtccagagt tgccggaagc
    17701 ttccaaacta aaggtggcag catacgctcg tgtatcaaca tcaagtaatg aacagctggc
    17761 aagtttacaa acccagatta ctcactatga aaatcacatc aagaaccatg aacaatggga
    17821 ctatgtcggt gtttattatg atgaagggat ttctggaacc aagactgtaa aacgtgatgg
    17881 tttaaaacga ttgattaagg atgcagagct tggaaagatt gatttgattc tgacaaagtc
    17941 tatttccaga ttttcaagaa atacagttga ctgcttgaat ctggttcggc agctaactga
    18001 tattggtgtc accatctttt ttgaaaagga aaacattaat actggtgata tggaaagcga
    18061 gttgttgctt tcaattttat caagcttggc agaaagtgaa tcctattccc attcagaaaa
    18121 cttgaagtgg gcgattcgaa accggatgtc taaagggaaa ttcaaagctt acccgccgta
    18181 tggctacaag cggaaaggct atgattttca catcgttgca gctgaagcaa aagttgttaa
    18241 acaaatgttc cagtgggcat tagatggcat ttcgactgga atgattgcca agcggttaaa
    18301 cgatggaaaa caattcacta aacgtggggc aaattggcaa gcaacatcag tcagaagtat
    18361 tttgaaaaat gaagtttata ccgggacttt gacgatgcaa caaacctttg ttgatgacca
    18421 gtacaaacga cgcataaaca agggcgaatt gccaatgtac cagatcaaaa acaatcacga
    18481 acccatagta tctaaagaag attacgaccg agttcaagaa ctcattcgga gaaaagctga
    18541 ggccaaaggg aatactggtg acgggaaaaa gtacaccaag cgatacgcat tttcaggcaa
    18601 catcatctgt gataaatgcg gcagcaaatt caagcgggtt cacacttatt ggaaaggtaa
    18661 tacgaagaag gttcagtgga agtgtacggg gcatcttaaa aataaagata gctgcgatgc
    18721 attaccaatt acggatgaag atttgaaggt tgcttatttg accatgttga ataagttgat
    18781 tttcggacac aagttggttc tggaaccttt tatgaagtct ttgaataaag caggtgcgaa
    18841 tcaggaaggg ctcgaagtgc ttgctgatga aatcgacaag attcaagaaa agcttgaggt
    18901 tctggcaagt ttgactgctt ccagtatttt atcaacgaag gtgtctttag aagaacaaag
    18961 caaacttcaa caagagaggg ctgtcgcaca agaaaagcag cgcctcatca ttgagtcagt
    19021 caacggcaaa agcactcagc aaattgaact agaagcctta tacaaattcg cgcggatggc
    19081 agagatgttg acggactggg atgaagattt attcaagaag ttcacaaaca gcattacagt
    19141 ttacaaccgc caagaagtcg gatttgaact taagtgcgga ctacttttga aagagaggtt
    19201 accagaatga acaatacaat tccatacggg tatgtcatta agaatggaga aatcaacgtg
    19261 gatgaacagc aagctcaaca aatacgtgag ttcttcaata tttaccttga gggtgttggc
    19321 ttacaagaag ctggcaaacg ctctggcatt aacaagccgc atccctttat gaaacgtatt
    19381 ttgatgaatg aggtttactt aggaaatgag atatatccaa aaatcattga tgaggatatt
    19441 ttcttccaag cggatctgga gcaagaacga agaattaagt tctttaaaaa acgcaaggag
    19501 atcaaagtgc cagatacgaa ggtaataccc actcatttta aatctggcaa gattaccgaa
    19561 aagtattctg acccattcaa acaagcgcaa tatgcatatt caaaaatcaa ggaggttaag
    19621 tgaaatggca ggaaaacagg tgacggttat tccaatgaag cctagacaat gggcagcaga
    19681 gaataccgaa gagaaaccaa agttgaaagt ggcagcctat tgtcgtgttt ctactgaaat
    19741 ggaggaacag gcgagttctt acgaagcaca ggttcaacac tatacggact acattcaaag
    19801 aaatcccgat tgggagcttg taggaatatt tgcggatgaa gggatttcag gaactgggac
    19861 gaaaaagcgt gacggtttta accggatgat tgaggcgtgt caaaacggcg aagttgagta
    19921 catcatcaca aaatcaattt cacgctttgc ccgtaatacg gttgattgct tgcaatacat
    19981 acgacagtta aaagaactga agattgcagt gttttttgaa aaggaaaaca tcaatacaat
    20041 ggatgcgaag ggtgaggtac ttttgaccat tatggcaagt ctggcccagc aagaaagcca
    20101 gtcactaagt caaaacacca agatgggcgt acagtatcgt ttccagcagg gacagctacg
    20161 tatcaaccac aatcattttc ttgggtacac taaggacgaa gatggtaact tggtgattga
    20221 accaaaggaa gcagaagtca tcaaacgcat cttccgcgaa taccttgagg gcagttcctt
    20281 gcaagaaatt gctaatggtc taatggctga tggcatccta acgggaggga agcgaaagct
    20341 ctggcgagga gaaggagtca gattgattct tcgaaacgag aagtacatgg gggatgcgct
    20401 gcttcaaaaa acatatacta ccgatttctt gacaaagaaa cgagtgaaga atgacgggag
    20461 ttacgctcag cagtattatg ttgaagatag ccatccggcc attatcccaa aagatatctt
    20521 catgcaagcc cagcaagaac tagaccggcg taaatcaatg aaaaacaaaa agtcacagtg
    20581 cttttctggg aagtatgcgt tatctggtat cacagtttgc ggtgactgtg gcaatgctta
    20641 ccgcagagtt cattggaaga atcgaggaac tgtgtggcga tgcaaatcca gagtggataa
    20701 acgagaacat aattgcacgg gtcgaacaat ctacgaaaca gacctgcatg gagctgttat
    20761 caaggcaatc aatgaaacgt taatcgatcg tgatgctttt cttcagcaat taaatgacaa
    20821 tatcaactca gttatgactg atggattaac tgaccgattg gcagaactag acagtcagtt
    20881 gaaagagctt gaagctgaaa ttattagcct ggcagttggc ggtcagggtt acgatgaatt
    20941 ggcaagccaa atattttcac taagagatga acgagatgcg gttgcaaaac aaatagcagc
    21001 gaatactaat ttgcaacaac gggttgatga gatggtagtc tttgtaaaag aacacgatgt
    21061 catcaatgag tataatgaag tgcttgttcg aaggttgatt gagaaggtca ccatttttga
    21121 aaagaacatt gttgttgatt tcaaaagtgg ggtgagagtg actgtagaaa tttaatttga
    21181 atagatagga ctagacgtta atgtgggtag ccatattaac gtctttattg attggatttc
    21241 ataaagtgat tgatgatata atatattatg taaaaaatag gctgggggcg aagttctgtg
    21301 agccaatgga ataccattga tgaattaagg gatattttta ttcgtaaata tacggattct
    21361 gagttgagta atgaacttga aaaggcctgc agcgaagaat atgaattgat gcgtgattat
    21421 aatgggcgtc agattttaga gttattacag aatgtagacg atgcctatgg agatataaaa
    21481 tcagaaaatg atgctagcaa tgacgaagtg aaagttcaga taacatataa aaataatatc
    21541 ttggaagttg ggaatacagg cacttcattt tcaaaagata caattgaaag attgtgctta
    21601 ggaagagcga gtaataaatc atcacaaaat atcggaaata aggggactgg ttttcgttcc
    21661 ttgctaaacg atgctgagtg ggtcgaactg tattcgggag aatttgctat tcgatttagt
    21721 gaagaattca caaaaaagct ttttgagcag tatgtatcaa gagattctga caaattcagt
    21781 gaattaattt ttgagcagaa gaaaaattgg aaaaaagaag attatgattt atgttttcca
    21841 ataatgaact gtcctcaacc aataaacaaa attgattctg gttttgatac attaattcga
    21901 gtaaaactaa aagaaactaa tttaagtaaa ggcacgagtg tttctaaaca acttcagcag
    21961 ccattttata agtcactgtt atttttacca aatataacaa aaattgttat tgaaacaact
    22021 gatgatgaaa aaatatcaga gaaaattgtg gatggaaatg atgtattgat agaaaaaagt
    22081 gatgttactg gtcaatcacc gtctgaagaa tattttgttt ttgacaaaga atctatgata
    22141 ggagataaag ttgctaaaat tgcactagcc gtttctaaag attctgatta cgattactcc
    22201 aaagaaaaac tatattgcta ctttccagta cgtaattttg gtacaccaat tcatgcactt
    22261 ataaatgcac cgtttataac aaataattca cgtgatgacg tcccggatga tagtgaacaa
    22321 ataaataaga aaatatttat tgaggtgctc aaatttattt ctgaagtagc agaaaaatta
    22381 tctaagcctg aatatgaaga catagcatta aaaatggtaa ctccttttaa agagaataaa
    22441 ctatgggact tagatgtatt tgacttaatg aacgaattct tgggtatttt atctaatgcc
    22501 caaatactac cgactgtcaa ttggcaatat atttctatca aagataatcc aaaacttttc
    22561 cttcaagaat tccctgagga gctaataggt gagacatttt ccaccttgtt gcaattatta
    22621 gaaacaaacg aaaatgacca actagtacaa gaacttgcta attataatga atacactggc
    22681 ctgtcgtttg ttgtagatga actatctgat aaaatcaaca ttctttcatc ttcttgggaa
    22741 aatagaataa ggacaaaagt gttcttatgg tggagtgagc aatataaaag ttctccgatt
    22801 gtccctaagc tattaaagga caatagtgac aattggttga tgaaatcaga taaggtattc
    22861 ttaccgaccg atacaggtgt tactgtgctt ccaaaagagc tgtcttcttg ggttagttta
    22921 agcattttga aacaaacata tgttgatgag ttaatttatc agattaagac ttcatcaagt
    22981 tggaaaaata aatgggaaga aacagctaat gcatacaaag ctgaacgtac aggagataaa
    23041 cgtattctag atgcattcag tgaaaaatat cttgcaattg aattcactga gcaaagcagt
    23101 tcagatttga tcattggaac gattaatcga caaattgaca ctgttgaaaa atctatcagt
    23161 ttcattaatt ggttttatga taactacaat gaaaaactat cagctggaag tgagctgtct
    23221 aaagttccat ttaatcttcc aaacgttgct ggtgacgttc aaccttccaa taaaatgtat
    23281 attggaaaag agtatggcaa tgaattagga gataagcttt ttcaagatac agattacacg
    23341 gcattgaaaa aaatagattg ctctagtttt gctgaggaga gagattttct gtcatttatt
    23401 cttcgatgtg gtgtttccaa atatcctaag atttatgata acgattcttt aaaaaatgat
    23461 gttggat
//
LOCUS       DAZUKA010000029        23444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000029 DAZUKA010000000
VERSION     DAZUKA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.029"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..349
                     /locus_tag="ACG8PN_000918"
     CDS             32..349
                     /locus_tag="ACG8PN_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4861093.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            342..950
                     /locus_tag="ACG8PN_000919"
     CDS             342..950
                     /locus_tag="ACG8PN_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4861094.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1005..1796
                     /locus_tag="ACG8PN_000920"
     CDS             1005..1796
                     /locus_tag="ACG8PN_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4861095.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1934..2422
                     /locus_tag="ACG8PN_000921"
     CDS             1934..2422
                     /locus_tag="ACG8PN_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4861096.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2473..3345
                     /locus_tag="ACG8PN_000922"
     CDS             2473..3345
                     /locus_tag="ACG8PN_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4861097.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3521..3883
                     /locus_tag="ACG8PN_000923"
     CDS             3521..3883
                     /locus_tag="ACG8PN_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4861098.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3892..4644
                     /locus_tag="ACG8PN_000924"
     CDS             3892..4644
                     /locus_tag="ACG8PN_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861099.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4658..6460
                     /locus_tag="ACG8PN_000925"
     CDS             4658..6460
                     /locus_tag="ACG8PN_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861100.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6737..7777
                     /locus_tag="ACG8PN_000926"
     CDS             6737..7777
                     /locus_tag="ACG8PN_000926"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4861101.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7829..9973)
                     /locus_tag="ACG8PN_000927"
     CDS             complement(7829..9973)
                     /locus_tag="ACG8PN_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4861102.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(10330..11703)
                     /locus_tag="ACG8PN_000928"
     CDS             complement(10330..11703)
                     /locus_tag="ACG8PN_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4861103.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11703..12098)
                     /gene="rbsD"
                     /locus_tag="ACG8PN_000929"
     CDS             complement(11703..12098)
                     /gene="rbsD"
                     /locus_tag="ACG8PN_000929"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4861104.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12126..13043)
                     /gene="rbsK"
                     /locus_tag="ACG8PN_000930"
     CDS             complement(12126..13043)
                     /gene="rbsK"
                     /locus_tag="ACG8PN_000930"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4861105.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13151..14164)
                     /locus_tag="ACG8PN_000931"
     CDS             complement(13151..14164)
                     /locus_tag="ACG8PN_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4861106.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14245..14844)
                     /locus_tag="ACG8PN_000932"
     CDS             complement(14245..14844)
                     /locus_tag="ACG8PN_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4861107.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15030..15731
                     /locus_tag="ACG8PN_000933"
     CDS             15030..15731
                     /locus_tag="ACG8PN_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861108.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15741..18452
                     /locus_tag="ACG8PN_000934"
     CDS             15741..18452
                     /locus_tag="ACG8PN_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4861109.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            18903..20531
                     /gene="treC"
                     /locus_tag="ACG8PN_000935"
     CDS             18903..20531
                     /gene="treC"
                     /locus_tag="ACG8PN_000935"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4861110.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(20591..21772)
                     /locus_tag="ACG8PN_000936"
     CDS             complement(20591..21772)
                     /locus_tag="ACG8PN_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4861111.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            22024..>23444
                     /locus_tag="ACG8PN_000937"
     CDS             22024..>23444
                     /locus_tag="ACG8PN_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFS4861112.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT         7625 a         4195 c         4784 g         6840 t
ORIGIN      
        1 aatattacac ttgagaatag aaggtgacgt aatgcacatc cagattccaa ctgttcttct
       61 tgatggtact gtgctagcag cattaagcaa ggaagatatg tatgggtatg ccttaacaaa
      121 aatcgtacaa gaaagtctct ctgtcagtga atctacacta tatccggttt tgagaagatt
      181 gaaaaaagaa ggatatcttg agacctatga tgaacctttt gaaggcagaa accgtcgata
      241 ttactcgttg acaccaatgg gaagtcgtca tttagaagaa attattgatg aatggattgt
      301 ttttcgagat tctttagacc ggttattgga ggagagcaca aatgaatgaa tatttgaatg
      361 aagttgtgga tcatcttcaa gaaattcctg aagacgatcg ttttgatttg atccagtatt
      421 atgaggaata ttttttagat tctggaaaaa cattagatca gatcattgat gaatatggta
      481 caccaaagca gtttgcctta aaattaaaaa tcagttactt ttcagaagaa gaacaagcag
      541 aaaacgagtt agtgaaaaat ccaaaaagac aaattcgcct gatttggtta atcattatcg
      601 gtttatttgc ttctcctgtc ctcattccac tagcactggg gtttatattg gttattgggg
      661 gactattggt tgctttacta gcgctcattt ttagtatcta tgttttatgt atcagtatct
      721 taggtgttgg cctattcatg atcatctcag gaatcagtgt gtttggacaa tcgattctga
      781 gtggattatt ctttatcggt gtcggtctgt tcgccacagg aagcgcagta tttatttcgc
      841 cactgttgct gaaaggaaca aaagggcttt tttatgtcat gatgaacttt gttaaatgga
      901 tgggacgacg ttttattaca aaaagagatc tgcattcagc gggcgtttga aggaggacac
      961 ataaattgag attgaaacat tttttgatta ccggtattgg tctgatgctt actggtggcc
     1021 tcctagcagg tgcaacttat acattaggcg ctcaaaagag tctgacttgg gataatggtc
     1081 ctcggttgat cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca
     1141 tccatgcaga aaatcaaaca gtttcggtca tacgcggatt ggattttgag ataaaaacga
     1201 cttatgataa gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg
     1261 cagtcaaaaa atcaacaggt gcaatcatta gtttagaaga cacaagtgca caaatcacta
     1321 ttgttgtccc tgcagatcat gaattatctg aattgacaat cgaagggaat aatagtgcaa
     1381 ttgatatcaa cgaagtatca atcaaaaaag tgaatactaa gacacatgat ggctgggtat
     1441 cgttcaatga atttacagga ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat
     1501 tagatgaggc caatgtggat aacttgatcg tcacaggtga aaacagcaac ttccatctag
     1561 aagacagttt gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc
     1621 atgagatgga aaacagtggc tatactatcc aaacgtctgg tgaatcttat attgaaaaag
     1681 actaccagcc aataacaaat actttgttag aacaaggaca gaaaaagcta aagatcactg
     1741 ctaaagacac atcgatcaat attacgacgg atcaagagga caatgaaaca gattgatcct
     1801 gtactcaact ggatatttta agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt
     1861 catcgcttaa cgcacataac tatagtaaaa taagaaagaa aatatctaaa aagaaaacag
     1921 ggtgaaagag tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga
     1981 aagattacat acttatatca ctcaactaag ccaaaccttc tatgatccag acaaagaaaa
     2041 agtgatggtt aattacccga acaattcaga aggtcgccaa ttagagcaag tgtaccatga
     2101 agttttcaaa catttattga cggtgaagaa agagctggat tattattctt taccgattat
     2161 cgatacagga atattgaagt atgatgggaa gaaggaacga tttatcttta aatctgttcg
     2221 ggaagatttg cctctttcag cagggatgga tctagaggtc ttggtagaag actattttac
     2281 agaagaaaag cactgggttc gaacaaaatt ggattattta ccgcaggcag ccggagggac
     2341 acaggcaagc ggttggtaca tcactgaaga caaagagtta gaactagaag gtgtaatggc
     2401 gcgtattcga aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg ataaaggagc
     2461 gcaggaagta gcatgagaga aaaaagagca ttttatatca atggttatgc agggattgtc
     2521 ggattggtcg tattagcttt agttggattg tttttcttct atttaggtat gtggcaagcg
     2581 aaagtttggg cacttttttt aagtattttt ctttggttga tcacgttatt gttgttaagt
     2641 tcggcaactg tagtcagtcc taatcaagca aaagtaattt tgttttttgg acaatattta
     2701 ggtacgattc gagaaaatgg ctttttcctt acgatacctt tggcacaaaa aatgactgtt
     2761 tcgttgaaag ttcggaattt caatagttct gttttaaaag tcaatgattt ggatggaaac
     2821 cccatcgaaa tctcagcagt agtagtcttt aaagtcgttg atacagcaaa ggcattattc
     2881 gatgtagcgt attatcaaga ttttgtagaa atccaaagtg agacagccat tcggcatatc
     2941 gctagccaat acccgtatga tacattcaat gagaatgatt tgacgcttcg aggaaacacg
     3001 acggcagtgt ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc
     3061 gaagtaatcg aaacacgctt gaaccattta gcctatgcaa cagaaatcgc tagtgccatg
     3121 ctccaacgtc aacaagcaaa agccattttg tctgctcgac aaaccattgt tgaaggagct
     3181 gtcacaatca ctcaaatggc cttagagcaa atcgaagatg gacaagaaat caatttcacc
     3241 gatgatcgga aagtccaatt gatcaataat ttacttgtct caatcattac agacaaagga
     3301 acgcaacctg ttatcaatac aggcgacatg aaagaccgta agtgacatac tttttgtaag
     3361 ataggaagaa ggagtttagg acggattatt gtcttgaact ctttttttag ttgttttaaa
     3421 gaaaatgaca aaattgaaag aagaatgata gttgattcga aggcagaatt gttcgctttt
     3481 ttctataatc agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg
     3541 tctacttgtt ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt acgtagttat
     3601 gggtggagaa gaatactatg tacaaattac gacagatggt acaaaaacag tttcaaaatc
     3661 agactctgga caagaaacag tcaactatag atatatcctg cctggctata cgaaagaggg
     3721 taaggaaaaa gaactggact tcaagggaca attgactcga ccgcttcgta aaggagcata
     3781 tttaaaaatc acctggaaca aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa
     3841 agacgtgccg aaaacagcac tggaaaaact aattaaaggg tagggaaaga aatgaacaaa
     3901 atagtcgaag tacagaatgt aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa
     3961 gcattaagcg gcatcagctt cgatgtagaa aaaggcgaat ttattgggat catgggcgct
     4021 tctggttctg gtaagtcgac acttttgaat attttatcca cattagataa accaacagat
     4081 gggcatatcc gaatcaatca aaaagatgtg accacattaa aaggaaatca actagcagat
     4141 tttcgtgcga atgagattgg ctttattttc caagatttca atttattaga aagcttgaca
     4201 gcacaagaaa atatcgctgt tcctctttct ttacaaggcg tcagaccaaa agaaatcaag
     4261 caacgtgtcc aaaaaattgc agaacgttta tccatctctc atattttgga aagctatcct
     4321 tctgagattt ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa
     4381 ccaacgattc tattagggga tgagcctaca ggtgcgctgg attccaaaag tgcccgggat
     4441 cttttagata cgatggacga attgaataca aaagatcatg tatcgattct tcttgtgacg
     4501 catgatccgt tttctgcaag ttattgccaa cgtattttat tcatcaagga tggcggtatc
     4561 catcaagaag tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga aatactatct
     4621 attcttggaa acttggaaca gtagggggag attgactatg ctttggaaat tatcacttac
     4681 tgggatcaag agccgtttaa gagactactt ggtgttattc tcaggattag ttatggcatc
     4741 tgctattttt tacatgtttg aatcaatggc aagcaacgaa gcttttttga aaagtaatac
     4801 gatcgttgcg gctacagtta tcattttccg attcggttct gttttactag ggatcatcac
     4861 attcgtttat attttatatg caaattcctt tttgatgacg atgcggcaaa aagactatgc
     4921 aatgtttatg atgctgggag caaaaggcag aaaaatcgca cagatgattt tttctgaaac
     4981 attcttagta gggatcgcag ctactttagt gggttcagct gtcggtgtag gattgacaag
     5041 tatcgtccac cgattattag tggatcagtt agatctcaat atttcgcatt ttacaccttt
     5101 tagtattcac gggttgttga tcactgtttt gttttttgct gttttgtttt tattagctgc
     5161 tgttgtcaat gctttttcga ttgtcaaaaa accgatttta gcattgattc gtgcggatca
     5221 gacaccaaca agaatcaagc agcataaatt cttgtttttc cttgaagtag tactgggaat
     5281 tgcttttcta ggctgcggtt actatatgat gaaacacgtg gggacctatc aagtctttgg
     5341 tataggggta gccttagtta cgatcgtatt aggaacttat ttcattttcc attctgtgat
     5401 cattttcttt ttatcattac taaagaaatc agaacagatt tcattgaaaa aattgaacaa
     5461 tttcacattg tcacagttaa actttcgtat tcgtgaatat acacaaatgc tctcaatggt
     5521 cgctatgctg tttgccttgg cactgggcgc tttgacggta ggattaggtt ttaaaaatga
     5581 aattgcagac ttgacagaaa acaattctgg atatgatcta gtgatgaata atcctcgggc
     5641 agaagatcag caaaaaatca aagaactttc accaacactt tctgcttctt atactcaaaa
     5701 agaagatgcg ggaacgattt actataatgc aagtgaattt gcggctcaac cattgatcat
     5761 gataaaacat gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa
     5821 aatgaatgaa ctgactgtac aagaggaact tcgaagctat gaattgccgg aacagcaaga
     5881 aaaagaaatc aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc cacaatccac
     5941 tttacagcta gttcaggtaa aagacttcca agcaaacctg aaaccaatcg aaacacttgt
     6001 tgctcaaaat aaaatgaaca atccatcttt gcaaaaagta gagtacagca accagaaata
     6061 tgaaatgtat gaagtgtata atggtctatt ctctggtttt gaatttatgg gattcttcct
     6121 aggtttagct ttcttgacca tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag
     6181 caagagtgac attgctcgtt atgaaatgct tgaaaagata ggaacacgac gcgggttgct
     6241 taaacaatcg atccgtagag aagtaggcgt gttattctta gcaccaggaa tcttaggtgt
     6301 agtccatgtc ttattcggat tacaaatgtt tggtctattc atgcaaaatc catacaaaga
     6361 tatttgggtt ccgtttacga tattcttcgt attgtatttc atctactatg tactgacgac
     6421 atggttatat actggaatcg tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag
     6481 taataagaac agccaagcaa aaatagctcc tcatattttt cgtttggttg ggcttattac
     6541 cgcagatgca agcttttgaa caccgtttgt tcggaaaaag aggttgtgac aatatttttg
     6601 tcacaacctt tttttcacgg tcttttatta gaatatagta aattttttct cgttactcac
     6661 ggaataagct gttaagaaaa cggtttccat gtatactgga atcagtgcaa atgaaagaaa
     6721 agagggtgca aggaacatgg aaatcaatga acaaaatttt ggcgaaaact atcacttgat
     6781 tacgatcacg aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg cacgggttgt
     6841 tcattttcaa acaataatcg ataatgaaaa acgagagttg atcttaggtt ttgatgaagc
     6901 aaaagaatat ctagaaaaag acccttatat cggtgcttca atcggacgta tggctggacg
     6961 gattacgaat ggcacattca aattagatga tgaaacgttc gaagtaaaaa cagacccgga
     7021 cactggtcat tgtttacatg gtggtgcacc tagctttgaa gccaagcagt ggcattatac
     7081 agtcatcaat ggtgaaaacg aagcatcggt cattttctat cagactagcc cagatatgga
     7141 aaacggattt cctggaactt tagatgtaga agtacggtat acgctgacga acgatgatac
     7201 atggcgagtg acggtccaag ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca
     7261 tgtctatttc aatttaaccg gtgatccagc acagtcaatc gacgaacata tactttggtt
     7321 gaatagccga ttatttgcta cgctcaataa agatacaacg cctacaggag aagtagtttc
     7381 agtaagtggc acagcatttg acttccaacg tcctaaaaaa ttaggtgagg tctttgccag
     7441 tgattttcgc caatcaatga tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca
     7501 atctggttta ctaaatgctg cggaattgac aagcccagat gaaaaagtat cgatccaagt
     7561 aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat tttggagaag atggaccgga
     7621 aatgcgcggg aataaattag taaatcatgg cggtatcaca tttgagactc aagaactgcc
     7681 aggagcggag cgacttgaaa gttttggaga tgtcactctg gaacctaacc aagtacgcga
     7741 gtttgtaacg gaatacaaaa taaatataaa gaaatagaat aaattaaaaa gggagtgtga
     7801 cagtgtcaca tccccttttt aatttattct atttctttaa aggcagatca agccagaatg
     7861 atttgtctaa ccttaacaag gttagtacag gaacagttgc agctttaact gtacgagtag
     7921 ttgtttctgg tatctgtctt gccgtaaaat tgttccacaa atcccagatt gaagcgacac
     7981 ggaagatgga agaagcgttg ttgacgcgtt ttatcgttga aaattctaat cctgtgattg
     8041 gcacgccatc tgttgtccat ttgattggca catcgaatcg cttagacata ttgaggattt
     8101 cagcgtttgc ttcttcagtt gttgctgctt cttttttcgg ttcatcatat tttgtcaggt
     8161 cgtacgcttc gatgatcgcg ttcaattttt gtgaatattg gcgatctgta gcataacgtc
     8221 cttccaaata tttggttgct tctttgtagt cttttgtttc cgacttccaa gttggtttgt
     8281 agaaatcagg attgccagtc agtccatttt tgatcaagtc ggcatagtct tccaaacttt
     8341 ccttgtaaga aggatattta cggaattgtg cgcggatttg gtagctttgt ccggaactat
     8401 cttgttcaag tgtattgaag gtcacgctgc ttcctttata tgatcctttg ataccaaaaa
     8461 ggttatgatt tggttcgctt gataataagc tgtttccaga tccactttca aggatagctt
     8521 gagccatcat gactgatgcg tagattcctt ctttttgccc gatttcctgt gcatcatctg
     8581 caatcttttt caagaactga tcagttgttt ggttttgact gaaatggaac gagccatcta
     8641 atccatctaa aatagatgag ccgcctgtca gcgaacctga agcagcaggt aaacctgcta
     8701 agtttactcg gacagcaaaa tcaggcgtga aatctgaaat agaagttact cgaactttat
     8761 ctcctggttg aggcgcatgg ataaattgtc cgttcccaat atagatacct acatggtgag
     8821 taacaccgga agaaggtccc cagaagacga ggtcgccagg ttgggcctga cttactggaa
     8881 tctgcgtacc tgcatattgc tgttcgcctg tccagccacc gatataatga ccggttgctt
     8941 tcatatatac gtaataagtc aaaccagaac agtcaaaagt atttggacct tttgctcccc
     9001 atacatatgg ttttccaaga tgcttgaatg cttctcgaac gattgcttgt tggacattcg
     9061 ttgtatttgt aggcaataca gcatcaattg gtgattttac aacaaaatca gaagaagcag
     9121 aagatggatt ctctataccg ctttgatttg ctgtatttgg acgagatgaa gcattgttcg
     9181 ttccgccatt cgatgaacca ttgtttggat ggtttggttc tctctgagta tttccattgt
     9241 tattcgatcc agatgggttt gtttgattcg aagaattccc gctgttcgaa ttattgttat
     9301 tattgtttga attatttgaa ccattattat tgttgttatt acccacatct ggtttgtttg
     9361 gcgtttccgg atctggtgta ctagggactt cttcactagg atcagttggt ttctctgttg
     9421 aagtatccgg tccagcttta aattctgcag taatagataa attgttcgta cctactgtaa
     9481 aggtctttgc atcataattt acttctttgg caccattaac agcgactgaa gcaatttgtt
     9541 caccttcagc tggcgtaata gtgtaagtta ccttcgtttc cgctaataaa ccttctaccg
     9601 ttccactttc cgtaatatcc aaaggttgct gttcagggga atccgctgtc gaagcagtca
     9661 gttggatctt accaatcaaa gaaggagcaa ttgtaaggga atatgtctcg gctggctttt
     9721 cattttctga tggatcagcc ggatcaattt cagttggctc ggtcgatcct gttgttgagt
     9781 tagttgttcc agttgggtct tctgtaaaag aatccgttgt ttcactagta gctgagctgg
     9841 atgtagtcgt atctgttgtg actgtttgat cagctatttg attatctacg gcaaatacgg
     9901 tattggtcga caaaacagac ggagcaatca gccaagtagc agctagcaaa cgtatcgttt
     9961 ttttattttt cataataaac tagtcctttt cctttcattg attttctgtc ttattcagca
    10021 ttcattagac attttttgga acagataatc gtgtcgaaaa gctttttctt ttcataaatc
    10081 tgtctgtgta ttctaaataa aacaatttgt aaataagcaa cgagcattca ttcgtttttc
    10141 tttgacgttc attttattgc atagaccacc tttgtttggg gataaacaag tggggataaa
    10201 aatggttatg catatgtaaa ttataccaaa gtttggaaag tatcacaaaa atttataaaa
    10261 ataatgatcc taaaaagaaa aagactgaaa tatgtgataa acatctcata cttcagtctt
    10321 tttcattaac tattcataag acattaggtc ttcatcaaac ccgctaacga gagtagtgat
    10381 cattgctacg actagactaa cagccagtcc tgctaagtac cagaaaaaag taccggaaat
    10441 gaatgcaggc aatgtggtca gactaccaaa tacaaaagcg atcgctttca cttgggcaaa
    10501 tcccattaca gctccaccag cagctgcacc aatgcaagca gcaacaaatg gttttttcaa
    10561 tgggatcgca attccgtaaa cagttggttc ggtcactccc gcaagtaaaa tcgaagcaaa
    10621 agcagaaccg gagatcgaac gggtttttga acgtttactt cgcagaaata cacctaaagc
    10681 tgcaccagcc attccaaaat tggctgctcc tgtcaacgga acgattcggt cgaacccata
    10741 agcagagata ttactgatca tgacagggtt tacagcccat tggataccaa atattaccaa
    10801 aatattccaa ccaccagcga tgattgcgcc actgatcggt ccgttaatat ccatcatcca
    10861 agtaattgcg gcagctaaac cttcgccaag gtaaacaccc gcaggcccga ataccattaa
    10921 cgtcaaagga accgtgataa ctaatgaaag taaaggtaca aatactaatt gcatattttc
    10981 gcgaatatat tttttgagga atttttcaac aaaagaataa aagaaaatac ctaaaatcgc
    11041 cggcatgaca gtagacgtat actgcatcag tactacagga atccccagaa aatcagtagc
    11101 tgctttgcca ctgtctgaaa gtaaagaggt cagattcggt tcaatcagtg ctgcagcaat
    11161 gacagccgca atatatccat tcactttaaa cgttttggca gcagaaaaag caagaaagat
    11221 tggcagaaaa taaaagacgg cattcgacgc agcagacaaa atatgataag ttccgcttgt
    11281 ttcactaagc cagccaatcg tagtcgctaa aacaaccaga ccttttaata taccagaacc
    11341 ggcaaacagc ggaatgatcg gcgtaaagat ttccgatatc aaaccaaata gtttttcgat
    11401 cggatttagc ttttctttcg gagctgaatg atcgatttga cgcgtcaatt ccttgtaagc
    11461 aggagccaat ccctgatcca tgattatttc tgcatatcta tcgagtaaaa tgaaatactt
    11521 aatggaaggg atttgctgtt ttaatttttc tgcattggct actggctgtt ttttcaattt
    11581 gatacgaagc cgagtcgcac atggaaggac agaatcaata ttgtcttttc ctcccaactg
    11641 ttcaatcagc atatgaatct gctcggacag taccatctgt tcaggattca ttgtcgtttc
    11701 cattagaaga ccactccact cactaatata atattggcgt aaggtgtcat ttcgcctgta
    11761 cgaataaatg catgacagtt attcaactct tttttcattt cttcatgtgg gataaagcta
    11821 atcggtgtat ctggtaacaa taatttgatt ttttccaata tttccgggtt cattgtcttt
    11881 atttcttccg ccaattcaat cgattgtacc tcaagttctt ctaaaacgtt ttctaaaaca
    11941 gataaaaaac ttggaagacc gtttgagaca gccaaatcta ttttttcagt accaaagggg
    12001 acaggcatgc ctgcatcccc gatacttaat ttatcaaagt gccccatttg tgcgattact
    12061 cgtgaaatat ctgaattgat tacttttcct tttttcatgt ttgtttcttc ctctcattgt
    12121 taattttaga ctgctgattg ttctaaagct tcgcggtaag ggatagaagg ttgcgcacca
    12181 taagactgca cagcaacaga agaggctaga tttccataaa ggatcgcttc tttcaagtta
    12241 ctaaggtctg gctgaagtgt gctgcttaat gcgccaataa atgtatcacc tgcggctgtt
    12301 gtgtcaacgg catccacttt aaaagcaggg acgataccgt gttctttttc tgtatgatag
    12361 aaagcgccgg cactgcctag tgtaatgatt actgttccaa tacctaattc atggagtttt
    12421 tcggctgctg cgaccaaact gttatgatct gtcactctga ttcctgtaat gatttctgtt
    12481 tctgtttcat ttggtataat gatatctgtc ttttcaagta gttcagtagg aatcgtttca
    12541 cgagctggtg caggattcag gatcgttgtt tttcctgcgt cttttgcaat agaaaaagct
    12601 aggatcgtcg catctaagct actttcaaac tgagcaataa taaagtcact gtttgtaatc
    12661 agttgcttgt tttttaggac ttcttgttca tggaaagcat tatttgcccc tgcatggatc
    12721 aagatactat tttctcctgc tgcgtctacc ataatcattg cttgtccggt tgtggcgcct
    12781 tggatttcag ctatcccaga tgtatcgatg ttttcttttg tcagtagatc taataactgt
    12841 tgtccttcgc tgtcagcccc aacgcctcca ataaaagatg tccgtgcgcc tgaacgtttg
    12901 gctgccactg cttggttggc acctttacct ccgccagcgt gaaaaatctc atgcgcatgc
    12961 atcgtttcgc ctggcttggg catgttggtt aaacgaaggg tagtgtccat attaatactt
    13021 ccaattactg taaccgtgtt cattttacaa acctccaact atctcattta ttttgatggg
    13081 aaaattattt tttaaaacgt tttagtaaaa cgttttaata gttcgataaa aatatagcac
    13141 accttgaaag ctatttcaag ggtgcagttg aaaatctttt ttctaatcgt acaggtaatc
    13201 ttttttcttc ccattctttt tctgggaatt ggatccgatc taacaataac ttggcagatg
    13261 tttgtcccaa ttcgaagatt ggttgtgcga tcgttgtcag tttcggcttg atatactcgc
    13321 acatatctat attatcatag ccaataatgc tgtagtcttc agggatgctt tttccagctt
    13381 cctctaatcc gcgatataat ccaaaagcta attcatcatt taaagcaaac acaccagttg
    13441 cagcagaatc aagaagttct gctgtgatct ggtagccacc ttgttttgag aactgtgtag
    13501 gtaagaggat cagttggtcg tgagggattt ggtaaacatc aagagcagat ttaaacccct
    13561 caatccgagc gtggacattt tccggtggat tttcaggata gacaagggcg atcgtttgat
    13621 gtcctagact aagcaagtgc atgcccgcta ggtaaccacc gcgaaaatcg tctgttcgta
    13681 cggcatcact aaatccttca gattttttct gatccaaaac aatgaagggc cggccttgtt
    13741 ttttcaaatt ttcgttgatt gcatctgtag agacagcaga agtcgcgata atgaatccat
    13801 ccacacctcg acgtgtgagc tcggctagat attcgatttc tttttgatga tcagaatcag
    13861 cattacataa tatcgtgacg aaattctgct tatacaagat atcctcaatt cctcgcatca
    13921 gtgtactgaa gaatggattc gtgatatcag gaacgagtac accaattgtt ttcgtttcct
    13981 tggtaatcat ttgtctagca aaatagtcag gctgatagcc taattcttct ttggcagcta
    14041 aaactttctg aacagttttc ggactgaatt tctccgtatt tccatttaat atcaaggaga
    14101 cagttgtgat tgaaacgccg gaaagtttag cgacttcttt gattgtgatc ttttttttca
    14161 ccatgttcat actcctatct aaatcctgtg gttattttcc catgaaatca aaaagaagta
    14221 aatgaaaatc acgtgtgcca gtacttattt gctttgttgg atagcttcga ttatttgttt
    14281 cgttgagcgt actcgtgcag ttttcggaaa tatctttttt atgaacaatt gatgggattc
    14341 tttgtcacga tcactgcaag catcttcagc aagcacgata tggtacccgt gttgaaaggc
    14401 atctaatgca gtagcatata caccgtttgt tgtcgctaca ccgcttaaaa tgatagtttc
    14461 gattccacgc cggcgaagtt gaagatcaag agaagtacca aaaaaagcac ttggattgta
    14521 ttttgtgata gtcaacatat tatctgtatc tttcaaagaa tccttcaaaa gtaaatcagt
    14581 gaattctgga ggtacagaag cagtcattcc acccatatca gtttgttgac tcaaataagg
    14641 gaaagtggag atgtctacgt tcactaaaac tttcaaggca gcggtacgtt tcaatgttct
    14701 tactaacaaa tcattgtttg tcagaataga tgctgcagtg tgtggagaga gtgtaccgtt
    14761 atttacgatc tctttttgca tgtcgatcgc gacaaaagca gtttttgata aatcaaaaat
    14821 catattttcc tttaaatcat tcatgatgtt ccttctttca aataacaatg ttttttatgt
    14881 aagtaaatca taaatcgttt atttgtcaac taaattaaag gattcctttc tttagtctag
    14941 agaaattcag tggtaaaata aataagtgtg agaaaacgca ttcgatgcat agatatagcg
    15001 agaaatatcg tgaaaatgga ggaatcagag tgaagtatgt cgaagtaaaa aatgaataca
    15061 aacgatacaa aatgggagaa acgaccatta cagcaaatga cgatatttct ttttcaattg
    15121 aaaaaggcga attagtgatt atacttggtc caagtggagc aggaaagtca acgattctta
    15181 atattttagg tgggatggat acacctgatg aaggtcagat cattattgat gacacagata
    15241 ttgcgcaatt ttcagataaa cagcttactg cttaccgacg aacagatgta gggttcgttt
    15301 tccaattcta caatctcgtt cctaatctaa cagcaaaaga aaatgtggaa ttggcaacgg
    15361 aagtttctcc tgatgcgctt gatcctgtag aggtattgag gcaagtgggc ctagctcatc
    15421 gattgaataa ctttccgtcc caattatcag gaggcgaaca gcagagggtt tcaattgctc
    15481 gtgcactagc taaaaatccc aaacttcttt tatgtgatga accgacaggc gcgctggatt
    15541 ttgaaacggg caagcaagta ttgaaattat tgcaaaacgc tagcagacaa cacggcaata
    15601 ccgttttgat cattacacat aattctgctt tagcgcccat cgctgataga gtcattcaca
    15661 ttaatgatgc taaagtaagg tctgttgaat taaatgatca tccttcatcc attgatgaga
    15721 ttgtatggta gggggatttt atgaaaacaa gaacatatct taaagcaggt tttcgcgaaa
    15781 ttcggcaatc taaaggacga ttcattgcaa tcatccttat tgtcatgcta ggaacacttt
    15841 tatttgtagg tgtcaaaact gctggacctg tcatgcaaaa aacgatggat cactatgttg
    15901 gtacagcagg actttctgat ttgcaaatcg tttctactgg tggcttgaca caaaaagata
    15961 ttgctcaagc agaaaagatc ccagatgcaa aagtagaaac aggaaaacag atctattatt
    16021 ccaatactgg caaaaacgaa gtgattcaaa tattttctta taacaaaaat aacaagcaga
    16081 acaagctaca attaactgat ggaaaactcc cggaaaaacc aaaccagctt gtattggatg
    16141 aaaaagcgga agatgaaggg tataaaatcg gtgatattta tcagattgat tcagatgaat
    16201 tgaaagaaaa agagtatacc atcacgggct ttgtccgttc tccattgttc atcaacaatt
    16261 tggagagagg atacgcgaac gtcggaaatg gtagcgtcga ttattttgtc tatttacctg
    16321 aatccgtttt taaaacagat atttattctg tcttatatct tgattttaca aacgtaaaga
    16381 atttgaatac gtacagcaaa gcttacaaag ataaaatgga gcaaaatcaa gaaaaggcag
    16441 aaaagtattt acaagatcga cccgaagaac gcttagaaga gctgaaaaaa tcggccaacg
    16501 aagaattaga accggcacag caaaaaatag cggatggcaa agcacaaaca acgcaagcaa
    16561 gaacacggtt agaagatgct aaaaaacagt tagcgcaaca gtcagcaact atccagcagc
    16621 ttcctgaagc gcaacgaaca atcgctgttc aaacattatc tcaacaagaa gctcaacttg
    16681 ctgaacaaga aaggcaatta gttgagaaag aacgtgaact ggcctctgca gaggaagaat
    16741 tgaatcgaaa tgcagaaaaa attgaaaatt taactaaacc atcataccta tacaatgaac
    16801 gttcagaaaa tgtcggtttt caagaatttg gtgatctgtc tgatcgtatc gctgcgattg
    16861 ccaatgtttt tcctgttttc tttttcttta ttgccgcatt gatcaccttt acgacaatga
    16921 cacgtatggt agaagaaaac cgcagggaaa tcgggacatt aaaggcgtta ggttatacga
    16981 aagttgaaat cgcaaaaaaa tatgcaatct atgcaagtct agcctcaggg ataggtattg
    17041 ttttaggaac gatattagga acgaatcttt tgccaaggat catttttgag ctgtctaacg
    17101 agcggtatga tattgggagt gcgcttattt tttatgactg gccgccgatt ttacaagcag
    17161 ctgccgcatt cttcattgcc gcatttggcg ctgcaatgat cgttttattc aaagatttaa
    17221 gagaacggcc tgctgcattg cttcaaccga aagcaccgaa gccagggaaa cgcatttttc
    17281 ttgaatacat cactccgctg tggtcacgcc ttagctttaa tcaaaaaatc agttaccgaa
    17341 atctcttccg atataagtca agaatgctta tggcgattat cggaattgct ggatgtgccg
    17401 ggctgatggt tgcaggcgtg ggattgaaag attctctaag ttctgtttca gccaaacaat
    17461 ttggaccgat cattgactat caagcaatcg ttacattgga ttcatccacg gaagatagtc
    17521 gtcaaaaagc agcggatatg ctgcagaagc aagaaaaagt aaatgagatc ctagctattc
    17581 atactcaaac cgtagaattg agaaaaaaag gacatgcttc gcaaagtctg acagtgatcg
    17641 taccaagtaa aacagctgat ttggatcgtt ttatccattt aactgatctt gaagagaacc
    17701 cacgctcctt gcctaaaaca ggaatcgcta ttacgcaaaa agtagcagaa ctatttgact
    17761 tatctacagg agatcatatt tcgctttatg atgaagatca aaaagaatgg aaagtcgaag
    17821 taaaagaaat cgtgaaaaac tatctaggta gctttattta tatgtcccct gaatattatg
    17881 cttcagttac tgatcaatcg atgatggata atgcattttt gctgcgtaca gataagatga
    17941 caacaaaaga agaagaaatg ctttccgaga aactgcttgc cacagacgct gtgacaaata
    18001 cgtctttcat gtccaaacaa atcgaaacgc aagaagaatc aatggcgaac ttagactcgg
    18061 ttgtcttaat ctttgtcgta ttatcaggac tgctggcttt tatcgtcttg tataatctaa
    18121 ctaatatcaa tatttccgag cgtgtacgcg aattatctac aataaaagtc cttggatttt
    18181 ttgataaaga agtcacgatg tacattgttc gagaaaatat catctttact cttttaggaa
    18241 tcatcggagg atttggtgta ggatatatat tgactgactt catcttgcaa caagcgtcta
    18301 tggagaatgt catctttcca ttagtgatca catggtgggc ttatgtctta tcagcaggat
    18361 taactatcgt atttacggtt attgtgatga ttgttaccca tttcaaatta aagcatatcg
    18421 atatgattga tgcgttgaaa tcgaatgaat aaaaacagag gttgtgaaat cagcggtttg
    18481 acctgagtat tagagaaaca aacggcaaaa tagtttttca tattttgccg ttttttgagc
    18541 taataccgaa ggacagctga ttgaacacca tgaatcagag gttgtaaaaa agccgtcctg
    18601 catcgagcaa aaagaatact caaataaaat tagctcctca tgttttacat ttgagaagac
    18661 tgattgccgt agataaaagc ttttgaacac catttataga agaaacaaga atattttgct
    18721 attttaaaca agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag
    18781 acctcttaga atgtataaag ataaatttta caaacgcttt cggttcagtt ataataataa
    18841 atgataactt gtatatataa gttgtggtag gtaataaatc agttgaatgg aggtaaatat
    18901 ccatgacatt taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta
    18961 atggtgatgg aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc
    19021 taggcatcga catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt
    19081 atgatatctc tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac
    19141 agctaatagc cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc
    19201 acgtttcgac agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg
    19261 acttcttcat tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt
    19321 ggtcgccatt tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg
    19381 acttgaactg gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga
    19441 tggaaaaagg ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac
    19501 aattgaaaaa taataaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc
    19561 atacgtatct aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag
    19621 tcggtgaaat gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag
    19681 aagaattatc aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata
    19741 aatggtctac tcctccattt gattttgcag aattaaagcg actatttcat acatggggag
    19801 aaaagatgag tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac
    19861 gtgcgctcaa tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag
    19921 caaccatgat ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca
    19981 tgatcgaccc agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt
    20041 ataagttact ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa
    20101 aatctcgcga taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta
    20161 ctacagggaa accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag
    20221 aactatcttc tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa
    20281 catatccaat cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt
    20341 atggctttct tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata
    20401 aagatacaag gatcgccatc ccagaaggat tcacagaagc caatattctt atcgataatt
    20461 attccgatac tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc
    20521 tgaaaaatta aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac
    20581 gttttgtgtt ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt
    20641 tatcgccata aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta
    20701 atttgactaa cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac
    20761 ccatgacaag taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta
    20821 ctcgaggaat caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt
    20881 tgacagccat aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa
    20941 acggcccaat atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa
    21001 attctaatcc gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa
    21061 tgatttgccc ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa
    21121 aatctgtacg aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat
    21181 ctctcaaaag gaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa
    21241 cagttgaaaa aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc
    21301 cagctatatc agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa
    21361 aaggtgtttt ctccaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca
    21421 aactactgat caatgtcttt gcctgttctt caagaaaagg aataagccat atgcttccaa
    21481 gccccaataa aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt
    21541 gcttgctcaa acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata
    21601 atgggggcaa gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat
    21661 ggaaaaaata aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt
    21721 ttcctcctaa aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc
    21781 ctcctttatc tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt
    21841 tgattgcaaa aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa
    21901 cgcttgacag ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt
    21961 acggtttgcg aaaagcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca
    22021 taaatgaaac gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct
    22081 tgcggggcaa gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt
    22141 tatcccatgt atgattttac aaaagcaatc gtcggggatg aaggagaagt agagttattg
    22201 attccagcag ggactgattc ccacgattat gaaccttctg ctaaagacat ggcaaagatc
    22261 caagatacag atatctttgt ttataatgat gaaaacatgg aaacatgggt ccccgcaatc
    22321 caaaaaacat tacaagaagg caatgtccat acgataaaag cgacagaagg tatgctttta
    22381 cttccgggaa gtgaagaagg acatgatcat gaccatgaac acggggaaga aggccataca
    22441 catgagctag acccacatgt atggctagct ccaagtttag caatcaagca ggtagcaaac
    22501 atacgtgatc agctgattga agcttaccca gaaaaacagg aagtatggac aaaaaatgca
    22561 gcagcctata ccgagaaact tcaagcactc catcagcttt atcaagaaac ctttaagcaa
    22621 gcaaaacaaa gatcttttgt cacgcaacat gctgcattca actatctagc gttagaatat
    22681 ggattgaatc aagtatcgat tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga
    22741 attgctgaac tgaaacactt tgttaaagaa catggaatca actatattta ttttgaagaa
    22801 aacgccaaag atagtattgc cagaacgtta gcaaacgaag caggtgtatc tttagaagta
    22861 ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa atggagaaaa ctatctttct
    22921 atcatggaag caaacctcga agcgttgaaa aaaacgacag agacagaaaa cccgctagaa
    22981 gatactttga cccccaaaaa agaaaagagc gtctacaacg gttattttga agacgaggat
    23041 gtacaagatc gctctctttc agattgggca ggaacttggc aaacagtaga tacgtatgta
    23101 gaagatggga cttttgatcc tgtttttgaa tataaagaaa aattgaatca ggataagaca
    23161 gcacaagaat ataaagaata ttatacgaag ggctatcaaa cagatattgc ttcaatcaag
    23221 atcagtgaga aagagatggc atttacgttt aagaatggaa caacgaaacg ttcaaattat
    23281 cgttatgtag ggaaagaaat cttgacctac gaggcaggaa accgtggcgt aaggtatcta
    23341 ttcgaagcag aagataagga aagtggcgct tttagatata ttcaattcag tgatcacacg
    23401 attgcaccgg ctgactccga acattttcat ttatatgcag gaaa
//
LOCUS       DAZUKA010000030        23386 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000030 DAZUKA010000000
VERSION     DAZUKA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.030"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..774
                     /locus_tag="ACG8PN_000938"
     CDS             148..774
                     /locus_tag="ACG8PN_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4861113.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            797..1501
                     /locus_tag="ACG8PN_000939"
     CDS             797..1501
                     /locus_tag="ACG8PN_000939"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4861114.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            1693..2526
                     /locus_tag="ACG8PN_000940"
     CDS             1693..2526
                     /locus_tag="ACG8PN_000940"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4861115.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            2723..4651
                     /locus_tag="ACG8PN_000941"
     CDS             2723..4651
                     /locus_tag="ACG8PN_000941"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4861116.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            4789..5244
                     /locus_tag="ACG8PN_000942"
     CDS             4789..5244
                     /locus_tag="ACG8PN_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4861117.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            5324..5452
                     /locus_tag="ACG8PN_000943"
     CDS             5324..5452
                     /locus_tag="ACG8PN_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861118.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            5714..5983
                     /locus_tag="ACG8PN_000944"
     CDS             5714..5983
                     /locus_tag="ACG8PN_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861119.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            6383..6637
                     /gene="rpsT"
                     /locus_tag="ACG8PN_000945"
     CDS             6383..6637
                     /gene="rpsT"
                     /locus_tag="ACG8PN_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4861120.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(6726..7772)
                     /gene="holA"
                     /locus_tag="ACG8PN_000946"
     CDS             complement(6726..7772)
                     /gene="holA"
                     /locus_tag="ACG8PN_000946"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4861121.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(7810..10083)
                     /locus_tag="ACG8PN_000947"
     CDS             complement(7810..10083)
                     /locus_tag="ACG8PN_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4861122.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(10076..10615)
                     /locus_tag="ACG8PN_000948"
     CDS             complement(10076..10615)
                     /locus_tag="ACG8PN_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4861123.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(10751..10969)
                     /locus_tag="ACG8PN_000949"
     CDS             complement(10751..10969)
                     /locus_tag="ACG8PN_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4861124.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(10966..11211)
                     /locus_tag="ACG8PN_000950"
     CDS             complement(10966..11211)
                     /locus_tag="ACG8PN_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4861125.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(11327..12055)
                     /locus_tag="ACG8PN_000951"
     CDS             complement(11327..12055)
                     /locus_tag="ACG8PN_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4861126.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(12214..13257)
                     /locus_tag="ACG8PN_000952"
     CDS             complement(12214..13257)
                     /locus_tag="ACG8PN_000952"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4861127.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(13247..13738)
                     /gene="coaD"
                     /locus_tag="ACG8PN_000953"
     CDS             complement(13247..13738)
                     /gene="coaD"
                     /locus_tag="ACG8PN_000953"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4861128.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(13735..14295)
                     /gene="rsmD"
                     /locus_tag="ACG8PN_000954"
     CDS             complement(13735..14295)
                     /gene="rsmD"
                     /locus_tag="ACG8PN_000954"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4861129.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(14414..14752)
                     /locus_tag="ACG8PN_000955"
     CDS             complement(14414..14752)
                     /locus_tag="ACG8PN_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4861130.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(14736..15182)
                     /locus_tag="ACG8PN_000956"
     CDS             complement(14736..15182)
                     /locus_tag="ACG8PN_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4861131.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(15163..16287)
                     /locus_tag="ACG8PN_000957"
     CDS             complement(15163..16287)
                     /locus_tag="ACG8PN_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4861132.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(16308..19736)
                     /locus_tag="ACG8PN_000958"
     CDS             complement(16308..19736)
                     /locus_tag="ACG8PN_000958"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4861133.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(19762..20925)
                     /locus_tag="ACG8PN_000959"
     CDS             complement(19762..20925)
                     /locus_tag="ACG8PN_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4861134.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(20928..21230)
                     /locus_tag="ACG8PN_000960"
     CDS             complement(20928..21230)
                     /locus_tag="ACG8PN_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4861135.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(21344..23116)
                     /gene="recQ"
                     /locus_tag="ACG8PN_000961"
     CDS             complement(21344..23116)
                     /gene="recQ"
                     /locus_tag="ACG8PN_000961"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4861136.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         6793 a         4773 c         4106 g         7714 t
ORIGIN      
        1 tccttctctt tagaatatcc aaatactttt catatagtct gataattcag ataagtaaag
       61 agaaataagt acctgttttt gtttcctagc aatgaatgcg ttatactaat ggatgaaaga
      121 agttttggct atcaaggagg aatgcacatg catatcgaaa gattaaaaac aggaatgatt
      181 gaagaaaact gttatttggt ctacaacgaa gaagcacttt tgatcattga ccctggagaa
      241 gatgcggaac gaatcaaaca agaaatcaaa aaaacaaacc aacagccggt agcgattttg
      301 cttacccata ctcactacga tcatatcggt gcagtggaac ctttacgcgc gtattatcaa
      361 atcccagttt atgtcagccc actagagcaa aaatggctag gtgatccgat tttgaatctt
      421 tctggactcg gtagacatga tgatattgct gatgttattg tctcgcctgc agaatatgaa
      481 tttgaaatga aaacttatac tttaggaaac atgtcttttc gcgtcgtccc taccccaggg
      541 cattctatag gaagcgtcag ctttattttt gatgattttg tcgtttctgg cgacgccctg
      601 tttagaggaa gtatcggtag aaccgatctg tatacaggga atttagaaca attacttcat
      661 agtattcgga cccagctgtt tgtattacct gatgaatttg cagtctatcc aggacatggc
      721 gatgcaacga cgattgaaca ggaaaaaagg acaaatcctt ttttcaatcg ttaaaacgac
      781 ttaggaggag aataaaatga cagaaactac actttatatc gtacgacacg gaaaaacgat
      841 gttcaataca attgaacgag tacaaggctg gtgtgataca cccctcacaa agcaaggaca
      901 agaaggcatt cattatttag gtcgcgggtt aaaaaatgtc gacttttcat ttgcttattc
      961 tagtgacagt ggtcgtgcaa tcgaaactgc ccgtattatt ctaggagaac attctaaggg
     1021 aaaagaaatt ccttatttta ttgaaaaaag gattagagaa tggtgtttcg ggtctcttga
     1081 aggtggatat gacatggaaa tgtggggtgt gatccctcgt gtattgaatt ttaaaacata
     1141 tgatgaaatg tttaccacag aagtttcttt tgaacaaatt gctaatgctg tatacaactt
     1201 agataccgct gggtgggcgg aaacttatga aacattgcga gaccgtgtat ggacaggatt
     1261 tgaagatatc gcccatcact gcgaaaaaaa tggtggtgga aatgttttag tcgtatctca
     1321 tggtttgacg atcgcctttt tgcttaatct gatcgatcct aaacagcctg ttcgtgctgg
     1381 gcttttaaat ggcagcgtaa caaaggtcac ttatgcaaac ggtcgatttt ctatccacgg
     1441 catcaatgat acgtcttata tcgataacgg gaaaacacat agtcgcgact cgtttctata
     1501 atggggatat tttaattttt accagagaat cataaaaggc aaagtctgaa cgagggcttt
     1561 gtcttctttt ttctcttcgt gtacttatct gctttatttc tcaagttttt cttaatatta
     1621 aaaaacagat tgggtttctc aaaaaataac ggtatactat aagatgtatc ttaattggga
     1681 aggaaggaat ttatgttttt tgcaaatatt atcaaagcag tgattctcgg tattattgaa
     1741 ggaataacgg aatggctgcc catcagtagt actggtcatt tgatcctagc agatgaattc
     1801 atcaaactgg atgccagttc acaatttatg tcgatgttca atgtcgttat ccaattaggg
     1861 gcgatcttgg ctgtcgtcgt tctttatttt cacaagttaa acccattttc tccaagtaaa
     1921 tcaacggtag aaaaaaaaga tacgtggaca ctttggttta aagtgatcgt tgcttgcata
     1981 ccagccatca tcattggttt gcctttggat gattggttag aagcaaattt ccacaagttc
     2041 ttaccagttg ccattgtttt gatcgtttac ggtattgcct ttattattgt cgaaaaacgc
     2101 aacaaaaaca aaacacctcg ttggacttct ctgaatgact ttacgtacca agcagctctt
     2161 cttgttgggt tattccaagt cttggcactt gttcccggaa catcccgttc tggcgcaacg
     2221 atcctaggat ctatcttgat tggtgcttcg cgttttgtcg ctacagagtt ttcatttttc
     2281 ttagggattc ctgtcatgtt tggcgcaagc ggcttgaaga tcgtcaaata tttagctgaa
     2341 gggaattcat tcaaaatgga agaaactgtt gttcttcttg taggaacatt cgtatctttt
     2401 gtggtttccg tcttagctat caaattctta ctaagctatt tgaagaaaaa cgactttacc
     2461 gcttttggct ggtatcgtat tatccttggt atcattttga ttggttattg gtttattgca
     2521 atgtagaaaa aaccagaaca cttctcttat ttgagaaatg ttctggtttt ttattttctt
     2581 taataataat cattataaat aataaaaaca acatcaagct taatcaccaa atgataacca
     2641 ttatcgttct catttagctg ttattattat caattacatt tttattatgc tatccttgtt
     2701 ttgacaagga ggtctaataa cgatgactaa tcagaaaaaa gcttttgcag caacaatcgg
     2761 ctgcctttta tttatgatct tcggttttat cttcgaccga ttctctctta ccatcacccc
     2821 acttttttat atcagtgcca ttatcatcgg tggatataaa caaacatcgg aaggcttgaa
     2881 agaattgtgg aatgatcgga cactgaatgt ggatttattg atggctttag ctgctattgg
     2941 tgcctgtttg atcggtaatt actttgaagg agcaatgttg acattcatct tttgtttaag
     3001 cggtgcatta gaggaataca caactaataa aagtcaaaaa gaaattactg ctttgatgaa
     3061 tcttcaacca caaaaagccc aattattaaa agaaaatgga caaatgcaag aagtagatgt
     3121 ttctcaacta aaggtggata acttggtatt tgtcgctaaa ggagcagctg ttccgataga
     3181 tggcttttta gaaagcacac aggcaacgat cgatgagtct gcattaagcg gtgaatctgt
     3241 gcctgtagaa aaatatttag gcgatgagat gtatgcagga acgctgaatc aaggaaatcc
     3301 catcacgatt cgagtaacaa aaacgagttc cgaaactgtc tttgctaaga ttatccaatt
     3361 agtagaagaa gcgcagaata cgccaactca aacagcttca tttatcgaac gaatcgaaaa
     3421 taattatgta aaattgattt tgcttgctgt tcctctaatg atccttctcc cccacttttt
     3481 cctaggttgg agttgggatg aaagctttta tcgaggaatg gtattattag ttgtggcttc
     3541 tccttgtgcg cttgtcgcat ctgctacacc agcttctcta gctgctcttt caaattctgc
     3601 aaaaaatggg attttaatca aaggcggtat ccatttagaa aagctttctg aattaaaagc
     3661 gattgccttt gataaaacag gaacgctcac taaaggaaaa ccagttgtaa ctgacagctt
     3721 tttctttgat gaaaaggatt tggcacaaca acttcttgtt gctatggaac aaaaaacgac
     3781 gcatccactt gctcatgcga ttttgcaata tttcgattgc acaatacctg aagaaatcca
     3841 atacttacca atagaagaaa ttactggttt tggattacaa actacctatt taggtgcgca
     3901 atggaaagtt ggcaagcacg catatgatcc tgagacgatg ataatcagca aagagattgc
     3961 ggaaatgatc gaacgtttgg aaaatcaagg caaaaccgtg atttatcttt ctaaagatca
     4021 gcagttaatt gctgttttag gtctgctaga tattccaaaa gcaaataccc aacaagtaat
     4081 ttcctacttt aaatcccaaa acatccatac aagtatgata accggcgacc attctggcac
     4141 agcaaaggcc attgctgaac aagtagggat tgaacattac tatgcaagct gtactccgga
     4201 agaaaaaaca caactagtca aaaaagagaa tgaactttac caagtaaatg cgatggttgg
     4261 tgatggagtc aatgatgcgc ctgctttagc agccgcttct ttaggtgtcg ctatggggca
     4321 agggacagat gccgctatgg atatcgcaga catcgtctta atgaaaagcg acttaaataa
     4381 atttgtctac agtcatcggc tatctttaaa aatgcggcgg atcatcaaac aaaatattat
     4441 tttttcgatt cttgttattc tcttgttgat tgcttctaac ttcctgcaat ttttaaatct
     4501 acctcttgga gttgtaggac acgaaggaag cacaatattg gttattctga atggcttgcg
     4561 tttactaaga cctttacctg atctttctga ttcttcaaaa aaatgtaagt cttgtccact
     4621 atacaaagcg cggctgtctt ctgtcaaata agcaaagtgt ttttttatga ttttgtttat
     4681 tttatgaaaa aaacagctta tgaataggtc ttgaaggatg aattcctact tttgtaactt
     4741 tctaaaaaca tgttatgatt gtttcgaaga aagtgaggaa tatatttcat ggaaagttgg
     4801 ctttttttag gaattatttt acttgtagca attttagcta aaaatcaaag tctgatgata
     4861 gcggcagctt ttgttttgat tatgaagctc ataccagcct cccgttcctt tttcccttgg
     4921 cttcaaggca aaggaatcaa tattggggtg acgatcatca cagcggctat tctaatccct
     4981 atcgcaacag gagaaatcgg tctacgtgat ctattagaag cctttaaatc ccctattggc
     5041 tgggtagctg ttttttgcgg tgggctagtg gctgtcttat cttccaaggg tgtaggattg
     5101 attgcttctg atccacaaat cactgttgca cttgttttcg gcacgatttt aggtgttgtc
     5161 ttcttaagag gaatcgccgc cggaccggtc attgcttctg gtatcactta ctgtatctta
     5221 caagtatttg ctttaggaaa atagaacaaa ttttgtctat tttaggagat tcttaacacc
     5281 cccaatttac tgatccgtga tatactgaca acgaggtgat acaatgttcg aaaaattaaa
     5341 gtatttcata ggaacaatcg gctggtttct tctagcggta cttttgattc tgggcttagc
     5401 ttatctatta tttcctgatt tgatacaaaa tgtgtttgat actgcttttt gattgaccta
     5461 ttaaaacgat ataggggatg ctcctgcgat gcaggggctt tttttaatca atgttttgta
     5521 ataatttagg ccgtgccaat gagacttcct atagaattcg atgctctttt ttctttttgg
     5581 ctcttctgct cttttttgtt atactagatt acagtggaca aaagaaagaa tgatcgcatc
     5641 cacttcaacg aatatcaaaa tggaagaatt agtgcctgtt ttcaggttac tttgaaaaga
     5701 aaggaagacg cttgtggaaa cttttgaagg tcttttaatt gatacgtacg ttttacaaga
     5761 acagccaatg atgctgactg ggatcaactt gcagcttgag gaccgtgtga tcaatggcct
     5821 gcttaaagat cgctatttgg caaaatatat cttacgtttg cccctaaata aatatgtgct
     5881 ggaagtaagt ggcaaatgga accgtcggaa ccagttggtc ataaaaaaaa tgttgataaa
     5941 aaatatcgat gaatacatta aaataatcgg tacaccagaa tagcctttac tgatcaaagg
     6001 atactggtta taaaataact gcagtttctt aatagaaaaa caaagaacaa cagggaatcg
     6061 tatccctttt caaaaaaaac tttgcaaacg gttctctttg ttatataata acttagtacc
     6121 aaacattccc attcaaactt tgtttaaata gataaataaa aatctagttt ggtttgacgc
     6181 tattgcctcc cacaatagcg tctttttgat ttttttcagc ttgtactatc cgtttttact
     6241 gaaaaaaaat cctaataaaa gactgttaag gaaaaaaact tgtcatgttc atattgcaaa
     6301 gcacgctgcc tttaggtata ataatgaatg ttgaattatg cggtaacacg cgtaagaact
     6361 taagttatcg gaggtgaaac taatgccaaa cattgaatct gcaatcaaac gtgttcgtac
     6421 aagcgaaaac gctaatgtga aaaattcatc tcaaacaagc gctatgcgta ctgcaatcaa
     6481 aaaatttgaa gatgctgtag cttcaggtgc tgacaacgta gatgcgttat ataaagaagc
     6541 tgttaaagct attgatatgg ctgaatcaaa aggtctaatc cacaaaaaca aagctaaccg
     6601 cgacaaatct cgtttaagca aaaaaatcgc taaataaaag aatcggcttc ggccgatttt
     6661 tttatttgtc aaaaaaagga caaaaacaaa cgaccttccc gcatgctttg ccctcgtctt
     6721 tcttcttatc gatttcttaa caactgccct gtgcgcaaaa cgaatagttg aaaacttaat
     6781 tctttttcga tttgtcccgt tttgatttga taatccatct caactaattg atcaaacaac
     6841 tgtaccagca aatgttcatc gaattttttt acttcctgca tggcaagttt tacacgatat
     6901 ggatggattt tcaatgtttc agcaatattt gcctgctgat aaccaatctt tacaagaaat
     6961 tttgtctgta aaaataaacg taattgagaa agcaaaatcg cattaatttt gattgtttct
     7021 tccccttgca taactaaatc ttcgtataaa cgcaaagctt gttccgtctt cccttttaaa
     7081 atatattggg tcatgtcaaa aatattatgt tctaacgttt taggaactaa ttcttctact
     7141 tctcttaccg tgatttgctg ttgattttgt ccataaagga ccaatttctg taactctctc
     7201 atcattttgg ataaatctaa gtcggtcaac ctcaagaata aatcaattgc acttcgttcc
     7261 attttgattg aggaattctc taaggtattc atcagatatt gacgtacttc tttttcattc
     7321 aactgttgga cattgatgat aattgctgtt tttttcaatt gtttcgttac ttttttccgt
     7381 tcatccaact tttcatatgg cgcaaagaat actaatacag tcgattctaa tggttctttc
     7441 aaataagccg tcaataaatc agtattttgt tcgattccat tgtttacttt ttcacctgtt
     7501 aaaaaataag gattctctac gaatactaac cgttgatctc cgaaaaacgg caacgtctct
     7561 gcttcagcta ctacctcatc taaacttgca ttttccatgt caaaacttaa aaaatttaag
     7621 tcatcttcgc cctctaattg gatttttttg acaatttctg tccgaacttg atcttggaga
     7681 aacttttctg ttcctaaaat caagtaacaa gatgcaagtt tttctttacg tattttttgt
     7741 aactctgctt gtatactcaa attactcacc actttcaaaa attatcctac catgagcaga
     7801 tgagaattac tagtctatca ttgtttccac tagcccatga ggatgaaaaa ctggatgcca
     7861 gcgataggtg atcattcctt gttgatccgt tcgccatgtt tttatctgat tttcttctag
     7921 tgtctccaat acttcgctgt gcgggtgacc ataacgatta tttacgccgc aggatataat
     7981 cccatgtttt gcttcaatat gggaaagaaa atctgtgtta cttgacgtac ggctaccgtg
     8041 atgacctaat ttgactacat cggctttcag attaggataa atcaaaagca attgtttttc
     8101 cattgcttga ttcaaatctc ctaaaaataa aaagcgccta ttgttatatt ccatccatag
     8161 accaatcgaa tcttcatttc ttccctctcc cttttcaaaa ggagtcaata cataaagatc
     8221 ataataaccg ctgatcctgt ccccatgttt gatttcaatg atctcagtac catttttttg
     8281 agcttcagat aaatgccttt gtaatggctc atttcgtcca gcgccccaac cgatataaat
     8341 ttttttgata gtaaaatgct gagataacgt gggtaattct cccacatgat ccgtatcgtc
     8401 gtgtgtcaag atgagtttat cgattacact tatcccctga ccttttagat atggaacgat
     8461 atttctttca gcaggaggac gtatcatacc ttgcgcccac ttttctcttt caaagtgtaa
     8521 ctgacctcca gtatcgatca aaatcgtttc ttttcgaaaa ggactttgca aaaagatact
     8581 gtctccttga ccgacatcga taaaagcgat cgttgaagaa aagcgtaaaa atggcaaagt
     8641 caaaaaaata aaagaaaaaa gaccaacata aaacaagtgt ttcacaggtt gttctttcaa
     8701 cctatccata caaatcagta atccgaaaat atacatccca gatagcacaa ggggcaaacg
     8761 tcctataatg agagacgacc ctgaagaaaa atcgattatt cgctcaaata agattaatcc
     8821 ctcttctatc agcaaaagta aagtttccgg cagccagcga ttgaagagtg tgagaaataa
     8881 cacaccagga agaaaaagaa acttgaatac cggtgcaatc agtaatgtaa aaagactacc
     8941 taaaataggc cactcataaa aatgccatac aatcaacggc atcgtgatca tagtaaacag
     9001 gacgtgagag gccgtttttt tcaaccaatc tccatcaaaa gaaatcagga taaacaaaaa
     9061 actcatgaaa aaactaagct gtccacccgc atgaaaaaga aatcgaggat caaatagtaa
     9121 aagaagccaa aagacaactg caaataagtc catggtggaa taatgcttat tcagcaattt
     9181 gactagcaat cgcaataaaa acagcaaact agcacgcaaa acagaaatag aacttcctgc
     9241 aatagaaaca taacagatag ttagtagact caacggaata atactgacgt gtagtggcca
     9301 tccgatacgt cgaaacaaat aatacagcca ccctaaataa aattggatgt gtagacccga
     9361 tagactgaac agatgcagca aaccactctc cctaaaaatt tcttctggtt ctctgaatcc
     9421 tgcatcttta tagccccata ataaagaatt aatatataca cttgtttttt gcggaaattc
     9481 agattggaca aaggagataa aagatcctct ttttctctga agccagtttc gccagtgatt
     9541 ttgcatggtg atctgttcaa tcttttgaat ttggaaccgt ccagaaattc tatggttcct
     9601 gaagtaagct tcttggtcaa aattataaag attccgcttt tttgctggag agatgaactc
     9661 gccttcagca gtcaattcta ttccctttcc tcggaagtta agccatgttt tcaattctgc
     9721 ttctgtgttt acttgataag aaagctggag cttttgttta ttctctgata accttccttc
     9781 cattgtcaaa aaattaccat ttgcacgaat cgtatctgta aatacagtaa tttgcgcaga
     9841 tgttgttttt tcctctaaaa atgaatactc ttgtagccta taagaaatgg ataccgttat
     9901 gctacacaaa atacacaagt acgcgataaa acgtccttct tttagcgaaa accataaaaa
     9961 aattccggca agaagcaaac gataacccca attgtcagtg taatacaaca aacagataat
    10021 catcgacgta attgccggaa aaatcaaccg atttcgaaca tgcctttcta aaacgctaga
    10081 catgttttcc tgttaattca ttttgaagtt ttgaaaaatg ctgtgagtct aaagcaactt
    10141 ggtgtacaga tacacctaat tgttcgatca acgcttgggc ataaggatca tttcgatagt
    10201 catggagata attgatccct ttcacaccag cttgcaacaa agcctttgta caggccaaac
    10261 atggaaaatg tgtgacatag atttctgaac cttccgtcgg gataccaagt tttgcacact
    10321 gtaggagtgc attcatctct gcatggatcg ttcgcaagca gtgtccatcc acgacgtaac
    10381 acccttcatc tatgcagtgg acatctccac tgactgctcc attatagccg ccagcaatga
    10441 tccttttttc tcgaacgagt gtcgctccta cttctaaacg tgtacacgta ctacgtaaag
    10501 ataacagtac tgcctgagcc ataaagtact ggtcccaagg aatgcgttca ttatgtgact
    10561 tatcagtaac tttttcttta ttcgtatcgc atgcttttga agatttttct aacatgtttt
    10621 atattcctcc gttttttcaa gttgattaaa aagtactcat tgcttaaaca aaatattctt
    10681 taaatataac gaatcatcct gtctgacaaa agatcctacc cttcactgcc gaaatgaagt
    10741 ccgtcatcca ttagcccatt ataaaaattc ttcgaatcca tttgttcaag aaattccgta
    10801 taaaaatcga tacttcacaa tcatggcgtt ttgctacttc tgtgagaaca gagatatatt
    10861 ctttgatcaa cttgttattc cgatattttt gttttgtctc atcgactgct ggtggcgtga
    10921 taaagaaagc atgatcagaa ctatagtctt ttttcagctg aactatcatc cactgcagat
    10981 tttttggtac gtttctttag aaagttgttt atgctctgct agatcattcg tcccaatgag
    11041 aataaatact ttatctgctg tatagctaag tacatctttg gcaaagcgtt ctcgtagagc
    11101 ttgactgtta ttgccgctga cagccgtatt cataatatga atcccagata ttttattttg
    11161 gagatagaca ttcagcattg gtttttttaa tgtttctttg cgaacaatca agctatttcc
    11221 tatccaaaaa tactacctcc tcgtcctgcc aagctcttag aattggatta ttcttagtat
    11281 ccattagcgt ccatttattg ctcgtgccag tcgattttga atcattctat ttaattgtaa
    11341 cgaagttttt tagtttttca actgtctttt caccgatacc agaaatctcc tgcaggtctt
    11401 ccactttctg gaaggatccg ttttcttctc taaatcgaat gatttcttgc gctttgactg
    11461 atccgatccc aggaatctgc tgaagttctg atggagtggc tgtattgata tttattttgg
    11521 gaggctcgga atctgctgta ggctgttttc cttccgagga tgccaactga tcaagatgtt
    11581 ctggcagctc ttcacctaca gcagggatat ataggacttg ctgatcttct atcttcgcag
    11641 aaaagttgat tcgatcgcta tccgcttctt ctaataatcc acctgcacgc tttagtacat
    11701 catagacccg ttcttctgaa gaaaaggaat agataccagg ctccttcaca cttcctttga
    11761 tatcggtgta catgaccttt ggacgagcat cctcttctac atcgtttcct tcagctgatg
    11821 gatcttctcc actggaacta aacgaagaaa gtatttcttt tgcagcagtc tcttctgttt
    11881 ctctataaaa tagcaaattt gataataaaa aaccacttgc gatacaaaag ataagaacta
    11941 tcatagacat ccaaatgatc aatttgggtg aagctagtat tttcttgctg attttttcta
    12001 tcattgttct tttttcaaag aatgaccagt aaaaatcatt cattactcct ttcatttaaa
    12061 attgaagaaa cagtgagctc tggcaaaatc gtcccttcaa catctaatta cgtcaatttt
    12121 ttcttttgtt tttattttta tcgtgaagat ttttgacgaa aaagaggggt tgtgacagaa
    12181 ctcgtgtcac aacccctctt taattattta gtttcatttc attttctcta gatagtcaag
    12241 cgcttcttgt actgttttta ctggtacgat tttcattgag gaattcaatt ttttagcggc
    12301 agcttttgct tcttcgtagt tggtttttat ttcaggatat tctttcttaa catcgtctga
    12361 aatcgtatca tctggtgcaa agaaaatctc tacgccagct ttatccgcac ttgccacttt
    12421 tttatcgatt cctccgattc ggccaacttc tccttcactg ttgatcgttc cagttcctgc
    12481 aatttgctta ccatgtcgca agtttttacc agtgacttgt tcatagattt ctaaagtgaa
    12541 catcaatccc gctgatggcc caccgataga gccagaatta atacgaacat ctgtatctga
    12601 ttcgatctcg gtatggtcag taagcgtgat acctattcct gcctttttat ctgttggtaa
    12661 ctcgatcaga tcaccagaag ctgttttttc ttcaccatta tgcaaataac tgatttcgat
    12721 tgtttgtcca actttttggc tttttacata atccataaat gcttctgtac tttgaaagct
    12781 tttcccgtct acttttgtta ctgtatctcc aactgaaagc ttgccaaaga aattcgatgt
    12841 tttctctaca ctcatgacat atacgccttt gaattccatt ttataaggct tgtctgcaag
    12901 ttttaacgct tgttcgattg ctgcattttg ggaagaatcc atataatact gttgcatttg
    12961 attatattct tcatttgtcg ccccaccagt caattcttct tcactgatca attcttcaaa
    13021 aggggtcatt ttagctttta aagctaagaa acctgtagct tgcctgatac caacagaggt
    13081 aagagagaaa gacccagaac ctttatcttc cttatcattg accgtaatta gttctttcag
    13141 atcgatcgtg gctcccggtg attctacata atacggcaag ggaactacta caaatccaat
    13201 gatcaacatt ccaaatatga taatcagtac tgttttcaac cattgtttag ctttcatttg
    13261 attcacgctt tccagctaaa gcttcgttga tcactggcgg taagtagctg ctcacatctc
    13321 cgccaaagtg cagtacttct tttaaaatac tggaactcac gtgactatat tctggttttg
    13381 ctaataagaa gacagtttcc aattcatcat atagatgatg attcatctga gcaatctctc
    13441 gttcatattc aaagtctttg atacttcgta tgcctctgat caacgcatct gcaccaatct
    13501 cttttgctgt ttctaccgtc aattgatttt cttgatgcat gacttttaca ttaggaagat
    13561 gagaaacagc ttttgtgatc aatgtcattc tttcttctgg gggaaacaaa cttttctttg
    13621 aagtattgat aaaaacacca attactacct catcgaataa tttagccgca cgttccaccg
    13681 tatctaagtg accttttgta aaaggatcaa aacttcctgg gaataatgca cgtttcattg
    13741 atttccctct tttctataga tagttacggc cgttatgcca taaatctgtt ttcttatttg
    13801 atggaatcca gccacagttt ctggcaattc gactgtttta tccgtttcgc atacgataac
    13861 tgcggtttta gaaaaaagtc ccagttcttc cattttatgg atttgatcga ttattttttg
    13921 ttttgcatat ggcggatcaa gaaacaagta gtcgaattgt tgtttctccg ctgctagccg
    13981 ttcgattgct ttgtccgcat caagttttat tgtggtaaat ttctctggtt ctttcgtaat
    14041 ttcgatgttt tctttgatga tttttagcgc ttggtaattt ttgtctacgc ataccgcatg
    14101 actgcagcct ctagatactg cttcgatagc taaaccaccg cttcctgcaa acagatccaa
    14161 tactgtctct ccatcaaaat atggtccaat catattgaat atggattctt ttactttatc
    14221 tgtagttggt ctggtgttcg cccctgctaa actttttaaa cggcgcccgc cataatttcc
    14281 tgatattatc cgcatctact cactccttag aaatggtact ggtactagta taccaaaaaa
    14341 atacttggac aatgatcaaa aatcgatcgt tatccaagtt attttttatt taaaaactgc
    14401 tcataagaga tttttaaaga tgttcagcta atcggatttc tgtatttagc tcttcaattt
    14461 cgaaaccttc gtcttttaat actgttttgt ttcctatttt ttcggcaaaa ttcatttcga
    14521 tatccggtcg gtaagaacgt tctacgtttc gaacaaaatg taatttacgg attcgttctt
    14581 ctgtttcttc caatgtctct tcatttaagt aaagcacgac atatttcatt cttcttgaga
    14641 cataatgcaa caagccgaac tttttcagat tctttaattg ttttaagctg tagacccata
    14701 cgatcagccc gcgtcgtttt gttatttcaa attcactatt gtctaattgc atgacaatta
    14761 cctccgcatc caaagtgttt tttcgtttca aaaaacgggc tacctgcatc tactttgatt
    14821 tcattagaaa cttcttcggc aagtacttgg acaatttgat ccaacagact ttgcagctgt
    14881 gtttcactat atcggaaagc tgcaacatgc tcatccatat caagctgtct ttttgctttt
    14941 cgaacagctc gttttttttc acgataatca ggtgcatact caccatatgc cgctacattt
    15001 tcaaatgctt ctttcttaga caagaacgct tctttcaact ggcaaacttt tgcagattct
    15061 tccattgact gtttatgttc aagataagaa gccatcgttt tactcttttt tatcatttca
    15121 gcgacttgga ggcattgatc ctccagttga aacagctctt cgttaacgat cactgctatc
    15181 actactttct ccgttttctt cttgcaataa gactaacata ttttctttag aaaaagcaat
    15241 gcctacagaa tccgttcccg ggtccatgaa acggtcatgg ttgtaaggat cgctgtacca
    15301 atataaaaac gaaaaagtag cgtcaatcgc cggatgaata aatagcccac tagtattttc
    15361 taatttttgt ttactgatta acttctccaa ttcatcattc gtcagtgaga atttcactgt
    15421 cgtttgagca ctgcctagcg atttttgcca atcagccata cgttcctctg acaatatttc
    15481 ctcaggcctt gtcaaaaaat catgtagtag cagctgactg tggttttgaa aggcaagtgt
    15541 ttgattgtat atgggaagtt cgtcttctct tctaagcata tttaataaag tcgtactttt
    15601 tcgttcttta acgcgattga ttgattccca atcagccgat ttatctgctt gataatgtgg
    15661 aaggccttca ccaaacactt gatatggcaa tagtttcaaa agactatctt tatctaaata
    15721 cacaactgaa aataggccgt ttgttgtttg atcaaaaaat aaaatagcaa aagtgtcgtt
    15781 atcaaaagca attagcggac gataattcat atcctcttcc attagttcga ttccaacttc
    15841 tgtatcatgg tactcaaaag tgaaattagt atagatagta gtgattttcg taagatcttg
    15901 catcgtcatc cctaaataaa acggcgcgat atcagcaggc cctgtttgca gtactttaac
    15961 agttgtgact ctgctgtctt ggatgttcgc ttcaaatgtg agatgactag ctttattttc
    16021 ataatatctt gtttcaaaac caaatccgct agttaatcga tcataggctt ggccaaaatc
    16081 cttttccagc tgatcgatcg gttttccgat ataagtagaa aaaccagttg ctttaagctc
    16141 ctggtatttc cacgttgtca tttggtttga tatttgttga tcagagtttt ccttcacttc
    16201 aagttttggc ggaaacaaga ctggttccat gtagaatgcc acaataacta atagcagaat
    16261 cagccaaaac aaacccagtc ttttcatgtt tccccacctt tcgcttatta tttctcagtc
    16321 acttcaatca aaaggtcacc agacgaaata gtgtctcctg aagacacata tacatgtgcg
    16381 acttctccgt caaaacgtgc ttcgatggcc gtttccattt tcatcgcttc agtaatcatc
    16441 aacgtatcac cttttttaac gttatcgcct tttttcacta atacatccaa aacagaacca
    16501 gacatcgttg ctccaatttg ttctcggttt gtcggttcag ctttcaatcg ggtttggacc
    16561 gtactgatga tcgaattatc tttgatagtg atttcacgtc tttgtccatt caaattgaag
    16621 aacagtacgc gattcccttc gatatcgggt tctccgattt catctaaacg aatgataagc
    16681 gtttttccgc gttcgatttg tacattgatt gtttcaccca aacgtatacc ttggaaaaat
    16741 gttggtgtgt ccagtaatgt aacatcgcca aattgattat atgctttttg atagtccaaa
    16801 aagacttgcg gatacatcaa atagcttaat acttcgtctt gttttggttc atagccaatt
    16861 ttttctgcca attctttttt tacttcgttg aagtcgactg attcagccaa aagacctgga
    16921 cgttcattca atgcaggtct gcctttaaga ataatctttt gcaatttttt aggaaatccg
    16981 ccaacgggtt gccctagttc tccttggaag aaagtcacaa cagattcagg gaaacttagt
    17041 gtttcgcctt tttcataaat atcttcttct gtaagatcat tttggaccat aaatagtgcc
    17101 atatctccaa ctactttaga tgatggagtt actttaacga tatcgccaaa catcaagttg
    17161 acagtatgat acatttgttt gatttcatcc cagcgatgac ctaatccgac agcttttgcc
    17221 tgttgctgta gattcgaata ctgtcctcct ggcatttcat gcatatatac ttctgtttca
    17281 ggagcattca agccgttttc aaacggtttg taatacatgc gcacatcttc ccaataatgg
    17341 tttaatttct gtgcattttc aatcgtgatt tctggcagcc gtggaccatt gaccagtgca
    17401 taatacaagc tgctcatgct aggctgacta gtattcccac tcattgcact cattgccacg
    17461 tcaacgatgt ctactcctgc ttttgaagca gcagaataag tgatgattcc attcccgctg
    17521 gtatcatgag tatgcagatg gattggcaga tctgtcgttt ctttcaattc actaatcaaa
    17581 cgataagctg cctgcggttt aagcagccca gccatatctt tgattgcaat gatatgcgca
    17641 cccagttgtt ctagttcctt tgccatgtct ttataatact gaacattgta ttttgcccga
    17701 cttggatcgt tgatatcgcc agtatagcaa atagctgcct ctgcgatttt accagtatcc
    17761 cgtacagctt ggatactttt ttccatttgt ggagtccagt tcaaactatc aaagattcga
    17821 aatacatcga ttccttgcgc tgctgcttct ttgacaaatt ccaccaatac attgtcagga
    17881 taattagaat acccaacggc atttgaacct ctgaaaagca tttgcagcaa cgtattaggc
    17941 attaaagaac gaatttttct caaccgttgc caaggatctt cattcaaaaa gcggtaagcc
    18001 acatcaaatg ttgctccccc ccacatctca cttgaaaaga gttcaggaag accttctcca
    18061 gtcaaacgtg caatctgctt gaaatccttt gtccgaacac gagttgctag cagactttga
    18121 tgggcgtctc taaatgtcgt atccgtcaac aacaaatttt cctgtccttt gatccattcg
    18181 accaaagcat cggcaccctt cgcatctaat acattcttag ccgttacgta atcagttctt
    18241 gtgattagat ctttaggcat acgtggttct tcataaaatg gtttttcgcc actttcgata
    18301 cctggaaaac cattcacagt aatttcaccg atatatttca tcgttttgtt tccgcggtct
    18361 cgtagtcgag gaaattcaaa taatgttgct gtactatcaa tgaaggttgt ttttgcattt
    18421 cctgattgga attcaggatg tgtgatgaca ttgagcataa atggaatgtt tgttttcact
    18481 ccacggattc taaattcttt caagcaacgt tccattttct gaatggctgt ttcaaaggta
    18541 gcaccatgcg tacatacttt tactagcaat gaatcaaaat acggtgtcac tacatagccc
    18601 gcgtaagcat ttccgacatc cagacgtacg ccaaaaccac caggtgaacg atacgtatcg
    18661 attttccctg tgtctggtaa aaagttattc aacgggtctt ctgtagtgat tcggcattga
    18721 atggctgaac catttaattt aattccttct tgctgaggca atccgatttc tttatgcaga
    18781 tccaaacctt gagcaatcaa cagttgcgtt gttacgatat caatatctgt aatcatttct
    18841 gtgatcgtat gttccacttg gacgcgaggg ttgacttcga taaagtaaaa atcatctcct
    18901 tcaacaagaa attctactgt acctgcgttg acatacccga catatttcat caattggaca
    18961 gctgcctggc agattttttg acgttgctgt tcattcatag agacacaagg cgctacttct
    19021 actacttttt ggtgacggcg ctgaacagaa caatctcttt caaaaagatg gatgacgttt
    19081 ccatgcgtat ccccgagaat ctgtacttcg atatgcttag gattagcgat gtatttttca
    19141 acatacactt catcactacc aaaggctgct tttgcttcgc ttttcgcacg ttcgtagccc
    19201 tctcttgcac tcttttcatc gtgagctaca cgcatgccgc gtccgccgcc gcctagagct
    19261 gccttgatca taatcggata accgtattgt ttcgcaaact ccaaggcatc gtctatagaa
    19321 gcgattggtc catctgtgcc tggaatcgaa gcaatccccg cttcgatagc tgcagctttt
    19381 gctttgattt tatctccaaa aatatctaaa tgatgcagtt tgggtccaac aaaaatgatg
    19441 ccttcttctt cgcaccgttg cgcaaaattc aagttttccg acaaaagacc gtaacccgga
    19501 tgaatagcgt cagctccgca ttctttcgcg atagaaatga tcccttcaat atctagatac
    19561 gcatcaatcg gtttttttcc ttgtcctact aaatatgctt catccgcttt gaaacggtgt
    19621 acggaatatt catcttcttc tgcatagatt ccaacagttt taattcctaa ttctgtacaa
    19681 gcacgaaata cacgtacagc gatttcaccc ctgttagcaa ctaaaacttt tttcatcgaa
    19741 ccactcctac atcttttttt attataaacc taagctcttg cgtttttcat ccgcactgat
    19801 gttgaggaca aagccgacac aaatcgaaag catcagcaaa ctggagcctc cttgacttaa
    19861 aaatgggaac gtgatccctg tcaaaggaat aacgccagta attcctccaa gattgacaaa
    19921 tacttggatc aaaaacattg cgccgatccc aatgcacaaa agcgaattga acggatcttt
    19981 tgaacggata cctacaagga caatgcgcac aatcataaag aatagcaacg ctaatatcaa
    20041 aatccccata atcactccca gttcttccac tacgattgca taaataaaat cggtttgtgc
    20101 ttcttgtaaa aatccttttt tctggatact gttacctaat cccctgccaa atagtcctcc
    20161 gttgaacatg gcatagtagc cgttgactag ttgatgccct ttatttaatt cgtctgagaa
    20221 aggattttgg taaatagcaa atcgattata gatatactgt aaacgtccgg gtaagatctt
    20281 tccgtttgtg atattgatca gccagatcgt aaaagtactt agacaaaagc ctcctactcc
    20341 tacaatcaaa gtatacacat aattgactcc gctggctaaa agcagaacca aaataatcaa
    20401 aaagataact gccgcattac caaaatcggg aagaatagcg actaatgcag taagtccgat
    20461 aaccattgca agtggacgtt tcacagtccc aagaaaatct ttttgtacac tgttttgtct
    20521 tttagataaa gtcagagaca ggtaccagat agaaatgatt tttaggtatt cggcaggttg
    20581 gatcgagaat cctgcaattt gcagccaacc tttcgcccca ttgatctcct taccaaaaaa
    20641 cacaacaatc agcagcaaaa tcgtcaaaac tgcgatggct gccatgatca aacgctggtt
    20701 tttcaaaaca tctgttttca ttttataaag tagtgcgata gcaatcaagc taagcaccca
    20761 aaaaatactc tggttaatga ctgatgcgga cggattttgt ccgttttcta ataatagata
    20821 cgacgtagaa ctatatacca tgatcaatcc caatacacat aaaattaaat agggaatcaa
    20881 gatgctatag tctaatagat gccttttttt cactttctta ggcaagtcta ttcctccttg
    20941 ccaacttctt ttttatcata aatttcttgg taaacctcac tatataactg attcaattct
    21001 cgttctagct cacttaacat cagctggccc tcgttctttc cgattactcc tatacggaca
    21061 gcaaaaccga cttgccggga aaagccgtac atctgtgtat ctaccacttc ttcgaaggct
    21121 ttgcattgtg agatacagag actgttgttt tgatttcgga ttaatcgttt gatccgttct
    21181 gcttcatcat ttaatacttg gatcgcaagt tttttcgaca aagaatccat ctgtgcttac
    21241 ctcccattct aattccttaa ttataacata tttgattttc ataaggagca aaaaaaacag
    21301 atttaaccag ttgtttttat tcatttttac gtattcgttc tttctactta tccagaacgt
    21361 tagagtccgc attttttatc cgagataaaa aggcttcgcc atatttatca agcttgcttt
    21421 ggccaactcc ttttatctga agcatctcca atctgcttga tggacgtatt cggctcattt
    21481 ctttcaatgt ggaatctgaa aatacgacat acgggggaac gcctgcttct gatgctagat
    21541 ccgttcgcag ttcccgcagc acttcaaata gtgtatcggt ttcttcatcc gatagctgct
    21601 ggactttctt gggttccttt cgataaacag attcttgtcc tttcaatact tgtatccctc
    21661 tttctgttac tttcaaaaca ggatactcac cattgatggt cagaagatag ccattcgata
    21721 tcaaatattc aattaactgc atcgtttctt tttgcgattg gtttttcatc agtccatagg
    21781 tagataaatg tccaaaacct aaagcttgca gcttttgttc tttcgatcct gctaagactt
    21841 tcatcagcat ttgtttacca tagttctcac ccatacgttt taagcaagaa agaaccatct
    21901 gtgcttctgt cgttacttct actatttctc tgtcatctaa acagttacca caacggttgc
    21961 acggactgct tgtctcgcca aaatagttta ggatgtattg ctgaagacag gactcaatat
    22021 acacgtattc tgtcatcgct tttaattttt catattcttt ttgcttttgc ccttcttctc
    22081 tttgggactg ctggataaaa aacttttgga cttggatatc ttgaggagag aaaagcagga
    22141 tggcttcact ggaaagacca tctcttcccg cccgaccagc ttcctgatag tatgcttcta
    22201 gagagccagg aatttgataa tggataacaa aacggacatt gcttttattt atacccatac
    22261 caaatgcatt tgtcgctaca atcagctgca agcgatcgta taaaaaggct tcttgcattt
    22321 ctgttcgctc attctcattt agaccaccat gatatctacc cacggaaaaa tcgaattttt
    22381 taagcaaatg atagagacgg tctacttctt ttctcgttgc tgcataaatg ataccagatt
    22441 gatttttgtt gatctttaaa tattcgatta gatattgttc ttttttttga tctttaatta
    22501 cttgaaatcg tagattttct cgttcaaaac cagtctgaat atggttgttt tctggaattt
    22561 ttaaaagtct ttttatgtca gcagctactt gcacagtcgc tgtagctgtc aaagcaacaa
    22621 ttacaggacg atttggcagt tgatctagct ggttcgccat ctgtaaataa cttgggcgga
    22681 aatcatgccc ccactgagag atacaatgtg cctcgtcaat agcgatcata cggacgttcc
    22741 atcgcttcat tgcttggata aaatccggca tgataaaacg ttcaggagcg atataaagta
    22801 aacggtactg ctgtctatcg atttcttgga agcgtctagc tgtttcgtaa ccatctaacg
    22861 agctattgat aaaagtggcg ggtataccta attgattcgc cgcgtccacc tgatccttca
    22921 tcaaagagat taaaggggac acaaccacag taagcccatc taatagtaaa gctggaagct
    22981 ggtaacagat tgatttccca cttccggtag gcatgattcc taatacattt tcctgatcaa
    23041 tgactttctg gataatttct ttttgaccag gtctaaactc atcatagcca aagtattgtt
    23101 ttaataagtc gttcatcttc ccgctccttt ctcttgccat tttacttcga ttttcttcga
    23161 aggacaagtt gatctccttt cgtgaaacat tatcttatta tgaaacaaaa actctctaga
    23221 aataagtttt ttaaaagtct tttaaacgtc tttcggcgta ttttctgaat ttatgtccat
    23281 tgacaaagat gacttctcca cagaaaagag ttggacaaaa aaagaaacca gctatttttt
    23341 ataacaagct ggtttctctc ttcatccttt tacagattat tttttc
//
LOCUS       DAZUKA010000031        23354 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000031 DAZUKA010000000
VERSION     DAZUKA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.031"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..910)
                     /gene="deoD"
                     /locus_tag="ACG8PN_000962"
     CDS             complement(203..910)
                     /gene="deoD"
                     /locus_tag="ACG8PN_000962"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4861137.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(927..1745)
                     /locus_tag="ACG8PN_000963"
     CDS             complement(927..1745)
                     /locus_tag="ACG8PN_000963"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4861138.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1769..2935)
                     /gene="deoB"
                     /locus_tag="ACG8PN_000964"
     CDS             complement(1769..2935)
                     /gene="deoB"
                     /locus_tag="ACG8PN_000964"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4861139.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3034..3990)
                     /locus_tag="ACG8PN_000965"
     CDS             complement(3034..3990)
                     /locus_tag="ACG8PN_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861140.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3990..5123)
                     /locus_tag="ACG8PN_000966"
     CDS             complement(3990..5123)
                     /locus_tag="ACG8PN_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861141.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5116..6678)
                     /locus_tag="ACG8PN_000967"
     CDS             complement(5116..6678)
                     /locus_tag="ACG8PN_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861142.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6890..7972)
                     /locus_tag="ACG8PN_000968"
     CDS             complement(6890..7972)
                     /locus_tag="ACG8PN_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4861143.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8038..8436)
                     /locus_tag="ACG8PN_000969"
     CDS             complement(8038..8436)
                     /locus_tag="ACG8PN_000969"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4861144.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8457..9119)
                     /gene="deoC"
                     /locus_tag="ACG8PN_000970"
     CDS             complement(8457..9119)
                     /gene="deoC"
                     /locus_tag="ACG8PN_000970"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4861145.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9165..10466)
                     /locus_tag="ACG8PN_000971"
     CDS             complement(9165..10466)
                     /locus_tag="ACG8PN_000971"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4861146.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10607..11623)
                     /locus_tag="ACG8PN_000972"
     CDS             complement(10607..11623)
                     /locus_tag="ACG8PN_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861147.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11931..12536)
                     /locus_tag="ACG8PN_000973"
     CDS             complement(11931..12536)
                     /locus_tag="ACG8PN_000973"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4861148.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12732..13655
                     /gene="coaA"
                     /locus_tag="ACG8PN_000974"
     CDS             12732..13655
                     /gene="coaA"
                     /locus_tag="ACG8PN_000974"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4861149.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13714..15279
                     /gene="guaA"
                     /locus_tag="ACG8PN_000975"
     CDS             13714..15279
                     /gene="guaA"
                     /locus_tag="ACG8PN_000975"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4861150.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15770..17401)
                     /locus_tag="ACG8PN_000976"
     CDS             complement(15770..17401)
                     /locus_tag="ACG8PN_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4861151.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17415..19088)
                     /locus_tag="ACG8PN_000977"
     CDS             complement(17415..19088)
                     /locus_tag="ACG8PN_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4861152.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19101..19418)
                     /locus_tag="ACG8PN_000978"
     CDS             complement(19101..19418)
                     /locus_tag="ACG8PN_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4861153.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19443..20843)
                     /gene="lacG"
                     /locus_tag="ACG8PN_000979"
     CDS             complement(19443..20843)
                     /gene="lacG"
                     /locus_tag="ACG8PN_000979"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4861154.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21080..21865
                     /locus_tag="ACG8PN_000980"
     CDS             21080..21865
                     /locus_tag="ACG8PN_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4861155.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21868..22560
                     /locus_tag="ACG8PN_000981"
     CDS             21868..22560
                     /locus_tag="ACG8PN_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4861156.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22834..23205)
                     /locus_tag="ACG8PN_000982"
     CDS             complement(22834..23205)
                     /locus_tag="ACG8PN_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4861157.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         6717 a         5225 c         4217 g         7195 t
ORIGIN      
        1 aatattcatc aaacatgaaa agctgttttt tgaaattgtt ccttgtgact cagagttaaa
       61 cggcagattc acaacctctt ggtaaacggt gttcaaaagc ttacatttgc aataataagt
      121 ccagccaagt caaaaacatg aggagctatt tttgcttgga tgttcttatt acttaatgca
      181 gggcagcttt ttcatttcct tcttattctg ctatcgctgt ttccaatcct acttcgatca
      241 tttcattgaa cgtagattga cgttctgcag ctgttgtttc ttcaccggtg atcaaatggt
      301 cgctaacggt catcaaactt aatgtctgca catggtattt cgcgcctaag taataaagag
      361 ccgctgcttc catttccact cctaaaacgc caagttcagc taattggaac gtctctgtca
      421 aatcatcttt gtagaaagaa tcttccgaaa gaacatttcc tacatgaacc gtatagccct
      481 tttctttagc tatctcatat gctttcagta atagattgaa atcactgatt ggaggaaaat
      541 gaaaggcttg gaaatttttt tcgatcatcg aagaactggt cgctgcacct tgtgctagta
      601 ctaaatcacg aacatggacg tcttttgaca atgcaccgca tgtacctact cgaatcaatt
      661 tctttacacc atacgactgg atcaattcat gggcatagat ggtggctgat ggcattccca
      721 ttcctgttcc ttgaacagaa atacgttctc ctttatattt gccggtaaag cctaacatac
      781 cgcgtacctg attgtaacaa actggatctt ccaaaaaagt ctccgcaata tatttagctc
      841 gcaaaggatc tcccggaagt aaaatcttat cggcgatctc gccttctttc gcttcaatat
      901 gcacactcat tttattgttc ctcctattac aataatgtca atgtttcttt aaccaatgct
      961 ttaaaagatt gtttgactct ctgagttgtt tctacaactt cttcatggct cagattcgct
     1021 tgcatacctg ctgctaagtt ggtaatacaa gagattccta acacttttaa tccgctgtga
     1081 gctgctacga tgacttctgg aacagtcgac atccctactg catcggctcc tattgtccga
     1141 gacattcgga tctccgcagg cgtttcataa gttggtccag aatagcccat atatacgcct
     1201 tctttaagat ccaaacctag tttttctgct gctgcttttg ctgttgcttg atagtcaggc
     1261 gtataagctt gactcatatc tggaaatcta gggcccattg catcttcatt cggaccgatc
     1321 aaaggatttt caccagtaaa attgatatga tcggtaatca gcatcaaatc tcctggtaca
     1381 aatgattcgt tgacaccacc acatgcatta gtcacaatga ctgaatgggc ccctagtccg
     1441 gccatcacac gtactggata ggtcactgtt tgcatggaat gaccttcata gtagtggaat
     1501 ctcccttgca tggctaacac tttcttgcct gccagagacc cataaactag ctttcctgca
     1561 tgcccaacta ctgtcgaaac aggaaagaac gggatctcat cataagaaat cattacagca
     1621 tcttcgattt catctgctaa ttcacctaat cctgaaccta gaatcaaacc aaaatcaatt
     1681 tcttttactc ctttatcaag gataaactgg gtcgtttctt gtaacatctc gcttaacttg
     1741 gtcatttgct ttctcctatt taaaaagatt attttaataa tttcaagaag ctttcgccat
     1801 tttcagtcga agaaacacta aagttctcag caatcgttgc tgaaatatct gaataatatc
     1861 cttgaggcaa tgccccttgt tctttcattt ttttactgta aaccaataac ggtacatatt
     1921 ctcgcgtatg atcggtacca gtaaacgtcg gatcgtttcc gtgatcggct gtaatcatta
     1981 gcaagtcgtc ttcttccatc gcttcaagca attctggaat tcgtaagtca aattcttcaa
     2041 tcgcatgcgc atagccgact acatcccgac gatgaccgta taatgcatca aaatctacta
     2101 aattggtaaa gctgattcct gaaaaatcgt ttttcataac tttcagcagc tggtcaacgc
     2161 catccatatt gcttttcgtg cggacagatt cagtgattcc ttgtccgttg aaaatatcgt
     2221 tgatctttcc aacagcaatc acgtcctttc ctgcttcttt caaagaatca aggactgtgt
     2281 gtccgaatgg attcaacgca taatcatggc gattgcttgt tcgtgtgaag tctcctggtt
     2341 cacccacgta cggacgtgca atgattcggc caatcatata cggatcatct tttgttatat
     2401 cacgaacgaa ttggcaaatc cgatataatt cttctaaagg aatgatttct tcatgtgccg
     2461 cgatctgaag gacaggatcg gcagaagtat atacaattaa atcgcctgtt ttcatttggt
     2521 gttcaccgta atcgtcaata acagctgttc cgctataagg tttgttacat acgactttac
     2581 ggccagaaaa ttcttcgatt tgtttgatca gatcatcagg gaatccatta ggaaacacac
     2641 gaaatggagt tttgatattc aatcccatga tttcccagtg gcctgtcatc gtatctttcc
     2701 ctacagaaac ttcttctaat ttcgtcgcat agccttgatg atctttcact gattccacac
     2761 cttcaagagg acggatcgtt cctaaaccaa gctgctctaa atgtggaacg ttcaatcctg
     2821 cttctttcgc aatgtgtcct aatgtatcag aaccaacatc accgaactta tcggcatctg
     2881 gtgcttctcc aattcctact gaatccatga cgatcagatg tacgcgttta aacatgatta
     2941 ttccttcttt ctttttattt ttattgcctt taaaacaaaa agggcgagac gaaaaacact
     3001 tcccgtaacc cacccttttt attcgctaaa agattatttt gatttgatat agttcttccc
     3061 gtttgctttt ggaccagaag ctttccctaa gaaaacaacc agcacaatga ttgtcagcaa
     3121 atatggagca atttgcagca atactggcgg aatcgaagaa ataactggta attgtgcgcc
     3181 aatgatactc aagctttgag caaaaccaaa gaacaatgag gcacccattg cccctaaagg
     3241 attccactta ccaaagatca tagcagccat ggagatgaac ccttgtccaa cgatcgtacc
     3301 agctgaaaag ttaccggaaa tcgactgagc aaaaacagca ccgcccatac cgcctaacaa
     3361 gccagaaatc agtacgcctg aataacgcat agcatataca ttgataccta atgtgtcggc
     3421 agcttgagga ttttcaccaa ctgaacggag gcgcaagccg aatttcgtct tgaagatgat
     3481 cacccaagct aggacagcaa tcaaaatagc acaataagca ggtaagaatg tatttttaaa
     3541 gaaaattggt ccaatgaccg gaatatctgc taatcctggg aaagaaaaat accccagatt
     3601 ctgcgtgatg ttatcagttt gacctttacc ataccatgct ttgatcaaga aaacacctaa
     3661 agcaggtgcc atcagattaa ttactgtccc gctgataata tggtcagcac gcaaactgac
     3721 tgttgctact gcgtgaatca gagaaaatag gatacctacg acgccaccca ctaaaatagc
     3781 gatccatggt gtcatagaac caagtgatgg actcatcgtc aagttgaaaa cgatggaact
     3841 aaaagcaccc atgaccatga ttccctctag ccctacgttt acgatcccgc ttcgttcaga
     3901 aaaagttccg cctagtgacg ttagaattag cggtgttgaa taaaccagtg tagaagtaag
     3961 gatcaaggca attaattcga tagtcgacat tatagttcgc ccccttcatg gttttgattc
     4021 gttggtttcg attctattgt cgcaacgatc tcttcttgtt ttttcccttg gaagacttta
     4081 gccatgacga aacgaatcag gaagttgatc ccgacaaaga agataatcaa agcaatcgat
     4141 acattgacga tctcaaacgg aacacctgct cctgtctgca tgccaagtcc accgatcttc
     4201 aaaatactga acaagatcgc tgataaaaga ataccgattg ctgatccaga accaagcaag
     4261 gaaacagcca ttccgtcaaa tccaatagat agcgaagtcg tttgaacaaa gaagttttga
     4321 tacgttccta atccttcaac cacaccgcca agccctgcca aagcgccaga aatgaccatc
     4381 gacaaaacga tcgtacgttt actactcatt cctgcatact cagaagcaaa aggattcaat
     4441 ccaacagaac ggatttcaaa tcctaaagtc gttttcttca tcagcagcca aacgaaaata
     4501 agcgcgatga ttgctaaaaa taatcctgag tttacgcgag agccgccaaa agcttgtgtt
     4561 aaccatggta accgcagtga ggcattttga ctgatcatat tggaagagtc caaacttgag
     4621 cgatagcttt caggcatcac ttcatgtaaa gcaaatgtgc ttacgtataa taagatatag
     4681 ttcatcatga ttgttacgat cacttcactt gttccaaaaa atgctcgaag taaacctgga
     4741 acagccgctg ccatcgcacc agctaaggca cctaagatca cgcataacgg aagcaaaaca
     4801 acttttggca gatctggaaa cgcaagtgcc acccagacac tgacgatcca cccgcaaagt
     4861 gcttgtccag aaagcccaat attgaaaaac ccagctgagt tagctacaga aaaacctaat
     4921 gctgtaaaaa tcaacggcgt tgcttgcctt aacgtttcac ctatccctct ttgcgaacca
     4981 agggctgcat ctagcataga agaataacct tggatgggct gaaaaccaaa agcagccatc
     5041 aatatcgcgc ctaagatcaa tcctaaaaca actgaaagta caggcactaa taaattacgg
     5101 aatttttcgc tgcgatcatt catgtgcgcc agcccccttt tctaactctt ttcgtgcttc
     5161 ttctaaggta tagcccgcca tcaatagacc taattcattt tcagatgttt ctttcggatc
     5221 aacgataccg acaatttctc cttcatggat aacagcaata cgatccgaaa catttaaaat
     5281 ttcttctaat tcgaaactga tcagtaacac tgctttgtat ttgtcccttt gttcgatcag
     5341 acgtttatga ataaattcga tcgcacctac atccagtcca cgagttggat ttgcaacaat
     5401 caacagatca ggatctcggt cgacttcacg agcgataatt gctttttgct gatttccgcc
     5461 tgataaagct tttgcgggaa caagttcatt cgttgttcga acatcatatt cttcgatcaa
     5521 ttctctggca tgttcattca ttactgaata attcagcatg ccgtttttac tgtaaggttt
     5581 ttgatgatag gtttgtagtg cgatattttc agataatgtc atatctagga ctaacccgta
     5641 tttatgacgg tcttctggca cgtgtcctac accatgttca gtgatttttc ggggtttttt
     5701 attagtgatg tcttcccctt ttagcttgat atgtccgctt tctgcctttc gcaaaccagt
     5761 caaagcttgg atcaactccg actgcccgtt tccatcgatt ccagcgatac caagtacttc
     5821 gccagcacga acctctaagt tcaagttttt cacggcttct aatccacgat tttctttcac
     5881 cactagattt tcaatagaca aaacgacttc ttgaggcttc gcttcttttt tcatcgtttt
     5941 gaatgaaacc gttcttccaa ccatcatatc ggctaattgt tgtgaggtta cgtctttaac
     6001 gttgactgta ccgattcctt ttccacggcg gataactgta cagcggtcag ctactgcttt
     6061 gatttcatct aacttatgcg taatcaaaat gatcgacttg ccttctttga ctaattcctt
     6121 catgatcacg attaattcat caatttcttg aggggtcaat acagctgtcg gctcatcaaa
     6181 aatcagtaca tctgctcctc ggtaaagtgt ttttaaaatt tctacccgtt gttccattcc
     6241 aactgaaata tcacgaacgt aagcatctgg gttgactgac aatccatact gttcagaaac
     6301 tttttggatc tcttttcgtg cttttttatg gtcaagcatc cctgcgcgac taggctcgct
     6361 tcccaacacg atgttttctg ttacagtaaa ggcatcaaca agcataaagt gctgatggac
     6421 catcccaatc cctaattggt tggctttggt cggacttgtg atctttactt ctttcccacg
     6481 catcaaaatt ttccctgatg tcggctctaa taatccggaa agcacgttca tcaatgtaga
     6541 tttgccagca ccgttttctc caagcaacgc atgaatttct cctgccttta cttgtaaatt
     6601 gatgttgtca tttgccttga atgttccaaa ttctttggta atattctgca tttctatgac
     6661 aatattttct tgaaccacct ttttttcaca tcctaacgat ttctatctag ggcaacgatt
     6721 gactaagatt cacgcttagt gagcgttctt cttttgaaac aaacattact tcagggagtt
     6781 gttcccttat ccaaactgtc acaaggcagc ttggataaat gaacaattct tgttctaatc
     6841 aaaccatagc cttgtcaaac atgtgtctaa caaggctata gaaatggttt tagttttctt
     6901 caggagtttc tggaactttt acgtcacctg aaatgatttt ttcttttgct tcatcaactg
     6961 cttttttcgc ttcgtcagaa agttgtcctt ctgttagacc aacaccgtct tctttcaatc
     7021 cataaacagt gtgttcgccg ccagggaatt taccgtccgc tgatttttgt gccaaatctt
     7081 ctactactgt tccaactgct ttcagtgtag atgtcaatgt gaagtttttc ttttcgccgt
     7141 ttaatgtgta ttcgccttcg tctgattggt cgcggtcaac accgataacc caaacttttt
     7201 tgtcgcctga ttcgttcaat gattttgctt cttggaatac accgttacct gtaccgcctg
     7261 aagcatggaa gatgatatct gcgttttgtg catacatacc ttgagcaatt gaacgtcctt
     7321 tatctggagc agagaagtca ccagcatatt gattcaatac tttgatttct tttcctaatt
     7381 ctttcgcacc tgcatcaaca ccggctttga atccagcatc aaaacgatcg attacttcac
     7441 cttttacacc gccgataaag ccgactacgt ttgtttcagt cgtataagct gctgcaacac
     7501 ctgcaagata agaagcttcg ttgtctttga aagttgcaga tacaacgtta tctaatccat
     7561 cgatgacatc atcgataata acaaagttcg tacctgtatt gtttgttgct tgttcttgga
     7621 tcgctggttt tagtttataa ccaataccaa aaatcgtttt gaatccagca ttcaatgctt
     7681 ggtcaatatt tgggatgtaa tctgattcat ttgaagattg gaaatattgg aagccatcat
     7741 ttcctcgtga aagtccttga tctttccccc atttttctag tccttcccaa gctgactggt
     7801 tgaatgaacg gtcgtctaca ccacctgtgt ctgtgatcaa tgctgcagtt gttgtgtctg
     7861 atgagctttt gccaccggat gaatcagtag agccgttgtt gccgcatgca cctaaaaata
     7921 accctgctgc tagtgcgaca gcacctaaac caaataattt tgcttttttc atttctgtaa
     7981 agcctcctaa aataattgtt ttatttttca acagtcagct gactgaatga acatgagtta
     8041 tagatctttt tcagtaaatg cataaggcag cagtgcacga acggtcgttg ccttcgtcac
     8101 accattgtct cctactagcg taaccggcat atcaggggaa cagaattctg ccattacttg
     8161 tcgacatgca ccacaaggag agataggatc gggggtgtgc cccgctacga ctagatgcgt
     8221 aaatgttttt tctccttcag atactgcttt gaaaaaagct gtgcgctcgg cacaattcgt
     8281 taatccaaat gatgcattct caatgttgat gccttgataa atcttgcctg attccgttac
     8341 gagacaagcg cctaccggaa aatgggaata aggaacatag gctttatcta acgcttcaat
     8401 agctgtgttg atccattctt gtttgagttc tgccatgagc ttctcctttg aaaaaattaa
     8461 tagccttcac cagttgaacc agatacgatt gctactcctg cgcttgttcc gattcttgta
     8521 gcaccagctt cgatcattgc cattgcttct tcttcgttat ggataccgcc ggaagctttg
     8581 acacccattt ctggtcctac agtttcacgc atcaaacgga catcctctac ctttgctccg
     8641 cctgttgaga aaccagttga tgttttgaca aaatcagcgc ctgcagcttt tgctatttcg
     8701 caagctcgga tgatttcttc tctatctaat aacgatgttt caataataac tttgacaagc
     8761 gcacgatctt ttgctgcttc aacaactgct tcgatatctt tttggacttg ttgatcatcc
     8821 tttgatttca aagcaccgat attgatgacc atatcgactt catctgcacc gttattgatg
     8881 gcatctgttg tttcaaatgc ttttgtctcg ggtgtgtttg ctcctagagg aaacccgatt
     8941 actgtacata cggctactgg ctcaccttgt aattgttctt tcgctagcgc aacccatgct
     9001 ggattgatgc agacagaata aaaatggtat ttttttgctt cttcgataat ttgcatcaca
     9061 gcttctttag atgcgtctgc tttcaaaata gtatggtcga tcattcggtt tagttccatt
     9121 tcttttcccc ttttctttaa aacggtaaag tccgcttttt tcctttattc tgtgatgatg
     9181 tcatgaatca acgttggctt cattgccgat tcaccaattt caatattttt atataataat
     9241 tcaatgacag aagtgatttc ttcgtcgtta ctatagatcg tcaatagtga ctcacctttt
     9301 gtcacagtgt caccaatttt tttatgcagt ttcaatccaa cagcgtgatc aatatcttct
     9361 tctttggttt tacgcccagc acctagcatc attgcagcaa ttcctaattc atttgccacg
     9421 atttttgaca caactccgct ttgtttagca ggaagctcca ttgtgtagcg tgcagtcaaa
     9481 attcgttctg gatgttccac gatcgctggg tctccccctt gattttggat catttctttg
     9541 aattttgcta acgctttacc tgattccaga gcttcttgaa gcaacgttct tgcttcatcg
     9601 gttgtcttag ctttgccagc aagaacaacc atttggcttc ctaaggcgta gcacatttct
     9661 actagatctt ctggaccatt tccttgtaat gtttcgattg cctcgacaac ttctagacta
     9721 tttccgatag cttcgccaag aggttctgac atatctgaga taaccgccac tgtttctcgg
     9781 ttagccagtt tcccaatgct cgtcatggtc tttgccagac gtcttgcatc ttcgatgttc
     9841 ttcatgaaag caccgtctcc agtggtcaca tccaaaacga ttgcatctgc tcctgccgcg
     9901 atttttttac tcataatcga actggcaatc agcggaagag aatcaacggt cgctgtgacg
     9961 tcacgcaagg catacaattt tttgtccgcc ggtgccaagt ttcctgattg accaattacc
    10021 gctactttac tttcattaac aatgcgtaca aattcttcat ccgaaagctc gatctgaaat
    10081 ccgggaattg cttctagctt gtctagtgta ccacctgtgt aacctagacc gcgtccagac
    10141 attttagcca ctgtcactcc gacactagct actagggggg ctaaaatcaa tgtggtcgtg
    10201 tcaccaacgc cacctgtcga atgcttatct acctttattc cttcaagaga actcaaatcg
    10261 atgacatcac cagaattagc aatcgccaat gttaagttcg tgatttcttc atttgtcatg
    10321 tcttgataaa aaattgccat caataatgca ctgacttgat aatctggaat ctttccattc
    10381 gtatagttcg tgacgatgta ctcgatttcc tctttcgaca gctcgtttcc atccctcttt
    10441 ttttcaatca gatccaccat tcgcattgcc tgatcctcct tgtcttcatt cggtattata
    10501 cattattttt aggaaaggta aaccacttta gtaaactggt ttactaaaaa taaaaaaagc
    10561 actaactagc ctttgtaaat gcttacataa gcaatatacc gccgagttag ccctttgtca
    10621 atacttttac gctctcacga ataattaatt ttgtttcgaa cactttgttc ggaacttttc
    10681 tttgaggaaa ttcgatcgta tcgatcagga aacgtgcagc ataaaatcca atatcaaaac
    10741 ttggctgctg cactgtcgtc aattgcggaa tcatgtattc tgacagcttt aatccatcga
    10801 atcctatgac tgagatatct tctgggattt ttttgcccaa ttcatcgatc gcctgatagc
    10861 atccgatcgc catggcatca ttcccgcaaa aaatagccgt gatatcttta tgtttgagta
    10921 atttttttga tgcagtatat ccaccttgga cagtcaactc tccatgttca acaaatttcc
    10981 cattaaaagg caggccatga tctttcagcg catgacgata tccgttgaaa cgttcttcta
    11041 attgatagta tccagtggtt tccttcagca tgccgatctt cgtatggcca ttttcaataa
    11101 acagcgaaac agcttgatag gcgccctcat attcctttac gataagccgt ccgttatccc
    11161 gacgattcaa tccgcgatcg attaaaatga ttggcatttt ttgataaaaa ccgctgtcta
    11221 atgcatattt ctcaggtaaa atatttggtg tggccagcaa gattccgtcc actgagcggt
    11281 gggaaagctc ttgtaaatac ttgatctcat tttcttggct atgacgagaa ttgcataaca
    11341 tgatcacata gcccaaagga ttcaaatagc tctctacacc ttcgatcagt ttggagaaga
    11401 agaaatcagt gacatcaggg acgatcattc cgatggtctt tgaatgtcga ttgatcacat
    11461 ttgaagcgaa ataatcgggc ttataatgat gttcattcac gatcgacaac actttttttc
    11521 ttgtcttatc actaaatctg cttcctttgt tattcaaaat ctgtgagaca gttgttactg
    11581 atacgcctgc catttctgcg atatctctga tggtcagctt catcctattg gctcccttct
    11641 atctaaattc cctatcagtg taccaaaacc cccaagaaaa gaatagagaa aaaaggaaag
    11701 tatgtttttt gataaaaacc tcctttatct accgacttgg cagccttcta tacaagttct
    11761 gtatcttatt tattttatcg taaaaaacaa cgactctgtt cctactattt ctttttgaag
    11821 aaaaaatagt tctttgtttt taaaaacttc tcaacaaaaa aagaactgat acgtacccgt
    11881 acatgcacgc ccagttccac tctttcgtcg ctccactatt tttcagctag ttattctttc
    11941 acacttttga tgatataata tcctttatct tttgtgacga tttctgcatt accaaatgtt
    12001 tcttccattt tcttttgcgc actcggcgct ccttgtttct tctggatcac aattgtcagt
    12061 gtaccgccct tcttcaaccg aggatatgcg cctgtcaaaa tttcatgtac aacttttttt
    12121 ccagcacgga tcggcggatt gcttacgata gctgcatagg tttcttcatg aactgcttca
    12181 tagatattcg attgatggat atcaacatgt tcgatttggt tacgctttgc gtttccttgc
    12241 gctagactta ccgctcgttc attaatgtcg accatttcaa caaaccgctg tgtcgcaaat
    12301 gccaaagcta acccaatcgg tccatacccg catcccacat ctaagatctt tccttcatca
    12361 ggtaactctt cccaatcgaa agcatcaatc aggacacgtg aaccataatc cactgtttca
    12421 cgtgaaaata caccagaatc tgttacaaat tgaaaggttc tgccttttaa ctcaaaagcc
    12481 cattgttcaa aatcgtgcgc cgtatctggg cgttcagaat agtaatgatt tgtcatgtct
    12541 tccatccttc tcatttcttt catgacaaca gtctatcctt ttatgtatcc gtgcgcaagt
    12601 caagaataag actccgtttc tttctattat aaagattata caaaaagatc ggcaatctct
    12661 cttaagtggt ggggtttgcg tttattttgt gttaaaatag ttccattgat gggagaacgc
    12721 aggaggctaa tatgaatgat ccaatgaact actaccgtgt ggcaagagaa gaatggcgag
    12781 aattttatcg taatgggcaa gcacctttga cacaagatga actagataat atcaaaagtt
    12841 tgaatgaccg catttccatg caggatgttc gagatatcta tgtacctctt gctcatctga
    12901 tttatcttta tatgaaagaa tttgaatctt tgttcttgag taaaggttta tttcttcata
    12961 aatacgtaga agtaccgcct tttattatcg ggatcgccgg aagtgtagcc gttggaaaaa
    13021 gtacaacagc cagactgctg cagacgattt tgtctcgtac cttcaaacga agaaacgttc
    13081 agctgatcac gactgacgga tttctttatc caaatcgtat attaaaagag cgtggaatca
    13141 tggaccgtaa aggatttcct gaaagttatg atatggagat gctgatcgat ttcttgaacc
    13201 gtgtaaaatc tggtcaggaa gagataaaag tacctgttta ctcacatgat gtgtatgata
    13261 tcattccaga caagtacgaa ttgatccagc agcctgatat cttgatcgtt gaggggatca
    13321 atacgcttca attacctgcg aatcagcaaa tctacgtcag cgactttttt gatttttctg
    13381 tctatgtcga tgccgatccc gtgctgatcg aaaaatggta tttggatcgt tttggtgcgt
    13441 tactggatac ggctttccaa aatccagaga attattatta tcgttttgct atcggagatc
    13501 gtgaagaagc ctttaaaatg gcaaaagatg tatggaaaaa tgtgaatttg aaaaatttac
    13561 aggaatatat cttgcctaca cgaggaagag ccgatataat tctccataaa actgaaaatc
    13621 atatcatcga ttcgattttc atgcgaaaat attagcattt taaaatagtt tgactatttt
    13681 gagataaaat ttaaagggat aggggtaata aatttgacaa acgttgccga tatgacgaac
    13741 gttgaaaaga ttatcgtatt agactacggt agtcagtata accaattgat cacaagacgt
    13801 attcgtgatc ttggagtctt ttccgaatta ttgagccacc gcattactgc agaagaagta
    13861 aaagcgatga atccaaaggg aatcattctt tctggcggac caaacagtgt atatgatgag
    13921 aatgcatttg ggatcgatcc tgagatcttc aatttaggta ttcctgtatt agggatttgc
    13981 tatggtatgc aattgatcac gtacaaactt ggcggttccg tagagcctgc taaaaaccgc
    14041 gaatatggga aagccttgtt agaagtaaca agcgatcaag ctgtcttgtt taaagaaaca
    14101 ccaaaacaac aaacagtatg gatgagccat ggtgacttgg taactaaagt tcctgaaggc
    14161 tttgaaacag tcggaacaag caaagattgt ccgattgctt ctatccaaga cacaacacgt
    14221 aatttttatg gtatccaatt ccatgcagaa gtacgtcatt ccgaatacgg aatcgaattg
    14281 ctgcgccact ttgcctttga tgtttgtcac tgtgaaggta actggacaat ggataacttt
    14341 atcgatatgc aagtagaaaa aatccgtgaa caagttggtg acaaaaaagt cttgcttggt
    14401 ctttcaggtg gcgttgattc tagtgtcgtt ggggttcttt tacaaaaagc gatcggtgac
    14461 caattgacat ctatctttgt tgaccacggt ctgctacgca aaggcgaagc ggaacaagta
    14521 atggaaagtc taggcggaaa attcggcttg aacatcatca aagtcaatgc aagagatcgc
    14581 ttcttagaca agttgaaagg tgtatctgac ccagaacaaa aacgtaaaat cattgggaat
    14641 gaattcgtat atgtttttga tgacgaagca acaaaacttg ctggtgaaga aggcgtctct
    14701 ttcctagcac aaggaacgtt gtatactgac gttatcgaat ctggtacaga aacagcacag
    14761 acgatcaaat ctcaccataa tgtaggtgga ctgccagaag atatgcaatt tgaattgatc
    14821 gaaccattaa acacattatt caaagacgaa gtccgtgaat tagggactca gttaggcatg
    14881 cctgattcaa tcgtttggcg tcagccattc cctggtcctg gtcttggtat tcgtgttctt
    14941 ggcgaaatca ctgaagacaa gctagaaatc gtacgcgaat ctgatgctat cttgcgtgaa
    15001 gaaatcgcag ctgctggact tgaccgtgat atctggcaat atttcactgt tcttccaggg
    15061 atccgctctg tcggtgttat gggtgacgga cgtacttacg actacacagt cggtatccgt
    15121 gctgtaactt caatcgacgg aatgaccgct gactttgccc gtatcccatg ggaagtcttg
    15181 caaaaaatct ctgtacgtat cgtaaacgaa gtagcccacg tcaaccgtat cgtgtatgac
    15241 atcacttcta aaccaccagc aacagtggag tgggaataaa gagccaacat aatatggagg
    15301 gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc attaaaaata
    15361 agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt cttatcataa
    15421 aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca tctaagtgct
    15481 caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact ttatgttatc
    15541 tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact ctctttagca
    15601 taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat gactggaaat
    15661 cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct tctacgctaa
    15721 cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc tattgtttca
    15781 ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta taaaccccaa
    15841 tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc cattcacttg
    15901 ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat aactgattag
    15961 tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct ttgcttatgc
    16021 cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt
    16081 agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa
    16141 accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa cttgcatttt
    16201 taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc aaggcgtttc
    16261 cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat tgaacactga
    16321 gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa agaggttccc
    16381 aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg gcaagatcca
    16441 gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct gcttcggctt
    16501 caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa gctgtttcaa
    16561 ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt tgaagttcat
    16621 tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat gacacacctc
    16681 gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca tcagctaagt
    16741 ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata ccagacttca
    16801 aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg ccataaggcc
    16861 agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa acgtccagct
    16921 gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc atttcttcaa
    16981 aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc caaaaatcac
    17041 tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt gcaaggtcaa
    17101 tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta ttataaggat
    17161 cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc tctaaggcat
    17221 cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct gacaggatcg
    17281 aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt gaaacagccc
    17341 ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt cccattttca
    17401 ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt ttcaggatct
    17461 ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc taagcgatcg
    17521 gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac caccatatca
    17581 aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact gatcggtgct
    17641 ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcccacttgt ccctgcgcca
    17701 gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg actcgctgcc
    17761 acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat cacctctgct
    17821 ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg tagatagatc
    17881 aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc catgcccgtt
    17941 cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat aaagctgttc
    18001 atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa
    18061 aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa tggttcgttg
    18121 acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg cttagactta
    18181 gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc catagttcct
    18241 acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga attgaacaat
    18301 tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc cacgatcgac
    18361 ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat agcgattcct
    18421 aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc ttctgcaaat
    18481 gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa
    18541 gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc tttaggcatt
    18601 ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa aaatgcagcg
    18661 acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc accttcaatc
    18721 ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga ggtggtgttg
    18781 attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg agcagtggtt
    18841 gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca tagccatgtg
    18901 gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa
    18961 atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa
    19021 taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt
    19081 ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat aaagtcttga
    19141 atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat aaaaatgaat
    19201 cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg cgaattatgt
    19261 gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc aaagtttcct
    19321 tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga atatgcaacg
    19381 atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc cattatcact
    19441 tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga ttcttttggg
    19501 taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt atacccattt
    19561 gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt taccccggct
    19621 gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct cggctgatcc
    19681 ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt gatatagatt
    19741 ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag accttctgga
    19801 taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg ttctccgatt
    19861 cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg gattgcattt
    19921 tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa gtagtcatta
    19981 cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata gctggcctga
    20041 tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata gcctaaatag
    20101 gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc atgcttatct
    20161 tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc aatttcaccc
    20221 ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat catattatga
    20281 agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac acctaacaaa
    20341 tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt gacttctttg
    20401 aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc tcgattcaaa
    20461 aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc
    20521 gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat tccttctgga
    20581 tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa tcttagacca
    20641 tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata aaaatcactt
    20701 gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc aacagcgcct
    20761 ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc gccaaaaata
    20821 aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca gttttcgctc
    20881 tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa
    20941 taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa aaaccaccct
    21001 aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat agattatcct
    21061 taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg tagaaatggt
    21121 caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg tttctgatat
    21181 gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc gtgtccatgg
    21241 aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg aaaaagccac
    21301 tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga ttcacgaggg
    21361 agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc atttactgaa
    21421 caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg ctcaagcaga
    21481 tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg catttgtcgg
    21541 accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta tcagttcaaa
    21601 tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag cacagcagat
    21661 cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat tcaatcgaga
    21721 tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg atcgccagat
    21781 ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg ctgattcaac
    21841 cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt caacggaaca
    21901 atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca tgatcataca
    21961 aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac gaatacggag
    22021 attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt attgtcagaa
    22081 gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca
    22141 aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt aacgattgcc
    22201 acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt agcccgctgg
    22261 tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc gcaaccggat
    22321 atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct tgttatcgaa
    22381 gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt
    22441 gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa agatggaacc
    22501 atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt taattcttaa
    22561 acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt tataaaaaac
    22621 tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat agcagtgtat
    22681 ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat aattaacatt
    22741 aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag gagtactttt
    22801 ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta gttcctctcc
    22861 accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg aaataaacgt
    22921 ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt aagtatcatt
    22981 aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca tgcgtacaat
    23041 tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac taatccctga
    23101 agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta atgactggga
    23161 ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc tatcggcata
    23221 ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt tttcccaacc
    23281 aggagaaatt tcattatcag tacttattaa atctattaaa aaccaacccg ttctctgagt
    23341 gttgattcta taat
//
LOCUS       DAZUKA010000032        23233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000032 DAZUKA010000000
VERSION     DAZUKA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.032"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            438..992
                     /locus_tag="ACG8PN_000983"
     CDS             438..992
                     /locus_tag="ACG8PN_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861158.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="ACG8PN_000984"
     CDS             complement(1165..1638)
                     /locus_tag="ACG8PN_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861159.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2597
                     /locus_tag="ACG8PN_000985"
                     /pseudo
     CDS             1650..2597
                     /locus_tag="ACG8PN_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2868..3303
                     /locus_tag="ACG8PN_000986"
                     /pseudo
     CDS             2868..3303
                     /locus_tag="ACG8PN_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3319..4152
                     /locus_tag="ACG8PN_000987"
     CDS             3319..4152
                     /locus_tag="ACG8PN_000987"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861160.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4397..5422
                     /locus_tag="ACG8PN_000988"
     CDS             4397..5422
                     /locus_tag="ACG8PN_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861161.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5560..5685
                     /locus_tag="ACG8PN_000989"
     CDS             5560..5685
                     /locus_tag="ACG8PN_000989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861162.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5710..6825
                     /locus_tag="ACG8PN_000990"
                     /pseudo
     CDS             5710..6825
                     /locus_tag="ACG8PN_000990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6908..7804
                     /locus_tag="ACG8PN_000991"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="ACG8PN_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7820..8644
                     /locus_tag="ACG8PN_000992"
     CDS             7820..8644
                     /locus_tag="ACG8PN_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861163.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="ACG8PN_000993"
     CDS             8676..9986
                     /locus_tag="ACG8PN_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFS4861164.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="ACG8PN_000994"
     CDS             10134..11393
                     /locus_tag="ACG8PN_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4861165.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="ACG8PN_000995"
     CDS             11403..12275
                     /locus_tag="ACG8PN_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861166.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="ACG8PN_000996"
     CDS             12287..13117
                     /locus_tag="ACG8PN_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861167.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="ACG8PN_000997"
     CDS             13157..15340
                     /locus_tag="ACG8PN_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4861168.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="ACG8PN_000998"
     CDS             15401..16621
                     /locus_tag="ACG8PN_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4861169.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="ACG8PN_000999"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="ACG8PN_000999"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFS4861170.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="ACG8PN_001000"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="ACG8PN_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="ACG8PN_001001"
     CDS             19583..19792
                     /locus_tag="ACG8PN_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4861171.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="ACG8PN_001002"
     CDS             19785..19934
                     /locus_tag="ACG8PN_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS4861172.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20088..20753
                     /locus_tag="ACG8PN_001003"
     CDS             20088..20753
                     /locus_tag="ACG8PN_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861173.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="ACG8PN_001004"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="ACG8PN_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4861174.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="ACG8PN_001005"
     CDS             22456..22689
                     /locus_tag="ACG8PN_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFS4861175.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="ACG8PN_001006"
     CDS             22686..23093
                     /locus_tag="ACG8PN_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4861176.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7955 a         3468 c         4433 g         7377 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggcttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact att
//
LOCUS       DAZUKA010000033        23103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000033 DAZUKA010000000
VERSION     DAZUKA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.033"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..393)
                     /locus_tag="ACG8PN_001007"
     CDS             complement(61..393)
                     /locus_tag="ACG8PN_001007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861177.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(407..664)
                     /locus_tag="ACG8PN_001008"
     CDS             complement(407..664)
                     /locus_tag="ACG8PN_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861178.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(859..1401)
                     /locus_tag="ACG8PN_001009"
     CDS             complement(859..1401)
                     /locus_tag="ACG8PN_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS4861179.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(1422..2513)
                     /locus_tag="ACG8PN_001010"
     CDS             complement(1422..2513)
                     /locus_tag="ACG8PN_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4861180.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2521..4731)
                     /locus_tag="ACG8PN_001011"
     CDS             complement(2521..4731)
                     /locus_tag="ACG8PN_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4861181.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4747..7275)
                     /locus_tag="ACG8PN_001012"
     CDS             complement(4747..7275)
                     /locus_tag="ACG8PN_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4861182.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7327..7737)
                     /locus_tag="ACG8PN_001013"
     CDS             complement(7327..7737)
                     /locus_tag="ACG8PN_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4861183.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7750..7968)
                     /locus_tag="ACG8PN_001014"
     CDS             complement(7750..7968)
                     /locus_tag="ACG8PN_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFS4861184.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7965..8957)
                     /locus_tag="ACG8PN_001015"
     CDS             complement(7965..8957)
                     /locus_tag="ACG8PN_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4861185.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9036..9947)
                     /locus_tag="ACG8PN_001016"
     CDS             complement(9036..9947)
                     /locus_tag="ACG8PN_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4861186.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9966..10310)
                     /locus_tag="ACG8PN_001017"
     CDS             complement(9966..10310)
                     /locus_tag="ACG8PN_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861187.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10330..10608)
                     /locus_tag="ACG8PN_001018"
     CDS             complement(10330..10608)
                     /locus_tag="ACG8PN_001018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861188.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10967..11467)
                     /locus_tag="ACG8PN_001019"
     CDS             complement(10967..11467)
                     /locus_tag="ACG8PN_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861189.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11468..12013)
                     /locus_tag="ACG8PN_001020"
     CDS             complement(11468..12013)
                     /locus_tag="ACG8PN_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861190.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12010..13266)
                     /locus_tag="ACG8PN_001021"
     CDS             complement(12010..13266)
                     /locus_tag="ACG8PN_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4861191.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13626..13937)
                     /locus_tag="ACG8PN_001022"
     CDS             complement(13626..13937)
                     /locus_tag="ACG8PN_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861192.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13960..15435)
                     /locus_tag="ACG8PN_001023"
     CDS             complement(13960..15435)
                     /locus_tag="ACG8PN_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS4861193.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15884..16336)
                     /locus_tag="ACG8PN_001024"
     CDS             complement(15884..16336)
                     /locus_tag="ACG8PN_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861194.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16338..16703)
                     /locus_tag="ACG8PN_001025"
     CDS             complement(16338..16703)
                     /locus_tag="ACG8PN_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861195.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16802..21085)
                     /locus_tag="ACG8PN_001026"
     CDS             complement(16802..21085)
                     /locus_tag="ACG8PN_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS4861196.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21232..21471)
                     /locus_tag="ACG8PN_001027"
     CDS             complement(21232..21471)
                     /locus_tag="ACG8PN_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861197.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21561..21803)
                     /locus_tag="ACG8PN_001028"
                     /pseudo
     CDS             complement(21561..21803)
                     /locus_tag="ACG8PN_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21916..22152)
                     /locus_tag="ACG8PN_001029"
     CDS             complement(21916..22152)
                     /locus_tag="ACG8PN_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861198.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6778 a         5002 c         3408 g         7915 t
ORIGIN      
        1 taattattct tttcataatt tctcctccat aaaaaataag ttacttttta aatatcttgt
       61 ttatgaattc attccccaaa ggccttttaa gattgataca gcagtccgtt cacttctttt
      121 agacttgtca tacaagtctt ttaaatgatc ccatatcaga ctagtttgtc tatctagctt
      181 caatccatta aacgctaatg aacggttcat tttttctatt actttagata aagctacaga
      241 aatttttata ccttcacaac gcgcatcttg gatttcaata atagtatcat caattagttt
      301 ttgaatttct tgcctatcaa catattcaat cttagaaaga gaatctttta aagctactaa
      361 tgattcaacg atttcttttt tctcttcaga cattttcttc ctccttctag tgacccgtaa
      421 agccatgtag agcagagtcc attgaaccag taattacact atctacaacg ccattccaat
      481 ttactctaca ttttccattt ttcagatttg tcggtccata cggatagcct ttttgacatt
      541 caatgttcgg attataccag ataggattat accattcagc cgcagaagca gattcagtcg
      601 atacaaacaa tcccacacca acaaatccaa tacacatcac actatagcca attagttttt
      661 tcatctcgta tccatctcct aaaaaataga atagttctta tagttattca taagacatat
      721 tttttccgtt tttgttttat cctttctgtc ctataacaac ttttttcaag taaagaatga
      781 catagttttt cttaaaaaaa cagtctgttt ttttctattt cttcggtgta taataaatat
      841 gagtgttcat tttttatttt aatacgcact ccgtacataa taaatcttga tcgcatcaat
      901 cttttgagct tcttgaccta ctttcaattg aagtaataca aattgagtaa ccttattcat
      961 tgtttcttct ccaaataaca aatattgatc cggattctct atatcttgtg tcacctggta
     1021 aagtttccca gttgttttca tttgtacatg cgggtctaca tcaatactat tgtctttaat
     1081 acaacgatct gtcatatagt ccctcacagc ttgattgcgt ttataaacat ctgtgaaatt
     1141 actccatttt ctagcaaaat cttttaagaa attttttgtc atttcatcta tttgcatttg
     1201 ttgcgctgtc tcgtcttcta atgaaccaat ttgactgtta ctttcagtac tttgaagagt
     1261 tccacttgtt tcttttactg cttcgattgt ttcttctgtc gttctcaatt gtgttttttg
     1321 tttttcagaa gaaaaccaat gacaaccagt aagacctatc aaacataacc aactaaatac
     1381 taaaattatc ccttttttca cgtttctagt cactgccttt cttatttcac cttacgaacc
     1441 agactacgaa tttttgtttg attgtacgtg cgttgatacc gagccataat ccgtccttgc
     1501 tcggcattct gttccacact agagaaatgt tgtccgccat atgaaacatc tgttataatt
     1561 ccggtgtgac catagattcc aggagacaat gcaccacctg cttgccaatt caccacatct
     1621 cctgcttgta aatcacttgg ttttgggttc atgattactt gccaaccata ggttgtccag
     1681 ttataatctt cccctattaa catcgcaaac atgtgtccag aacccattaa ctgtgggcca
     1741 cctaattgag atacatacca tgccgtacca ccataacatt ccccattatt tacggatcgt
     1801 ccaatgatcc gatccaaaac cttcgcattg cctttacttt ttcctgcaat aagcgaagtt
     1861 ttttttgttg gtaggctatt ccaatgcttg atcgcaaaag tatcatattc cgttaagtta
     1921 taggacgtta tgatactcat tgctcctcga taatacggac tcccttcggt aatacctgct
     1981 gccgcatagc ctccacgacc tagaatcaat aaacaacttc caggatcttt attgcccaca
     2041 cattgattaa agttgctttg gctcgccacc ataaagccat aatagtcata acaagctttt
     2101 aagttcggaa acttcatata gaaaccacct tcagaaccac caattccacg tgcagaacct
     2161 tgtgggtagg gacaaccagg aaaccaggta atcccaaaaa agttcgtatc gtttcttcca
     2221 gattgtgact gtccataagc tgattcatag cctaattgag aaagaatcac actcggtaat
     2281 aactgatact ttacagctgc accaactgct gcttgcgcat gttcatctgt gattggacca
     2341 cgtcctttaa tctctgaaaa aatcggtaat ccttcggtat attccccatt aaaagcgaca
     2401 gctagacctg tagcttctcc tccatgctca gaaagttctc ctgaaaaaaa gaatggcaat
     2461 agaaaaaagg gaagaataaa gaaaagaaga caacctagac accctttctt catttcttcc
     2521 tcatcctttc ttcgataatg gtttttggac tgttcgtcct tttggtaaag taaaatcagt
     2581 tcctggtttt gcgtccaaaa tcggcagtgt tttaggatcc ttttctattt tttccgattg
     2641 ctctgttggc ttatctaaag aagaaaatgc aggttgtaac gattttggtg cggaaatcga
     2701 cggtttactt tcagacgaac gttgccccgg ttgttgcaca gaacgatttc ctgatagagt
     2761 ggctaccgac gaattaggaa cagagtattg gttcaataac tgctgcattg ctggataact
     2821 cccatttttc gcctgatttt tcacttggcg aatctgttgt gaaagagaac cagcagctgt
     2881 taattgtggc acgggtactt ttccagaagg caacggaatc gattgaccaa caaaagcgat
     2941 acctttattt aacaatggtt ttctttgatt attcccttgt tgcttactca attgtgccaa
     3001 tgattgtacc tgttgttttg gtcttactga attcatggac gaatttggaa tcaaaccatt
     3061 tgaaagacca gaattccact ctgttttcga tttggtagaa gacatttcag gctctacccc
     3121 atgataacga acatgacgac cactataagg caaggcttga tttgaacgcc cacctagttc
     3181 aaagggatta accacttgcc tcccttttac cgattcttgt ggtgtacgtt tttgttgaaa
     3241 cttttgaaat ccatgtatcg ctgcagaagc gagtggattc gttgctcgat acacattccc
     3301 tgctaatcct gcacctttga ttgccaaatt ttgtccagca gttgatagtt gaccaggcag
     3361 ttgtttcatt tgagacaaag aatgacttgc tatgtttttc gtgttgttta tagtttgatc
     3421 tgcagctcct aaccctagtt tctttaataa atctttccgt tttacaatga gtaaaattga
     3481 taggagtgct ttgacaaaca tatttcctaa aaaaccaaaa acagtattca ttggaattaa
     3541 atcgtctact atgttataga tcaaaaatgc caccaaaata atcacactgt aaaaagattt
     3601 ttgaaacatc aagcccaaaa aggactttaa ggtattcatg agcatataat caaatactgg
     3661 aacaaaagaa gcgagtaatg aaaaaggaat cacaagtaat aaacacaaca ccaacatttg
     3721 aaagataaaa cgaatcatgc caatcactac aagtggtaat ccaatactta gattaagcaa
     3781 tggcaccata gaaccaacga ctgctttata aacagcttta gttggttgaa tatagacacg
     3841 ataagccttc tttttccctc tatcttctcc tgcagcagtg gtcacttttg tctcaatatc
     3901 agataattct tcacttgacg cttccttggc tagaaattct tgtggttcaa tctctgcttg
     3961 atacaattct tctaaatttg ttgtcccata atttaacaat atatacggat caacaacagc
     4021 cgctttataa taaagttggc gcactttttg aatggcttcc ttttgtgtcg ttggcactaa
     4081 aacaccattg acatcttttg tcgccaattc atctagacta tttgtagcag aaaaaataaa
     4141 gccttctacc tccacggaca aatcatttaa ccctttggta aaagcttctc ctttactagg
     4201 tacgttacta ccatagccaa accaaaccat tgaaaaaatg acaacgaaga gaaatcgaat
     4261 aaacaccatt tttgcgtaat gtttccctct aaatatacga agagtaaata gatataacac
     4321 atacaagacg acaatccccg caccaatagg atcgtatatt tttgaataaa tagtatccga
     4381 aaaagtgaaa aactggtgaa ttaactcgtt cattgcagct ccttcataga gcttctcaat
     4441 gacaaaatca aggccttgcc aaatcaattt acttaaacta aagaacatat tcgtcatcat
     4501 ttttagaaaa tctgtgccat ttgccccaaa taacattttg cctaatgaaa attcactatc
     4561 agataaataa gaagaaaagg tatcaatatg aaatttccca atctcataga tcgattgatc
     4621 cacctgcttt tctgccgcat acaccaatcc accaacaaaa agcagtccga atccaatcgt
     4681 taaaaaaaac aaaacgcacc atttcttttc cttatgcttt atataagcca ttgtttcacc
     4741 tcatttctat tgcatgatta acgtatcctg ttcttctact tgagagtgac ttgttttttc
     4801 cgttgtttgt aatgcagcgg tcatttcttc aaacaaactg tgtacattga gttttcctac
     4861 acgtccatag ggatctaaca tccaacactg tcctttaatc atgtggctca tttcagctaa
     4921 gttcgcttca tttacttcca aacgtaaatg attcaaaatc ttttctcgct cactgtcttc
     4981 atcaaaagca aagacgacac caaaattccc tttttcttta tctgaaccta agtcaccaat
     5041 aaattgtgta tctaacacta attgattcat ctgggaacgc cccacacgtg ccattgctgc
     5101 aataatacta cgtcctgcgt tcgaattatt aaatacccat gcttctgtaa aataaatcgc
     5161 tgttttctta ctactatctc gttttccaaa catttcacaa aaacgaccta aactaatcat
     5221 caaacacaaa gactttcgat ccgcatctgt atatagttct ggtcggacat tgtcatttgg
     5281 taatttcaaa tcttggatct ctaaaatcgt tgttttggca ttaaagtcta atcccgggtt
     5341 ttctccataa gaaaatccta aacgtaaaat cgaatcttct accgtcaagc gtaaaaactc
     5401 accatattct cgaacctctt tttgttccat ttgttctaaa tagtcgacca catgcatcaa
     5461 gccaacttgt tgtccggctt ctttttgaac taaaacttct tggattccct ttaataccgc
     5521 cccttttaaa aggtgattgg tacctaacgg acttaactca tcaatcatat cctgggctac
     5581 ttgtttcgca tctactcggg ataaacaaac aattggatct aatacaccat aattacgggg
     5641 atttgtagca tctaacgtga cataactcat tttttgcaaa tgttctacaa acaaagggta
     5701 atgtttctga taatacgggt cttgaatgag tttcgtaaac catttgcgct tttctccttt
     5761 gggatccaca aataaacttt gtacattcat aaacgaacta tacaaaaaga gcaagccaac
     5821 taaaaaggtc ttccctttac cggtttttcc agttactgct acatgaggcg catcaaacaa
     5881 cgcacctttg attccttccg caatggctag aagattcaaa aagacaaaac gatttgacgc
     5941 ataaattaat tcttctaatg gtgttttttc tcctcgagaa ccaccatccc ctaatgtatc
     6001 ttgccgacca atataccagc cggattccat acctaattct gatactgctg aaaacaacca
     6061 ttcgccaact gcttctacag ttgttcggtg cttccaatgt aactgccgtc ccaatggttg
     6121 ccctaataaa catttataga acaattctcg ttgatcattt tttgcacggt ggatatggat
     6181 aatcgtatga agttgttgaa taaccgttgc actattttga cgaacttctt caaccgtttg
     6241 accgtataca ataaaacatg cgatccaatc gacgacaggt tgcccattat ctactcgttg
     6301 ctttaaatgt tttactaatc ctcgaacagt taataagcgt tcactaggtt cttcatcatt
     6361 ttgaaccaat tccttatgct ctgttttgaa ccgttccgat agccaattca gttttaaacc
     6421 aatacctccc tgtcctttca agcgtggata gtgaattttc attcgcaatt ctgctgggaa
     6481 agaaagatcc tgagcaaatt gaaatagatc acaatttctc atattttcag gtgtttcccc
     6541 aataggcaac aatgctaaat aactactccc taactcatct tctaattgaa gaaggccttt
     6601 atgttccttc ggtatacgta catacgttcc ttgattgtct ttcagtactt cccaattatt
     6661 ttgttcttct actttgtgag ccattcctcg aatatattgc agacgattga gatacaacag
     6721 ctcttttttt actaagggac gaatagaaaa gctataaagt aattgtgtca cttcttttac
     6781 ataaggatca ctttcagaaa acaaagtttc caaatcatct acttctaatc caaataagcg
     6841 tgctaaactt cctgctaaat ctaaccagat tagatttatt acatcatgaa aactctccac
     6901 cgttgctttt tctttgactt tgattcctaa aataaatgaa ggacgataga cttgaccttc
     6961 tcgttccaac aatgtcactg tttcttttgc ataatacgca ccaattgctt gactatcggg
     7021 agaaaatccc tttcctaatg tgtcaaactt cgtccgtaaa ttcattgttc gtggatacat
     7081 caccaactcc acatcttcat aatctgctaa aagcaacagt aaacgagcca tcatttcttt
     7141 tatttcctgt gtcttttttg gtgtgttttt tgaaattgtt cgtggaaaaa catgatagta
     7201 ggcccagata caatcctctc catttaacaa acaatttttc ttgaatgctt ttactggttc
     7261 ttttacaagc accaatgaat ttcacctccc aataaaaaag gaacaaccag ttgattgtcc
     7321 ctttttctaa tcctctaaat aaacatactt attcacaaaa agtaattctt ccaatgcatt
     7381 gtttgatacc ttgtcccagt tataatacac acaatcacca ttcatcatac gaatcatata
     7441 acaaaaccaa tcccataaat aaaaatgcac tttttttcca tcaggtttta gttttgccag
     7501 tgctaacaca cataaatagg ggagtcctat ataaaaaaca actcctgctg gtggaaaaaa
     7561 agaaatgacc caaccaataa atgaacgtaa aaataaatat tcaaagatta cgaccaacaa
     7621 aaaagttgct gcattgacta atgcaactgg aaatggaaga cgccaattgc ttgtaatctg
     7681 ttgtacgcga taaggttccg cataaacaat cgaataatca taaggttccc aatccacttc
     7741 actatcctcc taatccataa ataatttttc aatgatttta ccaaacgcat caaatacacg
     7801 tgttggatca tttaagaaga aataaactgc ggcacctgct gcgatagata ttaatgcatt
     7861 tcctactttt gatttcataa aatttcgcaa tcccagataa acaaccacca ttattaaaaa
     7921 atagccacct tgcgttaata taaaatctac aattcccttc agtgtcattt ccgttcttct
     7981 cctttcctat ccaaccaatt atgtgttaat ttttcaacat aatactgatt tgtttttttc
     8041 gtaatgacaa tcgtcatttg ttctttttgt aaaagtgacg tatcaccatc tcgaaagacg
     8101 accgttgctt ttacaataaa aggttttttc gtttctttat aacgcaatac tttcatctct
     8161 acttgatcaa ctttagctgt ttgattgagt ccttcaggtt ctttcatcat gtatgacata
     8221 tcttttgcat tgtttttcgc atacttatct aagaaatctt ccacaaatcc ttcaacatct
     8281 ttcgcttccg acgcatcaac cgtttcgtat aatgtgtcat cataagtaat cattcgttgt
     8341 ttttctgtat tctttggtag tgccgtaaaa taaggataag caataatacg acagcccttg
     8401 tttgaaatag aaaagggaag attgagtact tgtgtgattg tcacacttgt ttctttctta
     8461 ctctctggat tcatcgagcg aatatcataa gttactttat aactagcagt tggaatggtt
     8521 tcaatttctt ttaatccaat aaacgaagca gcagttaatt ttcgaacacc tgaaacactt
     8581 cctggttctt gaataaagtt ttgtgcataa aactcttttt ttaatgtttc ttgacgttgt
     8641 tgttcttgtt catgattatc gaatgtattc atatacaaag caacaaaacg attcataaag
     8701 gattcaattt caggtgtgta cgttaattct gaccgacttt gtacttcttt cttttgtaat
     8761 tgttggattt catctttcaa aaacgacact tgaaccttcg ttttcatgga ttgaaaaaat
     8821 gccaagcccc ctgaaaaaca aagaaataaa accaaacacc aaatccattt cactgctttt
     8881 tttgaatgga ttttcttttg cttcggtact ttctctgtat aaactttttc tttccgccgt
     8941 agttgtcgtt ttttcataag ctactccttc tagtatgttt ttactagtac gtacgattga
     9001 caacgataag cttgtttttc tgaaaaatag tccattcaat taccctcatt ttctattcct
     9061 tttctctttc ttttatatcc aacaaatcca gcaatacttg tcaacactcc tcctagaaca
     9121 gaaagtgcaa catgtatttc ttcaccagtc tttggtaaga cttttgtttt tccatctgct
     9181 gttttgattt caatattgcc acttggaagt ttctttacag gaatcgacaa atcctttacc
     9241 aaattccctt cactatcttg gattaatgga ataccatctt ttacaccaac aattgtttga
     9301 tcacctgcta cttttaatgg ggtggttggt gttggaatat gggtcaattc ctctacatta
     9361 ctagtgacaa tcggtacact cattccttga gaagtattcg tcgtaatttc accgcttggg
     9421 gttaccgata ctgtgacttc ttttgctgaa acaataggtt tattttcttt tggaattgct
     9481 tctgattttt caacaggttg tttcggttct tctttttgtt cttcactggt agacgacatc
     9541 gttggctgtt ccgtacttga cgaatcagta ttcttttcag aagaactggt tgttcctgat
     9601 tgttccggag ttgtgactat acttgaatcc gttgttgttg aactatgcac accttcttct
     9661 tcgatcgaat cagtcggtaa agttgattca ggaaactctg gttctggtgt cactggctct
     9721 ttttcaggtt tctctgactg caattgttca ggttctgctg gtttttccgt tgttcctcca
     9781 tttagctctg tctcaacatc tggtaatcct tcccctggca atgtttctac aatttctgac
     9841 ttaggtgttt ctacagtttc atctgcataa accaatgaac tccctaattg aaacgaaaat
     9901 aatgaaattc ctagtgttgt tacgactgct aatgttgttt ttttcatgat tattcctcca
     9961 attttttatg caattcgtgt tttgttcata gacgccgcac taatattatc tgaacgataa
    10021 aagaactccc gtccgttttc ttctaaccca acatatggat tatttttgaa taattgaatc
    10081 aatagttcac tggtgttgta ttttggatag tttgcaaaaa aataatactg tgcttgtcgc
    10141 ccatccgaaa attcaacaag taattcataa cgataccgct tcaatccttt tcgttgtttt
    10201 aaaggatctc tataccaact tgtccatcgt tccattcgtg gatagagata gacacgatac
    10261 acatagaaca atgccatacc acataccacc aaaaataata atcgccacat aaaaattccc
    10321 ccttttcttc tacctttctt gaatcatcga acattcatac cctctataga gatttgaaaa
    10381 atccatcatc aattgttcat ataaacgttg ttgcaaatag cgattgtttc gaatacataa
    10441 ttcttcatag ccatcaatca cttgatcttc tttttcatag catcttgtag ctctatgacg
    10501 ctcagcttgc aagacataac caaactctgc accatatcgt tctactgtta ataactgttg
    10561 actacgcaat aatgcaatat aacaatcaca aaaatgttca accgacattt gatttttctc
    10621 acgtttgtga aataggtatt ctttagaaag cacattattc gttaaacaat ctgataaata
    10681 gcgataatct ggcggtcggt tattttcatc tgtgaatttt tcccataata aactgatttt
    10741 tattcgttca gcggctagat agtcttgttc gcgtggataa aattcatcaa tcgcacaaag
    10801 ttttttatca cctgaataaa caaatccata taatgcgaaa cgaactcttc cttttcaatt
    10861 ggctctccta aaaattccag tgacatacca tcttctcctt cctaaaaaaa ataaaaaagc
    10921 agaaaccgat ttggcttcta cttttctttc cttttatagc tcttttttac ggaagaatcc
    10981 gcaaatgaca ttttttcaaa aaatcaatcg gcacaagttc acgaaaactt ggagcaattt
    11041 tggatagaat cacttcccca tcttgtaaaa tacaatcata aaaatcatca gttacttgat
    11101 acttacgctt cttacaataa ttgataaaat agtagcgatc attttctatt tccattcgtt
    11161 gttctactaa gcgcatacga acatcatcat agaaataagc cacaaatcgc ccttcaagtt
    11221 catgtttttc aatgggatac tcttctggta acaatctatt ttttcgtcgt ttatacattg
    11281 ctggatacct tagaagaagt tcatctaaac gtctgtcagt atatggtaac tgacgaatca
    11341 acgattctaa tcgttcttta tacagataga ataattggcg caagcaatag tgtaccgcct
    11401 ctggattttc taatactgtt ggatcacgtg accaatacgc accaacataa atcataattt
    11461 ttttcattta gacaatcaca ccagcaatac taatgaccga tccacaatag gtagaacaag
    11521 tggcatagcg accaatttcc tctaattcta tctcaatctc ttctaaagaa agatatgtct
    11581 gttcttctgt tggctggcga ctcatttccc aagtgacagt caccaatgaa atttcttcca
    11641 taagaggttt ttcgctgcta agtgctgcca cacagttttc atatatcttc attttatcgc
    11701 agcctttttt aaccgctaaa atcgcaggta ttggcaatga aaatcgataa aagcgaatga
    11761 attctagcct tcctttcctt cggctaaatt ccaactcttc gagttcttgt tcgtatccct
    11821 tacgaacttc taataagcgt gcaatttctt gccagttctc tttttcctga taacgtccag
    11881 cttttaccgc ttgttcaata taaggaactc gaaaacgaaa ataagacaaa tcaaccatct
    11941 tttcatttaa tgcttcgatt gcagccttta aatccaacag gcgcagccat tctgttgtcg
    12001 tatatgcatt catgcaattt tccctcctga cgttcccatc cattctagat agttttgttg
    12061 ctcgtcattt agttcggcat tatcaatccc ttcttgtaca aagtctcttc ctaaaatacg
    12121 atctgcttct tgtaacaact ttaatgtcgg agctacttgt ttcaaaaacc agcgttctga
    12181 acgatctaat gagaattttt ctggctttac tgtaaaatcc aattcttcca tcgaacgaaa
    12241 taattttgcc catcgtttat caacaatccg tgcatcactt tccagtctat cccaaacaat
    12301 aagttttgca ttaattaaac caacgccaat ctctcctaaa cgtttcggtg aatcacctaa
    12361 aataaatgct tctactgctt gtgttgcttt ctcattggct aaccgtaact catatcgatt
    12421 ataaatacca tatttcgtta atgcttctaa ttctgaaatt ttttctcggt tcgcaatttc
    12481 aaaacgtttc tcataaaaat taaaaaagag aggactcttt cgcgatccaa aatacaaaga
    12541 taatccttca cttcgaaaat agccatcatc taaaaaacaa ccgccaatat tctggaatct
    12601 cttaaagtcc atttctaata aacctgcttt ttgtttttct agtaattcaa agagatcgaa
    12661 atgttcttcc gtttccaatc cccaaagttc atctaacgca atatcgaatc ggctacattg
    12721 cacacgtgta taaccagctt ccaactttcc ttgatttttc caaatggaat atagccatgt
    12781 tcgccaatct gtttgttgtt cacgcatgac ttcctcatat tcccggcaac cttgtcctga
    12841 aaattgcaca agtacatttt ctgttacagt tcgttttgaa tgccagaaca cccagatatt
    12901 cccacgaaca gaaagacagt caaaaagata caagcccgtt ggacgtgttt caaattctgt
    12961 cagatccatt cgtaatacac gttgaatcac tgttttgtag tctgtcccaa aaaaggaaag
    13021 acgcaaataa tcaatcattg ctcgaagatc tattccgctt ttcatataca atcgaaagcg
    13081 ttccttgatt cgttgttcaa aatcctctgg gatttttctt ttcccttgtt ccatctttct
    13141 aatgtaatct tttgagcagc ccatcaattg cgcaaatctt gcttgtgata gcggcttttt
    13201 atctccactt atattatgaa ttgcacttaa cttctttctg tagcttttta gctcaatcgg
    13261 tgtcattttc tcgctccttt tataaaataa caaaatcgat ttttttaacc ctttagaaac
    13321 accgatatat caactagtcc ccctcgtttt ttgggaaaag tagtacaact gctgacctta
    13381 gataatcact tgatacaaaa gggtttataa aacttttttc tatgtgttat gttatttctg
    13441 ccccccatat tatatatcgg ggggctttac acaaagaggc cgtgcgcaag cgcacgacct
    13501 ctttgtgagc tgcacacgct gctagcgagt gcgcattata ggcccatgct tcgcagcctt
    13561 aatgcgcacc gccaggcaga gtgcggctac cagactacgt ctagagccct cccgatagat
    13621 aataactaca aaattaaatg atctaatttt gttatttcac tatatttttc aggagaaaaa
    13681 acgcgataaa ttgacaaagt taaatcctct tctaaatcta ctcgataatg atacaatcga
    13741 gtcaatcgtt ctatatcttt tttcgttagc aatacttgat taataacttt tcctgtaaaa
    13801 acacgttctt tgcgcaaaaa gcggtaatca gttacttgtt caatacgatt caaccaatca
    13861 taaaacgttc tggaagaaag agaaggatta atcattcgta ttgctgtagc tactctataa
    13921 taacaactct ctgccattta acttcacctc cttagttgat tacaaaatat gcgcatcaga
    13981 aaaacttgtt ccttttggat cttttgctaa atacatttgg cgctctagtt gttcttgtaa
    14041 caactgatta tcatctccca tcgtttgatc ttgactatcc tcttttactg taaaatcata
    14101 gacaatcatt tcagtaaatt tctcaaattc tttgataaag tgataatctt tgtaaacaaa
    14161 tggcgcaaaa aactctacag gtacatcaaa tcctggaaaa gcatatccac gaccaggttg
    14221 atctttgttg taaaatggct tatttttaaa atcttctcca aaagtcatta aataaccaat
    14281 gggagacaat cgaccaacag aaatacggaa tgttaaatta tcccgaacac cacttggaaa
    14341 atattctgca tctggtcgtt gcattgctaa aataatgtac atccctgctt gacgaccctt
    14401 taatactaat ggctgaatca atttatcgac tctatttctc gcttccatat ccaacgaacc
    14461 ataaaaagct gcccattcat caaaaaagat aaaatgcggt ggtaacccat aataagcata
    14521 atttttccct ggttcatatt taggatcttt catggttttc atataggcat accgttgatt
    14581 catttctttt aatgcatttc gtaaacaaat aatcatttct tcatttattt tgtaaaacac
    14641 atgtcctttg aatacatcga cgtcacctaa tgctgctaaa tctgccgctt ttccatcgca
    14701 aatatctact gtcccaacac ttaaacaagc ttgaattaaa gaatacaata aataggtttt
    14761 cccaccaccg attgcaccag caatcaacat atttggtgat tgatcatata cccaatccaa
    14821 agttttcatt aaatgaatac gtccattttc tgctatacat tgatcaatag gaatccgacc
    14881 agaatctgaa aggaatacat aattgattcg ggctttttct cgttcttcac gaattaaatc
    14941 acgcaaaaat acttcagata acaaatgacc catttttaaa aatcgttcgt gaaaacgccc
    15001 accatctaaa ggaaaagaaa ccactaattc ttgtttcttt tgataaaaat aaacttttgg
    15061 taatctcatt ttttcccttg tttgtttctc actaacttgt ttctttgtat aaacaaaccg
    15121 attgtcaatt aacattcgaa caagtaactg acgccaataa acagaggcaa aatatccttc
    15181 ttttagtcgc gaccaatgca tcaaccctaa aattaaccca ctaaccagca acaaatacaa
    15241 cacaatactt attccaggaa taagatacat ttgccaattc gaaaacgtac ttaaacgtgg
    15301 ataaaatact ttccaactaa accaacctaa cagaatcaaa aatggaaaca aaaagaaacc
    15361 ataatagcaa aaccataaat aacgattgaa atagcgaatt cgccgaccac gatacttgaa
    15421 aaaatgagct ttcaattgtc acattcctcc tttctgtttt tacttttcct gtgctaaaaa
    15481 cttttgacaa aaagcaacta atacaggaat atcacattta gtaaaaaaac ataatcgaaa
    15541 aagatttaat cgatttttat aagggtgtgc acacatatta catattcgta ctatacgaat
    15601 ccaaatcact tcctgatgat cttgtttcca ctggtacggt aatgaaaaac gaacaagatc
    15661 acgtaaaaac atacactgcc caataaccgc acaaatatat gcatttgttt gtacaaaaat
    15721 cgttggattg tctccaaaat actcaatcaa ttgttggata ttttgataac aagcacaatc
    15781 tctcaaagta tttgattggt tttatctgaa ttcaaaatct ccccttcctt ctatcttaga
    15841 taaaaagaat aggaagactc cttcctattc tttcaaaaaa accttatttt ttctcttttt
    15901 ccttagaatc aacacttggt tgtttttgat ctaaaccttt ttcttgtgat tgtttcatat
    15961 taggattatt tgttggagct tctactttct ctaatccttt cgcataaatc actaaatctg
    16021 aatattcacg attgccatct tgtgctggaa tatataccac atgatattct ggatcaatta
    16081 atttcacagg atcacgtcgt ttaaaaccag tcaaatctac atctgctgga aatttaactc
    16141 gtcgtgcaat ctttggttca tctggatttc caaaaactaa ttgtgctcgc tgtgatcgaa
    16201 tttctcccgt ataatcgcct gtttcattat caaatacacg atcagactct tctaatgcgc
    16261 caaagcgtac ggctcctaaa caagtttcat ctaatgcttc tgtaatgtca taattatttg
    16321 gaaattttaa tcccatctta caaaacctcc ccaattttta caccagttaa aaagacacca
    16381 acatcttgat agccagttgt tcgacgctct gtttgtccca tatcatttgt ttcacgacta
    16441 ttcactcgtt ctttacgaaa aacaaaataa gcttcttcaa atgaaatacg cacctctttt
    16501 tctggtaatc gtaatctttt atcctcttca gctgtgagat aaacaaaaaa agcaggctgt
    16561 tcttctcgcg aacactctgc ttgtaattga acatatcgcc cagacacacc attaatttcc
    16621 tttggtggat taggttcccg tgaacccaat aaaaatactt gacctaaaaa ctctttcttc
    16681 atacgcaatg ctttttctgc cataaagttt ttcctcctct taaaaaccaa aataaattct
    16741 aaatatttct acattacaat caaacaagcg gttaaaaatc ctaaccgctt gttcctcatg
    16801 actaatcttt ggctttacgt actttataaa gtgttatacc taaagcaatg actaatacta
    16861 ctaatccaag aaccataagt atactacttg tttgttcacc tgtttttggc aacattggta
    16921 caacagtagc tggtttaacc cgttgttctg gtttcactgg ttgcggtggt tttggcgctt
    16981 ccacaatttg aaccgtttga tctttgtttg ttagatctac attgtgtttc gccacttctt
    17041 caaacgtttc tttctcatct tccgttttcg cttggagcca ttccgcaaaa ctcaagcctt
    17101 ttcctgcaag attgcgaccg tccactaaga attcaaataa gaattccatc atttctttat
    17161 cggccgcaaa gactttttcc gcatgagtgc cttcaaatgg tgtgccgtct ggtgttaagt
    17221 agccttgaac aattaagcga tagatttgac caatcgcaag accgtctact ttgacccatt
    17281 caagcacagt ttgatttggc aaggccaaga tctcttttcc gccattttta ttcgtcgctt
    17341 tcgtcgtaat ggttggatta ctgattttga tggtttggtc attgtcggta atatctgcgt
    17401 gagaagccaa taatacgcct tctctaaaca gatcttcaaa gaccacgact tctttgcctc
    17461 gcaacgcacg tgcgtctaga ataaagtcta gattgactgt accatccgtt gtttctgcca
    17521 caaacgttaa ggtacttgtg acttcttttc catctactag cactggtttt tccgttactt
    17581 tgtccattag aacaccgttg actgtgtatg tttttccaac gaccaaatcg tggtatttca
    17641 cagcgtctgt aatggtgact ttcgttaatg gattaacctc tttcaagcca gtgggtttat
    17701 gtgtagccgt tgtacgtaca cttgggttag ttactcgtac cgtttgccct ttgtcgttga
    17761 tgtctttatg gtttgctagt tcttcatcat ttctaaaaag actttcaaac acgacgattt
    17821 cacgaccatt tatacctgtc aaatcaaagg taaatggtac gatcaccgtt tcattttctg
    17881 ctttggcaat aaagccaaca ctcgtttcta ccgtatttcc atgcacaagc aagggttcac
    17941 cggttgcttt atccattaag atccctttga ccaagtatgc ttttcctgga agaatctcgg
    18001 taatttcaac ggtatcgttt aaggtaaaca atcctgttgg gtctgcgact gtcaatccgt
    18061 cttcaaagac agccgtggta tgaacggttg gattattcac tttcacactt tgggcttcat
    18121 cgttgatatc cgcatgtgct gcaacttcaa tgccatcacg gaaaagtttt tcaaaaacaa
    18181 cgatcgtttg acctttcaga acactagcat taaccacaaa ttcaaggtct tttgtgccat
    18241 tgactttgtc tgcaataaag gccaaggtac ttgtgatttc tttgccatct tgtaaaattg
    18301 gcgcattcgt tgccttgttc atcaatactc ctgaaatggt gtattctttt ccgacaaaca
    18361 agttggtata agaaacactg tctttaatga cgatcgtttc taatggatca accacttttt
    18421 caccatcggc tttatttgac gcttgtgttt ctagttttgg tggaatcaag taattacgta
    18481 cagaaaccac taacgtttcc ccatcatctg gcgcattttg tttgaatgca accgtgaatg
    18541 cttgtttatc tgtatttggt tggaaacttt ctaatggggc attttccaag aagtaatagt
    18601 catcggcaac tagacctttc actaaaattt ccccgttttg atcagtggta tattcaccaa
    18661 ttgcttgatt tttaccgtta tacaaggtaa agttcacacc tgcgagcggc gttttcttct
    18721 tgttccattc ttcatacttg aataatttta cacttttccg tgcttgacgg ttcgcaaaat
    18781 ggatcacttt gatcggttct gtatcattgg taatcgagaa cactaatggg ttttgattca
    18841 aaacgtaatt tacgggggct ttgatttctt ccaaacgata gcgatctttt ccataagtaa
    18901 gtgtaccgtt agttaggaaa aagccttttt cattggtttt aaagatatcc gttgtttctg
    18961 tatcatttgg aatctccatt gtgacataac gattggctaa agtatcaaaa atcttgaatt
    19021 ctgtattagc aagtggaatc acttttcctg tttcttgatc cactttcacc actttaagac
    19081 gttgttcaat tactttatct tctaaaacat aatggaaagt ttgcccattt tcagaaattg
    19141 tgacttcaag tggttcaaat agtttatacc cttcttttcc tttggtttca gaaagaatgt
    19201 acgtatcaaa gacaacggta tttttaccaa aagtagcata accatcattg tctgtcacaa
    19261 cggttttcac cactttgcca gttgtttttg acgtcaaagt gatttcaatg ccagctaatt
    19321 ttggtttgtt atctggattc gttgaatcga ctaaatcttt attagcaact ttcactaaat
    19381 caatggttcc tttgatcact tgatcttttg cggtcgcatt gacagttttt ccaggttcaa
    19441 caacaatttc aattgcttca tcagaaagca cataaccttt tggcgctttg atttctttta
    19501 aaacatattt gccaggatca agatctttaa tttctgcctg tactttgccg ttaatttttt
    19561 tcaaggtaat ttctttaatc tttttcccat ctgcttgata caaaccatag accgcatttt
    19621 ctaaagaagc tgcaccttgt gctttcacac cagtttccgc atcttcttta aacacacgaa
    19681 tgtttccttg ttttagaatt tgtaaattaa taacagacgc attaggttct gttggatgta
    19741 acacaccaat tgtttgagct cctggacgac gataggctaa tggcgcacct tgaactttaa
    19801 ttttattcaa ttgaattttt gctttatccg gtgcctcttt tgtgactgta atccatactt
    19861 cattgccgtg aatagataca ttgacacctt ttggtttttg aatgtctttc acatatttca
    19921 aaaaatcatt tttattcttt actttcaacg tttcgccaat ttttaccgta tgtgtttgat
    19981 tattaaaagt tgctttttca ccaaagttat taatcaaacg ttgaatttct gcttttcgac
    20041 gatcataatc aacacggata tttttgattg ggatttttgt gcccaaagct tcccaaatca
    20101 taaattgtgc cgcaatgtat tgttcatcgg atttgtcttt atttgtgaca tatcctaaat
    20161 aagagatcaa gcttgttttt ttccgtacat ttgaattttt caaatagttt ttcaaagcag
    20221 tagctgtata aacttgacct tctgcggctg agacaccagg ctcaatacaa aacgccactt
    20281 gtccattaat ctcaaaacga accatataat ttaaagtgaa gtttagattg ttcggactag
    20341 aagtaacacc aatattttcg ccccaatcaa ttttccgacc acctaaaaca gctgcattgg
    20401 ttgcacggcg aacacgaacc gaacgattgg ttggtactgt tactaacagt gtaggtgtac
    20461 ctgtttttgg aatgttctgc tcttttttct tttgattttc aatactagcc gtagattctt
    20521 tgtttggttt cgttggttga ttttgactag ctgcggttga agagattgtt ttttcaacgt
    20581 tttcctcatt ttttccttct ttgtcagcag tagccgctgg ttgttccact gtttcttcta
    20641 ttcgtttctt ttcttccgct tgtttctcag aagactctgg ctgcacagaa ggcgttgctt
    20701 cggcagcttt ttctccttct agagtggacg tttctgaagc aggggttgta attgaggcag
    20761 tttcttcaac tggttgtgaa gtaccaggac tattccctgg gtgttgtgtt tccccaatcg
    20821 tctgatcgag agtcgttttg gaattgaccg ttggcgcttc tgtcacaggg gcctgttcaa
    20881 cagtggcttc accagctggg accccggctt ctgctggttg gacttctgtc acttcagaag
    20941 cgacttgggc aatggcttcc atcgtaggtt gtatagccgt cgtttcttct gcatgtgctt
    21001 gagcagttcc caccaagaaa cagaattgag aaaaagccac caatgcgagt gctttcaacc
    21061 aattcatttt cgtttttgca tacacaagaa ttcctccttt tcctgagaat tttatataat
    21121 aaaacaaaac gccgttacca aaagtgataa cgacgtgaac tgtctcttct atttttatct
    21181 ggcgctctta tctgaacaca tttcatcaat gattttaccc tcccactata attacagaac
    21241 aaaaaaagaa cctgcttcgt ggaaatcaac tgtccatttc ccaattggaa taccagcttt
    21301 tttggcaact tcaccatcac caaatctcat gccataccaa ttactacctt catccttaat
    21361 ttctttccaa ccatatgaca tagcttcatc taatgaatca aaaatcatga cactattgcc
    21421 aattgttaca ggatctggct taagatcttc tggaagagaa atatcaatca taccataatg
    21481 atccgattcc tcagtattat tgtcattact agctactggt ttggttgttc tactggctta
    21541 ttttgataca acaagtactc ttattcttct tttacttctc cgttttcaat ttcccattca
    21601 aaccactccg caaagtcttg cttaaaacca tctactcgat acctattttg taaataggca
    21661 tcttctgacg tcgtaggatc taaataggta ggtttattct ctggacgagc atagacacct
    21721 acccatgttt cgtaatctcc ttcaaaataa ctatctacca aatagccttt ttttctaaga
    21781 atctcccgaa cttgattttt aattattgat acttgttcaa aaccatcact cattctatca
    21841 cctcaaataa ctctagattc tcataaatat tcccgacaat ttcaatccta tcaatgatct
    21901 ctaacaaatc gctacttatg cttccccatt cgtataagaa tttcccttcc gcaaaataaa
    21961 ttttccataa acttcttcat gatcgtccca accaatatca ctctcgaaaa attccacgtt
    22021 gttcttatct tttagctcag taaatgccat aagttcaaca tgttttccaa cctcgaaaca
    22081 atcacaccac ccatcaaccg ttacacgtgt gccttcaata cctaagaaac tttgtcctcg
    22141 tgtattaaac actaaaactt tccgcatttt ttgctcatta attaatcacg cgcgaaatct
    22201 aggaatcaca acttattcgc ttcttcttta taaataaaaa aactccaata atcggaaatt
    22261 actatctttt cctcattcct tttttaaatc ttcatacttc ttcttatctt tgtcctaacc
    22321 taataacaca aattttgaag aaaaactcag taaaacctga ttcttttttt atcttcacaa
    22381 caaccaactt tttcttcaga tccttttact agaacttgta tacaaaaatt gttgaatgct
    22441 gatataattt gcggctactt gaaaaaacga tagatgtaaa aagataaata gaatcatttt
    22501 ttattctata tcagtctatg attcattgct tatcaatcac ccaacccatt cgatgccgtt
    22561 ttttttagag aaatttaaaa aagaccgtac gaatctcgca ttatccgatt acttttccag
    22621 aaaagatccc caattctgta taaataaccc tatcttcttg ttggtttcat tttctcatat
    22681 aagaattcag tcaatctacc cactatgacc attactacgt ccaacacaac tgacatatta
    22741 accacgtaag tattccctca cagatctccg aatctccatg gtatggattc ccatcctgac
    22801 gctgcagagg acaacatatt tcatgtggct ctgtaccctt ttgatttgga aacactacaa
    22861 atcaatactc atctataact tatcaagcga actttcgcta ggcttctagt gattactaaa
    22921 ttgttggata acatacatag cccattttat ctagctgtta ttcataacag ttagttcttg
    22981 tattacttct tccgcttgtg ttgccattga gcttgtcaaa aagttactta gaaaaaagct
    23041 gccagtaaag caactttcaa caaacccttt ttgtttctta tcatatcaag aaacctcctt
    23101 tta
//
LOCUS       DAZUKA010000034        22971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000034 DAZUKA010000000
VERSION     DAZUKA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.034"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..591
                     /locus_tag="ACG8PN_001030"
     CDS             61..591
                     /locus_tag="ACG8PN_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4861199.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(1074..1517)
                     /locus_tag="ACG8PN_001031"
     CDS             complement(1074..1517)
                     /locus_tag="ACG8PN_001031"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4861200.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(1533..2693)
                     /locus_tag="ACG8PN_001032"
     CDS             complement(1533..2693)
                     /locus_tag="ACG8PN_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4861201.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3200..4900
                     /locus_tag="ACG8PN_001033"
     CDS             3200..4900
                     /locus_tag="ACG8PN_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861202.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(5002..5835)
                     /locus_tag="ACG8PN_001034"
     CDS             complement(5002..5835)
                     /locus_tag="ACG8PN_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4861203.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(6139..8304)
                     /locus_tag="ACG8PN_001035"
     CDS             complement(6139..8304)
                     /locus_tag="ACG8PN_001035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4861204.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            8564..10858
                     /locus_tag="ACG8PN_001036"
     CDS             8564..10858
                     /locus_tag="ACG8PN_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4861205.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10851..11516
                     /gene="pgmB"
                     /locus_tag="ACG8PN_001037"
     CDS             10851..11516
                     /gene="pgmB"
                     /locus_tag="ACG8PN_001037"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4861206.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            11509..12516
                     /locus_tag="ACG8PN_001038"
     CDS             11509..12516
                     /locus_tag="ACG8PN_001038"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4861207.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            12538..13557
                     /locus_tag="ACG8PN_001039"
     CDS             12538..13557
                     /locus_tag="ACG8PN_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861208.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(13838..15205)
                     /gene="nhaC"
                     /locus_tag="ACG8PN_001040"
     CDS             complement(13838..15205)
                     /gene="nhaC"
                     /locus_tag="ACG8PN_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4861209.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(15314..16735)
                     /gene="tyrP"
                     /locus_tag="ACG8PN_001041"
     CDS             complement(15314..16735)
                     /gene="tyrP"
                     /locus_tag="ACG8PN_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4861210.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(16978..18855)
                     /gene="tdc"
                     /locus_tag="ACG8PN_001042"
     CDS             complement(16978..18855)
                     /gene="tdc"
                     /locus_tag="ACG8PN_001042"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4861211.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(19146..20402)
                     /gene="tyrS"
                     /locus_tag="ACG8PN_001043"
     CDS             complement(19146..20402)
                     /gene="tyrS"
                     /locus_tag="ACG8PN_001043"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4861212.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(20514..20719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20982..22283
                     /locus_tag="ACG8PN_001044"
     CDS             20982..22283
                     /locus_tag="ACG8PN_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4861213.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(22352..22951)
                     /locus_tag="ACG8PN_001045"
     CDS             complement(22352..22951)
                     /locus_tag="ACG8PN_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4861214.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         6967 a         4751 c         4144 g         7109 t
ORIGIN      
        1 atgagtaatc gaaaacatgt agtttattac atgttttaat ttataaggag atgcttaaac
       61 atgaaagaat ttaaagaatt ggaagatttt attggaactc actttatcta tacgtatgac
      121 aatggttggg aatatgaatg gtatgccaaa aatgaccata ctgttgatta ccgtatccat
      181 ggcggaatgg tcaaaggccg ttgggtaaaa gaccaagaag ctgatatcgt taaattgacg
      241 gacggcgtat tcaaaatcac ttggacagag ccaacaggga cagacgtggc attggacttt
      301 atgccaaatg aaaagaaact tcacggaatg atctttttcc ctaaatgggt tgaagaacat
      361 ccagaaatca ctgttacttt ccaaaacgaa catatcgcag aaatggaagc agctcgtgaa
      421 aaatatgaaa cttatccaaa attacttgtt cctgaatttg ctacgatcac ttatatgggt
      481 gatgcaggta tagacaatga agatgttatt agcgaagaac catacgatgg aatgccagat
      541 gatatccgta gcggaaaata cttcgatgaa aattataaac gagtaaaata aggttgtgaa
      601 agagaggttg tgaaatcagc gttctgacct gagtattaga gaaagaaacg gaaaaatagt
      661 tcctcatgtt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
      721 tcaagaggtt gtgaaatcag cgttatgctt tgagtaataa gaacgctagt tagaaaatag
      781 cttttcatat tttctaggct agtgggctta ttaccgagaa gcagccgttt gaacaccgtt
      841 tatcaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa atggaaaaat
      901 agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
      961 ttatcaaaga ttgtaacaac tgcatattca cacagttaaa ttagtgactt caaaatgaaa
     1021 taatgctccc agcaaaaaat taggctggga gcattatttc gttattctgt tcatcaaaaa
     1081 tcagcgtttt gattccattt catcacaagt tcctcctcat ttactgcttc atctttttgc
     1141 tgtgtttgaa ttgtaaaacc ttgtgaaaga tagaaatgaa tagctgattg atttttttga
     1201 tagactgtca aagtcagtgt tttattggtt tcttttattt ttttcattag ttgcgtaccg
     1261 attctttgtt gccggtattc ccttttcaca aagattcctg cgataaactg atcaatcacc
     1321 ccagcaaaac caataatttc ttgatctatt tctgctaagt aaatcactgc atctggtaaa
     1381 gctttcctaa cagattctac ttgattctcc caataagttg atggaatgaa ttcatgagaa
     1441 tccaagttag catgcagcca aatcgataga acagattcca gttctttggc agacaatttt
     1501 gttgttgtgc ggatcatttt ttctctcctt acttattcat tgattttaaa ataaaaacca
     1561 tttttttgaa gctcttttac aagttttcgc ccctcaaaaa agtatatctt ctcttgtttt
     1621 acttttctaa taccaaaaga aacagcaact ttaggatcat cagctgaaac gattcgataa
     1681 gttccaccag gcaattgatc cagcttgact ttcccatgat ggtcgctttg tactgaaaac
     1741 ggcacaccat ttctagtaag tgtttttttc ttatgtttca aaatgaatga actaccattt
     1801 accggtttcc ctgacgaatc actaataata aatgaaaaat caagcacttt tttacttaat
     1861 ctatactgat agattagtcg tccgataaac actaacagta tgactacagc tattatcgtt
     1921 cctgcaagaa taaaccattg ccgcatggtt ctttcctgat ttccttttcc ttcttctttt
     1981 tttaccgatt cgttataagg aatccgatgt cctgtcacta gcaaacgatg cgtgttgatc
     2041 atatacggcg tacatgtaag caaggtaact aagtcttctc cttctgaaat cgttagaaca
     2101 gacgtgtcat ctggcagtac cacttgtata tttttaactt gataagccaa tttctccccc
     2161 aaaacagtca aaataaacgt gtctccgttt tctaaacgat ctaattcggt aaataaccgt
     2221 cgattaggta aaccgctgtg agcagagatc actgaatgcg tcgaatttcc accaagcggg
     2281 aaagaggttc cttgcaagac gcctgctcca ttttcaagga tcctaccatt gagccgatca
     2341 taaagtgaaa tatttaattg gatcttaggt atattgatac ttccgatcag ccattcctct
     2401 aattggctgg atgttgtact ttcatttctg ttcttttcat tgaaaggatc agtaccagga
     2461 ttgaatcctt ggtcagttaa tcgttggttt ttctcctcca ttttctttcg ctgcacttca
     2521 atttcagctg cggtccgttt ttgtacttgt tcgatacgtt tttgatcaat aaaactattt
     2581 aaagaatcca cataaaatgg atataaagcg atcagtaacc ctacgacgat acatattaat
     2641 aaaaacaaac gaaacaaaag tcgacgagac tgtttttgct tactctgctt cacatgtgtc
     2701 ctcccttgca agtttttttg ttctttcttt tattatatgg gttcaggaaa aaaatagaaa
     2761 atgaaagccg atgaggttgt gaaaaagctg tcctgcatca agtaataagt acagcccaaa
     2821 aaaatagcta ctcatatttt ttgttgggtt gggcttatta ccgcagatgc aagcttttga
     2881 acaccgttta aggaagaggt tgtgaaaact gcgttatgct ttgagtaata agagcgctag
     2941 tcagaaaata gcttctcata ttttctaggc tagtgagctt attaccgaga agcagctgat
     3001 tgaacaccgt ttaccgagag gtcatgaaaa agctactttg tttcgagtat taagaaaaat
     3061 tctgtttcaa aacaaggaga tgcaacaaaa cttatgtcgc atcctctctc cttagaactt
     3121 tcacttatgt ttttcttgta aaattcataa atattttcat gaattttcta atttcttgcc
     3181 tacctctcgc ataggatata tgatgaattt atctaaagtt tgcaaaggag ttcgacaatt
     3241 gaaaaaatat ggcccatttt tagcaatttt tttcattgct ttggcatttc tcatgcctca
     3301 tttattcaca cataaactaa ttttgggtgc tgatgcccca ttccattaca atcggttcta
     3361 tgatgccgcg caacaaatta aatatgggga tttccattat tttccttccc tttatggatt
     3421 ccagcaaagt ggcaggatcg tgaatgccgt ttacggccca tttttcgctt atttacaagg
     3481 attgatcgtg ttgcttgcag gtagctggtt tcattatcag attctttcaa atttcattct
     3541 ttatcttatt tcaggatttt ctatgctgat tttactgaga tatgtcaatg ttcgaaattg
     3601 ggtaagtgta ccagtagccg ttttattcat gagtacgtat tctattcaat actggtctat
     3661 caaccaagga ttcactagct gggggacttg tttttatcct ttatgtatga tccctattgc
     3721 tgatttcgtc cttcataaaa agttccctgt tatcaaagtc gctgtctcag tgggacttat
     3781 gacacagatc catttactgt cttcgatcat gttagctttg atgtatatcc ctttttatct
     3841 ttattacttt tttaaccaga ctgaaaaagg aaaagcactg cttcaactga ttaagtcggt
     3901 cttgctttat tttgttctga cagcaaatat ttgggcagga ttagctattg tttacagtgg
     3961 gaataagatt cttcctccct ttgttaacag agaaatgagt caaaaggcga tagatctgga
     4021 tggcagttat tggctccatt accctaaggc ctttcctata ttgatattat caggtttttt
     4081 gctttttatt ttctttcaac aaaagaacac cttattccaa aaaatcgtgt tgtacacaag
     4141 tcttgttttc ttttttcttt ctactagctt gattccatgg acgacattga tcgaaaaaaa
     4201 tgtgcctttt attagtttga tccagtttcc atttcgattt ttcgtgccat ttacgctatt
     4261 gcttctactt taccttgccc tttcactgaa ccagtggtct gaacaaaagt ggttccgtct
     4321 tttaagtata tttggcattc ttattttttc agcacagacg atccagaact tataccagca
     4381 tctggaaaag tgggaaaatg aaacatttgt cagtcgacat acttatctat tcgatacgcc
     4441 ggaagaagca aggaaaacat tcttcagcaa agatatgtct accagcttgt tcgttttcca
     4501 aaaaaccaca cctgactatc tgccaatcta taaggagaca aaagacaata aatacgaccg
     4561 gtataacgaa gaaatactag aaaacgaaac aacttttata aaaacacatc caggcggtct
     4621 tttgacaatc aagtggaaaa acactgggga caaaactgtt catatccctg ttatcgtcta
     4681 cgatcggact gtgctacagc aaaatggaaa aacattgact gattatgaag taacagatat
     4741 cgggacccca atcgtgaagc agcaaaaagg aatcaacgag ctcacgcttc attatcaaac
     4801 gccaatctat ttctatttca ttctttctct tactttgatt ggctggttta ccttgctatg
     4861 tttatttatt tatcatagat acaagctttt acgtgcttag gcaaaaggga taaatacatc
     4921 cgatcaaaaa actctccaaa cggtataatc gtttggagag ttttatttta atcagttttt
     4981 ttatgttata tgttatattt tttattcgtt cacatctaat tcgatttcca ccccgtagtg
     5041 atcactgatg attggactat cgatcccatc aaaaatccgt tgatattttt taacctgcat
     5101 tccttcaggg acgaaaataa aatctatacg caatttttct gtattttgtt cccaaccatc
     5161 tattttcttt tccacagtag ctgcttttcc tttttcattc gcgacaacat acgaatcttg
     5221 aagaagaagg caactttctc ttacagtata ataaccttga gagtctactg gattattgaa
     5281 atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag tagttttcca agtttttcca
     5341 ctcaaaataa aagccacttt ttttatctgt ccaccaagaa aagtggcagc tcgagacaaa
     5401 aatgtcctga tcatctatct ttgttttccc aatcaagatc ttgcgtgtct gataatcttc
     5461 tttcttttga aattgggaga cggtaaggac ctgactttcc aatgtttttt tcgtaagcag
     5521 tgcatttcct tcttcatatt tatcataacc gatgtggctc atttcccaac tccaaaaata
     5581 ctcttttccg tgttccttta aaagctgaac taaacggtag gcaaaattat ctttacggat
     5641 aggtacttga ttctttaccg gacagaactt cattagcttc gcaggttcta cttcttcaga
     5701 ttccagtaat tggttgattt cttgtaaagc aatcacttca taatcttcta acaggatctt
     5761 atctgctaat tcttgcaatt ttttctctgg ttctggctca agccagctat gcgtgttgag
     5821 tgtcatgaat ttcattggtt ttctccttgt cttcatttcg aaacaaaagg gttgtgacaa
     5881 aatttttgtc acaacctctc cagcaaatag tgttctcctg ctaactcctc agtaataagc
     5941 tcaatggacc aggagctatt ttttgtttgg ctgtttttat cacttggtgc agggcagctt
     6001 tttcacgacc tctccactaa acagtattca aactgctgct tctcggtaat aagcccaacc
     6061 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tatgacttga tgcaggacag
     6121 ctttttcaca acctctcctt acattttaat ggagccgatc actttaccag ctgtcactgt
     6181 acctaattgc ttgatcacta cttcttctac tttatcgttt gtaaatacga tcatgatcgt
     6241 tggatctttt tcttcgtttt ggatcacatc aagatccatc tcagctaatt tcgttcctgt
     6301 tcctacttct tggccttctt tgacagcaat ctcaaaagct ggctgattca tttggactgt
     6361 atcgattccc atatggatca aaacttcgat accatctttc gtttccagcc caattgcatg
     6421 tttgctaggg aaaatacttg tcacttttcc agcgatcggc gcaacaactt ctcttgatgt
     6481 tggaataaca gcaaagccgt cacccatcat tttttgtgag aataccggat cttttacttc
     6541 agataaagga atcacttctc cagaagctgc ggaaactagt tcttgttcta tacctaagta
     6601 ttgcttacta cctgcttgtc catttcctgt agaaaccatt tctggctctg gaatctctgc
     6661 tcctgattgc agcagatctt cgatatctga tttcaagaca tctgcttttg gtccataaac
     6721 tgcttggact ccattgtctt tgacgatcaa tcccatcgcc cctgctcgtt tccaggcttc
     6781 ttcagtccct actttttctc gatcatttac acttacgcgt aaccgagtca tacaagcatc
     6841 gacatctttg atattttctt ttcctcccag caaatgaatg atctggacca cttgctgatc
     6901 tgctgttcct gttccgctgt tagatacaac ggtttcttca ctgtcattgt cgtaatttcc
     6961 gttacgtccc ggtgtagcaa agttgaattt tttgatcaag aaatttgcta agaaatacgt
     7021 aacaacacca aaagcgatca cacacaacac gaagttgata agatctccac ctagacccgc
     7081 tttgatagct aacggtgtcc gagtcagcaa ttcgatattc ccaaatgaat ggacccgcaa
     7141 tggcaagata tctgccatcg caaatgcagc cccttggatc actgaataaa tgacatataa
     7201 tggcacagca gcaaacataa acatgaactc caatggttct gttacacctg ttagaaagac
     7261 agccaacgct gctgaaaaat acattgattt atatcttgct ttcttatcag gatcgacatt
     7321 acgatacatc gcaaaagcca ttcccatcaa gatacctgaa gaaccaatca tttgtcctac
     7381 tttgaaacga gccggcgtcc agttatgcaa cacaaactca tattgcgaca tatcccctgc
     7441 acctttaaga ttcactaaat cagtggccca agccaaccat aacggatctt gcccaaatac
     7501 ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat ccgccaagct gtgtatagtt
     7561 gataggaatc gtcagcatat gatgcaatcc aaatggcaat agtaaacgtt ctaatgttcc
     7621 aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat tctgcaatcc ataaaccaaa
     7681 attattgatt ccagcctgta cgtttggcca caccacagca agaatcaatg ccacgattgt
     7741 tgaccataag ataacaacaa acggaacaaa gcgctttcca ttgaaaaatg acaaagcatc
     7801 cggcaatttt ctataattat agtacttatt ataagccatt gccccgacaa atccagcgat
     7861 gatccctacg aatactccca tattcaaagc tggtgcttcc aatacacttg taaaaaatcc
     7921 tttaaccatg atcttcgtac cgaacagagt gtgggtaaat gcatcttcat tcgcaagcat
     7981 gtcacttgtg acaccaaaga tagccccagt gatccgattg attaaaataa atgagatccc
     8041 agctgcaaat gctccgcctg ctctttcttt tgcccaactt cctcctattg ctaaagcaaa
     8101 taaaatatga agattaccaa taattgccca accaatattt tcaattactc cgcccgttgt
     8161 gacaagcggc gtccaatcag gattgatcaa gggaattgat ttccctatac tgatcatcaa
     8221 cccagctgcc ggcataactg ctaccacaac cattaaagcc tttccaaatt tttgccaaaa
     8281 ctcaaaacta aatatctttt tcattccgtt tagctccttt acgcaatcgg tttcgttaaa
     8341 cgtattatag cgtgcgtttt cttttttgta aatgctttat tttttatttt tctgtctata
     8401 ccattctata aaacctataa tacgtgtgtt cccttctata attttctcct aaaaatttat
     8461 ttttttaagc ttttttctat gaaatatttt cttttgtcta ttgacttgtg caatcggttg
     8521 cgtttattat gaacgtataa aacagctcat taggaggaga aaaatgaaac agatcaaacg
     8581 actatttgct attgatccat ggaaaatcag cacgacacaa ttagataaaa aaaatcttcg
     8641 actgcaagaa tctttaacaa gtattggaaa cggttatatg gggatgcgcg ggaactttga
     8701 agaaacttac tcaggggacc accatcaagg tacttatttg gcgggtgtct ggtatcccga
     8761 taagacacgt gtcggctggt ggaaaaatgg ttatccagaa tactttggaa aagtgatcaa
     8821 cgccttgaac tttatcgcga tagatatttc gatcaacgat cagctgatcg acttggcttc
     8881 tttggaacca gaagactttt actgggaatt agatatgaaa aatggtgtat tatctcgtac
     8941 attcacaatc actacttcta ctaataaagt acgcatttcc ttcgaacgtt ttctgagcat
     9001 tgtcaaaaaa gaatcagctt atatctattt aactatagaa atgcttgaag gtagcggaac
     9061 agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac gaagacagca actatgatga
     9121 acatttttgg aatgagattg atcgtggaac tatagaagaa atcggctatt tgacgactaa
     9181 aactattcca aacaactttg ggattgaaca atttactgtt actgcagcaa tgcgccattt
     9241 cgttaatgga aaatcagtca tccctgacta tcacgaagat actttagcat tacatggttg
     9301 cttgtctgca gaattatctc aaggtgagac atggtcagtg gaaaaagaaa ttatcgttct
     9361 tactagtaga gatattccag aagcgcagca agcagaaaaa gctgtctcag aattaacgaa
     9421 ccagacaaac aattatatcc aagcaaaaga agagcaaaca gcggcttggg cagaacgatg
     9481 gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca caacaaggta ttcgattcaa
     9541 tttgttccaa ctattctcta cttactacgg cgaagatgaa cgattgaata tcggccccaa
     9601 aggattcaca ggagaaaaat acggcggagc cacttactgg gatacagaag cgtatgctgt
     9661 ccctctttat cttgctttag ccaaacctga agtaactaag aatctgctga aatatcggca
     9721 taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa ggattaaaag gcgcgcttta
     9781 tcctatggtc accttcactg gtgtcgaatg ccataacgag tgggaaatca cattcgagga
     9841 gattcaccga aatggcgcga ttgcctatgc aatctacaat tatacgaatt acaccggcga
     9901 tacggaatat ttgaaaaatg aaggactgga agttttagca gaaatcgcac gcttctgggc
     9961 agaccgtgtc catttttcaa aacgagcaaa taaatacatg atccacggtg tgaccggccc
    10021 aaacgaatat gagaacaata tcaataacaa ttggtataca aatacgatcg cagcttgggt
    10081 cttgcgttac acgcgagaaa attatttaac ttatcaaaaa gaagctgcta tcactatcag
    10141 cgaagaagaa ctagccaaat ggaaagatat cgcagagaac atgtacttcc cagttgacga
    10201 agaattagga atctttgtcc agcatgacac attcttagat aaagatctca tgcctgtatc
    10261 cgatattccc acaagcgatc ttcctctgaa tcagcattgg tcttgggaca aaatccttag
    10321 atcttgcttc atcaagcagg cagatgtact acaggggatt tatttcttca atgatacttt
    10381 tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa ccaatgactg tccatgagtc
    10441 ttccctttcg ccaagtatcc attcgatttt agcagcagaa ttaggaatgg aaagcaaagc
    10501 tgtagaaatg taccagcgaa cagctcgctt ggatttggat aattacaata acgacacaga
    10561 agacggcttg cacatcactt ccatgactgg tagctggttg tcgattgtcc aaggatttgc
    10621 tcaaatgaaa actgcccatg atcagttgag attcgcacca tttctgccaa aaacttggaa
    10681 agcttattct ttccatatca attatcgcgg acgtttattg ttcatctcag tcacagatga
    10741 acaagttact ttcgagttat tagaaggttc tcctctagaa atttacgtct atgaacagtc
    10801 ttattcttta gcggatcgtc tagaaataac gctaagaaag gaggaagcaa atgtttaaag
    10861 gggtattgtt tgatttagac ggtgtcatta ccgatactgc tgaattccat tatcgtgctt
    10921 ggcgaaagct tggtcaggaa atcggcatta cgatcgacca cacattcaac gaacagctaa
    10981 aaggcgtcag ccgagaagac tcactctctt tgcttttagc ttatggcgga aaagaacact
    11041 ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa aaacgattat tatttagaga
    11101 tgatccagac gatcgaacca aaagatgtct tccccggggt cgtgccttta ctggattctt
    11161 taaaagaaga aaaaatcaag attgctcttg cttctgcaag taaaaacggg ccattcctac
    11221 ttgaaaaaat gggtcttaca ccttactttg atgctattgc cgacccggca gaagcaacaa
    11281 acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa agctgtcgga ctaaaagcag
    11341 aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca agccatcctc tcaagtggcg
    11401 ctcaaccaat tggcgtggga cgtgctgaag accttggaga ggagatcccg cttgtccctg
    11461 atacgacttt tcttacgatc gattacttga aagaaaagtg gcatgaccat ggataatcca
    11521 gagatctccg tcgtaccagt tccagagact gaaataaaga aaatcacatt gaagaacgac
    11581 tacttagaag tcgttcttct caattacggt gcccgcctgc accaaatctt cgcaccagac
    11641 aaagaaggaa aatcagaaaa tgttttattg tcttacgatc gtttcgaaga tgttctaaca
    11701 gacaaaagct tttttggagc aactgtcgga cctgtagccg gaaggatccg ggatgcttct
    11761 tggggtgagc atagattgga gaaaaactgc ggatcacacc atatccacgg tggcacgaaa
    11821 ggctggtctt tccaattttg ggaagtagaa gttttcaaaa ctccatcatc aataggtgtg
    11881 gtcttttact taaaagatga attttctacc tatccaggac caatcactgc tacgatcact
    11941 taccgtttaa caaaaaacga attagagatg acaacctctg ctagtagcct tgttgaaacc
    12001 atctgcaatc cgaccaacca tgcctacttc aacctttcag gaaatggaac aagagacatc
    12061 tatgaacatc agttaacggt ctttttagat ggtgtgcttg aattagatca agaaaaactg
    12121 cctactggaa attggacaaa aaaagaaaaa ctgccaatcg actttagaaa atccccaaca
    12181 ctgcaagaga tccttgcatg ttatccagat ggactagatg atgtatttct tttgcatcat
    12241 cctaggcttt ccaaaacttc cttacaatta ttcgagaagc attctggtcg ccaaatgact
    12301 atagcaactt ctaataaaag tatggtatta ttttctacga ctggattcga agctgatttc
    12361 tctgtcaatg gaaaacagat gcattcaaac tacggattag caatcgaacc gcaagaaatc
    12421 cctgatatcg ttcatttccc caaatttgga tcgatcaacc tccatcctgg tcaagaacgg
    12481 atcagtcaga cgatttatcg gttctctgca caataacaag agaaaggtgt ttttatcatg
    12541 gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg ccacctcgac cgtctctcgt
    12601 gtcatcaacg atcatcctag tatttccgag acaacaaaga agaaagtccg aaaaattatg
    12661 gatgaactag gttatgttcc taacatgact gcccgcaatc taggaaaacg catttccagt
    12721 gccatcggcg taatcttacc ccctcttgat tcaaaagagc ggatcggtaa cccattttat
    12781 cttgagatca tggaagcaat caacgaagaa gcgcggaatt atgatatgac tacagcaatc
    12841 gctactgcta aaaattttga attgctgtta gagaacgtcc agcgtatgca tttacaaaaa
    12901 caagtagatg gttttattct agtgtattca gatagtcaag atactgtcat tgattattta
    12961 taccaaaacc acatcccttt cactttgatc ggacagcctt atcatcatga gaacgaaatc
    13021 gtttatgtag ataatgataa ccagcttcta ggtaaacaag caacagaatt tttgatccag
    13081 aatggacata agaatatcct gtttgtgaca aacacgactc atgaaagttt gtatttcgaa
    13141 cgatatttcg gttatcaaaa agcaatgatg atggctggtc ttcctgcgtt atccagtgta
    13201 gtactagagc atccggagga ttatctgtcc tttgagaaaa cgttgaaaga aaacgaagcc
    13261 acagcattgg tggtcattga cgatatcttc gcccttcgca cgatgcagtt ggcgcaaatg
    13321 tatggctatg tcattccaga gacactctcc gtcattagct tcaacaactc gatcttctca
    13381 acattaacac acccttacct tacaagtatc gatatcgata tctccgaatt aggacgtgtc
    13441 gctatgcaaa agctgcgcga attgatccat gaagaattga gcagtggcgt acgtttggtc
    13501 atccctcatc gcttgatcaa aagagagaca gtcgttcctt tagaaaaccc atactaaata
    13561 gctgactcac taaatacgct gaccatttat cctatggtca gctttttatg taaaagaaga
    13621 tactacttct aacacattgt ttatctgtaa aaaagagcgt gggacaaaag taaagaacac
    13681 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat
    13741 aagctgaaat tcacaaaaat ttaaaaagca attttcttga gtttcttctt atttcttgga
    13801 gttaaacgct tctgtcccag cctctttttt ccttccttca attttttaca cgtcgacgat
    13861 tcaataaaaa accgcctaat attgtaaaga caggtgcaag aatcgagaaa aaggcgaatg
    13921 gcaagtaatg caacgcgcta actttcatcg ttcccataat gaatgtgccg ctgactcccc
    13981 acggtacgag agaattgacg gtcgcacctg aatccgctag tgttcgtgtc aaataggttt
    14041 ttgataagcc aacgtcatca aaagaacgct tgaatgtttc tcctggaaga atgatggaca
    14101 gatactgttc tccaacgatc agattgattc cgatacagct caaggcagta aacccgacta
    14161 aacgagaagg tcggtccatc ttttctttta attcttgaac gatggtatca acgatcagat
    14221 atttgatcag taacccacct aatgccaatg caagtaaaat caaactagca gaacctaaca
    14281 tactcatgat cccgcctcga gaaagcagtt catctagctt tggatcaggt gtatttgcga
    14341 cataaccatt catcaagatg tctgctgctt ttccgaccgt caaacttttc tcatggataa
    14401 aagaaagaac gagagcagct gtcgatcccg ctagtaatgt agggatagcc ggaatttttt
    14461 tgattgcccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag atccaaaagc
    14521 tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa tgactgttat
    14581 gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc gtataacgca
    14641 taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag ttggtcgtat
    14701 ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg cctgtagtca
    14761 ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca atcgtcgaaa
    14821 tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga aatgctgtcg
    14881 gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta gggatggttc
    14941 cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg attggaatga
    15001 tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg aactttttaa
    15061 atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt ggcgagagct
    15121 ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca gcgatacttt
    15181 cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa aggccctgcc
    15241 atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa cgtcggctga
    15301 aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc caagaatctt
    15361 tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata gctgctacta
    15421 ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg atgatagaga
    15481 agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca attcggtaag
    15541 gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt
    15601 acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca gcacttacgt
    15661 catccattac accataaagc aaagcaaata atgaaataac gattgattgt gttagaacga
    15721 catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt ggaaggaatc
    15781 cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct gataattgta
    15841 aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct ggtaagccta
    15901 ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt tttccatctg
    15961 gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat ccgatcaaag
    16021 cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc atgtaaacag
    16081 aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc aatgaatcaa
    16141 tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag ccgtttgaga
    16201 ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact actgggatat
    16261 atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat ttcaagttca
    16321 agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag tgattattgc
    16381 caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa gcgaccatta
    16441 ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc catttgctgc
    16501 ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc gtggaaagct
    16561 caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa
    16621 tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca cacagtgcca
    16681 ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta ctcaccgttg
    16741 acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt cagcaataaa
    16801 cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga agggtttctt
    16861 caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc tcttcagatc
    16921 caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt accttgctta
    16981 ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt cttgaagagc
    17041 tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt atggtgtcat
    17101 tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat ctgagaatcc
    17161 tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa agtcagtatg
    17221 tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat aatcatatac
    17281 gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt tgaatacata
    17341 gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt tgtctcctac
    17401 tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga ttgatgcacc
    17461 cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg cccaaacgct
    17521 ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta atgctgggat
    17581 atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc tcatgcggat
    17641 atctttgatg acgataccac cggcagaata tggaacatag cccattttat gtgggtcaat
    17701 cgttacagat tctgcttctt cgattgcttt atatgcactg tgtacttctt ctaagatata
    17761 atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat cttcaaatgg
    17821 aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat aagcagcatc
    17881 tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg caacgatttt
    17941 atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta cacctaggat
    18001 tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga tgtccatgcg
    18061 atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga tgatgtcggc
    18121 agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta atttttctaa
    18181 atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg ggacgctgtc
    18241 taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc tttttccagc
    18301 aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt tgcgggcata
    18361 ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc agccatcttt
    18421 atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc ttgttgcagg
    18481 agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt acgctagtaa
    18541 agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat tatgccaagg
    18601 aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg ttttattcac
    18661 agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca tgtcttgagg
    18721 catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca aagctttata
    18781 gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta gatctttcga
    18841 caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt cgagccctac
    18901 attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa tcatatttta
    18961 atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa acgagggaat
    19021 ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt ctattttagg
    19081 atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta aaaacttact
    19141 aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg aataaccaca
    19201 aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt aacgcggtca
    19261 ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt agatggttca
    19321 atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc
    19381 ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat atttccagag
    19441 aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg cacaaatctt
    19501 gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt ctctactttt
    19561 ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa gtatttcact
    19621 acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga agttttcttc
    19681 ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc agccttcagc
    19741 atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt tcggattagg
    19801 tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa ttgcacatcg
    19861 tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt aaactctgta
    19921 aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat cgtgttgatg
    19981 ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga caaccagtcg
    20041 tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat ttgattggaa
    20101 agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc tgttttgcgg
    20161 ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg atgatggcct
    20221 gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg catcgaatca
    20281 ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt
    20341 ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc aataatattc
    20401 attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc atatgtaatt
    20461 aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat aagccaaggg
    20521 acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac ctctcgaaca
    20581 tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt cgctttaaag
    20641 catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac aactacttcg
    20701 tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa ctttttcttt
    20761 gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg aatccgctct
    20821 aattccgaac attttatttt caattattgt taaaagaaat aactttttct gtattttttc
    20881 tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt acaataagga
    20941 atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa
    21001 ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt ttgttttttg
    21061 tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag
    21121 caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa
    21181 gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg
    21241 aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg
    21301 atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct
    21361 tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc
    21421 gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc
    21481 aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac
    21541 cattggttct ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc
    21601 ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata
    21661 aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc
    21721 ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt
    21781 taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta
    21841 aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata
    21901 catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat
    21961 tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg
    22021 gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga
    22081 agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat
    22141 taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg
    22201 atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca
    22261 aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac
    22321 attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg attcccttga
    22381 tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc tccggcttag
    22441 aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat
    22501 ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg aaactttctt
    22561 gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta tagtatgtca
    22621 tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg ttttcactca
    22681 tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc gcttcttgcc
    22741 ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg ctgccaaaat
    22801 atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc attgtcactc
    22861 cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt
    22921 tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat t
//
LOCUS       DAZUKA010000035        22830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000035 DAZUKA010000000
VERSION     DAZUKA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.035"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG8PN_001046"
     CDS             3..371
                     /locus_tag="ACG8PN_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4861215.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG8PN_001047"
     CDS             551..1492
                     /locus_tag="ACG8PN_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4861216.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG8PN_001048"
     CDS             1561..2292
                     /locus_tag="ACG8PN_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4861217.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG8PN_001049"
     CDS             2436..4616
                     /locus_tag="ACG8PN_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4861218.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG8PN_001050"
     CDS             4636..5118
                     /locus_tag="ACG8PN_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4861219.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG8PN_001051"
     CDS             5134..5895
                     /locus_tag="ACG8PN_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4861220.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG8PN_001052"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG8PN_001052"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4861221.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG8PN_001053"
     CDS             7667..8152
                     /locus_tag="ACG8PN_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4861222.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG8PN_001054"
     CDS             8145..8828
                     /locus_tag="ACG8PN_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4861223.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG8PN_001055"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG8PN_001055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4861224.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8PN_001056"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8PN_001056"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4861225.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8PN_001057"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8PN_001057"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4861226.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG8PN_001058"
     CDS             13125..13457
                     /locus_tag="ACG8PN_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4861227.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8PN_001059"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8PN_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4861228.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG8PN_001060"
     CDS             14283..15656
                     /locus_tag="ACG8PN_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4861229.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8PN_001061"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8PN_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4861230.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG8PN_001062"
     CDS             16711..17151
                     /locus_tag="ACG8PN_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4861231.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG8PN_001063"
     CDS             17295..17996
                     /locus_tag="ACG8PN_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4861232.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG8PN_001064"
     CDS             17993..19114
                     /locus_tag="ACG8PN_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4861233.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8PN_001065"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8PN_001065"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4861234.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG8PN_001066"
     CDS             20493..21401
                     /locus_tag="ACG8PN_001066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4861235.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG8PN_001067"
     CDS             21436..21708
                     /locus_tag="ACG8PN_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4861236.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG8PN_001068"
     CDS             21719..22765
                     /locus_tag="ACG8PN_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4861237.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7698 a         3982 c         5011 g         6139 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac
//
LOCUS       DAZUKA010000036        22727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000036 DAZUKA010000000
VERSION     DAZUKA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.036"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8PN_001069"
     CDS             complement(132..509)
                     /locus_tag="ACG8PN_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861238.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8PN_001070"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8PN_001070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4861239.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8PN_001071"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8PN_001071"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4861240.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8PN_001072"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8PN_001072"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4861241.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8PN_001073"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8PN_001073"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4861242.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8PN_001074"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8PN_001074"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4861243.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8PN_001075"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8PN_001075"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4861244.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8PN_001076"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8PN_001076"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4861245.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8PN_001077"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8PN_001077"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4861246.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8PN_001078"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8PN_001078"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4861247.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8PN_001079"
     CDS             10661..11575
                     /locus_tag="ACG8PN_001079"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4861248.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQEIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8PN_001080"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8PN_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861249.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8PN_001081"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8PN_001081"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4861250.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8PN_001082"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8PN_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4861251.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8PN_001083"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8PN_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4861252.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8PN_001084"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8PN_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4861253.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8PN_001085"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8PN_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4861254.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8PN_001086"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8PN_001086"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4861255.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..>22727
                     /locus_tag="ACG8PN_001087"
     CDS             21340..>22727
                     /locus_tag="ACG8PN_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4861256.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYS"
BASE COUNT         7313 a         3838 c         5149 g         6427 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagagat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattc
//
LOCUS       DAZUKA010000037        22639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000037 DAZUKA010000000
VERSION     DAZUKA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.037"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..3298
                     /locus_tag="ACG8PN_001088"
     CDS             20..3298
                     /locus_tag="ACG8PN_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4861257.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3839..5746)
                     /locus_tag="ACG8PN_001089"
     CDS             complement(3839..5746)
                     /locus_tag="ACG8PN_001089"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4861258.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5876..6751
                     /locus_tag="ACG8PN_001090"
     CDS             5876..6751
                     /locus_tag="ACG8PN_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4861259.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6831..7805
                     /locus_tag="ACG8PN_001091"
     CDS             6831..7805
                     /locus_tag="ACG8PN_001091"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4861260.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7985..9130
                     /locus_tag="ACG8PN_001092"
     CDS             7985..9130
                     /locus_tag="ACG8PN_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4861261.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9267..9611
                     /locus_tag="ACG8PN_001093"
     CDS             9267..9611
                     /locus_tag="ACG8PN_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4861262.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9882..11168
                     /gene="efmA"
                     /locus_tag="ACG8PN_001094"
     CDS             9882..11168
                     /gene="efmA"
                     /locus_tag="ACG8PN_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4861263.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11306..11905)
                     /locus_tag="ACG8PN_001095"
     CDS             complement(11306..11905)
                     /locus_tag="ACG8PN_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4861264.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12309..13430
                     /gene="mnmA"
                     /locus_tag="ACG8PN_001096"
     CDS             12309..13430
                     /gene="mnmA"
                     /locus_tag="ACG8PN_001096"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4861265.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13676..15097
                     /gene="arcD"
                     /locus_tag="ACG8PN_001097"
     CDS             13676..15097
                     /gene="arcD"
                     /locus_tag="ACG8PN_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4861266.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15111..16229
                     /locus_tag="ACG8PN_001098"
     CDS             15111..16229
                     /locus_tag="ACG8PN_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4861267.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16289..17581)
                     /locus_tag="ACG8PN_001099"
     CDS             complement(16289..17581)
                     /locus_tag="ACG8PN_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4861268.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17731..18291
                     /locus_tag="ACG8PN_001100"
     CDS             17731..18291
                     /locus_tag="ACG8PN_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4861269.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18420..18797
                     /locus_tag="ACG8PN_001101"
     CDS             18420..18797
                     /locus_tag="ACG8PN_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4861270.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18730..20802
                     /locus_tag="ACG8PN_001102"
     CDS             18730..20802
                     /locus_tag="ACG8PN_001102"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4861271.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21013..21960)
                     /locus_tag="ACG8PN_001103"
     CDS             complement(21013..21960)
                     /locus_tag="ACG8PN_001103"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4861272.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22206..>22639
                     /locus_tag="ACG8PN_001104"
     CDS             22206..>22639
                     /locus_tag="ACG8PN_001104"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4861273.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7327 a         4138 c         4808 g         6366 t
ORIGIN      
        1 tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa
       61 tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca
      121 acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa caatggagga
      181 aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta tgatgaaaga
      241 atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt
      301 tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag aagacggcgt
      361 gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag atggaatgac
      421 ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga cgatcgaaat
      481 agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa gagacgcagt
      541 accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca tactaggcag
      601 tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga gcggcagcaa
      661 atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat ctgaagatcc
      721 gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag agacttcagc
      781 agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc atgggaatct
      841 gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa cgaatactgg
      901 tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg acatcatgta
      961 tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat ttgctgcagc
     1021 aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc ctgcgacaga
     1081 tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg tgacctctag
     1141 taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg acggattatt
     1201 gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact tggctatcac
     1261 tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg aatttacttt
     1321 cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga ctagttatga
     1381 aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga cagtttatgt
     1441 aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat ctctgacaca
     1501 agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa cttattcctt
     1561 cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc cgattcagtt
     1621 gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag gaactggaaa
     1681 tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta caaatagtgc
     1741 agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag agattcatgc
     1801 ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc
     1861 atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg atccaggatt
     1921 tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg tatttgacgg
     1981 ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa atgaaacagg
     2041 ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct gggtaccagg
     2101 aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt taacgggccc
     2161 tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg attccaatag
     2221 cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat ataatgtggc
     2281 tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct tatctttccg
     2341 aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg aagatcaaat
     2401 ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa atactcttgg
     2461 acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag aaggtcagca
     2521 gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc agattaaacc
     2581 ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg taggtgctat
     2641 ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg tacaagaacc
     2701 aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag caaatggtgt
     2761 ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt ttgtgtcaac
     2821 tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc cagaaaagat
     2881 ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta ttttttcagc
     2941 accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat ttgatgggga
     3001 aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac
     3061 tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa atgaatcacc
     3121 aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag aaagtgaaaa
     3181 aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc tagcattagc
     3241 tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt
     3301 ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat agaaggaaat
     3361 gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct ccttgctgct
     3421 tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct ctccactaaa
     3481 cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta
     3541 tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac ctctcgataa
     3601 acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc
     3661 cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca acctctaata
     3721 catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa aaatttgaga
     3781 agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc acaacctctt
     3841 accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa
     3901 atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg
     3961 catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa
     4021 tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt tttgctgctt
     4081 ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt
     4141 ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt
     4201 tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa
     4261 ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc
     4321 caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg
     4381 ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca
     4441 tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga
     4501 gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt
     4561 tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat
     4621 ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac
     4681 gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt
     4741 cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa
     4801 ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat
     4861 agattccgct gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt
     4921 taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat
     4981 gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg
     5041 tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca aaagattgcg
     5101 gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca
     5161 atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg
     5221 caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg
     5281 gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga
     5341 aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta
     5401 atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac tgcaatgatt
     5461 caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt cggtcataat
     5521 cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg
     5581 gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat
     5641 cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt
     5701 cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc
     5761 ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa
     5821 aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa
     5881 aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga
     5941 taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct
     6001 ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc
     6061 ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa
     6121 gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc
     6181 cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc
     6241 agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac
     6301 ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc
     6361 aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag
     6421 ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag
     6481 ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca
     6541 gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact
     6601 tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg
     6661 agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag
     6721 ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg
     6781 gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata
     6841 agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa
     6901 aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg
     6961 gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat
     7021 caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc gatgcaatga
     7081 agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag
     7141 accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag
     7201 aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct
     7261 ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat
     7321 taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg
     7381 cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg
     7441 cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag
     7501 atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg
     7561 gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta
     7621 ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa
     7681 atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt gaagcggtca
     7741 aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg
     7801 aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc aaggagctgc
     7861 gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc tagacaaaaa
     7921 tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca ataatttgga
     7981 agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc ccatgcatcc
     8041 acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc cttcaagcat
     8101 acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga
     8161 cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag aaggggataa
     8221 tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc taatcactac
     8281 agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa aggggtttga
     8341 cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc
     8401 gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa tcggaaattt
     8461 gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt tccatacaga
     8521 tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa tcgatttatt
     8581 aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga
     8641 cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg
     8701 tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc tgtcacctga
     8761 agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc ttgatacatt
     8821 agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac tccctcatgt
     8881 gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa
     8941 tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc cttcacacgt
     9001 attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc gtatcagctt
     9061 tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag ttcgtgctgt
     9121 caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa cgccttcatt
     9181 ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta taataaagag
     9241 aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg gtactgggcg
     9301 caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc gataatggat
     9361 ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc acgccgcaaa
     9421 gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt
     9481 cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca aaacagaaaa
     9541 tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg ttattcgaaa
     9601 aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct attgttagat
     9661 gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa tttaacacat
     9721 gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt taaattgaaa
     9781 cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata atgattgttg
     9841 tgcaaacgct atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg
     9901 tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag
     9961 gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt
    10021 cggcgacaat tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc
    10081 catttgtagg accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata
    10141 ttattgtagc gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc
    10201 cgatttggct agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc
    10261 caacgatcca gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg
    10321 gacagttagg aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt
    10381 tgttttcagt cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg
    10441 gtgtcggctt attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc
    10501 cgcttactat ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt
    10561 tatggtacat aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc
    10621 tgtatccatt gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg
    10681 aagtagtcta cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat
    10741 ggaaagaccg aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg
    10801 caagcggatt ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag
    10861 gagcaggttg tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt
    10921 atgaatcgaa tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag
    10981 gtccaattgg attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt
    11041 tcttgttttc cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg
    11101 cccgaaatta tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag
    11161 acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta
    11221 atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt
    11281 taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga
    11341 tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt
    11401 ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat
    11461 ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag
    11521 tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt
    11581 tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac
    11641 tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa
    11701 tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat
    11761 ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca
    11821 catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt
    11881 gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca
    11941 aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc
    12001 agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc
    12061 cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca
    12121 aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac
    12181 agtattttta cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta
    12241 aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg
    12301 gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg
    12361 actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca
    12421 tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg
    12481 atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag
    12541 aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta
    12601 acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg
    12661 atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg
    12721 gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc
    12781 aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag
    12841 aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg
    12901 gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa
    12961 aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt
    13021 attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac
    13081 cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc
    13141 atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg
    13201 aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc
    13261 ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac
    13321 gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg
    13381 gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa
    13441 atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg
    13501 tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt
    13561 tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat
    13621 agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga
    13681 aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat
    13741 cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc
    13801 gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca
    13861 tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt
    13921 tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa
    13981 tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg
    14041 tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt
    14101 agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt
    14161 agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac
    14221 tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac
    14281 gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga
    14341 aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat
    14401 cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt
    14461 cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg
    14521 aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg
    14581 ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg
    14641 tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt
    14701 tgttactgca atcttgacta atctattctt attgactttc ttagttactg attatgccta
    14761 tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat
    14821 ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat
    14881 tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg
    14941 tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca
    15001 tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc
    15061 aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg
    15121 caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg
    15181 cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg
    15241 taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat
    15301 cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct
    15361 tacaaacgaa tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag
    15421 gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt
    15481 taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag
    15541 aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca
    15601 atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta
    15661 cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat
    15721 tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct
    15781 actccattcc aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt
    15841 ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg
    15901 tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga
    15961 accttgtgat tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca
    16021 attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta
    16081 tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga
    16141 agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct
    16201 tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa
    16261 ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag
    16321 ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat
    16381 tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc
    16441 gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg
    16501 cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg
    16561 tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta
    16621 aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa
    16681 aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc
    16741 ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg
    16801 taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg
    16861 aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat
    16921 atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa
    16981 atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa
    17041 tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa
    17101 gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt
    17161 ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt
    17221 gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga
    17281 cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta
    17341 attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc
    17401 cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca
    17461 taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa
    17521 acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca
    17581 attagactcc ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg
    17641 tagtttatca atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga
    17701 aggatttatt ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa
    17761 atcatcgact gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag
    17821 catttagcca aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt
    17881 cgtgatgtca aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga
    17941 cgggctcttg tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga
    18001 tcctttgctt tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat
    18061 gaagctgttt tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag
    18121 gcttttcaat taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc
    18181 cacggttatc tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca
    18241 gaagacagct atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga
    18301 ttcatttgta acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg
    18361 caactttctt gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta
    18421 tgtatcaagt aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg
    18481 aagaagatat tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg
    18541 aaaaacaatg gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc
    18601 tcagcgcctt ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac
    18661 agcaatacaa gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa
    18721 aggaatttta tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc
    18781 aaggtgcttg gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt
    18841 acttgggccc ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg
    18901 cagagctggc aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt
    18961 cctaaaacag gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca
    19021 gaagcagtca ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta
    19081 gagtatgtgc atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa
    19141 gcaatcaaaa ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa
    19201 tctgctcttg cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta
    19261 actgtgaagt acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta
    19321 gtgagagacc aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg
    19381 ttgcaagctg gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca
    19441 caagaagaag cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa
    19501 gtccaagaaa aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa
    19561 agagaagcca atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt
    19621 ttgtatgaga cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat
    19681 gacatgaaaa gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc
    19741 aagcagtatt tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa
    19801 gtgaccgatc accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt
    19861 aatgaagagc gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa
    19921 ccccataaag tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt
    19981 cggcaaaatc gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca
    20041 gtcaaatggg aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct
    20101 gcaccgccta aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg
    20161 ggaaccccag ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa
    20221 cgtacccctc aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat
    20281 ccctctttag taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct
    20341 gcaggaaaga aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg
    20401 gtgaatgaga tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa
    20461 tgcccagaat gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc
    20521 tgcaccaatt cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga
    20581 tgcccgcaat gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt
    20641 tgtaaatacg atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg
    20701 accaaacatg aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa
    20761 agcccattag cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt
    20821 gtgatagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat
    20881 ttttgtgaaa tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa
    20941 aaaaggactg tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc
    21001 atttgacgta tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct
    21061 tatgatataa acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat
    21121 gattccaaga cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg
    21181 ccatcctgat gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat
    21241 accagaaccg acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga
    21301 gaagttcacg ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc
    21361 gccagccaca cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct
    21421 aaataatcct tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg
    21481 acgagtcatc aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt
    21541 gatcaactgg tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt
    21601 cgaacgccgg gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg
    21661 aagcagttcg ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga
    21721 agcttgcaat ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa
    21781 gcggacataa ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga
    21841 tccgtcgtca ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc
    21901 aaaaaaaaga ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat
    21961 cgtttctatc ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct
    22021 taaataacac ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta
    22081 acgtatttag tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag
    22141 ttggcaagta gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa
    22201 acaaaatgaa aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat
    22261 tgactgctgt atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa
    22321 cgcgcacaca agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc
    22381 ctttatctat aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca
    22441 ttccttatgg agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag
    22501 ctgtgcgagc gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc
    22561 atcgagtgat cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg
    22621 ttgaaatcaa acgccaatt
//
LOCUS       DAZUKA010000038        22522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000038 DAZUKA010000000
VERSION     DAZUKA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.038"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..226)
                     /locus_tag="ACG8PN_001105"
     CDS             complement(29..226)
                     /locus_tag="ACG8PN_001105"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4861274.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(372..1250)
                     /locus_tag="ACG8PN_001106"
     CDS             complement(372..1250)
                     /locus_tag="ACG8PN_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4861275.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="ACG8PN_001107"
     CDS             complement(1289..2407)
                     /locus_tag="ACG8PN_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4861276.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="ACG8PN_001108"
     CDS             complement(2404..4503)
                     /locus_tag="ACG8PN_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4861277.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="ACG8PN_001109"
     CDS             complement(4554..5375)
                     /locus_tag="ACG8PN_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4861278.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="ACG8PN_001110"
     CDS             complement(5372..6181)
                     /locus_tag="ACG8PN_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4861279.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="ACG8PN_001111"
     CDS             complement(6202..6696)
                     /locus_tag="ACG8PN_001111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4861280.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7208)
                     /locus_tag="ACG8PN_001112"
     CDS             complement(6708..7208)
                     /locus_tag="ACG8PN_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4861281.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7273..9969)
                     /locus_tag="ACG8PN_001113"
                     /pseudo
     CDS             complement(7273..9969)
                     /locus_tag="ACG8PN_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(10397..11470)
                     /locus_tag="ACG8PN_001114"
     CDS             complement(10397..11470)
                     /locus_tag="ACG8PN_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4861282.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11467..12294)
                     /locus_tag="ACG8PN_001115"
     CDS             complement(11467..12294)
                     /locus_tag="ACG8PN_001115"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861283.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12295..13101)
                     /locus_tag="ACG8PN_001116"
     CDS             complement(12295..13101)
                     /locus_tag="ACG8PN_001116"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861284.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13113..14198)
                     /locus_tag="ACG8PN_001117"
     CDS             complement(13113..14198)
                     /locus_tag="ACG8PN_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861285.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14215..14757)
                     /locus_tag="ACG8PN_001118"
     CDS             complement(14215..14757)
                     /locus_tag="ACG8PN_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4861286.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15015..16004
                     /locus_tag="ACG8PN_001119"
     CDS             15015..16004
                     /locus_tag="ACG8PN_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4861287.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16001..16738
                     /locus_tag="ACG8PN_001120"
     CDS             16001..16738
                     /locus_tag="ACG8PN_001120"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4861288.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16735..17286
                     /gene="coaC"
                     /locus_tag="ACG8PN_001121"
     CDS             16735..17286
                     /gene="coaC"
                     /locus_tag="ACG8PN_001121"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4861289.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17302..17934
                     /locus_tag="ACG8PN_001122"
     CDS             17302..17934
                     /locus_tag="ACG8PN_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4861290.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18019..18666
                     /locus_tag="ACG8PN_001123"
     CDS             18019..18666
                     /locus_tag="ACG8PN_001123"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4861291.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMPEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18671..19654
                     /locus_tag="ACG8PN_001124"
     CDS             18671..19654
                     /locus_tag="ACG8PN_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4861292.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20057..21571)
                     /locus_tag="ACG8PN_001125"
     CDS             complement(20057..21571)
                     /locus_tag="ACG8PN_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4861293.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21709..22515
                     /locus_tag="ACG8PN_001126"
     CDS             21709..22515
                     /locus_tag="ACG8PN_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861294.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6799 a         4835 c         3905 g         6983 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctat ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10021 aataatttat ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta
    10081 caaatattga actggattca tatattagtt ttattaatag attacagaag ggttaagtat
    10141 atttgcatag tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag
    10201 cccgtctcgg gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg
    10261 tagaaaaaat aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg
    10321 aaaactcaga tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc
    10381 ttactgagtt ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga
    10441 tgcctaggta tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag
    10501 atggatagaa ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat
    10561 ttggtgtaga ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa
    10621 agttgatgaa atcataggca ccttctttat ttttcacggt ttttggcata acgatattat
    10681 caaaccaaag attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca
    10741 tcatatcggc agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat
    10801 acattttgat ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat
    10861 ttgctgcttc tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca
    10921 aagaaagccc cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg
    10981 gcttccacaa atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata
    11041 cgatccctaa agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca
    11101 agtctaaaaa tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa
    11161 gtactagttt ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt
    11221 agtttgtccc accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat
    11281 aattcacttt gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat
    11341 ctccccagtt atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt
    11401 gacgaacacc aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta
    11461 attttttcat cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt
    11521 tggacggttt tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa
    11581 aactaacgca cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat
    11641 ctctactgag agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc
    11701 aagagaatag gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa
    11761 aatgatattt ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat
    11821 tgaatcgttc atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa
    11881 agcaatatgg gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa
    11941 aatcaagaaa ctagcaccga taataacatc aggagagact aataagatgt tattcatgct
    12001 caacaacgct gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt
    12061 cccgatgatc gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat
    12121 aatgatcaaa cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa
    12181 gctgttcatc gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata
    12241 taggaataaa aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga
    12301 gcgacctcct tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa
    12361 aatcacaccg attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat
    12421 agccgttcct aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga
    12481 aagagaagga ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa
    12541 aatcactcgt cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa
    12601 agaaggattc atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat
    12661 atacgttgcc acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc
    12721 gataaaatca aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta
    12781 agctttaagc agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt
    12841 gtgcttcagt tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt
    12901 gatcaaaaat gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa
    12961 ataagataga taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat
    13021 gatcattaat acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata
    13081 gatcctgcgc attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca
    13141 tcgaattctt cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc
    13201 aatcctactt tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc
    13261 tgttcatcat aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt
    13321 acgactagtt ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact
    13381 tcaactggtt catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac
    13441 tcaactagat tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca
    13501 gcaacgaaat gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt
    13561 ttccctttat tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga
    13621 gtaacaaaaa taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata
    13681 tctgtacgta gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt
    13741 tcatttacaa ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta
    13801 atctcccgtt tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt
    13861 tcgatatctg atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca
    13921 ttcatatgag gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct
    13981 ggcaaatcat tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat
    14041 ccagctataa tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa
    14101 aattttcctt gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt
    14161 ttcactactt gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa
    14221 atatgattgc gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg
    14281 atgttgtttt gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc
    14341 tcctaagctt aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg
    14401 ttcaaacgtc ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt
    14461 ttcattcgaa tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac
    14521 cgtatgatct tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc
    14581 ttctggagtc acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact
    14641 taaatctcgt tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc
    14701 ttcttgtgtc aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat
    14761 ttcctccgaa tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta
    14821 tacgaaattt attgaaaaag gcaacccttt gtaaaatttt ttttgctgaa tatttcttta
    14881 tcttttggaa taaattctag ttgtttgatt ctttattttt cacctaattt ttgtctaaat
    14941 ttacggaaga acaagaataa aagagtaggc tttatggtat gataaaagaa aattttaaaa
    15001 ggagtgtctc acgaatgaac gcttttcgat cttttcaggt aataaaggaa cctgttttcc
    15061 atacagcaat cagtgatctg ccatttactg agttggctga aaatgaagtc ttggtaaaaa
    15121 ttgcctattc tgatgtcaat tataaagatg cattggcaat gagcgagtct ggtcaggtga
    15181 tacgaacata tccgatgact ccagggattg atttatccgg aactgtcgtc caaagtaaca
    15241 atccacgttt ttcaaaagaa gatcttgtac ttgctactgg ttttgggctt ggcgtcaccc
    15301 atccaggcgg ttatagccaa tatcaaaaag taccaggaga ctggttagtt cctctaccaa
    15361 aaaatatgac gctgcgtcaa gcgatgatcc ttggtacagc agggtttact gctatgttgt
    15421 gtgtgaacgc tttaatccgt caaggtatga cctccgataa aaaagttgtc gttactggtg
    15481 cttctggcgg tgtaggcagt acagcgatag ccctgttaca taaactagga ttccagtcaa
    15541 tcattgcctt ttccagaaaa gaagagtctg tcacatggct aaaaagtcta ggtgctttgc
    15601 aagtcgtacg acctgatgaa tttctgccag agacaacaaa agctttagga aaacagcaaa
    15661 tcgactatgt catcgacaca gtaggtggcg agcagctaag cagtcttctt ccattgatct
    15721 cttataacgg tgcagtagct ttatgtggca atgctggggg aatcaaacta aacgcgactg
    15781 tcttgccttt tattctgcga aacatccagt tgattggaat tgattctgtc aatgtaccaa
    15841 tagatcaacg tctttcttta tggcaacaga tggcggattt gcagattgca gatgaactgg
    15901 tcgtccaaga gattactctg gatcaattgc ctgaaacagc atctaaacta cttgctggaa
    15961 cacaccaagg aagaacacta gtaaatgtag gtgaccataa atgaagattc taatcacagc
    16021 tggaggaacg actgaaaaaa tcgatcaagt acgtgctatt acaaatcatt cgacaggacg
    16081 attagggcaa gcattagctg attatttagc tgccaatccg gataccacaa tcgattacgt
    16141 gactacgaaa caagcactga aaccgaaaag acactccaat ataacgattt atacgatcga
    16201 atctgcactg gacttatttt tacaattaga agaactaacg aaaaaagaac actacgatgc
    16261 gattatccac agtatggcag tcagtgattt tactcctgct tttagttttt cagaagaaca
    16321 gctggcaaaa aacctaccca cttcttcaac gcaagaagaa ctggacaatt ggtttgcgga
    16381 aaatgaacag acagatacca ctgtttcaaa gatctcttct aacaccgagc atcttgtgct
    16441 cgttttgaag aaaacaccaa aaatcatttc ttatctacgt gaatggcagc caaaagcaaa
    16501 aatcattggt ttcaaactat tagtagatgt accaaaggaa tctcttctgg cagtcgcaaa
    16561 aaatagtctg ataaataaca aaactgattt tattttcgca aatgatttga cggaaatcca
    16621 tggcgagaca caccacggct atctcttatc taaagatgga acagtggaag aagctcaaag
    16681 taaatctgag attgctgcat tgattactga gaaaattaga ctggaggaat caaaatgaaa
    16741 aagatcttac taggcgttac tggaagtatt tctgcttaca aaagcgctga tcttacaagt
    16801 caattcgtca aattaggtgc ccaggtagat gtgattatgt ctaaaagcag cacacatttt
    16861 atcacgccat taaccttgca atctctgtcg aagcgcgcag tccatacaga cgtcatgcaa
    16921 gaatcaaatc cgcaggtaat caatcatatc gaactagcaa aacaagctga cttgtttctc
    16981 atcgcgcctg ctacagcaaa cattatcggc aagctagcaa acggcattgc agacgatctt
    17041 ctttcaacag tcgcaatggc gcttccagtg ggaacaccaa aattgattgc ccctgccatg
    17101 aacacgaata tgtatcaaaa cccgatcact cagcgtaatc tggaaacatt gagatctgtc
    17161 ggctatcagg aaattgaacc aagagaatct cttttagctt gtggagattt tggaaaaggt
    17221 gccttagctg atattcagac gatcatcgaa caggtcatgc tgactcttaa tgaaagaaat
    17281 gcttagaaaa aaggagtact tatgaaaaat acaaaaaact ttacattaac tgcgatgttt
    17341 ttagctattt tgattttatt agctgttacg cctttaggtt ttatccctat cggtccaatc
    17401 aatgcaacaa ccatgcatat tcctgtaatc attgcttcga tcgttttagg accaagacta
    17461 ggtgcttttc ttggcggtac atttggactg atcagcatga tccgtagcac agttatccaa
    17521 acacctttgt cttttgtctt ttctccgttt atcccagtca tcggaacaga acatggaagc
    17581 tggaaagcct tgctgatcgc gtttattcct cgcatcttga ttggggtggt tccttatttc
    17641 gtttataaag gtctgcgcaa actaatgaaa caaaaagcag attccgtttc tttattctta
    17701 gctggacttt ccggttcatt gaccaatacg attttagtga tgaatatgat ctacttttta
    17761 ttccagcaag attatgccaa agtcatcgga acaaatttaa atgctgttta ttcagctatt
    17821 ctagcggtta tttttacaag tggtatacct gaaggaatcg tcgcaggtct tgcgactgca
    17881 gcagtatgca atgtcttgtt aagatacttc aagcaccctt ctcttactaa ctgatagtca
    17941 tcgtagccga tctctgattt ttttctcttt tggtgttact attgaatgga aagatttgac
    18001 gtcgaaagga tgatccaaat gaaaattttt gttgtaggtg caaacggaca aattggccga
    18061 cacctgataa aagatttagc tccaagttcc catgaaattt ttgcaggggt gagagatgtt
    18121 gcttcccaaa cattagtaaa aaaagagaat gtttcgtatg tctcttttga tttaacttgg
    18181 tccgtggaaa aaatgcccga agcattcaaa ggcatcgatg tattgatttt tgctgccggc
    18241 tctcaaggaa agaatcttct gcaagtagac ttggatggcg caatcaaaac agtgatcgca
    18301 gctgaaaatg cgcatgtctc tcgctatctg atggtcagtg ctgtttatgc agatgaacca
    18361 gcaaaatggc ctgagtccat gacagattat tatattacta aacattatgc agatgaatgg
    18421 ctgaaacgaa ctaacttaga ttttgtcatt cttcaaccag tcacattgac taacgatgaa
    18481 gaagttactt ccattcaatt aactaagcct aatgagaagg catcaaaaac gatcactcgc
    18541 tctacagttg ctgctgtact tgccgctcta gtggaagaaa cggatatttc tcgtacgact
    18601 ctagtacttt ctgaaggtag caaagagttg aatacagcat tccaagaatg ggcaaaggag
    18661 gaatagatcc atgcgtgcca tttcaatcaa gcatcctggt tctgtagatg tattagaaca
    18721 aattgaagtg cctgttccta aaccaaaaca aggcgaatta ctgatcaaag tccatacagc
    18781 agctgtcaat cgaacagata ttatgaaaag agaatcagtc tctttgaaac ctccctaccc
    18841 tattttggga gtcgaagtag ctggagaggt agttgagaat aaaagtgata tctccacttt
    18901 tactcctggg acaaaagtat acggattagt caatctaggt ggttatgccg aatatgctgt
    18961 gatgcctgcc gatcgtgcaa tccttctccc tgatacgcta gactatgttt ctgctgctgg
    19021 aatctcagaa gtttttttga ctgcttatca aacactttac tggcttggaa aattacaaga
    19081 aggcgaaact gtcttgatcc atgcaggagc aagtggtgtg ggaacagcag cgatccaatt
    19141 agctagacag ctttctcacg cgaaaatcat cgttactgca ggatcagaag aaaaactcgc
    19201 cttttgtaaa gaacttggtg cagatgaatt gatcaactat aaaaaacaag actttcaaga
    19261 agaggtacga aaaatcactg aaggtcgggg agtcgatgtt attttggatt tcatcggcgc
    19321 ttcttactgg gaaaagaatt tagcttctat agccgttgat ggacgttggg tcttgattgg
    19381 tatgctaggt gggaccatcg tgcctgagat agaccttggt gtattgattg ggaaacgtgt
    19441 ccaactgctt ggtacattat tgacacctag aagtgatcag tataaagcag agctcgcaca
    19501 aacttttatg caaaaagctg ggccttttct agaaaatggc aagattcggc caatcattga
    19561 ccgtacattc caattcagcg cagtcaaaca agcgcatgaa tatatggaag caaacaaaaa
    19621 catcggaaaa atcattttga agataactga ataaaatcat gctgatcaaa aaagaggctg
    19681 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
    19741 tttgtgaatt tcagcttatt tccgaaggaa tttcttctgc ttccgccgtt tatacgtgtt
    19801 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttttcc gaataaacgg
    19861 tgttcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagaagctgt
    19921 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttcactaa
    19981 acagtgttca atcagctgac cctcggtatt agctcaaaac gctgatttca caacctcttt
    20041 ttatttacgt tctatttcat tcattttccg aagcaaatcg agctgcttgt atgcccgctt
    20101 gtcgcccaaa aataatgatc tccgcaactg aatttccacc gatccggttg tttccatgta
    20161 agccacctgt gacttcacca gcagcatata accccctaat tggctggttt tctttatcta
    20221 atacttcagt gttcgtattg attttcacac cgcccatcgt gtaatggatt cctggtgcga
    20281 ttttgatcgc ataataattc ggagacgata agtcatgttc catggctgtg gcacggttga
    20341 attgagcatc ttttttctcc gcaacatccg tattccatcc atcaactgtt tttgctagtg
    20401 tttcttttgg taaatcgatt ttttctgcta attcttccat tgtctttcca gttgtgacat
    20461 atcctttttg gtcataaaat tcaatagctg tggcacgatc acgcacgcct tggtcaaaaa
    20521 tcaaataagc tgatttttca ggcaatgctg taattgctgc agatacttta tcccttgtat
    20581 ccatttcatt gacaaatctt ttcccctctt gatcgaccaa gattgctcct tctccgcgaa
    20641 caacctcacc aatcaagacc ccctcatcct gctgcacagt tggatgaatt tgtattttat
    20701 ccatatcaac ggtttgtcca cctaattttt caatcatggt gataccatct ccagttgatc
    20761 cttcttgatt tgtcgtaata taatcagcta aatcgggacg atatttttca ataaattctt
    20821 tgctggcacc aaaaccgcct gtggtaacta cgattgcttt tccggtgacc tcttttggtt
    20881 cttctccttg aacatgtaca gtcacttcat tgaccgtttc cccatttttc ttaatgtctg
    20941 tcacatctgc gttgacaaaa atagggattt cccgctcatg gacgttattc aataacccct
    21001 ccactaagta tccaccaata gccgagccat cagacggacg atgtgtcctc ttttcactca
    21061 tccctccagt gatcgttaag ttgtccagtt tgataccgtt ttgatctagc caatcgaccg
    21121 catcagcaga gtgatcgaca aagtatcgaa gcatttcttt atcattcgtt cctccaccac
    21181 cttttagcgt ttcttcatag aaggcatcat tcgagtcttt gattccttct tctttttgaa
    21241 attttgtttc tgaagcattc ataccgcttg atgatttaga tgtgtttcct ccggcaacag
    21301 gcatcttttc caaaattact ggattcaacc cagcatcttt tgcttcgata gctgctgcca
    21361 tccctgcacc accagctcca ataatcacga catcataaga ttctttcatt tcttttggat
    21421 ctgtatatac ttgttcagaa gcgcctgatg atacttcggc agattccttc gtactagaac
    21481 ttgtctgcgc tgtatttttc tgatctgcgc cgcatgcgct catagcaaga agtgcaacgg
    21541 ctgttaataa accagcaact aattttttca tttgtatccc ccttttctcc ttatactata
    21601 ttaagtgatt tttttcacaa tatcaactct gtttttcttt aagtcatata gctttggtaa
    21661 acacacgctt cttagtataa ttaaggatat acatgaagga gttattttat gaatcatttg
    21721 aaaacctttt ttacgaacag tacaaaaact gcaggtagaa tcacaaacgg aatctatatt
    21781 ttattacgta tcatcgtcgt tattatgcta gtggatcagg tctggaacca acactgggga
    21841 aatacaatgc ttttattact gacattaatt ttatttgaaa tcccttattt tctggaaaag
    21901 atattaaaga tacagattcc aaatatgcta gaaatcattt tgttatcttt tatttttagt
    21961 tcaacagttt taggagagtt aagtgatttt tatggctatt tttcaatgtg ggatactttt
    22021 ctacatgcat tcagcggatt tttagctggg ggtgtcggct tttcccttgt ttatttgctc
    22081 aataaaaata caaaaaccat gaacttgact cctttattac tagcagttgt ttccttctgt
    22141 ttttcgatga cagttggtgt tatgtgggag tttgtggaat tttcagcaga taaattactg
    22201 caattcgata tgcaaaaaga tacgatcatc cagcaaatca atagtgtgga gttaagtaaa
    22261 gacggaaaca ctgtctatca tgttccagat atcactcgga caatcattga aagtcaaaat
    22321 gcagatggta aaacagataa aacaatcatc caaaatggtt acttagacgt gggaatcatg
    22381 gatactatga aggatttatt tgttaatttt attggagcag ttgttttcag tgtatttggt
    22441 tacctctata gtcgaaacga ttaccaaaaa tttacgttca tcaagaattt tattcctaaa
    22501 aaaatcaaac gataaaacat gc
//
LOCUS       DAZUKA010000039        22186 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000039 DAZUKA010000000
VERSION     DAZUKA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.039"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..541
                     /locus_tag="ACG8PN_001127"
     CDS             20..541
                     /locus_tag="ACG8PN_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4861295.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            638..859
                     /locus_tag="ACG8PN_001128"
     CDS             638..859
                     /locus_tag="ACG8PN_001128"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4861296.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1016..1180)
                     /locus_tag="ACG8PN_001129"
     CDS             complement(1016..1180)
                     /locus_tag="ACG8PN_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFS4861297.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1283..1804
                     /locus_tag="ACG8PN_001130"
     CDS             1283..1804
                     /locus_tag="ACG8PN_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4861298.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1791..2231
                     /locus_tag="ACG8PN_001131"
                     /pseudo
     CDS             1791..2231
                     /locus_tag="ACG8PN_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2271..2429)
                     /locus_tag="ACG8PN_001132"
     CDS             complement(2271..2429)
                     /locus_tag="ACG8PN_001132"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFS4861299.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2522..3283
                     /locus_tag="ACG8PN_001133"
     CDS             2522..3283
                     /locus_tag="ACG8PN_001133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4861300.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3470..4822
                     /locus_tag="ACG8PN_001134"
                     /pseudo
     CDS             3470..4822
                     /locus_tag="ACG8PN_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4838..6058
                     /locus_tag="ACG8PN_001135"
     CDS             4838..6058
                     /locus_tag="ACG8PN_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4861301.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6093..6770
                     /locus_tag="ACG8PN_001136"
     CDS             6093..6770
                     /locus_tag="ACG8PN_001136"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4861302.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6767..7963
                     /locus_tag="ACG8PN_001137"
     CDS             6767..7963
                     /locus_tag="ACG8PN_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4861303.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7978..8301
                     /locus_tag="ACG8PN_001138"
     CDS             7978..8301
                     /locus_tag="ACG8PN_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4861304.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8305..8640
                     /locus_tag="ACG8PN_001139"
     CDS             8305..8640
                     /locus_tag="ACG8PN_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4861305.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8637..9032
                     /locus_tag="ACG8PN_001140"
     CDS             8637..9032
                     /locus_tag="ACG8PN_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4861306.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9029..9400
                     /locus_tag="ACG8PN_001141"
     CDS             9029..9400
                     /locus_tag="ACG8PN_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861307.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9416..9976
                     /locus_tag="ACG8PN_001142"
     CDS             9416..9976
                     /locus_tag="ACG8PN_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4861308.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10103..10414
                     /locus_tag="ACG8PN_001143"
     CDS             10103..10414
                     /locus_tag="ACG8PN_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861309.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10438..10629
                     /locus_tag="ACG8PN_001144"
     CDS             10438..10629
                     /locus_tag="ACG8PN_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861310.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10629..14474
                     /locus_tag="ACG8PN_001145"
     CDS             10629..14474
                     /locus_tag="ACG8PN_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4861311.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14475..15347
                     /locus_tag="ACG8PN_001146"
     CDS             14475..15347
                     /locus_tag="ACG8PN_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFS4861312.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15344..16393
                     /locus_tag="ACG8PN_001147"
     CDS             15344..16393
                     /locus_tag="ACG8PN_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861313.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16397..17221
                     /locus_tag="ACG8PN_001148"
     CDS             16397..17221
                     /locus_tag="ACG8PN_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861314.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17239..19371
                     /locus_tag="ACG8PN_001149"
     CDS             17239..19371
                     /locus_tag="ACG8PN_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4861315.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19388..19537
                     /locus_tag="ACG8PN_001150"
     CDS             19388..19537
                     /locus_tag="ACG8PN_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861316.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19534..19980
                     /locus_tag="ACG8PN_001151"
     CDS             19534..19980
                     /locus_tag="ACG8PN_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861317.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19982..20119
                     /locus_tag="ACG8PN_001152"
     CDS             19982..20119
                     /locus_tag="ACG8PN_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4861318.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20156..20449
                     /locus_tag="ACG8PN_001153"
     CDS             20156..20449
                     /locus_tag="ACG8PN_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861319.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20472..20669
                     /locus_tag="ACG8PN_001154"
     CDS             20472..20669
                     /locus_tag="ACG8PN_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4861320.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20680..21699
                     /locus_tag="ACG8PN_001155"
     CDS             20680..21699
                     /locus_tag="ACG8PN_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4861321.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21956..>22186
                     /locus_tag="ACG8PN_001156"
     CDS             21956..>22186
                     /locus_tag="ACG8PN_001156"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4861322.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFIS"
BASE COUNT         7722 a         3776 c         4917 g         5771 t
ORIGIN      
        1 ggattaagga ggaagagtaa tgcatatagg agataagata cataaattga gacggcttaa
       61 taacgaaaca atgaacgact tagttaaaca aaccggactg tcaaaatcat gggtatctga
      121 tattgagtct ggaaaaaagc aacgcgctga ctttaataag atatgtgcaa tagccaaaca
      181 ttataaagtt agcttagaat actttcagga tgattatttg gatagtgatg aacacgagga
      241 tattattcct ttgacagacg aaacaagagt taaatcagtg aaatcgcttg aacaaagcct
      301 aaagtatctg aataacaaac aagaacctaa ccttctagag atcaaactaa aagatactga
      361 ctcagtacca gaggtttatt acaaaggtca gaagttagag ggattgatag atgtaagcta
      421 tcactaccat acaacaactg atgtggtagc tggttcaaca aacgacatca atatggagta
      481 cctaggtgaa gtaactaagt atccagatat aaaaacaatc ggacacaaga gagatgtgta
      541 aatgaaagaa gctagaccta gagacgagat agacaaacta tacaagacca aacgatggcg
      601 agacctaagg caagtagtaa tagctaggga cttcggcatg tgccaagagt gcaagcgtcg
      661 agggcggaac acaaggggca cgatcatcca tcacatagtc gaggcgaggg aagacctgtc
      721 actgttctgg tccgtagata accttgaatg tatctgtgta gcttgtcaca acagagagca
      781 tccagagaga tcaggcggga agaagaaacc aaaacctaaa tcacatatcg ttaaaatgta
      841 ttcaactcct gaaagataag tttgcagcga aatgaaggta gcccccctac tctaaaagat
      901 taaagagtaa ggcttgagaa gaacggtgct gtccttcctt cgtaaaaaga ccgcttttca
      961 agttttttgg agaaaaagga aaaagccgac caatttaagc cggctttgga cgaagctatt
     1021 tcttagtcca tttgtttcct ttttgagaag taggaggtaa tcggtcgcct ggatcaattt
     1081 ttacttctcg tccgccttgg acatttccac cacgaggtcc cacttcttta taggttcctt
     1141 ttggtttatt gtcttcgccg ggtttataga gttctcccat aggaatccct ccctaaaaaa
     1201 atttcggcac agcactgccg ataacttaat tataaggatt gtgataacga tttcaatata
     1261 tcttttgaaa gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt
     1321 aaacggaaac ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga
     1381 caaattaaaa atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa
     1441 ggataccttt gagtttatcg ctgatgaatt gctgtctgtg gatttaatca gtaatccgga
     1501 cgtccataca atggctctct actccaattg gtattcgcaa tacgtttctt tagaaaaaca
     1561 gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga
     1621 ggcgagaggt gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca
     1681 gatggataca gcgtcgcgga atctccgttc ttttggcgct gatttaggac tatcaccagc
     1741 agccagagct aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt
     1801 ctaatatttt ggatatgtcc tacacagaac gtgtggacta ttggcaaagc tatcttgagg
     1861 agcaagcttc ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt
     1921 atgcggaacg attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta
     1981 aacggcatat gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg
     2041 aagaaaaagg tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg
     2101 actttggttc gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat
     2161 gggtacatcg tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta
     2221 aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg
     2281 ctttctttta gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt
     2341 tcttccgaca ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta
     2401 attctgtttt ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca
     2461 tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag
     2521 attgaaaaaa tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg
     2581 tgtcacaaat gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa
     2641 gaacgaaacc caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa
     2701 ttttgatcat ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa
     2761 gttttatatt gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg
     2821 cggaaatggc ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc
     2881 taaagaattt tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac
     2941 aaacggtgat gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca
     3001 ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc
     3061 aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct
     3121 ttgcatggct tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc
     3181 cccttatgtg ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga
     3241 aggaattatg ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta
     3301 ctaactaccg taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc
     3361 agataatttg ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga
     3421 gggtgacctc cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact
     3481 aatttcaggc ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta
     3541 cgtattggca aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt
     3601 caaacaatcg ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta
     3661 tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct
     3721 aaacactcac tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa
     3781 cgttatcaag aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc
     3841 tggatatgta ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga
     3901 acatttggaa gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc
     3961 ggaagaggct gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa
     4021 ttttgttggc atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga
     4081 ctggattacc aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat
     4141 gcctaccatt aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg
     4201 tgtcggtggt tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc
     4261 gcttgaaaca ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga
     4321 tcgagataac aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat
     4381 tcctggtgat tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat
     4441 ctatgacatt gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact
     4501 agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc
     4561 aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct
     4621 ttaccgatgg tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc
     4681 gtctaagcag tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc
     4741 cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat
     4801 ttccgattta atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg
     4861 ttcttttggc cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg
     4921 attgataccg tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag
     4981 ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg atagatgcgg agtttggcca
     5041 accggacgac tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt
     5101 tgattttttc cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat
     5161 tttccgaaat atcaatggag aagtagcaga tatggcgtta ataaaacctg gtgcttgcca
     5221 tccagtgatt gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg
     5281 cagttataag caagttattt atgtacatta catggaaatg ctccacttta aacaaccgag
     5341 gtttggtggc ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga
     5401 tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga ggtaatgaag ggctaaaagt
     5461 taagtttgct agcaatgtgg atgaagaagc taaaaaagct acagttaaaa acattgctga
     5521 tttttatcga caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt
     5581 acaacgagag ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat
     5641 cgcgatggtc tacaacgtgc cggaacattt cattgggagt aaccagtcga gttactcttc
     5701 acaggaacag ctcaatatgg agtttttgac atacaatcta gtaccgaccg ttaatcaata
     5761 tgaagcggaa ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata
     5821 taagtttaat gtcgcaagtt tgctaagagc tgacactcag gcaagggggc aattctatca
     5881 aatcatgcga cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca
     5941 accgataaat aaaaccggta tggatgatta tcatatttcc ggagacctat atccaatcga
     6001 tatggatcca acattaagaa aaacaaactc gtctaaaagc gtagccgaaa acggttaggc
     6061 ttttttagtt tgcaccgaag ggaggtgaaa ggatgaaaaa agtgacgtta agcggcgatg
     6121 tcgtggataa cgataccgcg tggttatatg actggtttgg gattgattgt atctcaccag
     6181 ggaaaatttc tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat
     6241 ctaacggtgg ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg
     6301 gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgtg
     6361 cttgcgaacc gcttagaatc tcgccaacag cacacatcat gattcacaac gcatgggtga
     6421 ccactagcgg caacgccgag gaattaaaag ccaatgcgga aatgttaagc agtgtggatg
     6481 agtctatcgt taatgcttac gagatcaaga caggactcga ccggaaaaag cttgctgatt
     6541 taatggcaaa agatacttgg ttaaacgcac aaacagcagt ggcggaaggt tttgcggatg
     6601 aaattatgtt tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa
     6661 aaaacgcggt aactaagttg aaaaatctaa tactcaaagc ggaaacaccg caagaagaaa
     6721 cgctcttgca gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca
     6781 attaaaaaat gcgtgggtcg aggcgggaag taaagtctct gatttaaacg cgcaactcaa
     6841 tgcagcattg gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt
     6901 aaaagcagca cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga
     6961 acaagtctta aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa
     7021 gtttatcaaa gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac
     7081 atctaatacg gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac
     7141 gactattcat actttggttc gtcagtttga ctctttgcaa gaatacgtaa acgttgaaaa
     7201 agtgaccact actagcggtt ctcgggttta tgaaaaatgg tctgatatta acccactgac
     7261 cgctttggat actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat
     7321 caaatacttg atcaaacgct acgcaggtat ttctacagta actaacagct tgctaaaaga
     7381 tactgccgaa aacattttgg catggttgtc taactggatc gcgaaaaaag tagttgttac
     7441 tcgcaataca aaaatcttgg cagctattga tggaatcaaa gcagcgcaaa agaaagatgt
     7501 tacagatgtt gatgggatta aagatatcat aaacgtccaa cttgatccag ctatcgaagc
     7561 tacatctatg tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc
     7621 tgatggatct tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg
     7681 taaaccaatc aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc
     7741 taaatatcca ctgtatattg gtgatctgaa agaagctgtt acattgtatg atcgcgaaaa
     7801 catgagcttg ctgacaacga atattggcgg gggggctttt gaaacagaca ccaccaaagt
     7861 acgggttatc gatcgatttg atgtgcagtt agtcgatgat gaagcagttg ttttagctac
     7921 atttacatct atcgcaaacg aagtgccagt aacaaacggt taaggagctg atttcttatg
     7981 attcttgatc ctaaaatgga tttagacgaa atcaaaaacg cactaaagat tgataccgat
     8041 gatgacgatg tggaagtaag acgtgcggtg caagctgcaa ttgcatacat tagaggggct
     8101 atcgggaatg ataagccctc tttttataag caagaaaatg acacggttga tctgattaat
     8161 ttagctattc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
     8221 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagatat
     8281 ttgctttttg tggaggagta gcgtatgccc cttattcaaa caggaaattt aaatcaacgc
     8341 atcaagtttg tccgagatac gacggtcaag gacgaggacg ggcaagttgt ccggacttct
     8401 acaaccattc ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg
     8461 tccattggta cggctttgga aggaacactg acgttcatta tccgctatca acaaaaatca
     8521 gagctaacca atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt
     8581 acgaaaggcg agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga
     8641 gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc
     8701 aacgagtgga aagcccagca cttagaaaag ctggggagta cgctcaagaa aagttacaac
     8761 aaaacacacc ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag
     8821 aacatgctaa ggaccatgtg gttacaagct cggtaaaaaa cggattgata gaagtcggct
     8881 atgataaaga tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc
     8941 caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg
     9001 ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat
     9061 tctgaatagc aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc
     9121 ggctcaaaca gtacctagcc ttccagttgg cagaattaca gagatattcg gcaactatga
     9181 agatttcgca agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt
     9241 atcgacctta aaagaggttg atgcctttta ttttgccctt gatgagatta tgagggggaa
     9301 tggttggcaa tgcgcataca cggaacaaac agatgatgag gatttagaag gtgcaaagcg
     9361 gattatcaaa cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc
     9421 aacagtagga tttgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa
     9481 agaattagta gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg
     9541 cgcaacttct aacacaacat acgcttcaaa cgtaccattc ttcattgcaa gtaaaggggt
     9601 ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga
     9661 aatcattggc gctaaaaccg tggatggtgt aaatgtaatt ggttcagaaa ctgaagcgcc
     9721 ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga aaacgcttat tcatgggatt
     9781 ggcaaaagga aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggagt
     9841 tgaattgcaa accgattcta tcgaagggga attcatttct gatgaacgtg gctatgtata
     9901 cttaacagcc gtagaatcag aagaaatgac cttacaaaaa ttcaaggact tggtaaataa
     9961 caaagcgggg gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa
    10021 gaaccagaac caaaaattga tacacaaggt tagccatttt ttggctagcc ttattttttt
    10081 gtaaaaacaa ggaggaaaac aaatgattga attgcaattg aaacttgacg gaaagaaaaa
    10141 aacattcaaa caacaagata tttccgcacg tgcaatgcgt gagtgtatca aattttacga
    10201 gaaagcggaa aaagcagacc taactgattt agaagcaatt gattcaatga ttgcaattac
    10261 agcagatatt ttccaagatc cagcagttac atttgatgct attttagacg gtttgactgc
    10321 gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa atcaatgaac tgggaaacaa
    10381 tgaaaaaaag cagacggcga gcaaaaagag ataagttttt ctgaagctag gaaagcaatg
    10441 gatcaaatct acaaagattt aatcgaatca ggttggacga tgagagatgt ggacgaagcc
    10501 gactatcatt atttgttaca cctttttgga gaagtggaga gtggcgaaga atatgtagat
    10561 ggtgctgatt tcatcaaaca atttttatcg gctgaagact tggtaaaact tgaggaagga
    10621 ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat caagctaggg attagtttag
    10681 atagcactaa ttttggtaac acgctggacg aaatcaatgc gaaagtcaaa caagctgagt
    10741 cgaatatgcg tgccaatcta aaggcttatg attcagcagg acgttcatac gaagcactta
    10801 gtcaaaagac gaaagacttg tctacggtta tggaagggca aaacgccaaa gtaagagaat
    10861 taacaaagcg ccgtgatgaa gcgattagca agtatggcga ggaatcgaaa caagttgcta
    10921 accttaacac acagataaac aatgctaccg caaaatataa tgcttacagt cgccagttga
    10981 atgacacaaa aaaagaattg gtgtattcca aaacagccgt caatgattta tctaacgaaa
    11041 tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg
    11101 aatctggtgc gtttgaagca aaacaaaaag ggctagccaa acaaacggaa ttatccgaga
    11161 aagctatcga agaacagcgc aaagttgtga aactgatggc tgatgagttt ggcgattcag
    11221 caaatgaaac cgaagatgca aaaagggcat tagaaaagtt agaacgacaa agccaaatat
    11281 ctagcaggca attagaagca ctcaaaagct ccagcgatca atcaggaaaa aaaatagaag
    11341 attttggcga caagtccaca aggtcagcta ggaaactgga tgggctaaaa gacaaattag
    11401 gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg attagcgcat aacgctatta
    11461 gcagtgtagt aagtggcgtg caaggcttgg ttggcgaagc agtaaacgca tcggattcat
    11521 tgatgaagtt ttccaaaacc atggagtttg ctaactttgg gaagtcacag atagaaagct
    11581 cgaaaaaaga aatgaaagac tacgccgata agacggttta tggtttagaa gaaattctga
    11641 acacaaccgc acaattggca tctaacggga ttcctaacta tacagaacta accaaggcgg
    11701 caggtaactt gaatgccgtt gcaggcggtt ctagtgatac attcaaatcc gttgccatga
    11761 tgctaacgca gacggcagga gctgggaaac taacaactga aaactggaat caattagcag
    11821 atgcgatacc gggcgcttca gggctgttac aagacgctat gttgaaaaac ggagcttata
    11881 caggaaactt ccgtgatgca atggcgcaag gtcaaatcac ttctgatgag tttaaccaag
    11941 cgattgtaca gttaggtatg aatgacggag cagttaaagc agctacttcc acagatacat
    12001 tgagtggttc ttgggaacag atgaaatcta ctgtaataaa tgggttacaa agtatcatag
    12061 acaaagtagg cgttgaaaat atcactggtt tcatcaacag agtaacaaaa gggattgaaa
    12121 attctattcc taaaattact caatttatag gttggttgag agatattgga acgtggatcg
    12181 ttgaaaatag agagccacta acatggattg tcggaatcat aggcggaatt acattagcag
    12241 taaaagcatt gaacgtagca agtatgttgc tggcaattac tggcggaaca ttggcagccc
    12301 cttttgtggc gattggtgta gcattaggcg cactagcagg tgctttggtg atagcttata
    12361 caaaatctga aacatttaga aatatagtca atgcggcttt tacagctgtg aaaaacgtag
    12421 ttatgagcgt tatcaataat ttggtggaat actacaaaat gttgtggagc gtgttgcagt
    12481 ggctttggga aaaaataaaa gaatgggctt catggattgg caataaattc attgaaatga
    12541 agaacagcgt tgtgaatacg gttcagaacc tatggaatgg tgtgaaaaac ttcttcagta
    12601 atggcgttgg agacacttgg aataaggtag ttggctgggt aaaaaacatt ttcaacaaag
    12661 caactgaatt gaagaacaaa gtttctgatg caattggtaa cctgtggaac ggtatcaaag
    12721 acacattccg tagaggcatt gatacggtat tcaattggtt ttcagaacta ccgacgaaga
    12781 tgaagaatgc cattattggc ggtaaaaatg ccattgttga tgcgttcaaa agtattttca
    12841 acgcagcact taaagcgata ggtaaaccag ttaacgcaat catccatgga gcttcatggg
    12901 tactagaaaa actgggtgct gacaaactcg aagaatggaa agtgccacaa tacgcaaaag
    12961 gaacaccaaa cggaggtcat ccgggcgggc ctatgatggt aaatgacggt agaggtgctg
    13021 aagcggtaat cacacctaac ggacaagcat ttatcccacg agggcgaaat gtagtgttga
    13081 atgcaccaaa aggcacacac gttctaacag ctgaagaaac agcttatatg actggaaaca
    13141 aagcaccaag atatagatac gccaaaggta caggcttttt cggaaatcta tggaacaacg
    13201 tcaaaggatt tgctggagat gttggaaaca agctgaaaga tgtagtcggc gatgtatggg
    13261 attttgtaac agacccggga gcgttggcta ggaaagtgtt aaatggtctt ggcgtactgg
    13321 aagggcttgt caaatatcct ttagatgttg gtaaaggtat tctaagcaag gctaccgaag
    13381 cattgacgaa caaaatcaca gaactattca gcagtggcag tttagacact tcaatgggca
    13441 tgcaaggcgt ttacaaatac ttggcggacg ttgcagttgc agtaatgaag aagtttccag
    13501 gctttgtggc aactagtggg tatagaccag gtgaccccta ttcgcatggt aaacgtaatg
    13561 ccattgatat tgcactacct ggtgtcacag gaggctcgcc acgctacaca gaagcagcca
    13621 attacgcatt tgagaagttt gcaaacaaaa tcggctatgt tatcacaaat ggcaaggttc
    13681 gtgaccgttc aggacaatca ggtacaggta ttcacaatga ttggagacca tggcccgatg
    13741 gagatcacta tgatcatgtg catttaaacg gtgtgaaaga cccacaaaac actcaaattt
    13801 caggagatag cgtgggaggc agtggggtag aaagatggcg caatgtagca attagagcgt
    13861 tgaaaatgac cggtcaatac agtactgcaa acttaaatgc attactaaat caaatgcgta
    13921 cagagtcaaa tggtaatcct aatgcagtta acaattggga tattaacgcc aaaaatggaa
    13981 caccatcaaa agggttgctc caagtgattg acccaacatt cagacagtat gcaatgccag
    14041 gattcaacag caatattttt gacccactat ctaacatctt agcttcaatc agatacgcac
    14101 tatcaagata tggctcacta acaaatgcct atcgtggagt tggttacgca aacggtggaa
    14161 ttgtaaacca acatcaaatt gcggaaatcg cagaaggaaa caagccagaa attattattc
    14221 ctttagataa ggctaaacga tcaagagcga tgcaattatt gtctatcgca atggacaaac
    14281 taggagtttc tcctaaaact tatggaaatg cgacaactgt ttccagtgat tacgaaatcc
    14341 tagaagccat tgaaaaacag gcggcaacaa cgaatcagct gttatcgtta ttgctggcat
    14401 ttttcaaagg aaatagccga actgatagag atttagcttt agatattcag aagatcttgg
    14461 ttaggaggat gtagatgcga actgtattac tgagaaatag aataaacgaa gaaattgatt
    14521 tgtctacgga ggattatttt gctactgggt tgagtaatat ggggtttgaa gtaaaaaaag
    14581 aacatgtggg acagtggggg aatttcagag aaagcagtga gaacgttgaa atatctgaat
    14641 ttcagtcatc tgtaatcatt tctgtgcatg ggtttcgaga aaaagaactg tacaattcac
    14701 ttgtgcagtt cctatcggaa ggtccgtttg aactggaatt tgcttttgac ggcgaaacaa
    14761 tggtaagaag atgcagtcta aaatctttaa gtaagactga aatcgatccg aaaacatcgt
    14821 tactaacaga cactttagaa ctatatttca catcaaactg gtattccgta aaacgcgaaa
    14881 aattaattca gcgaccgaat gtagtgaaaa cacgtggtaa ggtttttccg tataaaagat
    14941 cttatatcta cactcaaaac ttgtgggaga aaaaaggtgt attcaaattc aataataatt
    15001 ctgtatacct aacgaatagc aaggaacgga tgtccccgct aaaaattcgt gtgattggga
    15061 agtgttcaaa tccgtattgg gaagtaatcc aaaactcaca aatcatcgca acggatggat
    15121 acttcataga tatgactgaa acacagactc tagaagtatc aagccttttt gaagatacaa
    15181 cggcgatttt aaaagatatt gcaggggtag aatcttctgt ctaccaacaa caggattata
    15241 caaaaactaa ttttgttcag gccccgactg gagaattcag cattgtgttt catgttgggg
    15301 gggcagacgt agagattgag ttatacgagg agcgtgatct gttttgattt tagcagtcac
    15361 gctttttcat cgtgatttaa tgctttataa cgaacattta ttctctaaca gttttgaatt
    15421 tggtgcggac gagatcaacg aagaggctag tagctttacg atggataagt acgttcctgt
    15481 aaaaacaggc gattttctac tagcaaaata tattcctagt ggaaaatttg catattttgg
    15541 tgtaattacg tcgcaggaag acgaaaaaat cagttgtaaa agcttactta gtttagctga
    15601 tagtgagata ccgactgcac gtgtgtcagg agataactac gaagagcata tccggcggtt
    15661 gattgaatac tatttgctga atgatccaac gaaacaacta aaagatattt tagacgtcaa
    15721 agcagaaagt gcgacctctc attcgtatca agctaccgat acgaataaac acaagttaag
    15781 tgcgtatatt ctcaatggtt tcaaaaaata caacgtaaaa tggtatttca agggaattca
    15841 aaacagaaaa atctatacag gcatacgtgc tgtaaatgaa tcaatttaca ttaaagacaa
    15901 ttcttctgaa tttagcgatt gggatgtgtt tgttcaggcg ccgggcgctg gaaacgagaa
    15961 caagctatta atcgttgata aagcaatgaa agatatagag aaaccgataa tactgtcaac
    16021 atggtatttg gacgaagaaa acaatttgac acaagatgga tcgaaagaaa atatcacgaa
    16081 accaactgtc aatttggtta atatctacga tcaaaccgca gaagataaag catcctacga
    16141 agatgtggca aaatcagagt tgaaaggcaa tacgtattcg catgaaatca aagtgaacgt
    16201 tgtaagaaat gcaaaaaatt tgaatgtcga aacgattgaa acagggatgt ttgccacgat
    16261 ttcttataaa ggaaagatat acaagtcggt tttgacagct tggcgaatat caagtgataa
    16321 ggagtttgtg gaattgactt tcggaaacat aagaagtcgt tttatggatt attttgaaga
    16381 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    16441 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    16501 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    16561 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    16621 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    16681 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    16741 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    16801 actatgctca gttacttcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    16861 cgagctgact ctctctaaag gagaaatttt gtggagtggg actgcgttaa tgcatgatac
    16921 tcaaactgtc caaccttcaa aaaaaatgtg gcagacagtt tcaggttttt tattagtatg
    16981 gctcccatac gaaaacgggc aagcaattgg ggatagatat gtaactacgc ctttttataa
    17041 agagcgtgta acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    17101 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    17161 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    17221 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    17281 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    17341 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    17401 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    17461 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    17521 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    17581 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    17641 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    17701 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    17761 gaatcagata agcaagtaaa tgatcgtatt gataagacaa atgatcgtat tgatcaaacc
    17821 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag catcgacttc
    17881 ggggactatg attatagtgg gaatccgaac cttagcgcta aacttaatgc ttcaagtttt
    17941 tctttaggta ctggcgcaac agtcgcggat gataatgatg agattgtgtt taccttagat
    18001 ggcacaaatc aactatcgaa gtacgctagc aaagcgcaag tgccggtagc agaagggaaa
    18061 caatatacga ttagttgtga aattatgtta gaagatgggt tcactggcga cccttcagga
    18121 atacgtctgc aacacgcata tttgccaggc ggggttgttg ttttacagac agagacagta
    18181 cctaaaaatg aattgaatac gtggcagacg cttatcggaa cgcgaacggt aaaatacacc
    18241 tctgacgtac caaatgaatg gtaccctttg tttagagaca tccgaacact taaaccgacc
    18301 ggtaaagtta gactaagaaa aattaagatt gaagaaggct caacagccac cccatatcag
    18361 ccaaacttac ttgatgaccc ttactggcta ggtaaagcgc ctttgggtga gaatattgct
    18421 aataagtctg ttacgtttcc aatcaaatct agcgcctaca gcctatacca agctaacatg
    18481 gaagaagaat ttgttttagg tcaaacctat acaattacca tgaaggcaac caaaccacct
    18541 atccaaacgt tcattgttta caacgaggat agtggagatt atagatatgg taacttagag
    18601 ccagtagaag ggttagttga tacatggggt ctgactttta caccgcagaa ggttggagtt
    18661 aactacccca aacggttaac tattatccag tatccccaat caacaacagg tgcatgtcaa
    18721 attgactggc tcaagatcga aaaaggcaat actcgaaccc cgaatattag tgagtataaa
    18781 tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca
    18841 ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa
    18901 gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt
    18961 aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct
    19021 atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta
    19081 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct
    19141 atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac gatactaaca
    19201 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    19261 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    19321 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg
    19381 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    19441 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttctaaattg
    19501 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt
    19561 actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    19621 gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa
    19681 atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat
    19741 ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag
    19801 atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa
    19861 gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    19921 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    19981 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca
    20041 agttcgacta ttcgttccac tatgcattac agacgaagaa gcagataaaa tcattagcaa
    20101 agaagaaagc gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat
    20161 caatttaggg gaatggggaa caatcgcagg atcaattact gcgattgttt ctttgatttt
    20221 attagtaata agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt
    20281 aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat
    20341 gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag aaaaattggt
    20401 agaacacaca caacaaatta aaactttatt tagagaaagg tctcggtaaa aaagaaagga
    20461 gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa
    20521 cagtattgcc agctttatct gtattagtag ccacattagg caaagcatat ggatggaatg
    20581 gaacagatat ggcagttctg actatcaatg cagcagcaac atttttaggt gttatcactg
    20641 gtgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt
    20701 actgcgatga gcctattaac ggctcttttt ttattgccga ttaatgggtt tgcctatacg
    20761 attaacaatg aatttaattt aggtgtaaac gaaggtagtt ctcaagtagc aaataatcag
    20821 tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat
    20881 atgcaacgtt catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt
    20941 tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat
    21001 agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
    21061 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
    21121 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
    21181 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
    21241 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
    21301 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
    21361 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
    21421 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
    21481 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
    21541 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
    21601 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
    21661 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag
    21721 gggaagtaca taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa
    21781 tttaatagtt agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc
    21841 atgtaaccaa gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa
    21901 atacttacac aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc
    21961 taataaaaat tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa
    22021 cgtgggttat gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc
    22081 gaaaaatggt gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc
    22141 tgtaggaaaa atattagaag ttgctagtat taaacaattt ataagc
//
LOCUS       DAZUKA010000040        22169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000040 DAZUKA010000000
VERSION     DAZUKA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.040"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..2069
                     /gene="tdc"
                     /locus_tag="ACG8PN_001157"
     CDS             234..2069
                     /gene="tdc"
                     /locus_tag="ACG8PN_001157"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4861323.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2374..3852
                     /locus_tag="ACG8PN_001158"
     CDS             2374..3852
                     /locus_tag="ACG8PN_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4861324.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3818..5149
                     /locus_tag="ACG8PN_001159"
     CDS             3818..5149
                     /locus_tag="ACG8PN_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4861325.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5263..7815
                     /locus_tag="ACG8PN_001160"
     CDS             5263..7815
                     /locus_tag="ACG8PN_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4861326.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7822..9261)
                     /locus_tag="ACG8PN_001161"
     CDS             complement(7822..9261)
                     /locus_tag="ACG8PN_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861327.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9236..9418
                     /locus_tag="ACG8PN_001162"
     CDS             9236..9418
                     /locus_tag="ACG8PN_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861328.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9607..9969
                     /locus_tag="ACG8PN_001163"
     CDS             9607..9969
                     /locus_tag="ACG8PN_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4861329.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10156..11013)
                     /locus_tag="ACG8PN_001164"
     CDS             complement(10156..11013)
                     /locus_tag="ACG8PN_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4861330.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11133..12044
                     /locus_tag="ACG8PN_001165"
     CDS             11133..12044
                     /locus_tag="ACG8PN_001165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4861331.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12051..13702
                     /gene="mdcA"
                     /locus_tag="ACG8PN_001166"
                     /pseudo
     CDS             12051..13702
                     /gene="mdcA"
                     /locus_tag="ACG8PN_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13804..14103
                     /locus_tag="ACG8PN_001167"
     CDS             13804..14103
                     /locus_tag="ACG8PN_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4861332.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14093..15769
                     /gene="mdcD"
                     /locus_tag="ACG8PN_001168"
     CDS             14093..15769
                     /gene="mdcD"
                     /locus_tag="ACG8PN_001168"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4861333.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15773..16366
                     /locus_tag="ACG8PN_001169"
     CDS             15773..16366
                     /locus_tag="ACG8PN_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4861334.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16390..17466
                     /locus_tag="ACG8PN_001170"
     CDS             16390..17466
                     /locus_tag="ACG8PN_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4861335.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17491..18360
                     /gene="mdcB"
                     /locus_tag="ACG8PN_001171"
     CDS             17491..18360
                     /gene="mdcB"
                     /locus_tag="ACG8PN_001171"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4861336.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18471..19235
                     /locus_tag="ACG8PN_001172"
     CDS             18471..19235
                     /locus_tag="ACG8PN_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4861337.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19724..21817
                     /locus_tag="ACG8PN_001173"
     CDS             19724..21817
                     /locus_tag="ACG8PN_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4861338.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21842..22144
                     /locus_tag="ACG8PN_001174"
     CDS             21842..22144
                     /locus_tag="ACG8PN_001174"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4861339.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3788 c         4723 g         6479 t
ORIGIN      
        1 ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt gttcgctttt
       61 ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa aatctacgtg
      121 gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga ggtgttacag
      181 ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag
      241 atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa
      301 tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat attccttcag
      361 atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc
      421 atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc
      481 attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag
      541 cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa
      601 cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg
      661 gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc
      721 gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa
      781 tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct
      841 ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc
      901 aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag
      961 atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa
     1021 tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc
     1081 taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa
     1141 tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg
     1201 ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt
     1261 atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg
     1321 acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc
     1381 ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata
     1441 tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag
     1501 atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct
     1561 ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt
     1621 caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag
     1681 gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat
     1741 tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg
     1801 atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta
     1861 catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa
     1921 cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc
     1981 cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg
     2041 aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag
     2101 acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt
     2161 catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt
     2221 ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt
     2281 ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa
     2341 aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa
     2401 atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt
     2461 ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg
     2521 tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt
     2581 ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg
     2641 ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg
     2701 tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc
     2761 attttagtcg tttattggat catcactttt ttgaatttga aatttgatat ggcaaaaatc
     2821 ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta
     2881 gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca
     2941 tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc
     3001 atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca
     3061 gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt
     3121 ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc
     3181 gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg
     3241 tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca
     3301 gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg
     3361 aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca
     3421 atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta
     3481 aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga
     3541 ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag
     3601 agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc
     3661 ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc
     3721 attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa
     3781 gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg
     3841 gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt
     3901 atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt
     3961 gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa
     4021 tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt
     4081 ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc
     4141 aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag
     4201 cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga
     4261 atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg
     4321 attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat
     4381 cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt
     4441 cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa
     4501 gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac
     4561 gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt
     4621 agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat
     4681 tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata
     4741 tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt
     4801 acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt gtctacattt
     4861 ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa cgagttgttt
     4921 tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg
     4981 gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt caattgcttt
     5041 cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta tctggtttgt
     5101 attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg cccagataca
     5161 ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg aaaaaacttc
     5221 cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg gtacagattg
     5281 acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc gcaagaagaa
     5341 cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca gcagacaaaa
     5401 cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt gatcattgcc
     5461 tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt attcgtagtg
     5521 atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc attgaatggt
     5581 ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca agaaaaaatc
     5641 cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga tgtcgttcca
     5701 gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat tttgacggga
     5761 gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc gatcggcgac
     5821 cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt
     5881 gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca atcggtcgaa
     5941 caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc
     6001 attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg tatggattgg
     6061 catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga aggattgcca
     6121 gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc
     6181 atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg ttcagataag
     6241 acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc
     6301 tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga acagaaaaca
     6361 gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga aacagccaat
     6421 ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa atatatggca
     6481 acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc agaagtatta
     6541 tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac agctgcagaa
     6601 tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga ttcgaaacag
     6661 gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg attgatcgat
     6721 ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg gatctcagtg
     6781 aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca aattggctta
     6841 aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga tgaagagttg
     6901 attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca taaactgcga
     6961 attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat
     7021 gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa aggtagtgaa
     7081 gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac gattgcaaaa
     7141 gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa tttcttcttg
     7201 ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa tcgcccgcta
     7261 cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac tttatcttat
     7321 gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag agatccaaag
     7381 caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct actgatcatg
     7441 gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca aaccattctt
     7501 ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga gttgtccaag
     7561 ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt
     7621 gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat cggtacagta
     7681 agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct attcttagtt
     7741 gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga
     7801 gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag tcaaacgatt
     7861 gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta gagaatctag
     7921 agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa gatcggttag
     7981 ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct gctgcaagaa
     8041 cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat tcggtttgat
     8101 agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat ttggttcatt
     8161 gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat acaagtgtgc
     8221 atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg cttcaatcaa
     8281 tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca gagatgtttt
     8341 ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt cgaggacctc
     8401 taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt
     8461 ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa gataaatacc
     8521 ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg tgctgttggt
     8581 atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga taaagaacca
     8641 acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga ggtttcttaa
     8701 aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt tgttatgagt
     8761 gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc caagaataca
     8821 agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct gattgatttt
     8881 agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa aatcagggac
     8941 agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg attgtttgaa
     9001 aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac tgattcctgc
     9061 ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt gaaggtcaga
     9121 aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg ttatctttgt
     9181 atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt cggtaatgat
     9241 ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata cggagaaaat
     9301 aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg gagcatttac
     9361 tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca aaaaataggt
     9421 gggcaaacaa taataaacat tttaatagta gatggtagca agaccaacac aggcggattg
     9481 aacatttaag agttgataaa aaagaaaaat atcgaagtat ttgattagca actacttgta
     9541 ttcatttttc tattcttacg tggtatcttt gaataaagcg aatcaataag aaagagatgg
     9601 aagcagatgc taacgaaaaa agaactaatc gatcacatca atgaaacata caatttgtct
     9661 ccagaatata tttttaaaaa atttcctgat tattgcgtat tcagacatca aaaaagcaaa
     9721 aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa tctatgatga tgcaagtgac
     9781 cataggatca tcaatattct tgatttgaag gtcgatcctg aacttggcgg aattttgcga
     9841 ggaaaagagg ggtattttcc agcctatcac atgaataaag aacattggat ctctattgat
     9901 ctacgtaaaa tagaaaagct agatgaacta gcagatttga ttgatggaag ttttaaatca
     9961 acgcaatgaa tgtctaggac aatagagaag aggttgtgac agaaatgttt agcttcaaga
    10021 aataagaagg aatttacgaa aattgcttct caaatttttg ggaaatttcg gcttattttc
    10081 taagaaatta cttctggaac accgtttgtt cgaaaaaaga gctgcgacaa gaattttgtc
    10141 ccagctcttt tttttttatt ttcctaaata aaattcccgc atcgtttttg caatcatttt
    10201 gatcaaagga tgatcttctg tgaagctaga agataaataa attgttcgtg tcaagggcaa
    10261 atctttgatt tcccgcaaga cgaaaggatc atttttatcg taagaccaag aaacttttgg
    10321 gaaaaagcta acagctaatc cttgcttgac aatagaacgt aatgtcgcag gatcatctgt
    10381 cgtcgaactg atcgtcaatt cgatactttt cgcttcaagt gctgcatcca gtgtatgacg
    10441 gaaggcgttg ctctttgaca gcatcaagat gggggtgcgt tggatatctt ctaattgcag
    10501 atgttctttt ttagcaagag gatgtttctt aggaatggcg acataaacag gttctttcat
    10561 gataggaata ttggtcatgc caggcttcat ctccgaagtg atgtacagta caggatcggt
    10621 aatatcatct ggtaaatgat gttgcatgat cgttaattgg atatcaggat gcttttccat
    10681 aaaaattcgg accaagttcg gaatagaaac agaggctacc tctaaataca atatgacgga
    10741 agtaacgatc ttctcattga ccgaagacaa taatttgatt ccttcttcta atgtgtctaa
    10801 tgtcttgact gcatatggat aaaattcttc tgcataaacg gtcgtctcaa tgtttctgcc
    10861 gctatggtaa aacaaaggga cacctaattc gttttccaaa tgtttgatcg cacggctcaa
    10921 ggcaggttga gaaataaata attctttggc tgcttgggta acattttttg tttcgaatac
    10981 ttttttaaaa tactgcagat gaagcaattt cataattatc catcccatcc ataccttttt
    11041 gttatggttt tttctttttt tggcattttt tattatagca gaaattgact aagatagcag
    11101 tggaaaaagt aagtgctgag gaggcgaggc tcatgagtac aggtttctta tttcctggac
    11161 aaggcagtca gaagttaggg atgttagcgg atatccctga aatgtacatg gaacgtttag
    11221 aaaatgtgac ggggtatcgg ttagttgata aagaagaaaa ctatcaagat acggttttta
    11281 tccagctagc attactgaca aaagcagtat tttatttaga tgaaatggaa aaaaacggta
    11341 ttcagcctga tattgcagca ggacattcga ttggtgcttt ttgtgcagct gttgcttgtc
    11401 gatctttgag atttgagaat gcagcggaac tagtctatca ccgcgcagtc ttaatgaaag
    11461 aagcttatcc aagcggttat gcaatgggcg tagtagtagg tgtgacgaga agcgaagctg
    11521 aggaaattgt agcaaaaaca tttgatcctg accatccagt ttatctgtca aatgaaaatt
    11581 gtccgatgca acatacgtta tctgggagta tcgaaggttt atccgttacg ttggcagaag
    11641 ccaaacggca ccaagcgcaa acagcgaagc tgctgaaagt ccctgtgcca tctcactgtc
    11701 tcttgatgaa tgatacggtc aagaaattca agccgtatat gcagtctgtc catctatctg
    11761 atgctagatg tttgtatata aaaaatacgg atggaagagc cactcaggac gcagaagaga
    11821 ttcgtaaaga tttgttggaa aacctagctt tccctgtgca gtggaaccaa atgatggact
    11881 tggcaaaaga attaggaatg gatctgacga ttgaatttcc ccccggcaat actttgacaa
    11941 agctgattca tacaaaattt ggtgtagata aagggatccg tacgatcaat ctagaccaac
    12001 atggtattga tgacgccacg tttctatatc aaaaatggag atgactaatt atggaaaaac
    12061 caaaccgcaa ctgggcttct aaacgcgaaa aaaaagcgca gcgattggaa aaaatcagca
    12121 acttgctcaa tggcaagtat gtagaaactg aaaaaatcgt agaagtaatg gaaaaactga
    12181 ttgctcccgg cgacaaagtt gtattagaag gcgacaatca aaaacaagca agttttcttt
    12241 cagaaagtct tgaaaaagta gatgcagaaa aagtgaatga tctgcatttg atcatgtcta
    12301 gtatctcacg accagaacat ttgaatattt ttgaaaaagg aatcgcaagc aagatcgatt
    12361 tctcttatgc tggtgcacaa agcttgcgtg tcgcccaaat gattgaagac ggcacaatga
    12421 aattaggtga tatccatact tatttggaac tttatggacg cttattcatc gatttgattc
    12481 caaatgttgt tttagtagct gcagataaag cagataaaga cggcaacttg tacacaggtt
    12541 tcaatacgga agaaacacca acaatcatcg aagctgcagc tttcaaagat ggtatcgtga
    12601 tcgttcaagt taacgaagtt gtcgattctt taccacgtgt agatatccct ggtgactggg
    12661 tagacgttgt cgtgaaagca gataaacctt atcaattaga agctttattc acacgagatc
    12721 ctcaaaacat cacagaattg caaattctaa tggcaatgat ggcaatcaaa ggaatctacg
    12781 cagaacacgg cgtacaatct ttgaatcacg gtattggatt caatacagca gctatcgaat
    12841 tgttactgcc aacttatggt gaatcactag ggttaaaagg gaaaatcgcg aagaactggg
    12901 tattgaaccc acatccaacg atgattccag ccatcgaatc aggttgggtt gaaagcatcc
    12961 atagttttgg tggcgaagtt ggtatggaaa aatatattgc tgctcgccca gatgtctttt
    13021 ttgtcggtaa agacggcacg atgcgcagta accgaacatt atcacaagct gcaggacaat
    13081 atgccatcga tatgtttatc ggttcaacct tgcaattaga ttacgaagga aactcttcta
    13141 ccgtaacaaa aggacgttta tctggttttg gcggtgcgcc taacatggga cataatcctg
    13201 gcggtcgtcg tcattctaca ccagcatggc aatcacttcg cgacaatgat gatccattag
    13261 ggaaaggcca aaaattagtt gttcaaatgg ttgaaacata tggttcaaat aaaaaaccag
    13321 tctttgtcga agaattagat gcactagctg ttcaaaaaca agcaggctta gccaatgcaa
    13381 tgcaccagtg atgatttata gcgaagatac tacacatatc gtgacagaag aaggaattgc
    13441 ttatttgtac aaagcaaaaa caaaagaaga acgacaagca gcaattgcag caattggcgg
    13501 tgttacacct cttggaatga ctagcacgaa agaatcacta gatagcttgc gtgcagccgg
    13561 catcgtgaaa ttaccagaag atctaggcat caaacgtagt gaagcgaaac gttcattatt
    13621 agctgcacaa accatcgatg atttgatcga atggtcagat ggcctgtatg aaccaccaat
    13681 gaaattcaga acttggtcat aaaattggcg agaaaaacct tttgtcgcca tttacccaga
    13741 tggaagaaga aagacgaata agcagaacgt gtatgctctg ttttacaaaa aggggagaga
    13801 tccatggaac aattagctta taaatatcca acgaaacagc cgatcaataa gaaaatccat
    13861 gtaggagtgg tcggttcagg agatttggaa atcttgatgt ttccaacaga aaaaacagaa
    13921 tctactgtaa aaatttgtac aggaagcgat ggcttcggcg aagtatggaa caatgtatta
    13981 acgagatttt ttgatcgtta tccaatttca gcagatatcg tgatcaatga cttcggtgct
    14041 acaccaggag ttgtgtacct acgattgacg caagcaatgg aggaactttc agatgaaaaa
    14101 tagttttgta gaattaaacg ccagagaacg agtgaacgct ctacttgaca atggacgtga
    14161 attgattgat ccattcgatc aaatcatcgc gccaagttta ccagcacaag gaatcgtccc
    14221 tgaaagtgat gacgggatcg ttgtcagccg tggcacgatc aatggcaaac aagcagtagt
    14281 aatcgctatg gaaggaaaat tccaaggtgg cggtatcggt gaagtttccg gtgcaaaaat
    14341 cgttgctgca cttgaacatg tgttagaaga aaataaaaaa ggaaatgaga tctttccagt
    14401 cattgtacta gatacaggtg gtgttcgttt gcaagaagca aactacggac tgttatctat
    14461 ttcagaaatc ggaaatgtaa tcattgctct aaaacaatat gtaccagtca tcggtttgat
    14521 tccaggacgt gtaggatcat ttggcggtat gtcgattact tcagcattaa tgagttattt
    14581 aatcgcaaca aaaaaagcga gagtcggttt gaatggacca gaagttattg aacaagaagc
    14641 aggcgttcga gaatttgatt ctagtgacaa agacttgatt tggaatacat taggatctcg
    14701 tcaacgggta gaatcaaaaa tcattgatga attagtgact gatagtgtag aagcaatcaa
    14761 agctgctgtc atcgcagcaa tggacgaacg aaaagacacg catcgcagtg aaagctcagc
    14821 attttattta tcattacttg attctttaga tctttcaaaa ccaatggcca ttgacgatta
    14881 caatgaagca attgctaacc atcaagcaaa atcaattgac ttaccagaag tagcagaagg
    14941 aaatgaacca gctgcagcaa gtgttggtta cgattggttt gcggcactaa ctggcatgaa
    15001 aaatccaact tcttctatta gtactgtgta ttatggtaaa tcaagtaaat tcatagaaga
    15061 agccatcgtg acatcaatcg tacctgatcc taaaaacccg atgtaccgtg tacgaaacgg
    15121 tgaagttggt ttagttgaag gtttccgcat gggacaaatt ttggatcacg tttacgaaga
    15181 agataaagac aaaaaagtga aacggccaat catcgttgtg atcgatgtac caagccaagc
    15241 atacggctat aacgaagaat tgatcggtat ccatgtggct ttggctaata gtgctgctgc
    15301 ttatgcaaaa ctccgtcaag ctggacatcc agttattgga ttgattgcag gaaatgctat
    15361 ttcaggtgca tttttagcac atggattaca atcaagccgc ttgattgctt taaacaataa
    15421 agaaattacc gtccaagcga tgtctaaaga aagtgctgca cgtgtgacaa aacgttcggt
    15481 tgctgaaatc gaagcagctg ctgaaaaagt tccagctatc gcttacgata tcaataatta
    15541 tacaaaacta ggagcgatct atcatttcct agaagatatc accgaccaag ctgcttcaga
    15601 acaaaatgtc agcaaagtca tcgaagcaat caatgaagcc attcaagatg tccgcaatac
    15661 aaatgacacg atgctgacaa atcgatacac aaacgaaaca gccaaaacat tcggacgtgt
    15721 cgaaacaaat aaagtccgtg ccaaaatgga cgaagaatgg gatgcataaa aaatgattca
    15781 agcacatgat attgtccttt ttgataaaca tgaaatagaa gggttcacct tgcctgaatg
    15841 ggtcgttcta actgaaaaga tgttcggaac agttcgtcgt gcgtcgacgg atcacgcaga
    15901 aatactaccg attggtttac gtggacctgc ccgcaatcag agatggtcaa cagaaatctc
    15961 aaaacatgcc gtcaaacaag tcattcatcc gtgggacatc atcgaacaag aatcgtatag
    16021 acaagagaaa atcatggatt atcctgttta tcagcaattt catgctgcta gaaacatatt
    16081 atcaggatgt aaatggggaa tcggcggcag cttaggattt gaattatcga ctgggattcc
    16141 ggcagtaaaa gaaaccagtg attttgattt gctgctgtac gcaaattcac cgattgaatt
    16201 gccaattcag gtaattcaat cgcatcctgc tttttttgag caattcgata cccaagtagt
    16261 gactcggcaa ggcggttttt cactaaaaga gtatttgaga gagccagaga aaaaaattct
    16321 attgaaaaca gtcgaaggac ctaaattaac caaagaactt tggtaataaa gaaaaagaga
    16381 gagaaagaga tgtttgaaaa caaaaaaatc aaaatcaacg taagtgactt ccagaaagta
    16441 tcagctgtaa cggctgttat gatgcaaacg atattgagct ttgctttggc aaacagccac
    16501 agcgcaacaa atacagcttt tcttggtacc ttttacgtca tcgttaaata cacagcacca
    16561 atgtttattt ttgcaattgt ttataatatg gtaaaaacaa gtcagcattt gtcttattgg
    16621 gaatttttga aagaaaagtt ttttgaacta gtcgttccgt atcttctatg gacgacggct
    16681 tatctttggc tatttccagc agtccaacaa caaactccgt atacggatac tgcaagcttt
    16741 ttactgaaat atgtcacagg tgatggagcg cctcatttat ggtataccgt tatgatgctg
    16801 cagatccaat tgttcatgcc gttttttgtt tggctcggtt acaaagtatt tgctaataaa
    16861 aaatatgtat ggccagtatt gattggttca acgattttat atgtcgcatg gtatctgttc
    16921 tatcaaacac aagtattgac tgggccttat catgacagct ggtacttatt agatcggttt
    16981 gttgctagtt tcatgattta tggggtagcc gcatttgttt atcacgaaaa agtctatcaa
    17041 tatttggaca gagtacgtta tcttttctta cctgttgcct tagttatcgc gttcttttct
    17101 gtccgttcat tgctcgcaca tccaggagac ttgagctttg cgaatgcacc ttacttgaat
    17161 acgatccaaa gtttatacag tttagtcatc atctttgctg tcttcatagg cgcttccaaa
    17221 atgattgtaa acgattcgcc aaaattgcca ttgttcaaat ggttgtctgt ttatgcttat
    17281 cgtacgtatt tagccaatgt atttgttttc caagtactgt tattattgtt taaagatagt
    17341 tggctgcagt taccttcagg agtcatgatt cttgtagctt atctaatgac ggcaagttgt
    17401 gcatttgctt taagctggtt acttcatatc atttgggtag ctatcaaaaa aggtttcagc
    17461 aagtaatagt aggaagaagg agtgagtgtc atgataaatg acgaacgaaa aaaagcagtg
    17521 actcagcaac tcgctgcgtt ggtagaggaa gcattgatcg cagaagtaac attaagtcca
    17581 aaacctggat tagtggatgc actggatgcg ggtgcacatt ccgatatgga ctatgcttta
    17641 tttcttaagt ctgctaagac gctaactcct ttttttgaag aaatggctca aatagcatgg
    17701 catcatgcta ttgatcagga gttaagagaa agaattgctg agattgggcg aaaagcggaa
    17761 aaggcaatgc tgattgctac aaatggcatc aatacccata aaggagcgat ttgggcaatg
    17821 gggctattga tcagtgtcat tgcacaaaaa atcagtatgg aacagccttt ttctttatcg
    17881 aaaatacttg atgatgtcag caagcttgct gcttttcctg atacaaaata tcgtcataaa
    17941 aaaaccactc atggtcatag ggtgaagcaa aaatatggcg taaatggtgc atatgaagaa
    18001 gcgatattag ggtatccgca tatcaggctt gcaattcaag cagctaagga gctagataat
    18061 gattcatatg tacagaaaca aatgcatatg ttgttagttt tgatgagttc gctagatgac
    18121 acatgcgtat tgcatcgcag tgatcagaaa acgttagtgg aaatgcaaaa acttgctcat
    18181 gctgctagtc aaagtgcttt accaaatcgt ccatttacag cactgttgca cttttgtcag
    18241 gaaaaccaaa tctctcctgg aggaagcgca gatctcttgt ctgcaagctt gttcatgctg
    18301 aagatagagg aattatggac tgccgacaca tacatttgtc cgatgttact caatgactaa
    18361 ataagacaaa attgctaaaa aaacagttta tgcatctggt tattgactct tccttttagc
    18421 aaggctatca taaaaatagt tttttgtaat aaaagataac gaggaagaca atgaaaaaaa
    18481 tcaattattt agcaatcatt ttgttagtgg gcctatgttt gtttgctctg ccgacccaag
    18541 gattcgctgt cgcgggtggt catggtggag gtacatctag cggtcgaagt tcgggcagag
    18601 gttcatttgg tggtcgttca tatgggggca gttattttgg cagtcgttct tacggcagag
    18661 attcttatgg gtattatggt ccaggtacag gtccttctag ttatgagcaa acgggatttc
    18721 ttgcattagc tgttttgtca gtaggagggg tttctgcttt ttctatcgtc aagaataaaa
    18781 gacaaaagaa aaggctgaaa gcggagatat tgtcccgcat gtcaggaacg gctaaagaaa
    18841 agaaagcaca gtttctggaa atccaaaaga tattcatgaa gatccaatcc gcttgggaag
    18901 aagaaactcc tgaaaaagcc agaagctgtt atacagacaa gctgtttgcc gaacatcaga
    18961 aagttctgca aaaaaacaaa gcagacggtg taagaaatca tactaaaaaa ataactattc
    19021 aggaagtagg aaattatcga cagatccacg aaaatagttt tgccatccga attgatttca
    19081 gatgctgtga ttattcagtc gatcgaaaag atgggcgatt gatctcagga agtaagacac
    19141 aacgacagta tttttctcag atatggtatt ttgatttttc tgagaaagaa aaaagatggc
    19201 aagccgattt tattcagccg attatgctgg attgacagga aatatttatg aacggagttg
    19261 tgacaaaagc cttgtcgcag ctcctttttc tgaataaacg gtgtgccaga gatagcctat
    19321 cggcaataag gcacgatttt gtaaaatatg ggaagcgatt tttcaaaatc gcttcttatc
    19381 gctcaaggct gaacatctct gtcacaacct ctccactaaa tggtgtttaa aagcttgcat
    19441 ctgcgataat aagaacaacc aaacgaaaaa taggaagcac tattttagtt tagttgttct
    19501 tattacttga tgcaggacag ctttttcaca acctcttttt tgtatagatg ggcaaaattt
    19561 ttgtaataat aggcgtatgt ctttttcgtt gaggagatga aaaagcatat ggcttatttt
    19621 ttgtttgctt tgcttacttt ctttccactt caagtggcag aaatatctac tttttattat
    19681 gtaagcggaa ccatatactt gaaatcagaa ggagatcatt tctatgaaaa caagaacaat
    19741 tgaaatactt cacagaatca tgacagccca atatccagta aaagcaaaac atctagcaag
    19801 tgagttcaat gtcagtcagc ggacgatccg tcaggaagtt cttgaagcga atatctggtt
    19861 gagcagtaaa aagcttccgg agatccgtac aattcgcaac aaaggttttt tgttgaagct
    19921 ggaaaaggaa aagcaagctc gattagaaac tgaaataaaa gatgtgaaag acggcttttt
    19981 gaatcggcgc gaacgtattt ttgatctagt tttagccgtt gcttacagcc gaatcccgat
    20041 ccatctaaac aaaaaagaag aagaatttca aatctctaaa agtacattag atgaagatat
    20101 gagacggtta cgagcaacac tggtcagtta tgggatagag atcgtcagtt atggaaagca
    20161 aggactggtt tataaaggtt cagaacgatc tatccgtacg atgatctatg attttatcaa
    20221 tcagaatctg ggaatgaccg acttctttat gaatcaagat gcacaattaa caccggctca
    20281 aaaaatcttt ctccattatt ttcctttggt ggaaatcaaa aaaatacagg agatctattc
    20341 caataagttg ttgaaacaag aagatgatat ttataaaaaa caaatcctgc tttttacatt
    20401 gatctggatc cagcgaatca ggaaacatga atttatctct gccattaatt ggaaaaatac
    20461 agaaataaaa gacagtagct tttctgcatt tattgaccac atgatcgagg aatttcaatt
    20521 ggacaatgtc ccgcaagtag aaagaaatta tcttcgtttc actattgaga cgttcaatgc
    20581 tagagatatc tccaattcaa tcgagtgggt acaagctcaa ttactaacga tccaattgat
    20641 ccaatttgta gagaacgaga cacacgtacc tttccatcta aaggaagaag ctttattcga
    20701 aaatctctat aagcacatgg ctgcactgat tgtaagaatc aagaatcata tccaagtcat
    20761 gaatccattg aaagaaaaca tccgaacaaa ttatttccca atctaccgcg cagtcactca
    20821 ttttgttcct acgatcgaag aagtaattgg tggcaagata ctggaggacg agatagcttt
    20881 tcttgtcatc cacttttcga cgatcgccag tgtaatcaag cgagacttga attatgttta
    20941 taaatcagtc gttgtctgta accatgggat ggctactgga aatctgttag cagagaatct
    21001 gaaagaaaag tttcctcaga tcgagattac tgcagtttta agttcaaaag aaatcgaatt
    21061 agtagataaa ttggatgtca atctggtttt cagcaccttt caattgaatt atcgaaataa
    21121 gccgctttta gttgtcgatc cgattttaac tgagacgaat atcttggtta tagaagactt
    21181 tttgaaaaga aatgaagaac tacagcggtt agagcctaac agtcatgaat cgacggagtt
    21241 attcactcaa ataatagatg tcatgaaaga aagtggtggc cgagtagatc gaatcatcta
    21301 tggcaagtta gaaaaattat tcgaaacaaa caatttagaa atcaacaaaa gggagatcca
    21361 gcctatgtta aaagatattt tgacggacag caatatcatg atcaaagaaa aagccactac
    21421 ttgggaggaa tcgattgaac tagctgctag accattggtt aaagaaaaca ttgtcgaaga
    21481 gcgctatgtc gaagcgatga ttcgtgcagt gaatgattac ggaccgtata tcgtgattgg
    21541 caaacacatg gcactagccc atgcccgacc agaagatggg gtcaataaat taggagtcag
    21601 cgtagctacg attgagcaac caatcgattt cggtaatcct gaaatggatc cagtaaagat
    21661 tattttttgt ttggcagcag tagactccta ctcgcatctg aacatcatga aagaactaat
    21721 tgaattgatc aacaacgaag agaagttgga tcgattgatt gaatgcacaa atatcgaaga
    21781 tttcaaacaa ctgctttttt cagtagttga gaaataataa atgatacggg ggagaacaaa
    21841 gatgaacaaa ttgaacattt tattcgtatg cggtgcaggt ttaggaagta gttttgcagc
    21901 acagatgtct gctgaggatg ttttgaacaa attaggagta gaagccaaac tggaccattg
    21961 tgatatttct tccgcagttt ccatgaaacc agatgtgatc atcacagcag aaaattttcg
    22021 ttcgcagttc gaaaagttcg cagttgatcc taaaacggcg atgatctatt tgaaaaatat
    22081 tgtttcaaaa aatgaaattg aagaaaaaat caccccagtt ctgcaagata agggagttat
    22141 ctaaatacaa aaagaaaaga gggtaaaaa
//
LOCUS       DAZUKA010000041        21636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000041 DAZUKA010000000
VERSION     DAZUKA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.041"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..573
                     /locus_tag="ACG8PN_001175"
     CDS             361..573
                     /locus_tag="ACG8PN_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFS4861340.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            576..788
                     /locus_tag="ACG8PN_001176"
     CDS             576..788
                     /locus_tag="ACG8PN_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861341.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            928..1083
                     /locus_tag="ACG8PN_001177"
     CDS             928..1083
                     /locus_tag="ACG8PN_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861342.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1080..1304)
                     /locus_tag="ACG8PN_001178"
     CDS             complement(1080..1304)
                     /locus_tag="ACG8PN_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4861343.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1382..1570
                     /locus_tag="ACG8PN_001179"
     CDS             1382..1570
                     /locus_tag="ACG8PN_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861344.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            1709..1837
                     /locus_tag="ACG8PN_001180"
     CDS             1709..1837
                     /locus_tag="ACG8PN_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861345.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            1896..2231
                     /locus_tag="ACG8PN_001181"
     CDS             1896..2231
                     /locus_tag="ACG8PN_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861346.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2464..3405
                     /locus_tag="ACG8PN_001182"
     CDS             2464..3405
                     /locus_tag="ACG8PN_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFS4861347.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3407..4297
                     /locus_tag="ACG8PN_001183"
     CDS             3407..4297
                     /locus_tag="ACG8PN_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS4861348.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4340..5140
                     /locus_tag="ACG8PN_001184"
     CDS             4340..5140
                     /locus_tag="ACG8PN_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4861349.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5155..6006
                     /locus_tag="ACG8PN_001185"
     CDS             5155..6006
                     /locus_tag="ACG8PN_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4861350.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6072..6206
                     /locus_tag="ACG8PN_001186"
     CDS             6072..6206
                     /locus_tag="ACG8PN_001186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861351.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            6207..6668
                     /locus_tag="ACG8PN_001187"
     CDS             6207..6668
                     /locus_tag="ACG8PN_001187"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4861352.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            6694..7146
                     /locus_tag="ACG8PN_001188"
     CDS             6694..7146
                     /locus_tag="ACG8PN_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861353.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7252..7446
                     /locus_tag="ACG8PN_001189"
     CDS             7252..7446
                     /locus_tag="ACG8PN_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861354.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7477..7863
                     /locus_tag="ACG8PN_001190"
     CDS             7477..7863
                     /locus_tag="ACG8PN_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861355.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            7860..8063
                     /locus_tag="ACG8PN_001191"
     CDS             7860..8063
                     /locus_tag="ACG8PN_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS4861356.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8060..8356
                     /locus_tag="ACG8PN_001192"
     CDS             8060..8356
                     /locus_tag="ACG8PN_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861357.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            8432..8848
                     /locus_tag="ACG8PN_001193"
     CDS             8432..8848
                     /locus_tag="ACG8PN_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4861358.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9109..9246
                     /locus_tag="ACG8PN_001194"
     CDS             9109..9246
                     /locus_tag="ACG8PN_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861359.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            9656..9886
                     /locus_tag="ACG8PN_001195"
     CDS             9656..9886
                     /locus_tag="ACG8PN_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861360.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10045..10878
                     /gene="terS"
                     /locus_tag="ACG8PN_001196"
     CDS             10045..10878
                     /gene="terS"
                     /locus_tag="ACG8PN_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS4861361.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            10871..12286
                     /locus_tag="ACG8PN_001197"
     CDS             10871..12286
                     /locus_tag="ACG8PN_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFS4861362.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12298..13827
                     /locus_tag="ACG8PN_001198"
     CDS             12298..13827
                     /locus_tag="ACG8PN_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4861363.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            13745..14797
                     /locus_tag="ACG8PN_001199"
     CDS             13745..14797
                     /locus_tag="ACG8PN_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS4861364.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            14799..15116
                     /locus_tag="ACG8PN_001200"
     CDS             14799..15116
                     /locus_tag="ACG8PN_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861365.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15243..15866
                     /locus_tag="ACG8PN_001201"
     CDS             15243..15866
                     /locus_tag="ACG8PN_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS4861366.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            15881..16771
                     /locus_tag="ACG8PN_001202"
     CDS             15881..16771
                     /locus_tag="ACG8PN_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFS4861367.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            16794..17009
                     /locus_tag="ACG8PN_001203"
     CDS             16794..17009
                     /locus_tag="ACG8PN_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861368.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17021..17362
                     /locus_tag="ACG8PN_001204"
     CDS             17021..17362
                     /locus_tag="ACG8PN_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861369.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17362..17733
                     /locus_tag="ACG8PN_001205"
     CDS             17362..17733
                     /locus_tag="ACG8PN_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861370.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17726..18121
                     /locus_tag="ACG8PN_001206"
     CDS             17726..18121
                     /locus_tag="ACG8PN_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFS4861371.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18123..18500
                     /locus_tag="ACG8PN_001207"
     CDS             18123..18500
                     /locus_tag="ACG8PN_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFS4861372.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18501..19109
                     /locus_tag="ACG8PN_001208"
     CDS             18501..19109
                     /locus_tag="ACG8PN_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4861373.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19109..19450
                     /locus_tag="ACG8PN_001209"
     CDS             19109..19450
                     /locus_tag="ACG8PN_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861374.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19692..>21636
                     /locus_tag="ACG8PN_001210"
     CDS             19692..>21636
                     /locus_tag="ACG8PN_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4861375.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWL"
BASE COUNT         7876 a         3141 c         4538 g         6081 t
ORIGIN      
        1 ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg ttcttttaat
       61 aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata ccgacaaaaa
      121 aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat actagttttg
      181 taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt ctccccagac
      241 atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag catatttgca
      301 gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg ggtatgaagc
      361 atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag tcaaagagag
      421 ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat cgaaaaaaga
      481 aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt agaagtagat
      541 tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg agcaaagcgg
      601 agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg aaggaattta
      661 aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata acaattgatg
      721 tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga gttagagctg
      781 tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt gctgctctcc
      841 tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct tggaggtggt
      901 gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg gattttgaat
      961 gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt agcagaagtt
     1021 acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc taaatatgat
     1081 taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat ttttccatct
     1141 ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc gatttttact
     1201 gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc tttgacattc
     1261 caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt cacctccatt
     1321 tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa ggagaagtga
     1381 tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac aacgcaggaa
     1441 acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag ataggcaagc
     1501 tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa atagggagat
     1561 tagattatga agaagacaga cacacttttt ataggattca ttttggggtt attagtgatt
     1621 gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt tttaatcaat
     1681 ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa aaatcggtaa
     1741 agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca agcaacatat
     1801 cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag caaagagtca
     1861 catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt gaaaatctta
     1921 tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt acagcccaaa
     1981 aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg
     2041 ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa
     2101 ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata
     2161 cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg
     2221 aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt
     2281 gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct
     2341 ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact
     2401 atttaactga tcctgattat gctaaaccac catatgaagc accaattgat gaggaggatg
     2461 aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga ccgtaagaaa
     2521 ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa atctccttat
     2581 caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag tgagcccgct
     2641 tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa
     2701 aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat
     2761 attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc taatcaattt
     2821 ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca agttcaacat
     2881 tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg tggtcaaaaa
     2941 tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta
     3001 gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga tggtagtcaa
     3061 gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca aacgacgtta
     3121 ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac taagaaagaa
     3181 attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg taaacgtgaa
     3241 gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg tacgagacgt
     3301 atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat
     3361 aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg caacaaatga
     3421 atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag gtcaattagg
     3481 acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg tgcttcatga
     3541 caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc
     3601 agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg
     3661 gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac
     3721 aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc
     3781 ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa
     3841 aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga
     3901 agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg
     3961 gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa
     4021 gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa
     4081 tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga
     4141 gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga
     4201 tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg
     4261 attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt
     4321 ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa
     4381 ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt tgatgaaagc
     4441 gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga
     4501 attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca
     4561 tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt
     4621 gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca
     4681 aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa
     4741 caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg
     4801 caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa
     4861 acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagataacat
     4921 tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg
     4981 gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa
     5041 atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa ctaaaacgct
     5101 tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa
     5161 actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt
     5221 cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt
     5281 tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa
     5341 acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt
     5401 tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat
     5461 caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga
     5521 aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg
     5581 gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg
     5641 ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg
     5701 attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc
     5761 ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat
     5821 tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa
     5881 gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga
     5941 tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa aggagcgatc
     6001 gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg caaattatag
     6061 gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg aagataagta
     6121 agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac gtctacgaga
     6181 atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt tgcggtagta
     6241 gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt cgtcagcagt
     6301 atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc gcagactttc
     6361 gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg ccacacttaa
     6421 gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac gaactttggc
     6481 cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct tcaggaacat
     6541 taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag gctattggag
     6601 aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt atgaaaaaat
     6661 tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa taaatgtttt
     6721 ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa aaatattgaa
     6781 gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac gagatacaca
     6841 ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc gaaaatcgac
     6901 aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga tgattccagt
     6961 ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga aaaactagga
     7021 agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga tggggctgag
     7081 tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca aacaggtagt
     7141 atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga tgaatacttt
     7201 gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt catggacgca
     7261 tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt cgaagtagtg
     7321 ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat tagcattcag
     7381 caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt agtgacttgc
     7441 gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca ccaactcata
     7501 tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg gtttccaatt
     7561 gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga agatttaaga
     7621 caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga gtggttgaaa
     7681 agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt ttctgataaa
     7741 aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt ggcttgtaaa
     7801 caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga ggaagcggaa
     7861 tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat gtactaaaga
     7921 caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg gcaagtcagt
     7981 ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta aatgatgatc
     8041 gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta gtcatggtat
     8101 cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga aatcaaaatt
     8161 caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt ttcgtggata
     8221 tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga tggaatgcga
     8281 taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg atatggaact
     8341 aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc caacacattt
     8401 attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg tagatttcaa
     8461 acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg agcggatggc
     8521 aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga aggcaccgaa
     8581 gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag cggagagaga
     8641 cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac tctactacag
     8701 cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac gaggatacgg
     8761 agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag aagcatacaa
     8821 aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga tagtgtaggg
     8881 tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
     8941 caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct tcgatagaca
     9001 gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt gttcttatta
     9061 tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat ggataaagaa
     9121 atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa caaggctagt
     9181 gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa aatccaagtt
     9241 tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta tcagaaaaat
     9301 ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt taataatttc
     9361 aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac aaaataaaaa
     9421 atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa gacgcaaagg
     9481 tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga aaggactagt
     9541 acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta ggttgctatt
     9601 acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct acataatgag
     9661 aaactactgg tatatatcgc taactaatga atatcctcga accattgatg attgttcagt
     9721 gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa tgaccagaga
     9781 agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg gtttctatga
     9841 tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg caagaaatgg
     9901 cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt aaagatattg
     9961 attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact caacctaaga
    10021 agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg tgatgaagcc
    10081 aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc atctgaatta
    10141 aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag cgctgaattg
    10201 aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc tcctattggt
    10261 aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa gagaaataag
    10321 aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac cgataatgag
    10381 aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga agaaatacga
    10441 cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga agctggacta
    10501 aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc gattgaaaaa
    10561 aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat tagcagaaaa
    10621 aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg gattagcaac
    10681 cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata cagaactgat
    10741 ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg cggaaataat
    10801 tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa caaacgggcg
    10861 gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg ccattgatta
    10921 ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc ctgatgaatg
    10981 gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc gttctggtca
    11041 aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa cgtgtcgacc
    11101 ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg tactttgggc
    11161 agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt ggacaaagac
    11221 caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa cagcgactaa
    11281 accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag atgaagcttc
    11341 tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt ttgataataa
    11401 gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt cccacaattc
    11461 agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac gtacaaacaa
    11521 agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg ctcgtgtccg
    11581 tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg aaacggttga
    11641 attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg ttgctcatat
    11701 tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta gaattgctac
    11761 cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa caggatatgt
    11821 catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag tgatgattaa
    11881 agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac ttatagaaga
    11941 caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag aagacgattt
    12001 ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag aagaaaatat
    12061 gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata gttcgttaat
    12121 caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac gtttagaaag
    12181 taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg cactggcttt
    12241 agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg tgagtttttg
    12301 attagtacag tattatcttt agagagatat aagaagctac gtgttaaata tgcaacacaa
    12361 attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc attttttgct
    12421 gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga caatagacca
    12481 aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat gtattcaatt
    12541 gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga ttacgatgta
    12601 tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa aaatgatata
    12661 gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga ttccatgaga
    12721 ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt agctttccga
    12781 ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa tattgaacct
    12841 tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta tgactcttac
    12901 cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct gacatatgct
    12961 tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga tgagaccgat
    13021 aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg ttattgtgat
    13081 tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga tcaacaaaac
    13141 gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat gagcaaaggc
    13201 actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc tactgcaaaa
    13261 gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat ggatctattg
    13321 aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt gtttaaatca
    13381 aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa aacaaatact
    13441 aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc tgcttttctt
    13501 aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt ctctagatct
    13561 ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc atacgtatcc
    13621 aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga aaatgaaatg
    13681 atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg cggaggtggc
    13741 aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg atcaagcaag
    13801 gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg aagcactgaa
    13861 agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt atccagaaat
    13921 gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg aaaaaaatta
    13981 taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat tttttccacc
    14041 atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat ttaaaattct
    14101 tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct tagaggaaat
    14161 tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa acgttgatat
    14221 accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat ggtcaggaaa
    14281 gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac tctctaatgc
    14341 tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca ccatgagatt
    14401 agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca aagcgataga
    14461 aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa aatcatattt
    14521 agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag tttgccggat
    14581 ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca taggtgaaaa
    14641 tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac caaaattagg
    14701 aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg agtttgcaca
    14761 tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt tttgaagcag
    14821 tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct attgagtctg
    14881 agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac gtggttattg
    14941 gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc gttaatttaa
    15001 cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat tggtatgaaa
    15061 agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt aattaggctt
    15121 ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt gaatggtggg
    15181 gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg ggcgaaagga
    15241 gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt gccgaaggtg
    15301 acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat gaagaagctc
    15361 aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa gcttggaaac
    15421 aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa tctaagaaaa
    15481 acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa gcagaaaaag
    15541 cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct ggattagaac
    15601 tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt gttagttcgg
    15661 atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat gcaattaatg
    15721 agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt ggaactcaac
    15781 aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa gaacaattag
    15841 gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa catctttaaa
    15901 taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac caaatacacc
    15961 ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga cagaaattaa
    16021 atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg gcggagaata
    16081 tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt ttagaaaatc
    16141 aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa atgaattaac
    16201 taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa aattgattac
    16261 tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga ttttgaactt
    16321 gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa gaaaagatat
    16381 cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac tatgcttgat
    16441 ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa caaaaattgt
    16501 tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt ctaattacgg
    16561 tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg caatcgatac
    16621 taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg caaaaacaac
    16681 ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg cttcaaactc
    16741 tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct tttttgatag
    16801 gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg aaatatagag
    16861 taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga tttatcacag
    16921 cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt gcaattttgg
    16981 aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat cgctaaaaac
    17041 ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac gcataactaa
    17101 tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa atgatatttc
    17161 atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa aaaatgaatc
    17221 agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg cttcaacaac
    17281 aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag ttattggttg
    17341 tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac ttatgaagat
    17401 agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat tactattcaa
    17461 gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg tcaaatcgat
    17521 ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac atataaatta
    17581 tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac tcaaaaggcc
    17641 agtggcttaa ttttttctct atttgctact aagccttttt actatcctag tcattgtgaa
    17701 gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa atgcagaaaa
    17761 gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa tatccttcgt
    17821 taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg taaaatcagg
    17881 taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg gtcgaattgt
    17941 tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc ataggactaa
    18001 aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc ttgcaaatat
    18061 gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga aaggaaagta
    18121 acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc tatcctgatg
    18181 ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc gttttatcag
    18241 taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct ttcttaattg
    18301 atattgcatt gattgataat aaacctaata gaaatttaat taatgaatta gtctcacgtt
    18361 gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca aaagcctatt
    18421 tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt ccacaatata
    18481 ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt atttctgtcg
    18541 aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta tatggtactg
    18601 cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa acaacacaag
    18661 attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca gaagttgaac
    18721 tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg aaaattgtta
    18781 aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt gcatatagac
    18841 gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag tttgctctag
    18901 attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat ccaacttgga
    18961 agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat gaagatgatg
    19021 aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt ccaattgaag
    19081 aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga ttaaattaac
    19141 gaaaaaagat gaagaaggta aatatgagca agtacaattt aaatctgctg aatttctacc
    19201 aggcccagtc gtagaagaag cagcagaagt aatggaggga atgcaaaatg ctgtagatgg
    19261 aaaatcagta tcagaagcat taagtcgagc ttattcattt attgcagata ttttgtttga
    19321 aggacagttt actggtgaag attatcgtca agggatcgat gctcgagaaa ttgcttcgtt
    19381 gacaggtaag ttattaaaat ctgttactgc aggctttgat gaaacttata cagaaacgaa
    19441 aaaaaagtaa gcgaagctct caaatatcct ttttttaaac attcgattac ttatcgagaa
    19501 ttagatttaa agacgcaatt actcgaagca ggttggacgt taccagaaat tgaacgtact
    19561 gactttgacg agttaatgcg tctttttgct tttcgagatg cagtaaaaga gcatgaggat
    19621 gttgagtact acgataactt cacccaattt taggaggtga tactttgaat aacgatgacc
    19681 taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat aatgctgtca
    19741 aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt ggcagttcat
    19801 taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta actagaatcg
    19861 taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat cgagtagata
    19921 cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca gaaattatga
    19981 agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct actgctttga
    20041 atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta actcgttcaa
    20101 ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca actgatcaag
    20161 ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta gacgcacaaa
    20221 cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt gctaaaaaga
    20281 tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt tctgttgaag
    20341 aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt aaatcattaa
    20401 gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat gcaaaaacag
    20461 ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta gtagattcag
    20521 atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa tcgttcttaa
    20581 aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta tttaacgaaa
    20641 ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct gcagctaccg
    20701 cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa agcttcaata
    20761 attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg gcaatagctg
    20821 ctgctaattc atttgtttta attattggtg cagtcgttgg tgcaatagct gttttcggtt
    20881 actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg aatgatgtga
    20941 aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat acttggaatt
    21001 ctgctaaaga aatggcatca aatgcagttg agggtgtcaa agatgcttgg tcaggaacaa
    21061 aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt ttatttgata
    21121 aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg tctaatatga
    21181 agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa aaatggaatg
    21241 gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata aagaatgcat
    21301 ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag attggtaaaa
    21361 acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt acctccatga
    21421 tttctggtgg atgggaagaa acaaaaaata atatgattgc tgtttggaat aatattaaag
    21481 aaagtgccat aaatatttgg gaatctataa agaatatatt tgtaagttat tttacaaata
    21541 tttattttgc tgcactcaat atttggacag ggttcaagct tacattgata aatatttgga
    21601 atgaagtggt aaatcaagct aaatcgattt ggttaa
//
LOCUS       DAZUKA010000042        21574 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000042 DAZUKA010000000
VERSION     DAZUKA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.042"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACG8PN_001211"
     CDS             complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACG8PN_001211"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4861376.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACG8PN_001212"
     CDS             complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACG8PN_001212"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4861377.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACG8PN_001213"
     CDS             complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACG8PN_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4861378.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACG8PN_001214"
     CDS             complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACG8PN_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4861379.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACG8PN_001215"
     CDS             complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACG8PN_001215"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4861380.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACG8PN_001216"
     CDS             complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACG8PN_001216"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4861381.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8318..8788)
                     /locus_tag="ACG8PN_001217"
     CDS             complement(8318..8788)
                     /locus_tag="ACG8PN_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4861382.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8834..9259)
                     /locus_tag="ACG8PN_001218"
     CDS             complement(8834..9259)
                     /locus_tag="ACG8PN_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4861383.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9326..9595)
                     /locus_tag="ACG8PN_001219"
     CDS             complement(9326..9595)
                     /locus_tag="ACG8PN_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4861384.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9606..11201)
                     /locus_tag="ACG8PN_001220"
     CDS             complement(9606..11201)
                     /locus_tag="ACG8PN_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4861385.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACG8PN_001221"
     CDS             complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACG8PN_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4861386.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACG8PN_001222"
     CDS             complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACG8PN_001222"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4861387.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15667..16641
                     /locus_tag="ACG8PN_001223"
     CDS             15667..16641
                     /locus_tag="ACG8PN_001223"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4861388.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16811..18211
                     /locus_tag="ACG8PN_001224"
     CDS             16811..18211
                     /locus_tag="ACG8PN_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4861389.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18289..19011)
                     /locus_tag="ACG8PN_001225"
     CDS             complement(18289..19011)
                     /locus_tag="ACG8PN_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861390.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19090..19596)
                     /locus_tag="ACG8PN_001226"
     CDS             complement(19090..19596)
                     /locus_tag="ACG8PN_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4861391.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19719..21518
                     /locus_tag="ACG8PN_001227"
     CDS             19719..21518
                     /locus_tag="ACG8PN_001227"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4861392.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5997 a         4711 c         3872 g         6994 t
ORIGIN      
        1 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
       61 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      121 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      181 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      241 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      301 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      361 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      421 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      481 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      541 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      601 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      661 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      721 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
      781 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
      841 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
      901 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
      961 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1021 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1081 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1141 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1201 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1261 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1321 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1381 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1441 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1501 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1561 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1621 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1681 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1741 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     1801 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     1861 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     1921 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     1981 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2041 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2101 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2161 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2221 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2281 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2341 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2401 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2461 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2521 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2581 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2641 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2701 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     2761 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     2821 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     2881 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     2941 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3001 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3061 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3121 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3181 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3241 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3301 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3361 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3421 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3481 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3541 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3601 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3661 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3721 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     3781 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     3841 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     3901 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     3961 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4021 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4081 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4141 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4201 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4261 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4321 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4381 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4441 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4501 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4561 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4621 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4681 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4741 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     4801 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     4861 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     4921 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     4981 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5041 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5101 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5161 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5221 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5281 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5341 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5401 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5461 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5521 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5581 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5641 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5701 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     5761 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     5821 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     5881 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     5941 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtaaatttt gagtactcga
     6001 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6061 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6121 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6181 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6241 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6301 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6361 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6421 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6481 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6541 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6601 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6661 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6721 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     6781 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     6841 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     6901 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     6961 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7021 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7081 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7141 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7201 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7261 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7321 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7381 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7441 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7501 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7561 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7621 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7681 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7741 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     7801 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     7861 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     7921 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     7981 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8041 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8101 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8161 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8221 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8281 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8341 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8401 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8461 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8521 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8581 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8641 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8701 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     8761 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     8821 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     8881 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     8941 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9001 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9061 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9121 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9181 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9241 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9301 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9361 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9421 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9481 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9541 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9601 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9661 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9721 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
     9781 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
     9841 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
     9901 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
     9961 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10021 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10081 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10141 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10201 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10261 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10321 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10381 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10441 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10501 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10561 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10621 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10681 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10741 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    10801 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    10861 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    10921 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    10981 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11041 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11101 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11161 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11221 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11281 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11341 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11401 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11461 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11521 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11581 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11641 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11701 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    11761 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    11821 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    11881 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    11941 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12001 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12061 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12121 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12181 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12241 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12301 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12361 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12421 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12481 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12541 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12601 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12661 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12721 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    12781 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    12841 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    12901 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    12961 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13021 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13081 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13141 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13201 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13261 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13321 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13381 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13441 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13501 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13561 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13621 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13681 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13741 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    13801 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    13861 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    13921 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    13981 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14041 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14101 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14161 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14221 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14281 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14341 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14401 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14461 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14521 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14581 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14641 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14701 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    14761 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    14821 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    14881 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    14941 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15001 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15061 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15121 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15181 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15241 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15301 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15361 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15421 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15481 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15541 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15601 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15661 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15721 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    15781 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    15841 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    15901 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    15961 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16021 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16081 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16141 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16201 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16261 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16321 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16381 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16441 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16501 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16561 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16621 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16681 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16741 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    16801 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    16861 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    16921 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    16981 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17041 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17101 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17161 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17221 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17281 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17341 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17401 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17461 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17521 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17581 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17641 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17701 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    17761 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    17821 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    17881 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    17941 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18001 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18061 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18121 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18181 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18241 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18301 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18361 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18421 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18481 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18541 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18601 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18661 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18721 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    18781 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    18841 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    18901 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    18961 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19021 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19081 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19141 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19201 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19261 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19321 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19381 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19441 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19501 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19561 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19621 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19681 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19741 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    19801 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    19861 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    19921 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    19981 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20041 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20101 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20161 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20221 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20281 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20341 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20401 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20461 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20521 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20581 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20641 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20701 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    20761 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    20821 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    20881 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    20941 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga attgatcgta gccgatgcta
    21001 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21061 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21121 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21181 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21241 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21301 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21361 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21421 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21481 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21541 aaagagttag tccagaatgg gctaactctt tttg
//
LOCUS       DAZUKA010000043        21471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000043 DAZUKA010000000
VERSION     DAZUKA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.043"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..1270)
                     /locus_tag="ACG8PN_001228"
     CDS             complement(245..1270)
                     /locus_tag="ACG8PN_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4861393.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1545..3230
                     /locus_tag="ACG8PN_001229"
     CDS             1545..3230
                     /locus_tag="ACG8PN_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4861394.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3244..4527
                     /locus_tag="ACG8PN_001230"
     CDS             3244..4527
                     /locus_tag="ACG8PN_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4861395.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4597..5466
                     /locus_tag="ACG8PN_001231"
     CDS             4597..5466
                     /locus_tag="ACG8PN_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861396.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5463..6299
                     /locus_tag="ACG8PN_001232"
     CDS             5463..6299
                     /locus_tag="ACG8PN_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861397.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6325..7392
                     /locus_tag="ACG8PN_001233"
     CDS             6325..7392
                     /locus_tag="ACG8PN_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4861398.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7389..8216
                     /locus_tag="ACG8PN_001234"
     CDS             7389..8216
                     /locus_tag="ACG8PN_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4861399.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8261..9286
                     /locus_tag="ACG8PN_001235"
     CDS             8261..9286
                     /locus_tag="ACG8PN_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4861400.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9283..11466
                     /locus_tag="ACG8PN_001236"
     CDS             9283..11466
                     /locus_tag="ACG8PN_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4861401.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11466..12596
                     /locus_tag="ACG8PN_001237"
     CDS             11466..12596
                     /locus_tag="ACG8PN_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4861402.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12593..13270
                     /gene="pgmB"
                     /locus_tag="ACG8PN_001238"
     CDS             12593..13270
                     /gene="pgmB"
                     /locus_tag="ACG8PN_001238"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4861403.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13354..13881
                     /gene="msrA"
                     /locus_tag="ACG8PN_001239"
     CDS             13354..13881
                     /gene="msrA"
                     /locus_tag="ACG8PN_001239"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4861404.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14082..14768)
                     /locus_tag="ACG8PN_001240"
     CDS             complement(14082..14768)
                     /locus_tag="ACG8PN_001240"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4861405.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14848..16386
                     /locus_tag="ACG8PN_001241"
     CDS             14848..16386
                     /locus_tag="ACG8PN_001241"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4861406.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16506..16973
                     /locus_tag="ACG8PN_001242"
     CDS             16506..16973
                     /locus_tag="ACG8PN_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4861407.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17507..17935
                     /gene="rplM"
                     /locus_tag="ACG8PN_001243"
     CDS             17507..17935
                     /gene="rplM"
                     /locus_tag="ACG8PN_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4861408.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17951..18343
                     /gene="rpsI"
                     /locus_tag="ACG8PN_001244"
     CDS             17951..18343
                     /gene="rpsI"
                     /locus_tag="ACG8PN_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4861409.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18431..19657)
                     /locus_tag="ACG8PN_001245"
     CDS             complement(18431..19657)
                     /locus_tag="ACG8PN_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861410.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(19729..20031)
                     /locus_tag="ACG8PN_001246"
     CDS             complement(19729..20031)
                     /locus_tag="ACG8PN_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861411.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            20579..21298
                     /locus_tag="ACG8PN_001247"
     CDS             20579..21298
                     /locus_tag="ACG8PN_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4861412.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21300..>21471
                     /locus_tag="ACG8PN_001248"
     CDS             21300..>21471
                     /locus_tag="ACG8PN_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4861413.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVA"
BASE COUNT         6953 a         3804 c         4460 g         6254 t
ORIGIN      
        1 gtaaatgtgt ttaaaaaaac gaactataaa aacttataca caacgtggtt aaaaatagtt
       61 ttttcttccc tgtggaaaaa atagtttaac acaaacatta tagttatcca cagctatcta
      121 aaacagatgg aaaataagaa aaattcgcta aaataccggg agggggtaac gagggatgag
      181 tgattgaaaa aagaacagag taactgactt atcctcagtg tttctgttct tttataattc
      241 tgttctacat ttcttgttgt tggatcgaat ctgtgagagt aatctcggtt gaaagaatga
      301 ttcttttttc ttcagggtat tggtggtgga ttagttggaa taaaagttct gtggctttga
      361 ttcctaactg atttgtcgga atgtctatcc gagtcaactg tggcgaaaat agggaggaca
      421 ataagggatc actgaatcct gcgatcgaaa gatcatcagg tatctgcttt ccagattgtt
      481 ctgccacttt gtaaatactc aatgatttta atgcatcggt agaaaaaatc gcagttggtc
      541 tttctcgtag cttcattaaa tcattcattg ctgccatgct atcttctaag cgaaagccgc
      601 cgtctataat ccattcatta cggatatcta ggtggtgatc aaataagcat tgacggtaac
      661 cattgagacg atcattggat acataaactt ctgtcggagc atggagacag ccgatatgac
      721 ggtgtccatt cttgatcagt gtttccacca aatcgtaact atcctggaaa ttatccgtgt
      781 ccactgagta gatgttcgtg tagtcgtttc cgacttttcc gattaccaca ataggaatat
      841 tgtattgatc caattcaaaa agaagtgttt catccatcgt agagcttaaa ataataattc
      901 ctcggatcat tttttcttgg atctttttcg cgcatttttc tagctcttct tctggtgttt
      961 ttgccgtttg tagaatcaag tcgaagttat tttgttcaga aacggcagaa atggattgaa
     1021 taacatctga aaaaaaagga ttgccatagg tcgtatgaga agtacgatga gataatgcta
     1081 gaatcgcgtc aaaaccattt cctgacagtc ctcttgcaat tttgctcggt tcataattca
     1141 attctttgat tgcttgaagt acacttgctt ttgctttatc agagatattt ggctggttat
     1201 ttaatactct ggagacagtt gattttgaga cattagcttt tttcgcaatg tcataaatag
     1261 taggtttcaa ttttatatgc acctcctttt tgggagcgtt ttagaataat tccatagtac
     1321 aatagacatt cttttttgtc tatgattttg aaaatttagg aatagcttgc atgatcatga
     1381 gagaccatat ttttttaaga agctattaat tattgataca taagctttct tatttgtatt
     1441 tttgtgtata tcaaaattat ttaaaaaaat gctattgaca gtaagcgttt tccataatat
     1501 gatcaaatcg ttgggaacgc tcccaaaaaa gaaaggggaa tccgatgtat acaaaacttt
     1561 tacaagcgat ttatcgcgac tcagatttgt ctgagaaaac cgcctctatg attgaacaaa
     1621 aagtcagtgt ttatcagcag aagaaaggcc aggcaggcaa actgcattgg gacgaagaag
     1681 atattttctt gattacttat ggggatcaat tttttgaaga gcagcaacca actttgcgta
     1741 catttaagaa attttatcaa aagtatttgg cagatacatt tagagttgta catttcttgc
     1801 catttttccc atatacatct gatgatggtt tctccgttgt aaattatcag gcaatcaacg
     1861 accgtttggg cacatgggaa gatatagaag aaatgagaag cgaaacacgg ttaatgttcg
     1921 actttgtctg caatcatatg tctgccgaaa gtacttggtt tcaggaatat ttaaacttga
     1981 atgatacgta caaagatttt tttattgatt tgccactgga tacagataca agcagcgtga
     2041 ctagaccaag gaccagcccg ttacttacaa cgttcattga caaaaacggg aaagaaaaac
     2101 acgtatggac gacttttagc gaagatcaag tagacttgaa ttttaaaaat ccagaagttt
     2161 tattgaaaat gatcgatatt cttttgtttt acttagaaaa aggcgcttca tttattcgct
     2221 tggacgctgt gggattttta tggaaagaac taggaacttc ttgtatacac ctgccagaaa
     2281 cgcataaaat catccaatta ttccgtgcaa tcgctgatga agcagctcca ggaacggtat
     2341 tgatcactga gacaaatgtg ccacacaaag ataatatcag ttattttggg aatggcgaga
     2401 atgaagcaca gatggtctat cagttttcgc taccaccttt gacccttcaa gcgattcgtt
     2461 cgggtaatac aagtaagttg aaagaatggt taaaaaccat cgatttcgca tcgaaaaaaa
     2521 caactttttt taatttttta gcctcccatg acggtatcgg attgaatccg gtacgaggta
     2581 ttctgccgga aaaaacgatc cttgaattag tggaacagct gaagaaagag ggtacccgag
     2641 taaactacaa gaaaaaccca gatggaacga ccagtccata tgagataaac gctacgtatc
     2701 tagatgcgtt aagttctcca acagatacga cggaatggag aagcaaacgc tttttagctg
     2761 ctcattcatt tttggtaacg ttaccaggcg tgccggcaat ttatattcaa agtgtgctgg
     2821 gaagtcgaaa tgattacaaa ggcgtagaaa aaacaggaga gaatcgaaaa atcaatcggc
     2881 aaaaattttt gttagcagat attgagaaag agttgcagac agatccatta agaacagcag
     2941 tatatggtga attgacccag atgctatcta ttcgaaaaaa agaaaaagcc ttccatccga
     3001 atagtttgat ttacttactg gactcagcag attcttttat cagttttatt cgcgaagcga
     3061 aggaagagaa agtcttagtt atccataatt tttccaatca atcagcaggt tatcttttac
     3121 caaaaggaga ttacctagac ctgttttctg gacaaattat ccattgcagt caagaacgaa
     3181 agttgatatt ccagccgtat gaatatatgt ggctgaaaat aataaaataa gaggtggaaa
     3241 aaaatgaaaa aaattattgt tactacctgt ctgctagcta ctggatttct tcttactgct
     3301 tgtggtgaaa gtcaaagctc taacgaatca caacagacaa cgattcaatt catgcactct
     3361 tctgtcgaac aggaacgatt aagtgtgatc aatgaactgg ttgctgattt tgaaaaagaa
     3421 aatccagaca tcaaaatcaa gcaagtgccg gtagaagaat cagcgttcaa tacaaaaatc
     3481 gttacgttag ctcgttccgg taaattacca gaagtaatgg aagtcagtca ggattttgct
     3541 aaagtgatgg ataaagatga actgattgat cagacggcag tccaaaacgt tatcgaggaa
     3601 gctggagaag ataattatta tgacggagcc aaaaaccttg ttcgttcaga agatggaaaa
     3661 agctatattg ctgcaccaat tagcggctgg gttcaaggta tttggtatga caaagaaaaa
     3721 ttatcagaag ctggtttttc agaaccagaa aattgggatg atattttaaa aattgctcaa
     3781 catttcacag ataaagagaa taaacaatat ggaattgcta tgcctactgc tgaaggaaca
     3841 atgagtgagc aagcgttctc tcaatttgct ttgtcaaatg gagcaaatgt tttggatgat
     3901 gaaggcgagg taacgatcga tacggaaaag atgagagaag cattgtcgtt ttatcaaaac
     3961 ttatcccaat acacgatgcc aggctctaat gatgtgacag aaatcaaaga tgctttcatg
     4021 aatggaacag caccaatggc aatctattca acttacattt tgccttctgt ttatgaagcg
     4081 gggaactcag aaaatatcgg ttttgctatc ccaacacaaa aagatcaagc tgtctatggc
     4141 acagtatctg gattgacgat ttcttctggt ctagacgaga aacaaaaaga ggctgctgaa
     4201 gcattcacag ctttcttatc tcaaccgaaa aacatggaaa agtgggtctt gatgtctcct
     4261 ggtggtgctc aaccagtgaa taaacaagta gtcgaactgg atggttacaa agaaaatgaa
     4321 gtcatcaagt cttttggcga gctgccatct gagattgcta gtgcttttga tgaagtacaa
     4381 gtctttggac tagtcgggga aaagaatttc acgaaaatgg gagatatcac aagctcaggt
     4441 gtgatcggaa aagcagtgaa ccaagtaact gtcggaaacg aagatgtcga tcaagcttta
     4501 gctgacgcac aaaaaaatac gaaataacaa gaagttctgc caatggtgtt cgaaaaaagt
     4561 agatagctat tggcagatta tcagggggag gtttcaatga atcgtccaag gaaacattct
     4621 gacttaaaat tagccatggg acttttagca ccaagcatgc ttttattagc tgttttagtg
     4681 ctgattccaa tgctttccaa tattcagatc agttttttcg atatgccgct gaatcctaac
     4741 tcagacgccg tttttatcgg attaagcaat tatcttgaaa ttttaagtga tagtgaattt
     4801 tatcgttctt taggtttaac agttgcttac acattcttag ttgtagcagg cagtacagct
     4861 ttaggattag ccgtagctat tttgcttaac cgcaagttca gatttaggaa aacggtgcgt
     4921 tcactgattt tattaagtta tgttacgcca tctatttctt tgatttttgc ttggaaatat
     4981 atgttcaata atagttatgg aatcgtcaat gatctgttag tagataggtt gcagcttttc
     5041 aacgaagcac cgctgtggtt cgaccagcca gttgctagct tctttatggt cgttttgttt
     5101 gctatctgga gatactatcc gtatgcgttt atttcatttt tggctatttt gcaaacagtc
     5161 gacaaaacgc tttatgaagc agctgaaatg gacggtgcaa atgcttggca gaagttcaag
     5221 atcgtgacac tgccggcaat caaaccagta ctagcaactg tgattgtttt acgatctatc
     5281 tggatgttct atatgtttac agatgtctat ctactgacga ataaagtcaa tatcttaggc
     5341 gtttatttgt atcagacagc ctttgcgttc aatgatcttg gaaaagcagc agctatatcg
     5401 attctattgt tcatcattat tgcagcggtc gtcttgctct cacgaaagaa ggtggacttg
     5461 aaatgaaaaa taaaaagagc aggaaaatct tattttacgc gacgattttg ttgctattag
     5521 ctgtcaattt gtttccattt ttgatcatgc tgatgacatc attcaaggat tcacaagaag
     5581 caattgcaac gacacaaact atttttccga aagaatggac ttttgaacat tatcaggata
     5641 ttttcaatgc tgagattttt ccattcatca tgtatttcaa aaatagttta gtgatctcct
     5701 tgatagcctc cgtaactgca gtagtgattg ggatcagcgg ggcatacgct ttgtcaaaac
     5761 tacgcttcaa agctcgctcg aaaatcaatg ctagctttta caccgtttat atgttttccg
     5821 gtattttgat cgttgttcca ttatttaaaa tcatttccgg tctaggtttg tatgatacca
     5881 atacagcact gattatcacg atggtcgttc aaacacttcc tactgcgata ttcatgctaa
     5941 aaagttattt tgacacgatt ccgactgata ttgaagaagc agcaatgatc gatggtctta
     6001 gccggatcca gatcattatt cggatcatta taccattggc tatttctgga atcatttctg
     6061 tcttcgttta ttgctttatg gtttcgtgga atgattattt atttgcttcg attttcctgt
     6121 cgacctccga aaaatttact ttaccaatcg gtttgaacac attattcagt acaccagact
     6181 acatttgggg acgaatgatg gccgcttcat tggtgaccgc tttaccagtc atcattatgt
     6241 atgcgttatc tgaacgattt attaaaggtg atttagtcga aggcggcgta aaaggataaa
     6301 aagaaaagaa agaggaaaga aaagatgaaa aaattagttg cattagaacc aagaatagcc
     6361 ggattcaaag attacgaaga ccgtgagatc gaaaagaacg aagtacgtat ccgagtaatt
     6421 ttcgctactc caaaaccagg aacagaaatt gtcgacttta gaggattaag tccatttatc
     6481 gatgaagaat tttcagaaga atggcgccta ttcttgccaa gagaagaagg aagccaacgc
     6541 ggtgtggtat ttggcgaatt tcaattagga aatatgattg tcggtacgat cgaagaaacg
     6601 ggcagtttgg tgacagagta tcaaaaagga gagttagtct gcacttatgg acccattcgt
     6661 gaaacgctga ttacaaacgg tgttgataat tataaactac gtaaacttga tcggttggat
     6721 caatggaaaa atgcagtatg ttacgatcct gctcaattcg ctttaggtgg tatccgggaa
     6781 gcacatgttc gagcaggtga tgctgtagca atcttcggtt taggcgctat cgggcaaatt
     6841 gcgattcaat tagctaaaaa agcaggtgct actaccatcg ttgggatcga tcccatagcg
     6901 cagcgtagag aattagcaga aaaatacggt gccgattaca cgctagatcc tatgaaagca
     6961 gatgtcggtt ttcgtctaaa ggaattgaca gataaacgtg gagtagattc agttattgaa
     7021 acgagtggca gtgctgccgc attacaagct tctttaagag ggatcggcta tggcgggatc
     7081 atttcttatg ttgcctttgc taaacctttt ccagctgggt ttaatcttgg tcgcgaagca
     7141 catttcaata atgcaaaaat cgtctttgct cgagcatcta gtgaaccaaa cccagattac
     7201 ccaagatgga accgtaaacg aatcgaggac acatgttggg aattattgat gaacggttac
     7261 ttggattgtg aagaaatcat tgatccagtt gtgccatttg cagaaagcgc agaagcttac
     7321 atgcgttatg tagaccagca cccagaagaa agtatcaaaa tgggcgttca agtccgagga
     7381 ggagaacaat gaaattcggt acgcaagatc aagcattttt cccaagagac attttagaaa
     7441 aatttagatt tatcaaagac tgtggatttg aagaatacga aatcgacggt aagttgttaa
     7501 tagaaaatat cgacacagtc aaacaggcaa tcaaagaaac agggatccca gtagtgacag
     7561 cttgtaacgg ctacgatggg tggatcggtg actttatcga agaacggcgt cttaatggtg
     7621 tacgtcaaat tgctgagatt ttgcgtgcac taagcgaggt tggaggcaaa ggaatcgtta
     7681 tcccagcagc ttggggaatg ttcacttatc gattaccgcc gatggtttca ccccgaagca
     7741 aagaaggaga ctataaagca gtcagcgaat cacttcgtta tttagataaa gtagccgaag
     7801 aaacaggtac ggttatttat ttggagcctt tgaaccgata ccaagaccat atgatcaatt
     7861 tgttagcgga tgcgcgtcat tacattgact ctaatcaatt gaaacatgtg cagatcatcg
     7921 ctgattttta tcatatgaat attgaagaag atgatatttc aaaagcattg actacgcaaa
     7981 aagagtatgt gggtcatatc catttagcag ataatcatcg ctaccaacca ggatctggcg
     8041 caattgattt tactcgccat tttcaaacgc tacttcaaaa tgagtatgaa ggttatatgg
     8101 tatatgaatg tcgtttgcga ggagaaaatg aagtgaaaat ttatcaggaa tcattgcaat
     8161 acttgaaaaa ctgtctggaa acagcgcgtc atttaaatga tttagagaaa gaatagagaa
     8221 agaataacaa taagtaaata agcaaaggag ggagaaataa atgaagaaag tagcaatcat
     8281 aggcgctgga caagtagcag aaaaagtaca tgctgcttat tatcaaaaaa gatcagatgt
     8341 cttagaattg gccgctgtgg ttgatccagt cagccagcga gcagaagact tttgccgacg
     8401 tacaggattt tctcatgcgt atacatcagt tacacaaatg ctgaatgaga tttctcccga
     8461 tatcgtgagt gtctgtacac ccaatcgctt tcacttcgaa catgtattgg ctgccctcga
     8521 agctaatgct tcagtgatgt gcgagaaacc accagctatg acagctgatc aagcaagaaa
     8581 gatggcagag acagccgata aaaacgggca gattttggct tatggattcc agcatcgttt
     8641 ttctgacgaa gcaacaatga tcaagaatca tcttgatgtt ctcggggata tttattacgt
     8701 agaagcgaat gcgcttcgtc ggtctggtgt tccaggctgg ggaacgttta tcgacaaaga
     8761 acttcaagga ggcgggcctt tgatcgatta tggcattcac atgcttgata cagcgatgta
     8821 tattactgga tttcctaaag taaaaagcgt tcgggcgtac agcttccaaa aaattggtac
     8881 gaaaaaaaat tcagggaaat tcgggaaatg ggatccaagc aaattcacag tagaagatgc
     8941 aatttttggc acactggaat tagaaaacgg caagatcatc caactgaata cttcgtttgc
     9001 tttgaatatc agagaagaca aactgaatgt ggctttttgt ggcgatcagg caggtgctaa
     9061 tctgtatcca ggcgaactat tcgaagatgt agatggaaag ctggtttcct tacaaaagac
     9121 cacgccagtt aatgacccac atgaactaag tatggctcat tttgtgaatc gggcgctcgg
     9181 tgaccagact cagatgattg cagatggctg gcaaggatat gtcatccaga atctggtgga
     9241 aaatttatat cgttcggcag aaacaggaga agttattacc ttatgaaagt agaagaaaaa
     9301 cagtttgatt tagagtatat caataagtat gcgagtcaga tgaccattgg aaatggattt
     9361 tttggtatca gaggaagcca ggaggaagac tatcggaatc aagtaagggg aatgtttgca
     9421 gctggtgtat ataaccgacc tttaggagca gaatcagccg agttagtgaa tctcccagat
     9481 gtcatcagat gggaaataaa aatcgacggt gaacattttt ctcttgagac gatgaaagtt
     9541 cttgcatacg agcgggcact agatttagaa atgggagagc ttaaacgcta tgcgctcatt
     9601 gaaactgctc aaggaaaaaa agtggaagtc attacttgtc gtgttgctga tcaaaataat
     9661 ctccatcagg cagccctgaa agtggtcatc aagccgttga ccgaagactg ccaagtagat
     9721 gtgaaaacgg gtatcgatgg acagcaaacc aattttggta cacagcagtt ggttgaaaaa
     9781 gaactgcgag tatttggtga cgatttgatc agtgcatcct accagacaac agaaagtaaa
     9841 attgatattg ggattgcagc taaattcaat aaagcaggtg tattccaagc aaaaaatcgc
     9901 cggatcaccg cttccttttc agaaaagatc agaaaaaata cgtcctttac gctggagaag
     9961 atcatcgtgc tctctacctc tcttcagcaa acagacccgt ttaagaaaag tattgcccaa
    10021 gcccagaaaa gcgttagtta tacagaaatt ttagagcagt cgatgtctta ttggacagat
    10081 ttttggcatc agcatcggat aacgatccaa ggcgattctt ctttcgatca gttagcgatg
    10141 gacttcgctg cttaccattt acagattatg actccccgca atgatccaag tgttagtgta
    10201 ggggcgaagg gactaactgg cgaaggttat aaagggcatg ttttctggga tacggaaatt
    10261 tttatcctgc ctttcttttt atatcagttt cctgaagtag caaagaattt gctgctgtac
    10321 cgttatgatc gattagaagg cgcacggcaa aaagcaagag attatcacta tcaaggtgca
    10381 cttttcccat gggaaagcgc acacactggt caggaagaga cacccaaatt tgctgcattg
    10441 aatatcaaaa ccggaacaag acaagaagtt gcttcagctg ccgcagaaca tcatatcagt
    10501 gcagaccttg cttatgccgt tcaagagtat gtccaagcaa cagaggatca atcgttttta
    10561 gaaacaaagg gaaaaacgct cattcatgaa acagcagctt tttggctgag cagagctgta
    10621 aaagtcgatg atcatttaga gatacacaga gtcattggtc cagatgaata cacagaatat
    10681 atcgacaata atgcatatac gaactatctg gcgcattata atgtggcctt agctgttaaa
    10741 tatcaagctg gcgatccgct ttttttgaaa gaatgtcaaa attttttaga taagttatat
    10801 ttgccagtgc ctaacaaaga acagctgatt ccgcaagatg acacgtatct cagtaagcca
    10861 gttattgatt tgaccactta caaagaacgt cctggcagcc agtctatttt acttgattat
    10921 gcccgtagcg aagtaaatga actccaagtc agcaagcaag cagatcttgt catgctgtta
    10981 tacttattac ctgagtattt ttctcaacca atcgtttcta agaatttgca ttactatgaa
    11041 aacaaaacaa tccacgactc atcgttgagt aaagcgattc acgccataga ggcttgccgt
    11101 atccaagaaa tggaatgggc gtatcattta ttccaagaag cgtgcctgat tgatttagga
    11161 ccagatccgc attcatcaga tgacggaatt catgctgctt cattaggagc actatggcta
    11221 gcagttatat ttggtttttc aggtattaca gtaacgaatg gaactctttc tatttctcca
    11281 agacttcctt cagcatggga aaaaatttct tttcctttta tttggaaaaa tcacaggttg
    11341 ctatttgttt tgacaaaaaa acaaatagta ataaaaaaag agacgaaaga aacattacct
    11401 atttttgtca atggagacca ataccagctg gaaaaagaac tgatcttgga acggagggga
    11461 aaataatgcg tattctgatt gcgccagatt ctttcaaaga aagtctgaca gccttagagg
    11521 cagcacaagc cattcaatca ggttttcaag aagtttttgt tcaggctgaa tatgatctgc
    11581 tacctatagg cgatggagga gaaggaacgg tttcatcatt agcagacagt ttaaggttga
    11641 agcggcaaac agcagtcgtt actaatgcat ttggcaaaaa caaagaaatc cagtacgctt
    11701 cagacggtca aactgcgatt tttgaaatgg cagaaatcgt cggattgaaa gatgtcccac
    11761 taaaccaaca acagccactt aggttatcca cgcgaggagt aggagaactg atacgacaat
    11821 tgttgcaaca aggtgttcga cacatcatca tcggcgttgg cggaagtagt accaacgatg
    11881 gcggcatcgg catggcagaa ggtctaggtt atgcttttta cgatcaagat aatcaaaaag
    11941 tagaagcact gggagaaaat ctaggtaaaa ttgctacagt tgataaaacg aacgtgccta
    12001 ttgagctgat taaagccaaa atcactgtca ttactgatgt tactaatctt ttatgcggaa
    12061 aagaaggcgc ttcttttgtt ttcggaccac aaaaaggctt gaaagaaaat gaattagcag
    12121 tggctgacca agccatgcat gatttttata tgagatttgc ccctcaagta atcgaggcat
    12181 cgggcagtag tgctggtggt ggaatgggtg caggactgtt agcatttgct tcagggaaaa
    12241 tagtctctgg catcgactac gtattggatt tactggagtt cgataaacgg gtacagcaag
    12301 cagacctcgt aatcgtaggc gaaggacgtc tagatggaca aagtctaaaa ggaaaagcgc
    12361 cagttgggat tgctagaaga gtaccagagg gcgttcctgt gatagctatt tgcggaagtg
    12421 ttggtaaagg aagcgaaaaa gcagcagatg ttggcatttc ggccgttttt ccgatccttc
    12481 ctgcagctgt gcctttacaa gaagctttac agcaagccac tgataatctt catcgcacag
    12541 ctaaaaacat cgcatcttta cttcaagtcg ttcattttca aggaggtaat cgatgaaaat
    12601 caaagcagca ctattcgatt tagatggcgt tcttgtagac acagctcgtt atcattacga
    12661 agcgtggctg gttttagcta accagctgtc cattccattc actgagaaag aaaacgagca
    12721 actgaaagga atatccagaa ccgagtcact agagcgattg ctgtcatttg gaaaaatgga
    12781 acagaaattc tcagaaaaag agaaatcagc ctttgctgag caaaaaaaca atctatatct
    12841 tcaagcgatt caaaaaatgg atgaaacatc cgttttgcct ggtgcaatag ctgttcttga
    12901 gtacttgaaa aaaacaaaca tcaagatcgg attgggatca gcaagcaaaa atgcacgcct
    12961 cattttagaa aaaacgaatt tgacaagtta ttttgatgtc cttattgacg gcacgcaagt
    13021 atcaaaagca aaacctgatc cagaagtttt tctcaaagga gcacagcaat tgaatgtacc
    13081 gccaaatgca tgcttggtca ttgaagattc agaagctggt tgtcaagcag ctttggctgg
    13141 taacatgcat gtattgggaa taggtgaaaa cataaatctt ccttctgctg aatatgtgat
    13201 tcctgattta accgtttttg atcaagtacg ttcgttttgg catttatcag aagccgtatc
    13261 acgtatataa tagtttccgt tttttctgtc acaagcaaaa cgttatcctc cttttggaaa
    13321 tcaaggtatg atgagaaaaa agggggattt tatatgacaa aaaaagcagt tttcgcaggc
    13381 ggatgctttt ggtgtatggt caaaccattt gaagagcagc cagggatcat ttctgtgaca
    13441 tccggttata ctggcgggca tgtaccaaat ccaacttatg aacaagtaac aacaggcaca
    13501 acagggcata cagaagcagt agagatcgaa tatgatccag aagttatttc ttatgatcag
    13561 ttggtggaaa tctattggca gcaaaccgat ccgactgacg cattaggaca gttcgctgat
    13621 cgcggtgatt cttatcgtcc agtgatctac tataatgatg aagaagaacg aaatattgca
    13681 gagcgttcaa aacaagcatt gcaagacagc gggcgattcg ataagccaat cgtgacaaaa
    13741 atcgaaccaa gaagcgaatt ttatcctgca gaagattacc atcaggatta ttacaagaaa
    13801 aacaagctcc attacaatct ttatcgagaa ggttctggac gtgcaggttt ccttcgtcga
    13861 cattggggcg tcaagaaata agaagccgaa aaagctactt atggaacacc gtatatcaga
    13921 ggttgtaaaa ccagcatttt gagctaatac tgagggtcag ctgattgaac accatttagt
    13981 ggagaggttg tgaacctgtc gcctagctcc aagtcacaag gatttggtaa gagaacacta
    14041 tatatcagag gttgtgacaa gtcgtttgtc acaacctttt ttcaatacct aagatgaagg
    14101 acaatcagca acgcactgct caaaaaagga atgaaaggca gcttttccaa ctttttttta
    14161 tgacagataa agtaaacaga aaaaacggat attccaaaca gactagcaag aaacaagatc
    14221 tgaagcaact cttctagaga caagccacca ctccaagaaa gcatcaacag agtatcgcca
    14281 gctccgagat aggattgacc atagcgaagg cacacaccgc ctacgataaa cgttaacagc
    14341 acactttgcc attggaagac gccggcctgc aaccaaaaaa tccataaaat tgcccaagaa
    14401 cagaatagcg tgtgagaatg gaccatgaag taaaataaat ccatcaacga aaatagcaaa
    14461 gccgaaagca tccatatgaa cgtcgaaaaa tttcttgcgg aagaaaaagt aaagaaccag
    14521 gcaaaaagac caccacataa ggcttctgcc aacaaatagt agaagggcaa ccgacacctg
    14581 caatagcggc agcgaaatcg ttggacgatg atcgagagaa tcggaatcaa ttcataccaa
    14641 gaaagcgaac gacagcattt gacacaatga gatctaggat aaataataga gtgacctaac
    14701 ggaatccgct gggcggttaa acataaaaat gatccgatac agcaaccaat gataaaataa
    14761 agtaacacgc catctccccc ttttcaaaac caagatacgc aagcttaaac taaatttttg
    14821 tacatacata aaatttgagg tgaaaaaatg aacgtttcaa tcggattaga tatcggaacc
    14881 acacaaacca aagcagttgc tttcacagat gaagcccaag aagcagcatc cgcttatttt
    14941 cgctaccctt tgatccaaga aacacaaggg atggcagaac aagatcagga attgatcttt
    15001 caaggtgtct gcaccgttat aaaagaagta attaaacagt tgccttcaga cacaatcatt
    15061 caatttgtct ccttctcctc tgcgatgcat ggcctgatat tgttggatga agagaaaaca
    15121 ccgttatctc gaatgatcac ttgggcagat aaccgagcag cggacgaagc agaaaaactg
    15181 aaatataccg tgcttggcaa agaattttat cggcagactg ggacgccgat tcatccaatg
    15241 acaccattga acaaaatcaa gtggctgcaa aacagccaac ctgaattatt caaaaaaact
    15301 gcttattttg tcggtatcaa agactatatc ttttatagat tgtttggcga actgatcagc
    15361 gactacggaa cagcttcagg aactggatat tttgatatcc atgaattcca atgggcagaa
    15421 gaagtgcttg actacttgaa cattcgaata gagcaattgc cgcaagtctt tccttccacc
    15481 tatcaagtaa caggtctacc atcaaaaaca gcagcagaac taggtttgcc aaatgatata
    15541 tcatttgttt tgggaggggc agacgggccg ctttctaatc tagggttagg tgcttttggt
    15601 gatacgatcg ccgctttgac cgtaggaacg agcggagcac ttcgttttat tactactcag
    15661 cctcagttcc atccagaaat ggagacgttt tgttatgttc tggatcagaa acattgggta
    15721 gtgggaggag cgactagcaa tggtgctggc atctttgatt gggcagggca tacattttta
    15781 caggaagtcg ttcaatctgc taaagaaaaa ggagaaaatc catatgatgc actgctttcg
    15841 gctatcgctt ttgtgccagc gggtgccaat ggtctgattt ttcatcccta tttacttggt
    15901 gagcgagcac cgctttggga cgcagatgcg tttggcagtt ttatcggttt acagcggcaa
    15961 catacagaaa aagaaatgat gaaggcgatt ctagaaggga tctgtctcaa cctttatcgg
    16021 attttaaaag gagtttgcca ggaaaatcag ctcacagaga tccgtgctac aggcgggttt
    16081 gctcaatctc cgatttttcg tcagatgatg gctgatgtac tgggttaccc gcttgttttc
    16141 cctactgtca cagaagcatc cgcattagga gctgtgattt taggctggag aagcatcgga
    16201 aagatccaaa gtatcagtga agcacaggaa ttgatagaat tgaaagagca agttggagta
    16261 caagaaactg atcataaaaa atatcagaaa atctatccat tatttgtttc gacacaaaag
    16321 catttagcac agacttatca gcaatttgct caactgcgag aagaattagc tgattctgaa
    16381 aaatgattgg ctttacggat aaaaaagcga actgaaaatc gttctcaggt aaatgatttt
    16441 gttgtacttt tcattgaaag tctactatta ttttaagtgt aagaaaatgt taggaggcaa
    16501 gaaagatgaa attccaacaa accaaagaag tattaaatca attagttgca gatctaagcc
    16561 aaatgtctgt tgttgttcac caaacacatt ggtacatgag aggaccaggt ttccttacat
    16621 tgcacccaat gatggatgag ttcatggatg atttgaacgc tcagttagat gaagtatccg
    16681 aacgcttgat cacattagac ggttcacctt attctacatt gcgtgaattt gcagataaca
    16741 caaaaatccc tgatgaaatt ggccgctggg atcgtactat tccagaacgt ttggaaacac
    16801 ttgttgcagg ctatcgttat ctagcagatc tttaccaaaa aggaatcgaa gtttctggag
    16861 aagaaggcga cgatccaaca caagatatct ttatcgaatt caaaacagcc acagaaaaac
    16921 gtatctggat gatccaagct catttgggca aagcgccaga gatcgacgcg taaaaactgt
    16981 tgttgaactt gaccgaaaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
    17041 acaaacgtca aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
    17101 tgattgaaca ccgtttacca agaggttgta agcctgccgt tcagctccga gtcacaagaa
    17161 acaattttaa aaaacagctt cttatgtttt atggatattg tgacttgtga ccgaggagtt
    17221 ggcaggagaa caccgtttat tcaaaataag gggttgtgac aagacttttg tcacagcctc
    17281 tttttgtttt tcattttgac aggaaacgac ccttttatat aaaagaaaga aaagaagtaa
    17341 agtttcatat aaatttttca atcaagcaga aaaacgttga cattgactag attagcgggt
    17401 aatatgttaa atggtattgt ttgccccaca aagagccccg gaaactcgaa gtgtatgtca
    17461 aacatcagaa cttaaattgg aggaaaataa cgtgcgtaca acatatatgg ccaaaccagg
    17521 cgaagtagaa cgtaaatggt atgtagtaga cgcaactgat gttccattgg gacgtctttc
    17581 aacagttgtt gcatctgtac tacgtggtaa aaataaacca actttcacac ctcatttgga
    17641 tactggcgat ttcgtcatcg tcatcaatgc agacaaagtg aaattaacag gtaaaaaagc
    17701 gactgacaaa atttattacc gtcacagcaa ctacccagga ggattgaaat caatcactgc
    17761 tggcgaattg cgtgctaaaa attctcgtcg tttgatcgaa acttctgtaa aaggtatgct
    17821 tcctaaaaac actttaggcc gcaaacaatt tacaaaattg aacgtatacg gtggagcaga
    17881 acatccacat gcagcacaac aaccagaagt tttagacatc acaaacttaa tctaatagga
    17941 gggaatttca ttggcacaag ttcaatatat cggcacaggc cgtcgtaaaa acgcagttgc
    18001 tcgcgtacgt ctagtaccag gaactggcaa aattactgta aataaaaaag atgttgaaga
    18061 atacattcca catgctgact tgcgtgaagt aatcaatcaa cctttcgctg ttactgaaac
    18121 aaaaggcgca tatgacgtat ttgttaacgt aaacggcgga ggatacgctg gacaatcagg
    18181 agctatccgt catggtatcg ctcgtgcatt gctagaagtt gatcctgact tccgtttagc
    18241 tcttaaacgt gctggcttac ttactcgtga cgcacgtatg gtcgaacgta aaaaaccagg
    18301 tcttaagaaa gcacgtaaag cttcacaatt ctcaaaacgt taatatcctt tatatatcaa
    18361 cgtttctggc acttttcaat tatttggaaa gtgcttcttt tgtttttaag aacacattta
    18421 agaacacata tcaatcacac ttattcaatc ctctctgaaa agcttcaaaa acagccttat
    18481 caactatatc tggtgtgttc acatatatct ttgttgtaga aatatctgag tgtgttaaag
    18541 catttgaaat ttgattcata tctgctccac cttgtctagc tatagttgca taagtatgac
    18601 ggagtttgtg tggagataaa tgaactaagt caggatgcct tcttttaatt gaattgattc
    18661 gatagtttag gtaatcggca tgaacaggac aatttacttc tccagatggt ttagtataag
    18721 taaagagata ctgatctaac gtttgcttaa tttttaattt caacaattga tcagcttgta
    18781 ccgatttcca ttcacttagc aatgtcatta caacttctgg tacttgaatg acggtatctt
    18841 ttcgaccttt tgtaaacttt tttctttgat atttatctaa agtatgttta agacggaccg
    18901 tttgtttttc aaaatcaata tatttccatt gcaaagcgta agtctcactt tttctatcac
    18961 caagatacag ggtaatcata aaaagtgtgt aatcatgaaa agtaagtaaa tgattattaa
    19021 aatcatcatt tactgcttct atccaattag taagttcttt tgatgatagt gcttgcttac
    19081 cttcttgtat tcttttttct tctaaagcat tttttttagg tgcagtgata ctttgaataa
    19141 ttttagtagt tcgattaaat tcgatataat ttagtatttc tgcaatatcg aacatggaac
    19201 gaacatatcc ttttatgatt tttatatttg catattcttt cgattttttg gttaattcat
    19261 ctgatattat ctcagtattg atatttagat aattcattgc gtattcacca aacataggta
    19321 aaatatgcaa ccgaaatata tcttttgtat tactaatggt tatatcacta ggaggcctgt
    19381 ttgatctaat tgtttgacct aattcatatc taggaagcca tttagattcg taaaattttt
    19441 taaacgttat ttttcctttt aattctaatg agtttgcatt ctcctctctc aaaacttttt
    19501 ctattttctt cttcatatca ttttcggctt taatcgccaa ttgcctattt ggaaatgttt
    19561 tttgatactt ttctgatgca attcctgtta tttctttaaa cctttttgga tatctcagat
    19621 tagttttata tttcccattt gaaagctttg taggcaaaat cctcactcct taaaaaaagt
    19681 aaggcagtat atttcactaa ttttagtata tgcggcgaga agaaaatttc aagttatttt
    19741 aaatgtattc ataaagtctt taatatcttc tacgtcaaag cgaactacac caccaactct
    19801 tattactggg aaattatata tacgaatcca cttatctaga gtattattat cgattcctag
    19861 aaatgcacag gtttgttttt tattcaggta gactctggat tctataaaat catctcctaa
    19921 aatagtagtt atgaaatttt tgaattgctt tgggtaagca cataggaatt tcacctctcc
    19981 ccagtttacg gcgagcctta cggaagtatc attatttgct gctattgtca tggcattttg
    20041 ttttcccaga acaaaaagaa atctctaagt atttgtcgct aatcaggtca tggcgtactt
    20101 gagtttctag ctcgaatagc agggatcgtt attcaaaaat tgtatttaat tgtcaatgat
    20161 caaaaatagt ttttttccta ttcactatta ataacgataa acatgtataa aaagacacac
    20221 tacgaaaaaa gttaaatgga gtttactgat tttcatattt gtatatggaa cagacatagt
    20281 cttgaataat ttttataaat gttttgatgc ttatcaactt attcaacccg ctatccactt
    20341 caagtataat ttttccatac gccaataaag tagttttagg agcatgttct catttgagaa
    20401 cgctcctttt ttatatattt tgaaagctac ttactatacg cgtagtttca aatagttttg
    20461 tcattctaat cttcagcaac agaaaaaaat ctaaactata aaatttaggg ttgactaaaa
    20521 tataattttt gtataccata tgatcgtgat aatcattatc atttagaagg agaaagttat
    20581 gttagaagtg aaaaagctta atgtttgtta taacgatttt ttagcattga acgagatttc
    20641 tttaaaaata caagaaggtg ctttaacagg tattattggt ccaaatggtg ctggaaaatc
    20701 cacattgctt aaagcaatgt taaatattat ccctcatcag ggagaggtct ccattaataa
    20761 tgaagatatc aataaaaaac tggtgaagat tgcatatgta gagcaaaaag ctgatattga
    20821 ttttactttt ccaataaaag taaaagaatg tgtatctatg ggaacatatg ccggaatgaa
    20881 agtttttcaa agaattaaaa atgcagaatg gcaaagagtt tcaaaggctt tggaaaaggt
    20941 cgatatggga aaatattcta atcatcaaat aggagaatta tctggaggac aatttcaacg
    21001 tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc atatttttag atgaaccatt
    21061 tgtagggata gacttagtta gtgaacgaat aattatggat acgttaaaaa aattgaaaca
    21121 tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc aaggtaaaga agtattttga
    21181 taatattatt cttttgaatc gaaatctaat tgctcacgga agtgtagaat ctgtatttaa
    21241 tgaagaaaat ttaaaaaaag cctatggaga tactatattt attggagagg gggaataata
    21301 tgcttcaaaa ttttattaat ggtctgtatg actttcactt tttacagaat gctctaatta
    21361 catctgtagt tatagggatt gtttctggaa cagtaggttg ttttattatt ttaagaggaa
    21421 tgtctcttat gggtgatgct atttctcatg ctgttttgcc aggagtagca a
//
LOCUS       DAZUKA010000044        20372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000044 DAZUKA010000000
VERSION     DAZUKA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.044"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..777
                     /locus_tag="ACG8PN_001249"
                     /pseudo
     CDS             212..777
                     /locus_tag="ACG8PN_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1476..1997)
                     /locus_tag="ACG8PN_001250"
     CDS             complement(1476..1997)
                     /locus_tag="ACG8PN_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4861414.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            2195..5875
                     /gene="nifJ"
                     /locus_tag="ACG8PN_001251"
     CDS             2195..5875
                     /gene="nifJ"
                     /locus_tag="ACG8PN_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4861415.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            6064..6951
                     /gene="cdaA"
                     /locus_tag="ACG8PN_001252"
     CDS             6064..6951
                     /gene="cdaA"
                     /locus_tag="ACG8PN_001252"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4861416.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            6948..8117
                     /locus_tag="ACG8PN_001253"
     CDS             6948..8117
                     /locus_tag="ACG8PN_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4861417.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            8141..9496
                     /gene="glmM"
                     /locus_tag="ACG8PN_001254"
     CDS             8141..9496
                     /gene="glmM"
                     /locus_tag="ACG8PN_001254"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4861418.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(9568..11373)
                     /gene="glmS"
                     /locus_tag="ACG8PN_001255"
     CDS             complement(9568..11373)
                     /gene="glmS"
                     /locus_tag="ACG8PN_001255"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4861419.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            11783..12073
                     /locus_tag="ACG8PN_001256"
     CDS             11783..12073
                     /locus_tag="ACG8PN_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861420.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12086..13111
                     /gene="sppA"
                     /locus_tag="ACG8PN_001257"
     CDS             12086..13111
                     /gene="sppA"
                     /locus_tag="ACG8PN_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4861421.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            13114..13773
                     /locus_tag="ACG8PN_001258"
     CDS             13114..13773
                     /locus_tag="ACG8PN_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4861422.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            13930..14862
                     /locus_tag="ACG8PN_001259"
     CDS             13930..14862
                     /locus_tag="ACG8PN_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861423.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            15016..16305
                     /gene="tig"
                     /locus_tag="ACG8PN_001260"
     CDS             15016..16305
                     /gene="tig"
                     /locus_tag="ACG8PN_001260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4861424.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            16552..17802
                     /gene="clpX"
                     /locus_tag="ACG8PN_001261"
     CDS             16552..17802
                     /gene="clpX"
                     /locus_tag="ACG8PN_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4861425.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            17885..18472
                     /gene="yihA"
                     /locus_tag="ACG8PN_001262"
     CDS             17885..18472
                     /gene="yihA"
                     /locus_tag="ACG8PN_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4861426.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18546..18701)
                     /locus_tag="ACG8PN_001263"
     CDS             complement(18546..18701)
                     /locus_tag="ACG8PN_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4861427.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            18837..20165
                     /locus_tag="ACG8PN_001264"
     CDS             18837..20165
                     /locus_tag="ACG8PN_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861428.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            20176..>20372
                     /locus_tag="ACG8PN_001265"
     CDS             20176..>20372
                     /locus_tag="ACG8PN_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861429.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSE"
BASE COUNT         6695 a         3540 c         4405 g         5732 t
ORIGIN      
        1 tctgaaccga ttgttaacaa ttttggtgat tagaaaacaa ttacgataaa tagaaacaag
       61 ccaaatcatc agcaagtaaa agctgatgat ttggcctttc ttatacgagt agtaggaatt
      121 catagcgaat attattacga tcagtagata ttttgatatt aaaggagtat tacgaactag
      181 tatgtgagaa tgtagaggtg atttgcgaat aatgagaaat ttatcaaata gaaacaaaat
      241 attaatcatt attgttgtta tagctgtgtt tcatctggga acgaatgcgg ttttaagtag
      301 aataatattg ggtcctaagc caccaagacc tgcgattacc cgaggggagt ttgattttag
      361 attagaatat gaagtagatg gggaaaggat tgtgatagaa gatacaattg ttgcattatt
      421 tgatggattt tctgcagatg caggtagcat ggcatggtat cgcacgtggc gtttgcattt
      481 ggcgagtgac aagagggcac aacaattatt gttacatgag ttagatgatg gtcgaaaaat
      541 atattatcat tttggaggag ctggatttta tatggggaag ttagtagcag tgtaactgta
      601 acaaggaaag atttgccagg tcttggcatt cgaaactcca tattttatat cgagcgtggg
      661 agagatacat ctattgggaa caggaaaggg ttaactcttg aagagttata taatgacttt
      721 ggaatccgtc ttgttagttg ggaacacaat ccaccaatag aaaatcgctt tgaataatcg
      781 aaatctatta atccatttca tcagccagtg aatgttgtaa tggaacaatt tggtttagtg
      841 ttcaaaacca ttgaaatact aggctgtgat cagcaaaaat gtatctgaat aaaaaagaga
      901 gcaactatta aatgagagga gttaggagtt gttagcttta tacgaatcaa agtggcaaat
      961 acagatgcgt atgcatttgt gtttgccatc ttgcgtttgg tagacgagat aggtataagc
     1021 taaagaaaca acttgtttaa aataaacgac ctagctcaac aaacgaccat ctaatcaact
     1081 aacagcatac ataaaagcaa ctatttttga agtagaattg aaatcgggat aacttaaaaa
     1141 gtgattggga tactagtgtg aatcaccgta cataaatatt actcgttaag ggaatttgag
     1201 aaattttgta tgaaaattta agaattagtt agtatgcgca agtcaaaaat atgaataaat
     1261 tatttggtgg agataatttg ctcagcttgt cataagaaat gagataatct ggcagatgca
     1321 agcttttgga caccatttat tcggaataag gaggctggga cagaagcgtt taactccaag
     1381 aaataagaag gagttgcttc tgcttccgcc gtttataggt gtttagagcg tgagacaaaa
     1441 gtgttcttta cttttgtcct acgctcctta tttttttatt ttgtttggat caattctttt
     1501 aattcctcta ttgaaataga ttcatcaaag atttctttac ccaagatgac agtcggaaca
     1561 aatcgaatat gtgcttgctc agcttcgttg atgacagctt ggctcagctg ttcgtctttt
     1621 tgttctgtca gctttagttt atctactgca aaatccgcta cttcttggag agaaagatgt
     1681 ttccactcgt cttgagtatc gaatatttgt ttgatctctt tgatcgcttt ttgaccatcg
     1741 ctgattgtga gataccggtg catcacgttt cctcgctgca agctttcttt ttctttatcg
     1801 aacaatttga tcactcgctg caattgcccg ctttggactg cttcttctaa tagctcataa
     1861 gattcttcaa accattgtcg gcaatacgga caagataggt tgatgaattc gaccattttt
     1921 ttaggagcat tcgatgaacc ataaaggatt cctttttgtg tatttgtttt cgttgcatca
     1981 atcactgata tatccatgtt aaaacacctc tttcgcttat agtttataca tgtctgacaa
     2041 aaaaaggcaa cgatttagac tgtttgtcta aaaattagat gaccaattca aatagacata
     2101 tgtgaaatat ttttataaat aaaagaaaaa atggaatgtt ttacgttata atggaacaga
     2161 taataagaga gaatatgaac aaggatgatg aatcatgcga aaaatgaaaa caatggatgg
     2221 caatacggca gccgcatata tttcttatgc atttaccgag attgctgcaa tatatccaat
     2281 aacacccagt tcgaccatgg ctgaattagt agatcaatgg tcagcagcag gaaaagaaaa
     2341 tgtattcggt caaccagtaa aagtagtaga aatgcaatcc gaagcaggag cagctggtgt
     2401 ggtccatgga tcattgaaaa ctggtgcact gacaactaca tataccgctt ctcaagggct
     2461 gcttttgatg ataccgaata tgtacaagat tgctggggaa ctactgccaa gtgttttcca
     2521 tgttgctagt cgggcagtga cgacaaatgc gttgaatatt ttcggagatc acggagacgt
     2581 gatggctgca agacaaacag gatttgctat gttagctgaa agcagtgtac aagaagtgat
     2641 ggatttagca cctgttgccc atcttgcttc tattgaagga gaagtacctt ttttgaattt
     2701 ctttgatgga ttccgtacga gtcacgagat tcaaaaaatc gaagttttgg aatatgaaga
     2761 attagctcct ctgatcaatc aggagaaatt agcttcattt cgtaaacgag ggatgaatcc
     2821 caatcatccg actgttagtg gtacgaacca gaatccagac atccattttc aacaacggga
     2881 aacgatcaac ccctattatg ataaattacc tgaaatcgtt caaaaatata tgaaagagat
     2941 caaccgatta cgaggaactt cttatgactt ggtgacttat tacggagcag acgatgcgga
     3001 agaagtcatt gtttctatgg gatcagttgc acagacgatt gaacaaacga ttgattactt
     3061 gaatgcaaac ggacggaaaa caggtttttt gaatatccat ctttatcgtc cttttccagt
     3121 ggaaacattc gtgcaaaagt tgcctcatac agtaaaaaca attgctgttc ttgatcgaag
     3181 taaagaacca ggggcaggtg gagaacctct cttattagac gttcaaagtg cgttatatga
     3241 ttctgaactt cgcccggctg ttattggtgg tcgctacgga ttggggtcta aagatgtgac
     3301 tcccgaccaa atctcagcag tttttgatga attgaaaaaa gacccaagca tacgcaaaaa
     3361 acggtttacc atagggatcg tggatgatgt gacttatcaa tctttggaac caaaggaaag
     3421 tctggatttg acagaaccgc aaacattcca ggccaagttt tggggatttg gatctgacgg
     3481 aacagttgga gcaaataaat cagctatcaa gattattgga gatcacaccg ataaatatgc
     3541 gcaaggctat ttttattatg attcgaaaaa atctggcggt ctgactgtct ctcatcttcg
     3601 ttttggagat acgccgatac gttcagcgta tcttgtcgag catgcggacc ttgttgcctg
     3661 tcacacacca gcttacctgc atagctatga cttggtcaaa ggattgaagc caggtggcat
     3721 ttttttattg aatactcttt ggagcgacga acaattagaa acgcatctgc ccttaaaatt
     3781 aaaacgttac ttggctgaaa ataacattag attctatacg atcaatgcca tgagattggc
     3841 ccaagaagtc ggtttgggac gccggatcaa tacggcaatg gaaacggctt tttttaaatt
     3901 agctgacatc attccttttg atgaggtgct tccgctgcta aaagaagaag ccctgaaaag
     3961 ctatggacat aaatcaatgg ctatcgtaga aaaaaacata caagcaatcg atcggacgac
     4021 cgaactgctt catcaagtcg cgatcccaga ccattggaga acgttggaga tgcctgaaaa
     4081 aatcaataaa aggaacactg gttatataca agaaatactt gaaccagtga atgcacagga
     4141 aggaaatcaa ctctctgttg gtacacttgt agcaaatgga atgacaactg gcgaaatgcc
     4201 tttaggaacg gcttctatgg aaaagcgggg cgtcgcttta gaagtacctg agtggattag
     4261 tgatcgctgt acgatgtgta atgaatgtgc ctttgtttgt cctcatgctg ccatccgtcc
     4321 atttttagca gatgaagagg aaatggaaga agcgccagaa ggatttatcg tgagagaaat
     4381 gcgaggcgca gacggcgtaa aataccgtat ccaagtttca gtggaagact gtacaggttg
     4441 tggtctgtgt gtagaggctt gtcccgcaaa agaaaaagct ttagtgatga agccttatga
     4501 agaacagaaa gaacaagctg tgaattgggc ttttgcaatg acattaagac aaaaagcaaa
     4561 tccagtcaaa ggaaagaata ctgtcttgtc tacacaattc aatcaaccgc ttcttgaatt
     4621 ctcgggcgct tgctcaggct gtggcgaaac accttatgta aaattattga cacaaatgtt
     4681 cggtgatcga atgatgatcg cgaatgcgac aggctgttct tctatttggg gcggtgcttc
     4741 tcctgcttct ccttatacga ccaatgaatg tggtcaaggg cctgcatggt caaattcttt
     4801 attggaagat aatgctgaat ttggttatgg tatgcttcta gcggcacaga ctcgtcgcga
     4861 ggctttggca agttcaatca aagaagtatt gccaaaagta tcagaagaac tacgagaatt
     4921 gatggaagac tggttggtac acatgtttga gagcgaagga acgcagcaaa gagcagcaaa
     4981 actgcaagct gcgatcgaaa gcgaacgatc aattgtccct gaactggagg atatatacaa
     5041 gagaaaggat ctattcgtaa aaaacagcca atggatgatt ggtggagacg gatgggctta
     5101 cgacattggt tttggcggga tcgatcatgt acttgccagc ggagcagacg tgaatattct
     5161 tgttttggat aacgaagtct attccaatac tggcggtcaa acctcaaaag caacaccagc
     5221 ttcggcaatc gctaaatttt cggctagtgg aaagtatgct tcaaagaaag atctaggaat
     5281 gatggcgatg acatatggaa atgtttatgt ggcgcaaatt gcttcaggtg ccaatcaaat
     5341 gcagacgata aaggcttttg aagaagcaga aaagtatcct ggaccttctt taatcattgc
     5401 ctatacgcct tgtatcacac atggtttgct tggtggaatg aaagaatcgc ttaaagaagc
     5461 aaaagaagct gtttcttctg gctattggtc attatatcga tataaccctg caatggtcga
     5521 aaaaaacaag aacccaatga ctttggacta caaaaaacct gatttctctg ctatgcctga
     5581 tttcatgagg aaacaagttc gcttttcctc gctagagaac gcaaatccaa atcttgcaaa
     5641 agctttattt gaaaagacta tccaagacgc taaaacacgt ttttacaact atgctgcact
     5701 gacgggagaa ttagaaaaaa tcaagaaacg acttgaacca gaggaatcta tcgatgtacc
     5761 cgttaagact agaaaagaaa aaacgacgga ccttgaagca gaagaaagaa gagctgccag
     5821 agcagaacga cgtaaaaaaa tggaacaagc tacaaaagaa aaaaaggaaa aataacagaa
     5881 aaaatataat gaataattct ctgaagttta aaaatattta ttgcagagaa aaataaaacg
     5941 catacttgca attttcctga aaattatgcg tttttctcaa gaaaaatacg agcagatcag
     6001 cctctaaaat taaaacatat gatatactag agaggaaatc tcaaaagaga agagaggctt
     6061 tttatgtcat ttcaaatcgg ggaacttttt aatttggact attggcagca aatgttttcc
     6121 ggtgacttgt tctctctgaa ttttttggtg aacatacttg atattctggt tgtctggtat
     6181 cttgtataca aactgatcca actgctgcga ggcacaaaag cgattcagct tctgaaaggt
     6241 gtagctattt ttattgttat ccgaatcgta gcggaaatca ttggtctgca caccttgtct
     6301 tggctgatga accaagtcat tacgtacggg gtgatagctg ctatcgtgat ttttcagccg
     6361 gaaatccgga gaggactgga acatcttgga cgcagttcgc ttttcaaaca atcaaaaagc
     6421 gaacagcagg aagatgagaa gatgatcttg tcttttgaca aagccatcca atatatgtcc
     6481 aagcgaaaaa tcggtgcgct aattactatc gaacggcata ctggattaga tgagtatatt
     6541 gagacaggaa ttgcattaga tgcagatatc actggagagc tgctgatcaa tatctttatt
     6601 cctaatacac cgctgcatga cggtgcagtt attgtaaaag aaggaaagat cgcagtagca
     6661 agcgcctatc ttcctctatc agaaagtatg ttgattccaa aagaattcgg gactcgacac
     6721 cgggcagctg tcggtataag cgaagtcagc gacgcgatta ccatcgttgt ctctgaagaa
     6781 actggggatg tcagcattac attagataat gaactgatgg ctggcttatc ccagcaagaa
     6841 tatttggcaa ttttgcgtag agaactgatt gctgaagaga aaacaaaaaa caagaaaaac
     6901 gtgattcaat ctttcctaga cggtatgact aagggagggg atcgcaaatg atctcaaaag
     6961 agaaacggac aagcattctt tacagtttta ttgcattgct gttcagtatt gcgctgtact
     7021 tcaatgcaaa tggccagtcc gttccaaata cgctttctgg aaatgaagcg tataatcaaa
     7081 cagttactgg ggtgccaatc aagatttctt atgattcaaa gcgctactat atccatggat
     7141 atgaaaatgc agtgactgtc aaactttcca gtgcaaaccg agttcagttg aatgcagagg
     7201 caaacgaaga tacacggatg ttccgcgtca cagcagatat cactaagctg ggcgaaggaa
     7261 cacatgaggt tccgctaaaa attcaaaatt tgagcagtgc tgtcacagca aagatcgaac
     7321 catcgactat tacagtaacg gttgagaaaa aggtaataaa agatttcgat gtggaaacgt
     7381 tgattccttc aacgttcaca ccatctggat atgaacttga tgatacttcc gttagtccta
     7441 aaaaagtctc tatcactaca ggtgacaaaa cattggcaga aatcaaacga gtagtagctg
     7501 atgtggacgc ttcgatggtc acagatgatg ggattaatag tgaggtccca atacaggcgc
     7561 tgaatgcagc aggggaagta ttaagtatcg tctctgatcc agtgcaagta acagtgagag
     7621 ctgattcgat caagccaagc aagtcggtac gactttatgg gatacagcaa ggaacaccag
     7681 ctgccggggt agaaagctat gatttcagtt tttcaaaact ggaagcagaa gtaagcggct
     7741 ctagcgattt gcttgcatcg atcggtgaca gtattgctgt accgatcaat gtctcaggta
     7801 tcaatcatcg aacaactcgt aaaattgaaa taccagtaga agaggggtta agtatcaagc
     7861 ccgaatcagt tagtgtggag attacacccg tgttgcaaac gtcaagcagt agctctgctt
     7921 cgacgagcag tcggacacaa gaaaatacaa gtacgagtag aagtatttca tctgctgatt
     7981 caggaacgga accatcgaca acaaccattt cttccagttc ggaatctggg acaagcagct
     8041 cgaccacaag tacagaagaa acatcagaaa caagcgaatc aacacaaatg agcagaacaa
     8101 attcctcaga aaattaagtt aaaggagtaa ttttacaact atgggtaaat attttggaac
     8161 agacggcgta cgcggtgaag ccaacaagga acttacgcca gaacttgcat ttaagctggg
     8221 acggtgcgga ggttatgtcc taagccagca tgaagaagaa ggaaaacgcc cacgcgtgtt
     8281 agtagggcgg gatacaagaa tctcaggtca aatgttagaa gaagcgctga tttcaggact
     8341 tttatcggtg ggtatcgaag tgttccagct aggtgtgatc tctacaccag gtgttgctta
     8401 tctgacacgt ttgcaaaaag caagcgcagg ggtcatgatt tctgcttctc ataatccagc
     8461 acaagataat ggaatcaaat ttttcggtgc agatggtttt aaactagtag atgaccaaga
     8521 gctagaaatt gaagcattat tagatgctga agtggatgaa ttgcctcgtc catcagctga
     8581 aggattggga actgtagaag aatttccaga aggcttattg aaatattctc aattccttgt
     8641 acaaacgatc aacggtgacc tttccggttt gaccatttgt gtcgatgcag caaacggagc
     8701 aacttcaaca agtgttaatc gtttgtttgc cgatcttgaa acagattttt ataccatggg
     8761 cacaaatcca gacgggttaa atatcaatga tggcgtaggc tctacccatc cagaacgttt
     8821 agctgagatg gtcgttgaaa aaggagcaga tgctggacta gctttcgatg gcgatggcga
     8881 tcgtattatt gcggtggatg aacttggaaa cattgtcgac ggtgataaaa tcatgtttat
     8941 ctgtgctaga tacttggcag aaaaaaatcg attgaaaaaa gatacgatcg ttacgactgt
     9001 catgagtaac ttaggattcc acaaagcggt ggaaggaatc ggtttgaaag atgtcgttac
     9061 acaagttggt gatcgttacg ttgtagaaga aatgcgcaaa aacgattata actttggcgg
     9121 cgagcagtct gggcacatga ttttcttaga ttttaacaca actggagatg ggatgctttc
     9181 aggaattcag ttactgaata tcatgaaaca gacaggtaag aaattatcag agcttgcagc
     9241 agaggtaaca atctatccgc aaaaattagt gaatatccga gtaagtgata aaaacggtgc
     9301 gatggatgtt ccggcaatca aaaaagtgat tgaagaggca gaagctgaaa tgggatcaga
     9361 aggaagaatc ctagttcgtc cttccggtac agaaccatta cttcgagtga tggcagaagc
     9421 gcctactgat gagaaagtta attactatgt cgataaaatc gcggcagtag tcaaagaaga
     9481 aatcggactg gattaagaga tattttcata aaaaataaaa gaacaaggac cagtgtcaga
     9541 tgtctgacac tggtccttgt tcttttatta ttctactgtc actgatttcg ctaaattacg
     9601 tggtttgtca acgtcgaatc cccgtaatag cgttgcgtaa tatgcaagta attgagtcgg
     9661 tacgactgtg gttagagtag ttagataagg gtggacagtt ggtagcacca gctggtcact
     9721 ttccttttct aatccctctg agacaataac tagtgtttta gcgccacggc tttcgacttc
     9781 tttcaggttt cctcgagtat gcgcaccagt cacttcttca ctgatgattc caatcactgg
     9841 tgttccttct tcgatcaaag caatcgttcc atgtttcaat tcgccagcgg caaaaccttc
     9901 tgcctggata taactgattt ctttcaattt caaagcagct tcaagagaaa cgttgtagtc
     9961 agcgccacgt ccaatgtaaa aggcatttct tgtatcgctg agatatactt ctgccaactc
    10021 ttctaaataa tctttttggg cgatgaccga ttccatcgca ttagcaacta aactcaattc
    10081 atgagtgata tcgaatacaa gagaagtttc aaattctttt ttatcaccga tagctttggc
    10141 taataaagtt agtacagcta tttgtgcagt ataagctttc gttgatgcta ctgcgatttc
    10201 gggtccagca tgcaatagca gtgtaaagga tgcttcccgc gaaagagtag aaccagcaac
    10261 atttgtaatc gtaagtgaag ggtagccgag acggttgatt tttaccagaa cttggcggct
    10321 atctgctgtt tcgccacttt gactcaagaa aataaagaat ggtttttttg aaagaagagg
    10381 tgtgttatag ccaaattcac tggctaaatg aacctcaaca gggatctgtg tcaatttttc
    10441 cagagtctgt ttacctgcta accccgcatg gtaactagtt ccgcaagcaa cgatatagat
    10501 tcgatcgcta tcagacaatg tatcgaccag cttttgatca agagagacac gattgtcttc
    10561 tgttacgtat ttttgagcga tacgacgcat gataataggc tgttcgtcga tttctttcag
    10621 catataaaaa ggataggtac ctttctcgat atctgaagaa tctacttgtg cttcgaatga
    10681 agaacgttct acgatgtcac ctgcaaaatt ttgaataata actttttctg atgtgacggt
    10741 aaccatctct tcatcttcaa tctcaacgaa ttccttcgta tgagcaagca tagccattgc
    10801 atcgctggca atcacattga atccatcgcc gagtccgatt aatagtggac ttttattttt
    10861 agcgacgtaa ataacatcag gtgctgtccg atcaatcaaa gcgaaagcgt aagaaccttt
    10921 gatgacccgc aaagctttta aaaacgcctc ttgcgttgta gtatccttcg cgaaagtttc
    10981 aattagatga gcgataatct cagtatcggt ctcaccaata aagtggtcat ttgctaaata
    11041 agcaatttta agttcttcaa agttttcgat tacgccattg tgaacgagca cgaagcgtcc
    11101 actttgggaa gtatgtggat gggcgttttc ttcagaaggt tgcccgtgag ttgcccagcg
    11161 tgtatgacca atacctatat ttcctgtagt atcaatattt attttgtttt tcaaattttg
    11221 aattctgcct tgtgctttga ccagataatc agttgtgcca tctgagacaa agataccagc
    11281 tgaatcataa ccacggtatt ctaatttttc tagaccgtca atcaatcctg gtgttacatt
    11341 ttttaatcca atcattccaa caattccgca cataaaaaac atcctacttt ctataatgaa
    11401 ttcgtatgaa tgcttccagc tgcaggaaaa tccttttgtt ttatctttgc agatattttt
    11461 tgtggatttt ctgtagagtg cagtccctag ggccatccgc cgaatctttc gataaaccct
    11521 ctcctcgtca tctgtttaca cagacctggc gctatcctaa aaggtataac tatcctcctc
    11581 ttttgttttt tatctggaaa catttaaaat cataaccttt tgcgaatgtt cttgtcaaca
    11641 aaaaaagaat aaaaaataaa ttggtatata tcaaatgctt tgtcaaatgc tctttttatg
    11701 tcaaaaaata atgggttttt atctatcatc tttttcagga gttcggtaca atagaaagta
    11761 atcggataga ggaggagtaa aaatggattc aaaaattgag atcatgacac tgggaatgtt
    11821 aaaaaaacaa ttgtccaaat tcgaagcatc agctggtgta tcagatgata cgaaaatatt
    11881 tttagatact ggctgggata gcatccaaga aatcgcacct gatgccttgg aagttgctga
    11941 agctcgtgag tttacggtag aagatgaatt gacaaaagag tcgttttccg ggtatgcgcg
    12001 ggaagaaaaa gcagagcggt tcgacacttc tgagcagtca gaaactgtga tcgtcataaa
    12061 aaacttgtat tagaaaggag aaagaatgaa taagagacgt tgggcagcag ttctgattgc
    12121 tgcaggatta tttgtggttt cgttagtttc agctagtttg actaagcctc aggaaaacga
    12181 tcaaattgag ggattgaaca gttggttata tggggatgaa gaactagctt caaatgtgtt
    12241 ggaagaggga gacagtacgc aaaaaatcgt aaaattatca gtagatggaa cgattgctgc
    12301 gggagggtct tcaggtttat ttgcttctga aggttacaat cataaaaatt ttatggaaca
    12361 actgaaggcg attgaagaag atccaacagt caaaggtatc tttttagaag taaattctcc
    12421 tggtggcggt gtctacgaaa gtgcggaaat cgctaagaaa ttggatacga ttcgtaaaga
    12481 acatgatatc ccaatgtatg tcagcatgaa aaacatggca gctagcggtg gctactatat
    12541 ttctgctcaa gcagataaaa tctttgcaac agaagaaaca gtgaccggct caattggtgt
    12601 gatcatgtca ggattgaatt attcaggact tctagaaaaa ctgggtgtgg aagatacaac
    12661 agttaaaagc ggagcgttga aagatatggg ttcaggaacg cgaccagaga caaaagaaga
    12721 agaagcggtg cttcaagcat atatcgacag cgcatatagt cgttttgtag caatcgtcag
    12781 cgagggacgg aataaatctg aagaagaagt caaaaaaatt gctgatggaa ggatttatga
    12841 tggtgtccaa gcaaaagaag taggtttagt cgatgaattg ggatttcctg aagatgcttt
    12901 aaaagctatg cgaaaggaac aacagcttga agatgcggaa ctagtagaat attcttccac
    12961 gccatctggg tttgctagta catggttagg aagtaaacta gcagaattcc agggattgaa
    13021 gccttctgag actagtcaga tcttatccgt tcttgagaat ctagggacag cggaatcacc
    13081 aaaagcaatg tattattacg gaggtgagta agcatgtcag agctttcaaa tgaaaataag
    13141 gaacaagaaa aagataagca acagcagcct gctgtaggac ctgactccaa cttttcccaa
    13201 aaggtactga gctcttttga aagagagcct ttgacgcccg aagaaatcaa aaagaaacaa
    13261 cgtaaatgga aaaagtatac ggacacaaga aatgaaccgc caaaagcaaa tgattttcct
    13321 aagtatttct atgcaggttt ttttatccga atgtttgctt tcttggtcga tcttttgtgt
    13381 atcggagcta ttacgcgttt aacacttggt attgccaaaa atctaggttg gcttaatttt
    13441 tcagattctt atctgagtat ctacggattc acagccttac ttatttatct agcgtatttt
    13501 attttattga cgaaattcaa tcatggtcaa acgattggga aaatgatctt tggtattcga
    13561 gtcatcagct ttgatgaaga agaacttagc tggtcaaccg ttttagttag agaaggtgct
    13621 tgccggttca ttctaaaatt tcctttgctg atgttagggt atcttccagc aatctttaat
    13681 cagaaaaagc aacatctagg cgattttttc actaatacaa gtgttgttac gatcaatctg
    13741 atcaaagcat tcaaccagga agtcaacagc tgatgagcaa taaaaagcag taacagttga
    13801 aagcaccttc taaaagtcag atctaaaatc tagcttttag agggtgcttc agttattttt
    13861 aattgtcatg ctttttttgt tttgctacta ttgaacatga gctgaacatg agcaagagga
    13921 ggacactgcg tgaagccgat tgattttcca gaaaaatatg agaatttaat gcgagtagca
    13981 caacaagcac tagcaaacca gcaatatcag caggcaaaag aattatttca acgagcttac
    14041 gagttgaaag aaagctttga ggctaatagc ctgcttgttt tttgtcttta cgagttggac
    14101 gagaaaaaag aagcattgaa gcaagcactc ttgcatgaaa aacagtatct tcaaaatgag
    14161 gagtttgctg aattttattt tgacttgtta atcagtacac aagatttttt atatgctaga
    14221 aaattgatag caagtacaga tttttatgaa gctttcgagc agcgggtcat tgaaaaaatc
    14281 caatttgcag aagaattatc cggacaaatg gagaggcaga agatcaaggc actccacaat
    14341 aaatcggagg aacttccttc gttggaacct gcacgtcagc ttgcgtctat tgatgagatc
    14401 gaacagcttc cttatcatga gtttatccaa acagcgagta aacttattgt atcgccagag
    14461 gtccatattt tagcaagggc aaaattactg gagacactta ggcaattgaa cgaatgccat
    14521 cctgttttct atttgacgat tgaagaaaag ttagtcaaag tcataccaaa agatctgcca
    14581 aagccgcagc agcaggcaag ttaccagcaa ttatgtgtat tttcagatcg ttacggtaat
    14641 gaagatgcgc tgcttagttc cgtgttaaat gaagaattca cgttgcaatc tgcaattgtt
    14701 tatcctgtct atgatacata catcgaagac ccgaagcgct ggtttcaact gactgtagag
    14761 gcgtgtacag gaaaaacgat gtatgaaggt acagaagatg aaaaacaaga ttttttaaaa
    14821 aaaagagaaa aaattttgca gcaaatgatc ttcttccatt agattttatc agtgaaaaag
    14881 ataaaaatgt tatgaaaaac aaggattcta tccattcaat atgtttacaa tttgaaaggg
    14941 ttagtgtact atttaaaggt atgcatgaaa cgtttttgat gtataaaatg aatttctaaa
    15001 ctcggaggga attaaatgac tgcaacattt gaaaaaaaag gcacgaatga tggtgtttta
    15061 aaatttacga ttgcgcaaga cgaaatccaa aaaggattaa caactgcgtt taataaagtg
    15121 aaaaaaagct tgaacgtacc tggattccgt aaagggaaag tttctcgtca agtattcaac
    15181 cgtatgtacg gtgaagaagc tttatatgaa gatgcattga atgctgtatt accagaagct
    15241 tatgaagctg ctgtcaaaga agctggcatc gatcctgttt ctcaaccaaa aatcgatgtt
    15301 gaaagcatgg aaaaaggtca agactgggta atcactgctg aagtaacagt gaaaccagaa
    15361 gtaaaattag gacaatataa agatctagtt gttgaaaaac aagaccgtga agtaacagat
    15421 gctgacgtgg atgcacgtat caaacgtgaa caagaagcac aagctgaatt agttatcaaa
    15481 gaagatgctg cagctgaaaa tggcgataca gtagtaatcg acttcgaagg ctttgtagat
    15541 ggcgaagcgt ttgatggcgg aaaaggcgaa aattactcac ttgaattagg ttcaggctca
    15601 ttcattccag gttttgaaga tcaattagtt ggtcataaat caggtgaaag tctagatgta
    15661 aatgtaactt tcccagaaga ttatcaagca gaagatcttg ctggaaaaga agcagtcttc
    15721 aaagtgacaa tccatgaagt aaaagcaaaa gaagtgcctg aattagatga cgaattcgca
    15781 aaagatgttg atgacgaagt atcaacatta gacgaattga aagaaaaata tcgcaaagaa
    15841 ttagtagaaa caaaagaaaa agcagcggac gatgcaaaag atgaagcagc gatccgtatg
    15901 gctgttgaaa acgcagaaat cgttgaattg ccacacgtga tggttcatga tgaagtacat
    15961 cgttcaatgg acgaattctt gaacaacatg caacgtcaag gtatctctcc tgaaatgtac
    16021 tatcaattaa ctggttctac agaagaagac ttgcataaac aattcgaagg tgaagcagaa
    16081 atgcgcacaa gaacaaatct tgtgatcgaa gcaattgcag ctgctgaaaa tcttcaagca
    16141 acagaagaag aaatcaatgc agaaatccaa gaattatcag aaacatacaa catgccaatc
    16201 gaacaaatca aacgcgtgtt gacagaagat atgctggaac atgatgtgac aatgaaaaaa
    16261 gcaatcgaat tgatcacagg tacagcgaca gaagcgaaag aatagaaatc aagtaataaa
    16321 tcatttctga agaagctact tctagaaaat tgtttattcg aaataaggga ctgtgacacg
    16381 attttgtcac agttccttat tttttgttaa gagtaaaaaa tgcaaaggta taggtcagtt
    16441 gatcctttgt taaacacttt ttctttttac taaactatgt tatgatagta aaagactgta
    16501 aagtctttga tagtttcttg catacttttt gcaagttaag gggtgaagac catgtatgac
    16561 aatccaagta gcaatgagac cgtacgctgt tctttttgcg ggaaaaccca agaagaagtg
    16621 aaaaagatcg tagctggtcc aggggtctac atctgtaatg aatgtatcga cctatgtaaa
    16681 gaaatcattg atgaagaatt ttatgaagaa gctgtacgcg aattcactga agtccctaaa
    16741 ccactagaaa ttcttgaagt attgaataac tacgtgattg gacaagatcg cgcaaaacgc
    16801 tcgttagctg ttgccgtata caatcactat aaacgtgtca atcagcaagc acaagaaaac
    16861 acagatgatg ttgaactgca aaaaagcaat atctgcttga tcggacctac agggtctggt
    16921 aaaacatttt tagcacaaac attagcaaaa acattgaatg taccttttgc aatcgcagat
    16981 gcgacaagtt tgacagaagc tggttatgtg ggggaagatg tagaaaacat tttgcttaaa
    17041 ttgcttcaat ctgctgatta caatgtggaa cgtgcagaaa aagggatcat ctatattgat
    17101 gagatcgata aaattgcgcg taaaagcgaa aatgtatcta ttactcgtga cgtttccggc
    17161 gaaggtgtcc aacaagcctt gctgaaaatt ctagaaggaa ctgttgcaag cgtgccacct
    17221 cagggaggac gtaaacatcc acatcaagaa tttattcaga ttgatacgac gaacatattg
    17281 ttcatcgtag gtggtgcatt tgatggcatc gaaacgattg tgaagaatcg tatgggcgaa
    17341 aagacgattg gatttggtac acaaaaccaa aaactttctg aagacgaaag tgtcatgcag
    17401 catatcattc ctgaagattt actaaaattc ggattgatcc ctgagtttat cggacgatta
    17461 cctgttatgg cagctttaga gaaattgaca acagatgatc tggtacggat cttgactgag
    17521 ccgaaaaatg cgctagtcaa acaatatcaa aaacttcttt ctctagataa taccgaactt
    17581 gaattcgaac ctgaagcgtt gagagccatt gcgaaaaaag caatcgaacg caataccggt
    17641 gctcgaggac ttcgttccat cattgaagaa atcatgatgg atgtgatgtt tgatatccct
    17701 tcaaatgaaa acatcgaaaa agtgattatc acaaaagaag cagctgaatt gtctggtgag
    17761 ccaacagtaa tctataaaga ttcagagaaa aaagcgggtt gatgaaaaga ggctgccgta
    17821 aaagtcattt tggcttttgc gacagccgct tgctttattc atcaccttgt taaggagaga
    17881 gaccatgaaa gtccatcaag ctgaaattat cataagtgca gtggcaccaa aacaatatcc
    17941 agatacacaa ttgccagaaa tagctctggc aggacgatca aatgtaggga aatcttcttt
    18001 tatcaacaca ttgattgatc gaaagaattt agcccgtact tccggtaagc caggaaagac
    18061 ccagacattg aatttttatt tgatcgagaa cgcattacat tttgtcgatg ttccgggata
    18121 tgggtatgca aaagtatcca aaactgagcg agcaaaatgg ggaaagatga tcgagacata
    18181 tttaacgact agagaccagc tacgtgcggt cgtttcttta gtagatttgc gtcataagcc
    18241 ttctcaagaa gatatccaga tgtatcaatt tttgaagtat tatgatatcc ctgtaattgt
    18301 tgttgccact aaggcagata agatccctcg tggtaaatgg aacaagcatg agagcgaaat
    18361 caaaaaagcg ttggattttg atcgcaatga cacgtttatt ctcttttctt ccgttacaaa
    18421 acagggaaaa gaagaagcgt gggcagcaat cgaacaagca attgaatcat aaaaagaata
    18481 aaaaagactt accagtcaaa ctctttcata aaagtttggc tggtaagtct ttttttgagc
    18541 ttaacttact tttgtttctt gtcttttttc ttttcatttt gtggctgtgg ttttagaaat
    18601 cgatcgatct cttcttttgg tgcaactgtt ttttccggat cgacagcaaa tgattcgaaa
    18661 agtttatcta aattatctgt acgttcaaat ttcaacccca ttgtaaacac ctctttgttt
    18721 ctttttactt agtctatcat gtaatcgtcc aaattggttt ctaagacata aagttttagt
    18781 ttttctttat cgttggttat aattaaatta taaaaagatt ttaaagaggt gagaaaatga
    18841 ttggcgaaga taaattattg atgactatcc agctcacaga cttaaatgaa gaagaaaaaa
    18901 aacaggaaat aagcgattgg gctattttga cacgtctgtt gatccagcca acatttatcc
    18961 gctctttcac acaacaagcg gatccgtatg atcttcgaaa gcttcaagaa aaaataatgc
    19021 tagcatcggt aagagacgaa agttggttag ttgtaggaaa tgacgagaac gaatgtagtt
    19081 ttcgcttgga agataatcaa ttattgataa aaaatgtatt gtctatctct gttttcaaag
    19141 aaaagcaatt tttgattcga gacttcattc agaaaaaaat gattgctcat ggggtgttcg
    19201 cctatatgcg agcttatagc gagtttatct atcacaatac aaaacagatc tctcaacggc
    19261 tattgtttga gaaaaaggat gaaatcgaac atttacctaa aatgaaacaa caaaacgggg
    19321 aagtagtagt cgattgcaac caatttccag gatacgatct tttttaccaa ggtttatgtt
    19381 tcacttcgtg ttgggaaatg tattatagtc gctattatca tcaaatcatc cctaaaccga
    19441 tttttctcga catccaacaa gtagaaaaag tgaaggaatt agaaaacgaa gtcatatgta
    19501 tccagcttta tcgagacccc tttaactggc aaaagccaaa caatcaaatg tttcaatcct
    19561 attttaggga ccagcttggg tttgatcatc ttgcttggga caatggtgtc ggtttattga
    19621 aaccgccatt cgttgaatac atttacactg accatacgat ccaatctgtc cagtatcaga
    19681 atgcacaaat gcaacctgtt cctaaaaaga atgcttcatt ttttgtaaca aaaagttatg
    19741 atattcaaca aggagattat aaagaaaggc gtgttcgcgg aacactaaat gctcaggcgt
    19801 attttccttg ggttgacgag aatcgctcgc gcatgatgtg ctataaagtt atcgatccga
    19861 ctgttgcttt ggataatgga atcgaagctt attgttatta tattagggaa tatctagaag
    19921 tagaggtaac agatgaaaaa tatcaagagt atcttttatc cttaagaatc tatgttcctt
    19981 cagaatccct gtcagaatta ccgttaaagg aaatcaaaca tagactctct gatgttacct
    20041 tcaaacgaat aaagaaaaag cggcgaagta tccaattcga tgtcaagaaa ggtgagcagc
    20101 atctgcgtgt gcaattttta gattaccgag aattagaaca attgattact ttacaaaaaa
    20161 tatgaggtga agaaaatgga taaacaactc tattatcgaa taatggttcc ggcatcatta
    20221 ttatccgtct gtctcttttt tgttcctaat tttcctattc gatcagtcat cggttttttg
    20281 gtcatgtttg gcgggatggg actttattat tatcttcttc ataaggaaag aaaacctgct
    20341 tctgtttata tgaaaaaaac aagaagcgaa aa
//
LOCUS       DAZUKA010000045        19875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000045 DAZUKA010000000
VERSION     DAZUKA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.045"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..256
                     /locus_tag="ACG8PN_001266"
     CDS             <1..256
                     /locus_tag="ACG8PN_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861430.1"
                     /translation="QNIEGSYIEVKTLFVNEDKTKALDIYKKLASKKTNSFGLILSEY
                     KIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(900..1853)
                     /locus_tag="ACG8PN_001267"
     CDS             complement(900..1853)
                     /locus_tag="ACG8PN_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4861431.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1876..2631)
                     /locus_tag="ACG8PN_001268"
     CDS             complement(1876..2631)
                     /locus_tag="ACG8PN_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861432.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2628..3587)
                     /locus_tag="ACG8PN_001269"
     CDS             complement(2628..3587)
                     /locus_tag="ACG8PN_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4861433.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3580..4530)
                     /locus_tag="ACG8PN_001270"
     CDS             complement(3580..4530)
                     /locus_tag="ACG8PN_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4861434.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4680..5132
                     /locus_tag="ACG8PN_001271"
     CDS             4680..5132
                     /locus_tag="ACG8PN_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4861435.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5221..7488)
                     /gene="gshAB"
                     /locus_tag="ACG8PN_001272"
     CDS             complement(5221..7488)
                     /gene="gshAB"
                     /locus_tag="ACG8PN_001272"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4861436.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7675..8673)
                     /gene="ftsY"
                     /locus_tag="ACG8PN_001273"
     CDS             complement(7675..8673)
                     /gene="ftsY"
                     /locus_tag="ACG8PN_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4861437.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8684..9493)
                     /locus_tag="ACG8PN_001274"
     CDS             complement(8684..9493)
                     /locus_tag="ACG8PN_001274"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4861438.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9502..13083)
                     /gene="smc"
                     /locus_tag="ACG8PN_001275"
     CDS             complement(9502..13083)
                     /gene="smc"
                     /locus_tag="ACG8PN_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4861439.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13103..13789)
                     /gene="rnc"
                     /locus_tag="ACG8PN_001276"
     CDS             complement(13103..13789)
                     /gene="rnc"
                     /locus_tag="ACG8PN_001276"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4861440.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14137..15918)
                     /locus_tag="ACG8PN_001277"
     CDS             complement(14137..15918)
                     /locus_tag="ACG8PN_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4861441.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15944..16858)
                     /locus_tag="ACG8PN_001278"
     CDS             complement(15944..16858)
                     /locus_tag="ACG8PN_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861442.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16885..17847)
                     /gene="opp4B"
                     /locus_tag="ACG8PN_001279"
     CDS             complement(16885..17847)
                     /gene="opp4B"
                     /locus_tag="ACG8PN_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4861443.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17847..18791)
                     /locus_tag="ACG8PN_001280"
     CDS             complement(17847..18791)
                     /locus_tag="ACG8PN_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861444.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18792..19796)
                     /locus_tag="ACG8PN_001281"
     CDS             complement(18792..19796)
                     /locus_tag="ACG8PN_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861445.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5515 a         4269 c         3537 g         6554 t
ORIGIN      
        1 acaaaatatc gagggttctt atattgaagt aaaaacactc tttgtcaatg aagataaaac
       61 caaagcattg gatatttata aaaaattagc ttcaaaaaaa acgaatagtt ttggattgat
      121 attaagcgag tataaaatca aagcagaaga gagttatttc tatcaattac taaaacggtg
      181 gagtaagctt cccgccgatt tttatcgaaa aatgcagatc atcaactatc aaccgcttgc
      241 tgagacacat gcctaaatag aggttgtgaa atcagtgctt tgagctaata ccgaaggtca
      301 gctgattgaa cattgtttag aagagattgt gacaactgcg atttgctttg agtaataaga
      361 acgctggaca gaaaatagct tctcatattt tctagtctag tgggcttatt gccgagaagc
      421 agcagatgga acatcgttta gaagagattg tgacaactgc gatttgtttt gagtaataag
      481 aacgctggac agaaaatagc ttctcatatt ttctagtcta gtggacttat tgccgagaag
      541 cagcagatgg aacatcgttt aaagaagaga tcggtacaaa ataaaaatat ctaaatttaa
      601 gttttatcta agtttcgctt tgtacctttg attcaaatca aaggaggcga tcttgatgct
      661 gaaaaaatgg atcacactat tttttgccac tccccattct agaatcaacc cgtatgatgt
      721 agataatgaa ttcacgttga aaaaagattt ctcaaataaa taaaaaccat ccgtaaaatc
      781 atcaatacga ttttacggat ggtttttgat aacttctttg aggataggct cttgctattt
      841 aagaatgtaa gaaacattat tgttacatag tatgagagtt taatcctctg ttatttatgc
      901 tatttaaatg cttgattcac atcttcgatc atcagtttca ttgattctaa accgcccccg
      961 cttaaatacc agacatctgg ttcaagggag atgatctgct gatttttacc agcattcgtt
     1021 tgtgctacca gttcattggc agaaatatca tctttgctgt cgtctccacc aatagctttt
     1081 gttctgtcta ctacaaataa gatatccgga tttttttcca gcacatattc ataagaaaca
     1141 ctttgaccgt gagtggatgc ttcgatttga tcatctgctt gttcaaagcc aaataaatca
     1201 tggatgaaac cgaatctaga accagatccg taggctgata attgcccttc attcacaagt
     1261 gtgactagtg cttttttgtc agtagcttct gctttctttt tcgtctgatc gattgcacta
     1321 gataattcct ctaattgagt tttagcttct tcttgcttac caaagatctc ccctaaagca
     1381 gtgacatttt gctggaaaga ttcccatgga tttttcgcat ctaaagaaag gaaaatggtc
     1441 ggagcaattt gacttagatc tttttgaaaa tctctttgtc ggccagaaat gatgattaga
     1501 tctggctgaa gctgattaat tttttctaag tctggttctt ttattccacc tgctgattct
     1561 acttttttat aggatgaaag atactcagga aggctgcttg tagctgctcc aactacctta
     1621 tctccaacac caagagcatc catcgtatct aaagaaccat tgtcgaatac gacgactttt
     1681 tctggattat ttggcacctc gatttggtct ccattactat ctgttatcgt caatgtagtt
     1741 tcagtttgtg tgctgctttt ttttgtgtct gcctgactgg tgttgttgct atttccacat
     1801 gctgctaaac caaatgatgc tagaatcaag atacctacta ctaatttttt catctcttct
     1861 ctcctaatgt tagttttaga aataaagaca gaatctgcgg ccttcaatct cacaaatccg
     1921 tatgtccatt tcgtataatt catccaatac atcttttttt atcatttctt gggtcgttcc
     1981 ttgcgtaaat aactgtccat ctttcattgc aacaatttcg tctgcataac ttgcagcaaa
     2041 gttgatatca tgaagaacaa taacaattgt tttcccgatt tcttgtacta aattttttaa
     2101 aatctgcatg atttgaaccg caaaattcat atctagattg ttcaaaggtt catccagtaa
     2161 aatataatcc gtgtcttgcg ctagcaccat tgcaatatac gcacgttgga gttgaccgcc
     2221 tgaaagagtg tcaatcgatt gatccgctat ttcttctaac ccaaggtgtt ccagtgattc
     2281 ctgaacgatt tcctcgtctt ttgtcgtcaa ttttcctttc gtataaggaa atcgtccaaa
     2341 ttctactaat tctttcacgg tgatttggac atttatttca ttcgattgtc gtaggatcga
     2401 taattttttg gctaattcat tggatttcca agcctttact tcgttatggt ctaaatagat
     2461 ttcacctgta tcttttggta tcaaacgact gatcatcgac aacaccgttg actttccagc
     2521 accatttgga ccgataaaag cagtcaactt cttttcagga atcgttaact gtacatcttt
     2581 aaccactgat tttccatggt acagtttatt gaccttttgt aaatcaatca taggatactc
     2641 ttcctttcgc gaatcaattt tgtgacaaaa taaattcctc cgacaaattc aacgacgaca
     2701 ctcactgttg tattccagcc gaaaatctgc tctactgcga gctgtcctcc tgtcagaaac
     2761 agcatagcta ccagaaagct tccgataaat agctgaccat gacgataagt agaaaagaca
     2821 cgataactga tatttgcgac aataaaccct aaaaaagtaa ctggacccac aagagccgtt
     2881 gatgtccctg tcaaaagact gacacaacat aaaatcaaaa attcaaaacg atctgtgtct
     2941 atcccgaggt ttactgcttg ttcatttccg agatggaaaa cgtctagcac atgtcgatat
     3001 cgaaataaca ggtaccagca gccgataatg atcaaagaag caggcaaaag atactgactc
     3061 tggatattgc caaagcttgc aaataatctg ccttgaagca aatcatattc attcggatcc
     3121 atgatgactt gtaaaaaggt actgacacta ccaaaaagcg tacctaaaat catccctgac
     3181 atcagtaata aaaaaagatt cgctctcatt tttttcaaaa gaaagaaaaa taacaataga
     3241 ctcagcccga ccatcagccc aacgttcaat aagaaaaaaa ctgtacctgt ctgctgaagc
     3301 atcgtcactc ctccgataaa gaagaaaagg accgtttgga caaggacata taacgactca
     3361 agtcccaata ctgaaggagt caaaaactgg ttatgcgtaa ttgtctgaaa gctgattgtg
     3421 gcactgctga cagctagcga gacaaggata aatgcaaaaa ttttcttact cctcaatgca
     3481 catgcaaaat gccagtttcc atatgtatgg atcgtcaagt acattcctgt aacaaataaa
     3541 acaagaacaa gcataccaat aaaaatagat ggcttttttt taagcatatc gtcgtcccct
     3601 tatcagaaga taaaggaaga tagccccacc gatgattcca acgatcacac tgactggtat
     3661 ttcataagga aaaatcaaca cgcgagcgac aagatcgcaa aatactaaga acaaactacc
     3721 tgctaaagca gttacgccaa ttgttttgtg cacttgatct ccatatattc tagagacaag
     3781 attcggaatg atcactccga taaaaggaat gcttcctacc atgatcaaca tgactgcact
     3841 agccaatgat actaagccaa tgcctaaaaa ctgcatcttc tggtaatcta ttcccagatt
     3901 tactgcaaac gattcgccca gaccaatgat cgtaaatcgg taagcaaata gccaaaccaa
     3961 gagcaacaat ggaacaacag catacaataa ctcataattt cctcgtatca cagtcgcaaa
     4021 attcccttga agccatgaag tcatattttg taccaactgg aattgataag caaaaaaagt
     4081 agcaactgaa ccaatgatat tccctaacat gactccaatc aacggaagca tcaattgatt
     4141 tttctggagg aacaatcgcg taagggataa aaaaagcaat gttcctgcaa aagcaaagca
     4201 aaatgctgtc aaggatcgga caatcaagga tgcaccaggt aggaaaatca tagccactaa
     4261 aatacctaaa cgcgcactat ccatcgtgcc cgcagtcgtt ggtgaaacaa atttattttg
     4321 ggtcaaatgt tgcatgatca acccacaaat actactggtc gctcccacaa ggatcaaaca
     4381 aatcgttcgt ggcaaacgag tcactagcaa gatttgtaag ctttgcccat ctaactggag
     4441 cagttctgac aattgtacat tcgatactcc gatgaacaat gaaatactac ttaaaataac
     4501 taataatgct actagtcgtc cgtagcgcat ccaatccacc tttctaattg agaatgattt
     4561 tcattcttat tttaagtgaa tcgttgaaca aaagcaaccg ctttttcata tgttttaggt
     4621 attattggga atatgctata atcgtaagaa agacaaagag aagaaggagg gtatttttta
     4681 tggcacaaga tgatgtaaaa agccatttgg ataagggact ttatggtaca cctttagtga
     4741 accctgaaga acagcataaa tatttaggga cattcaggga acgctgctat cttagtatga
     4801 ctgttgctga aatgatccaa ccaaaaaaca aagaaaactt tttaaaagaa ctcaaaaaac
     4861 atccagatgc ttccatactc ttgaatgggg ctctacccct ttctgtccaa aaccaatata
     4921 tccagctagc aacgaaaaac aatagccgtt tcactgtagt caacgattac gtcactgcaa
     4981 atcctgagga attcggattg ctcttaacag caaaagaagc agtaaatgaa cctgtgattg
     5041 atatcgaaaa aaaataccca gaagaaaact cttcgccagc tgaaaaagaa aaaccaaaaa
     5101 agaaaaaaag cttctgggat cgtttatttt aaaacgaaaa aacacataaa tttttacatg
     5161 caaaaaaagc ttttgatcag attgacatca aataggacaa tctgtcaaaa gctttttctt
     5221 ttaaaatact tcaggatata acaatttcaa tacattcatc gtcaatcttc tgccttttcc
     5281 tgcaaaagga taaacatgca tatgcatggc tggattgaaa tttgcttcga tgatcccata
     5341 tgctttttta tcagttgtag gatcgatttc tttatctggt ataatcaaat cgataccact
     5401 aatcttagct cctagtgctt ctaccgcaga aacggcgatt tttttgtaag cttcagaaaa
     5461 ttcatcagtc atatcgatcg aatcaccacc tgtactgata tttgagttct cccttagata
     5521 aacaatttga ttggcttggg gaactgattc gatcgtcaac ccttgttctt tcaacatcaa
     5581 ttgttctaat tcacctaatt ggatcaattc taaaggtgca cgatgattcg ttccacgcaa
     5641 agggtcacta tttttttctt caacaagttc tttcactgtt cggatactgt caccaataac
     5701 attagcaggg actcgcaaca tgatggcttg gacttctccg tcgatcacaa agaaacgata
     5761 ttccgttccc ggcataaatt cttctactaa aaccgaactg tcttcacgaa aagcaatggc
     5821 taaacctgct tgataatctt ccagactagc tccttccttg aaaatcgtga tacctaaccc
     5881 gtaattggtt gatttaggtt tgatcacaaa tgcctgctcg gcaaatctcg gataagcttt
     5941 taccgcttct tccatggatg aaaattctgc gcctcctggt acccggaacc cagcttcttt
     6001 taagactttt ttcgttactg ttttgttttc catgataagt gggacgatat agctgtcctt
     6061 acttgtcatg tttgcatttt tcacatattc tacatggtct tggaattgca gacgcaaaaa
     6121 ttgatccgac tcatctaata ctttgacttg gacacctttt tggatggcat cgaacataaa
     6181 tatctgagta gaaagttcca tttcgcgaaa accggccaac tgatagggac gttcatggct
     6241 ttctttataa tattgatgtc ccaatgccac agcaacttct tgctgactcg atagctgtgc
     6301 ttcttgatag agtttcgctg ctaacgtttg ttcaggatga tccatttgag tcaatgcttc
     6361 ctctactaaa taaagagaat ccaaccaatc caattcaacc agcatttgct tcattccttc
     6421 aagaatttct ctaccttctt gatgaaattc tgtttgatct gatggttgtt ctagtgctac
     6481 tttgttattt cgagtcgtac cttctgctac ccattggtca cagtcttctt tttcttctgt
     6541 ccataacatg tacatcagga ataattgtaa gaaccgaact tgctctggat tgatacccaa
     6601 tcttgcataa ggattcaaat cgatattccg caattcaata taacgaacac ctgttgttgc
     6661 taaatctgcg actttctttc cgcctcggaa acgaagcggt gtataaaatt ctttctcctc
     6721 cgaaagtttc ccttcttcga tcattcgctc gatatctgct aaatactgtt tcatagaagc
     6781 ataggaaact ttcacatttg gatgattcgt atagcccagc gcactgttgc gaatactgcg
     6841 aacaggttct tgcggatgtg attcatctaa aaagtagttc ttctcactca ttggtgaagc
     6901 accgaataaa taagtgatca tccagcgata gcgcataaag tttctcgctg ttttcaaata
     6961 aagttctgtt ttgaattcgg aaaaatcttg aaattcttcc tgatggctga acaatgttct
     7021 tagcaactca tcgccaaatt caaaattgaa atgaataccg cttaccatct gttttcgctt
     7081 cccgtatttt tttgctaaat aacgtcgata caagacatct tcgaaatttt ttaattttgc
     7141 aataatgatc tcttcgtctt tttccggtaa ggcaggtggc atacttaatg gccagatcat
     7201 ctcttctttc ggtatagaac gagcagtcac atcataaaca gcagcgagat actgaaataa
     7261 ttcgggaata gaatcggtaa caggggtgat catctctgtt tgtgtttcgc taaaatctgt
     7321 ttggatatag gggtgatagg atcgatcgcc aaagattgct ggatgatcgg tttttgctag
     7381 atttcctgca agatcaaccc gctgtccttc ccgttctata ccaaatcgag cttggtcgat
     7441 aaaagggcgc gcattgacat gcaataataa ttgcttaaaa ttcatcatta agtgacctcc
     7501 aattcatatg aatctaatta taatcaaaaa gtctaagaaa aagaacgaaa ttgtttgaga
     7561 accgcttaca aataagtata aaaatgaagg catcaaaaac acagctatgt taaaaacaat
     7621 cattcaaaca aggagctgca acttttgtca cagctcctgc tttttagaca tgggttattc
     7681 ttcttttagt aatcctttga acaatccaac gacaaaatca tttggatcaa acggctccaa
     7741 gtcatcgatt ccttcaccaa gaccgaccaa tttcactggt aaatgcaatt cattacggat
     7801 agcaagtacg ataccgccct ttgctgtacc atctaatttt gtcaaaacaa gaccggtcac
     7861 atctgttgtt tctttgaatt gttttgcttg aaccatcgcg ttttgaccag tggttgcatc
     7921 taatacaaga agtgtttctt ggggtgcttc tggtaattcc cgctggatta ctcgttttat
     7981 tttttctaat tcgttcatca agttcacttt attttgcagt cggcctgcag tatcaactag
     8041 taaaacatct gcctgctctg tctttgctct ttccaaagca tcgaatacga cagctgctgg
     8101 atcgcctcct gcattgccac gaacaacttc aacaccagcg cgttcgcccc agacaactaa
     8161 ctggtcgatt gctccagcgc ggaaggtatc tgctgctgcc atcaatactt ttttaccttc
     8221 gtttttgaac tgatgggcaa gtttaccaat acttgttgtt ttccctacac cattaacacc
     8281 gacaaacaaa ataactgtta gtccgtcttc ttggatattt agttcattta cttcattgat
     8341 tccttccgct tcatagagat ccacaagttt ctcgatgatc gcattttgga cttctgccga
     8401 ttttttcgca tttctgagtt tcacttcttg acgtaatgct tcggtgattc gtatcgctgt
     8461 ttcaaaacct acatctgcac cgataagtgt ttcttctaat tcttcaaaaa agtcttcatc
     8521 gactgttcga aaattggcaa acaactcatt catccgttca ccaaatgttt tacgcgtctt
     8581 ttctaaaccc ttgtcgtact tctcctgtac ttcaacttct tctgttttct cttcgccagt
     8641 aaaagcacgc ttgattttat caaaaaatcc catttgactc cccctactct aatacaaatt
     8701 tttggatcac tttggctacg ccattttctt cattgctggc tgtcacgaaa tctgctgctt
     8761 ctttgacgaa aggcacagca ttctccatcg ctacgcctag tccagcatat tggatcatcg
     8821 gtaaatcatt ttcctcatca ccgatcgcca tgatttcttc ttgtttcaaa ccaagatctt
     8881 ttgccaataa agaaatacca taagcttttg taacaccttt tggcataaat tccaaaagca
     8941 tacttcttgt tttgatgatc tcatacattt catggaattc tgctggtatt tctttgattt
     9001 taggatctaa ttcttcttgg tcataagcga ttactgcctt attaagcaca aattcatctg
     9061 taatatcgct tatatccgct ggttggaatt gcagcagctt attcaacaca ttgtacaagg
     9121 attgcttcct tggggcacta ggcagctgta aaaccacatt gtcagacaaa acatctagtg
     9181 gcagatccaa ttgctgagcc agtttgtata agcgttggat atctgatact tccatcactt
     9241 tcttttccat gatttcacca gtatcatttt tttgtaccaa accgccgttg aaagtgatgc
     9301 tgtagtcacc ttcatctacc agtcctaatt cttgcagata atgcgccatc gcagctaagg
     9361 ggcgtcctgt acatagaacg atcttgaccc cttgctgttt tgcttgtgct aatgcttgtt
     9421 tattctcatc tgaaatcagc ttttgttcat ttaataatgt accgtccaaa tcgattgcta
     9481 ctaatttgat cattttcgtt cctacacttt cgtttcgttg attttcgcct cttcattgat
     9541 atcttccatc cgtacagaaa cgatttttga tacaccggat tcctgcatcg tcactccgta
     9601 cagtacatct gctgcgacca tcgttccttt gcggtgtgtg actacgataa attgtgtgtc
     9661 atcttggaaa tcgctcaagt aacgaccaaa acgtttcacg ttggcttcat ctaacgctgc
     9721 ttctacctca tctaagatac aaaacggaac aggacagaca cggataatgg aaaagagcaa
     9781 cgcgatggct gtaagtgctc gttctcctcc agaaagcaaa cttagacttt gcagtttttt
     9841 gccaggaggt tgtacttcaa tctcaatacc tgttttcagc aaatcagttg gttccgtcag
     9901 tactaattca gctttaccgc cgccaaacat atttggaaag acaattttga attcttgacg
     9961 gatagcttca aatacttctt tgaatcttgt ccgcacttca tcatccatct catccatcgt
    10021 ttcaaacaat tggtttttcg ctgctaacag atcatctcgc tgtgttgcta agaatgtatg
    10081 tctttcactg acttgttcgt attgttcgat tgaatttaag tttactggtc ccagtttttc
    10141 aatctgttct tttaaggatg aaacctttgt tctactgtct tcgatatcgg ttgtttcttg
    10201 atagtcttgg gaagctttct caaatgtcag ctgatattct gactgcagat aggtcaaatg
    10261 attatctaaa atcatttcag ctcggtcttt ttggacttct aatttggatt gttccgacag
    10321 tcgtgctttt tgctcacgat tacgctctgc caagactgct tcaagcttat cgatctcctt
    10381 ttgccttcta tctcgctgct ctttggcttt tgccaactcg gcttttaatg tttctcgctg
    10441 agcagataat tcgttaattt gtttttccaa actttcttct gtgacttcat gatcagaaaa
    10501 gtctgctgtc aatgtcgcca actgcttttc tatcgcttct tgacgttcgg ttgcctctgc
    10561 tttttggact cgggcaccgc ggagctggat ctgcagatga ttaaactgct ctttcaagac
    10621 agcaagatct gcttgttctt gtgctttttg actttgtact tgtgctcttc tggcttccat
    10681 ctggtcgctt tcttgactta acgatttgat ttcttcgtcg attttttgtc gttgcgattc
    10741 aagatctgtt tgtttgtctt tcaattcgtt cgtttgcttt tcgtaatctt cgataaactg
    10801 ctgcagttca cgtgtctcaa aagttgagat ttgcttttct ttttcaaaac gttcaagttc
    10861 atttatgatg ttttgtagct gatttgtcgc ttcttgttct tcaaaacgta attgttcgcc
    10921 tcgtgtacgc aaaacttctt gttcttcggc taaacgagct gtttcttgtt gcagctcctg
    10981 aacttttttt tcttgtgctt ggagttgttt atctgcttct tcaaattcac tagtcaactg
    11041 tttcaattct tgattttgta caaacaaact gccttggttt cctcgtttat tcgctccacc
    11101 tgtcatggat cctccggcat tcatcacatc accttctaag gaaaccactc gatattgata
    11161 gcggatcgtt tgcgcgattg cattggcact agttaagtct ttggctaaaa ggatcgttcc
    11221 tagcaggttt tgaaccacag tttggatttg atcgggataa ctgacttgtt cactggcaat
    11281 ccctataaac ccttctacag ctgctgcttg tgtcaatata tgagccggta attgtcttgg
    11341 tttgattgtc gtcagtggaa ggaatgttgc tcttcctcct ctttgctgtt tcaaatacgt
    11401 gatggcttgc cgagcatctt tttcattttc aacgatgaca tgctgggctg ccccacctaa
    11461 agctgcttcg atagctaacg tgaaatctgc aggaacatcg atcagttcag ccactgctcc
    11521 gacaataccc gaaagctgct gtttatgctg caagaccaat cgaacccctt ggtaaaaacc
    11581 aaaatagttt tcttggattt cttgaaggct tttcttacga gcacgtagct gctggacttg
    11641 attcattaat tgatacattt taggctgttc ttttgctaat gcttcttgga actttttccc
    11701 gttttgttgg atcatttcta acttctcttt tgtttcagtt aaagatgatt ttagctgcgc
    11761 ctgcttttgt gtcaattctt ctttttgtaa gatcaatcgg tctactgatg cttctacttc
    11821 cgattgcttg gctaacgtct gcttgctttt tgctgtttct tgtatatatt gacgttctag
    11881 atacttcagt tcgtttccta cagctgcttg ttcttgcatc aaatcaacat attgatctct
    11941 taattctgcc aataattctt tcgttgattt ttggtatttt tctaattctt gctgtgtttt
    12001 tatgagattt gcttcagctt tttggatctc tgtttctttt tctgctgctg ctttcatcaa
    12061 gctttcttga agcttttcaa aatgtttaac ctttttttgt gcttctgcta atgaagtttg
    12121 atattcttgc gagctttttt gtgtatgctt tgtccgctcc tgcaaaacat ctttttgtcc
    12181 ttctgtctgc ttcagttttt ccgaaagatc taataatact tgctgatttt tttcgatcag
    12241 acgatcggct tgcgcatttt ctttacgctg cttcgccaaa atggattctt gttcttggat
    12301 cgactcagat agtttgccta attctagatt gaatttcgca agctgtgctt gtttgttgtc
    12361 ccaatctttt tttgctgtct tgatttcagc aaccatcaaa gagacatcgg tttgagtcag
    12421 tgtttctttt agccgtaaaa actcctttgc tgcctcactc tgggcggcca aaggagttaa
    12481 ctgttcttcc aattcgtgga tgatgtcttg gacgcggctg aggttgtctt ctgtttcaaa
    12541 aagcttctgt tctgctttct ttttccgctg cttgtatttc aagacaccag ctgcttcttc
    12601 aaagatccca cgacggtctt ccggtttact actgaaaatc gcttctactt ttccttgaga
    12661 aatgattgag aacgattctt ttcctaatcc agaatctaag aacaattctt ggatatcttt
    12721 taatctgcag ctttgtttat tgatgaaaaa ctcgctttcc ccagtccgtc gataacggcg
    12781 ggttacactg atctcattgt actccaatgg cagataatga tcactattgt ctaaaatcac
    12841 tgtgacttct gcaatattca gctgcttacg ggtatcggaa ccggcaaaga tgatatccgg
    12901 catttttccg cctcgcagac ttttagcaga ttgttctcct aatacccagc ggatagcctc
    12961 tgtaatattg ctttttccgc tgccatttgg tccaaccact gctgtgacgc tattttcaaa
    13021 atcaatgact gtcttgtctg caaatgattt aaaaccggct atttcaattc gtttcaaata
    13081 caaagctttc cctcacttac ttttacagtg ctgccaatgc attttccgcc gcagcttgtt
    13141 ctgctaattt tttcgatttc ccttgtccag tgccaatcaa ttgatcatct acataaactt
    13201 cgatccaaaa tactcgttca tgagctggac cttcttcatt gatcaatcgg tattcgattg
    13261 atacatcacc tgatttttgc aagacttctt gtagttttgt tttatgatcc atctcatgtg
    13321 aaaaagcacc tgctttgact ttaggaaaaa tcactttttc gatgaatgca tgggtcgtgt
    13381 cgaatccctg gtcaagatat aacgcgccta aaaaagcttc gaataggtcg caaagcaatg
    13441 ctggtcgcgt acgtcctcct gaattctctt ctcccttgcc caacaaaaca tattgatcaa
    13501 agtgacattc tttggcaaat ttcgacaagc tgtcttcgcg aacgatcgca gctcgtgttt
    13561 ttgtcaattt tccttctggc aaatgaggat ataaacggaa taaatactga gaaaccatca
    13621 gttctaaaac ggcatctcct aaaaattcaa gtcgctcatt atccgataat tgcagattgc
    13681 gatgctcatt cacataggat gaatgggtaa aagcttgttc aagcaaattt agatcgtgaa
    13741 aaacgatgtt atagttttct tttagttctt ttgtcagctg gttgtccatt ttccacattc
    13801 cttttatgga tattttttcg atacttcctt attatactgg ttttttgtca aatttgaaat
    13861 ggttcggtca aaaggtaagt aaagctttaa taatcctagt gattgacgag aagttgtgaa
    13921 aaattcgttt agtttcgagt atgaagaaaa aattttgtga aaatggcttt ccatattttt
    13981 gcataaattt gacttaatac cgagaaactg gactttgaac accgtttatt ggagaagtca
    14041 tgaaatcagc gccttgacct gaatattaga gaaataaaca gaaaaatatc aaaaaaccga
    14101 aaaaggacat cgcccttttt cggtcatttt tagtgattat gaacgtgaat ctgctgtaac
    14161 agaaacattt gcccatgggt ttggtacatc ataagaccaa tcccagtctt tgacacggtc
    14221 gttgactggc aatacttcat tacggaacaa cgttggaatc gcaaatgctt cttcaaatgc
    14281 atattgttgc catttttgat atgcttcttt ttggtactct tcatcaaatg ctttgcttga
    14341 actgatatcg ttgatcaatt ttgtattttc ttctgattca taacgagtgt agttgaacgc
    14401 agcatttggt ccccatagtc cacttggatt tggatcagtt cctgtattcc aagcagcttg
    14461 gtaaacatca atttcaggat catcgttttc taatttatca tagaaagaat tgaattcgat
    14521 cagacgacct gttgtgtatt ggacatccaa accaatttct ttccattgtt ggatatagta
    14581 atctgccaat ggttgcgctg tttccccacc ggacatcgaa gcaaagttga ttgttagttt
    14641 atttccatct ttgtcttctc ttaaaccatc gtcatccaca tctttgtagc cagcatcatc
    14701 taatagtttt ttcgctttgt ctagatcttg tgtgaatcct ttcatctcat catcatggta
    14761 tgactggaat actggaggaa tcaatgaagt cgcattgctt ctcaaacctg catagaaacg
    14821 ttcacctact gcattattat ctaatgcata gcccattgct tgacgtaatg atttatcagc
    14881 catttttgaa tctggattgt attccacact ctttgtatct gcattccatt tacctaactt
    14941 gaatcctaga taagtataag aagtttgttc acggcctaag tttgtatagc cagcaatttc
    15001 tttataggta tcgtaagtat ctgttggcat ttggaaaatc atatcatata atttagagtt
    15061 caatgcttca gttgcacttg ccatcggtac agatttcatc gtgatctttt tcaatttagg
    15121 cgtaccattc cagtagtgct cgtttggcaa gtattctact gattctcctg caacgatatt
    15181 actcatgtaa taagggccaa aagtcactgg atttttacgt acttgatcgc ttgattccat
    15241 atctttgatt gctacacctt ctaacgtatg ttttggtgct gcatatgacc agataccacc
    15301 acccgattga agcatgcttg ggtttacttc tttatattcg atttcaactg ttttgtcgtc
    15361 tatttttttg attccggaga ttgtgtcggc acttccatca tgatattctt ccatacctac
    15421 gatgttacgg aaagtgtcgt cataacggat accagtgtaa tctttattcc caatgatttc
    15481 gtaagggaaa atcacatcgt ccgccgttac atcttcacca tctgaccatt tgacgttgtc
    15541 ttttactttg attgtcgctt ttttcgtatc ttcatctaga tcaagcgttg cagctccgtc
    15601 gtctgtgatt ctgaaatcgt catcgtttgc aaatagcggt tcatgagaag gagccataaa
    15661 agctgcatca tatgaatctt ggtaaaactc ccactggaac aatcctttaa actgactgtc
    15721 cgttgcgacc gcagcttcta attcgccgtc ttcgattgca tcatcattat tttttgttgc
    15781 aattggaaaa gttaattcct ctttgttatt gccgttgcct ccagagttgt ttccagaggt
    15841 atctgtacct ccgcccccgc atgctgcaag aacgacagca gacatcagcg ctgcaaatcc
    15901 aaaaatcgtt ttcttcattc ctatttcccc cttcgcttct atactagcct aaccgttggc
    15961 gcgcatctgc agaacgcttg aatgcttgtc caacataatt gatactcaac atcaaaacta
    16021 aaataaggat tgatgcgggt aaccagatcc attgccgatt cactaagact tctccgttac
    16081 ttgcataacc gatcaatgta cctaaactag gtacatttgc cggcaagccg aaacctaaga
    16141 aagtcaaagt tgtctcgata ccgatgtttg ccgcaaagtt catcgtcaaa ttagtaatga
    16201 ttaatgaact tagattcggc atgatttcgc ggaacatgat tttaaaatca ttggttccca
    16261 ttgtttttga ggcactgaca taatctcttc ttgcttcaga taatgtcttg cttcggaata
    16321 atcgggcttt agctacccaa taaaatgcac tcatgatgaa tataaaggac cagacattgt
    16381 atctggaaat aatcgatacg aagacgataa tgatcagcat gatcggtaga atcatgataa
    16441 aatcaacgat ccgcatcaag atgttgtcaa tgatccctcc gtagtagcca gagatgatcc
    16501 ctaatccaac cccgatgatc gaagtaagga tcgtgatcgc aaatccgatc gtaacagagt
    16561 ttctagcacc tatgatcaat tgtcctaata catcacgtcc gccttcatct gctcccagaa
    16621 tgaacttttg tccttgtgtc gaaactgttc ctggtgcagc gtatttgtcg aagatactaa
    16681 catacatcac tttgtcttga tcggtcagca tcgcaccgat aaagatcact aataataaaa
    16741 ccactagcaa acctaatgaa aaaatagcaa ttttatcttt tttaaattct cgagcaatca
    16801 ttcgtatccc cataggagga atactttctt gaactgctgt attgtttttt tcactcatcg
    16861 atccattctc ctttctgatt ggttttattg aatccgaata cgcgggtcaa cgatactcat
    16921 gatgatatct gaaagtaatg tccccactag cgtcgcgatt cctaaaatca atactaagga
    16981 tgtgatcacg gtatagtccc gttggccgat ggaatcaata aatagttttc cgactcccgg
    17041 ataaccaaaa atcttttcga taactacaga accagcaatc aacgaagtga tttcataccc
    17101 catttgagaa gccatcggca acgctgcatt tcggaaaata tgtcgactga atactttatt
    17161 cgtcggtacc cctttggaac gggctgttct gacaaaatcg agcgatttcg aatcgattac
    17221 ttcacttcgc agatattgga ttgtgacggc agtcgccaaa aatgcttggg taattgatgg
    17281 taacagaata tatttcaaac ggtctatcca ttgagtgata aatccacctt gcaaacctag
    17341 tgtttgtgat ccacttgttg ggaaccagcc taaacgataa ccaaaaacaa atagcatgat
    17401 caagccgaag ataaataaag gaacagcaaa actaaagaag ttgtagatta cgacaatctt
    17461 atccaagatt gaattttgat accggccagc aagcatcccc agaggcacag caatcaaata
    17521 agtaaggaca actgtcaaga tcgatagata taaagtgttg atgatccgtt cgccgatcaa
    17581 gtcagcaaca gagcgtttgt ataagaaact ttgaccaaaa tcgccttgca aagcatttcc
    17641 gatccaacgg aagtactgga cataccatgg gtcattcaag ccagctgctt gtcgcatctg
    17701 ttcgatcact gccggatcct gatttggatt gatcaaaccg gtaaatggat cccccggcat
    17761 cgcttgggct aataagaaga tcagaacact taaaataata acttgcggga tcatcaataa
    17821 aacacgtcgc agaatcgttt tccacattag cttgcacctc catctttcaa cgccactttg
    17881 tgtgtagttg tgatcgtacg caaatcgtag actcgaccag tcgcatcata gtattctttt
    17941 tggttttgga tatactcttg ttctactctt cgtcgatttt ctttgtggct ttccctattt
    18001 tctacatcga tttgcggaat agctgaaagc agacgtttcg tatagatatg tcgcgggtca
    18061 ttataaatgt cttctcttgt accgatttct acaaatcgac ctttgtacat aattgcgata
    18121 ttgtcgcaca tgtgttttac tacacctaaa tcatgtgaaa tgaataaata gctgaggccg
    18181 tattcttgct ggattcgttt catgaaattc aatacttgcg cttgaacaga tagatccaga
    18241 gcagatacag gttcatcggc tacgatcagt tttggattgg tagcaacagc acgggctact
    18301 cctagacgtt gacgttgtcc gcctgaaaat tcatgaggat atttatatag cgcatcttcc
    18361 ggcatcccaa cgatatctag cagtcctttt acctttttct tttcttcttg atcgcttaac
    18421 cgttcaaaat ttcggatggg ttcagcaatg atatccaaca ctcgcttttt aggattcaaa
    18481 ctggacattg aatcttggaa gatcatctgc acatccttgt tataacccat ttttttgcgg
    18541 ttgcttcgct tcgttacatc ttttccttca tacatgattt gtccggctgt taccttttct
    18601 aatcccacaa cggcttttcc cgtagtagat tttccagagc cagactctcc cactaaacca
    18661 tatgtctttc ctttttcgat aataaaatcc acaccatcta cagcatatac atgatccgtt
    18721 actcgattga agaatccact gcgaataggg taatgaactt tcaggtcttt gattgtgatt
    18781 aattctgtca tctacacctc tcctttttcg tctctaaagt ggaaatgctt atagcaggag
    18841 caacgtacca agtggttcgg tgcgacttca tgaaggatag gctcttcttc atgatcgctt
    18901 gctggtatcc atggaatacg tggtgcaaac cgacaacctt tacgaggcat gtttttcaga
    18961 gaaggaacga caccttcgat cacatggagt tctgcatcat gatttccttc ttgtggtata
    19021 gagttcagta aagagcgtgt ataaggatgc ttaggatgat caaacagttc ttttacacct
    19081 gctacttcaa cgaactgtcc gccatacatc actgccactc gatcagccat ttctgctacg
    19141 actcctaagt catgagtgat caagatgatc cctgtttttg tttcttcctg aaggtctttc
    19201 agtaaatcaa gaatctgtgc ttggatggtt acatctaggg ctgtcgtcgg ttcatctgca
    19261 atgacgattg gaggtttgca ggcgattgcg attgcgatga tgacacgttg tctcataccg
    19321 cctgatagtt catggggata ttggcgtcca actctcgcag gattaggaat ccctacttgt
    19381 tctagcaatt ctagtgcacg agcttgacgc tgttctgcat tcatctttgt gtgataggaa
    19441 agtccttctt tgatctggtc ttcgattctc atgagaggat ttaatgcaga caacggatct
    19501 tggaaaatca ttccgatatc atttccgcgg attttattat agagcgtttc gttcaggtcg
    19561 attaaattca gatcgttata taaaatctca cctgtaattt ttgtgttatt aggatcaagt
    19621 aatcccataa tcgtagtagc caaggtactt ttcccgcacc cagattcccc aacgattgct
    19681 aaaatctcgt ttttgtctaa agtaaatgaa acgtcatcta ctgcatcgta atagtcatct
    19741 ttgagtcgga atcctgtatg taggttctga acttctaata attggttatc ggacaatcga
    19801 atacccctct ctgcttccta aatggaagtt tttcttaata aaaaaggaca ttacacagaa
    19861 taaagcgttt tataa
//
LOCUS       DAZUKA010000046        19742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000046 DAZUKA010000000
VERSION     DAZUKA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.046"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..506
                     /locus_tag="ACG8PN_001282"
     CDS             24..506
                     /locus_tag="ACG8PN_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861446.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            703..1593
                     /locus_tag="ACG8PN_001283"
     CDS             703..1593
                     /locus_tag="ACG8PN_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS4861447.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1919..4393
                     /locus_tag="ACG8PN_001284"
     CDS             1919..4393
                     /locus_tag="ACG8PN_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4861448.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4496..6232)
                     /locus_tag="ACG8PN_001285"
     CDS             complement(4496..6232)
                     /locus_tag="ACG8PN_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4861449.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6229..6933)
                     /locus_tag="ACG8PN_001286"
     CDS             complement(6229..6933)
                     /locus_tag="ACG8PN_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4861450.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7064..7567
                     /locus_tag="ACG8PN_001287"
     CDS             7064..7567
                     /locus_tag="ACG8PN_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4861451.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7527..7865
                     /locus_tag="ACG8PN_001288"
     CDS             7527..7865
                     /locus_tag="ACG8PN_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4861452.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7886..9304
                     /gene="ffh"
                     /locus_tag="ACG8PN_001289"
     CDS             7886..9304
                     /gene="ffh"
                     /locus_tag="ACG8PN_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4861453.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9416..9994
                     /locus_tag="ACG8PN_001290"
     CDS             9416..9994
                     /locus_tag="ACG8PN_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4861454.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9998..10519
                     /locus_tag="ACG8PN_001291"
     CDS             9998..10519
                     /locus_tag="ACG8PN_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4861455.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10598..11152)
                     /locus_tag="ACG8PN_001292"
     CDS             complement(10598..11152)
                     /locus_tag="ACG8PN_001292"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4861456.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11405..11680
                     /gene="rpsP"
                     /locus_tag="ACG8PN_001293"
     CDS             11405..11680
                     /gene="rpsP"
                     /locus_tag="ACG8PN_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4861457.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11692..11943
                     /locus_tag="ACG8PN_001294"
     CDS             11692..11943
                     /locus_tag="ACG8PN_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4861458.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12067..12858
                     /locus_tag="ACG8PN_001295"
     CDS             12067..12858
                     /locus_tag="ACG8PN_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861459.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13028..13549
                     /gene="rimM"
                     /locus_tag="ACG8PN_001296"
     CDS             13028..13549
                     /gene="rimM"
                     /locus_tag="ACG8PN_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4861460.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13539..14300
                     /gene="trmD"
                     /locus_tag="ACG8PN_001297"
     CDS             13539..14300
                     /gene="trmD"
                     /locus_tag="ACG8PN_001297"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4861461.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14436..14783
                     /gene="rplS"
                     /locus_tag="ACG8PN_001298"
     CDS             14436..14783
                     /gene="rplS"
                     /locus_tag="ACG8PN_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4861462.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15093..15335
                     /locus_tag="ACG8PN_001299"
     CDS             15093..15335
                     /locus_tag="ACG8PN_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4861463.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15423..15569)
                     /locus_tag="ACG8PN_001300"
                     /pseudo
     CDS             complement(15423..15569)
                     /locus_tag="ACG8PN_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15626..15940
                     /locus_tag="ACG8PN_001301"
                     /pseudo
     CDS             15626..15940
                     /locus_tag="ACG8PN_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            15969..17228
                     /locus_tag="ACG8PN_001302"
     CDS             15969..17228
                     /locus_tag="ACG8PN_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4861464.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKVVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17281..18177
                     /locus_tag="ACG8PN_001303"
     CDS             17281..18177
                     /locus_tag="ACG8PN_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861465.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18188..19057
                     /locus_tag="ACG8PN_001304"
     CDS             18188..19057
                     /locus_tag="ACG8PN_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861466.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19067..19742
                     /locus_tag="ACG8PN_001305"
                     /pseudo
     CDS             19067..19742
                     /locus_tag="ACG8PN_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6467 a         3448 c         4097 g         5730 t
ORIGIN      
        1 aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat
       61 ttttttggtt ttttcctatc gttttgctac cttttacatg ggtaaatgct gttgtacaag
      121 gtgcggagat attaggcctg atccaaggaa gcggattagt ggtttctagg aattttccag
      181 ctttgactgt tttctattta gtgataagtg taggtctctt attaccaaaa tctctgatca
      241 atcactacaa ttggcagctg ctttactttt cttgcttatt agtatttaca tctatgtttt
      301 ctttgctgcc tcgcttgatt atcggcccta atggtttgag cgagagtgga ttgaaggatt
      361 cagcttattc ctccatgtat ttcttgacga tcaaaccatg gttctattta gtaattgcct
      421 catttattct tgcaacattt ttttacttct ggacagaaat caggataaaa agtgaacgat
      481 ctgtatctat aaaaaataac tattgatggt ggatcatcag taaaaaagca tatgagctgc
      541 gtaaaatgca tgtttctcat atgctttttt tattattatg taaagaatgc gaaaaacgtt
      601 tacatgattt ttacataact taactatatt tttacagcaa ccatacgagg gatttacagt
      661 cgactgataa actttagttg taaataagaa aggagcaata aaatgaaaaa agtattgttt
      721 actctaggta tgatgggaat tcttttagcc ggatgcggct caggatcagg aaatacagat
      781 ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta
      841 ggttcgacag cattacagcc gttagtcgat gcagcacaag aaagctttgt acaagaaaat
      901 ccaaattatc aaatctctgt acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa
      961 gctggagcag ttactatcgg aaactctgat gtatttgcag aagaaagaga cggagttgat
     1021 gcttcaaaat tagtggatca taaagtagca gtggtcggga tggcaccaat cgtcaataaa
     1081 gatacagatg tcaaagatat caccaaacag gaattgattg atatctttac aggaaaaatc
     1141 actaactgga aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac
     1201 ggcagcggta cccgcgcgac attcgaaaaa tggggactgg atggtgctac ccctgtgcag
     1261 tcccaagaac aagattcatc aggaacagtt cggcagctgg taagtcaaac accaggagca
     1321 atcagctatt tagctttctc atatctcgat gattccacac aagcactaag tatcgacggt
     1381 gtagaaccaa aagaagagaa tgtagcagat aacagctggg gaatctggtc atatgaacat
     1441 atgtacacga acggaaaacc ttcccctgaa gtccaaaaat tcttagacta catgatgaca
     1501 gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt taccaatcac aatgatggaa
     1561 gtagaacgtg atcacgaagg aaatatcaaa taactagcag ataaacatgc cacggtagtc
     1621 gttactatta aggaaaaatc aactatgttt ccccacatat acatttttcc tttaattgtg
     1681 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     1741 caccgtttat tcgaaaaaaa gagctacgac atagaactgt cgtagctttt tttttgctat
     1801 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
     1861 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
     1921 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
     1981 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     2041 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     2101 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     2161 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     2221 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     2281 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     2341 tgtaatagat gacattgtta ttttagagac tggacatcaa attccagcag atgcagaggt
     2401 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     2461 caaagaaata ggcgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     2521 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     2581 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     2641 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     2701 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     2761 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     2821 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     2881 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaag tgatcgtctc
     2941 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     3001 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     3061 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     3121 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     3181 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     3241 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     3301 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     3361 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     3421 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     3481 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     3541 aaagaaaatc ttcgttcaat tgctaaagcg tcgtaacaat acagtagcta tgacaggtga
     3601 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     3661 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     3721 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     3781 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     3841 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     3901 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     3961 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     4021 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     4081 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     4141 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     4201 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     4261 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     4321 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagttaaa aaatttctat cgatatttaa
     4381 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     4441 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     4501 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     4561 taccatgaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     4621 aatgccggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     4681 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     4741 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt
     4801 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     4861 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     4921 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     4981 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     5041 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     5101 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     5161 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     5221 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     5281 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     5341 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     5401 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     5461 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta tacgcccgat
     5521 acatcttact catttgttcg ctcaacgcat tgatcgtctg atacaattct tcccattgat
     5581 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     5641 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     5701 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     5761 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     5821 tgactggtaa tgcgacatat aaaagatccc cattcaaagt agtacttttg cgcaaagcgc
     5881 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     5941 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     6001 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     6061 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     6121 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     6181 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     6241 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     6301 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     6361 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     6421 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     6481 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     6541 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     6601 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     6661 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     6721 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     6781 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     6841 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     6901 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     6961 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     7021 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     7081 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     7141 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     7201 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     7261 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     7321 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     7381 cccttcatgc ggaacccatt cgatttatga cgggagtttt tccggaataa aaaaaaatgg
     7441 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     7501 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     7561 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     7621 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     7681 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaacttcatc
     7741 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     7801 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     7861 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     7921 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     7981 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     8041 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     8101 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     8161 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     8221 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     8281 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     8341 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     8401 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     8461 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     8521 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     8581 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     8641 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     8701 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     8761 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
     8821 tgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
     8881 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
     8941 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
     9001 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
     9061 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
     9121 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
     9181 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
     9241 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
     9301 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
     9361 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
     9421 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
     9481 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
     9541 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
     9601 cgatattaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
     9661 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
     9721 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
     9781 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
     9841 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
     9901 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
     9961 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
    10021 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
    10081 cactccaaca ggtaagcaaa ccacttatga agctttttat gaaacaatcg atacgaccat
    10141 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
    10201 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
    10261 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
    10321 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
    10381 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
    10441 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
    10501 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
    10561 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
    10621 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
    10681 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
    10741 gcacattcaa aaagacaagt gattgtcgta gatggtggtt tgcgccaaag ccgccgatcc
    10801 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
    10861 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
    10921 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
    10981 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
    11041 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
    11101 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
    11161 tcctccttca aactatctat aacaatctta caaaaaataa gtaactggag caaataaaaa
    11221 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
    11281 aaaatcttct atttcctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
    11341 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
    11401 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
    11461 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
    11521 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
    11581 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
    11641 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    11701 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    11761 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    11821 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    11881 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    11941 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt tattaattga
    12001 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    12061 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    12121 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    12181 agtccagaaa aaagtagtac caaaacggct accaccactg aacaaacaat ggctacctca
    12241 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    12301 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    12361 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    12421 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    12481 acaatccaag ttttgcagcc ctttgagatc gacacaaatg gtcagcaagt ggacttaggt
    12541 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    12601 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    12661 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    12721 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    12781 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    12841 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    12901 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    12961 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    13021 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    13081 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    13141 gttaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    13201 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    13261 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    13321 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    13381 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    13441 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    13501 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    13561 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    13621 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    13681 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    13741 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    13801 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    13861 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    13921 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    13981 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    14041 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    14101 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    14161 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    14221 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    14281 cagcagatga agccaagtga ctttttctaa aatattcttt acagtaggtt gataatatgt
    14341 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct atgagaaaag
    14401 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    14461 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    14521 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    14581 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    14641 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    14701 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    14761 gtatcaaaga aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc
    14821 aagggttctt ttttcttagc atgtaatttg aacaccctga tccagttttt ttctttaatt
    14881 attggtaggg gagaataatg gattagaata aatctaccta taagtagtag taaaaaaaag
    14941 agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc agaatattct
    15001 aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat acttatacgg
    15061 agcaagaaaa aatgaaagtt tgagacacat tcatgaatga tggaaaagta aaacaagtac
    15121 caagttcaac aaaaaagaaa aacatcctat tgaaagaagt cttgaaacgc tttgaccatg
    15181 gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt tctagtggcg
    15241 attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga agttcagacg
    15301 gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata ctcattgaaa
    15361 aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt tgtcatctgt
    15421 atctataata caaagccaag aacgttattc aaaatatgga tagctatgat aaaagctaat
    15481 gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc gtacatgaaa
    15541 aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta cgaataaata
    15601 ccaaaacgta cctcctaaaa ttagattcaa agaagatagc aaagaggaaa aagatagttc
    15661 tgcagcagct atagcggcat gtggattgct agaactttcc aaacaactgc ctgtttcaga
    15721 tcctcgtcat ttcgattacg aagagatagc aatcaaaatc ttggaagaat tacagaaaaa
    15781 ctatacaacg aaacgagtta acggatcaaa cggtctgctt ctccatgccg tttatgataa
    15841 aaacagctta aaaggtgtag atgaatgtgt gatctggggc gattactttt atgtagaagg
    15901 aatcactcgt ctagctaaaa catggtactg ttactggtga tgtataagag aagaggagga
    15961 ttcatataat gaagcgatgg aaaaaaatga tgattggatc aatagtttta ggaatgtcac
    16021 tcgtatttgg tgcttgtggt aatcaagaag acagtagtga agaatcagca aatgaaatta
    16081 ccgtttcgac atggaattat gaaacaacac ctgaattcaa agcgctcttt gaagcattcg
    16141 aaaagaaaac gggaatcaaa gtgaaagtag tagatattgc ttcagatgat tatgatacga
    16201 aactcaccac gatgctttct tctggagaca cgacagatgt attgacgatg aaaaatcttt
    16261 tgtcgtattc aaattatgct ttacgagatc agttagtcga tcaaacagaa cgaattagag
    16321 aattagacac tgatgctgct gaagaaacat atgaaatgta tgatatcgat ggaaaaacat
    16381 atgcattgcc atatcgaacg gacttttggg tactatatta caataagaaa atgtttgatg
    16441 atgcaggaat cgaatatcca aaaaatctaa cttgggacga atatgaagat ttagcaaaaa
    16501 aacttagtaa aaatgacgga caagtttacg gagcatatca acatatctgg cgttcgacaa
    16561 tccaagctat tgcagctgct caaaacgagg cgaatcttgt tgaaccagat tatcaatata
    16621 tgactgatta ttatgatcga gcattgagga tgcaaaaaga aggcgcgcag atggattttg
    16681 gcacggctaa atcaacgaaa gtaacctatc agtctcaatt tgaagaacaa aaagcagcaa
    16741 tgatgtacat gggaacttgg tatatggctg gcatactcgc aaacaaagaa gcaagtaaga
    16801 cagaagtaga atggggaatt acagctattc ctcagaagaa aaaaggagaa tcggtcacga
    16861 cgtttggctc accaacagca tttgctgtca ataaaaactc aaagaaacaa aaagcggcgc
    16921 aagaatttat tgaatttgca gccagtgaag aaggagcaaa agtattagca ggagtaggag
    16981 tagttccttc atatcgtacg gatgagattg atcagcttta ttttaatctc gctggaatgc
    17041 caacggatga aatttctaaa aaagcttttt ctcctgatga aatcaaactt gaatttccga
    17101 ttgatacgta tgggcctgca atcgataaga ttttacaaga agaatatgac ttagttttgg
    17161 ttggagatga aacgccggaa aaaggaacgg taaatatgga gaaacgtgta gctgcggaaa
    17221 aagaataatc atgaaaggag agaagccaag agataaaaca ctcttggttt cgtcatcgaa
    17281 atggagaata gtataaataa gaaagctaca aatcatttgc gtagaaaacg aacgtggatc
    17341 gcttattcgt ttatcgcccc gaatttcatt ggttttttcg tttttacatt aataccaatc
    17401 ctgttttcgc ttgtactagc atttatgaaa tgggattctt tttcaacgcc agaatttgct
    17461 ggactagata actttcgtaa aatggccaac gatgagacat tttggatttc tttgaaaaat
    17521 acttttttat ataccgctgc tgtagtgcct ggcacacttg tttgttcgtt agggctagct
    17581 ttactactaa atcagaaaat tagagggatg aagctttttc gtacgatatt tttctttccg
    17641 tatgttactt ccttagtagc aattgctgtt gtttggaata tgttgtttca tccaacagtg
    17701 ggacctatta atatcttttt aagacgattt attgaacatc ctccaggttg gacttcaagt
    17761 tcaaattggg cgctttgggc aattgtaatt gtcagtattt ggcgagggat gggttattac
    17821 atgattctat accttgcagg actacaaagt atccaaaaag aactatatga agcgtcttcg
    17881 ctagatggag caaaccgttg gaaacaattt ttgaatgtga cattaccttc gttgagacca
    17941 acaaccttct ttgtaacgat catgcttgtc atcaattgtt tcaaggtgtt tgaccttgtg
    18001 caagtgatga cggatggcgg ccctggtcga gcgacaaatg tgctggttta ccaaatctac
    18061 aatgaagcat tcaaaaaatt tgattttggt tatgcttcag ctatttcact tgttttgttc
    18121 gtaattgttt tgggcgttac ggttgttcaa ttttcttgga ataagcgtcg tacttaggag
    18181 gtgtgatatg gaagagagtg ttcgaaatga aagaaaaaaa gcaaaaattc gttcaaaaaa
    18241 catgcccttt attcaatggt tgggtatgat tctgctgatt gtattagctt tattgacgat
    18301 cattcctttt ttatggatgg tttctgcttc attcaaagca aataatgaag tgttaacgat
    18361 tccgattcaa tggatcccta aaacttggca tttagagaat tatctggtta tttggtctcg
    18421 tattccttta gtgacatttt ttaagaatac agctttttta agcgttgtga tcacgttgat
    18481 tcagttgttt acttctagtt ttgctgttta cgggttttct gaactaaatt ttaaagggcg
    18541 cgatatttta tttttagcat atatcggcac gattgctgtc ccttggcagt cttatatgat
    18601 cccgcagttc attatgatga gaaaagtggg tttaacagat acgttgtggt cgttggtgct
    18661 attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag caatattaca gtagtatccc
    18721 agattcttta tgtgagagtg cgcggatcga tggcttatca gagtgggaaa tctatcgcaa
    18781 gatcatttta cctcttacaa aaccggcgat tgccagtttg acgatcatca cattcgttaa
    18841 tacatggaac gattatatgg ggccattcat ttatttgtct tctacggaaa ataaaacaat
    18901 ccagttaggg ttgaaaatgt ttattgggct atttgatgca gagtacgcat taatcatggc
    18961 tgcctcagtt gtttctgttc ttcctgttgc ttttatattt ttagcgatgc aaaaatattt
    19021 tgtagaaggg atcgcttcat caggaatgaa ggaataggag gtgattatga aagagcagcg
    19081 ttttgacaaa aatgtttata tggaattctt ttattggttg tatcaatcat tggcaagtag
    19141 cctttgtttt tggtgtgtca atatcttgta tatagcagct gcgttccttt tgtcgataga
    19201 tggaaggaat gtcttttcct ttattttttc tttacttttt attggtccag gttgcatcgt
    19261 gatgataggt atgacagaag aattagcaaa agaaaaaagt ttttcaccag tacgtttgtt
    19321 cataaagacg tttcgccaat tttggctaaa gggatttttt tattggctat ttgcttggat
    19381 agttagtgtg ataatgattt ttgattgttt cttttttata cgtttttctt atggaaaatg
    19441 gttgattccg ttgtttgttt tacttgcttg ttttacttgc ttgtttatca gtttccttta
    19501 gcatcaactg ctggtatttt caggtgagaa atccagcgtc gaaaccaaat caagttctac
    19561 ggatagcgtt ctactatacg ttgaaaaaat ggtatgtgag tttactagat tttttactat
    19621 tgacaagttt gtttctcttc tttttcgtaa agccccaatg gtgtattcta ctaggtcctt
    19681 ctatcgtatt tggtttgatt tacttcaaca atcgtaaatt gatgagaact atggatctgt
    19741 ag
//
LOCUS       DAZUKA010000047        19631 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000047 DAZUKA010000000
VERSION     DAZUKA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.047"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8PN_001306"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8PN_001306"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4861467.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG8PN_001307"
     CDS             complement(1174..1869)
                     /locus_tag="ACG8PN_001307"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4861468.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG8PN_001308"
     CDS             complement(1882..2598)
                     /locus_tag="ACG8PN_001308"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4861469.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG8PN_001309"
     CDS             2928..3536
                     /locus_tag="ACG8PN_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4861470.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG8PN_001310"
     CDS             3861..4184
                     /locus_tag="ACG8PN_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4861471.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG8PN_001311"
     CDS             4366..5736
                     /locus_tag="ACG8PN_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4861472.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG8PN_001312"
     CDS             complement(5866..7167)
                     /locus_tag="ACG8PN_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4861473.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG8PN_001313"
     CDS             complement(7521..9512)
                     /locus_tag="ACG8PN_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4861474.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG8PN_001314"
     CDS             complement(9656..9922)
                     /locus_tag="ACG8PN_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4861475.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8PN_001315"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8PN_001315"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4861476.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8PN_001316"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8PN_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4861477.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG8PN_001317"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG8PN_001317"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4861478.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG8PN_001318"
     CDS             complement(14082..14603)
                     /locus_tag="ACG8PN_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4861479.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG8PN_001319"
     CDS             complement(14606..15448)
                     /locus_tag="ACG8PN_001319"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4861480.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG8PN_001320"
     CDS             complement(15441..16166)
                     /locus_tag="ACG8PN_001320"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4861481.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG8PN_001321"
     CDS             16342..16638
                     /locus_tag="ACG8PN_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861482.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG8PN_001322"
     CDS             17206..18798
                     /locus_tag="ACG8PN_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4861483.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG8PN_001323"
     CDS             18811..19551
                     /locus_tag="ACG8PN_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4861484.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5618 a         4028 c         3724 g         6261 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt c
//
LOCUS       DAZUKA010000048        19428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000048 DAZUKA010000000
VERSION     DAZUKA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.048"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..635)
                     /locus_tag="ACG8PN_001324"
     CDS             complement(39..635)
                     /locus_tag="ACG8PN_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4861485.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="ACG8PN_001325"
     CDS             985..1329
                     /locus_tag="ACG8PN_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4861486.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="ACG8PN_001326"
     CDS             1330..1749
                     /locus_tag="ACG8PN_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4861487.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="ACG8PN_001327"
     CDS             complement(1841..2191)
                     /locus_tag="ACG8PN_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861488.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="ACG8PN_001328"
     CDS             complement(2157..3488)
                     /locus_tag="ACG8PN_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS4861489.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="ACG8PN_001329"
     CDS             3743..4309
                     /locus_tag="ACG8PN_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4861490.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="ACG8PN_001330"
     tRNA            4454..4541
                     /locus_tag="ACG8PN_001330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="ACG8PN_001331"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="ACG8PN_001331"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4861491.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="ACG8PN_001332"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="ACG8PN_001332"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4861492.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8712)
                     /locus_tag="ACG8PN_001333"
     CDS             complement(8266..8712)
                     /locus_tag="ACG8PN_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4861493.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8940..9089
                     /gene="rpmG"
                     /locus_tag="ACG8PN_001334"
     CDS             8940..9089
                     /gene="rpmG"
                     /locus_tag="ACG8PN_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4861494.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9177..9695
                     /locus_tag="ACG8PN_001335"
     CDS             9177..9695
                     /locus_tag="ACG8PN_001335"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4861495.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9772..10728)
                     /locus_tag="ACG8PN_001336"
     CDS             complement(9772..10728)
                     /locus_tag="ACG8PN_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4861496.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10894..11700
                     /locus_tag="ACG8PN_001337"
     CDS             10894..11700
                     /locus_tag="ACG8PN_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861497.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11697..12686
                     /locus_tag="ACG8PN_001338"
     CDS             11697..12686
                     /locus_tag="ACG8PN_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4861498.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12683..13687
                     /locus_tag="ACG8PN_001339"
     CDS             12683..13687
                     /locus_tag="ACG8PN_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4861499.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13819..14361
                     /locus_tag="ACG8PN_001340"
     CDS             13819..14361
                     /locus_tag="ACG8PN_001340"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4861500.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14381..15079
                     /locus_tag="ACG8PN_001341"
     CDS             14381..15079
                     /locus_tag="ACG8PN_001341"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4861501.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15100..15312
                     /locus_tag="ACG8PN_001342"
     CDS             15100..15312
                     /locus_tag="ACG8PN_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4861502.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15312..16274
                     /locus_tag="ACG8PN_001343"
     CDS             15312..16274
                     /locus_tag="ACG8PN_001343"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4861503.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16292..16687
                     /locus_tag="ACG8PN_001344"
     CDS             16292..16687
                     /locus_tag="ACG8PN_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4861504.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16850..17215
                     /locus_tag="ACG8PN_001345"
     CDS             16850..17215
                     /locus_tag="ACG8PN_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4861505.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17212..19023
                     /locus_tag="ACG8PN_001346"
     CDS             17212..19023
                     /locus_tag="ACG8PN_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4861506.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19085..19252)
                     /locus_tag="ACG8PN_001347"
     CDS             complement(19085..19252)
                     /locus_tag="ACG8PN_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4861507.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6285 a         3303 c         3948 g         5892 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 gttccatggc atattagtat ccccctttat taaaaaacat aatctatata aacaagtgta
     8761 agggtattta tacaaaaaga aaaagatttt gtttgagaag taaaaccgtt gtatttatcg
     8821 tcaagtagtt ttacttaaca aaatcgtgaa aaacactagc aatctagaaa aaatcatggt
     8881 aagataatca agtctgagag tcatcttaga ggattcttgt aaaaatggga gggaaaaaca
     8941 tgcgcgtaaa catcacttta gagtgtactt cttgtaaaga acgtaactat ctaacaagca
     9001 aaaacaaacg taacaatcct gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca
     9061 aagttacttt gcaccgtgaa acaaaataat gtttttaaag ttctcaattc cttgctggtc
     9121 aaagaattga gacttttttt atctcaaaac atctaaaatc ataaggagcg gataatatgt
     9181 ttatttccat gtgggcacaa gataaaaatg gtctgatcgg caaaaatggt ttgttaccat
     9241 ggcgattgcc aaatgatatg cgtttctttc gcgaacatac aatggatcga attttagtga
     9301 tgggaagaaa gacctatgaa ggaatggggg atctttcact tccttatcgc catatcatcg
     9361 tgttgacaac gcaaccaaat tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg
     9421 atgaactttt gtcgtatagt caaacgatct cagaagatat ctatgtttcg ggtggtagtc
     9481 ggatttttca agaacttctt ccttacactg gtcggatctg gcgaacattg atcgatagca
     9541 cgtttgaagg cgatacgtat attgggaata ttgatttttc agacttcgcc cttatagagg
     9601 agtatgaggg gataacggat caagaaaatc tatatgccca tcgttttcaa aaatgggaaa
     9661 gagtaaaaaa tagggttgct tcaaaagaag aataaaatct atgcgtctag tttgttaata
     9721 agaaaaaaag catctcacga ctaatcgtga agattctttt tttagttaaa atcaatttag
     9781 tttttctttg atatcattga ccatttcctg actggcaata ggtccgttat atagccaact
     9841 agattcaggt ccgaattgca gtacatgatt gtctgtcact gctttaagat tcttccagct
     9901 tggttcattg aacatggcag catctttgtc actgttcaca aggatgatgt agtcagcatc
     9961 taattcagct agcttttcag aggaaacagc agaccagtca gaagtggctt ttttggagac
    10021 ctcttccact agatcaggaa caccaaattc cagatcgtcg tatacgattc ttccacttga
    10081 ccgatttttg ctgaccataa aggctgagtt gttggtcacc cacaacactg cagctgtctt
    10141 gttctcgatt ttatctgcta gttcttctcg agttttcttg atggattttt gataatcgga
    10201 aatgattttt tctgcctctt tttcttttgc caaagctttg ccgacatctt ttagttgttc
    10261 ttcccatgtg acatctgttc cgttttttac aacataagtt ggcgcgattt tgctatattg
    10321 ttcgtatttc ccgccttcga cagtagcgga agaagaaatc aacagcaaat caggctcaaa
    10381 gcttaacact ttctcataag gaaggtcata agaaatcgtt gggacatcct ttaaatcatc
    10441 ttgcagataa tcttgaatgg aaccactgcc aacagtccac tgggcgatag gtttttcccc
    10501 tagtgcaatc agatagtctt ctaagtaact gccgatgatc cgtttgggcg cgtttggaat
    10561 ctctacttta tgtccaagtg tatctgtgac tgtatgagta ctattggatg aagtcgtcga
    10621 attggcagtt gtagctgttt ctttatctga agagggggag caagcgctca gtactccagc
    10681 cagcagcaat atgctagata atgtgaatac ttttgttttt aatttcattt ctttcctcct
    10741 aacataattg agaatctttc tcattggtta ttattcctga tatttcgaac gctgtcaacc
    10801 gcttcatttg gctatagata tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa
    10861 ttgagaatga ttttcagcga aaggatgggt gaaatgacga gattaagagc agctgacatt
    10921 gcagccggct acgaaaacaa aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg
    10981 aaaatcacta gtttgattgg gccaaatgga agtggaaaat ctactttatt gaaaagtttt
    11041 acccgtatac tacctgtgaa atcaggaaag atcattgtca ataactctga tatctctaca
    11101 tacagttcaa aagaacttgc aaaaaaaatt acattattag cacaaactag tgaacaccct
    11161 acaggtatga cagtagaaga agtagtatct tacggacgat acccttatca aaaactgttc
    11221 agtggcatgg acacgcaaga tcatgaagca atcgagtggg cgttggatgc tacaggtcta
    11281 gctggatttc gtatgcggga tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg
    11341 atcgctatgg cgcttgctca ggaaacagat atcttgatct tggatgaacc gacaacattt
    11401 ttagatccgg cacaccaatt agagatccta cagttattga ctgaaatcaa tcaaatgaaa
    11461 caaacaacga tcttgatgtc gattcatgac attaatcatg cttcacgttt ttcagattat
    11521 ttattagcaa tgaaaaacgg taagttgatc acagaaggta cacctgaaga agtgatcaca
    11581 aaagagtgta tgaaaaaaat atttgagatt gatgcaacga ttgtcacgct tccaagaaca
    11641 aataaaccag tttttctaac ctatgatttg ttgccaaaag agcaggagga tcaattatga
    11701 ggactatgaa gaaaaataga ctcctgcttt tggtctgttt tgttcttttg attatttttt
    11761 ttgttgctct ttcaaatggc gcagcgactg tgggaaaagc agatttttgg caggcgttgt
    11821 ttgattttga tagatcaaaa caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg
    11881 tggcgggagc atttcttgta gggagttgtt ttgcattgag cggtgcattg atgcaaggag
    11941 tgacaagaaa tcctttagca gattcaggct tactaggaat caacgcgggt gcttcgttgg
    12001 caatggccct tagttttgct ttttggccta aagcttctga tttttctgtg tttctaatgt
    12061 cattggcagg ttcttttgct gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa
    12121 tgggcatgaa tcctttacag ctgattttag ctggcgtagc tattagttct ttttttactg
    12181 ctatcagcca agcacttacc caattcttta atctgcagca ggatcttgct ttttggtttg
    12241 taggcggagc tgccaatatc acatggaaac aattagcagt attgtttcct ttatatatac
    12301 ttgctgtttt ggtttctttt tcattaggaa aagcaatatc tttgattcat ttgagtgaaa
    12361 cacatgcatt agctcttgga agtcatccta aacgaacgcg gttgattgtt ttctgtctag
    12421 ttgcagtgtt agcagccatt gccgtttcgt tagtcggacc tgtttcattt attgggctgt
    12481 tggttccgcc agttgttcgc ggaatggttg gaacggacta tcgttatgtt ctacctgtct
    12541 cttttttagt tggcggtgga ttggttatgt tggcggattt tacagcgagg atgatcaatc
    12601 ccccttttga aacacctttt ggattagtca ttgcattgat tggcgttccg tttttactga
    12661 tacaagtaag gagggaacaa cggtgaaaaa aatatattgg acgttctatt tcttgctttt
    12721 tgtacttgtc atcctgtcta taggtagttt aatgattggt acgcctttta tttacctgga
    12781 tcagcttttt gccacgttca tagggaaaga atcatcttct ttggcgctga ttgttagaga
    12841 atttcgagtt cctcgattac tgatcagttt attagcagga gcttgtcttg gcgtttctgg
    12901 ttatatcttg caaggagtaa caagaaatga attggcagat tctagtatat tgggcattaa
    12961 tgccggagca ggatttttag tgatgcttta tttaggtttc ttttcgcact attcgctgcc
    13021 gtttctattg ctagttgtag cctttctagg tggtgtttta gctgcttgct gtgtctatct
    13081 tgcggcctat gatcgaaatg gctttttggg tatgaatcga atcttattgt ccggtattgc
    13141 cgttaatgcc ggcctttctg cattaacatt gctatgcacg atccaattat ctaaggaaaa
    13201 ttatggattt gtgaatgcat ggttagcggg atcaatctgg ggtgcgagct ggccttatgt
    13261 ttgggcattg cttccatggg cggttatctt aattccgcta ggaggacttt atgcacagag
    13321 gattggattg ctatcatttg gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa
    13381 aagtcaaaaa atccttttgt tgttagctgt tgctttggca agcggtagtg tagcggtgac
    13441 aggtagttta tcttttgtag gactactagc tcctcatgct gcgaagctgg tcgtacgtaa
    13501 agaaaataac tggacctttg ttctgagcgg tttgttcggt tccatttttc tgcttagtgc
    13561 agatatggtt gctagagtga ttctaccaag tggggaagta cctacaggaa ttttgattgc
    13621 cctttttggt gcgccatatt ttctgtatgc actttttatc agacaaaaac acgcgctgaa
    13681 cagttgaaat ttattaaaaa aggaaggcca atcatttcta ggtcttcttt ttattctatt
    13741 tatgcattta tttgaaagtt cgattgctat ttttcttttt ttcctatacc attttatgag
    13801 agaaggaagg gtgagttttt ggaccaattg aaagtaagat tacgccggaa aggaatcgaa
    13861 aagctgaaaa atctggccaa aagtggcaag aaaaaggaaa aagaacagcg aatctataaa
    13921 cagctattcg ccagcgagta ctggaagtca gcaaaagtga tagggacgac tttatcgaat
    13981 gaatttgaac tggatacaca gccggttatc caacgagcag aaaaagaaca aaagaaaata
    14041 gttgtaccta aaactttgcc gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc
    14101 gttcttgtac gttcgagttt tggtgttctt gagccacaat cagagaagct ctatttgccg
    14161 gagatgatcg atctcctcat tgtaccaggt attatctaca atagagccgg ttatcgaatc
    14221 ggttttggcg gtggttatta cgaccggtat ttaaataatt ttcataaaaa aacatgtagc
    14281 cttgtttttc gtgaacaatt gaatgaacaa tggcaaccgg agagctttga tcagaagatc
    14341 caacaattgt ttattgacta aaatgaaatg ggaggttttc atgaattatc aacagcaaat
    14401 aaaattgcgt gcatggttaa gacgaccgtt atggacctat gcttttttag ggatacagac
    14461 agcggtcttc ctcatgatgg aattatttcc gcgacttgag atcccttatt atgccggaat
    14521 gtatggtcct tatcttgttc acttcaatga atggtggcgt ttagtgacac caatcttcat
    14581 ccattttgga ctgatgcatt tcgtaatgaa ctcactgata ctgtatttta tggggcagca
    14641 aatcgaggca atctatggcc attggcgttt tttcttgatt tatatgttca gcgggataat
    14701 gggaaataca gcaagctttg cttttaacga agcgaatgtt ctgtctggtg gtgcgagtac
    14761 ttcgatattc ggtttgttcg gtgcgttgtt tatcttaggg tttcatttta aacataaccc
    14821 agcgatccag cagctagtca gacattttct tttatttatt gtgttgacat ttgtttttgg
    14881 tcttctggat acctctgtag atgtttgggg acatgtcggt gggctaattg gcggattagt
    14941 gttagggaat gttttgggtc taccgaaaca acagacaagc tattcgatac accaaaggat
    15001 tctttctaca ctagtttttg tatttctttt tgtaatatgt attttactgg gtttaaaaaa
    15061 atatggttta cttgtataat atgaaggaag tgcttaaaga tggagagctt atatgatgta
    15121 cagcagctgt tcaaaaaatt tggcatcttt atctatgtgg gtgcacgcat ctatgacatt
    15181 gaattaatga ctatcgaatt gcgtaatctg tatgatagcc gtttgattga tcatgataca
    15241 tacatgacag cgtggcgcat attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa
    15301 ggaaatttgt aatggacaag aagattattg ggattgattt aggtggaaca acagcaaaat
    15361 ttgcaatttt aacaccagaa ggagagattc aacaaaaatg gagcatcgat accaatattt
    15421 tggatgatgg taagcatatc gttcctgaaa tcattgaatc aatcaaccat cgtttgaatt
    15481 tatataatat gaaagccgaa gactttatcg gaatcggcat gggtacccca ggaagcgtgg
    15541 acagcgaagc tggaacggtt attggtgcgt ataacttaaa ctggacagaa gttcaatttg
    15601 tgaaaaaact gatcgaggca ggaacaggaa tcaaattcgc tatcgataat gatgcgaatg
    15661 ttgctgcttt aggtgaacga tggaaaggtg ctggagaaaa cgatccagac gttgtattcg
    15721 ttactctagg aacaggtgtt ggcggtggta tcgttgctgg cggaaatttg attcacggcg
    15781 tgagcggagc aggcggagag atcggccata tcactgtgga tcctgaaggg ttcgaatgta
    15841 cttgcggtaa acgcggatgt ttagaaactg tgtctagcgc tactggtgtg gtacgtctag
    15901 gtcgttactt ggcagaagaa tacgcaggag attcaaaact gaaagcaatg ttagacaacg
    15961 gtgaagaagt aacaagtaaa gatatctttg aaatggctca agaagacgat ccgtttgcat
    16021 tgatggttgt tgatcgcgta tgcttctact taggtttagc ttgcgccaac ttggggaata
    16081 cattgaatcc gtcaagtatt gtgttaggtg gaggagtatc ggcagcagga gaatttttac
    16141 gtagccgtgt agagaaatat tttaaagaat ttactttccc gcaagtaaga gaatcaacaa
    16201 aaatcaaatt agcagagctt ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct
    16261 tgaaatttgc ttaaaaaaac ggaggcatta catggtttta tgggtaatta atatcatttt
    16321 gctattgatt gtgcttgcaa tcatcttttg ggaagtctat ctacgagtaa tggccaagcg
    16381 ttcagcaacg actttgacag aagaagaatt tcgagaagga atgagaaaag cacaagtaat
    16441 cgatgttcgc gaaaaagatg tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc
    16501 ttacactgtt ttgaaagatt cattaggatc tattcgaaaa gaccagcctg tctatattta
    16561 cgatcaaaag aaaagcttga gcatccgtac agcaaataaa ttacgtaaag caggttatca
    16621 agagatttat ttacttaagg gtggatatga cggatggtct ggaaaaatca aaagtaaaaa
    16681 agtataaaac agatgattgt atgacagctt taaaaaccga atcaatcatt agattttgac
    16741 caagatcaca tgaacggcgc aatggatagt tgtgccgttc atttttttta ttttgtgcta
    16801 ttatctacat atataagcta agcgaaaagg cagaaagaag gaaacaacta tgatcaaatt
    16861 aaaacgtgtt taccttgatg ctgaaaaaga ggatggctat agaatactcg tggataggtt
    16921 ctggccacga ggaatttcaa aagaaaaagc gcagttagat ctgtggttga aagaaattgc
    16981 acctagtaat gaagcacgca aaacttttga ccatgtacca gaacgatttg cttcatttgc
    17041 aaagaaatac cagcaagaat tgattttcga agaagaaaag aagcaagcta tttttaaact
    17101 cagtcaactt atgaaagaac atccagtcat tacttttgtt tatggtgcaa aaaatgaaca
    17161 tcagaaccat gctgttttat tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata
    17221 gggatcattg gtgccggacc tagaggatta agtatagcag aaaggattct ccataataat
    17281 caggacagac attctcttga actttttctt ttcgatccca atggtcctgg aggtcgagtt
    17341 tggcggttga atcagccacc ggagttatta atgaattctg tttctcaaca agtgacgctt
    17401 tttacagatg aaacattgtc cagtggagga ttggtaacac agggacccaa cttgtttcaa
    17461 tggagtaaag gacctgctac agattatatc gaacagacgg taaccaaaaa taagcaacta
    17521 attatagaag aaatcaatcg tctggaaaaa gatcagcaat ctactcgatg tttatacgga
    17581 ctctatcaac gctggttttt ctcaaagttg caagaagaat ttccagcttc tattcagttt
    17641 gtttattctt tagtaaaaaa agtcagcaaa agaaaagatg gttatctttt acagacagat
    17701 cagcaaagct attctatcga tcagcttgtt ttagcaagcg gtcactggga aaatgatgag
    17761 acagaagaag aaagagtgtg ggtagactac gcaaagaaaa atgatttact gtatcagctt
    17821 ccttctaatc ctgcagatgt cgatctttcg aagataccgc ctaaagaaaa tgtcttttta
    17881 agaggtctag gcttaagttt ttttgattat gtcggattgt ttacagtatc aagagaaggg
    17941 cgcttcgaga acgaaaaagg aaaacttgtc tatcatccat caggcaagga accgattgta
    18001 tacagtggat caagaagggg gctcccttat taccctcgag gaagaaacca aaagcaaggc
    18061 ggatcaatgg catggccacg gttactaaca aaagaaaacc tggagcaatg gcatcagtcg
    18121 ggatttttga ctggcgaaac attttttgac tatctgaaaa aagacgttga gttgttttat
    18181 tacaagaaaa tcatcgaaga atatcatttg ccgattccaa agaaaaattt tgagcatgat
    18241 tttctctccc tctcttcaga ggagtgtctc ggaaagcatc cagcactaat cccttataga
    18301 tgggattggt cgtatttaga aattcctctt acctatcagg atgagtcctt tgcagaagct
    18361 agccgagcat ttattgaagg gcagataaaa gaagcggaaa aaggcaattg cacaggagca
    18421 tttgcttcaa catttgatgc attaaaagac tggcgtgatc caatccggca ggctattgaa
    18481 tggcacgttt tttctgcaaa ggaatacaaa gaaatactat ggggatggtt tactcatcta
    18541 aatagctttt taacgatcgg tccgccagtc attcggacga aagaattagc agcactgata
    18601 gatgcgggta tttttcattt gttcgaaccg ccatttgata ttcagcaaaa gcacaaccgc
    18661 tatgttattt ctctacaaga taaagaaatc agcagtcgct atttgattga agctcgattg
    18721 ccacaaacaa gcttttctcg ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa
    18781 cttgttcgcc ctttttcatt ttcagataat acaggaagtt atcattcaga agctatagat
    18841 gttgagcgag gtacgaatcg cgtgattgat gcttcagggg aaatccagtc tgacttttat
    18901 tgctatggaa tacctgttga aggtgtggat tggctaactg cagcagtagc tagaccttat
    18961 acagatccat ggaatctgag acaggcagat aaaatagcac aacttatatt gactgacaaa
    19021 taaaaatacg catgatgact tatctgttct ttcacaagtc ttcatgcgtg tttttaaata
    19081 attattaacg agaaacgcgt ttgcgcattg cttttttacg gttttcttcg atcattgctt
    19141 ctttttcgtc aattggatcg atcactgttt ttttcacaga agcgaccaaa ccagcaacgg
    19201 cagcggctgt tgccagactt cctactaata ttccagatac aaattttttc attgaatcca
    19261 cctcatttat tatttgttat atctattatg gaacaaaaga gaaaaaaagg ctagttttac
    19321 gtttgtttgt aaacctttgc aaaaaatcct tgaaaatgtt tactaaatgt tttattataa
    19381 atttaccata tgaatttacg aaattttact ttgtttgttt tcaccata
//
LOCUS       DAZUKA010000049        19068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000049 DAZUKA010000000
VERSION     DAZUKA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.049"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..835
                     /locus_tag="ACG8PN_001348"
     CDS             83..835
                     /locus_tag="ACG8PN_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4861508.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            832..1746
                     /gene="pfkB"
                     /locus_tag="ACG8PN_001349"
     CDS             832..1746
                     /gene="pfkB"
                     /locus_tag="ACG8PN_001349"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4861509.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1764..3695
                     /locus_tag="ACG8PN_001350"
     CDS             1764..3695
                     /locus_tag="ACG8PN_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4861510.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3806..5365)
                     /locus_tag="ACG8PN_001351"
     CDS             complement(3806..5365)
                     /locus_tag="ACG8PN_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4861511.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5520..7754
                     /gene="pcrA"
                     /locus_tag="ACG8PN_001352"
     CDS             5520..7754
                     /gene="pcrA"
                     /locus_tag="ACG8PN_001352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4861512.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7771..9807
                     /gene="ligA"
                     /locus_tag="ACG8PN_001353"
     CDS             7771..9807
                     /gene="ligA"
                     /locus_tag="ACG8PN_001353"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4861513.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9913..10218
                     /gene="gatC"
                     /locus_tag="ACG8PN_001354"
     CDS             9913..10218
                     /gene="gatC"
                     /locus_tag="ACG8PN_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4861514.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10218..11690
                     /gene="gatA"
                     /locus_tag="ACG8PN_001355"
     CDS             10218..11690
                     /gene="gatA"
                     /locus_tag="ACG8PN_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4861515.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11687..13117
                     /gene="gatB"
                     /locus_tag="ACG8PN_001356"
     CDS             11687..13117
                     /gene="gatB"
                     /locus_tag="ACG8PN_001356"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4861516.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13127..14182
                     /locus_tag="ACG8PN_001357"
     CDS             13127..14182
                     /locus_tag="ACG8PN_001357"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4861517.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14293..15669
                     /gene="rlmD"
                     /locus_tag="ACG8PN_001358"
     CDS             14293..15669
                     /gene="rlmD"
                     /locus_tag="ACG8PN_001358"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4861518.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15878..16714
                     /locus_tag="ACG8PN_001359"
     CDS             15878..16714
                     /locus_tag="ACG8PN_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4861519.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16863..17084
                     /locus_tag="ACG8PN_001360"
     CDS             16863..17084
                     /locus_tag="ACG8PN_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861520.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17085..>19068
                     /locus_tag="ACG8PN_001361"
     CDS             17085..>19068
                     /locus_tag="ACG8PN_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFS4861521.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLI"
BASE COUNT         6433 a         3356 c         4158 g         5121 t
ORIGIN      
        1 aatttttatg aacgtttttg actgatttat attgcgagta gaaaaaaaat atgctattat
       61 ttgtattgag gtgatcgtga aaatgcttac agaggaacgt agacaaaaaa tcctagactt
      121 gcttgaacag cagaaaatcg tgaaatcaca agatcttgta aaccttttta atgcttctga
      181 atcaacgata agacgtgatt tacaagagct ggagaatagt gggtttctag aaagagttca
      241 tggcggagct aagaaagagc aattattgaa ttttgagcag aatatgagtg aaaaatcact
      301 caaaaacgtt cacgaaaaac agcaaatcgc caaatcggca gctgaaacca ttaatgacga
      361 agatgtcatt tatttagacg ctggctctac tacgttagaa atgattcctt ttctgcaagg
      421 aaaacagatc acagtagtga caaattccgt tcaacatgcg gcaaatttag ttgatttgga
      481 gcttgctacc attattttag gtggaacgat aaagctggca actaatgctg ttttaggatc
      541 taatgcgata gaacaactaa aaaattttca ttttaataaa gcatttatgg gaatgaacgg
      601 tgcccatctt gaacaaggat tcacgactcc ggacccagaa gaggcagctg ttaaacgttt
      661 agccatgaaa aattccgaag agacatttgt attgattgat cataccaaat tcaaccagtt
      721 gtccttcacc tctgttgcac ctttggaagc agccacgatc ataaccgagc gttgtccatt
      781 agaattcgtt aatgaattcc gtgaaaagac aacaattaag gaggcaatac aatgatttac
      841 acagtaacac ttaatccatc aattgattat atcgtacgcg tagatgggtt aaaactaggc
      901 gatttgaacc gaatgaagga agacttcaag ttacctggag gaaaagggat caatgtttcc
      961 cgtattttga aacggatcga ttctcaatct acagcactag ggtttttagg agggttcact
     1021 ggcgaattta tttcgaagtg gcttgaaaaa gaacaaatcc agtctttgtt cacaaccgta
     1081 aaagaagata caagaatcaa tatcaaacta aaatcagata ctgaaactga aattaacggt
     1141 ttgggcccat ctatttcaga tgaagaaatc gatgagttga agcaagcatt aggaacagta
     1201 ggacaaggag atatcgtcgt attgtcagga agcgcgccgg ctagcctgcg aaaaggattt
     1261 tatcaagaac tgattgatat cattcgagaa aaaggtgcag atttcgtgat tgatacaaca
     1321 ggtgatgacc taaaaaatgc acttgttaag aaaccattgt tgatcaaacc aaacaatcac
     1381 gaattagctg agctttacaa tacaaccttc caatctgtag aagaaatcat tccgtatggt
     1441 aaaaaattgc tagctgaagg ggcacagaat gtgctgatct caatggctgg agacggtgct
     1501 ttgctcttca cggaacaagg aacttataaa acgaatgtat tagttcgccc attgaaaaat
     1561 tcagtaggtg caggggattc aatgattgcc ggctttattg gggcttacag ccgtacgaaa
     1621 gatccaatcg aagcctttaa atggggagtt gcttgcggaa gcgcaacagc tttttctgat
     1681 gacttggcag ctcgcgaatt gattgatgag ttgctgccag aagttgaaat cacaaaaatt
     1741 gattagtttc aaggaggata atcatgaata tcaaggattt aatggtcaaa gaagcgatga
     1801 tcatggattt gcaagcgact gataaaaaag gcgcaatcga tgaaatggtc caaaaaatgt
     1861 ttgatgccgg ccggatttcc gatatcgaaa catataaaga aggaattcta gcccgcgaag
     1921 cacaaacttc tacaggtctt ggtgatggaa tcgccatgcc tcacgcgaaa aacagtgcag
     1981 ttaaagaagc aacagtttta ttcgcaaaaa gccaaaaagg agtagattat gaagccttag
     2041 acggacagcc aacttatctg tttttcatga tcgcagctcc agaaggcgca aacgacacac
     2101 atttgcaagc attagcagca ctatctcgat tgctgattga tgcagatttc gtaggaaaat
     2161 taaaagaagc aaatacacca gaagaagtcc aagacttgtt ccaaacagct gaagagcaaa
     2221 aagaagcaga agaaaaagca gaacaagaaa cacaagctgc agaagcttct gctcatccag
     2281 aaagaaaatt cgttgttgcc gttacagctt gtccaacagg gatcgctcac acttacatgg
     2341 cagaagatgc tctgaagaaa aaagcgaaag aaatgggtgt ggagatcaaa gtcgaaacga
     2401 acggttcaga aggaatcaaa aaccgattga ctgctgaaga catcgcccgt gcagaaggtg
     2461 taatcgttgc agcagacaaa aaagtagaga tgaatcgctt tgacggaaaa gaactgatca
     2521 atcgtcctgt cagtgatggt atccgtaaac cagaagagct gatcaatcta gcccttagtg
     2581 gtactgcacc agtattccat ggtgatggaa aagaaagcag ttcagaagaa ggaaatgcgg
     2641 atggtactat cggacaaaga atttacaaag atttgatgaa cggcgtatca cacatgttac
     2701 ctttcgttat tggtggagga atcgctattg cgctttcttt catgatcgac caatttattg
     2761 gcgtgccaca tgatcaatta tcaaatctag ggaattacaa tcaagctgct agctggttca
     2821 accaaatcgg acaagctgca tttggtttca tgctgccggt attagcagga tttattgctt
     2881 caagtatcgg tgaccgccct ggtttgatcg ttggatttgc tgctggtgca ttagctaata
     2941 caggcggtgc aggattctta ggtgcactga ttggtggatt cttagctggt tatgtcatcg
     3001 tatttttacg taaattattc aagaacttac caaaatcatt ggatggaatt aaaacgatcc
     3061 tcttttatcc agtgtttggc ttactgatta ctggtttctt gatgttgctg gtcaatgtcc
     3121 ctatgaaagc aatcaatgat gcattgaacg gtttcttgac aagtatgagt ggttcgaatg
     3181 ctgctttact tggtgcatta ttagctggaa tgatggcggc agatttaggc ggtccaatca
     3241 acaaagccgc ttatgtgttt ggtacagcaa ctttagctac gactgttgca actggcggaa
     3301 gtgtcgtgat ggcttctgtt atggctggag gaatggtacc tccattggca attttcgttg
     3361 ctacacgttt gtttaagaat aaattcagca aaacagatca agatgctggt ttgacgaaca
     3421 tcgtgatggg actatcgttt gtcacggaag gatcgattcc atttgcagca gctgacccga
     3481 ttcgtgccat tccaagtttt attattggtt ctgctttaac tggcggatta gtcggagctt
     3541 tcgggatcaa attactggct cctcatggcg ggattttcgt tgtattctta ctgagtcacc
     3601 cggtcatgta tttggtgttt attgctatcg gagcagtcgt atctggtgtg atttatggtt
     3661 tattgagaaa agctcctgca gcagcagaag catagaaata aaaaagaaga ggctggtttt
     3721 tcccagtctc ttcttttttt atagaatcat atagaatcaa gtagtgtaga tgaatagttg
     3781 ttgtaaaata gtttttccat gttcgttaaa atgattgctt caaatttgtg atagaagatt
     3841 tagcctcgat ttcctgcttg attcttggca agtttgcttc atcagtcaag ataaccaaaa
     3901 tatctcctac attcatgatc gtatcaccgt gagtcaagat ttcctgattt cctctgcgta
     3961 tagagattag cagcatattt ttaggccaat tgaagtctcg taccattaat ccgtctaaag
     4021 aactctctgc cgttactgga aaatcaaaag ttgtccttgc accttttagt gaggcggtct
     4081 gagtatttcc aagtagacgt tccaataatg attcgtaaat tggattaccg ccgagaaggt
     4141 cattgacgat ataagcaaca agagcagcca atcctaaagg catcagatga ttaagagagc
     4201 caaccatttc tgtcactaaa ataattgctg tcagcggtgc ttttccaatc gctgtgaaat
     4261 aaccagccat ggcaataacg acaaaatcct tgatgtagat tggatccatc ccgaaatcaa
     4321 ccataatgtt tccataaaag ctaccgatta aagcccccaa tgtcaatatc ggaaggaaaa
     4381 tcccccctgg aagaccacca ccgtaagaaa tcatggaaaa aacgaatcga agaacaaaca
     4441 aaccgataat cataatgatt ggcaaggaag catgtccaaa agctaaaacg atttgattgc
     4501 ctccgcctag agtactaggc cataaataac caattggcaa aatcagcaag aaaggaacga
     4561 acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa ttttggcaaa gccagcaaga
     4621 ccttctgata aataaaacct aagatgccta gaagtactcc taagatcact aaggaaccat
     4681 aatatttcaa cggcaaagaa ggaaggtcac ctagatataa aacaggtctc aagccgaaaa
     4741 agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa tgatgtcagc caaacaagtg
     4801 gagaaaagga gtgatggatt tcttctaaaa caaagagtaa tcctgcgata ggagcattga
     4861 atgctgctgc aagtccgcta cttgcaccgc tagaaattaa aatcttttct tctgaagatg
     4921 gactccgaaa caattgtcca gctccttgtc caatggatgc gccaagctgt atagaaggtc
     4981 cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac accgccaatg aactttttcc
     5041 ataaaacaga gaaccagttc attgataatt ccccgcgaag ctgtccctct acttgtggaa
     5101 tcccgcttcc cttgatatcc ggttcatttt tgaccagata acccacgaaa aatgctgcaa
     5161 ggatgcagca aatagctagg ccaattaagt aaatcggctg ttcccgactc atttcataga
     5221 tacttcgaac taaatcgctt agcttttcga tagataatcg aaacaggcta acgacgattc
     5281 ctgtcagcag tccaacaaat attcctttta gaataaaata tatctgagtc ttatctaatt
     5341 tcttgatttt tccttttttt tccattcaaa cgcctcgctt tcttttaatt tagcgcgaaa
     5401 tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa tgattttgaa ctagctagtt
     5461 taatgaaagc caagaaaatg ctataattaa caagattgat aaaagaaagg atgttgttaa
     5521 tgtcatccaa agctgaatta ttaaacggga tgaacccccg tcagaaagaa gctgttcttc
     5581 acactgacgg accattactg ctaatggcag gtgcaggaag tggaaagacg agagtgctga
     5641 cacaccgcat tgcctatttg attgaggaaa aagaagtcaa tccttggaac atcctagcga
     5701 ttacttttac caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg attctcgcat
     5761 ctggaggcga agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt attttaagaa
     5821 gagatgttga ttttatcggg tataaccgga actttacaat catcgattct tctgaacagc
     5881 tgaccttgat gaaacggata ctaaaagaac tgaacattga tccgaaaaaa tatgatcctc
     5941 gaagtatatt aggaacaatc tctcaagcaa aaaacagcct gcagacccct caggattttg
     6001 caaaaatgca aggcagttat tacgaagaga ttgcggcaaa atgttatgct gcttaccaaa
     6061 aagagcttca atataatcaa tgtatggact ttgacgacct gatcatgaat acgattcgat
     6121 tatttgaaga acatcctgat tcactcacat attatcaaaa caaattccat tatattcatg
     6181 tagatgagta ccaagataca aatcacgcac agtatacgct agtcaattta ttggcaggta
     6241 gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag tatttacggc tggcgcggtg
     6301 cagatatgca gaacatcttg gattttgaaa aagattatcc agatgctgca gtaattttgc
     6361 tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa gtcattgaaa
     6421 acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga aacaagatca
     6481 cttactaccg tgcagataat gaacgagacg aaacccgttt tatcgtagat cggatgcaag
     6541 aagagattcg aagcaatcat cggaattacg gcgactttgc catcttgtac cggacgaacg
     6601 cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa tattccttat aaaatggttg
     6661 gagggcacaa gttctacgat cgtaaagaaa tcaaagatat tttagcgtat ttgaatgtgc
     6721 tggcaaatcc tcaagattca atcagttttg agcgtatcgt taattcgccc aaacgaggca
     6781 tcggaccagg atctatcgaa aaactacgca gttttgcttc tcttcatgaa tggccactgc
     6841 ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga cagcaattag
     6901 gtgcatttgg agagatgatc caagaagtca ctcagatgat cccatacttg acagtgacag
     6961 aattgacgaa agaggttttg gatcgtagcg gatatctaga agacttaaaa atccaaaata
     7021 cattagaagc acaagcacgc atcgaaaact tggaggaatt tttaacggtc acacaagaat
     7081 tcgacaaaca attcgaacag caaaatgaag aagacgctga tgcaccagaa gaaaaactaa
     7141 cggtcttctt aaatgatctg gcacttgttt ccgatattga caatttagaa gaagatgctt
     7201 cccaagtgac attgatgact ttgcatgcag ccaaaggatt agaattccca gttgtattcc
     7261 tgattggtct agaagagggg gtttttcctt tatctcgagc attgatggaa gaaagcgaac
     7321 tggaagaaga acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa gcgttgtatt
     7381 taacaaatgc tttctccaga acattatatg gacggacgca atataaccga ccttctcgtt
     7441 ttgtagaaga aatcgatcag gagttactgg aaatcgaagg catgcgtcct acgcctaaaa
     7501 aaacacctgt attcgctaaa aaaacagcat atagctataa acagccagag acagcggttg
     7561 ttccctctaa aagtgctact ggaggggaaa ataacagctg gaaacctgga gataaagtca
     7621 aacacaaaaa atggggtcta ggaacagtcg tccgagtaag cgggacgtcg aaggatctag
     7681 aactagatgt ggctttccca agtcaaggag taaaacgtct tttggctgcc tttgcgccaa
     7741 ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg aaatgacgat tgacgaagct
     7801 gctaaacgag cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga atactacgta
     7861 gaagataaac caacagtgga agactatgtt tatgataaag aatacgcaga attagtagca
     7921 attgaggaac aatatcctga tttgatcacg tctgattctc ccactcaacg agtaggcgga
     7981 aaagtattag aaggttttga aaaagtcact catgatattc cgctttacag tttgaacgac
     8041 gttttcagca aagaagagct gatagctttt gatcaacgtg tacaaaaagc agtgggacgt
     8101 gtagttgact attgctgtga actaaaaata gatggacttt ccgtttcttt gcgttatgaa
     8161 gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga aaacatcact
     8221 gaaaacctaa aaactgtccg gtcggtccca atcaaattaa aagaaccaat gaacatcgaa
     8281 gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc aattgaacca agacagagaa
     8341 gcagagggaa aagatatctt tgcgaatcct cggaacgccg cagctggttc tttgcgtcag
     8401 ttagattcta aaatcacagc gaaaagaaat ctggacactt ttttatacac agtagctgat
     8461 ttcggtccta tgcaagcaaa aacccagtat gatgcgctag aagagctaga aaaaattgga
     8521 ttccacacga atcgggaaaa acgtctatgt cattcgattg atgaagtgtg gtcatacatt
     8581 gaagaatatc atgacaaacg tgtggatttg ccctatgaaa tcgatggaat cgtcatcaaa
     8641 gtgaatgaat tctcgctaca agatcaactt ggttttactg tcaaagcacc gagatgggca
     8701 gcagcttaca agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat cgaatggaca
     8761 gtcggtcgaa caggggtagt aacgccaaca gctatcatga caccagtccg agtagcagga
     8821 acgacagtaa gccgtgccag cctgcataac ggtgattata ttaagttgaa ggatatccgc
     8881 ttgaaagata cagttctgat ttataaagcg ggagacatca tcccagaagt ttcgcaagtc
     8941 gtgttagata aacgacctaa agacagcgaa gaataccaat tgcctacaca ttgtccagtt
     9001 tgtggaagtg aacttgtcca tttggatgaa gaagtagcct tacggtgcat caatcctaaa
     9061 tgccctgctc aaatgaaaga aggtttgaac cactttgttt caagaaatgc gatgaatatc
     9121 gatgggttgg ggccgcgcgt actagaacag atgtatgata aaaaactagt agcagatgtt
     9181 gctgatcttt acaaattgac agaagaagag ttattgacgt tggacaaaat aaaagaaaaa
     9241 tcagcaaata atattttgac agcaatcgac aacagcaaag ataattcggt cgagcggttg
     9301 attttcgggt taggaatccg acatgtcgga gcgaaagcag caaaaatttt agcagaacat
     9361 ttcggtgatc tagaaacatt gagtaaaagt gactatgagt cgattattgc gcttgatacg
     9421 atcggtgata tcattgccga cagtgtagtc acttatttca gtaacgaaga agtccatgaa
     9481 ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat acaaagggct tcgaaatgcc
     9541 caattgcaag aagtcgagtc tccttttaaa gaaaaaacag tagtccttac tggtaaacta
     9601 acacgattta ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg gaaagtgact
     9661 ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg cagcaagctg
     9721 accaaagcac aagaattagg aatcgaagtt tggacagaag atcaaatggc agatgcattg
     9781 gctaaaagtc gctcagtaga agaatgaaaa atgagttttt tcagacaaat ttcaataaac
     9841 aatttcagac acggcactct tgtgttaaaa tagagagcaa agtttttaac tcgtttagaa
     9901 agaaggggta gtatggcgat tagtgaagaa caagtgaaac acgttgccaa attagcaaaa
     9961 cttgcgttcg cagatgaaga acttgcaagt tttaccgacc aattaggtaa aatcatcgat
    10021 atggttgaaa tgctggaaga agtagataca gaaggtgttc catttacgtc gaatgtggtt
    10081 gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg cgatgaatta
    10141 ttgaaaaatg tgccggaaac agaagacggc tttatcaaag tacccgcaat catcgataat
    10201 ggggaggctg gcgcataatg acagaattac acaatcgttc tctagaagaa ctgcatcata
    10261 tgcttgtatc gaaagaaatc actgtacaag atttgacaaa agcaacattt gaacgaatca
    10321 aagaaacaga gcctgaggtc gaagcattca ttacattgaa cgaagaaaaa gcattggaac
    10381 aggcaaaagc tttagatgaa aaagggattg atgaaagcaa cgtccttgct ggtattccaa
    10441 ttggtatcaa ggataatatc gtaacaaaag atatcttgac aaccgctgca tctaagatcc
    10501 tttacaattt tgaaccaatt tatgatgcaa cggtcatgga taaaatctat caagcagata
    10561 tgatccctgt tggtaagcta aacatggatg aatttgctat gggtggaagt acagaaactt
    10621 cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa agtacctggt ggctcttctg
    10681 gtggttctgc agcagcagtt gcagctggac aaattccagt ttctttaggt agtgatactg
    10741 ggggaagtat ccgccaacca gcagctttta acggcatcgt agggatgaaa cctacttatg
    10801 gccgtgtctc acgttttggt ttgattgcct ttgcttcatc tcttgaccaa atcggaccaa
    10861 tgacccgtac ggtgaaagat aatgcattag cattgacagc tatcagcggt tatgacgaaa
    10921 aagacggtac ttcttcaggc gtttcagttc ctgattttgc agagggactg acaggcgaca
    10981 tcaaaggaat gaagattgct ttacctaaag aatacttagg cgaaggtgtg gcccctggcg
    11041 taaaagaagc tgtattgaaa gcagcagaaa cattccgttc attaggtgct gttgtggaag
    11101 aagtcagctt gcctcattca aaatacggtg tggctgttta ttatatcatt gcctcatcag
    11161 aagcaagttc caacttgcaa cgattcgatg gtatccgcta cggttatcgc tcagaaaatg
    11221 tgaaatcatt agatgatgta tatgtcaact cacgttcaga aggattcggt gaagaagtaa
    11281 aacgccgtat catgttaggt acattctcct taagtgctgg ttattatgac gcttacttca
    11341 aaaaagcggg acaagtccgt acattgatca aacgagattt tgaaaaagtc tttgcagatt
    11401 atgacttgat catcgggcca tcttcaccaa cagttgcatt cggaatcggt gagaacatta
    11461 acgatccaat cacaatgtac atgagtgaca tcttaacgat ccctgtcaac ttggctggac
    11521 ttccaggtat gtctgtaccg gcaggtttat cagaaggatt accagttgga ttgcagatca
    11581 tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt gaacaagcaa
    11641 cagaattcca taaacaacaa ccagctattt taggagggaa agacgaatga attttgaaac
    11701 agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt tctctccagc
    11761 gccagctcat tttggtgcag aaccaaacag caatacaaac gtgatcgact ggggttatcc
    11821 aggcgtgctg ccagtaatga ataaagctgc gctggaattc gggatgaaag cagctttggc
    11881 attaaactgt gaaatctcta aagatactca ttttgaccgg aaaaattact tttacccaga
    11941 taatccaaaa gcatatcaaa tttcccaatt tgatcaacca atcggtcatg acggatggat
    12001 cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc atttagaaga
    12061 agatgctgga aaaaacatgc atggtattgg cggttattct tacgttgatt tgaaccgaca
    12121 aggcacgcct ttgatcgaaa tcgtttctga agctgatatg cgttcaccag aagaagcata
    12181 cgcttatcta gaagcattgc gatcaattat ccaatttaca gaagtcagcg atgtgaaaat
    12241 ggaagaaggc tctatgcgct gtgatgccaa tatttctctt cgcccttatg gacaagaaga
    12301 gttcggtacg aaagcagaat tgaaaaactt gaactcttta tctttcgtga aaaaaggttt
    12361 agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa tccaacaaga
    12421 aacacgccgt tttgacgaaa caacgaataa aacagttttg atgcgtgtca aagaagggtc
    12481 tagtgattat cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg atgacgaatg
    12541 gattcaaaaa gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg aacgctatat
    12601 ccgtgagttt ggacttccag agtatgacgc aatggtattg acacttacaa aagaaatgtc
    12661 tgattttttt gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat ctaactggtt
    12721 gatgggtgaa gtttctgctt acttgaacag tgagaaactg gaattagctg agacaaaatt
    12781 gacacctgag aacttagcag gaatgattcg tttgatcgcc gatggaacga tcagctcaaa
    12841 aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc ggagaagccc aagaagtagt
    12901 tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa ctactgccaa tcattaatga
    12961 aattttggat aacaaccaac aatctgtcga tgatttcaaa aatggaaaag atcgtgcggt
    13021 tggcttccta gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc ctggtgtcgt
    13081 gaataagcta cttaaagatg aattagcgaa acgatagagg ctgaaaatga aaaaagcacg
    13141 cgtaatttat aaccccacct cgggaaaaga attattgaaa aaaaatttgg cggatatcct
    13201 acaggcatta gaagaagcag gatttgaagc cagcgcctat gcaactacac cagaaccaga
    13261 ttcagccaag aatgaagcat gtagagtcgc agaactaggt tttgatctag tagtggcagc
    13321 tggtggcgat ggcacaatca acgaagtggt taatggaatc gctccgcttg aacaccgtcc
    13381 taaaatggcc atcatcccag ctggtacaac gaacgactat gctcgagcat taaaaattcc
    13441 tagagataat attaaagcag cagcagaagt gataaaaaaa caccaaaccg taaaaatgga
    13501 catcggcctt tctgataaca gttacttcat caatatcgct gcaggtggct atttgacaga
    13561 attgacttat gaagttccat cagaactaaa gagtattttt ggttatcttg cttatcttgc
    13621 gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga agtatgatga
    13681 aggggaatat atcggaaatg cttcgatgtt tttcctaggg ctgacgaact ctgttggagg
    13741 attcgaacag atcgcgccag atgccaaatt ggatgatggg aagttttctt taatcattgt
    13801 gaagacagca aatatctttg agatcttgca tattgcagca ctcatgctga atggcggtcg
    13861 acatgtcgat gatccaagaa taatctatac caaaacaagc agtttgttcg ttgaaacaga
    13921 agatgatatc aatcgaccag taatgatcaa cttagatggt gaatacggcg gggaagcacc
    13981 gatgcatttc caaaatcttc atcaacacat tgagttcttt gccaataccg atcagattcc
    14041 tgatgaagca atcacaggta cagatgaaga agaattagag gttgtcagca aagaattcgt
    14101 aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa tagcagacga
    14161 aacacctaat caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg tcagtgccgg
    14221 ttttagttgt gtaattagct gatttatagt agagttcagt aggattgaaa aataggctta
    14281 aaggagcaaa atatgacaga acagcaaata gtaaagaaaa acgaacgata tacagtcgaa
    14341 atcgtcgact tgagttatga aggaatgggg gtagcaaaaa tcgatggtta tccattattt
    14401 atcgaaaatg cactccctgg agaagaagta gaaatactag tagtaaaagc aggcaataag
    14461 tttggatatg gaaaagtcga aacatttatt acacaatcac ctgatcgtca gcctgtagaa
    14521 aacaatgcat tacttcgcat aggtatcgca cctttagccc atttgaagta tgaacaacag
    14581 cttgtcttca aacaaaaaca agtagagcat gtaatggcga agattgctaa gatgccagaa
    14641 gttcctgtac ttccaacggt cggaatggca gaaccattcg gctaccgtaa taaagcacag
    14701 atccctgttc gccgtatcga tggacagtta gccaccggtt tttacaaaaa gaatagccat
    14761 gatctagtcc ctatcgaaga tttttatatc caagatcctg tcatcgatca agcaatcaaa
    14821 gtagtcagag atatcttgca acggttccaa gtacgaggat ataatgaaga aaaaaatgaa
    14881 ggacagcttc gtcacatcat tatccgtcgt ggtcattatt cccatgaaat gatggtcgtt
    14941 ttagttactc gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc tattcatgaa
    15001 gaattgccag aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac aaatgtgatt
    15061 ttaggagaca aagaaaaagt tttatatggc agagactata tcgaagatca gctattagga
    15121 aaaacctatc gaatctcagc aaagtcattt tatcaagtaa atactcaaca agcagagtat
    15181 ctgtaccaaa cagccatcga gtttgccgag ttagataaaa aagacattgt gatcgacgct
    15241 tattcaggaa taggtacgat tggcttgtct ttagcagaca gagtggataa ggtgtatggg
    15301 atggaagtta ttccagaagc aattgaagat gcacagttca atgctttaaa taataaaatc
    15361 gaaaatgttc attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg gcaagaaaaa
    15421 gggatcaaac catctgtgat cgtagttgac cctccacgaa aaggattaga tcatcatttt
    15481 attgaatcag caatcgaaat gtcaccagaa cgaatcgtct atatctcatg taatccggca
    15541 acttttgcaa gagatgctaa gttatttgca gaagcaggtt atgccgtgaa gaaagttcag
    15601 cctgtagatc ttttcccgca aacccatcac atcgagtgtg taggactgct tacacgtgtg
    15661 gaacgttgat atgacaagga ttagagcgat tcgggatggt ttccggattg ctcttttttc
    15721 gtgttgaaat gacgattttt gggtgagcgt agagttgatg gaaatgctat ggagctttga
    15781 gttgatagga gttacaccgt agacttgata ggttagatga ttttgataac ggttacgtga
    15841 tataatatat tatcgagttt tcaagggaga caaacttatg aagcaaattt ttatattttc
    15901 agatacacag aaagataata tttcttattt tgaacgcgag ccagcaatct tggtttcaat
    15961 gagtcgtagt gatttgccgt atttgaagga acaggatttt tcaaattatc cagctgttta
    16021 tgttttgatt ggtggtaata agcgttacgt gggtcaggca gcagggcaat caatttcact
    16081 tcgtttatct caacactttc tcaaggaata taaagcttgg gttgaatcag ttttattctt
    16141 tacaagagca gatgggaaaa tgtcaaaagc agatacagat tatcttgaaa aacgacttat
    16201 ccaagatttc caagaaaaat ctgactatga aatgatgaat tcaactgctg gtaatacttc
    16261 gtatattgat aaacttcaaa aagcaaaatc ggatcagcta tatgacacag tgtttgaaat
    16321 tatagatgaa attgcaaata tcgatttgtt tggtacttcc gaagacagtg aaacccaaat
    16381 ttatgaattt agttcatctg gacaatatga aattgagtat gatgggaaaa agatatcttc
    16441 gaaatctgct agaggtttat tggttgattt tgtttctcag ctatttgcgg atggaaacta
    16501 caacaatcaa ctgcaagaat taattgtcga tgagacgccg tcgttcgcta taattttagg
    16561 acgtaaacca tccatgtata acggaagacc aaattcagcc agggtagctg atggagtatg
    16621 ggtttatgca aatttcaaca aaaagaatgt cagaatcaaa attgataaac tagctcgtca
    16681 attggggatt ccggttaaaa taaagtggaa ttaatatttg ttatctcgcc tttagtaaat
    16741 ctatgctgaa ggcttttatt ttagaaaaat agtttgcgtt cgcgaaaata tttcatgttt
    16801 cgtataataa tgatagctca tagatgatat aattgaagat accagtagaa tagaggtgta
    16861 gtatgtcaat ttcttataat aaattgtgga agttattgat tgatttgaat atgaacaaaa
    16921 ctcaacttcg tgaaatatct ggcgtcagtc ccaatgtaat agcaaaactc ggtaaaaatg
    16981 agttggttgc aatggagtca ttattgaaaa ttgctgctgc cttaaatgtt gatgttagtg
    17041 aaataattag ttcagatgtt atagatgcgg aggatgttaa ataaatggca aaaaataaag
    17101 ttttaaacat cactgaaatt gaagatggtg taaaaaaaat actttcaaat aggggcgatg
    17161 atttcatcac tcaatttttg tccttgtacg atatccccaa aacgtcgatt acacgtgcaa
    17221 aatcaaaatt cgataatggt gaaccatttg tgattaagaa caaacttcat tatgaagaaa
    17281 ttgaaggcaa cgttgtcatt gcgattgatg cgattgctca atcaatcaaa gagcaaaaat
    17341 ctaaaccacg atatatcttg gtaaacgatt atttagatat tgctgcaatt gatacgaaaa
    17401 ctcatgaaac tttgaatatt ccaattgaag agctaccggc taatgctgac tttttcttag
    17461 cgtggaatgg cattgagaag gctgattatc aagctgaaaa tccagctgac cgaaaagctg
    17521 ctgaacgttt tgcaaaattg tatgatgtgg tggctaaaga taatcctgat gccaacgagc
    17581 atgcatttaa cttgttcttg attcgagtgc tgttcttgtt gtttgcagaa gatacaggaa
    17641 ttatgaataa gggttccttt acaaatgttc taaaaacaag aacggctgaa gatggttcga
    17701 attttaacga agtaatcaaa gaactctttg caatcttgga tattaatgag ttagctcgca
    17761 atggcaagga tgagtggcta cttgaatttc catatgttaa tggaaaactg tttggtgagc
    17821 cacatgtgga tttgaccttt tcaaaagttt caagagagct gcttattgaa gcaggggaat
    17881 tattgaattg gaatgaaatc aatcctgata tccttggcgc aatgattcaa tcagttgctt
    17941 cagcagaaga ccgtcacgtt gccggtatgc attatacaag cgtgccgaat atcatgaaag
    18001 tcatcaagcc gttattctta gatgatttga cagagtcatt tgaaacttta aagcttcgca
    18061 gtgaagaaaa cgagctgaag gacattactg aaaaaacaag aaacgataat aagagaacaa
    18121 tcatcaatga tctaagtgct ttgttgacgc gaatttctaa tattaagttt ttggatcccg
    18181 ccagtggttc tggtaatttt ctgatcattg cttataagga aatccgtaga cttgaaatta
    18241 agattttgca attgttgggc gacttacaaa ataatgattc catgccacta tcagtgattc
    18301 atcttggtaa ttttaatggg attgaattgg atgattttgc gcatgaagta gcaagacttt
    18361 cattatggat tgctgaacat caaatgaata aagagcttga agaagcaata ccaggtacca
    18421 ttgctgagtt gttaccgtta aaagatgctg ggaatatagt gtgtgccaat tctcttcgaa
    18481 ttgattggaa tgaggtgttg cgactcaaag aaaatcatga aatatatata atgggaaatc
    18541 caccgtatct gggcaaaagt cttcaaaatg ctgaacaacg tgaagatatg gaaaaacttg
    18601 ttggcgatat tcgaaaatat aaattgcttg attatatttt tgggtggttt tggaaagcta
    18661 aacaatatat tgcaacatct catggctcgt tcgcatttgt tactactaat tcaatttgtc
    18721 aaggtgaaca agttagtatc ctttggccgt accttttaaa agatgaggta agaatttcat
    18781 ttgcatatca gagctttaag tggaacaaca atgcaaagaa taatgccggt gtgactgttg
    18841 tcattattgg tatgacaaat aatgtgaata ttcatcctaa gttatattta gagaatgggt
    18901 cagtaatgag tgtttcaaat attaatgcat atttagctaa tggtgaaaat gtatttgtca
    18961 acgaatcaag aaatagtatt tcaaatttac cctctttgaa atttggcagt atggcaaatg
    19021 atggtggtaa tctagttttt tcagtggcag aatactggaa tttgattc
//
LOCUS       DAZUKA010000050        18123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000050 DAZUKA010000000
VERSION     DAZUKA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.050"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1535)
                     /locus_tag="ACG8PN_001362"
     CDS             complement(27..1535)
                     /locus_tag="ACG8PN_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4861522.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1694..1885
                     /locus_tag="ACG8PN_001363"
     CDS             1694..1885
                     /locus_tag="ACG8PN_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861523.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2088..3005
                     /locus_tag="ACG8PN_001364"
     CDS             2088..3005
                     /locus_tag="ACG8PN_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4861524.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2998..4071
                     /locus_tag="ACG8PN_001365"
     CDS             2998..4071
                     /locus_tag="ACG8PN_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861525.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4219..4737
                     /locus_tag="ACG8PN_001366"
     CDS             4219..4737
                     /locus_tag="ACG8PN_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861526.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4718..5110
                     /locus_tag="ACG8PN_001367"
     CDS             4718..5110
                     /locus_tag="ACG8PN_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4861527.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5186..6121
                     /locus_tag="ACG8PN_001368"
     CDS             5186..6121
                     /locus_tag="ACG8PN_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4861528.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6254..7033
                     /locus_tag="ACG8PN_001369"
     CDS             6254..7033
                     /locus_tag="ACG8PN_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4861529.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7026..7343
                     /locus_tag="ACG8PN_001370"
     CDS             7026..7343
                     /locus_tag="ACG8PN_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4861530.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7587..9197
                     /locus_tag="ACG8PN_001371"
     CDS             7587..9197
                     /locus_tag="ACG8PN_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4861531.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9354..10202
                     /locus_tag="ACG8PN_001372"
     CDS             9354..10202
                     /locus_tag="ACG8PN_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4861532.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10433..10912
                     /locus_tag="ACG8PN_001373"
     CDS             10433..10912
                     /locus_tag="ACG8PN_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4861533.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10945..13173
                     /locus_tag="ACG8PN_001374"
     CDS             10945..13173
                     /locus_tag="ACG8PN_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4861534.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13302..14945
                     /locus_tag="ACG8PN_001375"
     CDS             13302..14945
                     /locus_tag="ACG8PN_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4861535.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15018..15563)
                     /locus_tag="ACG8PN_001376"
     CDS             complement(15018..15563)
                     /locus_tag="ACG8PN_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4861536.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15586..16071)
                     /locus_tag="ACG8PN_001377"
     CDS             complement(15586..16071)
                     /locus_tag="ACG8PN_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4861537.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16061..16873)
                     /locus_tag="ACG8PN_001378"
     CDS             complement(16061..16873)
                     /locus_tag="ACG8PN_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4861538.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17012..>18123)
                     /locus_tag="ACG8PN_001379"
     CDS             complement(17012..>18123)
                     /locus_tag="ACG8PN_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4861539.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5887 a         3300 c         3627 g         5309 t
ORIGIN      
        1 tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga agggtagaca
       61 ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt tttattgacc
      121 atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata agcaacttga
      181 gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt gatgcttagt
      241 gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc aagaactaat
      301 ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc atttaagata
      361 actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac ttcatctggt
      421 aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg aatcttatct
      481 gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc gacttcttcc
      541 ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt gaagtcgcat
      601 tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac aacggtatca
      661 tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg ataaacagat
      721 tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc aacatcccga
      781 ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat catattgaca
      841 tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt caacggaaca
      901 ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt ttggctttca
      961 tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg agctgtcact
     1021 acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc ttctgagacg
     1081 attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac cttgatgttt
     1141 cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata gcgagtgata
     1201 acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg caagatatcc
     1261 atatcataag taatcccata atcaccacgg atcttattac gttcaatatc tccagcatag
     1321 acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat tttcgcatct
     1381 tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt cccaccaaat
     1441 tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga ctgctcttca
     1501 atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta agtactaaag
     1561 cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa
     1621 agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata gtttttgata
     1681 aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac actatatccg
     1741 ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac aattgacgtt
     1801 tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca ttcatttcac
     1861 aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt cgattctgtc
     1921 ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct
     1981 gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa tggtactatt
     2041 gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg aaagagccag
     2101 atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat caggatacag
     2161 gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat ctttcccaat
     2221 tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc cctgagatct
     2281 accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg
     2341 agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa tttcctttta
     2401 tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc acttattacc
     2461 atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt ttgcctaaga
     2521 tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac aaaaaaatca
     2581 atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta gatgaacaaa
     2641 taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc ttgaatcgta
     2701 aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta tctaatgaaa
     2761 aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa aaagaagcat
     2821 tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc
     2881 aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat gaattcttga
     2941 tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt tccgagtatg
     3001 aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga tcatgaagaa
     3061 acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga agaaatccct
     3121 atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa tacgaatact
     3181 gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga aggaaaagag
     3241 caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt agcggattta
     3301 cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc atatgatcgt
     3361 tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat cgaatacttc
     3421 acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa ttcagcttat
     3481 gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca aaacgaatta
     3541 gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc gatgagtctc
     3601 ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt gctttctttg
     3661 atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat attcaatcac
     3721 aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa tcagacatta
     3781 ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa
     3841 gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat ggaaaagatc
     3901 aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat aacaacaaac
     3961 gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc tgtcattgat
     4021 ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg attggataag
     4081 ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc agttatccag
     4141 aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa
     4201 ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct
     4261 gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct
     4321 atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt
     4381 tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc
     4441 agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg
     4501 aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg
     4561 accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg
     4621 gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca
     4681 gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt
     4741 gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc
     4801 ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga
     4861 aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg atttactgag
     4921 tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg
     4981 gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc
     5041 tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc
     5101 taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt
     5161 cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt
     5221 aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat
     5281 cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca
     5341 gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt
     5401 tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg
     5461 cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt
     5521 gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc
     5581 gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg
     5641 ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc
     5701 tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc
     5761 tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt
     5821 cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc
     5881 aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt
     5941 tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga
     6001 gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga
     6061 caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata
     6121 aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa
     6181 acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa
     6241 aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt
     6301 gatgccactt tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa
     6361 agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc
     6421 gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat
     6481 ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa
     6541 aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc
     6601 ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt
     6661 caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc
     6721 ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat
     6781 gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt
     6841 aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat
     6901 tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact
     6961 aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa
     7021 tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc gtggcatata
     7081 tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg
     7141 actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca
     7201 ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag
     7261 caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg
     7321 tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata
     7381 agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt
     7441 attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg
     7501 aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt
     7561 atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc
     7621 taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa
     7681 gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc
     7741 ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa
     7801 aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta
     7861 cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc
     7921 ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa
     7981 gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag
     8041 gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga
     8101 tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag
     8161 ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt
     8221 atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt
     8281 atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta
     8341 tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac
     8401 tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg
     8461 cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta
     8521 taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa
     8581 cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt
     8641 ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat
     8701 atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga
     8761 tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc
     8821 tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg atgagcttat
     8881 caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat
     8941 accgaatagc acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc
     9001 tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg
     9061 aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat
     9121 ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata
     9181 gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc
     9241 gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt
     9301 atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac
     9361 ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact
     9421 tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata
     9481 tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac
     9541 aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca
     9601 cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga
     9661 tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc
     9721 cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg
     9781 aaatacttgc acatatcgat cgaatccgag ataataccta cgcttatctg attgtagatg
     9841 atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct
     9901 tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa
     9961 tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg
    10021 aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg
    10081 cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg
    10141 gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat
    10201 aattcctttt attcaaaaaa cataccaaaa atattccaac atcatcttgg actttctaca
    10261 tgttactaat gagcgtgtaa aagaacgaga tggtgttttt atttctatca atgaagctct
    10321 ttttctttgc aatcctcact tcaatcctct ataatgtcgc atatagttta gtaaatgagt
    10381 aaactactaa tttacttatt tactaaaaat aaaaggatga ggagttaaaa taatggaaat
    10441 accaacaaat ttaaaacata aaccagtaat cgttgtagaa gactatgata aagttgatgg
    10501 aagaaatgca ttaaatacgg atgcaaaagg gctttcactg ggccttgctc agtggaatga
    10561 tcgtggaaaa gtcgatatct caggcaaggt atggcgacat actggagaaa aatggtcacg
    10621 acagtcggaa gaattaccaa tcacacgtat tttagattta gcgatcttag ctgcacaagg
    10681 aagtttatac ttccaagatg cttatcgtta tgaaaaattt tatgatccag aaaatccagt
    10741 cgtagacatc attggattac aagggaaccg aatgacggta gaagtagata ccaaaaatcc
    10801 aaagatcgat gaagacatca ttttgtatta cgatacacta caaaaagacg gagaactgat
    10861 tggggaacga ttccgtattt taaaacgtct tttagatgag ctaggatatt aatagacaga
    10921 gatgatcaaa aggggaagag aagcatggag aatttctcac taacagaaaa ccgaaccttt
    10981 tcacggctag aaaagcaaat gatctggaag aaaccgaaaa gccatcggac aagtgcagaa
    11041 gaatggcgga tcgcctccga aatcaaaggg aattggcaag atcctgaaat gaaaatcagt
    11101 aacgtacttt tggaaggaga tgccgggtcg gggaagacac aattagccaa agcactatct
    11161 gctgatctgc aacttcccta tacaaaagtg acctgttttg cagatatgga caaatcagat
    11221 gttttcggag ccttattacc tgtagtagat tctaataagg aacaagacca agaattacta
    11281 gaagctattt accaaacaga tacacttgaa gctgtactga cactgatcca gaaccattac
    11341 ggcattgatc ctttttctgc taaagaaaaa ttagcagagc tagttcaaag gatcgaagct
    11401 gattatccag atgcaatcca ttacagctat tacccttctg aaatcgttcg agcgattgaa
    11461 aaaggatatt tattagagat tcaagagccg actgtcatcc gagatgcctc tgttcttgtg
    11521 gcacttaact cagcgctcga accaaatggg atgttgaatt taccgacagg aatcattcgt
    11581 cgccatccag attgcgtcat tgtgatcact acaaatcgaa attaccaagg aaatcgccca
    11641 ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa aaatggatct tcctgcctta
    11701 gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc aaaaacaaga acttctctta
    11761 aagatggcag agattatccg tttgttggat gaaactgcaa aagctaacgc catcaaaggt
    11821 gtagcaggaa tgcgttctta tttttattgg gcaaatacct ataagcaagg acaagattta
    11881 ctccagtcga tttatccaaa agttttgtat aaattatcca ctgattcaga tgaattggca
    11941 attcttgaac aagctttgtc tgacagtgga ttgctactgg aacttcagga catattgaga
    12001 aaagaaaaat ggggtcatcc tcaatctgag catacaaaag gaagaaccat tagcgccgaa
    12061 gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa ttctgcgctt agatgaaaag
    12121 aaatcgttgt ctgctgaaga accaaatgag ggagaagaga aacaagaaga aacaatagaa
    12181 aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta gcgatatcga aggaaaaaat
    12241 caaaaagaac aacaaacagg agatgattct caagaaatgt ctgcttctga catggaagca
    12301 catgataagg aaatcaagaa gcaactgaat aaagaagcgc gacaaatcat gaaggaaacg
    12361 attcatgaaa aagaaggtct catcgttcac cgaccagctt tccaaggaaa aacgccggaa
    12421 gcacgtgagc tacacatgca agtaatgcca atagtagaat cacttagccg gcaaatttta
    12481 gagttactgg ataatgaaca aacagagacg taccaaaaag gaaaatacga aggtcagcgg
    12541 ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt ttgacaaaaa gaacccgcca
    12601 catgagcaac cttctttagc ggtagctgta cggatcgatg aatctgggtc aatgatacgg
    12661 gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca ttgccgaatt ttctaagaaa
    12721 gtcgatatcc cattgttgat ttacggagat acagcagacc gttcgtcaag agaaaagact
    12781 tctttgtttt cttataaaga atttgaagac ggatttcaat ggttaaatga aaaattagtc
    12841 actatgaaac caaggcaaaa caatcgtgat ggtgctgcac ttcgtttagc agcagaaaaa
    12901 ttaaacagac aatccgcaac aaccaaactg ttgcttaata ttagtgacgg tcagccaaaa
    12961 gcgttgccgg attatacagg caaaaaagca aaagaagata tccagtctgt tttgaaagaa
    13021 tatgaacgtc aaggaatcct atttgtctcc gcagctattg gccaagacaa agaagaaatc
    13081 aagtcgatct acggtgaaag tcgttttgta gatattactg acttgaatga atttcccaag
    13141 caaatgattc aactgattgc ccgctatcta tgataaaata aagctcaacg aacgcactta
    13201 gcagattcgt tgagcttttc gtttctagtt accggtaaca aggatacttc taaaatagaa
    13261 tcggttataa tcaagaggga ccgttaaagg agtgaaacga tatgatacaa gaacaaaaat
    13321 ggtggaaaga agcagtaggc tatcaaatct atcctgctag tttcaaagat agcaatggcg
    13381 atggtcaagg agacatcaat gggatccgtg aaaaacttaa ttatttgaaa gatcttggta
    13441 tcggctttat ctggatcaat ccgatctatc aatctccatt tgtcgataat ggttatgacg
    13501 taagtgacta tcaagatata gaagagagat tcggaacgat ggaagatttt gatttattgc
    13561 taaaagaagc acatgaatta gggatcaaag tgattatgga tcttgtcatc aaccatagtt
    13621 cggatcagca tcaatggttt gaagaatcga aaaaatctaa agaaagcccg tatcgcgatt
    13681 attacatttg ggtcgacggg gtagatggca aagaaccgaa taattggaca tcgattttcg
    13741 gcggttctgc ttgggagtat tctcatgaaa ctggacagta ttatctgcat gtttttgcaa
    13801 aagaacaacc agacttaaat tgggaaagcg agaaattgaa agaagaacta ttcaatatga
    13861 tccgatggtg gttggataaa gggatagatg gatttcgttt agatgcgatt tctcatgtaa
    13921 aaaaagatga atattcagta aaagccacag agaacccttt ctcacccttt caaaatgtct
    13981 caggaatcga agaacatttg accgatttga aacatgtttt tgaagagtac gatatcatga
    14041 ctgtcggaga agcaagtggt gtcactgcag aagaaggacc acaatgggta gggaaagacg
    14101 gttatttcga tatgatattc gaatttgatc atatccatat ctggcagcaa gaaaaagaag
    14161 ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc ttggcaaact tcattggatg
    14221 gtatcggatg gaacgcttta tatatggaaa accatgatgt tcctcgtgcc gtttcagtgt
    14281 ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa agcaatcgct atgatgtatt
    14341 tctttttgca aggtacgcca tttatttatc aaggacagga aatcggaatg acgaatatgc
    14401 cctttgaatc gatcgatcaa gttgatgcag tagattcgaa aagactttat aaaaggctgc
    14461 ttgcggaagg taagacaaga gaagaagcat tggatatcat tcgtgaaaca acgagagaca
    14521 acagcaggac acccatgcaa tggacttcgg aacaatatgc tggtttttct acacatgaac
    14581 cttggctgat cactaatcca aataccaaaa cgatcaatgt agaacaacaa gaatatgaac
    14641 cagagtcggt cctgcaattc tataagaaca tgatccgcat tagacaaacc aataaaggtc
    14701 tgatatacgg ttcttataaa gaatatcttc atgaacatcc acaactttat gtctatgaaa
    14761 gatatttaga agatgaacaa tatcttatta tggtaaactt aacagaatca cttgctgact
    14821 atgaacttcc aaaagaagca gatcagtcct ggacattact cttgtcaaat tcctctagcg
    14881 gagaatttga agctaaaggc atactagcac cttatgaagc tcggttatat aaaacaaaca
    14941 agtaaaactt cctcgttaaa gttcattagt tggactttgc gaggaagttt ttcagattgc
    15001 tctgtcaatt actcatttca ataattcgct ctaggaccac cgatatattt aggacgagag
    15061 cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac gaggtctgac aggtacttgg
    15121 acatgtttga tatgcatacc gattgatgtg tcaccgatat cgataccggc ttgtccaact
    15181 acgtgttcca cttcgacagg atcattgaac tggtcaaaag cagcaacaga acaagcacct
    15241 ccagcatgta gtgcaggaac aacagagaca atttcgaaat tttttgcttc tgccactgtt
    15301 cgttctacta ctaatgctcg gttcaaatgt tcacaacctt ggacagctag cgcaatttct
    15361 ttaggatcca acaactcttt taacgtacgg atgatccatt gaccaacttc cgcactggaa
    15421 ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc atcctaaaac aaaaagatca
    15481 ccttttttca agtgtgcctc agccaatatg tcattaacta tttcagtcaa ttggtctttt
    15541 aattcttttt ctgacaactt cattttctaa aaccttcttt ttcaattaat attgcttccg
    15601 tgcttgaata cgttgaagac ttgcagttat aggaaaggca agtcccattc caatcagatt
    15661 ttggaagatg tttcctggta tagaagccac tccagccggc caatcatata aaatccaacc
    15721 ggcgatacca tagccgatga tcataactaa gcttcctaat aaaagcccta atccccacat
    15781 acgagtagaa gatttgctga aagaaccagc aacatatcct tgtaacccgt ggatcactaa
    15841 tgaaaaaact gcccactgcg gatatcctga aatcaaatcg atcaatagac cactggaagc
    15901 accaacaact aatccacccg ttggaccata aagaaaagca gcggtataga tcccggcctc
    15961 gcataatgtc acaaatccat tcgtagccgg tacagggatc aaaattgtta atgaaagggc
    16021 aactgtcaac gcaatcagca tagcatagat cgttaattgc ttagttttca tttctcattc
    16081 ctcctttttg aataccgcca gaccagacac ttccggtatg atcgttcatc agcacgccat
    16141 tagaaataca atgggcaaca aattttttac tttctgctac tgctttggta aaatctgccc
    16201 ctaacacaag attcgctgtt attgcagaag aaaaggcaca accggccccg tttactgttg
    16261 tagtgtcgat ttttttatga gaaaatacct cataatgatc aattgaatag agaagatcca
    16321 cagcttcatc tccagccatc cttgtgccgc ctttgatcac aatgtttttt gttcctgaag
    16381 cagtgagttc tttagcgaga tggatcattt ctgttaacga agagggaaga gcttgaccag
    16441 aaagtaaagc agcttctgcc aaattaggcg taatcatcgt aacaagtgga aaaagttctt
    16501 cgatgagctg cgaaatatac aattgattga tcacagcttc agtttctttg aatgccaaaa
    16561 cagggtctaa aatgattggg atattttgtt ttgtttttat gaagtctcga acaaccatga
    16621 ttgcttcact tgaattcaat aacccaattt tgataccatc taatgaagtc attttatcaa
    16681 tcgaaactaa ctgtcgttta agcaattcca aagatataga ttccagcata aatcgctcat
    16741 taatatctag tgcccctata gaagtcaagg cgcttagtcc gaatagacca aaattctcaa
    16801 aagtttttaa atctgtttgg attcctccac cagcaaatgg atcagaaccg ccaattgtca
    16861 agattctttt cataacattt tcctccatga ataagagtat aacgaaattt tcaaaaaaga
    16921 aaaacagcca attggcatat ttttatctca tccaatttaa aaaaacaaaa agaaaagaaa
    16981 gaccaaaatg gatctttctt tccttaaata gttaggactt ttgtgatttt aattctttct
    17041 cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg gattgcatca aaatcacgga
    17101 tgatagggac gttcaagcga ggaagttcat ggacttgctc ccaccatcga ttccaatcca
    17161 tttctttatc aggagctaaa gcaataatcg gttgtggaga atgattttta tcccttacaa
    17221 tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa gtcttcaata gctaatgtac
    17281 tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa aaataggtgg ccatgcttat
    17341 ccataaaacc gtaatctccg ctatcccacc aatcgccata aacattttcc tggaaccgtg
    17401 catcttcctt gtaataagtc aaagcccgtc ctttagacag taattgtata tgaccatctg
    17461 tgttttcagg taggacattc ccaactgaat ctgtaatccg agcgcttgtc atgtcttcca
    17521 gtccaacgcc catatctctt gcatcacttg ttttcaaact ctctagtgta tgagccttaa
    17581 gaatcattgg tccgcattca ctttggccat atacttgaag aaataccgca tcctgtgcca
    17641 tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat agcatcaaaa gtggagtgat
    17701 agtatcggat acccgaaaaa gcttctggat gctccttagc agtaaaacgc cactgaacaa
    17761 agttattcgg atgggtctct agtgcaattg gccggtgagc ttccaacata tgaacaactt
    17821 ttgaactttg tgcgtttgct aaaggcatca taggaaatcc catggacatc aaagaagaga
    17881 taccaatatt gaatctggaa tgcactggag agatgtgaaa agcgattaat ttcttttccg
    17941 aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca tcccatagaa tgagcagaat
    18001 gacaaataag tttagggata ccagttgtac cagaagtatg ggtcatataa gaaatttcat
    18061 ttttgtctaa aaatgattga gaaacagaca aagcaggttg ttccattaaa taaagaatag
    18121 gaa
//
LOCUS       DAZUKA010000051        17733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000051 DAZUKA010000000
VERSION     DAZUKA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.051"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..834
                     /locus_tag="ACG8PN_001380"
     CDS             22..834
                     /locus_tag="ACG8PN_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4861540.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            831..1631
                     /locus_tag="ACG8PN_001381"
     CDS             831..1631
                     /locus_tag="ACG8PN_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4861541.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1792..3060
                     /gene="rseP"
                     /locus_tag="ACG8PN_001382"
     CDS             1792..3060
                     /gene="rseP"
                     /locus_tag="ACG8PN_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4861542.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3128..4837
                     /locus_tag="ACG8PN_001383"
     CDS             3128..4837
                     /locus_tag="ACG8PN_001383"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4861543.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5049..9401
                     /locus_tag="ACG8PN_001384"
     CDS             5049..9401
                     /locus_tag="ACG8PN_001384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4861544.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9545..10018
                     /gene="rimP"
                     /locus_tag="ACG8PN_001385"
     CDS             9545..10018
                     /gene="rimP"
                     /locus_tag="ACG8PN_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4861545.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10042..11217
                     /gene="nusA"
                     /locus_tag="ACG8PN_001386"
     CDS             10042..11217
                     /gene="nusA"
                     /locus_tag="ACG8PN_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4861546.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11239..11532
                     /locus_tag="ACG8PN_001387"
     CDS             11239..11532
                     /locus_tag="ACG8PN_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4861547.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11529..11840
                     /locus_tag="ACG8PN_001388"
     CDS             11529..11840
                     /locus_tag="ACG8PN_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4861548.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11853..14159
                     /gene="infB"
                     /locus_tag="ACG8PN_001389"
     CDS             11853..14159
                     /gene="infB"
                     /locus_tag="ACG8PN_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4861549.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14186..14533
                     /gene="rbfA"
                     /locus_tag="ACG8PN_001390"
     CDS             14186..14533
                     /gene="rbfA"
                     /locus_tag="ACG8PN_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4861550.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14641..15145)
                     /locus_tag="ACG8PN_001391"
                     /pseudo
     CDS             complement(14641..15145)
                     /locus_tag="ACG8PN_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15310..17673
                     /locus_tag="ACG8PN_001392"
     CDS             15310..17673
                     /locus_tag="ACG8PN_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS4861551.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6046 a         3147 c         3907 g         4633 t
ORIGIN      
        1 ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa atatattcaa
       61 gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat tgccatcatc
      121 atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa
      181 gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg
      241 ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga agtaagtttt
      301 ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat taaagaaaat
      361 gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca agatgctgta
      421 agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt tgctttgaat
      481 tatggaagta gagcagagat cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa
      541 ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt aatgacaggt
      601 ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg agaagagcga
      661 attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac aaaagcgctt
      721 tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca aaatcgagac
      781 cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc atgaaacaac
      841 gtgtgatcac agcagctgtg gcgctagtct tgtttattcc gattatatgg atcggaggca
      901 tagcggttga gcttacagca gcaatcttag cggtagtagg agtttacgag ctgttccgga
      961 tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt gcagtgttac
     1021 ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt acatttatgt
     1081 tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa aatacgtata
     1141 cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata ggtttccaga
     1201 attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta tttattgttt
     1261 gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat aagttaagtc
     1321 ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtatcggt agtgctgttg
     1381 tagtcgcatt tctttattta ttgctatacc cagcaaaaga actattcaat catggtatgt
     1441 ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt gatttggtcg
     1501 aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta ccaggccatg
     1561 gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg catttatttg
     1621 gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac gcaggttttt
     1681 atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa ttatagtgtt
     1741 ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg
     1801 attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt tggtcatttc
     1861 ttctttgcga aacgatcagg aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa
     1921 atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt acctattggc
     1981 ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc aggaatgcct
     2041 ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag taagaaaatc
     2101 caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga tgagctgact
     2161 atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt tgatcacgat
     2221 gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga cgtacagttc
     2281 caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat gaataacttt
     2341 attttggcaa tcgtgttatt catcattctg gcatttatgc aaggtggtgt tcaagtgacg
     2401 aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc aggcttaaaa
     2461 gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa tgatctgaca
     2521 acagtcatta cgaaaaatcc aggtaaaaca ttagacttta agatcgaacg agaaggtcag
     2581 gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa agttggccaa
     2641 cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg agggacgcgt
     2701 caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt tacaggtttc
     2761 agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga agcagcaaat
     2821 caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct cggaatcgtc
     2881 aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat ttttgaaggg
     2941 attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc tggctttgga
     3001 tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt ctttttctag
     3061 aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa ataaaggagt
     3121 atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc caaatgatgc
     3181 agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag tatcagcggg
     3241 tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac gcattatgcg
     3301 ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct tgccagcaga
     3361 actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc gtttcaaaga
     3421 ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta ctgatttgat
     3481 gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa tccaacctaa
     3541 atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa
     3601 agatgcatat tctttccatg caacggaaga aagtttggac gagacatata aagattacga
     3661 aaaagcctat accgaagtat tcaagcgttg tggcttagaa tttcgctcga tcatcggaga
     3721 cggcggcgca atgggcggga aagactcaaa ggaattcatg gcgatctcag aaatcgggga
     3781 ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga tggctacaag
     3841 ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag tagctacacc
     3901 taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc aaaaaatcat
     3961 caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac gtggagacca
     4021 tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag aagaagcaac
     4081 agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac cagtcggtgt
     4141 ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga atgccattac
     4201 tggagcgaac gaaacaggat tccactataa aaatgtgaat ccaaaacgcg atttcactgt
     4261 gctttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag acaacaacgg
     4321 tgttcttgcc tttactcgag gaatcgaaat cggacatata ttcaaaatcg gcactcgtta
     4381 cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat
     4441 gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac aacattcaga
     4501 tgaacaagga atcaattggc caaaaggaat tgcgccattt gatctgcata tcgtccaaat
     4561 gaatctaaaa gacgaatacc aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa
     4621 agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga aattcgcgga
     4681 ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag tggatggaat
     4741 cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag aagaattggc
     4801 gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac acagaaaata
     4861 aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa ttggaactgc
     4921 gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa
     4981 ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc
     5041 gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag
     5101 aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc
     5161 atcgtaaatc gcgtttatgg gaatttacgc tacatttttc caaaattttg cctattatgc
     5221 tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac
     5281 tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc
     5341 aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg
     5401 tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt
     5461 atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac ggattcccaa
     5521 aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgtgtattg aaactttttg
     5581 aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg
     5641 tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc
     5701 agctaggacg aaatattcca gccgatgaac caacgacacc aatgatcagt atcgttgaag
     5761 aagaacgaag agtcacactt gaggggtacg tattcgacaa agaagtcaga gaactacgtt
     5821 caaaacgcaa gatcttgact ttgaaaatca cagattatac ttcatcattt atcgtcaaaa
     5881 aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc gatcagtgtc ggtagctggt
     5941 taaaggtacg aggaagtatt caagaagata cgtttgtccg tgatttggtc atgaatgcgc
     6001 aagatatcat agaagtcaaa catacacctc gcaaagatta tgctcctgaa ggggaaaaac
     6061 gagtagagct tcatgttcat agcaatatga gtacgatgga tgcaacaaac agcatctctg
     6121 atcttgtggc acaagcagga aaatggggac accgagctat cgccattaca gaccatggtg
     6181 gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat
     6241 acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat tgcttataat gatgcacatg
     6301 aagcattgag tgaagccaca tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag
     6361 tatatgacac gatcatcgag ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa
     6421 gctttgatga atttatcgat ccaggacatc cattgtcacg aacaacggta gatctgactg
     6481 gtatcacgga tgaaatggtt cgagggtcaa agtcggaaga agaagtattg cggatgttcc
     6541 ttgaattttc aaaagataca attttggtag cccacaacgc ggcttttgat atggggttct
     6601 tgaatactag ctacgcaagg tatggtattc ctgaagcggc caatccagtc attgatacgt
     6661 tggaattagc tagatatctg tatcctcaat tcaaacgttt cggattaggt gtgttatcta
     6721 aaaagtttgg tgttagcttg gaacagcatc accgagcaat ctatgatgct gaagcaactg
     6781 gtcacttagc atggatcttt gtgaaagagg caatggacaa tcataacatg ctttatcatg
     6841 atcagctaaa tgagcatatc ggagaaggag attcttacaa aagggctcga cctttccatg
     6901 taacgatttt agcaaaaaat caggcaggac tgaaagattt gttcaaactg atttcaatgt
     6961 cgaatgtcga atacttcgaa cgcgtcccac gaataccacg ctcccaattg aaaaagatgc
     7021 gtgaaaatct attgatcggc tcagcttgtg ataagggaga aatattcgaa gccatgatgc
     7081 aaaaaggggt agaagaggca agaaaccgag caaaattcta tgattatatt gaagttatgc
     7141 caaaagcggt ctatgcgcct ttgatcgagc aagaattggt aaaaaatgaa catgatttgg
     7201 aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa
     7261 caggtaatgt acattatctg aacgaagaag atgccattta ccgaaaaatt ttgatcaatt
     7321 ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa
     7381 ccgatgagat gctaactgca tttcactttt taggggaaga aacagcgaaa gagatcgtag
     7441 tagaaaatac gaataaaatc gcagatatct gcgaagaagt gattccagtc aaagatgaac
     7501 tttatacacc gaagattcca ggttcagaag atgaaatatc agaattgagt tacacaaaag
     7561 caaaacaaat gtacggagat ccgttacctg agatcattca aaaacgatta gaaaaagagt
     7621 tgaattctat taatggtaat ggcttctcgg ttatttatct gatcgcccaa aaactagtgc
     7681 ataaaagtaa tgaagatggc tatctagtag gttcacgtgg ctctgttggc tctagctttg
     7741 ttgcgacgat gacaggaatc actgaagtta atccattagc ccctcattat tattgtccag
     7801 agtgccaata ttccgagttt tttgaagatg gtacgtatgg ttctggattt gacatgccag
     7861 aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga cggtcatgat attccatttg
     7921 agacattttt aggattccat ggagacaaag tgcccgatat tgatttgaac ttctcaggtg
     7981 attatcaagc agaagcccat aactatacga aagtattgtt tggcgaagac tatgtttacc
     8041 gtgccggaac gattggtaca gtcgccgaca aaacagcata tggctatgta aaaggatacg
     8101 aaagggacaa taatctgcag tttcgaagtg cagaagtcga cagattagca aaaggcgcta
     8161 ctggagtcaa acggacaacc ggccagcatc caggggggat catcgttatt cccgattata
     8221 tggatgtcta tgattttacg ccgatccaat atccagcaga cgatcaaaat tcagaatgga
     8281 aaacgactca ttttgatttc cactcgattc acgataatgt attgaaatta gatattctag
     8341 ggcatgatga cccaactgtt attcggatgc ttcaagattt atcagggatc gacccacaaa
     8401 cgatccctac ggatgacccg gaagtgatgc ggatatttgc cggtcctgaa gtacttggag
     8461 taagtcagga acaaatttat tccaaaacag ggacattagg tattccagaa ttcggcactc
     8521 gttttgttcg agggatgtta gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa
     8581 tttccggact atctcatggt acagacgtat ggttaggaaa tgcggaagaa ttgattcgac
     8641 gaggagatgc gacactggcg gaagtaatcg gctgtcgtga tgatattatg gtttatttga
     8701 tccatgctgg attagatagc gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac
     8761 aatggaacaa gatccctgat gaattgcgag aaacctatct atcggcgatg aaagaaaata
     8821 atgtgcccga ctggtatatc gattcttgtt caaaaatcaa atatatgttt ccgaaagccc
     8881 atgctgcagc ttacgtattg atggctcttc gtgtcgctta tttcaaggtt tatttcccta
     8941 ttttgtatta ctgtgcatac ttttctgtac gtgcagacga ctttgatcta gtctccatgt
     9001 gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat cacggacaag ggattagatg
     9061 cttccgtaaa agaaaagaat caactgacag tgctggagtt agccaatgaa atgctggaac
     9121 gaggattcaa atttggaatg attgatttgt ataaatcgga tgccgttaac tttgttattg
     9181 aaggtgatac gttgatcgca ccatttagag ctgtcccaag tttgggaacc aacgtggcga
     9241 aacaaatcgt tgaagcaaga aaagacggtc catttttatc caaagaagat cttgccaccc
     9301 gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga caatggcgta ttgaaagatc
     9361 taccagatga aaatcaatta tcgttattcg atatgctata agcatctgtt ttcctgtctg
     9421 cttgtaattt taagcagagt atggtatagt tcttataggt aatgatacta actgtgagtg
     9481 agcggatttt tcgctcactc tttttaatgg aattacgcac ttaattttta aggaggcgaa
     9541 acttttgagt agcgtcgttg aaacagtcac agaaatggtg acaccgattt tagatgaaca
     9601 aaattttgaa cttgtcgaag tcgaatttgt aaaagaagga aaaagctggt tcttacgagt
     9661 atttattgac aaagaaggcg gtatcgatat cgaagaatgc gcctttgtca gtgagaaact
     9721 gagtgaaaaa cttgatgcag cagatccaga tccgattcct caagcttatt tcctggaggt
     9781 atcctccccg ggagcagagc ggccattgaa aaaagaaagc gactatgaaa aggcacttgg
     9841 tgattatgtc catgtctcct tataccaacc agtagatggc gaaaaacaat atgaaggatt
     9901 tcttcaatca tttgatgctg aacagcttac attgaaaata cgcataaaaa cacgagaaaa
     9961 agaattgact tttgaccgaa aaaatattgc aaaagctcgc ttagctatcc aattttaatc
    10021 acggagagga atcagaaaaa aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa
    10081 gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt tagaagcagc attggtttct
    10141 gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa
    10201 ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg
    10261 gaagtctcat tgaaagatgc acttttaatc aatccagcat atgagatcgg tgatacgatc
    10321 cgttttgaag taacaccaaa agattttgga cggatcgctg cgcaaacggc taaacaagtt
    10381 attttacagc gtgtacgtga agcagaaaga acgattattt ataacgaatt cagtgcttat
    10441 gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata aacgttatat ttatgtcaat
    10501 cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa tgccaaatga attttatcaa
    10561 ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa
    10621 gtatttgtta gccgcagcca tccagaccta ttgcgtcgct tatttgaaca agaagtacca
    10681 gaagtctatg atggaatcgt tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca
    10741 aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag tcggtacatg tgtcgggcca
    10801 aagggacaac gagttcaagc aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc
    10861 gaatggaatg aagatccagc tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg
    10921 gatgtgatct tcgatgagac aaatccaaag gcctgcacag ttgtcgttcc agactaccaa
    10981 ttatcgctag caatcggtaa acgcggacaa aatgcacgtt tagctgctaa attgactaac
    11041 cataaaatcg atatcaaatc agaatctgac atgacggaat tttatgaaaa tcaagaacaa
    11101 caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc agtcagattt aacagacgat
    11161 gaatatgaaa caattgcatt caacaatgaa acggtcgaag aaaaaccaga agcttaactt
    11221 tagtaaggag gcaaagagat gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca
    11281 ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc gttcaaaaga aggggaagta
    11341 tcgatcgatc caactggaaa aatgcctgga cgaggagcgt atgtctcact tgagccggaa
    11401 gaagtacaac aagcttggga taaaaaaatt ctcgatcgag tactcgaaac aacattaaca
    11461 gatgagtttt atcaagaact tcttgactat gtcactcacc aaaaagcacg taaagagtta
    11521 tttggaaaat gaatacagtt aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc
    11581 gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag
    11641 caaaattggt gtttgtggca accgatgcaa gtgagaatac aagaaaaaag ataaaagata
    11701 agagttcata ttatgaagtt ccttgctttg agctattttc tgaagcagaa atcacgcaga
    11761 tgatcggaaa acctcgcaag gtattcggaa taatggatgc cggatttgcg aagaagacca
    11821 aggagctaat tgaaggttag gaaggtgatt gcatgggcaa caaaagaatc tatgaattag
    11881 caaaagaact caaaaaatct agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg
    11941 atgtgaaaaa ccatatgggt gcgattacta gcagtgatga gaagaaattg caacaagcgt
    12001 tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc aagcagaaaa ccagccaatc
    12061 aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata
    12121 accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa
    12181 caacaaacca aaataatacg ggaaatacca gcagccaaaa tcgtcaagga acgagccaag
    12241 gcggcaatca aaaccgtact aacaataaca acaaaccaaa caataacaat cgcggcaaat
    12301 ttaataataa taaccgtaat cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat
    12361 caactaaacc agctgtacca ccacgcaaat tccgtgaatt gccagatgta ttagagtata
    12421 ctgaaggtat gaacgtagca gatattgcga aaaaaattca tcgtgagcca gcagaaatca
    12481 ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata
    12541 cgattgaact gttagcgacg gactatggca tggaaccaca agaaaaagtc caagttgaca
    12601 ttgctgatat cgataaattc ttcgaggcag aagaagtcga tccagataaa ttagtctcac
    12661 gtccgccagt agtaacgatt atgggacacg tagaccacgg gaaaacaacc ttattggata
    12721 cattgcgcca ttcacgtgta acaagcggcg aagccggcgg aatcacacag catatcggtg
    12781 cctaccagat cgatattgat ggtaaaccaa tcactttctt ggatacacca ggacatgcgg
    12841 cctttacaag tatgcgtgca cgtggagcaa gtatcacaga tatcacgatc ttagttgttg
    12901 ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat caaccatgcc aaagctgcag
    12961 gcgttccaat catcgtcgct gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg
    13021 ttatgcaaga attaagtgag tatgaattaa ttcctgaagc atggggtgga gaaacgattt
    13081 ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga attgctggaa atgatcctgt
    13141 tagtcgcaga agtagaagat ttaaaagcag atccaacaca acgcgcaatc ggaacagtga
    13201 tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa
    13261 cacttcacgt tggtgaccca atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga
    13321 caaacgatct tggccgtcgc gataaagaag caggacctgc aacgccagta gaaatcactg
    13381 gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag
    13441 cacgtgcagc gggtgaagaa cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa
    13501 gccgtgtgac tttggacaac ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta
    13561 acgtaatcat caaagcagat gtacaaggtt cagcagaagc actggctgca tcattgaaga
    13621 agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg
    13681 aaagtgatgt aaccttagca gcagccagca acgctattat catcggtttc aacgttcgtc
    13741 ctacaccgca ggcaaaagtg caagctgata ctgaagaagt cgatattcgt cttcaccgta
    13801 tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat
    13861 ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg
    13921 gtacgattgc aggtgcctat gtaacagaag gatatattcg tagagatagc ggtgtccgtg
    13981 taatccgtga cggaatcgta atctatgaag gccaacttgc aagtctgaaa cgattcaaag
    14041 atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc gatgattgaa aaattcaatg
    14101 acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa aatgactaaa
    14161 aactgataaa aggaggccaa tcattatggc taactaccgt gatcgccgag tcggtcaaga
    14221 aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa
    14281 cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga tctattacag
    14341 cattttatct gacttagcat cagataagaa aaaagcacaa gatggattga ataaagcatc
    14401 aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac ctgaattgtt
    14461 ctttgaactt gatgaatcag ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt
    14521 gaacaaagac taatcaaaaa atcccgcaaa tcctgtgaaa tcaggggttg cgggattttt
    14581 ttgttttcat taagacgtat tacctaagag ttccatgact ttagtctggt agcgtgtcag
    14641 ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact ttcttttttc tatagtatat
    14701 aaatttttgc atatagataa agatcaagat accgataacg gaagctagat tgtaatctat
    14761 tttagtgaca gtcaggtata gccaagtaac tagaagatca gtggaacctg caccataaaa
    14821 gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc acgttgaagt tattgatatt
    14881 tccgattacg ccgcttcgat ttggtcacga tccaaatttt ggataatccc tgtagaaatt
    14941 agcatagtag ctgggatacc aattcacata ttgacaaaga taacagagac ttttacataa
    15001 aacggatcaa tcaggaaagg aattgctcga tccagcccca gttttccaac aatgtgttaa
    15061 ctggcctagc gtcattcagc aaattatgca tgatgaggag cgaaatgaac tgcggaacag
    15121 ccattgtgat aacgacaagt tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat
    15181 ttcgtggttt tttatacctg ttcatttggc tagatataaa atccaaacaa atgaacagaa
    15241 gtgcttatta ggtttcaatt gggagtatct gcgctagaat aagagtaata atgaaaagga
    15301 gtgttatgta tggattattc tagtaaagaa tactttgata aaatgacggc ttggtggcgt
    15361 gctgctaatt atctttctgt tgggcaactt tatcttaaag ataacccctt actgagacgt
    15421 acactgaaac cagaagatgt caaaaaacat ccaatcggtc attggggaac gattcctggc
    15481 caaaatttta tttatgtcca tttgaaccga gtcatcaata aatatgattt gaacatgttt
    15541 tatatcgaag ggccaggaca cggcggacaa gtcatggtat cgaatgctta tttagatgga
    15601 agttacacag agatctatcc agaagtaacg gaagatgaga caggaatgca aaaattattc
    15661 aagcgatttt catttcctgg aggcattgct tctcatgcag caccagaaac accaggttct
    15721 atccatgaag gcggagagct tggatactct ctttcacatg cagtcggagc tgtattagac
    15781 aatcctgaag taatctctgc tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta
    15841 gctggaagct ggttctcaaa tgtctttatc aatccagtga tagatggagc tgttttaccg
    15901 atccttcatt taaatggtgc aaaaatagcc aacccaacga tcttggcacg taaatcagat
    15961 ggagaattag caaattactt taacggactt ggctgggagc ctttctttat tgaaggaaat
    16021 gatccagaaa aattaaatcc tgttatggct gaaaaaatgg atcaagcaat cgagaaaatc
    16081 aaatccatcc aaaaagaagc tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg
    16141 ccagtattga tcgtacgcac accaaaagga tggaccggtc caaaagaatg ggacggggaa
    16201 ccaattgaag gaaccttccg agcacaccaa gtaccgattc ctgtagatca agaacatatg
    16261 gatcatgcag atgctttact ccgttggctg aaatcctatg aaccagaaaa attatttgat
    16321 gcacaaggac gtattttaga agaaatcaga gaaattgcgc ctactggtga ccaaagaatg
    16381 gcaaaaaatc ctatcaccaa tggtggaatc gatccaagac cgcttattat gccagattgg
    16441 aaaaaatata ctttgcaatt tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa
    16501 cttggtaaat tcgttcgcga gatcattgag aaaaatccag aaaatttccg tatatttgga
    16561 ccagatgaaa caaaatcaaa ccgattgaat caagttttca aaacgacaaa tcgccaatgg
    16621 atggaaaaaa tcgaaccaga aaatgatgaa tggctttcgc catctggtcg cgtcatcgac
    16681 agtcaattat cggaacacca agatgaaggg ttcttagaag ggtacgttct gactggacgg
    16741 catggttttt ttgcaagtta cgaatcattc ttgcgagtgg ttgactctat gttgactcag
    16801 cacttcaaat ggatacggaa atcacatgac ctttcttggc gaaaagatta tccttcgctg
    16861 aatctcattg caagctcaac agtcttccag caagatcaca atggatatag ccaccaagat
    16921 ccaggaatat tgacacattt ggcagaaaag aaggcggagt tcatccgtga atatctgcct
    16981 gcggatgcaa atacattgct cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc
    17041 aatttgatca tctcttccaa acatccgcgt gctcaatttt actcagcaga agaagcagcc
    17101 gtcttagtga atgaaggctt gaaaatcatt gattgggctt cgacagcaaa agaggaggaa
    17161 cctgaattgg tcattgctgc tgcaggaacg gaatctaact tagaagcatt agcagctgta
    17221 actttgctgc tggaagaatt cccaaaacta aaaattcggt tcatcaatgt cgtagattta
    17281 ttgaaattac gtcatccaag tcaagatccg cgtgggttga gcgatgaaga atttgatcaa
    17341 tattttacaa aagacaaacc gatcttattt gctttccacg gttatgaaac attggtccgc
    17401 acgattttct ttgatcgtca taaccatcat ttaatgattc atggctacaa agaaaatgga
    17461 gatatcacga caccatttga catgcgtgta gtaaatgaac ttgatcgtta tcatttggca
    17521 aaagacgcag ctttaaaaat caaaggaagt caagcagaag attttgcgga aaaaatggat
    17581 cagaaattac aagaacatca aaactatatt cgtgaaaatg gtattgatct gccagaagta
    17641 ttggattgga aatggaaaaa tcttgaccaa taaaatgata aaatcgatcc cgcaaaacag
    17701 aattttttac attgtattat ttgtcaaatt aag
//
LOCUS       DAZUKA010000052        17710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000052 DAZUKA010000000
VERSION     DAZUKA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.052"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..867
                     /locus_tag="ACG8PN_001393"
     CDS             37..867
                     /locus_tag="ACG8PN_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS4861552.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1111..1360)
                     /locus_tag="ACG8PN_001394"
                     /pseudo
     CDS             complement(1111..1360)
                     /locus_tag="ACG8PN_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1551..1745)
                     /locus_tag="ACG8PN_001395"
     CDS             complement(1551..1745)
                     /locus_tag="ACG8PN_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861553.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2250..2435)
                     /locus_tag="ACG8PN_001396"
     CDS             complement(2250..2435)
                     /locus_tag="ACG8PN_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861554.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3285..3626)
                     /locus_tag="ACG8PN_001397"
     CDS             complement(3285..3626)
                     /locus_tag="ACG8PN_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4861555.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3626..3856)
                     /locus_tag="ACG8PN_001398"
     CDS             complement(3626..3856)
                     /locus_tag="ACG8PN_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4861556.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4143..4481
                     /locus_tag="ACG8PN_001399"
     CDS             4143..4481
                     /locus_tag="ACG8PN_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861557.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4686..5248)
                     /locus_tag="ACG8PN_001400"
                     /pseudo
     CDS             complement(4686..5248)
                     /locus_tag="ACG8PN_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(5980..6561)
                     /locus_tag="ACG8PN_001401"
     CDS             complement(5980..6561)
                     /locus_tag="ACG8PN_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4861558.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6744..7370)
                     /locus_tag="ACG8PN_001402"
     CDS             complement(6744..7370)
                     /locus_tag="ACG8PN_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4861559.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7392..8723)
                     /locus_tag="ACG8PN_001403"
     CDS             complement(7392..8723)
                     /locus_tag="ACG8PN_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4861560.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8878..9279)
                     /locus_tag="ACG8PN_001404"
     CDS             complement(8878..9279)
                     /locus_tag="ACG8PN_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4861561.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9365..9571)
                     /locus_tag="ACG8PN_001405"
     CDS             complement(9365..9571)
                     /locus_tag="ACG8PN_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861562.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9666..11168)
                     /locus_tag="ACG8PN_001406"
     CDS             complement(9666..11168)
                     /locus_tag="ACG8PN_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4861563.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11186..11641)
                     /gene="argR"
                     /locus_tag="ACG8PN_001407"
     CDS             complement(11186..11641)
                     /gene="argR"
                     /locus_tag="ACG8PN_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4861564.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11739..12356)
                     /locus_tag="ACG8PN_001408"
     CDS             complement(11739..12356)
                     /locus_tag="ACG8PN_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4861565.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12577..13950
                     /locus_tag="ACG8PN_001409"
     CDS             12577..13950
                     /locus_tag="ACG8PN_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4861566.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14025..15137
                     /locus_tag="ACG8PN_001410"
     CDS             14025..15137
                     /locus_tag="ACG8PN_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4861567.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15209..16846)
                     /locus_tag="ACG8PN_001411"
     CDS             complement(15209..16846)
                     /locus_tag="ACG8PN_001411"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4861568.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17014..17622)
                     /locus_tag="ACG8PN_001412"
     CDS             complement(17014..17622)
                     /locus_tag="ACG8PN_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4861569.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5005 a         3620 c         3106 g         5979 t
ORIGIN      
        1 tgagaatgta ttaaaaatag attaggagga attttgatgt ttagacacca gaaagaattg
       61 cagtttgaag caaaaccaga tcgtccgaat ccattaatcg cgaaatatct gcaggagctg
      121 atcggcggcc aatatggcga gatgagtgta gctatgcagt acctgttcca aggctggagc
      181 tgccgaggag aagaaaaata taaagatatg ctgatggaca ttgcaacaga agaaatcggc
      241 cacgtggaaa tgttggcgac aatggttgct cgtttattag agggtgcgcc tgttgaagat
      301 caagaagcag cgatgcaaaa agatccaaca atcggtgcca ttatctctgg aatgaatcca
      361 cagcacgcga tcgtagatgg cttaggacct cgtcctgctg acagcgtggg caatccatgg
      421 agcggtgcct atatcattgc gagtggcaac ttgttagcag actttagagc taatttaaat
      481 gccgaatcac aaggaagact tcaagtagca cgtatttacg aaatgatcga tgatcgtggc
      541 gtaaaagata tgctgtctgt tctccttgca agagatagct atcatcaaaa tctatgggaa
      601 tgtgcagtga aagaattaga agaaaaagaa ggttcaatct tagtcccaag tactttcccg
      661 cgtgacaaag aacgaaaaga cattgcttat gacttctata acttctcaga gggagaccaa
      721 agtagtgaag gttcttgggc tagtggcaaa gcactggatg gcgaaggcga atatcaatgg
      781 ttgaaagaac ctaagatcga aggcgctgca ccaaaattag aaccagtaac accaaaaatg
      841 tatgggacac ctccattcaa gaaataaata tctacccgta gaaacacccg ttttttgttc
      901 cacacgcttt gtgagcaaaa ctgactaaag tcattaatca taaccaccct tcaaaagggt
      961 ggttttctct agagctataa gctcaaatca ccggccagcg cctaaagacg ctggctttca
     1021 tattttatgt tcaagccata ttgcctttgc cagttttgcc cacctcttaa aaggtttatg
     1081 tactactcct tagcccttaa aaggatcgtc ttactctcgt acactcagct tatctaacgc
     1141 actatcatgt ttttcttgtt cttgaatgta ctttttgatg gtcgcttcat tttgtcctac
     1201 ttttgtcaca tagtatcctt ccacccaaaa gtgtcgatac ccaaatttat attttagatt
     1261 tgcacaatct cttgatgatc tcctgaatat tggctcgata ttgattataa attatttttc
     1321 ttctatactt tggtgtgaat acaatgtggt atttacacat ctactttgta cgggctaaac
     1381 tatttccctt tattgctcca cccgcatagc aagtggtttt gtatttcaat cgctcggcgc
     1441 gctcaattag gtctaaagac aataaatcta aaaacccgct gcttcactga cagaaacaac
     1501 gggttttcag aaaaaaatga aatgaaaaag atctcttacg gcaatctatt ttattttttt
     1561 gattttttat aaatggaaaa tgcttcaaga agtccaagaa ataaagccgt ataaaaacaa
     1621 gaacctatga aatctttatt tattagatat tcaacagaaa tcccgataac actagccaac
     1681 aatatattta tcagaagtgt aaaataagga tgctttttat agaatagaac tagtgttgtt
     1741 ttcatagact ctcctttctt catatttcca attaatcaca aattcacggc attactagaa
     1801 aaattaaagg ggcgcagaag tggaaaaata ctcttcgact ttccctacaa accaggccat
     1861 cttgtgttta ataactatac caaatctaag gtacctctaa atctaaattc catttgtggc
     1921 ggtgacctcc aatttttaac tcttctatct cccatccagt atacataacc ttgatacttc
     1981 gaccacttat atatcgaaca cacaaatacc tatccattgc aggagtacta cctaacttag
     2041 ttgctacttg catctaaata acagctgaaa agataggact tactcttagt aaagtatctg
     2101 aactattaat ttttaaacta tcatttttat cctcaactac atgcacatct gttgcaactt
     2161 cactttctgt ctcatccaga taaatgatta aggaatcggg aattgcaata tcttcagctg
     2221 acgctggaat agttgagcta aattagtcat catatcttta ttataaaata accaacagca
     2281 aaaaatacaa tgaaaaacaa acttggaact aatgtatcat taataaatgt tgaaaaattc
     2341 agcaactcat tactgtatga ttttagcagc ccaaccaaca tataccctat aaaagctaat
     2401 aagatggtta ggaatccttt agaattttta ctcattcata gacacttcct taaataactt
     2461 ttattataga aggtattgta aataataata ttgacaaaaa gtgactcaaa ataaaaaaaa
     2521 ccttacatta tactggaatg aactgtattg atagtgtacc taaaagctct actgacataa
     2581 gatcgtgtaa tcggtaaagt aattttatta attatacggg atgtaatcaa ttagagaata
     2641 aactaatcat aaaaggtact tttacattca taattgtcgc tttgagattc tactttggtt
     2701 cactatctga cccaattgca cattttcgat cattacaagt attggtgtcg ttagaacaac
     2761 taaataaaaa ctagcggtag atttttcaga atctatgcta atttctgtat ggatgttcca
     2821 gtgatggcaa atttctcttt tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta
     2881 ttcagttaaa ctatagaaat tctttcctag acagatcttg atcccaaatt ttacgttttt
     2941 gagcatcgat atagtttaaa atgacattac tcatatctaa agagtttaga ttttttagga
     3001 ctaaaactgt cagttgttct ttttcttctt tctgttaaag tattgtcgcg acttttcgta
     3061 tacgttaaga tatgtcgtga aaaaacaagt tttgtgacac aaaaaaacga atgacttgag
     3121 gaaatcacct caatcattcg tttaaaatgc tatatattct ttagtttaag atacggaaat
     3181 taatgccatc gaccacacta gtagaatgaa cgacatttac ttggcctttt tttaatttcg
     3241 ttatgtttag catataaaaa tattatagag tttacttaca atgcttaaaa catatactgt
     3301 ataatctgat caattttagc catgtcttgc aatggtaagt tttctatttt tttgagtttt
     3361 cgttccttaa aatcaagaga ttttaattgt gtaataatta cttgtccttt tatatctagc
     3421 tcttcaggta atgagtaacg tgtcggtaaa ttctttatag tggaagtaat tggacatatg
     3481 accgaaaaca ttgttttacg gttaaattca taacgagaga cgactagtcc tggtcgtctt
     3541 ttttgaattt cttttcctac ggagggatca aaatcaatcc atacaatatc acctttttta
     3601 ggaatataaa ttctttcttc tgacactata aaatttctct cccttcagga gttaaatctt
     3661 cccattcgtc tttctcataa tactcggcct cttccccacc actaaatgga tcttcaattt
     3721 ttggaactaa cgtaattgtt ccatcatcag aatacacaac gatatattct tggttttctg
     3781 aaggtttctt tccgttatca gaaggtaaag ttaccactac agaacttcct tgtaatctag
     3841 attttgtaac aagcattatc atcactccct taataaaagt atactatata acactaagta
     3901 acgcaaaatt cccctttgta aactatctag aaaattgttt tgtttaaagt atatcttatt
     3961 ggatataaat agaaataaat tgtagccagt tcattttttc tttaactaaa aaaataatcc
     4021 aagaaacatt gaacctatgg atccaaaagc tacccaaata ctatagctaa ccccccgcta
     4081 ccttcaattt tgcagataaa acgctttggg agaaaatttc aaagacaaca ggccatccta
     4141 tcatgcccga ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg
     4201 gaaagttcac gaaactagca ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc
     4261 ttcaaataga aacattggtt caacgaattt caactagaca agactttgct attgtagaac
     4321 gtgagatcgt agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg
     4381 tggataaatg gcaacctctt atctataaat tcagagaata tacaactgcc aaccagtcgt
     4441 taactttgaa ttttagattc caagaattca aagagtcata atgataaaca tcgttaggta
     4501 taaataaaaa agactcgcag cacgagtaaa aaacaaatag aagctataca ttagaaaggt
     4561 cgactgtgac cctatagatg cagtatatgc agcttatgag catttcttct gttctctttg
     4621 tttttgggat ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga
     4681 ttatgttata ccttctccat tactaattca ggcatctgct gagtaatgat ttttcgcgca
     4741 aaagaaatat ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg
     4801 tcatggattg gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt
     4861 ttttcatcct gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa
     4921 aacataaatt tgtctttttc ttcattccac tcattgggaa aacacaacga ttttgcaaaa
     4981 atggttgatg aaaggtcttt ttttcttcta ctgtctctgc acgggcatta atcatcaatt
     5041 gtcctttttt aaatccggta aatccccact tcatgatttc cggagtgagg atgttttctt
     5101 cctcttttct taacgtcaca acaagatttg acggaaaaac ttctccagct gctaattctg
     5161 ctgcctgctt cggttgtttc gtctgggctt ctcgataata attctgaagg agctctgact
     5221 ctagatcaaa aaaataacga ccacacatcc taatccctcc caatcttttt ttctatcata
     5281 cactgcaaca agaagttcgc accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt
     5341 tgtgacaagt gacaaactct ccagtgaatg gtattctcct gccaactcct cggtcacaag
     5401 tcacaatatt caacaaacat gagaagctgc tttttgaaat tgttccttgt gactcggagt
     5461 gaaacggcag gcttacgacc tctgggtaaa cggtgttcaa aagccagttt ctcggtatta
     5521 agccaaattt atgcaaaaat gtagaaagct attttcacaa aatttttctt aatactcgaa
     5581 actaagcgac tttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     5641 aataagccca accaaacgaa aaatatgagc agctattttt atttggctgt tcttattact
     5701 tgatgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca gctaaccttc
     5761 ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata
     5821 ctcaggtcaa agcgctgatt tcacaacctc taatacacgg tgttccagaa gtagcttctt
     5881 cgaaaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
     5941 tttcttgaag ctgaccactt ctgtcacaac ctctaattat taaagaacct tttgcgaagt
     6001 taacaatctt tctttgatca attgaaaatt ctgcctaagt agtgatagat cctgtgtgac
     6061 tttcaacaat aagatacttc cctggatttc agcaatcaac gctttggctt gacggtctgc
     6121 ttcttcatcg gacagttttt cttcagaaag tgccaggata ttttgtttcg tatacttgac
     6181 ccattgattg actaattcta gtagcaacag acggtattct tcatccactg tactcaactc
     6241 aataatcaaa ttacctacag gacagccatg ataagtgatc tgtgtctcat gatcagcgaa
     6301 aatccagtcc aacatttctt ctaatgcttc ttctggctgt tttccagcat ttaagatgtc
     6361 ttcgaacaat tctgtctgcc atacgtccat atgcgcatgg atcaccgcta gacaaacttc
     6421 tttttttgaa gaaaagtaat aataaaattg accttttcct gtatcagaag ctgctagaat
     6481 atctcggatt gacgtttcat gatagccttt tttataaata agatctttgg tcgcttccaa
     6541 gatcttttcc tttgttttca atgatattct cctttttcat ttctcatagt cctatattat
     6601 acagtaaatt catcattttt tcttcttaat cgtttaaaaa agactgtgcc aaattatctg
     6661 acacagccct cactataaaa ctgttctcat atagctagat gttcattgat tcaagaactg
     6721 cgaggacaaa agcaattcct atcttatgat agttgctcta atacttcctc tgttgtcatc
     6781 acttgatctg caataaagcc aaagttcgtc aacgctgcat ccattgcttc ttggccagga
     6841 gcaccaattg catctgtcgc taccacaaca ttgaatccac tctctaccaa ttcacgcata
     6901 tgggaatcgc tacacaagtt tccgttcatt ccaccaagaa ttactgtgtc gattccattt
     6961 tttctcaatt ggagcgccaa atcattgctt tctggtccaa agattttatg aggagagctg
     7021 atgatcgtat tttcatccaa ataaggtttc aattcttcga taaaatcggc tcctgaacct
     7081 tcttccactg attcatattg actttttcta gcaaacatct gctgctcaag catcatatgt
     7141 tctcctgcac cgccaaattg ccatttatgg tcatgtgggt aaaaataatg aggagaaata
     7201 aagagtttgt atcctttttc ttttgcttgt tttaccattt caatcatatt ttcgatcgta
     7261 ttgtattttt ctaagatatc ttttgtcaaa ccaaagccac cgcctgctgg tttcaagaac
     7321 tcgacttgcg ggtcagtgag aactactgct gtatttttat tattgaacat ggtggattct
     7381 ccattcgtta tttattttaa tacatcattt agagcttcac tcattgtcgg atgagaatag
     7441 atttgatctc tcaataacgt ataaggaagt tttccattca tagctagcga aatcaagttg
     7501 atgacttcat gagaatcttc cgcatagata cttgccccta gaatcaagtc agtttcaggg
     7561 tcgactaaaa ctttgaatac acctttagga tcttctaata cttgtgcttt agggacaccg
     7621 cttgtcatat acttgaataa tttgtaattg acgccttgtg ctttggcttg tttttctgtt
     7681 aaacctacat tggataaagt cggagtgata aatacactgt aaggcacagc tttccggtta
     7741 ttcgttgttc gtttattctc gcccttcaac tgatccataa tgattcgata atcatctaga
     7801 gagatataag tgaactgcaa tccgcctttg acgtctccaa tcgcccaaat gttgtctgca
     7861 tctgtacgaa ggtattcatc cacagcgatt gctccgcgat ctgttagttt aacatctgta
     7921 ttttctaatc caagtgatgc tgtacttggt tttcttccag ttgcagccaa aacttttgac
     7981 gcttggattg ttacttcttg gccatcttct gtaaaggtga cggcagctgt tgattcttgg
     8041 tctgctactt gatctacaga tacacctaga tggaattcga ttcccgcatt tgtcatgtct
     8101 tctaaaatca tttgcgcgat atcttcatct tcacgtggaa gaaattcaga atgggcatca
     8161 agaacgacaa ctttagatcc gtattcattg aacattgatg caaattccaa gccaatatat
     8221 ccagccccga tgatcactaa tttttccggt aattgttcct gatccatcgc ttgcgtagaa
     8281 tcaatcaagt agcgacttgt attcaatcct ttgattggta aaatgattgg ttcagaacca
     8341 gtattgatga aaatccgttc gccttcaaca atcacttttt caccattatt cgtcacttcg
     8401 atttgatggt tagaaagaaa acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg
     8461 ctgatcatat gataattttt ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca
     8521 gcctctgtaa agtcgattcc tctttctcca ttgaaaatca atgatttaga aggaatacag
     8581 ccgatgttga tacatgttcc gccatacata cgtgttgact tttcaacgac taaaaccgat
     8641 tcaccttttg aagctaacgc cttcgctaaa gtcttaccgc cctttccaaa accgatcaca
     8701 atattttgat atttttccat catttttccc tcgcttcttt tttacctacc gattggtaca
     8761 aaaatatgat aaccgataat tgtctattct ccaaatcatc tgctttattt gtcaaaaaaa
     8821 accagacaaa aagcggtcct ttcacgtttt tagtctggtt cgatttcctt tattcttcta
     8881 aacaatcgtt acttctacat cgatatttcc tctagttgct ttggagtaag ggcagaattc
     8941 atgcgcatct tctgcaattt tttgggcttc ttcttcgctg atgccatcga tatgcacttc
     9001 taatactaca ccgatctgat agccgccatc atctaaatta taaagagaga cacgtgcttt
     9061 tactgtggac tctgctgtca ttcctgcttc tttgatcata tgttgtaccg ccccattgaa
     9121 acaagaagca taacctgctg caaataattg ttctggattt gttccttctg catggattcc
     9181 tggaggagtg attttcatat gcatcgagcc atttggtgcc tcgacttctc cattgcgccc
     9241 gccatggttg atcattgctg tttcgtatgc ttttttcatt gtactgctcc tttcgattga
     9301 ccatttgcta cttggctata attatagtgt atgacaagta aggcgctttc tacaaaaatt
     9361 ctgcttattc gaataccaga atgccgtctt ctactatttt tccaaaaaac aaaaaaatac
     9421 tactcgtgaa caacagcgtc atatttgttc caaaataaat agcaatcttt tctacataat
     9481 cttttatatg attgatttta agatacatat ataccagaaa gttgacccca taaaccaata
     9541 ctgttaaaaa aataaccata ttcgttacca ttttttcctc cttttgctgg tctccctacc
     9601 agcactcgat ttaaccatac cacttttttt gccgtccaag caactagcct ttcaaaaaga
     9661 ctggtttatt ctgctgttaa tgattcaaga tcagaaaaat gtccgaataa ttttgctaat
     9721 tttactaacg ccaacttgtt ctttccgaat tcatcatttt ctatctgtaa tactagtaac
     9781 ggctcatgta agcttaaacg gatcaaaaac cagccgtttc cgtatggctc ttctagatca
     9841 tatcgaatac cttcttcatt ttccgggtcg aagttcatcc ctttttcttc ttccaacagt
     9901 cgcttcattt cttctaatat cttgtttcct tgttcgcgtg gttcatcgct gaacaatttt
     9961 aatcggattt cttgtacttc tacgggctgt ttcaactctt tgatttgttc atctaatgtg
    10021 ataccttttc tttttaattc tggcaaaagc atcaggatct ttgcgatgac atatgcacca
    10081 tcatccagaa aatagttttc cttgaatgca gcgtgtccgc ttgtttcgat ggctagctgt
    10141 gtatctttcc caagacggtt aagttctaat gctttattga ttacatttcg atagccggaa
    10201 atataacgaa cttggatgcc tcctttttct tcaatgaatc tttgtagatg agaagaagta
    10261 ggggaattcg tcacgattct tgttcctgga tgctctttta atacgattgt acttaatact
    10321 gcgatcaaat tatttcgatt gatcactgtt ccagaagcac tgacaactgc agaacgatcc
    10381 acatctgtat cgaaaatgat cccaagatct gcttcatttg ctaaaacagc ctgcttgata
    10441 ctttccatcg cttctttatt atcaggattt gggatatgat taggaaatcg gccatctggt
    10501 tccaaaaatt gggaacctgt tgaatccgca cctaagctat tcaatacttt ttcagcaaag
    10561 aatccaccag caccatttcc ggcatcaaca atgattttcc aaccatttag tggtttgctt
    10621 tctagtgttc caatcccctt tttaattttt gtgactaaat cttgcgcata aggtgtcaaa
    10681 aggtctgctt tttctacgct tccagtttgt tcgcttttta ttttttgctg ctgtgatagg
    10741 ataaaagcaa tatcttcttt ttctgctcct ccttctgagg taaagaattt tattccatta
    10801 taataatagg gaagatggct agcagtcagc ataaccgcag catcgcacga gaactgttca
    10861 aattgagtgg acataaacat agcaggcgta gtagctaatt cgaaatcgat gacatgtatc
    10921 ccttgttctt tcagactttg gatcaacgcc tctttcagtg ctggtccgct tatcctactg
    10981 tcatgaccaa tcccaatcgt ccatttttta tttggatcgg ttgatttctt ctttttcttt
    11041 atccagtttg ctagtcccca gccgatcttt tttaccatgt ctgtcgtcaa gtttacttca
    11101 tatccttcaa ctggtaatgc gattcctcga atatccgatc cattttgcag cgctttcata
    11161 tctttcatat aaaagtcctc ctcatttatt ccctcatttt ttccagcttt tctgcaaatc
    11221 gctgtgcttc ttcttctgag cggcaaataa taatgaatgt atctacaccg gccatcgttc
    11281 cgacaacttc agggtaagcc acttcatcta gccaattcgt taccacatcg gcaccatttc
    11341 gttcagtcaa tacgataacc atgaactgaa cagactggat acgcaaaacc tcgcgtctaa
    11401 ttgctgtacg caaacgttct tcatttaaag aaacagatgg ctggttaaat aatgtatagc
    11461 gtactatttt gttttcattt tgacttttta ctaacttcat ttctcgaatg tctcgagaaa
    11521 tcgttgcttg agtcacatcg gcgcctgctt catgcagccg cgcaagcagc tcttcctgtg
    11581 tcccaatagg ttgttctgta atgatctggc ggatcagtgc ttgtcgctgt ttctttttca
    11641 tattagacac ctctcaatgt tgattatagc aaaagataag taatatttct tcatattttg
    11701 gtatttttta cttttatttt ttgttttcat tcaggttgct aacctttttc tattttaaca
    11761 cgcaaaatcg tcttcatttt ttcaggtgct gattttcttg gatcgctgat gtaaatctct
    11821 ttgtgttctt tagaagctct ttcatagcct tcttttgcga tgaatgcttc taatttctca
    11881 aacgttgctg gttcttcggc atagggcccg acgtgtaaga tttgggcaca aagaccttct
    11941 tccatctttt tgaatactac ttgttctttc aagtcttccg gaagtttgct agaacgctga
    12001 atggcttctt gtgctatcgc ttctgtaaca aagtcaggct gtcggatcat caattgataa
    12061 gaaaaatgtt cttttttcat cactggttca tgcaaatact tttcttgcag tccccattgt
    12121 ccttccagtg gatagacagt atacatctga taatcaggaa tcagccaatt ttttttaggc
    12181 gacatgcgta ttgcatagct gactgcatat agacaggcta ctcttcgttt aaagtcttcc
    12241 tgattcgggt cgccgatccc tctgatgcta aagaatgatt ttttaggaac ctctagtatt
    12301 tgcgggcttt ttttcatact atagctcttg tctttacgcc attcaatttt ttccattttt
    12361 cttcctccgc gaaattccat ttagttattt attatcatac ctctttttga gtgacttggc
    12421 taatcgtgtt gacactattt ttcagtttgt gctagaataa gtgaagtaaa taaatcgtac
    12481 agataacacg tttcggcgtg tcgagagaga ggcggtttct atttttataa tagaaatcac
    12541 tctctttttt tacgaaattt tttagaggtg atgtaaatga caaaacaaaa acagctgctt
    12601 ttagtacctg cagtaattgc aacagggatg atgtcttttg cgggcgtatt gatcgagacg
    12661 gccatgaatg taactttccc aactcttatc agacagtttg atttaacgac tgcacaagtc
    12721 caatgggtaa caacgattta tcttttgatg atttcgatca ttgttccgct gtctacttat
    12781 ttgaatcgca attttagtct caaaaaactc tttttagctt caaatctgct tttcttggca
    12841 ggtataatga tcgatttctt ctctcctacc ttcatggtcc tattgtttgg tcgattgctc
    12901 caaggtgcag cgacaggtat cgctcttccg ttgatgttcc atattatctt gacatttgcc
    12961 cccctcaatc gacgaggcgc gatgatgggt gtcggtactt tgacaacggc cattgctcca
    13021 gcaattggcc ccacttatgg aggcattatg acttcacatt ttacatggaa tcatatcttc
    13081 ctgtttcttg tgcccgtttt gtttctttct ttgattatcg gctggtttgc tattcccgag
    13141 atggcagtac aaaaaagtgg ttatttggat tggataagcg ttttaggaat ttgtcttttg
    13201 tttagtggaa gtctaacatt tttgagtacg ttgggaacag tttccagctg gattgcttta
    13261 atcatcggcg gtattggctg gttccttttt tactatcgaa gtaaaaaagt ggagcatcca
    13321 ctgatccgac tgtctattct ttcaaataaa acattccgct tgtttttgat tggcttctta
    13381 gtttatcagt ttttattact tggtgtatct tttgtcttgc cgaatttcct tcagatcgtt
    13441 caaggaagta gtccttttgt cgctggatta gcgatgcttc ccggcgcagc ggttggtgct
    13501 ttgctttctc ctttttccgg gaaaatgctg gatcaatatg gtccgaagaa gccaattttt
    13561 gctgggctga ttctttcttt gattggttgg ctagcattat tcctactcat aggacacgct
    13621 tcagtgggtg tattgattgc ttgtcatgtg ttttatatga tcggtatcgg attatcttac
    13681 agcaatatga tgaccaccgg aatgaatgca ctgaatgaag aactacaagg agacgggaac
    13741 gcaatgttca ataccttgca gcagttttct ggcgctgtgg ctacttcatt agtagcggtc
    13801 attatcaatt acgtccagca tcatacttcc cataactatg aagtgtctac tacattagga
    13861 tcaaaagcag cattaggcgt cttgttgctg ttgcttctcg taagctttgc tcgattcgcc
    13921 tattatctgt tctttgctaa aaaagcgtag attccatata cggcaggtgt tgcatctgcc
    13981 gtttttttgt gtgaaaatgg ttgtttgtaa aaggaggctt ttttatggaa aaaattgcca
    14041 ttatcggtgg cgggatcatt ggtatgaccc tagccagtta cttagataca tcaaaattca
    14101 atgttgcttt atttgatgaa gggacaggac aggcaactaa agctagcgct gggattatct
    14161 ccccttggtt gtcgaaacgc agaaacaaac aatggtatcg tctggctaaa gacggcgctg
    14221 cttttttccc taaacttata aaagatttcc aacttgatca aacgatttac gcacaatcag
    14281 ggacactttt gcttcgacct gaaaaagatt tagctgaatt ggctgttctt gccgagaaac
    14341 gaaaagaaga agcgccagaa attggaaaca tcgaatggct ttcacctgaa cagacatctc
    14401 aagcacttcc gttattgaaa ccagcagttt cactgcgtat cagtggtggt ggtcgtctgg
    14461 atggaaaagc atatttagaa catcttggtc agcgtttagc acaaaaacaa atggagcaga
    14521 tccaggcaaa agtcactttg cataagaaga gcgattcttg gctcatcaag ggaccgtttg
    14581 gagaatacac agctgatcgt gtaattttga cacctggccc ggcacttaag cctttattac
    14641 aagaattagg ctatgctgca gatatacgtc cgcaaaaagg gcagctctta gcatttcaga
    14701 catcttataa gatgagtcaa gactggcctg ttgctatgct tgacggagaa gctgatttca
    14761 ttcctttcat ggatggaaag atccttttag gtgctaccca tgaaaacgat caaggctggg
    14821 atttggctcc tactagcgaa gcttataagg gattgactaa gagtacagaa cagttcttga
    14881 ctgatccaga tcagataaaa aactggccat acgagttccg tgtaggtacc cgtgcttata
    14941 cgtcagattt ttccccattt ttcggcgtac tgccagatga tccaagctta ctggctgcta
    15001 gcggtctagg ctcttctggc ttgaccacag gtccttatat cggctatcta ttagccaact
    15061 tcttaaatac tggtcattgg cctacggaag attatcaaaa ggacatctcc acctatatta
    15121 cgaaaaatcc atcatagaaa aacagctttt tcaagtaagg atcccgagcg atattgcgca
    15181 tggattactt cttgaaaaag ctgttatttt agttcaattt ctgaatcaaa ttctccaaac
    15241 tttcttttgc ttcatctggc aagagatctt ttcctatctg agcttcaatg aatcgtaaac
    15301 gaatatcaag tcgttgtttc accagatttt tataggacgt atcctcaaaa gaaacaggaa
    15361 aatctggtat aggtaaatag tggatgtctt caaatggacc aaacgacttg aattctgccg
    15421 tttgttgcac gattctttca acgattgaca atgtcgtttc aggtttgatt tttttatcat
    15481 cgaaatatcc agtattcagg acatagcagg ctgtttgctg tttcaaaaac aactctcgaa
    15541 aagcttgata gtcttcccta agaggataga cacggaaagg attagcaaat ggttcaatga
    15601 ctagctcatc tagctgtctt tttgtagtga tattttccgc gtctgatcgt tttgttgcta
    15661 atgttgcacc aaaagacgct gctaaaactg ggtcattgat cttgataatt ggcggaagcg
    15721 aatcatcttt catgatccaa aaaacagcat cgatcttttc gctaagatga tcgactcggt
    15781 cgggtgtcgc ataacgtgat ttgactgtcc gcccatttcc gtttcgtata tctcctgtta
    15841 ctaggacttt ttctccttta cttgtacatg tcacgcccac attctgacaa gtaaggaaag
    15901 aagaaatggc cggatcatca gacggatagt cttgtgtttt atcaaagtaa gaaggttcca
    15961 aggcagtagc aaaaccattt tttcgagaaa taacgaaagc atcgtcatgc aagatcgaaa
    16021 cagagtgacc ttccgtatgt tttgctaagg taatcgttga ttttccagaa ccggacaacc
    16081 caaatacagc catcgtatag gtctggtcat tactagcata ttgtttcatc ccaccgtggc
    16141 aggcaataaa gccattgcga tttcccaatg cccaagcaag cgtcaaagtc gcttttttta
    16201 attctccaaa gtaacgtaat cctagcacag ccgctgtatt atggagagca tcaaacaaaa
    16261 tgagtccatt tggaaatgtt tcatgtgtcc attcgggatc acaaaagata taaagatctc
    16321 cctcatcaaa ggaaacagac ttttgataag tctctaagat cttcgaattc gctggctgaa
    16381 aattcagcaa ataagaatac aaattgttct caaatccttc tggaatcagc aaatgacttt
    16441 gcacagaaaa ttcttcagat aggccaacga ttacttttgt atggtaaaac tggcgcttgc
    16501 gactttgata cacagcttct cttaaaattt tctcgtattt gctttgttcc ttgtctaagt
    16561 cctcaaagat ccgccgagca cttgctgttc ttcccacaat gcttccatca ttgaatacta
    16621 gtgtcctaac atcagctggt aaatctagtt cttcagcatg tgagattgtt aaatctgtga
    16681 tgatcgttcc tggactccct tttgctagtt gataagctgt cttcctatca aaaacttcat
    16741 gaacattatt accgtaaaat gtcgtttcta caatcgatcg aatactggat aacagtggat
    16801 ttccacttgt gatttcgttt aattcgagac tctcttttgt actcatggct cctcctcatt
    16861 ccctaaacaa aaaaacgttt tctttattat acattattcg agtttttatt tgtactgctg
    16921 cgctgtggga agcaaacgag ttatttttca cacaaaaaaa ccgagaactt gaataaacaa
    16981 gtttctcggt ttcatcattt tccttttatc ggcttagtac cagccgtttg ctaaccagaa
    17041 tgttttagca gcatcccatg aaccataacg agatgttaca tattgttctg ctacacgttc
    17101 ttgattggca gctgaatagt cgccgtttaa gtatgaagca tctaattgat aacggccgat
    17161 gtaacgtccg ttagtagctg tgtatgaacc gcctgattct ttttgtgcga tccattcttt
    17221 tgcagagctt gaagttggcg ctgttgcagc agctggggca gcaggagcta cttcaactgt
    17281 ttcaacaggt gcaacgtatg tatgttcttg cgttgctgtt tcagttgtag atgctgtatt
    17341 ttctgttgtt ttgttttctg ttgttgctga agaatcattt gaagttggga ttgttaattg
    17401 ttcaccaaca taaatgcggt tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat
    17461 cattgagttg tctcctgcaa atttttgtga gatcttagat aatgaatcac ctgattttac
    17521 tgtataaact tcatcagcgt gagcatttgt agttcctaca aacattgcag tagcaccagc
    17581 agcaaaagtt gtacctaaaa caattttctt aaatgaattc atattaaact cctttttttc
    17641 tccgtttttt attctttcga cgctattaac tttatcattt agaaatattt cataggtaaa
    17701 agaaacatga
//
LOCUS       DAZUKA010000053        17651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000053 DAZUKA010000000
VERSION     DAZUKA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.053"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..421
                     /locus_tag="ACG8PN_001413"
     CDS             191..421
                     /locus_tag="ACG8PN_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4861570.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACG8PN_001414"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACG8PN_001414"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4861571.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACG8PN_001415"
     CDS             1832..2008
                     /locus_tag="ACG8PN_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4861572.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACG8PN_001416"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACG8PN_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4861573.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="ACG8PN_001417"
     CDS             2624..3091
                     /locus_tag="ACG8PN_001417"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4861574.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="ACG8PN_001418"
     CDS             3176..4060
                     /locus_tag="ACG8PN_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4861575.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="ACG8PN_001419"
     CDS             4174..4410
                     /locus_tag="ACG8PN_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4861576.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="ACG8PN_001420"
     tRNA            4498..4578
                     /locus_tag="ACG8PN_001420"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="ACG8PN_001421"
     tRNA            4585..4656
                     /locus_tag="ACG8PN_001421"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="ACG8PN_001422"
     CDS             4781..4951
                     /locus_tag="ACG8PN_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861577.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="ACG8PN_001423"
     CDS             5171..5878
                     /locus_tag="ACG8PN_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4861578.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="ACG8PN_001424"
     CDS             complement(5937..6581)
                     /locus_tag="ACG8PN_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4861579.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="ACG8PN_001425"
     CDS             6707..6994
                     /locus_tag="ACG8PN_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861580.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="ACG8PN_001426"
     CDS             7102..7422
                     /locus_tag="ACG8PN_001426"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4861581.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="ACG8PN_001427"
     CDS             7503..8816
                     /locus_tag="ACG8PN_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4861582.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="ACG8PN_001428"
     CDS             8813..9532
                     /locus_tag="ACG8PN_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4861583.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="ACG8PN_001429"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="ACG8PN_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4861584.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="ACG8PN_001430"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="ACG8PN_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4861585.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="ACG8PN_001431"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="ACG8PN_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4861586.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="ACG8PN_001432"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="ACG8PN_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4861587.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14978..15862
                     /gene="ftsX"
                     /locus_tag="ACG8PN_001433"
     CDS             14978..15862
                     /gene="ftsX"
                     /locus_tag="ACG8PN_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4861588.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="ACG8PN_001434"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="ACG8PN_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4861589.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5900 a         2979 c         3831 g         4941 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact
    17581 ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag
    17641 agacacattt c
//
LOCUS       DAZUKA010000054        17443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000054 DAZUKA010000000
VERSION     DAZUKA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.054"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..752)
                     /locus_tag="ACG8PN_001435"
     CDS             complement(207..752)
                     /locus_tag="ACG8PN_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4861590.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(764..1456)
                     /locus_tag="ACG8PN_001436"
     CDS             complement(764..1456)
                     /locus_tag="ACG8PN_001436"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4861591.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1475..1759)
                     /gene="macP"
                     /locus_tag="ACG8PN_001437"
     CDS             complement(1475..1759)
                     /gene="macP"
                     /locus_tag="ACG8PN_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4861592.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1772..2335)
                     /locus_tag="ACG8PN_001438"
     CDS             complement(1772..2335)
                     /locus_tag="ACG8PN_001438"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4861593.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2563..3225
                     /locus_tag="ACG8PN_001439"
     CDS             2563..3225
                     /locus_tag="ACG8PN_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4861594.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3349..4545
                     /locus_tag="ACG8PN_001440"
     CDS             3349..4545
                     /locus_tag="ACG8PN_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4861595.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4646..6022)
                     /locus_tag="ACG8PN_001441"
     CDS             complement(4646..6022)
                     /locus_tag="ACG8PN_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4861596.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6059..6926)
                     /locus_tag="ACG8PN_001442"
                     /pseudo
     CDS             complement(6059..6926)
                     /locus_tag="ACG8PN_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(7015..8040)
                     /locus_tag="ACG8PN_001443"
     CDS             complement(7015..8040)
                     /locus_tag="ACG8PN_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4861597.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8058..8732)
                     /locus_tag="ACG8PN_001444"
     CDS             complement(8058..8732)
                     /locus_tag="ACG8PN_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4861598.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8916..9809)
                     /locus_tag="ACG8PN_001445"
     CDS             complement(8916..9809)
                     /locus_tag="ACG8PN_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4861599.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9883..11580)
                     /locus_tag="ACG8PN_001446"
     CDS             complement(9883..11580)
                     /locus_tag="ACG8PN_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4861600.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11573..12886)
                     /locus_tag="ACG8PN_001447"
     CDS             complement(11573..12886)
                     /locus_tag="ACG8PN_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4861601.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12886..16827)
                     /locus_tag="ACG8PN_001448"
     CDS             complement(12886..16827)
                     /locus_tag="ACG8PN_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4861602.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16936..17394)
                     /locus_tag="ACG8PN_001449"
     CDS             complement(16936..17394)
                     /locus_tag="ACG8PN_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4861603.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4912 a         3899 c         3030 g         5602 t
ORIGIN      
        1 gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat ataacttaag
       61 ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa aataacgtat
      121 aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat tttgtgcttt
      181 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggactcaaaa
      241 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggaatc tttgaaaata
      301 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
      361 catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat gtctagatct
      421 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
      481 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
      541 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
      601 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
      661 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
      721 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
      781 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
      841 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
      901 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
      961 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
     1021 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
     1081 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct gggttgtagc
     1141 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
     1201 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
     1261 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
     1321 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
     1381 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
     1441 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
     1501 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
     1561 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
     1621 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
     1681 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
     1741 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
     1801 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
     1861 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
     1921 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt gcaaaacctg
     1981 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
     2041 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
     2101 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca agaagcagac
     2161 gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc agttctctct
     2221 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
     2281 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
     2341 ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt tctcaaaaac
     2401 tccttattag tttaccatag ataaacctta gattttctct aaatatagaa aatccttctt
     2461 tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc gaataacgaa
     2521 aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa acgaaattgg
     2581 ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag tttttctttt
     2641 gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt tggttcgcgc
     2701 aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga aactcactat
     2761 gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat gaatcaaaca
     2821 aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact taaagcaatc
     2881 gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa
     2941 ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa tctagttgat
     3001 ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca gacctatgaa
     3061 aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc acaagattat
     3121 caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt tgcgaaacag
     3181 agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt ttgttcataa
     3241 aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt ctaaagtaga
     3301 gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat ggatcataaa
     3361 ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt attgaacaac
     3421 ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat tgatcagctc
     3481 caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg ccaagaacaa
     3541 gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat cagctacgga
     3601 tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca tgtgcaagca
     3661 caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct ccaagaagcc
     3721 aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc
     3781 aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag
     3841 atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct gatgttagaa
     3901 cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat cgctgctgga
     3961 gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa aagagcagaa
     4021 gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt cctagatcgt
     4081 ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca gcaagcacca
     4141 caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca agcatctatc
     4201 gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa
     4261 aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt attgaagaaa
     4321 aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga acgtggaatc
     4381 gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat ccaagagaca
     4441 ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac
     4501 atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac ctttcgcttg
     4561 ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt ttttcttatc
     4621 tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt ccatagccat
     4681 agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc catgtcacag
     4741 tttgcagacg aagcatcaat acaatacaag tcaaaatgga gacgattggc aatagcggaa
     4801 cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc aacttcagta
     4861 agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt tccgctaata
     4921 cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca taagtcgctt
     4981 ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt aagccatcct
     5041 tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt accgtcagta
     5101 gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct acatacctca
     5161 ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt cctgtcaaga
     5221 tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg attcctcttg
     5281 gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca tcaaatccca
     5341 agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca aaaggcatga
     5401 atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac aacgcgatga
     5461 taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg gcttcaagcg
     5521 acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg tatgtaccat
     5581 tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa
     5641 aaccttgcat atagccagac catccggaag caacagagga aaccgctagt aaaaattccc
     5701 cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca tacagatagg
     5761 catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt agtccagaca
     5821 aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca gcattttgat
     5881 tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca attccaagca
     5941 tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg agattgatct
     6001 ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa aacagcgatt
     6061 aaactgtttt catataccct aaaagggata ccacctaaaa tgactaaaag gcaaccaaca
     6121 ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa
     6181 gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg
     6241 actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat catgtttttc
     6301 cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc taagatcatt
     6361 ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt gacgatgatg
     6421 gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt accaaaatca
     6481 agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt caaatacgca
     6541 ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt ttgtgtccat
     6601 tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc agtagacaat
     6661 ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt ttgtccaaca
     6721 ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat tacaggacta
     6781 acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt
     6841 tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca tgccaacata
     6901 gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat gataagaaac
     6961 ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga ttttctaacg
     7021 tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc cttttccttc
     7081 agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg ctaaataaag
     7141 tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa atcccttatc
     7201 aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa ctgaaaggct
     7261 gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg gcgtatattt
     7321 gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat cggtcaaaac
     7381 agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat aatttgcctg
     7441 actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga ggtagtcccg
     7501 cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat cgatttttga
     7561 taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga tcgaacgaac
     7621 tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt caatataatc
     7681 tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt cacggatcag
     7741 ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac tcaaataatt
     7801 ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc tcttgctcaa
     7861 gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta aataagcaat
     7921 tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg tcaatgcaat
     7981 aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa aaaaattcat
     8041 cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt ttcgatatat
     8101 ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta
     8161 ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc ccgacttaag
     8221 atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt gctcaaatca
     8281 atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat gccattgtgg
     8341 ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt
     8401 tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc
     8461 atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat cggtactttc
     8521 gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag attaatatct
     8581 agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt gaaatcggtc
     8641 gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg tcgaatgatc
     8701 tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt tcttcttgct
     8761 actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa tgtcatttta
     8821 gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct tccaagcagc
     8881 ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt ctttcgttgt
     8941 ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac tagcaatata
     9001 cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc tatctttgaa
     9061 gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg agtgttcacg
     9121 gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat tgctaatgta
     9181 tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag catccgacca
     9241 tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt aaagagtttt
     9301 ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc tccagttgga
     9361 aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt cgtttcatca
     9421 aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat atgttgttcg
     9481 taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg atctttgact
     9541 tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt ttcatgcact
     9601 gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg ataattctcg
     9661 atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata tgtaaaaact
     9721 gcagctccgg caagaaacat cactgccata aataattgta ataaaagact gatttgggtt
     9781 tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa aaaaactgca
     9841 gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt cagacttcat
     9901 tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga ttagtaagaa
     9961 tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat tcggtactct
    10021 ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc ttccataaga
    10081 gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt ctggcgccat
    10141 ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt atccatttcc
    10201 atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat ctgctcctga
    10261 ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag aacggtcttc
    10321 attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat catatttttc
    10381 aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt cataatcaaa
    10441 ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat ccgcttctag
    10501 ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg catctgctaa
    10561 tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg tcacgacaaa
    10621 cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg tctcgccggc
    10681 tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat ctgtgatttt
    10741 gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat aaggaatagg
    10801 tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag aaatcgtttc
    10861 ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct ctacattcat
    10921 ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg tattgtatgt
    10981 gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca tcactggcat
    11041 cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag tttctaagaa
    11101 aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg tagagaatac
    11161 cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa
    11221 ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat taagaccgtc
    11281 ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt ccgttacgtc
    11341 aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag aaaccaatcc
    11401 gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg taatcgtcac
    11461 ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc cgaaaatccc
    11521 tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt ttttattcat
    11581 ttttttctcc ccgttctttt tttattaata aaacagcacc taacaataaa agaattccta
    11641 gtaaagaaat caacgttctt gcttcattcg tttgcgggaa tacggaccat tttcctgatt
    11701 gacctggatt gcttggacca ctcggctctt tcggtttgtc cgttgtttct tttacggtct
    11761 cgttataaac acgtggtgtt gcggattcat aggcagagac tggaactttc ccggattcta
    11821 attcagccat ctcctgtccg ttaactacta catattgtgg ttggcctttt gcatccaccc
    11881 aaaattcagg gatgatgacc tcgattctct tacttgcttc atccatctcg taaccttcca
    11941 caccttttaa ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa
    12001 caaggccctc tttatccgaa atgaatttgc ttacattttt gtcattcaaa ggatcattgt
    12061 ctgctggttt gatccattga tttttcaagt cattagcagg agccatatct aaataatatt
    12121 tccccccttc tatcatctga tatagagcaa aaacagcacc ttctaatggt tttccttttt
    12181 cagttgttcc tctttgacgt ccatacttga agaagtatgg atctcttaga taaccgacat
    12241 ttttaggata gatatgaatc tcttgcagtt cctcggttga ttctgtcggg tgataaattg
    12301 gcaaaatagc agcaattggg atagctgtct gatcgatatc aacatccaat ccaacttctg
    12361 gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt ttcttaggtg
    12421 ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt atctttccca gttgttaccg
    12481 aagtccctat ttggacaagc ctttcttctg tgattagctg acggatattg tttctgttcg
    12541 tttcagcgat ttgctggacg aattctttag gagtcatgtt ttcagcagct tttttttcat
    12601 gataataatc ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc accgtctgtg
    12661 aaatgatatc tgctgtttcc cctgcttcct cagagatagc caagccatca ttttcatggt
    12721 attcaaattg atcgttataa tcgatatctc gaatcatccg tttatgcaac acgaattcca
    12781 cgacatcttc ccctgattct tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg
    12841 gtagtaaaac aagtaattga agcatcaaac acaacagctt tttcattacc ttgctccctc
    12901 ctttttagat tgtctgacaa acaaaccata ggctgctcct gcaagcgcca tgaagaatgc
    12961 agcaagcagg atatagaata atcgtccgga tcctcctgta gcaggcaacg gattttcagg
    13021 atcgttcccg acactaagct gtaatctgat tcgatttgtc tccaaaagtt catattctaa
    13081 ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga taatacgaaa tctcaacttt
    13141 tagtgtaccc gcttcttttc ctggaactac ccgatcattt tctatgtcag gttcttctcg
    13201 ataactaatt tctaaaataa agtaacctgg aagttttcta tagctgtcag gtgacttcac
    13261 ttccttgatc cgatagaccc cttccaacaa gtctgaagca ttgaataggg aagaatcgtt
    13321 tggatctggt gccaagcctc cctcgatgat ttcatactct cctgcactat tttgtttctc
    13381 aattgaaaat tctgcgcctt caagacgtcg gctattcata ctgtcaattt tcaaaagttc
    13441 caaatcgatt ggcgctagtt catcttctaa ggttacttgt agtacaccat cgatgatttc
    13501 atagccttcc ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt
    13561 accttcttca ttcagtgcaa actggaacac ttgatcagat agccgatacc catttggtgc
    13621 gacagattcc gagacctcat attcaccaat agacagatgc agaggttggt cgtaatttaa
    13681 atcgacatac ttttctactc ctacggcacg agaatagaag tatccgatcc caggcaaacg
    13741 tttcgattct tctggttcta acggattacc agtgtatgct cgatacagcg ggaaggaaga
    13801 atcccccact ttcctgatat cgaaacctgc accatctaat gtatggcctt catggtctaa
    13861 ttttctaact cggatctccc gataatcaaa cgtattgtaa gcagtgaatg ctacccataa
    13921 acgatcctct tcatctgttg tatacgtgag atattgccaa ttttcttcac ctgtttgctt
    13981 gagttggata gtccctatag cattatcgta aagtcgcaag atccatgtgt cattttcatc
    14041 aggttctttg aaaccttctg ggttctggct ctcaatgatt ttgtattctg catagttggt
    14101 agtttcttca ctatccttat aaacaaatga atctggctct ccgattggat gaataacttt
    14161 ctcaccatcc acgattacat atttcccgtc ttcatctact gaatagaacg tgagtctccc
    14221 tttctcttcc tctacttcgt tcacaccaga aagccctttc gctactggat atccgatttc
    14281 cagtccgtta ccatctgttt cataaagttc aaactctgcg ccattgattg gcatattact
    14341 gccttcaatt agtttattga tattcatgtg gattggcttg aattcatttc gcatttccag
    14401 tttgacatac aactcatcat actctgtgcc ttctatgtta ggctcttgta caacagaaag
    14461 cttgtgatat tctccttcat aataggaatt cggatcttta ccttccggtc cgccgactct
    14521 cgaaccagat gtatattcat ctacaagctc gtagtgacct aacatctcat cttcgttggc
    14581 tgcatatttt ttgatttgga attgatacgt ctcatctact aactgataac gtggtggtcc
    14641 agcgatctct ctgatccaaa atatgtcgct gactgctcca gtttcatctc ctaaaatcgg
    14701 catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt cgattggttc
    14761 ggcttcccca ctctctggat tttggtcata taatgcaaat tcgactccag ccaacggctc
    14821 gccattttga cccttcttgc tgacttggaa attacggcct ttatgttcgt aacgaatgga
    14881 agaagatggg aaatacatcc gtccagtagt gatatcattc tcataatcct ccaggttctg
    14941 aatataggcc atattgttca ctgtgcaaaa atttccgtct tgagctcccg catcaagttt
    15001 tacttggtac ttgatcctga ccatttcccc ttcacctaaa gaaatcggag aaaatttata
    15061 agcttggttt tcccgtccga catacgttgt cggagtcgcc actacttctg gtggggcttg
    15121 tccatgacgt tcttcatccc atatttctgg ctcttggtac ttcatgccgt tttctcctgg
    15181 atctagatgg taaaactgaa tatctgccga tctgccagct ccaaatggtg agacattaaa
    15241 accagtttca tcataaagtg tggcttgctg gatcgttctc ttgaaggtag aagagatatc
    15301 ctccaaggct gtaacaatgt cattcttgtt gttcgcattg taatatcgcg gtgttacgcc
    15361 atcttcttct aaatcagcaa tatttttcaa tacatttcgt ccaatagcat ctagtcgttg
    15421 tctcccgcta acgtttccat cgattcctaa accaatggag aagatacttg gcgggttctc
    15481 ttgatacttg tttttcagcc aatgatgata agcaactgtg ttgatcgcag atgacttttc
    15541 ttgcgaagta ggactgccat tcgtattgtt attagaagaa ccatcaccaa cccagtgagt
    15601 cgctttatta tctccttctt ttacctctcc ataacgatag tgtacattta cttcatcttc
    15661 tggacggtta aagtcagatg cataggtaac tggatgagta aaccctgcat tagaagtatt
    15721 cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc
    15781 catcacagtc cagttgctcc aagctccatt agtgctcgta cgataacttg actgaatcgg
    15841 tgcataactg aaggtcggtg tcccatctcc cacaacgatt aggatttttt ctggatttct
    15901 attttctccg ccattgtctt tatacaatgt ttcatagcca tttttaagcc ctagtgtaag
    15961 cggagttcct ccgattggtg tacgatttaa tgctgtattc tcacttcccg tcaaacgatt
    16021 gatatcattt gtcaatggaa agatgtccga actgatttgc tcatggttat tgatagctgt
    16081 actattatgg taaaaattca ccataccgat tcgaatatcc agactcgggt cagagagcag
    16141 atctttactc atttcgttta ctgcctcaat caacgcagca tttttagttt gactattggt
    16201 cccagcggtt aattcactca tactcgctga cttatctaga acaaaaacga tatcgattgg
    16261 actgatttcc tttttctcgc ccccgatgac atctagatag acattgtaaa gggttgttgc
    16321 atcttcagga tgtagatcag atgctgtgct gcttggcaga acaggttcta cccatttttt
    16381 gatgtacgcc ccgttataat ccaagtaact gttttcaaaa ttccgttcat ttccccaaag
    16441 ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct gcagttgatc cattattagt
    16501 agcatagtcg taatttcgcc agttcgctcc cccgtttgta taatcactat catcgatcaa
    16561 gttctctccg tcatatggcg agcctttgtc tccttgatag cgttcattgc tttgtgtcca
    16621 atttcctaac acttttgtat agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt
    16681 ttcaacttct ccgctaggat agatatattc ataggggtct tgcgtagctg ttggcccaag
    16741 acgctccaaa ataccgttga ataactggcg ttgaatgcgc tggagtgctt gaccgctggg
    16801 tacgattggt tcgtccagct tttccatctc attttcttcg ctacgttgaa tttcagcgga
    16861 atcttctgta tcagtttcat tttcttgtaa ttcttggtta tctattcttt catcaaccac
    16921 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    16981 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    17041 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    17101 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    17161 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    17221 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    17281 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    17341 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    17401 atactccttt caaaaatatt tgtcttcttt cacaacaatc aac
//
LOCUS       DAZUKA010000055        17275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000055 DAZUKA010000000
VERSION     DAZUKA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.055"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACG8PN_001450"
     CDS             <1..420
                     /locus_tag="ACG8PN_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861604.1"
                     /translation="FLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTL
                     IIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPP
                     KIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            515..1399
                     /locus_tag="ACG8PN_001451"
     CDS             515..1399
                     /locus_tag="ACG8PN_001451"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4861605.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1623..4790
                     /locus_tag="ACG8PN_001452"
     CDS             1623..4790
                     /locus_tag="ACG8PN_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4861606.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4858..5355
                     /locus_tag="ACG8PN_001453"
     CDS             4858..5355
                     /locus_tag="ACG8PN_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS4861607.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5374..5838
                     /locus_tag="ACG8PN_001454"
                     /pseudo
     CDS             5374..5838
                     /locus_tag="ACG8PN_001454"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5835..6593
                     /locus_tag="ACG8PN_001455"
     CDS             5835..6593
                     /locus_tag="ACG8PN_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4861608.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6679..7419)
                     /locus_tag="ACG8PN_001456"
     CDS             complement(6679..7419)
                     /locus_tag="ACG8PN_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4861609.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7480..8733
                     /locus_tag="ACG8PN_001457"
     CDS             7480..8733
                     /locus_tag="ACG8PN_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4861610.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9055..9282
                     /locus_tag="ACG8PN_001458"
     CDS             9055..9282
                     /locus_tag="ACG8PN_001458"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4861611.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9405..9731
                     /locus_tag="ACG8PN_001459"
     CDS             9405..9731
                     /locus_tag="ACG8PN_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4861612.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9799..10512
                     /locus_tag="ACG8PN_001460"
     CDS             9799..10512
                     /locus_tag="ACG8PN_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4861613.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10822..11672
                     /gene="licT"
                     /locus_tag="ACG8PN_001461"
                     /pseudo
     CDS             10822..11672
                     /gene="licT"
                     /locus_tag="ACG8PN_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11793..13658
                     /locus_tag="ACG8PN_001462"
     CDS             11793..13658
                     /locus_tag="ACG8PN_001462"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4861614.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13672..15115
                     /locus_tag="ACG8PN_001463"
                     /pseudo
     CDS             13672..15115
                     /locus_tag="ACG8PN_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15223..15924
                     /locus_tag="ACG8PN_001464"
     CDS             15223..15924
                     /locus_tag="ACG8PN_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4861615.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16050..16562
                     /locus_tag="ACG8PN_001465"
                     /pseudo
     CDS             16050..16562
                     /locus_tag="ACG8PN_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(16960..17169)
                     /locus_tag="ACG8PN_001466"
     CDS             complement(16960..17169)
                     /locus_tag="ACG8PN_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4861616.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5762 a         2904 c         3713 g         4896 t
ORIGIN      
        1 tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac aaataaaaag
       61 gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc cgttttggtg
      121 ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg tgacatatta
      181 acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc tataaggtct
      241 gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg tttttatctg
      301 ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt tatttcctat
      361 atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt caaaaaataa
      421 atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat gctatcgtta
      481 tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat tagtggtaaa
      541 ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag caaaattgga
      601 atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt
      661 tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg gagatggaac
      721 agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt tcggattttt
      781 cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag aacctgagga
      841 agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa aaataaatga
      901 cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca atgatgtaga
      961 tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa tcaaacagtt
     1021 agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg aagaaattga
     1081 gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa cattacttcc
     1141 aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt cttctttgat
     1201 cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta cgaaaaatct
     1261 agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag aattgacgac
     1321 gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat tgccctcaca
     1381 tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga gaacaccgct
     1441 tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg tacaaaaaag
     1501 aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat atgaaagtac
     1561 aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga ggatggtgga
     1621 gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc cctctcttga
     1681 tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa tcagagaatc
     1741 aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact acagaatctc
     1801 aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag acctcagaag
     1861 tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt tcagcggatg
     1921 ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac gaatcagtaa
     1981 ctaagatccg actgattcag cctttgactt tagacaggga attagagatc aaacgagata
     2041 tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt tatatcaatg
     2101 agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat ccaggtggat
     2161 tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat ttttggggaa
     2221 gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg tatgatgggt
     2281 ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt gtttccggtg
     2341 tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa gcctattttt
     2401 gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg tatctaggga
     2461 aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca gcgccaggaa
     2521 tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat ggcgttagcg
     2581 atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc agtggttcgg
     2641 gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat cgcggaatca
     2701 tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca
     2761 atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc catgtatcaa
     2821 gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg aacaatggga
     2881 tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca gttatcgaca
     2941 tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg caaaagctga
     3001 cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga
     3061 acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt tttctagaaa
     3121 gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat atacgcgcag
     3181 attccggagc aactgtagat acaactggag atattgattt aaattttacg gcaggtgaaa
     3241 aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc atcagtgggg
     3301 atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac aaacggattg
     3361 gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat tcttctttaa
     3421 agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc gataactttg
     3481 aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg accgacttag
     3541 gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag acaggtgccg
     3601 gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt gaatccttaa
     3661 gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg
     3721 aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt attacttacg
     3781 cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa acagatgaaa
     3841 atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg ttcgaagttc
     3901 aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa
     3961 aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca gtcgatgtga
     4021 ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct cgcacgatca
     4081 caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct gttttgtcag
     4141 aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat caactttttg
     4201 caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact gctaagggtg
     4261 tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt gatacgccag
     4321 ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc
     4381 tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact ggtttgacac
     4441 aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat cagcgagaaa
     4501 cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat gatgcaggat
     4561 tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta gtgaatgaag
     4621 aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat tattcagaat
     4681 atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag atagctggta
     4741 attactcagc agagataaca tggacattag cagatgcgcc gacagaataa agatcaagga
     4801 ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc tttacttatg
     4861 atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga attcaatttt
     4921 gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata tataaatgta
     4981 aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg aacagataaa
     5041 tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg tgtgatcgaa
     5101 tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa aaacttagca
     5161 aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac actttcactg
     5221 aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa
     5281 aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa
     5341 gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt aaaaaaagct
     5401 tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa tccaaatgct
     5461 tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac aaaagagcct
     5521 tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt tgatttactt
     5581 gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt gaagggtgct
     5641 tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga tgcagcggac
     5701 caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga ctggcgatgg
     5761 ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat tttaaaagaa
     5821 agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct ggaagtctat
     5881 tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca gaaggaacga
     5941 tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg
     6001 atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga acatccggat
     6061 tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta ggtacagcaa
     6121 aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa acacaagtac
     6181 agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa ttatcagtca
     6241 aacgaacaga atttattggt acaacatctg atacgaataa actaactggt tcaaaggtga
     6301 aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg ccatctaccg
     6361 tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa
     6421 ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag aatgtggaac
     6481 aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta gacaactacg
     6541 tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg taaaatcata
     6601 tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct gctatgctta
     6661 cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga aacgttcctt
     6721 tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa attctaacac
     6781 ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag attttaaaag
     6841 taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag tattcactaa
     6901 tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga tactttcaat
     6961 atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa taaagatttt
     7021 atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt gacgaatttg
     7081 ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc gattgatgat
     7141 ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg catttttttt
     7201 acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga tatcgataaa
     7261 gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt gagagataga
     7321 agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga tccattcgct
     7381 gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc tattaaaaaa
     7441 atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac agtgacactt
     7501 tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc gaaacaactg
     7561 ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt acttcctatc
     7621 cctaaagtga gctttcctta cctagttggc ttattattga tgtttctttt ttattttact
     7681 acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt tttttgctat cactggactc
     7741 agcttttttc tggtttttga tctgatggat tatttaggaa agagacatct tggaacagca
     7801 agtttttttg cactacttat cgcatttatt ttctatctat tagtaaaaag tatagacaaa
     7861 cagacgagat ttctcaactt gctttttact tatccaactg aacactattt tggaggtatt
     7921 tgtattttat taagctgggg cgtgatttac ttggtattgg gaagtgttca cttccgctca
     7981 tctaattatc tgcttctatc tcttttgtcg atagtcatca gttttttttc tttgtttttt
     8041 tccattatgt tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg
     8101 tatcaaataa atgaagaata ttaccatcat ctagaggaat tcaaacatga ttataaaagt
     8161 ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt ggaattttta
     8221 gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca acagctaaca
     8281 aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa agcagaatcg
     8341 gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc gattgatctg
     8401 gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca tcgtgtagcc
     8461 gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg ttttagtata
     8521 gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc
     8581 tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa
     8641 aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt agaaatgtat
     8701 ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata agtttgagga
     8761 aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag aagcgtgatg
     8821 accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt gacacttccg
     8881 ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga agtgaaaagc
     8941 ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag ggaagagcaa
     9001 tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa aaaaatgctg
     9061 acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc ctataaccaa
     9121 gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag agagattcta
     9181 gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa
     9241 gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat gacatttatt
     9301 ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat gtaaaaaaat
     9361 ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa
     9421 aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg
     9481 ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg aaaacgccgg
     9541 aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat gtattgatct
     9601 attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt gaagaaaaat
     9661 tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca
     9721 cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg
     9781 agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc
     9841 ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca gatcaaggat
     9901 tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa agaagatctg
     9961 aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc aatgacgaca
    10021 gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc tagtatcgca
    10081 gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac gtcactactt
    10141 tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta tgccttagca
    10201 agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt agagatgggt
    10261 gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt ttttaaagaa
    10321 aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga aattgtcaca
    10381 ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg agaactggaa
    10441 agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa tctagagaca
    10501 aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc aaaggatatg
    10561 aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg
    10621 atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat atgaaaaggc
    10681 ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat tgttactgta
    10741 cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt ttttttattt
    10801 ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa tgtcgtcatt
    10861 tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa aaggactagc ttttcaaaaa
    10921 aagattgggg acgagatttc gccagaagcg gtccaaaaag aatttgttta agagattctt
    10981 ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg
    11041 tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt
    11101 atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac
    11161 tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga
    11221 aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg
    11281 gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga
    11341 tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg
    11401 tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc
    11461 agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc
    11521 aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa
    11581 cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag
    11641 tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc
    11701 tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat
    11761 tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg
    11821 tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt
    11881 tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg
    11941 acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag
    12001 cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag
    12061 aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt
    12121 ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga
    12181 tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg
    12241 tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc
    12301 agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg
    12361 gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata
    12421 cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg
    12481 taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag
    12541 tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt
    12601 taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg
    12661 gaagtttatg gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt
    12721 ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct
    12781 tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag
    12841 cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg
    12901 gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg
    12961 gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga
    13021 cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa
    13081 tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg
    13141 aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt
    13201 cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac
    13261 cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag
    13321 gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg
    13381 tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag
    13441 aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag
    13501 atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc
    13561 cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga
    13621 ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca
    13681 cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc
    13741 ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg
    13801 ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta
    13861 cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc
    13921 ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg
    13981 agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg
    14041 cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt
    14101 ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc
    14161 tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat
    14221 caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg
    14281 agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt
    14341 aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac
    14401 aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga
    14461 attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg
    14521 tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca
    14581 gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat
    14641 caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa
    14701 tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc
    14761 attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg
    14821 agccattgat catatggaag aggatggcac gattattgat gattatcgga tcgattattt
    14881 acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta
    14941 tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta
    15001 cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa
    15061 aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa
    15121 gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt
    15181 atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta
    15241 ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc
    15301 ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact
    15361 gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt
    15421 gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg
    15481 caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc
    15541 catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag
    15601 cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa
    15661 atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa
    15721 aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa
    15781 gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct
    15841 acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct
    15901 taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc
    15961 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat
    16021 taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac
    16081 gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc
    16141 cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc
    16201 gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg
    16261 gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg
    16321 ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg
    16381 gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg
    16441 gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag
    16501 aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt
    16561 aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga gacaggaatg
    16621 gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg
    16681 aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt
    16741 tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc
    16801 tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat
    16861 gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca
    16921 ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat
    16981 ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa
    17041 attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac
    17101 ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa
    17161 ataatacacg ttattggcat ataggacgaa atcccagtat cttctataag taagtcattt
    17221 catatcgatg aaaacacgtt atttattttt gttttatcta taaaatggct gaaaa
//
LOCUS       DAZUKA010000056        17153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000056 DAZUKA010000000
VERSION     DAZUKA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.056"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..1800
                     /locus_tag="ACG8PN_001467"
     CDS             361..1800
                     /locus_tag="ACG8PN_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4861617.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2095..2313
                     /locus_tag="ACG8PN_001468"
                     /pseudo
     CDS             2095..2313
                     /locus_tag="ACG8PN_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2562..2777
                     /locus_tag="ACG8PN_001469"
     CDS             2562..2777
                     /locus_tag="ACG8PN_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861618.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2994..3250)
                     /locus_tag="ACG8PN_001470"
                     /pseudo
     CDS             complement(2994..3250)
                     /locus_tag="ACG8PN_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3437..4348)
                     /locus_tag="ACG8PN_001471"
     CDS             complement(3437..4348)
                     /locus_tag="ACG8PN_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4861619.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4367..5371)
                     /locus_tag="ACG8PN_001472"
     CDS             complement(4367..5371)
                     /locus_tag="ACG8PN_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4861620.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5368..7491)
                     /locus_tag="ACG8PN_001473"
     CDS             complement(5368..7491)
                     /locus_tag="ACG8PN_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4861621.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7496..9943)
                     /locus_tag="ACG8PN_001474"
     CDS             complement(7496..9943)
                     /locus_tag="ACG8PN_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4861622.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9930..10319)
                     /locus_tag="ACG8PN_001475"
     CDS             complement(9930..10319)
                     /locus_tag="ACG8PN_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4861623.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10379..10882)
                     /locus_tag="ACG8PN_001476"
     CDS             complement(10379..10882)
                     /locus_tag="ACG8PN_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4861624.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10895..11119)
                     /locus_tag="ACG8PN_001477"
     CDS             complement(10895..11119)
                     /locus_tag="ACG8PN_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861625.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11223..13133)
                     /gene="ltrA"
                     /locus_tag="ACG8PN_001478"
     CDS             complement(11223..13133)
                     /gene="ltrA"
                     /locus_tag="ACG8PN_001478"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4861626.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13879..14016)
                     /locus_tag="ACG8PN_001479"
     CDS             complement(13879..14016)
                     /locus_tag="ACG8PN_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4861627.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14013..15197)
                     /gene="mobT"
                     /locus_tag="ACG8PN_001480"
     CDS             complement(14013..15197)
                     /gene="mobT"
                     /locus_tag="ACG8PN_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4861628.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15456..15746)
                     /locus_tag="ACG8PN_001481"
     CDS             complement(15456..15746)
                     /locus_tag="ACG8PN_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861629.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15736..16878)
                     /gene="dcm"
                     /locus_tag="ACG8PN_001482"
                     /pseudo
     CDS             complement(15736..16878)
                     /gene="dcm"
                     /locus_tag="ACG8PN_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16954..17118)
                     /locus_tag="ACG8PN_001483"
     CDS             complement(16954..17118)
                     /locus_tag="ACG8PN_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861630.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         4818 a         3681 c         2990 g         5664 t
ORIGIN      
        1 atatcatagc aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat
       61 atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca
      121 ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga
      181 attatcacta gttactatag agtaagttca aataaagcat ctccaactta catggttaca
      241 tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg
      301 ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt
      361 atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct
      421 atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct
      481 accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat
      541 ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa
      601 atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc
      661 aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg
      721 actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg
      781 gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc
      841 ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga
      901 atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat
      961 acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca
     1021 atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt
     1081 tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt
     1141 tctttcggta tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt
     1201 ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg
     1261 ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg
     1321 gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc
     1381 ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg
     1441 tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc
     1501 aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg
     1561 aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta
     1621 tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg
     1681 gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt
     1741 gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga
     1801 aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga
     1861 tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac
     1921 tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg
     1981 caagctaggt atcccaccga tggactacgt tttagaggct actggttatg aatacggagc
     2041 caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc
     2101 tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt
     2161 agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt
     2221 atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac
     2281 aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa
     2341 gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt
     2401 catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct
     2461 aattataata agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta
     2521 ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg
     2581 atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta
     2641 ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc
     2701 tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga
     2761 ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga
     2821 tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt
     2881 tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt
     2941 tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc
     3001 agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca
     3061 taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg
     3121 aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac
     3181 aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat
     3241 ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata
     3301 aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc
     3361 tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa
     3421 ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata
     3481 ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg
     3541 tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact
     3601 ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt
     3661 cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg
     3721 ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg
     3781 gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga
     3841 ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct
     3901 ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag
     3961 attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac
     4021 cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat
     4081 ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat
     4141 tcactacctt ctcttttata accacttttt catgagttgt atgttgatca atggcggtga
     4201 aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca
     4261 tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct
     4321 tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc
     4381 cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat
     4441 ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac
     4501 cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa
     4561 tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg
     4621 cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa
     4681 acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt
     4741 catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat
     4801 aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt
     4861 gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt
     4921 ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata atactatctt
     4981 gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt
     5041 tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca
     5101 taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat
     5161 actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt
     5221 cttctgagac actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt
     5281 caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta
     5341 atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg
     5401 atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt
     5461 cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc
     5521 ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg
     5581 tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt
     5641 cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat
     5701 tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg
     5761 gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag
     5821 attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc
     5881 tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg
     5941 ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg
     6001 atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt
     6061 tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg
     6121 tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat
     6181 gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc
     6241 gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa
     6301 tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac
     6361 caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc
     6421 taaactttca taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa
     6481 gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga
     6541 aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt
     6601 aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc
     6661 tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat
     6721 ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga
     6781 aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac
     6841 aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat
     6901 ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac
     6961 aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc
     7021 cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt
     7081 ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc
     7141 cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga
     7201 caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct
     7261 tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa
     7321 atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc
     7381 atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt
     7441 gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
     7501 ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg
     7561 gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt
     7621 ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca
     7681 aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat
     7741 tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat
     7801 tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa
     7861 gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa
     7921 agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg
     7981 tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat
     8041 ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg
     8101 caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc
     8161 cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg
     8221 tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta
     8281 aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat
     8341 aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat
     8401 ccgcaatttc tggcaaggtt tctttccagc caccccgttc cccttttgga tcgacaatca
     8461 cagcttgtcc accaaaaagg acggcgtaat aaactaagag attgttacta aaggactttc
     8521 cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt ggtaactgaa tccttgaccc
     8581 cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc cgtatctacg ttataaccaa
     8641 agtaaatgcc ttccaactcc cctaacatct gagtggcgcc aaaaccaagt ccggcgagaa
     8701 aatcagacgt aacatactgg atataatcat tcatgtatcg cttactagaa ggtaagaatt
     8761 cttcatgtag ccccatcata tcgccaaaag gccggactag ttttacattg gtatcatcat
     8821 aaaaatctag cacttcatca caccggcgct ttagttcatc tactgattcg gcacttactc
     8881 gaaccacata gctcagtttg tacatggatt ctttggactg atccaaggta gtttccaatt
     8941 catctacgga atccaatgca tctaagacat tggaatttgt ttcattgtca gattgatagg
     9001 catggttgtc caagtctttt aattcctttt tcttattgcg aacggtagca agtgcctttt
     9061 tgttcgtcac aatctccaca ttcatactag tgtcaattgg gaatgtaaat tgttgttgct
     9121 ggtaatagaa aagttctgag gagggaaact ccatttcccc cacaattgta ttaattgtta
     9181 agtacgctac atatgattca tgattctcat gttccatgca taagtaccgg ggcttttctt
     9241 caatcaaaca acgactggga cgtaataagt cgtaacgttt caccaaagtt tccgacttca
     9301 gctttttctt tggtagctga aattcatatt cttcaaatgg tgtacccttt tcgccataga
     9361 tatgttcaat caaatacatc agatcactcg gtgtgactcg tctgacttta aatcttcgag
     9421 ccaatttact ttccattagt ttttcaagct tggtatatcg gcgaatttct tcattggata
     9481 aggaaacaaa atcgcccatc aaatgatgat tcactccata gacaaattct tgaaagccgg
     9541 agaagaaaga tttcttgaga ttctttagat tgacctcttc atctgtcgca atcagtttga
     9601 agccaataaa aaagcgataa tcaatctgac tatcgccaat catggatact aatgcctctg
     9661 tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg cccggtaatt tccttttttg
     9721 atcgttcttg cgtggcacga atactacttt ctgtggcaat ttgtaaggca tgaatcttgc
     9781 cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg gacttgatat ttctgttcag
     9841 gtgacaaaaa ggagtaattg taaggtatta actcatagta ggcaaaacat tctccctctt
     9901 gattaaagac aagattattt tcaatatact taatcgggta catagttcac actccttacc
     9961 agagtaatgg gttcgtccca tcgtttcttc tgttctttga ctttctttcc tgcgaaagtg
    10021 accttaaatc gtaagtgata gctaataaaa gatcgtaaaa atcccaatgg tttcttaccg
    10081 tcaaacgttt tttgcgacat gaaccaagta actgccaccg gtatacctaa gtattttaaa
    10141 aaggccccct ctatcataga tagtggaggc acatgggcaa atacgatgac aaacagcaag
    10201 gataaaacaa accaagccat ctggttaaaa gtcacaggaa aaggcagttg aaagtcatta
    10261 attgcataaa tgaccttttc aactgcccaa atactggtgt agctttttat tttcttcatc
    10321 aaatcattcc tttaaaaaga aggatagttt agaaatcaac tacccttcaa taattttgtt
    10381 aatatggata ctcaaaaaca cctcgactcg ttaccaaata attgcctcca atctccaagt
    10441 ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg ttctggtact tctccaaaat
    10501 caccagcttc tacaatcaag aaatatgcta catcactcat atcatcgcaa tctggataat
    10561 agataatatc atccacatgc tctaccatct cctcaaaaga attgaagaaa gcatgttgaa
    10621 tctcagaagc aacttcacca actggcgttc cttcaagttc ctccgctaag ccacacaacc
    10681 gattaatttc ctcaattggc gtatactcat caatctcaaa gggaagttca taatcatgaa
    10741 ttgcatattc ctcgtattcg tcatttaacc caattcgttc tttcacttca tcaaaatcga
    10801 caggtggggt aaaccacgcc cccaccaatt caccttcgtt atatttcccg aggttcgcaa
    10861 tataaacacg catctgttcc atcgtgcttc ccccttacgc accgacaatt cggttaaaca
    10921 aactgagtaa gacatccttc accccagaag cattaaagac caatccgacc gcaatcaaag
    10981 caatcaccaa aaagccaatc agtttggaaa actcccgctt aaatcctaaa aacaaaccaa
    11041 tgaccacaat tgccagtaag actaaggatt gggcattggt taaaaaccat tgatatatgg
    11101 ataggtaagt tcctgatatt ataaatatct caaacttttc cccccattca cacggagcgt
    11161 gcgactttca ccgcactccg cgttccatcg tgtttatatt gaaaagtatt tatgtagtag
    11221 acctatatca agtttaattc ttgattgcca gctaatactc tatatttatt tagtcttatg
    11281 agctggactt tatttagctt caatagagct atatattttt gtattgtttc ttctgtttta
    11341 gcgtgtatta gacggtgtac cgcctctttt accaaaataa gatttttata atcgtcattt
    11401 ccaccagctc cagttggtac aatatgatga caatgtattt cattagcttg ttgaaattct
    11461 tccagagtta tagcacattt tccatgttga gcagaaaata gcgaaattcg attgtctgcg
    11521 tattctattg tgcgattttc caaagataat ttcattaact ggtacatcag taatttatca
    11581 acacttagat tggtgtggat aaattttctt ccctctagag tgtagcaatt cgctaaatac
    11641 acctttgcca ttggttttct gtgttggata tatccaacag ggaaaatagg ttcgccctcg
    11701 ccagcaacat accgaagcat tttagattta ccatatcttg ttgcctcctt tgtcgtaagt
    11761 tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac ctaatctgtt agtgaagata
    11821 gccattacag ctctattcag tttcatacaa tccagattta cattcgttgc aatctgataa
    11881 taattttgca tacctaatac cattgaatta taaagtcttg tttcttctaa ttccgttttc
    11941 ccttttgagg gagaagcaat tttctttgcc tgtgatacca gcttgtcttt ttggtttttg
    12001 aattgtttat cacttatatg tgatttaaca actaacttct ttcttctcgg tatcatttta
    12061 atcttgaaac caagaaagtc ggagtatctt tttctaacat ttacaattct tgttttttct
    12121 tgtgatactt ccaattttaa tcgttgttca atccattgag tgattgctat tttggctttt
    12181 tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat ctgcataacg aaccatatac
    12241 atttccttta actttgtttt cttcatttct ttgtaagcgt tgcttttaca tgggctaccg
    12301 cttttattga tatgaaccac atagtttttt gtaacaggat tttcctgcca ttggctttct
    12361 acccaatggt ctaactcatt aagaacaatg tttgctagta gtggggatat tattccccct
    12421 tgcggagttc ctttctctgg atatgttatt gtaccgttag tcattttgat tggagctttt
    12481 aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt
    12541 tttatcagct tggaatggtt cacgttgtca aagaaaccct taatatcaaa ttcaatcaca
    12601 taatgtagct ggctgatttg taggcggtta taggttgctt ttattgcatt ttcaactgac
    12661 ctattaggtc taaaaccata gctgttttca ctaaatttag cttcgcagat tggttctaat
    12721 acttgtttta tacattgttg tataagcctg tcccacatac aaggaatacc tagaggtctg
    12781 gttttaccat ttggttttgg tatttctttt cgcctcactg gcttaggtcg atacccatgt
    12841 ggactgcctg caacaatgta tcttactttt ttgacaactg tttctgctgg caatttgcca
    12901 atatctttaa ttattgtacc gtctgttcct gctgttttac tgcctgcatt tgctttgata
    12961 tttcgatatg ccagtaatat attttctctc gataggataa tatccataag atgagtgaaa
    13021 atatctccat tcttactttt ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat
    13081 tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt tatttttagg cataaggctt
    13141 cactccttta cttgaaattg agtgagcttt tttatcttac tcgtaatcct tattgaaaca
    13201 acataatact tttctaatat atacacgact tgagggcatt cctccattct cattacaaga
    13261 atttcttagg ttcgccctct gcttttcagc attagttcaa ctgcttattg ttcttcgtca
    13321 gtttattctc ttgatgagaa cccaatacct ttcctcgttc cgataatctt atctatacat
    13381 atatatcctt aggtcactag ctataagcct gctggcttga taccgcctgt aacgttatat
    13441 ggcatttcat aacctatatt cttactttgc cacaccagct acgatttctc gtacctttgc
    13501 atttctacaa agttcaaatt tagacccgta cattcgctgt ttcgtcagtt ccttatagaa
    13561 cattctaacc atagatattt tatagacccc cgacctgttg gatacgctac ccacctctgg
    13621 atagttttcg atagaagata tatgttcatc aactattacg gtatctttca gccgtcttta
    13681 ccgagcttca aacattctga gtttcctcat acaatgcttg tcggaggctc agggcaggcg
    13741 tttcagagcg ttacttcatc attccacctg tcagattatc agttctccta ataaccaaac
    13801 tttttcgttc ttttattagt gccttagctt ttcactaagg aacgagtcgc acataaattt
    13861 tgtccgaaat tcatcttgtc actttccttt cttttccatt gctcgatccg ctgctgcact
    13921 tgcattcatc aagcacatca cagtaacacc aagcatactg ccaccaaata ccaaaaaaat
    13981 atcttttaca attcccatga cgattcctct tttcatataa tcaaattttc aattgccata
    14041 ctttgttgct ccaaaatctt tttatgtcgg tctgataagc gtgcattctg taccatatcc
    14101 tggatatagg ttgtccgatt caatcgatcc aactccttag ccattttcca cgtaggggcg
    14161 acttgtcggc ctagccaatt caaggttcgt tcaatggtat acggttttgg ctcggaagtt
    14221 aattgaatct ctttccgatt ccgtccaata aaacgccccc attgttcatt caagggccag
    14281 ttggttcgcc ttttaccttc tactttatcc gcaaatcgca agtaacgatt gataatggaa
    14341 aacgtggtgc tctcaatgct acgacctttt aacaaatctt gaatcgcatg aaaagcacgg
    14401 tcatttttca gtcgaatctc aaaacgattt ttcgtctcag tttggtctaa tggaatccct
    14461 aacttgatat attgttcata gtctttttca tacaaacaga aataaacctc actcttgaga
    14521 ctgccgatat ataaggtatt gcccatgccg tccttttcat cagcctttaa aagttccccg
    14581 gaacgataac ttttaaaggt acgaaacaag gagatacatt cttccttctg acattttttc
    14641 gttagctcag gtatatccaa tagccccact cgatcattga ttgccaagtc caagcgtttc
    14701 atcacgccac cggcctttag acaatcctcg aaaaagtcgt accagctacg cttttgagcg
    14761 agtaaaaaag tttcaaattg tcggcaacct cgccccttta attctaaaag cacaccttta
    14821 tcttcttcat gagacaacat caccacaata tcacccatga catactgttc ttgataggag
    14881 taaaaggcat aatcttcatg gagcatatac tcaaatttca aatggagaat ttcttgaata
    14941 atttttcgtg catccgttgt gggaaagcga atccttacat agtcaaacag taacgtcaaa
    15001 ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt tcctcatggt ttttatagat
    15061 gcggttacgc caccattttc tatctgattt aagtacggcc gactaagtcc acaagccgta
    15121 gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt cttttaatag ccgataatct
    15181 aaattccgtt gtgccaagtt ctcacctcca aattcaaggt gttaactttt tcaaatgagt
    15241 taacacctat acaaattact tactatcttt gatacaacaa gcattcttaa tggttgctta
    15301 cttatttttt gtgtttttgt acccccctgt tagatacggg ggtgagacat catggtcggc
    15361 taacgccgtc catgaacagg cacagcacca cggaggattt ctgcttcgcg aatcctccgc
    15421 ggtcagcgcc taacagtcag ttgtgttggt ttaggttact catctgtaat accatttact
    15481 tccaactctg ctttcatttc taacaagtat tgacgaatat ctggttcttt tgcttggtcc
    15541 gcaatccgct caaattctgc aattacttga tttatttcaa ttcgagcatc cgtctcctca
    15601 ttcattaaat atggatcgcc agcgactttg acaccattct tgcgaatatc aaattttaac
    15661 cgtcgcaatt gttcttctaa ttctgggtct gactgaatct ttccaatcac ccaataaatt
    15721 tctccaatca actcatcata aaccatgtac atccaactct tttaatcttc tggcaatatc
    15781 aaaaatgacc ggaactgtta cagagtttcc ggcttgctta tacaactgac tatctgaatt
    15841 gacttgagaa tctcgatcaa aagcccaatc gggaaagccc tgaagtcgcc aacactctct
    15901 aggtgttagc ttccgaattt gatactgatc cgtgacgatc gcttgttggt ctcctgttag
    15961 taaggtatga acactctgtt ttcccagacg acctcggcga cttgtggaat taggaaaagc
    16021 cagattcaca ctgtcttctg gcttcacctc ttgatattct gtttcatttt ttcgaatcat
    16081 gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg gttccagtaa cttcataaac
    16141 tcgattacct tgttcaaact ttcccggtaa tagtcctgct acagagattc catcccttgc
    16201 tggcagtgcg attttgattc cttcatcacc tgttaaggtt ggcgctacgc catctgaacc
    16261 atagacttgt ccacccattc ctcgattgga aggattgata tttaccaaat ttctgatgtt
    16321 ttgatcagat gttgaagacg gtccaatgat aggaaatact tgttcggtac atgcgtctcg
    16381 agaatgtccg ataaggaata tccgctcccg gctttgggga atgtaggcct ttgagttaag
    16441 cacctaccac tccacgtcat accccaatcc atccagcgtt cggaggatcg tctcgaacgt
    16501 agcccctcct tcgtgattga gcaatcctcg gacgttctca aggaataaaa gcttaggtcg
    16561 gagaagagtg gtgaaccgag cgatttcaaa gaataaagta cctcgagtat cggaaaatct
    16621 ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat ccgccgcaaa ggatgtccac
    16681 tggtccgagg gattgaataa actcatctga tacacttgtg atgtcatgca tttccacctc
    16741 cttacttgta tcatgaattg ctttataact acgtctagca aatttatcaa tttcacaaaa
    16801 accgatacaa tgatggctcg cctgttccat acctaaacga aagccaccaa taccggcgaa
    16861 taaatctaaa aatctcatac attacctcat tcattgcaaa gaaaaaagcc aagaacattt
    16921 tgctcttggc tcatacatta ttaatttgtc ctactatttt ttatttaatc caactaattt
    16981 ccgttgtttt tcattggcct gatcggctac atatttaatc accgttttga actcttttgc
    17041 agtagcattt tccgataaat ccgcaatgct cttattgtat ttctttacaa ttttcgttac
    17101 ttgttctttc tcagtcatgt aattgaacct ccaaatcttt cctactatac cct
//
LOCUS       DAZUKA010000057        16893 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000057 DAZUKA010000000
VERSION     DAZUKA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.057"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..574)
                     /locus_tag="ACG8PN_001484"
     CDS             complement(62..574)
                     /locus_tag="ACG8PN_001484"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4861631.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(588..2885)
                     /gene="recJ"
                     /locus_tag="ACG8PN_001485"
     CDS             complement(588..2885)
                     /gene="recJ"
                     /locus_tag="ACG8PN_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4861632.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3027..3470)
                     /locus_tag="ACG8PN_001486"
     CDS             complement(3027..3470)
                     /locus_tag="ACG8PN_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4861633.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3471..4259)
                     /locus_tag="ACG8PN_001487"
     CDS             complement(3471..4259)
                     /locus_tag="ACG8PN_001487"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4861634.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4256..5197)
                     /gene="rnz"
                     /locus_tag="ACG8PN_001488"
     CDS             complement(4256..5197)
                     /gene="rnz"
                     /locus_tag="ACG8PN_001488"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4861635.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5374..5961)
                     /locus_tag="ACG8PN_001489"
     CDS             complement(5374..5961)
                     /locus_tag="ACG8PN_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4861636.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6182..7495)
                     /gene="obgE"
                     /locus_tag="ACG8PN_001490"
     CDS             complement(6182..7495)
                     /gene="obgE"
                     /locus_tag="ACG8PN_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4861637.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7783..8793
                     /gene="gap"
                     /locus_tag="ACG8PN_001491"
     CDS             7783..8793
                     /gene="gap"
                     /locus_tag="ACG8PN_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4861638.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8878..9813)
                     /locus_tag="ACG8PN_001492"
     CDS             complement(8878..9813)
                     /locus_tag="ACG8PN_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4861639.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            9999..10958
                     /locus_tag="ACG8PN_001493"
     CDS             9999..10958
                     /locus_tag="ACG8PN_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4861640.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11044..12138)
                     /locus_tag="ACG8PN_001494"
     CDS             complement(11044..12138)
                     /locus_tag="ACG8PN_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4861641.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12327..13088
                     /locus_tag="ACG8PN_001495"
     CDS             12327..13088
                     /locus_tag="ACG8PN_001495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4861642.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13203..13643)
                     /gene="fabZ"
                     /locus_tag="ACG8PN_001496"
     CDS             complement(13203..13643)
                     /gene="fabZ"
                     /locus_tag="ACG8PN_001496"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4861643.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13863..14618
                     /gene="fabI"
                     /locus_tag="ACG8PN_001497"
     CDS             13863..14618
                     /gene="fabI"
                     /locus_tag="ACG8PN_001497"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4861644.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14728..15780)
                     /locus_tag="ACG8PN_001498"
     CDS             complement(14728..15780)
                     /locus_tag="ACG8PN_001498"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4861645.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15906..16826
                     /locus_tag="ACG8PN_001499"
     CDS             15906..16826
                     /locus_tag="ACG8PN_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4861646.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4915 a         3640 c         2962 g         5376 t
ORIGIN      
        1 tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg
       61 cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat
      121 ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt
      181 cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt
      241 gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat
      301 aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga
      361 ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta
      421 ccatatcaat tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag
      481 cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt
      541 cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac
      601 catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg
      661 agccgtcgct ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt
      721 tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa
      781 attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata
      841 tcaattcgtt cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct
      901 accccattca aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa
      961 taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga
     1021 gagtttggtt ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc
     1081 gcagcttcct ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa
     1141 aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact
     1201 tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat
     1261 tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga
     1321 gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc
     1381 agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa
     1441 aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc
     1501 tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc
     1561 aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt
     1621 tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct
     1681 cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta
     1741 gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt
     1801 ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata
     1861 tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt
     1921 ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac
     1981 gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg
     2041 ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct
     2101 tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa
     2161 acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact
     2221 aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact
     2281 ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc
     2341 gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca
     2401 ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg
     2461 atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca
     2521 tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct
     2581 ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt
     2641 tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga
     2701 tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca
     2761 tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc
     2821 atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt
     2881 ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga
     2941 aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt
     3001 gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat
     3061 tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac
     3121 tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct
     3181 tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc
     3241 agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc
     3301 agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg
     3361 aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa
     3421 actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac ctactttttg
     3481 ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat
     3541 gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg
     3601 atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt
     3661 ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta
     3721 acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca
     3781 aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg
     3841 atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa
     3901 accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta
     3961 aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact
     4021 tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg
     4081 aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat
     4141 gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt
     4201 tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg
     4261 cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt
     4321 tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg
     4381 cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt
     4441 gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc
     4501 tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg
     4561 tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat
     4621 tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg
     4681 gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat
     4741 gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag
     4801 aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat
     4861 aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg
     4921 gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg
     4981 gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg
     5041 gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa
     5101 cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag
     5161 gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt
     5221 tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca
     5281 aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca
     5341 gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt
     5401 gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg
     5461 attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt
     5521 aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc
     5581 agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac
     5641 ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa
     5701 ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc
     5761 acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg
     5821 tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc
     5881 atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa
     5941 aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta
     6001 cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg
     6061 acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt
     6121 ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg
     6181 cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac
     6241 cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca
     6301 ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata
     6361 agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc
     6421 catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt
     6481 ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa
     6541 ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt
     6601 tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt
     6661 gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt
     6721 cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg
     6781 ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa
     6841 tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta
     6901 gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa
     6961 cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata
     7021 ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg
     7081 gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg
     7141 ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat
     7201 ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca
     7261 tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat
     7321 ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt
     7381 ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt
     7441 ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta
     7501 agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc
     7561 ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac
     7621 cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat
     7681 tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgagg
     7741 atgccgtttc acagcacccc taataaagga ggaacttttg atatgacagt aaaagtaggt
     7801 attaacggat ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga agtctcagat
     7861 gatattgaag tagtagcaat caacgactta acaagtccaa ctatgttagc acaattattg
     7921 caatttgact ctactcatgg tacttatcct ggcacagtta ctgcaacaga agactcaatc
     7981 gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg
     8041 gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg gtttttatac atctgaagaa
     8101 aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag ttatttctgc cccagctggt
     8161 gctatgaaaa ccatcgtcta taacgtcaat gatgatacat tagatgaaaa cgacaaaatc
     8221 atctcagcag gttcatgtac aacaaactgt ttagcaccta tggcttattt cttaaataat
     8281 gaatttggga tcgaagttgg tacaatgaca acagttcatg cctacacttc cacacaaatg
     8341 ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat
     8401 actatcccac attctactgg tgctgcgaaa gcaatcggct tagttatccc tgagctacaa
     8461 ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg atggttcatt aactgaattg
     8521 gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat caaaaaacac
     8581 acagttgaca atccttcatt cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt
     8641 ggaacaactc aaggttcgat ctttgaccca acgcaaacag aagtaacaac tgctggtgat
     8701 ttccaacttg ttaaaactgt tgcatggtat gacaatgagt atggatttac ttgccaaatg
     8761 attcgcttac tagaaaaatt cgctaacttg taatttgata gatcaatcga taaaagaagc
     8821 ctttgactgg atccagtcaa aggcttcttt cttttcatcc cctactttta aactcgttca
     8881 atgaacatat tggcttaaaa attgttggac tacttgctga tatttttcag gatctgtctt
     8941 gtatgcttcc gcatgctcgg ctccttttac aacatacttt tcttttggtc ccttcgttgc
     9001 acgatagacc ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc catgtataaa
     9061 gagcattggg cgctggtttt tcttcaattg ttccactgca tttgcttcac cgaaaaagta
     9121 gccagcacgg acttttgtca ctagactcgt tacttgcata agcggaaatg ctggaagact
     9181 aaacatatcg tctagctgat aagcaagctc atcatgaacg gaagaataac cgcaatcttc
     9241 aatgattgct ttcacattat ctggtagttt ttctccgcta gtcatcataa ctgttgctgc
     9301 tcccatgctg acaccgtata gaacgatttc ttgcgatttt ccattgtttt ctagcacttt
     9361 gtttatccac tgtacatagt ctttacgctc tggccatcca aatccaatgt aatccccttc
     9421 gctttttcca tgtcctcttg catcaggaac aaggacattg taacccatat catgaaacat
     9481 ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag ccatgagcca caagagctgt
     9541 tttttcactt ttcgtttcag caggaagata aatcgctgat aatttcagac cgtcttttga
     9601 agtgatggtc caatcgcttc ggttttcttt tgaactaaac catttatgtg catccgtttc
     9661 tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct cagaaggcac
     9721 aacagcataa ttataaaaat aattccctgc aaacactaac gcaacaatca gagctaaagc
     9781 gacgcctaaa aggattttca tccaaagttt cataaatcat ccatctcatt tctctttttt
     9841 catataacct tgaattagta taatcaaaga atctctacta tgcaatgttc atttttataa
     9901 cccgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac actcaaattg
     9961 tatttccgac gaaagaaaac aaaggagtgt aaccataaat gacctacaaa catcttacca
    10021 tagacgaact gacaatgata gaatcatatt atcttcaaca taataagccg gttgaaatcg
    10081 ctaaccgaat gggacgtgct atacaaacta tttataatgt agtcaataag ttcaagcaag
    10141 gcaagactgc tcttgattat tggcaccagt ataaagaaaa taagaaaaaa tgtggtagaa
    10201 aagtcattca attacctgct catgaagtag attacattaa agagaaagtc actcttggtt
    10261 ggacgcctga cgtcattatc gggcgaaaag aaaggtctgt ttcatgcggt atgagaacac
    10321 tttatcgttt attttctaaa ggaatatttg atattgacac actaccgatg aaaggtaaaa
    10381 gaaaacccaa tggccatcag gaaaaacggg gaaaacaaca gtatcagcgc tcaatccatg
    10441 atcgacctga taattatcct gatttcaatt ctgagtttgg tcaccttgaa ggtgatacga
    10501 tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta tctaaagtca
    10561 ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc cttaatcaat
    10621 ggttttctcg cttccctaaa aacttcttta aatctattac gtttgactgt ggaaaagaat
    10681 tttctaactg gaaagccatt agtaaccaac atgatattga tatatatttt gcggaccctg
    10741 gaacaccttc tcaacgccca ttaaacgaga attctaacgg gattctgcgt cgtaatggac
    10801 tgccgaaatc aatggatttt agaaaagtga atcagacatt tatttccagt gtcagcaatc
    10861 aacgtaatca tattccaaga aaatcattga attacagaac accaatcgaa atatttttga
    10921 gctatgtaca agaagcattt tattctagct taatttgaca aatcataacc tataaaaaaa
    10981 caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc aataactaat
    11041 tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa agtcaaggag
    11101 gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt ttttcatatc
    11161 cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt caatcagtac
    11221 atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt tgtagtatac
    11281 acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac tatgaaggtt
    11341 atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta ctttcaatgt
    11401 tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac ttgcgtacgc
    11461 atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca cttgtgtcgc
    11521 aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag gccaagggcc
    11581 aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt ttctgaattt
    11641 ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag gataaaaatt
    11701 atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt caacgtaatt
    11761 cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg tcatttttgc
    11821 ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca tcttatggaa
    11881 aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat tgatatcaac
    11941 cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt ctgaaagaac
    12001 tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat acccatactt
    12061 atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg gataaatcga
    12121 gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt gtatccactt
    12181 acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt aaaataaatg
    12241 ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt aaagataaca
    12301 aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta agtattagtt
    12361 tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact tcttctagtt
    12421 cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc agtagcgtcg
    12481 atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat gaagatctgg
    12541 tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa cacgcaccaa
    12601 aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga ctgactttcc
    12661 atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat ggcactggag
    12721 gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt tataatataa
    12781 gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc cagttcttta
    12841 tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat cctcaagcaa
    12901 tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat actgtcttcg
    12961 gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa acagatagtt
    13021 cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa gaagcaaaac
    13081 aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta ggattcaggc
    13141 gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat caggttttat
    13201 agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt tttatcatct
    13261 acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat ttcaaaatga
    13321 catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt gatcccaccg
    13381 atataagctc tttttcctag aaactcttct gattttagga taagtatcga acctgcttgt
    13441 gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa gtgaccttgg
    13501 aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc tggttctaaa
    13561 gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc cattacttca
    13621 gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg ccattttcat
    13681 tactttgact atcaaagtat catattccta aatatttagc aatctttttt taatttaaga
    13741 aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag aagttatttt
    13801 agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa aggagagtca
    13861 ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac aaaaaaagca
    13921 ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc tatacttacc
    13981 aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca ttcactgtag
    14041 aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc ggtgaatatg
    14101 ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa gaattgtccg
    14161 gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc agcagctact
    14221 ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc gggatcgtta
    14281 gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg ggtattgcga
    14341 aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct aaaggaattc
    14401 gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt gttaaagact
    14461 atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt gtgacgatcg
    14521 aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga atcatcggtg
    14581 atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca ttctttaaag
    14641 taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt tttcttttta
    14701 cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc cttctggtgc
    14761 ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg ttactggtcc
    14821 tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc caccgattgg
    14881 agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta gatcatcaat
    14941 gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact gcaacaagga
    15001 atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt ttccgtcttt
    15061 tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg tggctggatc
    15121 aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa gtaaactcgc
    15181 tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac gatcgtaatc
    15241 agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta ctggcgtaca
    15301 gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac cgaccccaat
    15361 cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa aatcttcatt
    15421 tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca ttggcatcag
    15481 caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa agagattgac
    15541 cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac cataatgaag
    15601 cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc cttcttccca
    15661 ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag aaaaacctag
    15721 attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac attttatcat
    15781 tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt tatttttgct
    15841 atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt agaaaagagg
    15901 attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc gagacattga
    15961 cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg attgattggg
    16021 acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg atcgtccttt
    16081 tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat cagtattcta
    16141 aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta aatactattt
    16201 ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc ggtgtgccga
    16261 tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt ggtgcccaag
    16321 ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag atggatgttg
    16381 gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct gttggtatcc
    16441 gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat cgaaacatca
    16501 ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt cggattgttc
    16561 ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt acattagctg
    16621 aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg gatcttggaa
    16681 acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa tatgccttaa
    16741 aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc acgattccga
    16801 acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc tcactaaaaa
    16861 gagcgtggga caaaagtaaa gaacactttt gtc
//
LOCUS       DAZUKA010000058        16850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000058 DAZUKA010000000
VERSION     DAZUKA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.058"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(361..3066)
                     /locus_tag="ACG8PN_001500"
     CDS             complement(361..3066)
                     /locus_tag="ACG8PN_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4861647.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3372..4319)
                     /locus_tag="ACG8PN_001501"
     CDS             complement(3372..4319)
                     /locus_tag="ACG8PN_001501"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4861648.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4509..4778
                     /gene="rpsN"
                     /locus_tag="ACG8PN_001502"
     CDS             4509..4778
                     /gene="rpsN"
                     /locus_tag="ACG8PN_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4861649.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4954..5316)
                     /locus_tag="ACG8PN_001503"
     CDS             complement(4954..5316)
                     /locus_tag="ACG8PN_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4861650.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5327..6448)
                     /gene="alr"
                     /locus_tag="ACG8PN_001504"
     CDS             complement(5327..6448)
                     /gene="alr"
                     /locus_tag="ACG8PN_001504"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4861651.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6464..6814)
                     /gene="acpS"
                     /locus_tag="ACG8PN_001505"
     CDS             complement(6464..6814)
                     /gene="acpS"
                     /locus_tag="ACG8PN_001505"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4861652.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6944..8455)
                     /locus_tag="ACG8PN_001506"
     CDS             complement(6944..8455)
                     /locus_tag="ACG8PN_001506"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4861653.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8777..10129)
                     /gene="murF"
                     /locus_tag="ACG8PN_001507"
     CDS             complement(8777..10129)
                     /gene="murF"
                     /locus_tag="ACG8PN_001507"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4861654.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10282..11358)
                     /locus_tag="ACG8PN_001508"
     CDS             complement(10282..11358)
                     /locus_tag="ACG8PN_001508"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4861655.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11554..13299
                     /locus_tag="ACG8PN_001509"
     CDS             11554..13299
                     /locus_tag="ACG8PN_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4861656.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13579..14016)
                     /locus_tag="ACG8PN_001510"
     CDS             complement(13579..14016)
                     /locus_tag="ACG8PN_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4861657.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14000..15373)
                     /locus_tag="ACG8PN_001511"
     CDS             complement(14000..15373)
                     /locus_tag="ACG8PN_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861658.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15695..16183)
                     /locus_tag="ACG8PN_001512"
     CDS             complement(15695..16183)
                     /locus_tag="ACG8PN_001512"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4861659.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16205..16807)
                     /locus_tag="ACG8PN_001513"
     CDS             complement(16205..16807)
                     /locus_tag="ACG8PN_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4861660.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4733 a         3609 c         3063 g         5445 t
ORIGIN      
        1 aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt
       61 tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg
      121 gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca taagaagctg
      181 tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac
      241 acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac
      301 tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt
      361 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
      421 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
      481 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
      541 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
      601 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
      661 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
      721 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
      781 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
      841 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
      901 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
      961 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
     1021 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
     1081 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
     1141 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
     1201 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
     1261 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
     1321 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
     1381 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
     1441 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
     1501 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
     1561 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
     1621 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
     1681 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
     1741 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
     1801 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
     1861 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
     1921 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
     1981 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
     2041 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
     2101 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
     2161 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
     2221 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
     2281 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
     2341 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
     2401 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
     2461 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
     2521 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
     2581 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
     2641 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
     2701 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
     2761 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
     2821 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
     2881 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
     2941 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
     3001 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
     3061 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
     3121 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
     3181 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
     3241 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
     3301 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
     3361 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
     3421 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
     3481 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
     3541 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
     3601 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
     3661 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
     3721 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
     3781 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
     3841 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
     3901 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
     3961 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
     4021 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
     4081 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
     4141 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
     4201 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
     4261 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
     4321 gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg
     4381 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
     4441 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
     4501 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
     4561 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
     4621 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
     4681 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
     4741 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
     4801 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
     4861 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
     4921 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
     4981 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
     5041 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
     5101 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
     5161 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
     5221 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
     5281 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
     5341 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
     5401 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
     5461 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
     5521 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
     5581 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
     5641 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
     5701 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
     5761 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
     5821 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
     5881 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
     5941 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
     6001 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
     6061 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
     6121 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
     6181 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
     6241 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
     6301 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
     6361 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
     6421 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
     6481 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
     6541 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
     6601 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
     6661 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
     6721 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
     6781 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
     6841 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
     6901 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
     6961 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
     7021 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
     7081 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
     7141 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
     7201 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
     7261 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
     7321 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
     7381 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
     7441 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
     7501 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
     7561 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
     7621 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
     7681 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
     7741 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
     7801 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
     7861 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
     7921 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
     7981 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca
     8041 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
     8101 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
     8161 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
     8221 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
     8281 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
     8341 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
     8401 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
     8461 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
     8521 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
     8581 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
     8641 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
     8701 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
     8761 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
     8821 tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat
     8881 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
     8941 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
     9001 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
     9061 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
     9121 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
     9181 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
     9241 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
     9301 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
     9361 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
     9421 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
     9481 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
     9541 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
     9601 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
     9661 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
     9721 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
     9781 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
     9841 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
     9901 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
     9961 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
    10021 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
    10081 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
    10141 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
    10201 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
    10261 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
    10321 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
    10381 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
    10441 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
    10501 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
    10561 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
    10621 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
    10681 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
    10741 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
    10801 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
    10861 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
    10921 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
    10981 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
    11041 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
    11101 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
    11161 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac
    11221 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
    11281 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
    11341 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
    11401 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
    11461 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
    11521 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
    11581 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
    11641 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
    11701 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
    11761 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
    11821 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    11881 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    11941 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    12001 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    12061 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    12121 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    12181 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    12241 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    12301 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    12361 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    12421 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    12481 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    12541 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    12601 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    12661 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    12721 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    12781 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    12841 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    12901 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    12961 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    13021 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    13081 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    13141 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    13201 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    13261 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    13321 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    13381 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    13441 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    13501 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    13561 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    13621 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    13681 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    13741 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    13801 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    13861 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    13921 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    13981 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    14041 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    14101 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    14161 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    14221 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    14281 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    14341 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    14401 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    14461 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    14521 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    14581 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    14641 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    14701 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    14761 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    14821 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    14881 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    14941 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    15001 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    15061 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    15121 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    15181 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    15241 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    15301 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    15361 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    15421 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    15481 aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc
    15541 cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc
    15601 aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc
    15661 gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat
    15721 aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc
    15781 cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct
    15841 ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc
    15901 cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt ccatttatct
    15961 cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac
    16021 cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca
    16081 cttgtttttg accaatgatc ggcacaaaaa cttccggcca tacttcttgt atgactgcca
    16141 tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt
    16201 tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac
    16261 ccaccgacca cggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg
    16321 tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa
    16381 ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact
    16441 tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc
    16501 cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa
    16561 acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga
    16621 tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt
    16681 gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga
    16741 aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt
    16801 tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAZUKA010000059        16714 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000059 DAZUKA010000000
VERSION     DAZUKA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.059"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACG8PN_001514"
     CDS             complement(251..1462)
                     /locus_tag="ACG8PN_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861661.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACG8PN_001515"
     CDS             complement(1581..2216)
                     /locus_tag="ACG8PN_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4861662.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACG8PN_001516"
     CDS             complement(2352..3005)
                     /locus_tag="ACG8PN_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861663.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACG8PN_001517"
     CDS             complement(3108..3314)
                     /locus_tag="ACG8PN_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFS4861664.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACG8PN_001518"
     CDS             complement(3390..4136)
                     /locus_tag="ACG8PN_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS4861665.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACG8PN_001519"
     CDS             4296..4520
                     /locus_tag="ACG8PN_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFS4861666.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACG8PN_001520"
     CDS             4544..5305
                     /locus_tag="ACG8PN_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFS4861667.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACG8PN_001521"
     CDS             5317..5574
                     /locus_tag="ACG8PN_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861668.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACG8PN_001522"
     CDS             5666..5917
                     /locus_tag="ACG8PN_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFS4861669.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5917..6066
                     /locus_tag="ACG8PN_001523"
     CDS             5917..6066
                     /locus_tag="ACG8PN_001523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861670.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACG8PN_001524"
     CDS             complement(6180..6587)
                     /locus_tag="ACG8PN_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861671.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACG8PN_001525"
     CDS             6748..6972
                     /locus_tag="ACG8PN_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861672.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACG8PN_001526"
     CDS             6982..7176
                     /locus_tag="ACG8PN_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861673.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACG8PN_001527"
     CDS             7179..8201
                     /locus_tag="ACG8PN_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS4861674.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACG8PN_001528"
     CDS             8164..8979
                     /locus_tag="ACG8PN_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFS4861675.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACG8PN_001529"
     CDS             8997..9779
                     /locus_tag="ACG8PN_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861676.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACG8PN_001530"
     CDS             9779..10627
                     /locus_tag="ACG8PN_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4861677.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACG8PN_001531"
     CDS             10624..10866
                     /locus_tag="ACG8PN_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861678.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACG8PN_001532"
     CDS             11009..11377
                     /locus_tag="ACG8PN_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4861679.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACG8PN_001533"
     CDS             11392..11553
                     /locus_tag="ACG8PN_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4861680.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACG8PN_001534"
     CDS             11550..11876
                     /locus_tag="ACG8PN_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861681.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACG8PN_001535"
     CDS             11873..12688
                     /locus_tag="ACG8PN_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS4861682.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACG8PN_001536"
     CDS             12690..12815
                     /locus_tag="ACG8PN_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861683.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACG8PN_001537"
     CDS             12827..13138
                     /locus_tag="ACG8PN_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4861684.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACG8PN_001538"
     CDS             13135..13380
                     /locus_tag="ACG8PN_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861685.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACG8PN_001539"
     CDS             13377..13793
                     /locus_tag="ACG8PN_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4861686.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACG8PN_001540"
     CDS             13790..14128
                     /locus_tag="ACG8PN_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861687.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACG8PN_001541"
     CDS             14125..14355
                     /locus_tag="ACG8PN_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861688.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACG8PN_001542"
     CDS             14362..14565
                     /locus_tag="ACG8PN_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS4861689.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACG8PN_001543"
     CDS             14562..14858
                     /locus_tag="ACG8PN_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861690.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACG8PN_001544"
     CDS             14935..15348
                     /locus_tag="ACG8PN_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4861691.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACG8PN_001545"
     CDS             15361..15747
                     /locus_tag="ACG8PN_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861692.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACG8PN_001546"
     CDS             16298..16525
                     /locus_tag="ACG8PN_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861693.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACG8PN_001547"
     CDS             16522..>16714
                     /locus_tag="ACG8PN_001547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861694.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAZUKA010000060        16676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000060 DAZUKA010000000
VERSION     DAZUKA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.060"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..686)
                     /locus_tag="ACG8PN_001548"
     CDS             complement(<1..686)
                     /locus_tag="ACG8PN_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4861695.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFV"
     gene            861..2285
                     /locus_tag="ACG8PN_001549"
     CDS             861..2285
                     /locus_tag="ACG8PN_001549"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4861696.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2584..3291
                     /locus_tag="ACG8PN_001550"
     CDS             2584..3291
                     /locus_tag="ACG8PN_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4861697.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3278..3601
                     /locus_tag="ACG8PN_001551"
     CDS             3278..3601
                     /locus_tag="ACG8PN_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4861698.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3690..4727)
                     /locus_tag="ACG8PN_001552"
     CDS             complement(3690..4727)
                     /locus_tag="ACG8PN_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4861699.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5048..6031)
                     /locus_tag="ACG8PN_001553"
     CDS             complement(5048..6031)
                     /locus_tag="ACG8PN_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4861700.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6446..7216)
                     /locus_tag="ACG8PN_001554"
     CDS             complement(6446..7216)
                     /locus_tag="ACG8PN_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861701.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7209..8144)
                     /locus_tag="ACG8PN_001555"
     CDS             complement(7209..8144)
                     /locus_tag="ACG8PN_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861702.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8460..10109)
                     /locus_tag="ACG8PN_001556"
     CDS             complement(8460..10109)
                     /locus_tag="ACG8PN_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4861703.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10226..12829)
                     /locus_tag="ACG8PN_001557"
     CDS             complement(10226..12829)
                     /locus_tag="ACG8PN_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4861704.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12841..13890)
                     /locus_tag="ACG8PN_001558"
     CDS             complement(12841..13890)
                     /locus_tag="ACG8PN_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4861705.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13916..14230)
                     /locus_tag="ACG8PN_001559"
     CDS             complement(13916..14230)
                     /locus_tag="ACG8PN_001559"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4861706.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14246..14692)
                     /locus_tag="ACG8PN_001560"
     CDS             complement(14246..14692)
                     /locus_tag="ACG8PN_001560"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4861707.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14693..16615)
                     /locus_tag="ACG8PN_001561"
     CDS             complement(14693..16615)
                     /locus_tag="ACG8PN_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4861708.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4936 a         3396 c         2751 g         5593 t
ORIGIN      
        1 acaaagtctt gatataaatg tctcgatacc gaatactcga aaggtttacc agacgataga
       61 gcaacaactt tgtctatctc aatcataaat tcacctggat ctatttttaa tatttttgcg
      121 tcaaattcat tcgcagtatc tcctttgata cgtatgtgcg aagtctttat ttccagaccc
      181 agttcatttg tgatataagc atatacagat ttctttaaat gttttggttc caagcctggt
      241 atgactgcta atggcatgaa tgtttgctca atcgagtatg gttgacaatc aataaaacgg
      301 cttcgtacaa tattatatac atagtctttt acactgatac ttaatttatc agctactttt
      361 ttcgatggaa gttcaattga gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc
      421 acgctaaaca aaccttcgtt tggaccaaga actcgtgagt tatcaattgg tgttctgacg
      481 acttcagtcc catacccact tctacttcgc aaaactcctt cggaaacaag taagtctacg
      541 cctttcttta ctgttaactt gcttgtatga taaataactg ccaattcatc tcctgtagga
      601 agtttctcac caattttata ctctccattt acaatttttt tctttatatc taagtaaatt
      661 tccatatact tcgcaatttt tttcaatggt gaccctccaa tcaaaaacat atcttcatct
      721 atactttaac ataaataaac cgataaaatc cacatcaaaa ttatacacac aataaaaaag
      781 atatatatat ttaaacaaaa ctatttactt taagttgtaa gcggtgttat cattaagtgg
      841 aaacaggagg taaaaaaatt atgacaacta tgaaaaagct aacttttcca gatgattttt
      901 ggtggggttc cgcttggtca gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag
      961 agactatctg ggatcgctgg tttaaagaac aaccttaccg gttttatcaa cgaatcgatt
     1021 cctctattac aaccgatcac atccatcaat ataaagaaga tatagaacta atgaaaaaaa
     1081 ctgggcataa ctcctttcgc gtttctattt cttgggcacg aatgtttcca aatggcggta
     1141 ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa attattttcc gaaatgaata
     1201 ataacggaat caaagtattt gccaacttat atcattttga catgcctgct actcttcaag
     1261 ataaaggagg atgggaatca cgtgaagtag ttgatgctta tgttcattac gctgaaactt
     1321 gttttaaaga atttggtgac ttagtttgtc attggtttac cttcaatgaa ccacttgggc
     1381 ctattttagg tacttatctt gaagattttc attatccaaa cctcattgac tttaaacgcg
     1441 gagcgcaagc agcattcttc actatacttg cacacgcaaa agcaatacaa gtatttaaag
     1501 aactagctct tcctagtaaa ataggcgtta ttctaaattt gagtcctact tttcctcgta
     1561 gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga tttattttat acgcggagct
     1621 ttttagatcc tatggttaaa gggaattttt ctaaaaagct tatcctgtta ttacaagaat
     1681 ataatcagct tcctgaagat tatacgaaag aagatcttgt atgtatctac gaaaataccg
     1741 ctcaaattct tggactaaac tattacgagc cacgtcgcgt caaatctcgt tcttctatgg
     1801 tggatacatc tagtccattt ttaccagagt ggtttttcga accacatatc atgcctggac
     1861 gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa aggaatttat gatttgtgca
     1921 tggatatcaa gaaccattat ggaaacatcg aagcatttat ctcggagaac ggaatgggcg
     1981 ttgcggatga agaacgtttc atggatgata cagggcaaat aattgatgat tatcggattg
     2041 agtatatcaa agatcatctt gcttacttat ggaaggcaat aaatgaagga tgtaacatta
     2101 aggggtatca tctttggaca tttattgatt gctggtcatg gatcaacgcc tataaaaacc
     2161 gatatgggct ggtttcactc gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg
     2221 aattttacaa gcaattaagt gatacaaatg gatttagata cgatgtagac ttgttagttg
     2281 aataatattc taaaattcca cttgaagtga acacattaga aaacaagaat aatgagaaat
     2341 accaataaca aatacttttt cttcatactt catttatact tgtcagtata agagactgtg
     2401 acaaatgtag tgatcctcag aagttaggtc tggaaatcta acttgagaag atttttttaa
     2461 actacgtcca gtctttttta tttgtttaaa attgaagttg acaagtcttc attaaaaatt
     2521 tagaatattc acaagtttga cgacaattat gaaaatttat gtcgtgctaa aggagttttg
     2581 ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttt
     2641 ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg gtttcatccg
     2701 ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt tatcacggtc
     2761 agcatgcttg ctagccatag tccactgctc tccatcgttc tctcgacctt tctagtcaat
     2821 tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt
     2881 caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa
     2941 ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct
     3001 tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat
     3061 ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt
     3121 caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact
     3181 ttgattctgg tatatgtcgg actgattttt atcccaagta acttagtgat tttagttgta
     3241 acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt
     3301 gctgactatt ttaggttgta ctttagcaac ttggctttct aggatcctac catttgtctt
     3361 actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt
     3421 cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca
     3481 gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa
     3541 aagcttattg gtgatcgtct tggtaggaat tctttccctt tcgttgattc gcttattata
     3601 aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc
     3661 aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact
     3721 tttcctaata tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc
     3781 atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca
     3841 gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg
     3901 aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc
     3961 tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg tttgatttga
     4021 tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat
     4081 tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt
     4141 ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc
     4201 gctagttgat tcaaaatcga accgacgccc ccagctccgt tgatcactaa gatttttttc
     4261 ccgcggttgg cattttcttc tggagtcaga ccaaattttt caaataacaa ttcataagcg
     4321 gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg aaagattttt tggtgcaaga
     4381 gcgacaatac gttcgtcaac cagttggtat tcttgattgc ttcctgctct tgtcgttgta
     4441 cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcatcacc tacagcaaca
     4501 actttcccga ctccatcaaa ccctaagata cgtaaagcat ttcttatccc gttatcttgg
     4561 cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt
     4621 gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcgac
     4681 aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct
     4741 tagcttcatc aagttggagc atccatacag ttttcatttc aatatatata gtatgctatt
     4801 tgttccactt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
     4861 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttttaaac ggtgttcaat
     4921 caactgaccc tcggtattcg ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg
     4981 ctgttcttat tacttgatgc aggacagctt tttcacgacc tctaattcac ggtgttcaag
     5041 caagaaatca ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct
     5101 ctcgtacttc atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg
     5161 gaaatccttt gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga
     5221 tttctgaata atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt
     5281 ctgtatgttc cctcggttct ttttcaaatg cataaggatt cttgaaaatc ccccggccaa
     5341 tcatgatgcc atctacgcca tatttttcgg ccaattcttc tcctgtctgg cgatctggga
     5401 tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt
     5461 tagggatgac ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga
     5521 cagaaagatt ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca
     5581 tttccgtata gccgatacgc gtcttgacgc tgacaggcaa accgccagct ttagccgctt
     5641 caatcaattg tgccgctact tctgggcgaa ggatcaagcc actgcctttt ccacgttctg
     5701 ctacattagg gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca
     5761 tagcaatgct catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg
     5821 gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca tctggatgac
     5881 agaaactgtc tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta
     5941 cgacatggcg aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct
     6001 ttggcagctc tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca
     6061 gtgaagtcgc aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca
     6121 aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa agactgtgac aaaaatctta
     6181 tcacaattcc ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg taataagtcc
     6241 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga
     6301 acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
     6361 tcaacaaacg acaaaatatg aggaactatt ttcccgtttg tttctctaat actcaggtca
     6421 aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat
     6481 accaagtaac ggcgtacaaa acaacgatga atagcacaag tacgatcatc gatgttgcta
     6541 ctggcacatc gattgttcca aggaatccgt aacggaaacc ggagatcata taaacgatcg
     6601 gattgacttt cgaaacagct tgccaaaatg gcggcaacat agagatggca tagaaaacgc
     6661 cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca tcgtcataag
     6721 actgagcaaa aatcccgttg agcagtcctg ctaacgaaaa caagattgcc gtcatcaata
     6781 atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga
     6841 tcgttacgag agagccgacg agaatactgc gacccactcc gccaatcaca aagccccaaa
     6901 tgatcacatg cgtaggaaca ggcgctacca agatttcttc gatgtttttc tggaattttt
     6961 gagagaagaa ggacgaagaa acgttggcat acgaactagt gatagcagac atcataatca
     7021 aaccaggcac gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgaccaccga
     7081 tcatttttcc gaaaataaca aagtacaagg atgtcgtgat cacaggcgga accagtgttt
     7141 ggacccagat acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa
     7201 aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaaaaac
     7261 agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgtc
     7321 agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgt
     7381 tcatcttcaa atgtacaagc gtatccttca atcacaggtt tggtctcata tggcgctaaa
     7441 tcaaagataa atgtttcaaa ttgcagtttg gatagaagct ctttcatact agtattctcg
     7501 atcagttcac ctgattggat gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa
     7561 taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcggag aaactcccac
     7621 atctcgcgtc tcaattcaat atcgactcct gctgttggtt catccaagat cagcaaacgt
     7681 ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata
     7741 cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt
     7801 ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg
     7861 accgtttcaa agggattgaa attgaactct tgcgggacta atccaatctg ttgctttgct
     7921 cggactagat ccgtatccag atcataaccg aagacattca cttttccaga tgttttattg
     7981 actaaagaag taatgattcc gatcgttgta gacttcccag ctccatttgg tcctaatagc
     8041 gcatagaaat ctccttcttt cacggaaaga tcgattcctc gcaatgcttc gaccccactt
     8101 ccataaacct ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct
     8161 tatagacatg attctttatt gtatcatgga atacagcaga tgaaaaaaca acttggttcc
     8221 agcgaaaaat caggttgctg ggctcaggaa aatctcctaa tgaaagacat caatcggacg
     8281 aagtcagaca accttttcag aaaaaacaaa tcgaagataa aggattacta ttcatatgtg
     8341 caacaaaagg agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta
     8401 taaacggcgg aagcaaaagc caagattttt gatccaataa atactttcga tcacttcgcc
     8461 tatcccaata catgccactg ccgcttttca tcaaataacg cggcttccgt ttttgcggtt
     8521 tctactaaag tctctgggta atccaaaact ttcagcaagg caatcgcatt tcgggtcctt
     8581 gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt ttccttctgt tacttgttct
     8641 tcaaagtgga cattctcaca ataattcttt aaaatctctg tcaattcgat atcatgtgtg
     8701 gcgacaaatg ctaaagatgg atactccgca agccaccgaa cgactgaagc ggaagaagca
     8761 atccgttcga tcgtatttgt cccttttaaa atctcatcta cgaagcagta gcagcgttct
     8821 ttggaagcca cttggtccaa caaacgtttg atcgatttga tttctgaaac aaaataactg
     8881 tcgccttcaa ataaatcatc ttcgattgcc atggaagtca acacatgtcc aggttgcagg
     8941 gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct gatggctata
     9001 cttttgacat acgtagattt tcctgatgca ttggaacccg tgactaatgt attcttctgc
     9061 cagttgaccg gattcactac cgcttctttt aacaatggat gatagatatc tttcgctgtc
     9121 acaccgccct cagtaaactc tggtcggcaa gtgatgggca tataagtacg gtaattcaat
     9181 attgcagcgg ctacttctaa ttctcccagc aattcccaca gcttgatggc ttcagttgaa
     9241 tgcttttcta atttagtgag aacgaaatga taggagataa atggcagcat gaacatgatg
     9301 ttgacatatt caaaaatcaa ctctgcttca ctcccactct tcatacgaaa tgaaaaacct
     9361 aatttaggga tgtctcttaa tggtttagtt tgctgtttca gctcttcttg aagtggtgtc
     9421 ggcagttttg ctagctgatt ggcggctgaa atcgttgaaa ccaaataact catgctattc
     9481 agctctgttt ctagtgtttg tttcttcact agataataga ccgtattgaa tactaaccca
     9541 ccgattgcca aatagattcc tagtacttgt cctaaaagaa gcaaacccaa acccaccagc
     9601 ggaagcaagc caagaaaaat aaataaacct acgcgtccaa tctttttact ttttccatcc
     9661 gctagatatt gttttgcaaa attatgatct ttttttccta aacgagcgaa ttgatattgg
     9721 attttttctc tcagttgagg attttcctga taaaaagcaa tcagtttttc taactgctga
     9781 tcttcaccaa aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc tacactagaa
     9841 taggtactgt tgattccttc gaatacttcg aacatatcca ggtcatacca agtaagatca
     9901 tcgatttcgc tatcccatga acggaacttc ttctccgtga gccatgcttc cttcaagctt
     9961 tcttctttat caaatctcgg ttgataagga attttttccc atttgctacg aactgtttcc
    10021 tttaatttca aacgtgaaaa aatgtctaaa gcaactaata aaataacagc tgcaataata
    10081 attaaaacga taaattctct ttgacccata tccctctccg ttcctttatt tgttagcttt
    10141 agtatagcac tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca aaataaaagg
    10201 tgtttttttc ttgtatatta catatttatc tctcatttat caaaattgac aaataatcat
    10261 agggaggaat tgttgttaat tcttgtttat tctccagtgc attgacaact gtcccaaggg
    10321 aaaggatagc ctcaggtact attttttctt gttctgttgg attagctaag cgaagaacaa
    10381 atccttgttc atgaaaataa gaaggataaa tcgatgatac aattaagtct tcaggtaatt
    10441 caatcaatga aaatttacga ggaacatcaa gttcattttg taatggccaa atcttgttat
    10501 ctaacctgtg aataaataca ttcagtttct gcttttgata agaaatagat tgttctaacc
    10561 gtttttctgc ctgttttgcc actttgaact cattaaatct tccttcttct acattaaatc
    10621 caaaagtgac tttatactca cctatctctt gagctagcgg tgtttccatc atgacatgcc
    10681 cttcatttgt cgtatctcct gaagctcgcc caggtcgcca agcaagatta ggttttccaa
    10741 gttcccctgt tgttgaaaat aaggttatga ttaattgatt ttctatacgt tcatactctt
    10801 ttaaaccatc tgcataaaac gtaaaataat gatcattttc tgccacagag acactcttat
    10861 cgaatatttc taaattaaca ggtttctcta ccatagtttc tgaccaattc ggaggaatga
    10921 catcaggggt attttcaata aatccgtttt gaatttgtgc aattgatttt tcagatttat
    10981 ctaaaacatt aattgctaag cgcatacgat gcgagtaaac agtattatga attttgagtg
    11041 tgccttcaac gatttgactt tctcccagac tgaacgatat ttcataagat atttcttcca
    11101 tttcgggttt ttcagataaa cgatctttta aatctttagg aagttgagct tttccgtaaa
    11161 caactaatgt ttcttgtaaa gaatctttat atactttagc tgtttctaat tttagagata
    11221 gttctgtatc tccttctaat ggtgaataat catacgtatc gccatcattt gctgaatcga
    11281 tcaaatgaac gaaatcaaga tattgtctat ctccaactat caaattcaat tgcccatctt
    11341 taaaagaaag tttatattta ttgttactta tctcagtttt gtctatctcg attgctgttt
    11401 ctaatttttc attatttttc tcaaattcaa caacagtgta tcctagtgct ggcacattta
    11461 catttatttt tatgtcaagt tcataataag ctggttcatc aaaatattcg aaacctgtag
    11521 caatcatccg ataagcattt ttccgttctg gaaaataaac ttctttttca atgatattat
    11581 agggactacc tttgaagtgt attttctttg aatcagctac tacatgcacg attttcgatc
    11641 cactataatt ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt agctttaatg
    11701 catcagccat ccgttttaca ataaggtttt cgattccgtc agctatctca ttagcttctt
    11761 tgattcgatg gaaaatatct tccgcaacat tatctgaaac acagccacca atactatcat
    11821 gagcctggtt ttgcaaaatc tttttccata accgacgaag taggccttta ctgatatgga
    11881 taccatttct ttctgcaatc agcatgagcg gctcaactcg ttttagaatt ttttgttcca
    11941 atttaaaatt ttctattttc atttttgttc ttactgagcc aatagaacga tgaacacgag
    12001 agtaaaccgg ctgacgaagt tcacctttat attcaggtaa ttcttttttg tttctgataa
    12061 cttctacata agaaggataa tctgaaataa tattgtggaa tttactgtat ttattcaact
    12121 tctctacgac ttgatcaaaa tttaaaatca ttgacttttg atctacaccc gaaggtacta
    12181 aaatatcttt gttttttcct tgcatatgaa gcatttcaac tgcttcatcc aacttttctt
    12241 ttgcaaattc aggaatatct ttaattgcgt ctaaggtcat cattccagtc atataaccaa
    12301 atggaaaatt catggcataa acaaatttat ctcccaatcc tttccagata aaataaggag
    12361 atggaaccat cgtttcaaag tttaatcctc gccaacacat gaaattatcg atttctactt
    12421 gatgcaaaag tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa taccctacca
    12481 tcattggatc tccatattta aggatcgtat cactgattcc aatcattaag tttcttaaaa
    12541 ttgattcagc atccactaat aaggcatctg tttgtgcata ccacggtccc acgaatagtt
    12601 gtcctgtttt catcagtcgt tggataacct taagcttttg cggattgatc tccaaatact
    12661 catcgacgat tgaagtttgt ccatctaaac aaaagtttac tttagggttc ctttccaatt
    12721 catctagaac ttcagtaaag acctgatcag acaatacgat ggcatcttca tttgtaaaat
    12781 accattctct gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata tcagattctc
    12841 ctactttcct tcttctactg aatttacttg ttgttctttt ttatacttga tggattttag
    12901 tgcattgacc attaacgctg taaccactcc cccaataaca attgatacta aatataaagg
    12961 tatattcgta acaaaaggaa ttgccataaa ggttgaaagc attgtttctt gagtaacacc
    13021 aaaaaatcca tttagaccac atgctactgc agtgcccacc acgatgcttg gtattactcg
    13081 taaaggatct ttcacggcaa acgggatcgt cccttcagta atccccacaa ggcccataat
    13141 catagctgtc tttgcattat ctcgttccga ttcatcataa agttttttgt ttaaaattgt
    13201 tgctaaaaac attccaaatg gcggaatagc aattcctaat aacatggctg tactaggtcc
    13261 aaagcttccg cttgaaaaag aagcaacccc aaaagcatat gcgattttat tgataggtcc
    13321 tcccatatca aacgccatca ttcctcctaa aatagccgac atcaaagcag catttgctcc
    13381 tgacatgcct tctagccaat tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag
    13441 tacataccat aatatgattc caacaactaa tgtagtaaca aaaggaatca gcataattga
    13501 ttttagacta ataacttgca tcggtacttt tacttttttc aatgcattag ctaaatagcc
    13561 acaagctaat ccagcaacca ttcctccaat aaatcccata ccaagatttt gacacatcat
    13621 cccagtgata aaagctggtg ctagaccggg acggtcagca attgaatatg cgatataggc
    13681 tgaaatcatc ggtacgataa actgtaatcc cgtctgacca atcattcgca ggactcccca
    13741 gaatgtatcc gtctggtccc aaacacctgt gccaccaaaa atgactgcaa tagaagttag
    13801 taaccctgct gctacgacaa gcgggatcat ataggaaacc ccactttgga aatgatttag
    13861 aatttctctt ccttgtttct tgaattccat tagtaactcc cccacgatat attttttatt
    13921 tgttaattaa tgcaattgct tcatcaatcg tctcttcacc atcttttact acctctgagg
    13981 tccctactgt taaaatcggt ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta
    14041 ctgcaaatat gacaacatct gcctcggaaa catccgaaat tgaaagtttg ttttctatcc
    14101 ccatagcccc ctgtgtttct actttaatat ctaacccttt cttcttcgca aacttttcta
    14161 aattagcttt tgccatataa gtatgggcaa tccctgcgat acatgctgtt actgctacta
    14221 tttttgtcat atgaaattcc tccttttaaa atgatagagc ttttaccaat tcttttttat
    14281 ttctcgaatc ttttattctt ttaacaaact cgtcatccat caatcttcca gataattctg
    14341 ctaaaatacg taaatgatta gtacctttat cagtgtcttt tattgctaac aaaaagacga
    14401 gatcaactgg ttgatcatct aatgcgcccc attcaacaga ccgctttaac ttggctacag
    14461 ccactgttga ttcaattact gctaggcttt tgccatgagg aatggcgata tttttcccaa
    14521 ttcctgtggt catatggttt tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag
    14581 acacaaaccc attagtctct aacaatttcg ataatttttc gactacactt tttttatcag
    14641 atgcatttaa atctatcact atattattta tattgatttt ttcactgatc atttataagc
    14701 cttctttcaa tatataaact aatttttcaa acgacgaact cgtttttaaa tttgctaata
    14761 actcatgatt cgaagcgatt tcattgaatg tttcataaat tttattcaat tcaggtttta
    14821 ctttttcatt aagtgcaaaa aagaaaacag aatgaacaag tgtcccatcc caatcaattc
    14881 cttctggatt aacgtagaca cagattactg gcttttttat aaaatctact tcgatatgcg
    14941 gcatagcaaa atcattcatc gctgttgaag atagtttttc tcttttgatt gctccctcta
    15001 gtatcccttc tttcgcgtaa ttttctttta ccaatttttt ggtaattata tttaaaacgt
    15061 tcacttgatt atcctttatt tggtgggtaa aaaagcaaaa tcgtttatct aataaattta
    15121 gaaaagcatc ttctgtttgt tcatttaact gtagatactg ttttactttt ctgacatctg
    15181 cccctaacat caaaggacta acaacaataa caggaaaatt tgtattttca atcggaatcg
    15241 tgcttataac aagttcatca gtgacttttc tatcttgtag ttcattgatg cttagtacat
    15301 cttctatctc aaccatatct gcaaaaccgt tatttattct ttgctctaat aattttgatg
    15361 ttccatatcc gctactacaa actaatatca ctttcgtttt gtcaggtttg taacgatcca
    15421 aaagtgattg aatatggagt gttacgtaag cgatttcatc ttcattaaaa tgtatacaga
    15481 atctctcttc gatttcctct aataaatcta cacttatcat aaatgacctt ttaaaagaat
    15541 atttgatttt gtccgtataa gggttataga tatttactcc caatttgagt cttttaactg
    15601 ctgcattgag atgtaatact agattattga tcaattcttc atcaggataa aattcattta
    15661 atttaattct tactaactct tgtagctcaa cacccatatc taaataggaa agcttcgttt
    15721 gatctaagtc gtgattcata gcattttttt caatcgaagt aatgtgaatg tcaacataag
    15781 cttgttcatc tattggcaca ataattgaaa atgctttttc aatcattttg attaaaataa
    15841 gtgtattctt tgagatttct tcttcagaat tattactacg tgataagaaa ttgcttgttt
    15901 caatcctttc tatcgcaata gcaaggtgga tagctaaagt ttgaaattca taatctgtaa
    15961 atttcaattg tgtcttattc atgtatcgat taagcaattc ggctactttt ttcaatatgt
    16021 ccttatttaa gaaattattc gcttcaccaa tgaaattgat attcaagtct aagagatctg
    16081 ttttactgtt agatgctgta aagccacccc aataatagtt cacaacttct gacatcaacc
    16141 ttcttttact tttttcatca gctttgatgc gcacgccatt tcgatctgaa actaaggaca
    16201 tcccatgctt gtttagtctt tttctaacat ctttcattgt gctttctacc gtcgacctgc
    16261 tcacgtataa ttcgtcagca agattttgaa tctttagaga atcattagta ttcaataatt
    16321 tagcaaacac ataagttaga cggtcctccg gagagtctgc gatattgatt ttatgactgg
    16381 ataataaatt tcgaagtgtt tctttttttc caataagcga aacgcctttt ttaggttgaa
    16441 ccaacatttg tacatctggc attttttgga ttatttcttt caaaattaaa atatctttgc
    16501 taattgtttt cgtagataca tataattgtt ctgataattc tttgtaagtt gtgtgctcat
    16561 tttcaatcaa aaagtatagt attttcattt gccgttcgct aaattcgatg cccattatct
    16621 tcacctcctg aatacagctt aagtgtacaa ttattatctc tattttcata cagaag
//
LOCUS       DAZUKA010000061        16555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000061 DAZUKA010000000
VERSION     DAZUKA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.061"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1133)
                     /gene="rpoD"
                     /locus_tag="ACG8PN_001562"
     CDS             complement(24..1133)
                     /gene="rpoD"
                     /locus_tag="ACG8PN_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4861709.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1157..2998)
                     /gene="dnaG"
                     /locus_tag="ACG8PN_001563"
     CDS             complement(1157..2998)
                     /gene="dnaG"
                     /locus_tag="ACG8PN_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4861710.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3180..5495)
                     /locus_tag="ACG8PN_001564"
     CDS             complement(3180..5495)
                     /locus_tag="ACG8PN_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4861711.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5680..6540)
                     /locus_tag="ACG8PN_001565"
     CDS             complement(5680..6540)
                     /locus_tag="ACG8PN_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4861712.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6599..7231)
                     /locus_tag="ACG8PN_001566"
                     /pseudo
     CDS             complement(6599..7231)
                     /locus_tag="ACG8PN_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7398..8159
                     /locus_tag="ACG8PN_001567"
     CDS             7398..8159
                     /locus_tag="ACG8PN_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4861713.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8185..10776
                     /gene="mprF"
                     /locus_tag="ACG8PN_001568"
     CDS             8185..10776
                     /gene="mprF"
                     /locus_tag="ACG8PN_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4861714.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10964..12151)
                     /gene="tuf"
                     /locus_tag="ACG8PN_001569"
     CDS             complement(10964..12151)
                     /gene="tuf"
                     /locus_tag="ACG8PN_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4861715.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12519..12875
                     /locus_tag="ACG8PN_001570"
     CDS             12519..12875
                     /locus_tag="ACG8PN_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4861716.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13116..14120)
                     /locus_tag="ACG8PN_001571"
     CDS             complement(13116..14120)
                     /locus_tag="ACG8PN_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4861717.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14286..15137
                     /locus_tag="ACG8PN_001572"
     CDS             14286..15137
                     /locus_tag="ACG8PN_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4861718.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15154..15399
                     /locus_tag="ACG8PN_001573"
     CDS             15154..15399
                     /locus_tag="ACG8PN_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861719.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            15586..16290
                     /locus_tag="ACG8PN_001574"
     CDS             15586..16290
                     /locus_tag="ACG8PN_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861720.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         4881 a         3408 c         2929 g         5337 t
ORIGIN      
        1 ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa
       61 ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca
      121 ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt
      181 aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct
      241 tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa
      301 tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc
      361 gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc accgatttct
      421 tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta
      481 ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct
      541 cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg
      601 tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg
      661 aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct
      721 tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact
      781 tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg
      841 tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt
      901 ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata
      961 cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt
     1021 gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg
     1081 atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa
     1141 ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa
     1201 tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt
     1261 tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga
     1321 tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc
     1381 ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg
     1441 agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt
     1501 ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct
     1561 gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa
     1621 cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat
     1681 cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata
     1741 gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga
     1801 ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt
     1861 taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat
     1921 acttacggag atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta
     1981 atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt
     2041 ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag
     2101 ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa
     2161 tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg
     2221 ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca
     2281 agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac
     2341 cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag
     2401 acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa
     2461 ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt
     2521 tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct aaagctggct
     2581 gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag
     2641 caatcagttt ccccgtagca gattcaggat tttggacagc tatagtacgc tgacgccatt
     2701 gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta
     2761 gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat
     2821 gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac
     2881 acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa
     2941 cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt
     3001 tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa
     3061 ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt
     3121 ttgattgtat catatttcat tgctttttca agtataacac atttctatct cattaagcat
     3181 caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga
     3241 agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag
     3301 tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt
     3361 actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa
     3421 ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat
     3481 gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa
     3541 gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa
     3601 gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg
     3661 ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc
     3721 gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca
     3781 aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga
     3841 cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa
     3901 acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt
     3961 accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct
     4021 tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt
     4081 aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc
     4141 agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga
     4201 tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc
     4261 tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga
     4321 ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc
     4381 agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc
     4441 acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta
     4501 tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga
     4561 tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc
     4621 aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt
     4681 cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa
     4741 ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa
     4801 gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa
     4861 ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga
     4921 gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca
     4981 gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg
     5041 acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata
     5101 acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc
     5161 agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg
     5221 gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata
     5281 acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac
     5341 agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat
     5401 atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt
     5461 ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa
     5521 ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt
     5581 ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt
     5641 ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa
     5701 acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac
     5761 cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac
     5821 tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat
     5881 ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag
     5941 tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt
     6001 agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca
     6061 tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa
     6121 ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt
     6181 ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa
     6241 tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc
     6301 tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt
     6361 aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc
     6421 accgctgact cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa
     6481 catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac
     6541 tatttgcgta ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt
     6601 acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca atatcaggat
     6661 aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag gactggatcc
     6721 gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc acatctttag
     6781 aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca taggcaatat
     6841 aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct tgtttctttg
     6901 cttctactat catttccgcc aaatatttga caccttgatc aatactgtca gctggattat
     6961 caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct gtcccttttc
     7021 cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc atatgataat
     7081 tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc gaacgataag
     7141 ttcttcctca taaaaacaac accgcaccaa taaccgtcag aatgatcatg taaaaaagaa
     7201 tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt gacatctttt
     7261 tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg tgtacgcctt
     7321 aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg ttgtttttca
     7381 gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta ggtgattatg
     7441 tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga
     7501 gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc cgcttttttt
     7561 cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat agccgaggac
     7621 atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc gaggatctga
     7681 atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt agtgatggag
     7741 caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga ttgattttaa
     7801 attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc agtaatattc
     7861 attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa aatcttttag
     7921 ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact
     7981 cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata
     8041 tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga catgaattag
     8101 ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct gaaacataac
     8161 tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct gaaaaaccac
     8221 ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt cggtgaattg
     8281 atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc cactatacct
     8341 gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc tatggttggt
     8401 tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta cctgtttgag
     8461 acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt tgttagtgtg
     8521 gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat ccaagcatta
     8581 tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct ttctttttta
     8641 cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg gttgatcggc
     8701 ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa aggattcctt
     8761 ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt ggaatggacc
     8821 ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat tgatctgtgg
     8881 caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat gattccagga
     8941 gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg tgtgccaaga
     9001 gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat tccgtttttg
     9061 attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg ttattctggt
     9121 atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt attatacttt
     9181 tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga gtttcgctgg
     9241 cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag tatactattg
     9301 ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg agcatatttg
     9361 cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga ttttagtttc
     9421 acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa aaacgaatta
     9481 tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat cattattggt
     9541 gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt ccctcatcgg
     9601 cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt ttcgggattg
     9661 ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct tcaaggagaa
     9721 aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct tacaacttat
     9781 ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt tgcctatgaa
     9841 aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa atgtattgta
     9901 atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc ttttatcgaa
     9961 gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga gattgttatg
    10021 attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta tgtagacctg
    10081 aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc
    10141 gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga gcaaatgagt
    10201 actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg gttttcttta
    10261 ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa aaatacccaa
    10321 gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa agtaggaacg
    10381 attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc
    10441 ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt agggatggcc
    10501 cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat tgcatacttg
    10561 gtttacgagt ttggttctca tttttattct ttccatggat taagagaata taaaagcaag
    10621 tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg gattgcatat
    10681 gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca aggtagaata
    10741 agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga tagatgaaca
    10801 ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct acaatctctt
    10861 cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga ccgctctttt
    10921 attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg
    10981 taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt
    11041 caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca ggcatgacca
    11101 tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg aagtagaact
    11161 gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt gtcaacacgt
    11221 acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca agtacttgtc
    11281 cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta tccccagctt
    11341 ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct ttttgtgttt
    11401 caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca cgatcaatac
    11461 gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact ggcaatagta
    11521 atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta tccatcaatt
    11581 ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg gaacctttga
    11641 tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga acttccattt
    11701 ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag aaaacaatca
    11761 ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga ggcatcggac
    11821 cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct gtaatcatgt
    11881 ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt ttttctgttt
    11941 catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct ggagctgcat
    12001 caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact gtagtgatcg
    12061 cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg
    12121 gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa taatatagat
    12181 cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta aatcaaaaag
    12241 aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt caatattggt
    12301 caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt ttataaataa
    12361 aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt gaagcaataa
    12421 gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa tagacgaaat
    12481 cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta attggcgtac
    12541 ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat ttttacggcg
    12601 gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc caagcagata
    12661 acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa gatggtgaaa
    12721 aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat gcgtatctta
    12781 aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag gcttctgaag
    12841 tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa ttttattaga
    12901 taaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    12961 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtctcacgc
    13021 tctttttttg ttatattcgt cttttccgat ccttctcttg acacaattca tttaactgat
    13081 cactggtact tgaacaccta atattctagt catttttata aacgattcaa tacttcatcc
    13141 acatttttga tcatcacgga tatcgtacca tccggattat tcacaaattc aatcaaatct
    13201 gggttgcgat atacatctac tggtacgatc agttcgatcc cgtttgatag cttgaatttt
    13261 tgttttccaa attttttctc agaaatttct tgtacttctt ttacaggagg tgcttctggg
    13321 acaaatcctt gctcttgtac ttcttcttta aaagccattt ttgccgtgat gttttccttg
    13381 aagacttttt ccgcaatcat ctcatgatcc aaccgacctt tttcttctat cgtatcatag
    13441 acagcttctt tcaaatcagc catgatatca aattcttctg tctcaaattt ttttccaaga
    13501 tgcttggcta ctttttggat cacttttaca ttttcttcta aagaaggtgc aggaacactt
    13561 tcgatcactc ttccagaaaa ataaagctct tttttgccag aaaactcata ttttttttca
    13621 attaattcgt aggtccaatc agtcaaattc acagcgaatg cttcatctgc tttttgtgtt
    13681 ttcgccccta gaattgctcg attcaaaatc aagcggttgt cgatccctgc ttcgtctgct
    13741 tcaacaaagt gagtgtaagc ctcacgataa ttgactttca ataaagataa gtacatcact
    13801 gtatctaatt catacaagac aacaaataca tctgcactag gtgcttcttc actttcttgg
    13861 tagatatcga accaccgctg aacgattgct tcacttgctt ccacaaaatt ttcttgagcg
    13921 attttaagtg attcaacgat catgctttct tctgctagtt gtccagtttt tgtttgagca
    13981 gaagagattt tttggatctt cttttgaaga aaatcacgaa tatattcttt tgtcagatcc
    14041 agctcttttt gtgaaaagac tggagaacca gtttctctgt caataatatg cagaattgct
    14101 ttttttaaat agatatccat tttctcattc ccttctgtct tagtttacgg aaaaaaaatc
    14161 aaaaatacca gaatcaaaat cattttcttt taaacaaaaa tttcaagagt agaaacaaaa
    14221 tggattcttt ttttcgaaaa atgcgctaca cttatagttg aacgaataaa aaaggagctg
    14281 aaagtatgaa ttcattaaca agcacttatc gtctatcaaa tggttacgaa atcccggtag
    14341 taggattcgg cacatggcaa acacctgatg gggatgtcgc tgtttcctct gttaaagaag
    14401 cgctggctgc aggttatcgc catatcgata ctgcccaagg atacaagaat gaagaaagcg
    14461 tcggccaagc gattaaggaa agtggaattc ctagagaaga gatattcttg acaacaaaat
    14521 tatggaatgc aaaccacagc tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga
    14581 agctccaaac agattattta gatctgtttt tgatccactg gccaaaccct gttgcttttc
    14641 gtgataattg ggaacaagca aatgcagata catggcgagc atttgaagaa ctgtatgaag
    14701 caggtaaaat caaagcaatc ggtgtgagca atttcttacc ccaccatcta gatacactag
    14761 caaaaacagc aaaaattatg ccgatggtca atcaagtttt ccttgcacca ggcgaattac
    14821 agacaactgt agttgagtat gcaaaaaaac atgatatgat cctcgaagca tatagtccat
    14881 taggcactgg taaaatcttc gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca
    14941 agacgattgc acaagttgct ttgcgttggt cgctgcaaca cgaattcctt cctcttccta
    15001 agtcagtgac accaagcaga atcaaagaaa atactgaatt atttgatttt gaattaacag
    15061 aagaagaaat gaaacaaatc gatcagttag atggtgtagt cggcaaagca aaagacccag
    15121 atactaccca attttaagtc gaaaggtgat tttatggatc caatcgaacg cttgaattct
    15181 ctctcggaag aagttaccca gacttttcat tccgattttg tctttttgat cgatgcagag
    15241 aaaatccaac attttcctgc aagaaactgg acacatgatc aaatcatcga agaactgaaa
    15301 aaacggttcg atcattcgct aatggtcaaa ccctggcacg aacatgaagt catttattca
    15361 cctgaactac ctgtttttgc cttgataccg aaaaaataga aagaatggaa cggattatgt
    15421 atatatacat aatccgttcc attttttatt tttctttcaa aaaacgggaa aatacgcaaa
    15481 tttttctgtt gtaatttgcg tatacgcaag tttttttgca ttactccttt tcatttggct
    15541 gtttttttct tataatcgaa agggtatgag cgaaggagag aacatatgaa gaaaaaacag
    15601 aaacaaaaat tattaaatca attccgtcct tcattggatg gtgtaagagg acaattattc
    15661 cgttctctag aagaagagag cttgcgacgt tatggtttgc cgttacaagt catgcttgat
    15721 cccaaaaacc gtcaaatctt aacgatcgga ttagctggga aaacaaatga agatacacgg
    15781 atcaacgtgc caatcgatga caacttcaat actgtattaa agagaattcg ttcaggagaa
    15841 aacgggattt tcgaacgatt ccgtgacaat cttcttattg aaatcgtgtc ttactggaat
    15901 gatcaacgat tgaaaaacga tgaaccaact gcgcaagctg ttgcgacacc agttacagaa
    15961 gaaacagtca gtgtgccaga agaaaaaacg gaaaaaccaa aagcggacac aactgaaaat
    16021 acacgctcaa ctgatacgaa tgccgcttta agttttgctg atttttcaaa agccgttgct
    16081 gagtatccta aattttacac agaacaaaat gaacatgaag tagttattaa tgaaaaaaat
    16141 gctgatgaac ctcgtaaatt agcaactatt tctatgacta ctgaaaatga gttcgttatc
    16201 gaaaaagcac tcgaacggaa atacaaagtc aaattgactt tgattccttt gatcgaacaa
    16261 tttgcagcga cagcgattac tgatcgataa aaattctatt tgtcataaaa ggctgggatt
    16321 taaaatccca gcctttttag tcatataata atttctacag caagtaaaaa gaaattcctt
    16381 ctaacatcag gctcaaatat cagcttgacc tgtccaacaa atcacctatg atcctacaat
    16441 gttaggcgac ttctcttcta cctgaagata tcttggagtt gcctgatttc ttaagatgcc
    16501 taaccttaac tataaaaaat tctaataata aacatatttt ctgatagaga aaaaa
//
LOCUS       DAZUKA010000062        16351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000062 DAZUKA010000000
VERSION     DAZUKA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.062"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(219..1946)
                     /locus_tag="ACG8PN_001575"
     CDS             complement(219..1946)
                     /locus_tag="ACG8PN_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4861721.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1939..3132)
                     /locus_tag="ACG8PN_001576"
     CDS             complement(1939..3132)
                     /locus_tag="ACG8PN_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4861722.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3319..3963)
                     /locus_tag="ACG8PN_001577"
     CDS             complement(3319..3963)
                     /locus_tag="ACG8PN_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4861723.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4057..4191)
                     /locus_tag="ACG8PN_001578"
     CDS             complement(4057..4191)
                     /locus_tag="ACG8PN_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4861724.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4193..6325)
                     /gene="feoB"
                     /locus_tag="ACG8PN_001579"
     CDS             complement(4193..6325)
                     /gene="feoB"
                     /locus_tag="ACG8PN_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4861725.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6322..6795)
                     /locus_tag="ACG8PN_001580"
     CDS             complement(6322..6795)
                     /locus_tag="ACG8PN_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4861726.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7196..7420
                     /gene="nrdH"
                     /locus_tag="ACG8PN_001581"
     CDS             7196..7420
                     /gene="nrdH"
                     /locus_tag="ACG8PN_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4861727.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7579..9738
                     /gene="nrdE"
                     /locus_tag="ACG8PN_001582"
     CDS             7579..9738
                     /gene="nrdE"
                     /locus_tag="ACG8PN_001582"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4861728.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9788..10753
                     /gene="nrdF"
                     /locus_tag="ACG8PN_001583"
     CDS             9788..10753
                     /gene="nrdF"
                     /locus_tag="ACG8PN_001583"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4861729.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10842..11981)
                     /locus_tag="ACG8PN_001584"
     CDS             complement(10842..11981)
                     /locus_tag="ACG8PN_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4861730.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12290..13003)
                     /locus_tag="ACG8PN_001585"
     CDS             complement(12290..13003)
                     /locus_tag="ACG8PN_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4861731.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13257..13958)
                     /gene="nagB"
                     /locus_tag="ACG8PN_001586"
     CDS             complement(13257..13958)
                     /gene="nagB"
                     /locus_tag="ACG8PN_001586"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4861732.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14192..15724
                     /locus_tag="ACG8PN_001587"
     CDS             14192..15724
                     /locus_tag="ACG8PN_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4861733.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15915..16094)
                     /locus_tag="ACG8PN_001588"
                     /pseudo
     CDS             complement(15915..16094)
                     /locus_tag="ACG8PN_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16114..>16351)
                     /locus_tag="ACG8PN_001589"
     CDS             complement(16114..>16351)
                     /locus_tag="ACG8PN_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4861734.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5054 a         3381 c         2864 g         5052 t
ORIGIN      
        1 taataaactt taatcacatt agtctttcat acctacgatc gatctctttg actcgcaccc
       61 ataaattcat aatagtttgt cggcgtcttt gaacgtttcg aagatgagct acgcattctc
      121 tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg gatatttgat
      181 ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca gctttcgact
      241 aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt tccattgttg
      301 atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc taatactttg
      361 taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat ctgctcttcg
      421 cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc aaacatccaa
      481 tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg acctagtgca
      541 acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt gtcatatttt
      601 tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc tatgattttc
      661 ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc tggcactgct
      721 aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc tttatattgt
      781 gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc tgactcgcca
      841 tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac atcatagccc
      901 atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc ccattcaaca
      961 taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat accaatcgca
     1021 cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt acttttctgt
     1081 gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt aaaacggtca
     1141 atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc gacttgtgct
     1201 ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat agaggcaata
     1261 acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat cgtactttta
     1321 gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa
     1381 gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg aacggttggt
     1441 ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc gaaaaatgca
     1501 actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag gatcggtttg
     1561 ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac cccaatgatg
     1621 atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc attccataga
     1681 ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc
     1741 ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc tggaatcact
     1801 aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat
     1861 aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt tgccactggt
     1921 aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg ctaatgcttc
     1981 aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa ctgcaagtgg
     2041 agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt tcccaatcac
     2101 cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag cttctaacgc
     2161 ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca tcagcatgct
     2221 gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt
     2281 cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat cgacttcttc
     2341 cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac ctgttccgat
     2401 ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta aagcttcatt
     2461 caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt ccagtaattc
     2521 gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt ccatcagcaa
     2581 gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca tcccaccaga
     2641 taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta atgctttttc
     2701 tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt attctgtatt
     2761 ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat tgattttatg
     2821 acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc catcaatata
     2881 gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg tggatttccc
     2941 actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa aattcacgtc
     3001 atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct ggtgatcatt
     3061 catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat gttttacttc
     3121 tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc tgaaaaaagt
     3181 gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc
     3241 caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa gttaatattt
     3301 ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat
     3361 acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg taaatttagc
     3421 ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa ctgtggcgcc
     3481 agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg ctttttctga
     3541 taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg tttgattgac
     3601 cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct ccacacttgc
     3661 catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca ataggaaatc
     3721 ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt ccatgattcc
     3781 aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat tgtacgtact
     3841 ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt caacgatacg
     3901 ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt tctttccaga
     3961 cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct agcaaaataa
     4021 gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct gttccttttt
     4081 tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac gagtatagac
     4141 aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca tcctattcct
     4201 ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga cggaacaaaa
     4261 cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct acctgtccac
     4321 cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct aaaccgcatt
     4381 gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg atagccccaa
     4441 ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa gctgctgcgg
     4501 gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat acttccgcat
     4561 gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat cctgtaatcg
     4621 ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact gccaggattc
     4681 gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa aaattataag
     4741 aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct tttttaacaa
     4801 aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga aaatgatatg
     4861 cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt tttttcaagc
     4921 tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga gctacccaag
     4981 attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt gcagaacaag
     5041 gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca atcgtgcgac
     5101 tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg aaggattttc
     5161 ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc gccatatatc
     5221 cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt agaaatccta
     5281 acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc catcccgcta
     5341 cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca aacaatgtgt
     5401 cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa tagatcagcc
     5461 acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc tgatcgattt
     5521 tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta catagttggg
     5581 taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc aatagtttct
     5641 ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc tcgtaatcct
     5701 ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc ttgccgcttt
     5761 gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga tcatatgtac
     5821 agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg attagttttt
     5881 cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga ccataggaaa
     5941 gcttttctag attgattttt tttccttctt ttttgatgac atccatcata ttcagaccaa
     6001 tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt cgttctaaat
     6061 tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa taatctcttg
     6121 caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg tcctgaatat
     6181 gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg cccggccaat
     6241 tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc ccgctgtttg
     6301 gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga ttagttcggc
     6361 ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg gatctcccag
     6421 aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat ccattaaccg
     6481 tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc ctgcttttaa
     6541 ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa agatttgacc
     6601 tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg cattgttgcc
     6661 tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt gcaaatggcg
     6721 ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct gctttgcctg
     6781 cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta aaatcattca
     6841 ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc tcacatagaa
     6901 tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa tctttagtat
     6961 tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt tttctctatt
     7021 aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa acacaaaact
     7081 tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact tttttactaa
     7141 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
     7201 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
     7261 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
     7321 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
     7381 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
     7441 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
     7501 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
     7561 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
     7621 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
     7681 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttcc atctattatg
     7741 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
     7801 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
     7861 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
     7921 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
     7981 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
     8041 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
     8101 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
     8161 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
     8221 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
     8281 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
     8341 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
     8401 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
     8461 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
     8521 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
     8581 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
     8641 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
     8701 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
     8761 aacggcaaac aggaatatga agtattagga acagatatct catgtaactt aggctcgaca
     8821 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
     8881 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
     8941 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
     9001 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
     9061 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
     9121 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
     9181 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
     9241 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
     9301 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
     9361 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
     9421 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
     9481 atgcgtaaag taatcgatgt ttatgcaacg gcacaacaac acgtcgatca aggcatgagt
     9541 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
     9601 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
     9661 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
     9721 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
     9781 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
     9841 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
     9901 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
     9961 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
    10021 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
    10081 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
    10141 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
    10201 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
    10261 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
    10321 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
    10381 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
    10441 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
    10501 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
    10561 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
    10621 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
    10681 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
    10741 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
    10801 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
    10861 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
    10921 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
    10981 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
    11041 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
    11101 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
    11161 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
    11221 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
    11281 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
    11341 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
    11401 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
    11461 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
    11521 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
    11581 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
    11641 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
    11701 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
    11761 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
    11821 ataaaaccag caacaagcaa tggtaaaaaa attgccgaaa gcattttcaa tattggacta
    11881 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
    11941 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
    12001 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
    12061 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
    12121 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
    12181 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
    12241 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
    12301 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
    12361 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
    12421 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
    12481 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
    12541 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
    12601 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
    12661 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
    12721 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
    12781 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
    12841 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
    12901 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
    12961 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
    13021 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
    13081 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
    13141 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
    13201 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
    13261 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
    13321 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
    13381 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
    13441 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
    13501 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
    13561 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
    13621 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
    13681 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
    13741 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
    13801 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
    13861 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
    13921 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
    13981 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
    14041 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
    14101 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
    14161 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
    14221 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
    14281 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
    14341 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
    14401 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
    14461 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
    14521 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
    14581 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
    14641 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
    14701 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
    14761 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
    14821 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
    14881 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
    14941 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
    15001 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
    15061 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
    15121 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
    15181 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
    15241 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
    15301 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
    15361 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
    15421 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
    15481 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
    15541 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
    15601 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
    15661 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
    15721 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
    15781 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
    15841 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
    15901 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
    15961 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
    16021 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
    16081 aacataaata cagacccgtg gtgctagtta attttaatat cctaaagaat atctcaaagt
    16141 tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg taagccagta atatgctttc
    16201 aaatctactc tctagccctt taacagaacg tgagttgaaa ttttgacaac ctaatttttc
    16261 taaggaagaa aaaacagtct caactgtttt tctttgtttt ccaagtaagc tgtggtctag
    16321 tttttctgat ttatccatgt ttttacgtgg c
//
LOCUS       DAZUKA010000063        16319 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000063 DAZUKA010000000
VERSION     DAZUKA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.063"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8PN_001590"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8PN_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4861735.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8PN_001591"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8PN_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4861736.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8PN_001592"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8PN_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4861737.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8PN_001593"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8PN_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861738.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8PN_001594"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8PN_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4861739.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8PN_001595"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8PN_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4861740.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8PN_001596"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8PN_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861741.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8PN_001597"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8PN_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4861742.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG8PN_001598"
     CDS             complement(7139..7417)
                     /locus_tag="ACG8PN_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4861743.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG8PN_001599"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8PN_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4861744.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8PN_001600"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8PN_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS4861745.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8PN_001601"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8PN_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4861746.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8PN_001602"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8PN_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4861747.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8PN_001603"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8PN_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4861748.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8PN_001604"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8PN_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4861749.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8PN_001605"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8PN_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFS4861750.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8PN_001606"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8PN_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861751.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8PN_001607"
     CDS             13967..14695
                     /locus_tag="ACG8PN_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS4861752.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACG8PN_001608"
     CDS             complement(14826..15302)
                     /locus_tag="ACG8PN_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4861753.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACG8PN_001609"
     CDS             complement(15262..15618)
                     /locus_tag="ACG8PN_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861754.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACG8PN_001610"
     CDS             complement(15596..15781)
                     /locus_tag="ACG8PN_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861755.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACG8PN_001611"
     CDS             complement(15782..16054)
                     /locus_tag="ACG8PN_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFS4861756.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACG8PN_001612"
     CDS             complement(16051..16251)
                     /locus_tag="ACG8PN_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861757.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4567 a         3452 c         2536 g         5764 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttatt
//
LOCUS       DAZUKA010000064        16233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000064 DAZUKA010000000
VERSION     DAZUKA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.064"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..1693
                     /locus_tag="ACG8PN_001613"
     CDS             32..1693
                     /locus_tag="ACG8PN_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4861758.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1763..2125)
                     /locus_tag="ACG8PN_001614"
     CDS             complement(1763..2125)
                     /locus_tag="ACG8PN_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4861759.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2193..3134
                     /gene="opp3b"
                     /locus_tag="ACG8PN_001615"
     CDS             2193..3134
                     /gene="opp3b"
                     /locus_tag="ACG8PN_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4861760.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3135..4178
                     /locus_tag="ACG8PN_001616"
     CDS             3135..4178
                     /locus_tag="ACG8PN_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861761.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4184..5254
                     /locus_tag="ACG8PN_001617"
     CDS             4184..5254
                     /locus_tag="ACG8PN_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861762.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5244..6197
                     /locus_tag="ACG8PN_001618"
     CDS             5244..6197
                     /locus_tag="ACG8PN_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861763.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6209..7219
                     /locus_tag="ACG8PN_001619"
     CDS             6209..7219
                     /locus_tag="ACG8PN_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4861764.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7504..8325)
                     /locus_tag="ACG8PN_001620"
     CDS             complement(7504..8325)
                     /locus_tag="ACG8PN_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4861765.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8422..8700)
                     /locus_tag="ACG8PN_001621"
     CDS             complement(8422..8700)
                     /locus_tag="ACG8PN_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861766.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8968..10977
                     /gene="metG"
                     /locus_tag="ACG8PN_001622"
     CDS             8968..10977
                     /gene="metG"
                     /locus_tag="ACG8PN_001622"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4861767.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11456..12043)
                     /locus_tag="ACG8PN_001623"
     CDS             complement(11456..12043)
                     /locus_tag="ACG8PN_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861768.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12218..13540
                     /locus_tag="ACG8PN_001624"
     CDS             12218..13540
                     /locus_tag="ACG8PN_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4861769.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13695..14471
                     /locus_tag="ACG8PN_001625"
     CDS             13695..14471
                     /locus_tag="ACG8PN_001625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4861770.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14464..15033
                     /gene="rnmV"
                     /locus_tag="ACG8PN_001626"
     CDS             14464..15033
                     /gene="rnmV"
                     /locus_tag="ACG8PN_001626"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4861771.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15026..15910
                     /gene="rsmA"
                     /locus_tag="ACG8PN_001627"
     CDS             15026..15910
                     /gene="rsmA"
                     /locus_tag="ACG8PN_001627"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4861772.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
BASE COUNT         5380 a         2918 c         3352 g         4583 t
ORIGIN      
        1 ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt
       61 tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag
      121 ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga
      181 aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa
      241 cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag
      301 cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc
      361 aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac
      421 agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga
      481 agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta
      541 tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc
      601 atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc
      661 aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac
      721 agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact
      781 ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga
      841 tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc
      901 agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa
      961 gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga
     1021 agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc
     1081 agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt
     1141 ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt
     1201 tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga
     1261 atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt
     1321 tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg
     1381 atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc
     1441 atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc
     1501 aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat
     1561 ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga
     1621 aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata
     1681 cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc
     1741 gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa
     1801 gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg
     1861 agagcgtgga aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc
     1921 gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa
     1981 acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga
     2041 tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt
     2101 ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc
     2161 tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa
     2221 aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga
     2281 tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg
     2341 agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc
     2401 tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg
     2461 cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct
     2521 ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg
     2581 cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg
     2641 ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg
     2701 atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact
     2761 ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag
     2821 caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt
     2881 tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg
     2941 tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa
     3001 caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg
     3061 ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag
     3121 ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg
     3181 cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac
     3241 cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta
     3301 tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc
     3361 ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa
     3421 tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg
     3481 ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg
     3541 atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg
     3601 cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac
     3661 gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg
     3721 tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg
     3781 ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag
     3841 atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca
     3901 aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat
     3961 ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag
     4021 cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat
     4081 tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg
     4141 gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga
     4201 agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg
     4261 tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg
     4321 aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc
     4381 tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa
     4441 aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac
     4501 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
     4561 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
     4621 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
     4681 gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga
     4741 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
     4801 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
     4861 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
     4921 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
     4981 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
     5041 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
     5101 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
     5161 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
     5221 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
     5281 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
     5341 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
     5401 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
     5461 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
     5521 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
     5581 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
     5641 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
     5701 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
     5761 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
     5821 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
     5881 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
     5941 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
     6001 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
     6061 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
     6121 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
     6181 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
     6241 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
     6301 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
     6361 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
     6421 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
     6481 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
     6541 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
     6601 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
     6661 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
     6721 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
     6781 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
     6841 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
     6901 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
     6961 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
     7021 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
     7081 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
     7141 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
     7201 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
     7261 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
     7321 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
     7381 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
     7441 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
     7501 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
     7561 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
     7621 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
     7681 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
     7741 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
     7801 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
     7861 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
     7921 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
     7981 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
     8041 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
     8101 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
     8161 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
     8221 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg
     8281 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
     8341 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
     8401 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
     8461 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
     8521 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
     8581 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
     8641 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
     8701 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
     8761 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
     8821 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
     8881 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
     8941 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
     9001 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
     9061 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
     9121 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
     9181 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
     9241 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
     9301 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
     9361 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
     9421 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
     9481 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
     9541 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
     9601 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
     9661 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
     9721 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
     9781 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
     9841 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
     9901 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
     9961 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
    10021 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
    10081 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
    10141 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
    10201 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
    10261 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
    10321 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
    10381 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
    10441 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
    10501 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
    10561 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
    10621 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
    10681 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
    10741 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
    10801 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
    10861 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
    10921 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
    10981 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
    11041 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
    11101 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
    11161 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
    11221 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
    11281 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
    11341 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
    11401 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
    11461 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
    11521 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
    11581 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
    11641 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
    11701 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
    11761 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
    11821 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
    11881 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
    11941 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
    12001 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
    12061 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
    12121 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
    12181 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
    12241 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
    12301 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
    12361 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
    12421 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
    12481 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
    12541 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
    12601 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
    12661 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
    12721 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
    12781 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
    12841 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
    12901 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
    12961 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
    13021 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
    13081 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
    13141 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
    13201 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
    13261 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
    13321 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
    13381 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
    13441 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
    13501 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    13561 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    13621 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    13681 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    13741 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    13801 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    13861 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    13921 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    13981 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    14041 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    14101 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    14161 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    14221 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    14281 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    14341 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    14401 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    14461 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    14521 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    14581 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    14641 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    14701 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    14761 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    14821 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    14881 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    14941 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    15001 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    15061 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    15121 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    15181 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    15241 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    15301 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    15361 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    15421 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    15481 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    15541 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    15601 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    15661 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    15721 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    15781 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    15841 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    15901 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    15961 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
    16021 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    16081 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    16141 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    16201 acatctccta tttttgtttc ttgatcaaaa aat
//
LOCUS       DAZUKA010000065        15992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000065 DAZUKA010000000
VERSION     DAZUKA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.065"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            224..892
                     /locus_tag="ACG8PN_001628"
     CDS             224..892
                     /locus_tag="ACG8PN_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4861773.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1092..1412)
                     /locus_tag="ACG8PN_001629"
     CDS             complement(1092..1412)
                     /locus_tag="ACG8PN_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861774.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1449..2831)
                     /locus_tag="ACG8PN_001630"
     CDS             complement(1449..2831)
                     /locus_tag="ACG8PN_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4861775.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2987..4174)
                     /locus_tag="ACG8PN_001631"
     CDS             complement(2987..4174)
                     /locus_tag="ACG8PN_001631"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4861776.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4181..5212)
                     /locus_tag="ACG8PN_001632"
     CDS             complement(4181..5212)
                     /locus_tag="ACG8PN_001632"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4861777.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5258..6682)
                     /locus_tag="ACG8PN_001633"
     CDS             complement(5258..6682)
                     /locus_tag="ACG8PN_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4861778.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6857..7762
                     /locus_tag="ACG8PN_001634"
     CDS             6857..7762
                     /locus_tag="ACG8PN_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4861779.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7853..8530
                     /locus_tag="ACG8PN_001635"
     CDS             7853..8530
                     /locus_tag="ACG8PN_001635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4861780.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8527..8697
                     /locus_tag="ACG8PN_001636"
     CDS             8527..8697
                     /locus_tag="ACG8PN_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861781.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8811..9707
                     /locus_tag="ACG8PN_001637"
     CDS             8811..9707
                     /locus_tag="ACG8PN_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4861782.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9762..11132)
                     /locus_tag="ACG8PN_001638"
     CDS             complement(9762..11132)
                     /locus_tag="ACG8PN_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4861783.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11370..12116
                     /locus_tag="ACG8PN_001639"
     CDS             11370..12116
                     /locus_tag="ACG8PN_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4861784.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12113..12952
                     /locus_tag="ACG8PN_001640"
     CDS             12113..12952
                     /locus_tag="ACG8PN_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4861785.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12953..13902
                     /locus_tag="ACG8PN_001641"
                     /pseudo
     CDS             12953..13902
                     /locus_tag="ACG8PN_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14043..14795
                     /locus_tag="ACG8PN_001642"
     CDS             14043..14795
                     /locus_tag="ACG8PN_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861786.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14814..15965
                     /gene="queG"
                     /locus_tag="ACG8PN_001643"
     CDS             14814..15965
                     /gene="queG"
                     /locus_tag="ACG8PN_001643"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4861787.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         2995 c         3221 g         4584 t
ORIGIN      
        1 tccaatttcg taaaacaacc gtaacggttg tgtaaaaata tcattttaga gcataaaaaa
       61 aaggagtgga tttctccact ccaaaaaagt ctaacttttg ggggtcacta cagtgtcaca
      121 gtcctttttt tagtaaaagg atacatttct tccttgtata ttccatgcgt gttcgtgaga
      181 aagcatatat aatttaatta acagatagtt agggggagaa aaaatgattt tttcaattac
      241 aatgaaactg gtgttcgggt tgatcggttt attattagtg gtacgtttgc ttgggaagaa
      301 atccatgtca gaaatcacac cgtttgattt agtctatacg cttgttttag gtgggatctt
      361 agaagaatca acctatgatg acaatgtcca tgtagggcat gtcctatttg ccattgcgct
      421 ttgggcagta atgatttatg gtatcgaacg tatcgtccaa aaaaatgaaa aggtcaatcg
      481 ttgggtcaaa ggcgagccat ctgttctaat caaagacggc gtcatcaaca tgacagagtt
      541 gacaaataac catatcgaga tggagcagtt gcgagcaatc ttaagacagc aagaatgctt
      601 ttttcttgaa aatgccaaac atgttatcct agagaatgcc ggtcaaatga gtgtattgaa
      661 aaaatcagat gaagacaaag ctttatcgat cctgcttgta gatgaagggc aaattcagca
      721 taaagtgctt cagtccaatc aattgactga agcatggctt atggagaact tgaagaaaga
      781 aggctatgca gatgtaaaac agctgattta tgttgaatgg tcagaagaaa aagggctcta
      841 catactaaat aaagaagacg tcatgcatga atcttaccga atagatggat agatagacag
      901 aggttgtgaa atcagcgcct tggcttgtta ctcaaaacta agcgactttt tcacaacctc
      961 tgatatctaa tatacgttgt tccagaagtt gcttcttcga aaaataagcc aaaattccac
     1021 aaaaatttaa gaagcaattt tcgaaaaatc ctttcttatt ttttgaagtt gatcacttct
     1081 gtcacaacct ctcatgtcac aaaccatttt tttgtttcct ttaactcaat ataccgtacg
     1141 tcttcccaca atatcggctg atggtcaatc ataaagtaat cttcataggc ttcaccaatg
     1201 aagtgccctt cgacctcatc caagagtctt ccaaattcat cttttaaatt gagctgaatg
     1261 attactgctt tacgagataa aaaggcttct gtcagtatac tatcgatttc ttgcaatgtc
     1321 atttgttgtt taggaagagg ctgtttgcgt gcttcttctt catttttttg ataccgttga
     1381 ctagttcttc catcgcaaaa gcagtccccc attttaatcc aaatggccga tctcgatact
     1441 cgttatagtc aatgaatttt tttgctgtcc gtctcatctt ttccaccttc taatccactc
     1501 ataccacctg catgcccgcc aacgagagaa ctacgtgcga tggcgcgtcc tccttgcaag
     1561 agactatgcg catggatgat tgattgaaag ccgtattttt ttctaatcgt atcgacaaca
     1621 aaatcaactt tcgcttcatt tatctgcttt tcaggatctt caaaaaggtt aagctgtaaa
     1681 cctgtcgcgt atacgaggtc ggaacagttc acgcctacac gccgaatatc ctggtagtgg
     1741 taatgctggt ggaaaatggt caataaagca tctgcaagat ctctgctagc atttgtcgct
     1801 tcgattttca tttgctggtg aaacccggtc ttgccatttg gatcaagata gcctaaagag
     1861 tatcctaccc ataagctgac tacttgtgtt tttgctgcag atcgtctcaa gcgagtgccg
     1921 acttggtcgg ccatttcttt gataacgatc tcgatttctt ctcgtcttgt ataatctcta
     1981 tctaggattt gactatttcc gatacttttt gattttggtg tatattcttg tcccaaaaaa
     2041 cttcgatcga tgccccaagc atgggcataa agctgagtac cgatgacccc tagcttgctt
     2101 ttcaagtgat aataatccgc gtgagctaaa tcataaacag aatgtatacc catttgtttc
     2161 agacgttttg ctgttcgttt gccgattccc caaaagtcag tgatatctga gattttccat
     2221 aacttttctg gtacatcttc ataacgccat tcagcaaaca tttgctttcc gtcttttgag
     2281 ccattgtcta aggctagttt agcaagtagc gggttatcgc caattcctat agtcgtgtag
     2341 attccggtct gctcaaacac atctttatgg atcagtttag ccatgtcgta cgcatttgtt
     2401 ttaccaaaga gtgccatgct atttgttaca tcaaggaagc tttcatcgac ggaatagaca
     2461 tgatggttcg cttcgtctgc atatttttta tagatagaat tgatttctct gttcttttcc
     2521 atataatagg ccatccttgg ggcaacaata tataaatcac ttggataggg gaagggaaga
     2581 tctctggcac ggctgatatt ggtgatgccg taagcttttt ttgctgcagg actggaagcc
     2641 aagattaatc cgctaccccg tgtttctgtc gaatcggacg gataggacat gactactaat
     2701 ttagttgtaa ggggatcaag atccttttca acgcactcta cggaggcgta aaaagatttg
     2761 cagtcaatac ataaaatatc tcttgaaggt tctttttcat aaacaaatac cggattttga
     2821 ttcgcccaca taacaatcac ctcttcaaca caattagaac acacgttctc tttccgtgtc
     2881 aataaaaaac agcaattttc aaatacgagc aaaaggctca catttggaaa ttgctgagag
     2941 gttattttta tctatttgtt tttagctgag atcgttgatt tttttatcaa acatgaacag
     3001 cttctggatc gatctttgtt gtcccatttc caatgtgtgc aacataaaat tccggagcat
     3061 agccgacaac tttcatgtat tcatcataga cattgttttt gaaggcaggt atattttctt
     3121 ctttgacaag agcaatcgca cagccaccaa atccagctcc tgtcattcgt gcacctaaga
     3181 caccttcttg tttttgagca gcatctacta gtgtatctaa ttcgatccca gttacttcat
     3241 aatcatagcg aagagagtgg tgagaatcat ttaacagttg accaaatgct tctaagttcc
     3301 cagcttctaa ttcagctttt gccttcaaag tccgctgatt ttcagaaacg gcatggcgag
     3361 ctctgcgtat caatgtatca tcgccgattt ggtcaatagt tgattcaaat tcttcttcat
     3421 ctaagtctcc taaagaagag ataggcaaag ttttttgcaa acgtgccaag gcttcttcgc
     3481 attcgctacg gcgttcattg tattttgaat ctgccaattc ccgccgctta ttcgtattca
     3541 tgatgacgat tgcataacca tctagtttca ctggaaccat ttcgtatttc agcgtatttg
     3601 tatctaataa gattgcatga tctagctcac cgaagccgat ggcaaactga tccatgatac
     3661 ctgaattgac accgatgaat tcattttcta cttttttacc tgtttggact agttccaagc
     3721 gatctgtttt taaatcaaac agatcttcta atacaacacc tactaataat tctaatgaag
     3781 cgctactgga aagtcctgca ccattaggga ttgttccttc cactaataac tcaaacccat
     3841 gatcgattga gtgtccagct tcttttaatt taaggaccat tccttttacg taatttgccc
     3901 agtttgcttt tttatcatat tctagctcat ctaaagtaaa actaatgaca ccgatatcgt
     3961 caaagtttgt ggaataaact aatattttat tatcgtctcg aggtgctgct acaccttgcg
     4021 ttccataagt gatggaagcg gggaagacat ggcctccatt gtaatctgtg tgttcaccaa
     4081 tcaaattgat gcgtcctggt gagaagtatt gtgtagtttt tttaggtccg aatgtttcgg
     4141 caaattttgt gtttaagctt cttttgattt ccatgatgag ttattccttt cttgttttga
     4201 ttttgaattc tgttactgtt tcaaatactg atccggcttt taaatgaatc gaaccaaatg
     4261 ctgagaagcg ctctgcacct ggagctgttt gagtctcaaa tgtgatgcca ccgtgatgtg
     4321 ccagttttcg gttgcgcatt tctggtgttt ctgtgccgaa atttgcagtg aaaataacca
     4381 cactagaagc atctgtagca atatctactt ggattctttt atcggggcta gtcaagcgag
     4441 cggcttcttt acccaagccg gtttctttta agaaaaatgg atgatcgatt ccgtcaacaa
     4501 gatttttttg atccaaatca ctagcaaaaa cggacgctaa ttttttaggt atttgaaaat
     4561 cgaaagcact tcctgcagca ttttctttta cccctatagg aatactatct gttcgcaaag
     4621 gagcataagc ttcgctattg agccaaagct catgttgatc gattgactga gaagcatctc
     4681 ccgtcaagtt gaagtaaaca tgatttgtcg gattgaataa agtatcttgg tcactggtgc
     4741 cgcgagaggt cactcgccag atattatctt ttgtcaaagt atagcggatt tccacatcca
     4801 ttgtacctgg aaaaccatgt tctccgtcgg gacttgtagt agtaaaaatg acagaagctt
     4861 cattttcgcc attcaaaatg acataagacc atttttttaa ttcaaatcca ggagcaccac
     4921 catgcagatt atgtccagtt tttggatctg tggctaattg gtaagttttt ccattgagag
     4981 aaaagcggcc attttcgatt ctccctgcgg tgcgaccgat agaagcaccg atatacggat
     5041 ctttttcgat atattcttca gcggtgtcaa atcccaaaac taattctcga tcatttgatt
     5101 ttaaactgac gattctagca cctaaatcag agatagaaag tgctagcttg tttttgttct
     5161 ccaaggttat caagtgataa ccttggccaa aatctttttc tgtaatcttc attttcaaga
     5221 tcctttctta atgggaagga actactgcat cgtgaagtta atcttccatt gtatcagctg
     5281 gagccaattt gttttccaat ttctcaacga tttgtgcatg ttttttctct gtcaatgtaa
     5341 tcttccaagc aaaaataata gcggagaaaa tcatcaaagc agcaggagca tagaatgcat
     5401 aagctttgaa agtcgttacg ccatgagcag tgatatcagc agcagtagca ttaccagtca
     5461 tcccagaagc gatagcgaca aaccctacga tcccgttaga caaagcacca gcgattttat
     5521 ctagtaaagg gcgaattgat agagtaacag cttcgttacg gataccattt ttccattgac
     5581 catattcaac agagtctgtg atcgtcatca aagcggataa gaaaatcaat tgttgaggga
     5641 agtagaacaa tactagcccg atgataacga ttggcaaact tgaacctgcg atcgtaaaga
     5701 aaaagtaaga agaaatcatc attgcgatcc ctccaaggta aacgtagcga cgagagatga
     5761 cgcgtgtaag cattgggaat agtggaacag ctactaaacc aagtaaggca gcaactagac
     5821 caacgatcca aaattcagtt ggttggccaa gtacatattt gaagaagtag aataatacgg
     5881 cagttgttgc tgcattggca atcgcataag cgatataagc aagggcaagc cacatcaact
     5941 gatcattttg agcaactgct ttgaacacat ctttgatccc agctttttca ttttgctgac
     6001 ggatcgcagt atcgacttct tttgtgccca aggcagtgat aatcgcagta ccgcctgaaa
     6061 taacagcagc aatcacaccg aaccagaacc agcctgaagc gccttcaata tgtttatgag
     6121 taacaagata ggtaaagaaa gtaacgatag ggacaacgac tagagtagta ccatttgcac
     6181 ctagagaaga gccgaaacga gcaaacgtag caaatttttc acgttccttt gtttccgtac
     6241 tcaaagctgg aatcattgac caaaaagcga tatccttaaa tgaataaaaa ctatccaata
     6301 agatgaaaac gattgcaaat aatatgataa ataaagtcgt attatgagta gctaagccaa
     6361 agaaattggt aaagattgct accaagagta cggaactaac aacgccgccg ataactaacc
     6421 atggtttgaa tttaccgtat tttgtacgag tattatcgat gatgccacca atcaaaggat
     6481 caaaaataat ttcaactaag cggataccta caactaagct tgtaactgta gcaatcattt
     6541 ttgcattcgt tgccttatct gttcctgcga acatctggct tgtcacgaat accataaaat
     6601 aagtgcttaa tgtaacatag aatgtgtcgt gtccaaaagc gcctaaagcg taggacacac
     6661 gttggatcat tttgcttttc ataatctgta ttcctctcct gttcaatata taatactaag
     6721 taaaccggtt acaacgaata acaaagatct tttaatgact taaacttaac aagaaatcgc
     6781 ttccttttta atatatttct gtgactttta tttaactttt tgttgcatta tactaaagga
     6841 gtaaggaggg atagacgtgg agcaatcgat ttttttaaaa aaggaacatc aacaagtaga
     6901 aaaagaattg tattttgatt tttgtggttt ctctaaaacg atgccgtttc attcttttgg
     6961 tcctgctatt cgcaatgatt acatcctgca tgtcgtgatg gaaggcaaag gatcttatca
     7021 tgtgaaaaat caacaattcc aattgaaaaa aggtgatctg ttcttgattc gtccaggaga
     7081 ctcgacgttt tacttatcag atggagaaga tccgtggatg tattgctggc tttcatttgg
     7141 tgggagtgct gcagaagaaa tcattcggca gtcgcttttc agagaagacc aatacacgat
     7201 ggtctcttct gacatacaaa aatatatcga cattatttta gaatgcatga aagatacgga
     7261 agaaaccttg gaaaatgaat tacagttaac agcattaacc taccgttttt tatcagtcct
     7321 tttaaaagat ggcggaaagg tcagcggtgg tgtacaaaag gttcattcct ctttagctgt
     7381 tgaaacagtc aaatacatcg aggcacatta tgctgaaaaa ttgtctgttg aggaaatcgc
     7441 ccagcattta gcggtcaatc ggagccattt gtcgcgagta ttcaaaaatc atatgggtac
     7501 cagtatcaag gaatatttga tcggggtacg gatcaatcga ggggcgtttc ttctttctct
     7561 gacggatgaa tccgtggaaa gtattgccta tcaagtaggg ttcaatagtc tagtcgtatt
     7621 tagccgaatg ttcaaaaaga gtaccggaga aacggctacg aactatcgta aacgtatgaa
     7681 aaacgaaaat tttaagaata ttgctttgga taccttgaaa aaacagttgg aaaaacaaac
     7741 cgcagtttct tggacaacct gattagtctg gttaaaatga caagcatcgg caaaagaaac
     7801 ttgtggcggt tatgctacac tgtaaagcga agactaaaaa ggaggaaaag ggatgcgtac
     7861 acttatcatc attagccatc caacgatcga agaatcttta aatcagcaat ttttcaaaga
     7921 agcttctgct ggtttaccag ctacttggca tcatttggaa tcaagttatc cagacggaaa
     7981 aatagatata gagactgaac tacagctctt ggcccagcat gatcggatca ttttccagtt
     8041 tccgttttac tggtatagtt ctcctgctca tttaaaaatt tggcaagata atgtcttgga
     8101 acgagcagca catgtattaa aagggaaaga actaggaatc gtattgacaa caggtgtggc
     8161 ggaaaaagaa taccaagctg gaggaaaaga agaatatacg atttccgaat ttttgaggcc
     8221 gtatcagcga atcgcaaata aattccatat gacctatctt ccgccattcg ttttagcaca
     8281 atttatgtat ttatcccagg aaaaacgttg ggagaagcta attgcttatc agcagtatct
     8341 ctcattggaa ggcaaaccat cgctgactca gcggattgac tggtttattc aaagagtaca
     8401 ggaaaatcag aaaatgcagg aagaagaatc tgaaaaacaa gcatatatcg tagaagcact
     8461 gactgaggca aaagagcaga tcgaagactt gacctttaca ctgcaagaaa tgaaaggaac
     8521 ttcattatga caaatgattg gctgatccaa caattagatg aattaagcga aaaatcagaa
     8581 tatcaagacc gtgcgctctt atatgaaata aagaaactga tggctgagat agacgaacga
     8641 acaatgcagc tgcaaggaga aatcgacgga caatcttgga atcatctcaa ctggtgaaat
     8701 tttcttataa ataacaaaga taaaaatgtg cacagcgaca attatttgct acaataagaa
     8761 tatgaagcgt ttgataacgc tattatacat aaaaattggt ggtgaaaaaa atgtatgatg
     8821 aaaatgagtg gggagaaaat gttcggatga cccgaagaag acagcaaaaa caggcagctc
     8881 gtaagcggat gatcatgatt ggggcagcag ttgccgtgtt tttgatcata gcaggaggag
     8941 ttgcgggtct atattttcat ggaaaaaatt cgtcagaagc gaaaagtacg accataacgg
     9001 aaacaacgac aaagaaaaca gagacgaaac aaacaagtga gtcatcaact aaaacaaaag
     9061 agacacaaac ttctgaagaa aaaacagcaa atggtttgcc agagcaactt caagcaatcg
     9121 taaatgatta ttcgaaaaag atcgaagaaa agacgcctgc attgattgaa gaataccaga
     9181 cagaaattca gggaaatcaa gagggaatag ctggtttatc agcagttgct aaccaaaaag
     9241 cacgtgagct tcaagcaatc tccgacgaag ggatcagaaa attacgtgca gcgtatcagg
     9301 cagcagaaaa taaagacggt gtagacttag atactttgat caatcaatta tcggctaatt
     9361 atacgaatca tgtcgctaag atttccgata tttatttgca gactagcgcc tctcttcaag
     9421 cagagtcaac aagcacacaa gacacaacat cttctgattc tgagacagca gagtcggtag
     9481 aagcttctga aatgcaagct cgaacaacac aagacacaac agattcctct gaaactaata
     9541 ccaacagcgc gacttcacag gctgcgacaa ccgtagtttt ggaaggagaa ggtccaaatc
     9601 aaatagccga acgaactggc gtaccagtag aaacgatctt gtcgctgaat gggatgagca
     9661 tggagaatta tttcctgact cccggtgaga cgctaaaatt gaattgataa aagcaataaa
     9721 aaagaacgaa gcttccacta aggtgaagct tcgttctttt tttattttcc ggttcttttt
     9781 tcgcgtacaa agtaatggat cgtcatgtaa agcaagaaaa cgagtccaat aatcaatgag
     9841 attctcgttt caggattgat aaacatgaaa atcaatacga tagctaaaaa agcaagtgtc
     9901 acataattcg tataaggaga tagtggcatt ttaaaaggat gatcatgcat tttatctgcg
     9961 tgcttctttt tgaagcgaat ctcgctgatc aaaatcacta cccaaggaac cataccaggc
    10021 aaaatacttg agctgtatac gaaaacgaaa atattttctg actggttgat gaacattggt
    10081 agaagataat ttaagatcag accaattaag attccacctg agattgctac gacaggataa
    10141 aaaggcgcac cgtgacgaga tagtttcaga aattttttag acatctttcc atttatggca
    10201 agtgtataga ccatacgact tgcgctgaaa atccctgaat tgcaacctga taatgccgct
    10261 gtaatcacga caaaattaat cagtcctgca gcaaatgtaa tgccgacttt agaaaaagtt
    10321 tgtacaaaag gtgaaccaat ctcactcaat tgattccacg gatagatact cacaatgata
    10381 aaaatagcac caatataaaa aatcaagatt cgaccgatag tggactggac agcttcaatg
    10441 atcgtctttt taggattctc tgcttcacca gcagtcattc cgatcagttc gattccttga
    10501 taggaagcaa caacgatcga taatgcaaag aaaaatcctt tgacgcctcc agtgaagaat
    10561 ccaccatttt tccaaagatt tgaaatccct actgcctgac catgattacc aaatccaaat
    10621 aagattaccc caagacctgc aatgatcatc aaaatgatcg ttatgacttt gatgatcgaa
    10681 aaccaaaatt ctaattcgcc aaacgccttg acagaaataa gatttgccag cataagaaac
    10741 aagacgacaa tgattccggg aataattgcc ggcaaatcag gccaccaata attcatataa
    10801 gatcctacag caatcacttc acttattcct acgacaatat attgaaaaat attactccaa
    10861 gcggtcaagt agccagccac aggatggatg tactcgctcg cgtaatttgc aaaagaacca
    10921 gtcgaaggat caacgtaaag catttcgcct aaagcacgca taatcaaata caaaaagaat
    10981 cctgcaatcg cataggctcc catcactgag ggccctgtcc aacgtatggt tgaggaggca
    11041 cccataaaca gaccgacacc gatcgttcca cctagtgcga tcatttggat atgacgggca
    11101 ctaagacttc gagttaactt ttcttcttcc atacaaaaac tcctttcgtt gttgagtcaa
    11161 caagaaaaaa taatcaaacg tttcaacaca tgtttttcat tattaggtta gccttattga
    11221 actcttgatg tgattctaca gaaaattttc ataaaatgcg aggaaaattc gaaaaaaact
    11281 ttttcatcat tctacttgaa aatgctttcc attttagtta aactatattt aaataaaaag
    11341 ttaggtaaac ctaaaaagga gtgtaagatt tggaacgtac ggccattgta attcaagacc
    11401 tttcagccgc ttatcaaggg aaaactgttt tgaaaactat caacttgacg ataaatccac
    11461 aaaaaattac cggtattatc ggaccaaacg gtgcaggaaa atcaacattt atgaaatctt
    11521 tgctagagtt gataccagct actactggta aagtcacctt ttctggacag tcggtaaact
    11581 cagtccgtaa aaaaatagcc tacgtggaac agcgaagtga attggatctt tcctttccaa
    11641 ttgatgtatt aggcgttgta cttttaggaa catatccatc tttacgaatt ggacaaagac
    11701 ctgggaaaaa agaaaaagaa cgtgcaagac aagctttgaa aaaagtaggg atggaagaat
    11761 atgccaaaag acagatcagc gaactatcgg gtggacagct tcagagagtt tttattgcaa
    11821 gagctctagc ccaaggagca gaatggatct ttttagatga accattcgta gggattgatg
    11881 cgttaagtga acgaaagatc tttgacatct tgcaggaatt gaagaattca ggaaaaacga
    11941 ttttgatcgt ccatcatgat cttcataaag tagaagaata tttcgatgag gttattcttt
    12001 taaataaaca gctgatcgct tccggtccag tacaagaagt ctttacatcg aagaaccttc
    12061 aattggccta tggtgagatc atcagacatt tagtaaaagg aggagagaaa aaatgatcca
    12121 atcctttatt gatggattaa tgaattatca attcctgcaa tacgctttgg tcacttccat
    12181 gcttgtagga cttgcttctg gagtcatcgg ttcttttatt attttacgag ggatgtcttt
    12241 gatgggagac gctatctctc acgcagttct tccaggtgta gccatttctt atatgtttgg
    12301 ttttagttac atctttggag ccactgcttt tgggatgcta acagcagcag tgattggttt
    12361 cgtgactcag cggagccgat tgaaaaatga tacagcgatt ggaattgtct ttagctcctt
    12421 ttttgcatta gggattatct tgatttcata tgcccagagt gcgacagatt tgtatcatat
    12481 tttatttggg aatgtcttag ctgttaggga aagcgatttg ttgctgacag cactcgttag
    12541 tggaatcgtc ttgatatttg tctttttctt ttataaagaa ttgaagatca catcattcga
    12601 tccgacaatg gcaaaagctt atgggctgaa cacatcgctg atccattatc ttttgatgtt
    12661 ctttttgaca ttagtggctg tagtcagttt acagacagta ggaacaatct tggtgattgc
    12721 catgttgatc acaccagccg ccacggctta cttgctaacg aaccatttac tgaaaatgat
    12781 cattacagct gcaggaatcg gtatgctaag tgcagttgtc ggtgtgtttt tcagttatag
    12841 ttacaattgg ccatcaggag ctacgatcgt gttagcatgt accgcatttt ttatccttgc
    12901 ttttttattt tctccaacaa aaggaatttt atttcagaaa ggaagcagat agatgaaaac
    12961 aaaaaaatca ctctttttaa tcttggcagt tagtttcttg gtattagccg gttgcggaaa
    13021 gcaggcttct gaccaagcgg atgaaggaag taaagaaaag ttgtcagtcg tggctaccaa
    13081 ttcgatcttg gcggacatgg caaaagaggt cggaacagat caaatagata tccacagtat
    13141 cgtaccagtc ggaacagatc cgcatgaata tgaagtacta ccagaagaca tcaaaaaggc
    13201 aagcgatgca gatgttattt tatacaacgg tttgaatctt gaaacaggta acagctggtt
    13261 cgataacttg atggaaacgg ctaaaaaaga agaaggaaaa gattattttg cagttagcaa
    13321 aaatgtagaa cctctttatt taactagcgg tgaagaacat acaaaagcag atccccatgc
    13381 ttggctagac ctatctaacg gaataaaata tgtggaggaa atcgcacgta tattctctga
    13441 aaaagatgca gaaaatgcga cactctataa aaaaatgcag aagcatatgt ggaaaaacta
    13501 aaagaattag atacacaagc gaaggaaagt tttgcttcta tcgaagagaa caaaaaatta
    13561 ttagtaacaa gtgaaggggc attcaagtat ttttcacgag catatgattt gccagcagct
    13621 tatatatggg agatcaatac agaaagtcaa ggtacgcctg atcaaatgaa agcgatcatt
    13681 gatcagataa gagcaaaaga agtaccggtt ttattcgtgg aaaccagtgt agactcaaga
    13741 agcatggaac gggtagcaaa agaaacgggt ttgaaaatct atgataaact gttcactgat
    13801 tccatagcaa aagaaggaga acaaggagat tcttactatc agatgatgaa atggaatatt
    13861 gaaacgatcc atgaaggttt gtcacaaaca aaagaaagct gaaaagtgca aggtctagct
    13921 tgaggagcag accttgcatt ttttataaga gatatgaaaa aaaattatgc tgtttttcgt
    13981 cgtacagata ccttttttga agaaattccg ttatagttag agtaaggaag gaaggtgtgg
    14041 ctatgaattt tacacgtaaa gataaatata cgattaaaga agaagtcttt cgattgatca
    14101 gccagcagga aaataccgac aaggatcaag accttcaatt tcttgaagca gaattagcta
    14161 aattattcat agcaaatcaa aatgagacaa cagaaccaga tgaagagctg tacataggca
    14221 aatggttgtt tgatgaattt cagcctttag atgaaaatac cggtatcggt tattttgtgt
    14281 tcctttctcc agtcggcttc acacctggcg tattagttaa acaaaacgac gaaataaccg
    14341 tcaaagcatg gcctgcatta ttttcagtca tcaaagcaga aggagtaatc aaaaagatcc
    14401 agtcggccta ttataaaagt caatttattg ccaacagcaa attaatgaat atgttttata
    14461 atcgtctagt agaaattgct aggcctggga aaatcccgca aacattcacg tacacacagc
    14521 aaaaaaatct ctatgaagaa gtattgtatt cccgcgatct gtctgagttt tcgccatggg
    14581 aagaggtaag gctccatgaa ctgttaagta caaaaccagt ttcattgaat ttttttaatg
    14641 cattagataa caaaagaaaa ttaatttcaa caaattattg ggaagtattt gctgcaagaa
    14701 aaaaatggat ggaagaaaag aagattgccg atcagcagat ccagtttgtc cagctgaaaa
    14761 aagaaaaact atattatggg ttatacagcc gttaacagca agcgaggaag accatgacat
    14821 taaaagaaga aatcatacaa gaaagcaaac gattaggaat tgacaaaatc gggttcacca
    14881 cggcggaacc ttttgattca ttaaaagaat cgttggaaga acaaaaagca gctggccata
    14941 cttctggctt tgaacatcca aatatcgatg agcgtcttta tccagaaatg acatttgatc
    15001 agccgaaatc gatcatttcc attgcacttg cgtatcctac taggatccat gaagaagtgc
    15061 ctagggatga aaaacgcggg cagtttgctc gtgcttcttg ggggatcgat taccatgata
    15121 tactcagaga ccgtttgaac cgattgatcg aattcatcca aacaagagca gaaaaatggc
    15181 aaaaagaaga ggaatggcgt ctagctccgc aagtagatac tggagaactg atcgatgtcg
    15241 ctgtagcaca gcgagctggt ttaggattta ttggacgaaa cggattgttg atcactgaag
    15301 agttcggttc atttgtctat cttggtgaga tcgttactaa tattgtattt gagcctgata
    15361 aaccaggaga atttggttgc ggtgactgta tgcgctgcgt gactgcttgt ccgacaaaag
    15421 cattgcttgg tgacggaaga atgaatgccc agcgatgttt gtcttatcaa actcagacaa
    15481 aagggatgat gccggaagaa tatcgtaaaa aaatgcgaaa tgtcatttat ggatgtgata
    15541 tctgccagtt agtctgccct tacaatcaag gaaaagattt tcattttcat gaagagatgg
    15601 aaccaaaagt agacgaaata tatccaaaat taaagccaat gctttcgatg tctaataaag
    15661 acttcaaaca gcaatttggg catttagctg gttcatggcg tgggaagaaa cctttgcaga
    15721 gaaacgcatt gattgcttta gctaatctag gtgatcgaac ggcattgcct gagatttcag
    15781 aatgtttaac agatgtccgc cctgtcatca gaggaacagc agcttgggct attggacgtc
    15841 taggaacaaa agaaccggaa aaatggcttg atgtattaga tgaaatatta gctaaagaaa
    15901 cagaagaagt cgttataaac gaattggaac atgcccagaa attattgcgg agaaagttaa
    15961 aataaaaaaa tagttaacat tattcaaagt tt
//
LOCUS       DAZUKA010000066        14961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000066 DAZUKA010000000
VERSION     DAZUKA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.066"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACG8PN_001644"
     CDS             5..2377
                     /locus_tag="ACG8PN_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4861788.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACG8PN_001645"
     CDS             2438..3898
                     /locus_tag="ACG8PN_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4861789.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACG8PN_001646"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACG8PN_001646"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4861790.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACG8PN_001647"
     CDS             6129..6278
                     /locus_tag="ACG8PN_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861791.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACG8PN_001648"
     CDS             6353..6946
                     /locus_tag="ACG8PN_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4861792.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACG8PN_001649"
     CDS             6959..7858
                     /locus_tag="ACG8PN_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4861793.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACG8PN_001650"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACG8PN_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4861794.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACG8PN_001651"
     CDS             8724..8984
                     /locus_tag="ACG8PN_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861795.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACG8PN_001652"
     CDS             9429..9635
                     /locus_tag="ACG8PN_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4861796.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACG8PN_001653"
     CDS             9656..9886
                     /locus_tag="ACG8PN_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861797.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACG8PN_001654"
     CDS             9902..10339
                     /locus_tag="ACG8PN_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861798.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACG8PN_001655"
     CDS             10332..11039
                     /locus_tag="ACG8PN_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861799.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACG8PN_001656"
     CDS             11203..11403
                     /locus_tag="ACG8PN_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861800.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACG8PN_001657"
     CDS             11419..11598
                     /locus_tag="ACG8PN_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861801.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACG8PN_001658"
     CDS             11923..12186
                     /locus_tag="ACG8PN_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS4861802.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACG8PN_001659"
     CDS             12180..12530
                     /locus_tag="ACG8PN_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4861803.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACG8PN_001660"
     CDS             complement(12554..13168)
                     /locus_tag="ACG8PN_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4861804.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACG8PN_001661"
     CDS             13482..13904
                     /locus_tag="ACG8PN_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861805.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACG8PN_001662"
     CDS             13914..14117
                     /locus_tag="ACG8PN_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861806.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACG8PN_001663"
     CDS             14328..14912
                     /locus_tag="ACG8PN_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4861807.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5512 a         2429 c         3035 g         3985 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg a
//
LOCUS       DAZUKA010000067        14668 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000067 DAZUKA010000000
VERSION     DAZUKA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.067"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1792
                     /gene="yfcC"
                     /locus_tag="ACG8PN_001664"
     CDS             53..1792
                     /gene="yfcC"
                     /locus_tag="ACG8PN_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4861808.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1949..3133
                     /gene="iadA"
                     /locus_tag="ACG8PN_001665"
     CDS             1949..3133
                     /gene="iadA"
                     /locus_tag="ACG8PN_001665"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4861809.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3244..3447
                     /locus_tag="ACG8PN_001666"
     CDS             3244..3447
                     /locus_tag="ACG8PN_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4861810.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3548..4210)
                     /locus_tag="ACG8PN_001667"
     CDS             complement(3548..4210)
                     /locus_tag="ACG8PN_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861811.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4223..5725)
                     /locus_tag="ACG8PN_001668"
     CDS             complement(4223..5725)
                     /locus_tag="ACG8PN_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861812.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6273..7952
                     /locus_tag="ACG8PN_001669"
     CDS             6273..7952
                     /locus_tag="ACG8PN_001669"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4861813.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8079..8558
                     /locus_tag="ACG8PN_001670"
     CDS             8079..8558
                     /locus_tag="ACG8PN_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4861814.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8777..9457
                     /locus_tag="ACG8PN_001671"
     CDS             8777..9457
                     /locus_tag="ACG8PN_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4861815.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9465..10796
                     /locus_tag="ACG8PN_001672"
     CDS             9465..10796
                     /locus_tag="ACG8PN_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4861816.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10811..11773
                     /locus_tag="ACG8PN_001673"
     CDS             10811..11773
                     /locus_tag="ACG8PN_001673"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4861817.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11884..12222
                     /locus_tag="ACG8PN_001674"
     CDS             11884..12222
                     /locus_tag="ACG8PN_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4861818.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12301..13008
                     /locus_tag="ACG8PN_001675"
     CDS             12301..13008
                     /locus_tag="ACG8PN_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4861819.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13111..14592)
                     /locus_tag="ACG8PN_001676"
     CDS             complement(13111..14592)
                     /locus_tag="ACG8PN_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861820.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4656 a         2532 c         3155 g         4325 t
ORIGIN      
        1 tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa atttgaagaa
       61 aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac cccatacgta
      121 tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg ttccagcagg
      181 aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac
      241 tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt atgtgtttga
      301 tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg ttccggaaaa
      361 aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa aactagatga
      421 aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct atgatacttc
      481 taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg gctttggttt
      541 cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg ctgtagggat
      601 cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta ccggagcgat
      661 cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt tagctttacc
      721 gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag aagaggtaat
      781 tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact cgatcgtagc
      841 agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga tgagcccgtt
      901 tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg cgacattccg
      961 attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga tatatgcaga
     1021 aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt atttccgttc
     1081 tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata aattgatctt
     1141 attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa aaggctatta
     1201 cattccagaa atcgcttctc aattctttgt gatgggatta gttgctggaa tcattgctgt
     1261 tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc
     1321 agctgattta gctggtacag caatcgtagt aggtatggcc aaagggattc ttcttgtctt
     1381 gggaggatct gatgcatctg ttccttcagc tttgaatacg attttgcatg ggatcgggac
     1441 ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg atgtacatct tccaaagttt
     1501 gttcaacttg gttgttacat caaattcagg acaagcagct ttgactatgc caatcatggc
     1561 tccactggca gatttagtag gtgtatccag acaagtagca gttcttgctt atcagcttgg
     1621 ttcaggtttt atggacgctt ttacacccgt atcagccagc ttgattggtg tgctaggtgt
     1681 tgcacggatc gaatgggcaa aatgggcaag attccaaatc aagatgcaag gattcctgtt
     1741 tgttttagga actattttca tcatgattgc cattgcaata ggtctgcagt agtactggag
     1801 acctgtaaat aaaaaagtaa tccaaacagt agataaggac tccaagaacc gtcattttac
     1861 ggtaaaatca aaggagtaaa gatcctatcg tttggattat tttttaagat cctatcatca
     1921 gttatactta aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc
     1981 accagattac ttaggaaaaa tggatgtact gattgccggt gggaagattt tggctgtaga
     2041 agaacagcta gaaggcggct acgaaggagt ggaagtagaa gaactttcag gtgagggaaa
     2101 agtactaacc ccgggtttca ttgactgtca tttccatatt ttaggtggag gaggagaagg
     2161 cggttatcaa aatcgaacgc cagaagtcac attgagccaa ttgactacag ctggtgtaac
     2221 gacagttgtc ggttgtttgg gaacagacgg agaaggtaga gatatgactg cgttgatcag
     2281 caaagcgaaa ggtctggaag cagaaggaat ctccacttat gtatacgaag gttcgtaccg
     2341 attgccggta aaaacagtaa cagattctat catcaaagac tttttgacaa ttgataaaat
     2401 tatcggaatc ggagaaatcg cagtatccga tcaccgttct tctcagccaa gttttgaaga
     2461 gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg ttatccggaa aagctgggat
     2521 catcaatgtc cacttaggcg gaggaaaaag aaagctagag ctattgacac gaatcgtaga
     2581 agaaacagaa attccgatca cacaattttt gcctactcat gcaaatcgca caccagaatt
     2641 atttgaagct tgcgtggcat ttgccaaacg tggtggcacg attgacttca ctgctagcga
     2701 agatcctgat ttttgggaga agacagatgg tgaagttcgc ttcagcaaag cactgaaacg
     2761 attgatcgag gaagatgttt ctttagataa tttcacgatg acctcagatg gacaaggaag
     2821 cttgccttat tttgatgaaa acaatcattt cctagggtta ggagtaggaa gtgccaaggc
     2881 attgctagta gggatcaaag aagctgtgca aaaagaaagt atccctttgg aaatcgcctt
     2941 acgagctata accagtaatc cagcacgtat attgaaattg gataaaaaag gaaaaatcga
     3001 aattggtgct gatgcagatc tgtgtatctt ggataaagaa acgcttgata ttgatacagt
     3061 tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc aaagtatggg ggactttcga
     3121 aaaaagtttt taatcaacag ctctaactat cttttttaga aaacgagcgt tctgtttgtc
     3181 tgtatctgtt gttgatgtta ccctgaaggt agatcaataa acaaactaag aggaggaagc
     3241 acaatggcag atttaaaagg acgttctaaa gatatgaaag ataaagtagt cggtgaagga
     3301 aaagaagcat acggcaaagc aacagacgac aagagcaaag aaacagaagg gaaagcacaa
     3361 tcactaggtg ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt caaagacaag
     3421 ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta ctaattgaat caagcaaata
     3481 aaaaatccga accatatctg cgagacagat atggttcgga ttttttattt gatcattatc
     3541 ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag acttcatcac
     3601 agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt tgttcatggg
     3661 cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga tttccagtag
     3721 gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata gcgactcgct
     3781 gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca tcaattccta
     3841 cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc ttagtgattt
     3901 ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg taagcttgga
     3961 aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc agtgaacgat
     4021 cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc gaaagaaaag
     4081 tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct tcgaaattca
     4141 gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc aattgttctg
     4201 tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt aatcttaata
     4261 tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact ccggctaata
     4321 gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca gaaacagcaa
     4381 atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct tgtctgttac
     4441 ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct gtctgttggt
     4501 caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa gcaactccta
     4561 atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt ttacttctgg
     4621 attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg gtaatcataa
     4681 tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca tttttagaaa
     4741 aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca tttgattgta
     4801 agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca tccattttat
     4861 ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat gtcccattta
     4921 gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg aagttgaatt
     4981 tcatacccat actgccgctt gtttcgctgg attcataaat tcctttgatc gtcatttcat
     5041 aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact gccaggtcat
     5101 ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta
     5161 ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt tgtgcagttg
     5221 ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg cccattcctc
     5281 cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa tcagaactag
     5341 tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag gaataactct
     5401 ttacattatc taattcagct attttttccg cgtccgttaa ggaaactggt gtaagagaaa
     5461 aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt
     5521 ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact tctttttgtg
     5581 cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc acaaaaataa
     5641 gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact tttgtacttt
     5701 tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc atttgataat
     5761 agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat caacatgagg
     5821 ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt
     5881 atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta
     5941 cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg atttagttta
     6001 tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg ctatagcgtt
     6061 tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca tccaactgtc
     6121 agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga aatagtgatt
     6181 ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat cgacatcttt
     6241 aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt atcccattag
     6301 gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag gatattttta
     6361 ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc
     6421 ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg
     6481 gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct gtaccagtat
     6541 tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat gaagcagcca
     6601 aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt ggtgaaacag
     6661 tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc aatctgaaaa
     6721 caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg gctatcccaa
     6781 tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc ttagccttac
     6841 taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc caaatagcag
     6901 atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct tgtgtagcaa
     6961 gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa cgaaaagttg
     7021 tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc
     7081 aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat gatccagaag
     7141 aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg caaaaaatgg
     7201 caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat atcactacta
     7261 cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt tatcgagctg
     7321 gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc aatcctaatg
     7381 acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt caaggagagt
     7441 atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca tacaaaaata
     7501 tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg agtgttgcag
     7561 gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat atcggaaaca
     7621 ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg gttgtcacta
     7681 tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat tacttcacgg ggattcgttt
     7741 acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg
     7801 agaaacattt agaaagcaat gattttgagt ggagcaaatt aaagcaagac atccgagatc
     7861 aattaggccg ttacttattt gaacaaacaa aacgccgccc agtgatcttg ccagtcatta
     7921 tggaagctac tcaacgtcga aataaaaaat aaacaaaaag agtaagatga acctctctat
     7981 agagtgctcg tcttgctctt tttttgcgaa aaacaaataa tttttgaata cagcatccct
     8041 cgctttataa taaacgatag aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg
     8101 aacgttattt agaagaacaa aagatcagct atcaaccact tgattttagt catgtggtag
     8161 aagaatcttt cacagaagaa ctacaaaaaa gagggattga tccagctttg atatgtaaaa
     8221 ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc attgattcct ttgaacgatc
     8281 acttagatta caaaaaaacg agacaagtga cgaaagatcg taaagttggt tttcctggaa
     8341 tggattttgt attaatgcat acaggatatc cccacggagc aaacacccct attggaataa
     8401 aactcgcaca tcctgattat tgttttttag cagacagttc tttaaaagaa aaagaagaag
     8461 taattgtgtc aagcggtgaa atcgggagaa gccttcggat aaaagtagcc gatttagaaa
     8521 agatcgtcca tcctatttac gctgatttaa ttagttaagg gacgagaaga gaagctaagc
     8581 agagcagaga cctcaaactt gagattatat ctgcttggta cttggtgcat cctgtattat
     8641 ctgttacaat tgctttgtac atactgtact atcaatccaa tgaaaatata tttagcagct
     8701 atattttttc attgatgtgc aagtgatgaa atcgttttta ataaaaaaga tacagagaaa
     8761 acgcgaggtg taaaatatga aaattacgta tcatggacat tcctgtttaa caattgaaat
     8821 ggaaaatggc caacgcgtga tgattgaccc gtttattaca ggaaatccat tgacagactt
     8881 aactgcagaa gaagtaaaga cagattggtt gctgattact catggtcata gtgaccacat
     8941 aggagatatg attcctattg caaaagaaaa tcaagccaca attatcagta ttgttgagat
     9001 tgctgattat gcgaacagca gaggagtaga ttcattcggt atgaatatcg gtggaaaaca
     9061 tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag catagttcaa gctatgaagt
     9121 agacggtatc gttcagtata tgggcgagcc tagcggaatt attattcaag cagaaggaaa
     9181 aacgatttat catgcagggg atactgctta tttcagtgat ctaggattgc ttgcagaaga
     9241 attcgatatt gatgtcgctt tcttgccgat tggcgataac tatacgatgg ggccagaaga
     9301 tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta gttccaatcc attacaatac
     9361 tttccctgtc attgaacaag atcccgaatc ttttgtagag ttactggaag atagtgaagg
     9421 aaaaataatg agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg acaaaacatg
     9481 atttgatttt agaatatatt gaaagtttac ctgttggcaa tcgaatttct gtaagaagca
     9541 tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa
     9601 atgtcggact agtttcaaca attcaacgag tagggaccat ccgtatcgaa cgcaaattaa
     9661 aaaaacatat tgaacgtttg acatttggcg aggtcgtcag gatcattgaa ggggacgttt
     9721 taggcggggc ggcaggatta gacaaggtct tgaataaatt cgtcatcggc gcgatgacta
     9781 aacatgccat ggaacgctat attacgccag gatcactaat gatcgtcggc aaccgggcgg
     9841 aagtccaaaa attagcattg gaagatggcg ccgcagtatt gatcacagga ggattcaata
     9901 cgactgctga gattgcaaag ctagctgatg atcttgaaat gccgatctta cgcacctctt
     9961 atgatacatt tacagtcgca accatgatca accgagcgtt aagcgaccaa ttgatcaaaa
    10021 aagatatcat gcttgtcagt gatatctata tgccagctga aaagactcat tatttgcagc
    10081 agacagatac gatttctgat tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc
    10141 cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac ggcaaaagat gttttaggga
    10201 aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa
    10261 caatgagtat tgcctctgtt agccatcaaa tgatctggga tgggctagaa gtcatgccgg
    10321 tcgtttccga tgacttgaca ttggaaggct tgattacccg gcaagatgta atgaaagcga
    10381 tgcagcttgt ccagcgacaa ccgcagattg cagatacgat ttcggatcag atatctggaa
    10441 atatccatac gattgatact gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat
    10501 tcgtagtagc accacaaatg gtcaatggcg ttggaaccat ttcttttggc gtacttagcg
    10561 aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga
    10621 ttgaacaagt gaacctacat tatttgcggc tgatccaatt ggaaagcgaa ttagacattc
    10681 gtccacgtgt attggaaatc ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg
    10741 aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag
    10801 caggagtgaa atgacagtac agcaagaaat agtagataca ataaaaaaat acgataaaat
    10861 cattatccac aggcatttgc gccctgatcc tgatgcatta ggatcacaag taggattagc
    10921 cgaactatta cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg aaacgattga
    10981 agggcttcgc tttttagcag aaatgcaaga agttgaagat gtcttctacc aaggagcgct
    11041 agtcatcgtg actgatacag ctaattcacc cagaatcagt gatcaaagat atcagttggc
    11101 agaaaaactg atcaaaatcg accatcatcc caatgatgac ccttatggag atcttgtgtg
    11161 ggtaaataca aaagcaagta gctgtagtga gatgattgca gaatttgctt tgatgtttcc
    11221 tgaatcttta aagatgaacg ttaaggctgc acggttactt tatgcaggaa tcgtcggaga
    11281 tacagggcgc tttttgtatc cctccacgac gtcccgtaca ttagagattg cttcgatatt
    11341 gagaaattat ccatttgatg caagtgcttt gaacagagag attgaacaaa tgccgatgaa
    11401 agtcgctaaa ctttccggtt atctttatca aaatatccag gtggatgaaa atggtgcagg
    11461 aagaataatc cttccgcaag aaattcttga taaatatgga atcgatgatt cagagacagc
    11521 ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg gcttggggga tttttgttca
    11581 gcagccttca gggtattacc gcgtacgttt acgttctaag gggccgatca tcaatgaatt
    11641 ggctaaacgc catcatggag gaggccatcc attagcaagt ggtgcgaatg caaaagattt
    11701 agcagaagta gaggaaatct ataaagagat ccaagatata tgccaatcgt ataagagcag
    11761 aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat
    11821 cttttcaaga tcttcttttt ccgttacaat agtgctcact aaagatttta aaggagaaag
    11881 aagatgacat taccaaattg cccagaatgt ggctcagagt acacatatga agatcgaggc
    11941 ttgttgattt gcccagaatg cgggaacgaa tggaacaaag aagaacaaga aactgtcata
    12001 gaagacggat tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg
    12061 acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa
    12121 gtcaagaaca tccgtttagt agaaggcgat cataatatcg attgcaaggt agaaggattt
    12181 ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt
    12241 tcacattctt ttcctactac taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa
    12301 atgtatggtt atggaatgta tggtttttta gatccgacat ttcttttggt cattcttgga
    12361 ttagttattt ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa
    12421 gtgagaagca aaaacaatgt gactggtaca caagcagcac aatatatatt acaaagtcaa
    12481 cagataaata atgtcggtgt tcaacaaatt gccggagatc tcacagataa ctacaattca
    12541 ggtaataaga tgttgagttt gtctcaagca actgcacagt ctacttcagt tgctgcgata
    12601 ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg cttcgggtta tgtcccattg
    12661 aagattcgtg cagcacttgt tccagcagct aactttggtt cgatgatttc gttccccttg
    12721 attatggtag gtgtattgct tagctggaac tctacgttaa tcaatatcgg gattttcgct
    12781 ttttctttag cattactttt ccaattggtg acattaccag tcgaatttaa cgcgtctcgg
    12841 cgagcaatcc aaattttgag tgaaggcgga ctactgacgg aagaagaagt accgatggca
    12901 agacacgtat tatttgcagc agcactgact tatgttgcag cagcgctttc gacattcctg
    12961 cagctgttgc gcttgatctt attgttcggc ggcaaccgaa gagattaaaa taaagaagtt
    13021 gtgaaatcag cgctttgatc tgagctaata ccgagagtta gctgattgaa taccatttat
    13081 ttggaataag aggatgtgac aagaattttg tcacatcctc tttgttttgt tctcataacc
    13141 ttttttcata tacagttcat gaaccgcttt tattaacttg ccaaataaca ctgtattgtg
    13201 cgttgtatcc agatagaaag tctccgtttc atctgaaatt ttctcatgct gatctgtaat
    13261 gatcaaatca gcatgttctg aatatgcggt aattttaaca gcatctttgc gaaataactg
    13321 ctgcaaatgg ctttgaagat gataatttcc tgtaaactgt ttggttatct gcaaatggat
    13381 gagtaactgc tctttcgttt ctgtttgtag taagtgttgg atcaattgac taaggtattg
    13441 actgatttgg tttctttccc cattttttga aaaaattgta tcgatttttt ctcttaattt
    13501 cttttcctgt ataaaagaac tagaatgatt ggacgtggct tctggaaagc aaagtgtaga
    13561 aaaactactg ctggattcac ccaacagttc gaaaaaaaca actgccaaca caagaaaata
    13621 aaaataaatt ggtattttat tttctaaatg gttgagagga aactgttgag ttaagtaagt
    13681 catcaattga ccgacttgtc gacaaataag gtttttgctt gtggaaaata aaaaaccaat
    13741 ctcctgtttt tgtcgaaaag agatttgatt tggaaaaaaa caatagaaaa aataattaaa
    13801 gtaggctctc tctgtttctg tctcttcaca agggcgactc actgaaaaag aagtaaagta
    13861 ttctgaataa tcatgtgtat gctgcaataa gtccatcaat tcagtgattt cagaagaagt
    13921 ttttggaaaa tatgcctgtt gtttgtttcg taatatgaat aaagttaaca gcaagtagat
    13981 atttttttct tgttttgaaa ttttttctat ttgctcatct gtactgttca tttttagtgc
    14041 atttaattca agtgaggaaa atggccaatc gttacctcca aatgtatctg aaagaaaaaa
    14101 gtaaagatag agacggatag ataactcatt tccttcaaga gtacagcaat tttttacaga
    14161 taggatccgt aaatggaatt ttattaagga atgattgagc tgttttgtca gtttttcgag
    14221 ataggagggc gatatataaa gttgttcaag taaatatttt tttggtacag cagtttgtgg
    14281 ataattgatt agtaacatca aatagttaaa acgattggat tcttttaggt agcataattt
    14341 taacttagaa aaaacagcta actttttagt ttgatcggtt tctttggttt ggatctctcc
    14401 tttatgatta gaataaataa attcatcaat caataaaaat tgcttgagtg atacatttat
    14461 ttttcggatc agttctttga ttgtccgttt tggaatatgt aagtcattgg ataattcttg
    14521 taaagaacaa ttattttttt gaatgaccat ttgtagaaca agtaactctt tttttcttgc
    14581 ttcaattagc ataaatacct cctttttatt ctgtttttgt atatagttag acaaaaactg
    14641 tgagtggaaa gtcatttcag tctgtgtt
//
LOCUS       DAZUKA010000068        14623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000068 DAZUKA010000000
VERSION     DAZUKA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.068"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..771)
                     /locus_tag="ACG8PN_001677"
     CDS             complement(205..771)
                     /locus_tag="ACG8PN_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4861821.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(771..1727)
                     /locus_tag="ACG8PN_001678"
     CDS             complement(771..1727)
                     /locus_tag="ACG8PN_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4861822.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1727..2383)
                     /locus_tag="ACG8PN_001679"
     CDS             complement(1727..2383)
                     /locus_tag="ACG8PN_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4861823.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2753..4771
                     /locus_tag="ACG8PN_001680"
     CDS             2753..4771
                     /locus_tag="ACG8PN_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4861824.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4841..5047
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5198..7612
                     /gene="leuS"
                     /locus_tag="ACG8PN_001681"
     CDS             5198..7612
                     /gene="leuS"
                     /locus_tag="ACG8PN_001681"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4861825.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7960..9513
                     /locus_tag="ACG8PN_001682"
     CDS             7960..9513
                     /locus_tag="ACG8PN_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4861826.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9894..11678)
                     /locus_tag="ACG8PN_001683"
     CDS             complement(9894..11678)
                     /locus_tag="ACG8PN_001683"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861827.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11693..13413)
                     /locus_tag="ACG8PN_001684"
                     /pseudo
     CDS             complement(11693..13413)
                     /locus_tag="ACG8PN_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13513..14274
                     /locus_tag="ACG8PN_001685"
     CDS             13513..14274
                     /locus_tag="ACG8PN_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4861828.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4750 a         2702 c         2916 g         4255 t
ORIGIN      
        1 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
       61 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      121 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      181 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      241 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      301 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      361 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      421 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      481 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      541 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      601 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
      661 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
      721 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
      781 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
      841 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
      901 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
      961 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1021 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1081 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1141 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1201 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1261 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1321 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1381 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1441 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1501 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1561 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1621 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     1681 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     1741 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     1801 gccaagaaag actgagtaag taaccgccga tgatatcagt aggaaaatga acgcccagat
     1861 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     1921 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     1981 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2041 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2101 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2161 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2221 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2281 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2341 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2401 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2461 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2521 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2581 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     2641 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     2701 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     2761 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     2821 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     2881 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     2941 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     3001 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3061 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3121 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3181 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3241 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3301 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3361 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3421 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3481 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3541 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3601 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     3661 aaatggctct tcgacgaata attccggtaa caataccaat tcttcaggaa atgctggtac
     3721 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     3781 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     3841 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     3901 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     3961 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     4021 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4081 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4141 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4201 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4261 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4321 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4381 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4441 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4501 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4561 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4621 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     4681 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     4741 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     4801 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     4861 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     4921 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     4981 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     5041 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5101 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5161 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5221 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5281 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5341 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5401 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5461 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5521 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5581 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     5641 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     5701 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     5761 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     5821 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     5881 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     5941 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     6001 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6061 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6121 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6181 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6241 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6301 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6361 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6421 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6481 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6541 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6601 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     6661 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     6721 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     6781 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     6841 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     6901 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     6961 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     7021 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7081 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7141 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7201 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7261 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7321 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7381 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7441 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7501 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7561 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7621 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     7681 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     7741 tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg
     7801 taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat
     7861 tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt
     7921 tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt
     7981 atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga
     8041 ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta
     8101 tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta
     8161 aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt
     8221 tttcttctac ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt
     8281 agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt
     8341 tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta
     8401 ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga
     8461 atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct
     8521 tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt
     8581 cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata
     8641 aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat
     8701 gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat
     8761 ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg
     8821 gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg
     8881 ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg
     8941 tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca
     9001 tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt
     9061 tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg
     9121 tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc
     9181 aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt
     9241 gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt
     9301 ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa
     9361 ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca
     9421 gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt
     9481 cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc
     9541 agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta
     9601 actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta
     9661 ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa
     9721 aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa
     9781 aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag
     9841 ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct
     9901 atctgaaatt gactattata aagactggca taaagcgttg gtgattggag tagttcagaa
     9961 tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca
    10021 atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt
    10081 gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata
    10141 acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga
    10201 gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata
    10261 aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt
    10321 ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc
    10381 atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat
    10441 tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt
    10501 cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact
    10561 tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt
    10621 cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct
    10681 aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa
    10741 gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag
    10801 ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag
    10861 gcgagttgat taatgaaacg aatggccgga tagatagcac agttcataaa ttgagcacgt
    10921 ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc
    10981 tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa
    11041 acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt
    11101 gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata
    11161 taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata
    11221 cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca
    11281 cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct
    11341 tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat
    11401 gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct
    11461 tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga
    11521 tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa
    11581 ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt
    11641 atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc
    11701 cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac
    11761 cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac
    11821 tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc
    11881 ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat acataatctc
    11941 tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc ctgaataatt
    12001 agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca ctagttcttc
    12061 taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt catttcccat
    12121 ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga cataactgat
    12181 gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa gggttacttt
    12241 tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt ttccagatcc
    12301 ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag
    12361 aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt
    12421 ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt cgatctctgc
    12481 ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa tgaccaatac
    12541 catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta cagcagtcac
    12601 gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca aaatagaagc
    12661 ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata ttcggttcac
    12721 tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt cttcatatga
    12781 ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca atgtgatggt
    12841 gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt tcatcagtcg
    12901 ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta gtaatagaga
    12961 ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta agatcatttg
    13021 gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc ttgtcaaaag
    13081 agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt tacggaaata
    13141 tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt aactagaggc
    13201 aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa ttttttttat
    13261 aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta attttggtac
    13321 acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag cgagtccttt
    13381 gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt cctgatactt
    13441 ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa actgaaaaag
    13501 aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact atttgatttg
    13561 ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag tagacaagaa
    13621 gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga tatcgttttt
    13681 tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa aacattgacg
    13741 gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact ggtggtgatg
    13801 aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat gatcgataca
    13861 aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc aacggagttt
    13921 gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg gatgatgagt
    13981 atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag tgaagtacat
    14041 ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg tcgattctta
    14101 ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag agaaagaatg
    14161 tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac gaatacggat
    14221 gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa ataggaaata
    14281 tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa ttttctggtt
    14341 cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt tagtttttta
    14401 gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat acgatgtaat
    14461 tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata attcatggtg
    14521 aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta tcagatgaga
    14581 atgataaatt aaataggtgc ataaaagcat tacacttaaa ata
//
LOCUS       DAZUKA010000069        14565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000069 DAZUKA010000000
VERSION     DAZUKA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.069"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..298)
                     /gene="ssrS"
                     /locus_tag="ACG8PN_001686"
     ncRNA           complement(104..298)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8PN_001686"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(370..1656)
                     /locus_tag="ACG8PN_001687"
     CDS             complement(370..1656)
                     /locus_tag="ACG8PN_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4861829.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1794..2465
                     /locus_tag="ACG8PN_001688"
     CDS             1794..2465
                     /locus_tag="ACG8PN_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4861830.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2431..2919
                     /locus_tag="ACG8PN_001689"
     CDS             2431..2919
                     /locus_tag="ACG8PN_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4861831.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2932..3879
                     /gene="prmA"
                     /locus_tag="ACG8PN_001690"
     CDS             2932..3879
                     /gene="prmA"
                     /locus_tag="ACG8PN_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4861832.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3880..4644
                     /locus_tag="ACG8PN_001691"
     CDS             3880..4644
                     /locus_tag="ACG8PN_001691"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4861833.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4877..7090
                     /locus_tag="ACG8PN_001692"
     CDS             4877..7090
                     /locus_tag="ACG8PN_001692"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4861834.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7101..7547
                     /gene="dtd"
                     /locus_tag="ACG8PN_001693"
     CDS             7101..7547
                     /gene="dtd"
                     /locus_tag="ACG8PN_001693"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4861835.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7718..8977
                     /locus_tag="ACG8PN_001694"
     CDS             7718..8977
                     /locus_tag="ACG8PN_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4861836.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9066..10013)
                     /locus_tag="ACG8PN_001695"
     CDS             complement(9066..10013)
                     /locus_tag="ACG8PN_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4861837.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10407..11699
                     /gene="hisS"
                     /locus_tag="ACG8PN_001696"
     CDS             10407..11699
                     /gene="hisS"
                     /locus_tag="ACG8PN_001696"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4861838.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11714..13483
                     /gene="aspS"
                     /locus_tag="ACG8PN_001697"
     CDS             11714..13483
                     /gene="aspS"
                     /locus_tag="ACG8PN_001697"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4861839.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13592..14467
                     /locus_tag="ACG8PN_001698"
     CDS             13592..14467
                     /locus_tag="ACG8PN_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4861840.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         4775 a         2635 c         3165 g         3990 t
ORIGIN      
        1 gcgataatca ttaaaatcct aatcatgaac aaatcctcca aaataagcaa caaatagaaa
       61 aagcaatttg ccaagagcaa attgctttat tctttaaaag atggtaatct ccgttaatgc
      121 cgttgtttgt cctgtagtat tgatcccgcg aattcaagca ggtgggtccc atggtcgtag
      181 cttcgaacta ccaaatgata aggcatgtta ccagcagtga cacaagacgg gtcccaagat
      241 atcaataaaa atgttcggtc aacacacaaa gaggacaatt cgaacatcac agatttccca
      301 cctctatagt agcatatgaa tttaaaaaga gcaatgagag agctcgcaga aacagaaccg
      361 ttttcttgtc tagttttttg gaggatgctt tttccactct tttatacggt aatactgttg
      421 acccagagcc tgttcatatt tccctgtatc ttttggctga taatactgag cattcttcag
      481 tttatctggt aaatattgct gattgaccca agcctgatca aaatgatgag gatactgata
      541 acccacgcca cggttgagtg atttcgcacc tttgtaatgg ctatctctca aatgatcagg
      601 aacatctcca gctttgccct ctcgaatatc agcaagtgct gcatctaatg ccatgtaggc
      661 agaattggat tttggcgaca agcaaagatc gacgacgacg tctgctagtg ggatacgtgc
      721 ctcaggcaat cccaattttt cagcagctaa tacagcgttc actgtacgag ccgcagcagc
      781 aggattacct aaacctatat cttcataacc gataaccatc agacgccggc aaatacttgc
      841 cagatcacct gcctcaacta aacgggcgag ataatgaagt gcagcatcca catcacttcc
      901 acgaatcgat ttttgaaaag ccgaaataac gtcataatga gcatctccat ttttgtcatg
      961 ggtaagagct tttctttgga cacattcttc aatgatcgac agtgtaagat gaatccttcc
     1021 ttctttatct gaaggagtcg atagtgtagc cagttccagc ccattcaatg cactccgcaa
     1081 atcgccgttc gttgcacgcg aaaggtgaag aagagcatct tcgtctaact ggattgcttg
     1141 ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg actgccaact ggatgtcttg
     1201 ttctgttaat ggtttgactt caaagatctg tgtacggctg cggatcgctg gattgatggt
     1261 aatataagga ttctcagtag tcgctccgat caagatgatt cggccacttt ctaaatgagg
     1321 aagcagaaaa tcctgttttg ttttatctaa tcggtgtact tcatccaata agaggatcac
     1381 tgtaccactc atttttgctt cttccgctac aacttgtaga tccttttttg aatctgtagc
     1441 ggcgtttagc atgcgaaatg cataattagt agagccggcg atcgcacttg cgatactcgt
     1501 ttttccagta ccaggaggtc catatagaat catggaagag agcatgcgag catcgaccat
     1561 gcggcgtatg atttttcctt ccccgactag atgctgctga ccaacgactt catcgatcgt
     1621 tcgaggtctc atgcgataag ccaatggctg ttgcatagga ttttcctcct tcttgttttt
     1681 acgtccctta attatatcat atttagcaag atgcctagaa agaacagcct tctgttgttt
     1741 tgttttcata gcttatccaa ctaatattgt gtatgataaa gtaggtgaga aaaatgacgg
     1801 ataaacaaaa gatagatgaa tttttttgga ataaaacgac gccagaagtg gcacgggatt
     1861 tattggggat gtatttggag catgacacac cagaaggaag attagctgga tatattgtag
     1921 acgcggaagc atacctaggt ccagaagacg aggccgccca tagctatggt ttaagaagaa
     1981 caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat ctacctttac acgatgcata
     2041 ctcatcggat tttgaacatc attactcagc cagaagggat tcctcaagga gtaatgatac
     2101 gtgcaatcga acccgccgca atgattgatc aaatgagtaa aaatcgtggc ggaaaaactg
     2161 gtccagatat cagcaacggt ccgggaaaat tagtggaagc attagcaatt cctcaagaac
     2221 tttatggtca gtctatagca gatagttcgt tacgcttagt ttttgagaaa aaaaagacac
     2281 caaaaaagat caatgctttg cctagaatcg gtatcccaaa caaaggtgta tggacagaaa
     2341 agccgttgcg ttttgttgtg tcgggaaatc cttatatctc tttacaaaga aaaaaccaaa
     2401 tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga aggatctatt aacatattta
     2461 gatgaaataa ttgaaaaaca agtgacagac tatgatgtag ctttagattg ggatacaaaa
     2521 aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa ataaagcaca atttgagatt
     2581 gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg aatttgaaga cggcgtactt
     2641 ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt atctagcagt aatcccttat
     2701 gaaggaaaaa aagggttggc aaaatcagta gctgattctt tagtggaata tctaaatgaa
     2761 gtattagctc aagggcaaag cgatttgctt gattttttgg atgaagacga tgacgaagct
     2821 gtattcgagc ttcactggga taatcagcaa ctagcagaat tagtagaagt aaaacagcaa
     2881 aatgaagcag atacttattt accgtaccca agttattaag gagtgacaga tatgaaatgg
     2941 aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag cagttgctaa tattttgatg
     3001 gaggcagggg cgagtggtgt cgcgattgaa gattctttag atgttgaaca ttttcagtcg
     3061 gatcaatatg gtgagatttt agataaaaat gattttacat cgctgaaaga aggcgcacta
     3121 gtcatggctt attttccgga aacgactttc ttaccggaaa tcttgccttt tatcaaagat
     3181 cgtatcacac aattacctga gtttggtcta gctattggaa aaaataaagt aacagttagt
     3241 gaagtagagg aaagcaactg ggcaacagcc tggaagaaat actatcatcc ggtgcgtatt
     3301 acgcgaatgc tgacggttgc gccaagctgg gaaaactacc agacagaaga tcctctagaa
     3361 aaaattatca cactagatcc tggtatggca tttggcacag gcactcatcc cacgacttcc
     3421 ttgacattga cagcactaga aacagtcttg cgtggtggtg aaacattgct agatgtggga
     3481 actgggtcgg gcgtgctaag tattgcagca aaatatcttg gtgcaaaaga tgtctatgcc
     3541 tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa atatggatat gaatgaaata
     3601 gccaaagatg tccatgtatc agctaacgat cttttgacag gaatcgagat tgaaagtgat
     3661 gtaatcgttg cgaacatttt agcagatatc atcttattga tgatcccaga tgcttggcgt
     3721 ttactgaagc aaacaggcac gctgatcgtt tcaggaatca ttgaagagaa gaagcaaatg
     3781 gttctggatg ccatgttaga acaagggttt atcgtggatc aaattttcca gcaaaaagac
     3841 tggtatgcaa ttattttgaa aaaaccagag gtggaataga tgcagcggta ttttttaaaa
     3901 gaatcatatg aatcaaaaga ctattttgtt ttagaagggg aaaactatca ccacattgta
     3961 cgggttatga ggatggaacc cgaggatgaa gtttttttgg cattcaatga tcaaatagcc
     4021 attgttgcag aaatcacgga gattactgaa gaggctgttt atctgaaaga aattaaaaaa
     4081 gaagaaaatc aaaaggaact gccaagttcg attaccatag cgagtggcta tcctaaaggg
     4141 gaaaagctgg attggatcgt tcagaaatcc actgaattag gagcgcaagc ttttcttggt
     4201 ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa aacgtatgaa acgacaacaa
     4261 cggcttgaaa agattgctca agaggcagcg gaacaatccc atcggcagat cgtgccatca
     4321 gtagagttat tggaaacgaa acaagattta tttgagcggc tagatgatta tgacgtggtc
     4381 ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat caaatgcagc caaaattctt
     4441 agtgaaacaa aaccagatag tcgaatctta gctgtctttg gaccagaagg tggattatcc
     4501 cgcgatgaag tcgattattt tgtagaacac aaagggatac tttgtggatt aggaccaaga
     4561 atccttcgta cagaaacagc acctctttat ttattaagtg ctgctagcta tcaatgggaa
     4621 ttaatgaatc cttctttcag ctaacgttct tcattaataa aagagtatcg acagaaagaa
     4681 ttgaaaaaat agtcaacaat ttgtataata gaaacaatta tttatacaat ggatacgaaa
     4741 aatcgtttga aaaagctttt aaaaagcgaa catttccgta aaaaaacgta taactgcacg
     4801 gattttggga caattatcaa acagatattt gtcccgattc ccgttttacc gaaaaaagaa
     4861 agggggatcg attcctgtga caaaagaaga aattttaacg ggacccggtg tcataaagct
     4921 cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt gagaaagcat gcgaatacgc
     4981 gactgctgca catgacggac agtttagaaa atcaggcgaa ccctacatta ttcatcctat
     5041 ccaagtcgca ggtatattag cagatttaaa aatggatccc catacagtgg ctacaggctt
     5101 cttacatgat gttgttgaag atacagaaat cactttagaa gatctgagag aagaatttgg
     5161 cgatgacgtt gctatgttag tggacggcgt aaccaaatta gggaaaataa aatataaatc
     5221 ccacgaagag cagctagcag aaaaccaccg aaagatgctg ctagcaatgg ctcaagattt
     5281 acgagttatc atggtcaaat tagctgatag actacataac atgcgtacgt tgaagcacct
     5341 gcgagaagat aagcaaagaa ggatcgctca ggaaacttta gaaatctatg cgcctcttgc
     5401 acatcgtcta gggatcagcc ggatcaaatg ggaattagaa gatacagcac ttcgttatct
     5461 aaatccaaaa cagtattacc gtatcgtcca cttgatgcag acgaagagag aagaacgtga
     5521 aaaatacgta agtggtactg ttgaagatat tcgaatagcg acagaagagc tggggatttt
     5581 tgcagaaatc tatggacggc caaaacacat ttattcaatc tatcgtaaaa tgaaagatca
     5641 gaaaaagcag ttcaacgaaa tttatgactt gctagctatt cgagtgatcg tagattcaat
     5701 caaggattgt tatgctgtat taggagcaat ccacacaaaa tggaaaccaa tgcccggcag
     5761 atttaaggat tatattgcca tgccaaaagc taatatgtac caatctttgc atactaccgt
     5821 gatcggtccg gcaggaaatc cggtagaaat tcaaatccga acacaggaaa tgcatgaaat
     5881 cgctgaattc ggggttgctg cacactgggc ctataaagaa ggaaaaaatg aaaaagtaga
     5941 accagatggt atgacgaaac aattaagctg gttccatgag atactcgaac ttcaagacga
     6001 aagctatgat gcttctgaat ttatggaagg cgtaaaagga gatatcttta gtgataaagt
     6061 ctacgtcttc acaccaaaag gagacgttac tgagttacca aaaggatccg gaccattgga
     6121 ctttgcatac agtatccata cagatatcgg taacaaaacc actggtgcaa aagtaaatgg
     6181 caaaatggtg cagcttgatt acaaattgaa aaacggagat atcattgaga tcatgacttc
     6241 tccaaattca tttggcccaa gtcgcgactg gttgaaatta gttgctacta gcaaagcaag
     6301 aaataagatc aaacgtttct tcaaagccca agatcgagaa gaaaatgtga tcaaaggcca
     6361 cgaatccgtg gtcaaatgta ttacagatct aggatttacg cctaaagata ttttgacgaa
     6421 gaacaaactg caagaagcac tcgatcgttt taattatcaa acagaagatg atctctatgc
     6481 agctgtaggg tatggagaag ttagcccctt gacgatggcc aatcgtctga ctgaaaaaga
     6541 acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca gaagaaatca tgaatcagcc
     6601 gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt ggcgttgtca ttcaaggggt
     6661 agaaaactta ctaattcgta tcagtcgctg ctgtaatccg attcctggtg atgatatcgt
     6721 tggttatatc actaaaggca gagggatatc cattcatcgt cgagattgtc cgaatgttca
     6781 gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc gaatgggaag atacatcgaa
     6841 tacacgaaaa gagtatgatg cagatttgga aatttacggc tataatcgtt caggcttatt
     6901 gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat ctcaacagcg ttgaagcacg
     6961 gacgaataaa gataaaatgg cgacgatcca tttgacggtt ggtatccaga atttatccca
     7021 cctaaagagt atcgtggata aaatcaaagc agtacctgat gtctacagtg tacgccggac
     7081 gaatggatag gaggacagat atgagagcag ttgttcaacg ggtaagtaaa gctgctgtgt
     7141 cagtcgatca gcaagaaatt ggaaaaattg gctacggcct acttgtttta ttaggcattc
     7201 atgaaaccga tacgcaaaaa gatgtggact atctggtaaa aaaaattgca caaatgcgta
     7261 tttttgaaga tgaacaagga aaaatgaata ttagtgtaga agatgtggga ggacaaattt
     7321 taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa aggaaatcga ccaagctttg
     7381 tggaagccgc acgaccagaa acagctattc ctttatacga atcattcaat gaaggaatca
     7441 gaagtcgcgg catcacagtc gctaccggac aattcggagc agatatgtcg gtttctctcg
     7501 tcaatgatgg acctgtaacg atcattattg attctaaaaa caaataaagt aatctggttt
     7561 tcactagtaa aaactatgaa acacgtcgct taagttagac tgataaactc taacttaagt
     7621 ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt cggaaaaaaa gttaagctta
     7681 aattatacaa gttatttttt ataggaggtt gctgaagatg aaagaagtac cagtagtaga
     7741 tatcaaaatc acagatttaa aaaaattgta tcaaaaagga tacaacatgt tggaggagtt
     7801 acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt ttcaataaag aagctatcac
     7861 gatctacgga tcatcagcag caaaagtatt ttatgatcca aggaatttca aacgaaaagg
     7921 tgctatgcca aaattagtat tgaagacact ttttggtcaa ggtggagtac agactttgga
     7981 tggagctgct catcaccatc ggaaaaatat ttttatggat ctaatgacac cggaaagaat
     8041 ggaagattat catcgtatct tagataagaa tctgacacaa gcacttgaag cacagcatgg
     8101 tcaatttgaa ctatttgatt taagtaagat ggttttcttt acatctattt gcgagtgggc
     8161 aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa aaattagcag aatatcaaat
     8221 ctctatgatc agcggaacgt ttacttctcc gattgatcac ataaaaggtg tcgaaaatcg
     8281 caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa gctcgacaaa atcctgtggc
     8341 tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc acagatttag atggtcagct
     8401 tttacctctg gaagtggcag ctgttgaatt attgaacatc atccgtccaa cggttgctct
     8461 aactgtgtgg gcggcattga tgggacatgc acttttcagt agaccagatt tgtatcagca
     8521 attaaaaaat gacttttcga cgttacaaga tccgtttatt caagaaatga gacgctatta
     8581 tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa gtagaagtag atggttatcg
     8641 tataccagaa ggcagttggg ttattttaga tttatatgga acagaccatg atgaacgaac
     8701 agttgaagcg ccagattcct ttatgatcaa acggtatgta gggaaagcaa aagatatttc
     8761 ctataaagaa gaatatgaga tgattgcaca aggcggggga aattttcgac aaatgcatcg
     8821 ctgtgcagga gaatggatca ctcttcatag tctacgtgtg ttttccgatc agctagtaaa
     8881 taagtttgag ttctctgtac ctgaacaaga ttggacgatt ccattcaatc agtttccgac
     8941 ttatccgaat agccgagcgc tactttacaa gaattagatt agcataaact tatcgaaaaa
     9001 aaggaagatc aaacagtgct gatcttcctt ttttcatatt actataaatt gataaggtca
     9061 attgatcaaa atccacgaaa tgcttcatca atggtttgat aatcttcatt ggataaatgc
     9121 aaatctaaag cttggacatt gtcagatact tgctctggac gcttagctcc aggtataact
     9181 acagacactc gtggattctt catgtaccaa gctaaaacaa gttgcgcagg agtggcttgg
     9241 tatcttttag caatggggcg aagtacatcg acagcggtta aaatttctct gaaacgttct
     9301 ccttggaaat ctggatttct ttttcgagga tctccttcac caaattgttt ttcctcacca
     9361 agttcatatt ttcctgtcag caaaccggaa gcaagaggaa aatagggaac aaaactgatt
     9421 ttgttttttt caagataagg gaataattct ttttcagcct gccgatggat caaactgtat
     9481 ttatcttcta ctacatcgac atatccatct gcattcgctt ctttcaattg ttctaacgtg
     9541 aagtttgaca caccgactgc tcggattttt cctgcttctt ttaattcgtg caatgtagca
     9601 actgattcat tttttggtgt gctttcatcc ggaaagtgga tataaaaaat gtcgatgtaa
     9661 tcagtttgca gacgttttag tgcatcttcc accgattgtt tgagaaactc aggagaatta
     9721 tcaaatgttc gccctttatt tggtacatga gcagcttttg tagcgatgac gacacgtgag
     9781 cgatcatatt ctttttcctt cagtacctcg ccaatcagtt cttcggaacg gccgtttccg
     9841 taagcataag cggtatcgat caaattgatc ccgctattca aagctgtacg aactacttgt
     9901 tttcctgtct catcttccaa accgtcaaaa agattatgtc ctccaacggc atttgctcct
     9961 aatccaagct gttctgcgta gacttgtgaa tgtccgattc ttatttctgc catagctgat
    10021 tcctcctttt tgtctagtct aaaacaaaat accaaaaagc tgaactaaaa cgtttttgtg
    10081 aggggaaata gtcagatatc tcttgacttt gagactggtt tttagtaggt tataagtaat
    10141 atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt tacaagagag gatcatcgag
    10201 gctgaaaatg atctaaagtt tcattagcct aaagacactc tggagatgga cgagtgaaaa
    10261 gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga aatctttcaa aattaggtgg
    10321 taccgtgtat gaatttcatt cacccttgtt catgatgaac aagggtgatt tttttattta
    10381 catagctaag aaagggagag aaaactatga gtttccaaag accaaaagga acaaatgata
    10441 ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga aacagcacgt ttattattca
    10501 gagattatca gtataatgaa atgcgtacac cgatttttga acactatgaa gtgatatccc
    10561 gaagtgtagg ggatacaacg gatatcgttt caaaagagat gtatgatttt tatgacaaag
    10621 gggaacgcca tgtgacgctt cgaccagaag gtacagcacc aatcgttcgt tcttttgttg
    10681 agcataaatt gttcggccca gagtatgcga aaccgttcaa aactttttat atgggaccaa
    10741 tgtttcgcta cgaacgccct caaaaaggac ggttacgtca attccatcaa atcggagtag
    10801 aagcatttgg atcagaaaac cctgcaactg atgtggagag catggtgatg gcacttgatt
    10861 tcttcaaaca gttggggatc acgcaaatcc ggctagtcat caattcatta ggtgataaag
    10921 aaacgcgtca agcctatcgt caagcattga tcgactatct tttacctttt gaatcagagc
    10981 tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt acgcgtattg gacagtaaag
    11041 acaaacgaga tcaaaaattt gtggcagaag ctccatctat cttagattat ctaagcgagc
    11101 ctgctcaaaa acattttgat accgttgttt ccatgctgga tgcattggct gttccttatg
    11161 aaatcgacag taatatggtt cgcggactag attattacac acatacgatt tttgaaatca
    11221 tgagtgacgc accaaaaatg ggggcacaag caacgatttg cgcaggcgga cgttatgaca
    11281 atctagtgga agaactaggt ggtccttcaa cacctggctt cggatttgca atgggtatcg
    11341 agcgattact cttgactatg gaggcagagg aagtagttat ccctgcattt aatgagttgg
    11401 atgcttacgt ggtcgcactc ggagacgaaa caaatatcga agcattgaaa gtggtccaag
    11461 cgattcgaaa ctttggtttt tcagctgacc gcgacttcat gaaccgtaaa gcaaaagcgc
    11521 aatttaagac agcagataaa gcgaatgcca aattagtatt ggtgatcggt gaagatgagc
    11581 ttgctagtca aacgattaat gtcaaagcaa tggcaagccg aaaagaaaaa agttttccat
    11641 tagaagagat atatgaacat tttgatgaag tctatgatga aatgacttta ttcgagtagg
    11701 aggaaaaaaa taaatggcac aaagaacgat ctattgcgga ttagtttccg aacaacaatt
    11761 agaacaaaca gttacattaa aaggatgggt acaaaaaaga cgagaccttg ggggagtaat
    11821 ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc gtctttaatc ctgaaatcaa
    11881 caaagaagca tgggaaatcg cggataaatg tcgcagtgaa tacgtgatcg aagtgactgg
    11941 ggaagttaga agacgcggag aaaatgcagt aaatcctaaa atggccactg gtgaattcga
    12001 agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa accccgccat ttcaaattga
    12061 agatgatcaa gcaatcagtg acgaaatacg tttgaaatat cgttatttgg atctgcgtcg
    12121 tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca acaaaagcga ttcgtcatta
    12181 tttagatgat aatgactttc tcgatatcga aacaccttac ctagggaaat ctacaccaga
    12241 aggcgcaaga gactacctag ttccttcacg tgtccatgct ggacatttct atgcgttgcc
    12301 gcaatctcct caattgttta aacaattgct gatgaatgct ggattcgacc gttattatca
    12361 aatcgttcgt tgtttccgcg atgaagattt acgtggagac cgtcagcctg aatttacgca
    12421 agtagatatt gaaacaacgt tcttaacagc agaagaaatc caaacttaca cagagggatt
    12481 gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc acattgccat ttcctcgtat
    12541 gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa ccggatacac gttttgatat
    12601 ggaattgatc gatctaagcg atacagtcaa agaagtagaa ttcaaagtct tccaaatggc
    12661 tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa ggcgctgcag atcgctactc
    12721 acgaaaagac atggatcagt taggtcaata tgtaggacaa ttcggagcta aggggcttgc
    12781 atggctgaaa gtagaagaag acggattgaa aggtccaatt gctaaattca tgggagaagc
    12841 aacagaagca atcattaaag caacagatgc aaaaccaggt gacttgctga tgtttggcgc
    12901 agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc cggacacgtt taggaaaaga
    12961 attaggacta attgatgaat cgaaattcaa tttcttatgg gtaactgatt ggccacaatt
    13021 tgaattttca gaagaagaag gtcgatatgt ctctgctcac catccattca cgatgccaaa
    13081 agaagaagat atcccgttat tggctgaaga ccctgcaaaa gtctatgcag aagcttatga
    13141 tatcgtcttg aacggttatg aactaggcgg aggttctatc cgtattcata ctcgtgagct
    13201 tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa caaatggaag aacaatttgg
    13261 tttcttgtta acagctttag attacggatt ccctccacat ggcggtattg cattaggatt
    13321 ggaccgtttt gcaatgttat tagccggaga agataatatt cgtgaagtca ttgcctttcc
    13381 taaaaacgga aaagcagcag atgtgatgag tgatgcacca agtatggttt cgcctttaca
    13441 attgttcgaa ttgaatattg atgtaacgaa tatagaagaa tagccgataa aaaggtaaaa
    13501 aggatttgtc gaatacgctt cttttgacaa ccttgattta attgaaaaac actttataat
    13561 gaagccataa catataaagc ggggcttttc catgaaaaaa acattcgaaa attgggctta
    13621 tcatgattac acgaccaagc tttcggtatc tatcgtttat gcgttgatcg catcagtcgc
    13681 attgaatttc ttttatcagc caggacatat ttattcaagt ggtattactg gtctagctca
    13741 gattttgacg actttgtcaa aatcactgat aggattcgaa gtaccggtat caacaacttt
    13801 gtatttactg aatatacctt tatttgtatt ggcgtggtta aaaatcggga agaagtttac
    13861 aattttcact tttatcacag tcactttgac ctccctttgt atccatattt taccggaaac
    13921 agtcctatct accgatccaa tcatctgtgc gttgtttggg ggggctgtga tgggactagg
    13981 tattgggcta gcgttgagaa acggcctatc atcgggcggt ctagatatcg tgagtatcac
    14041 gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg atctacttca atgcattgat
    14101 cgtttttgtt gccggaattc tatttggctg gcaatatatg ttttacagta cgttgtctat
    14161 tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa caaaagaaga tgcaggtaat
    14221 gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc caaaactgta tgcgtagagg
    14281 gatcacgatc atcaatgaag cagaaggtgc ttataagcat gataagcaga cagtattgat
    14341 gacgatcgtt acacgatacg aactgcctct acttagagaa gcaatgaaag cttctgatcc
    14401 acaagctttt gtaagtattg cagaaaacgt ccagatactt ggtcgtttct atgaggatgg
    14461 catttagatc gagtttgaca aaaattcatg aaatactgct gtagacagac gttcgtttgt
    14521 ctgcagtttt tttctaccat agtagaatca gcaaagaaaa actgc
//
LOCUS       DAZUKA010000070        14426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000070 DAZUKA010000000
VERSION     DAZUKA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.070"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..1786)
                     /locus_tag="ACG8PN_001699"
     CDS             complement(602..1786)
                     /locus_tag="ACG8PN_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4861841.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1811..2818)
                     /locus_tag="ACG8PN_001700"
     CDS             complement(1811..2818)
                     /locus_tag="ACG8PN_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4861842.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2990..3349)
                     /gene="comGG"
                     /locus_tag="ACG8PN_001701"
     CDS             complement(2990..3349)
                     /gene="comGG"
                     /locus_tag="ACG8PN_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4861843.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3321..3746)
                     /gene="comGF"
                     /locus_tag="ACG8PN_001702"
     CDS             complement(3321..3746)
                     /gene="comGF"
                     /locus_tag="ACG8PN_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4861844.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3709..4056)
                     /locus_tag="ACG8PN_001703"
     CDS             complement(3709..4056)
                     /locus_tag="ACG8PN_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861845.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(4028..4498)
                     /gene="comGD"
                     /locus_tag="ACG8PN_001704"
     CDS             complement(4028..4498)
                     /gene="comGD"
                     /locus_tag="ACG8PN_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4861846.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4473..4751)
                     /gene="comGC"
                     /locus_tag="ACG8PN_001705"
     CDS             complement(4473..4751)
                     /gene="comGC"
                     /locus_tag="ACG8PN_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4861847.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4748..5797)
                     /gene="comGB"
                     /locus_tag="ACG8PN_001706"
     CDS             complement(4748..5797)
                     /gene="comGB"
                     /locus_tag="ACG8PN_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4861848.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5754..6683)
                     /gene="comGA"
                     /locus_tag="ACG8PN_001707"
     CDS             complement(5754..6683)
                     /gene="comGA"
                     /locus_tag="ACG8PN_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4861849.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6815..8569
                     /gene="ade"
                     /locus_tag="ACG8PN_001708"
     CDS             6815..8569
                     /gene="ade"
                     /locus_tag="ACG8PN_001708"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4861850.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="ACG8PN_001709"
     CDS             complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="ACG8PN_001709"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4861851.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9399..10181)
                     /locus_tag="ACG8PN_001710"
     CDS             complement(9399..10181)
                     /locus_tag="ACG8PN_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4861852.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10385..11596)
                     /locus_tag="ACG8PN_001711"
     CDS             complement(10385..11596)
                     /locus_tag="ACG8PN_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861853.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11589..12326)
                     /locus_tag="ACG8PN_001712"
     CDS             complement(11589..12326)
                     /locus_tag="ACG8PN_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861854.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12464..12898
                     /locus_tag="ACG8PN_001713"
     CDS             12464..12898
                     /locus_tag="ACG8PN_001713"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4861855.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12891..13235
                     /locus_tag="ACG8PN_001714"
     CDS             12891..13235
                     /locus_tag="ACG8PN_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4861856.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13415..14425
                     /locus_tag="ACG8PN_001715"
     CDS             13415..14425
                     /locus_tag="ACG8PN_001715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4861857.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4588 a         2936 c         2434 g         4468 t
ORIGIN      
        1 cgagtaattt tctagtatga ttatccattg agagactcct ttggtagggt gacgttttgg
       61 tcgactaaat tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaaagtgtta
      121 gtagaaaatc gctttcgatt ttccaccaac acaaaatatt atagaaccgt tttttcgcaa
      181 caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat
      241 caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta aacataaagc
      301 gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt tttacatatg
      361 taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga aaatcaataa
      421 ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc taagaagatc
      481 ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa
      541 aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg aatgctttgg
      601 cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct gttgggatca
      661 ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca cgcaattcat
      721 tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt tcactacgta
      781 catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca ccattcatcg
      841 ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca taagctaaac
      901 gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac aatcctgtta
      961 ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata tcaatctcta
     1021 atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt gtacccattg
     1081 tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct ccatcgactg
     1141 ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct tcgatcggtt
     1201 tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca tggaagccat
     1261 attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa taattttgct
     1321 ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg tcaggtaaaa
     1381 tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga gcaaattctg
     1441 ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc ccataagttt
     1501 ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag cttaaaattt
     1561 gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt tcgatatcca
     1621 tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac
     1681 cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga tataattgcc
     1741 atttcaaact tgaactccca gcattgattg caattgtttt agacatatga ctctccttct
     1801 cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa aattccgtca
     1861 ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt acttgttgtg
     1921 cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta ttacggaaca
     1981 attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac cattttttga
     2041 tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat
     2101 ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa ctatggcctt
     2161 cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa ccgattggca
     2221 aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg tcttgatgaa
     2281 agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat
     2341 catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg tttaaaagaa
     2401 ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc ggtgcttttt
     2461 tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta tcaggagtga
     2521 gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt aattgagaca
     2581 gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga
     2641 tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga taatcatcta
     2701 ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat gcattctgta
     2761 aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct ggtaacatgg
     2821 tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc tatcgaacaa
     2881 ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa cattttttta
     2941 ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat cattctgtac
     3001 tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt gagacttcca
     3061 cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt gtgtagtaaa
     3121 aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc tgcttcatta
     3181 tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa cggtagcttt
     3241 ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca gaaaataaaa
     3301 tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata tccatcttct
     3361 ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa ttgaagctca
     3421 gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg tatcattgcc
     3481 tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc
     3541 acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa tattagccac
     3601 tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg aatcaaaaga
     3661 ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc aatcagcgta
     3721 aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat tgacaaacac
     3781 tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca tttcttccct
     3841 tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa tcattcgaat
     3901 aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct ggccaaagct
     3961 cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca acaaaacaaa
     4021 tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat tgatacgtaa
     4081 tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt tcattcccgg
     4141 ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca ggaatatcta
     4201 atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga ttctcttcac
     4261 tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc gtagaataga
     4321 ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc catgcagaaa
     4381 atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag agtgattcaa
     4441 acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat ttctgcatgt
     4501 tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct tccattattt
     4561 tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc acgactttct
     4621 tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac taatacactg
     4681 atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata agatattttt
     4741 ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa acagcggcat
     4801 aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata aaatgcatcc
     4861 agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa atcagttctt
     4921 tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct tctgatagaa
     4981 aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct tgaatcaact
     5041 tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag actatgattt
     5101 gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa taagccgatt
     5161 gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca ctctgatcaa
     5221 caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta accgctagga
     5281 tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa tgctcttgca
     5341 tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta aacaagatac
     5401 ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa tcattgcgaa
     5461 atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc agattcccat
     5521 gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt tgcgaaagta
     5581 cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa ataacaggtg
     5641 gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa ccagataaaa
     5701 gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat cctttagaat
     5761 agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca tgattttttt
     5821 actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg attggcatct
     5881 tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc ttttttcttt
     5941 gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa
     6001 acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc atctcttatc
     6061 tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa
     6121 gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc tatctctaca
     6181 ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata cattagagaa
     6241 gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac ggttttctgt
     6301 aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa agtaccgtat
     6361 aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga caaccgcaga
     6421 cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt tcttttttct
     6481 ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc atctgccgaa
     6541 gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta aaatacttct
     6601 gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact tatcatttga
     6661 tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg tctttttgat
     6721 ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt
     6781 atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa ggaaatatat
     6841 cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta agatcgtcaa
     6901 cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg
     6961 aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc tagtgccagg
     7021 attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg aattttcaaa
     7081 agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa taggaaacat
     7141 tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac tcatgaatat
     7201 cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg gtgcaaattt
     7261 aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct tagctgaagt
     7321 catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga tccaattgat
     7381 gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg aagagttaaa
     7441 tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa aagaagctaa
     7501 agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg caaaagatct
     7561 ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct ttgtcaccga
     7621 tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc
     7681 tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga atgcagcaga
     7741 atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg atttcttctt
     7801 aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa cattagttgt
     7861 tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat atacgtgttg
     7921 tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc ttctttcttc
     7981 ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag atttggtaga
     8041 aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc aattaaagat
     8101 tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag tgaaaggatt
     8161 tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata atctagttgt
     8221 cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc aaaaaggtgg
     8281 cgggatgacc gttgtcaaca atcagcagga aatagcttgt gtttctcttc ctataggcgg
     8341 aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat taataactaa
     8401 agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt ctttcttaac
     8461 attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat tttctcgctt
     8521 ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa tcaaataaat
     8581 cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat ggatattaat
     8641 gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt atcttgaaat
     8701 tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc atactccgtc
     8761 ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt gaggatcatg
     8821 ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc tgttttgaaa
     8881 tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt cttatatgtc
     8941 aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag atagatttga
     9001 tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg tagaagtttc
     9061 aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac actgacctgc
     9121 atttctactc caatataatt gatttcagga tgggcttttg ccattccagt gatgaactgg
     9181 cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa ttcattccat
     9241 tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc taacatttcc
     9301 gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc tctaattaaa
     9361 aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa aattcgtttt
     9421 aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata tctggtgatt
     9481 tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc gtcagtcggg
     9541 cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata atttcctaaa
     9601 atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc gactagatat
     9661 aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac tactgataag
     9721 ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat ttgcagaaat
     9781 tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat cttctccggc
     9841 gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga atgatgtaaa
     9901 tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt cagcaagcga
     9961 ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca
    10021 agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta
    10081 ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg
    10141 ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc cgtcctctta
    10201 ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc aagcacatat
    10261 acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg
    10321 aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct acacagcttt
    10381 gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca aagagcatag
    10441 taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat acgactgcag
    10501 caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga tactgaatcg
    10561 cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata tagcggaact
    10621 ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa ccgataatca
    10681 gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt actaatcgga
    10741 aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag taaagataag
    10801 tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt cttcttttga
    10861 tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag atctgatgca
    10921 acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct tgttgcttcc
    10981 acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct gcccatatta
    11041 gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct aatttccaat
    11101 aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc agatgactga
    11161 atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat tgctccgtac
    11221 taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc aacggaaaaa
    11281 tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct ttaggaagta
    11341 aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat aaacagagca
    11401 tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg gttttcaacc
    11461 aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa acaaaatgat
    11521 cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct aaacgttttt
    11581 gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc gtccaaagaa
    11641 gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc tcgtatctct
    11701 ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa aatatgcgta
    11761 gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag caatgcattg
    11821 atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga aggctcgatc
    11881 aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt agctgggaac
    11941 cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct tttcattgct
    12001 atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct caatgtcaat
    12061 tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata ttcttccgta
    12121 tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc cagcagaccg
    12181 ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa ccctactatc
    12241 tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc gtacccccct
    12301 gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt tatttattat
    12361 accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa cttatggtaa
    12421 agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa actgtatttt
    12481 ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg acaaggtcta
    12541 tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca
    12601 tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt cgcgtgtgcc
    12661 taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta atatcttgaa
    12721 taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt tagtccctcg
    12781 ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata gttatggatc
    12841 ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa
    12901 actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt ctgttattgg
    12961 cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa gaagcaacaa
    13021 ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg atcgaaacaa
    13081 aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa gatatcgaag
    13141 attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa
    13201 aagctgatct gccagagtat cctgaacaaa tataataatg attattttct caacagattt
    13261 aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt
    13321 gtttgaaact accacgacaa cgtttttaaa ggaaatggga tatggtatag ttaatatata
    13381 aaaataataa ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc
    13441 agctactagt gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga
    13501 tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact
    13561 tgaatcatcg aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa
    13621 atatggcgat aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc
    13681 attaggtgat acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa
    13741 agaatatatt cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac
    13801 agatgacgat ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat
    13861 caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga
    13921 tttctctaaa ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa
    13981 agttaaattc gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa
    14041 attgaaagac ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac
    14101 agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata
    14161 caaagatcaa ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac
    14221 taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga
    14281 aaatgtgttg agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac
    14341 agcttcaaca aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga
    14401 agaaacaact gattcatcaa aataaa
//
LOCUS       DAZUKA010000071        14129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000071 DAZUKA010000000
VERSION     DAZUKA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.071"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG8PN_001716"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG8PN_001716"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4861858.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG8PN_001717"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG8PN_001717"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4861859.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG8PN_001718"
     CDS             8118..9086
                     /locus_tag="ACG8PN_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4861860.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG8PN_001719"
     CDS             9104..10009
                     /locus_tag="ACG8PN_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861861.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG8PN_001720"
     CDS             10006..10812
                     /locus_tag="ACG8PN_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4861862.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG8PN_001721"
     CDS             10969..11937
                     /locus_tag="ACG8PN_001721"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4861863.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG8PN_001722"
     CDS             complement(12254..12778)
                     /locus_tag="ACG8PN_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4861864.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACG8PN_001723"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACG8PN_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4861865.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3996 a         3046 c         2791 g         4296 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZUKA010000072        13906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000072 DAZUKA010000000
VERSION     DAZUKA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.072"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..513)
                     /locus_tag="ACG8PN_001724"
     CDS             complement(<1..513)
                     /locus_tag="ACG8PN_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861866.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRD"
     gene            complement(1081..2016)
                     /gene="whiA"
                     /locus_tag="ACG8PN_001725"
     CDS             complement(1081..2016)
                     /gene="whiA"
                     /locus_tag="ACG8PN_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4861867.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2050..3048)
                     /gene="yvcK"
                     /locus_tag="ACG8PN_001726"
     CDS             complement(2050..3048)
                     /gene="yvcK"
                     /locus_tag="ACG8PN_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4861868.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3045..3929)
                     /gene="rapZ"
                     /locus_tag="ACG8PN_001727"
     CDS             complement(3045..3929)
                     /gene="rapZ"
                     /locus_tag="ACG8PN_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4861869.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4064..4795)
                     /locus_tag="ACG8PN_001728"
     CDS             complement(4064..4795)
                     /locus_tag="ACG8PN_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4861870.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(4799..6064)
                     /locus_tag="ACG8PN_001729"
     CDS             complement(4799..6064)
                     /locus_tag="ACG8PN_001729"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4861871.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6327..9143)
                     /gene="uvrA"
                     /locus_tag="ACG8PN_001730"
     CDS             complement(6327..9143)
                     /gene="uvrA"
                     /locus_tag="ACG8PN_001730"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4861872.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9153..11147)
                     /gene="uvrB"
                     /locus_tag="ACG8PN_001731"
     CDS             complement(9153..11147)
                     /gene="uvrB"
                     /locus_tag="ACG8PN_001731"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4861873.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11406..12908
                     /locus_tag="ACG8PN_001732"
     CDS             11406..12908
                     /locus_tag="ACG8PN_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4861874.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12910..13647
                     /locus_tag="ACG8PN_001733"
     CDS             12910..13647
                     /locus_tag="ACG8PN_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4861875.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         4018 a         3012 c         2395 g         4481 t
ORIGIN      
        1 atcccttcta gtaatgcctc tttccctttc aatgaaagaa gcactaggaa aagtatgatt
       61 ataaccattc aacctatctc ccatgtaata attagcaggt cctggaaaat aaaatatttg
      121 cttattatca ctaacttcta acagcaacac taaatcttct ccgctggcta aacgacttgt
      181 ccatctacga tatctcccta tgccttcgtt cattccaaat ccatcaaatt caactattaa
      241 cgtgtcttct atcacatgta attcttcatt caattcatac tcaagtctaa aaggaaattc
      301 tccatatgta atctctggca atggtggtgc tggagttctc caaataaata caataactgt
      361 gaatagaaaa aatccaacga tcgatataaa tgttatgaga cctgctcgaa gtaaaacccg
      421 atataataaa aatgctgtat ttcgctttgc tatatctaga aaaataaaaa gtactgcaat
      481 cataatgata gtaaaagttg taaaaaaacg catttttgcc ctccctttat aaacatcgat
      541 tctcaaattc taaaccaaat cttggtagtt caagttcagg tataacgtat tccaaccata
      601 catctcaatt actatttatg aacttatgat tatcactgaa taaatcactc catccccttg
      661 ttaaaacttc tacattaatt acacctggtt gttttcgact agctaatcca atgaacttat
      721 caaccatcct tatccaccaa ccacgagaat cttgaccaga agtaagaaga gcagttctca
      781 aggatgatcg agcagttcct tccagttttt taattaatca ggccagttat agataaatac
      841 ttgatcttat ctatatcagg acttctatat taagcaaaaa tttatgtaca aaactaaaat
      901 cttcattcag ttgtctcccc gctttttata cgattgtatt aaaaacaata gaagcctttc
      961 caaattatgt tactccagta aagatggaat tatagtttga cggaagaaaa gcaagtataa
     1021 cttttaaaaa aaactgatac attcgcatct cctgcttcat gtatcagttt tttctttgga
     1081 ttaaactgct tcttcacgca gtttttccgc aaattcattt attttgcgaa ttcggtgatt
     1141 aattcccgat tttgatatcg cacctgaggg aatcatttcc cctaattctt tcaagctgac
     1201 ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt ttttcaggaa gggcatgtag
     1261 accgaccttt gcttcgatca attcgatgtt ttcgatttgt ttagaagccg catcaatcgt
     1321 cttgttcaga tttgctgtct cacaattgac tagacgattg accgaatttc tcatatcccg
     1381 tacgattcga acatcttcaa atcggagcat tgagttggtt gcaccaatca atgttaagaa
     1441 atcagcaatt ttttctgctc cttttaaata agaaatatag ccattgcgcc gttcaagcgt
     1501 tcgagcatgt agaccgtaat aatttaacat atcgcaaatg tcttggttat gctgttcata
     1561 aatagaaaat atttccaaat gataacggct cgtttccgga ttattgactg aacctgaggc
     1621 cataaaagca ccacgtaagt aagaacgcat tttttgcgaa tttcccataa tctcgtccga
     1681 aacgtgtcca ttgaacatca taccatccat gatatcaaga tccatcaaca catcttttgt
     1741 gccttgcttc aaccggacga tatagacatt gttctttttt aatttcattt ttttacgaac
     1801 gagtagctca cttcgtacct gataatgatc tttcaaaaga gaatagatac gtctggcaat
     1861 tgcagcattt tctgtttgga cgttcaaaat aaactgctgg ttcacaatac ctaaagagcc
     1921 gttcattcgc agcaaagcag ctaattccgc tttcgcatgt tctcgatgaa cttccaaacc
     1981 agttagttct ttcttcacat ctgcagcaaa tgacatccta ttctcctcct ttctccaaca
     2041 ataaattgct taatattttg aaccgaaaac taaacggaac aattcttcta ctactttgtc
     2101 tccatcgtgg aagacaccac catctttcaa ttccaaaaag tcggtggaaa tcactcgaca
     2161 tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt actagatact catcatagat
     2221 ctctgggtcc atataatctt caggcacttt ttccgtattg accaatactg tatcgataaa
     2281 aggctgattc aaatgcttgt gtaaaacacg tatatgatcg gcatctgtga aatgttccgt
     2341 ttcccctttt tgggtcatga tattacagat atagacggtt tcagcagttg tcttgagcat
     2401 tgcttcgccg atttctggta tcactaaatt tggaaggata ctggtgaata aacttccggg
     2461 tcccaaaacg accatgtcgg cgtccataat tgctgaaacg acttttcgag ccgctttggc
     2521 ttcactttta tcatttgtgt tgcagacata cacacggtca atggttttac ggtcgatcgc
     2581 gattttagat tctcctactg cagtcgttcc gtctttaaag acagcatgca aggtcaaagg
     2641 agtttctgaa gaaggataaa catgtccatc tacatgcatc atttttgcta ataattgaat
     2701 cgcctcataa gtgcttcccc gcatctcgga aactgcggca atgatcagat taccgatcgt
     2761 atgattggct aaaaactgat cttccttttt gaaacggtat tggaaaatat cttcatagaa
     2821 ttgtggcata tccgataacg ccactaagac atttctcaag tcaccaggag gagccatagc
     2881 gatcgattct ctgattgcac cgctgcttcc cccatcatct gcaacagtca ctactgctgt
     2941 gatatctgct ccttgatttc gcaaactttt caggataact ggcagaccag tgccaccgcc
     3001 gaccactacg atctttggtt ttcttatccg gtaggttttc attttcacga ccggttcacc
     3061 gtctctttcc ttttatcctt gtcccgatgt gtcacattga ctttatagtt tttccctaaa
     3121 gcatttgcca aacgttcagt caatgccaca gaacggtgtt gaccgcctgt acagccgatt
     3181 gcaactgtca agctgctttt tccttcttta atgtatccag gcaatacagt ttccagaaga
     3241 tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg acatcacata atcatagact
     3301 ggctgatctt ttccggtcaa tggtcttagt tccgggatat aatgagggtt gggcaaaaaa
     3361 cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt atttaaaacc aaatgataca
     3421 aattcgatcc gaaatccttg gtctcccttt gatttgaatg actcattgat tttttcacgt
     3481 aattgtcgtg gtgtcaatgt cgtcgtatca atgatcacgg aagcttgggc tttcaagtcg
     3541 tctagtatcg cccgttcttt acgtatccct tcagtgatca aaccgtccat cgccattgga
     3601 tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt cgtctgaagc atctaaaaat
     3661 aagatagaag tgtcgatgaa gttcgtattt tcgatttcga tcagcatgtt ttggatttct
     3721 tcaaagaagg aacgagaacg taaatccact cctaaagcca tttttgtgac tttgcctgat
     3781 tctttgatta attcccagaa ttttggaatc aatcgaggcg gcatattatc gatacaaaaa
     3841 tagcccatat cttcaaaact ttggatagct actgttttcc ctgcaccgct catacctgtt
     3901 atgatgacta attgcaaatt atctgtcata attacgaact cctctcacat tgtgcatagt
     3961 aaaattatat cattccgggc gtatcattac cagttttctt tttcttttac aaaaagattt
     4021 ggaccaaata tcttttagga tatttgcccc aaatccaatt tttttacttt tccgatgctg
     4081 tatccaatgc cttgcttctt tcagctagcg ctcgttcaat cgtccgatct gctaaaatag
     4141 attgggcatc gatcactgga taatggttat cttcagcaaa ttcatgcatc aatgacaatt
     4201 ctgtgcaacc taagataact ttttcacagt ttaggcgctc taccgcttct tctaagattt
     4261 cgtaatatag ctcttgattc aaataatccg attccttgat ctcatgatag atcagataat
     4321 tgattttttc ttgaagaaca gtatcaggaa tcactgtttc gaatccaaga tttttgactt
     4381 cgcgttcata gattccagct ttcatacttc cttctgttcc tagaatcgct actctccctg
     4441 tcgtatgctg acggacaagt tcatttgctg cttctcttgg catatgaagg ataggaatat
     4501 ccgttgccgc ttgtaattct tcaaaaaagt agtgtgcggt gttacaagtc aacacaatga
     4561 agttcggctg caataaattt tgtttctcaa tatcatccag cagaaacggc attggatttt
     4621 cttctgatcg atctaaaata taagctgtac gatcaggaac tgttgcatga ttgaataaga
     4681 cataattcaa atattcttga tcttttgtag ctttcgttcg gtggttgatc aagcgaacaa
     4741 aactttctgt cgccatcgtg cccattccgc ctaaaatact gaaaaaattc tccatgaact
     4801 agccttcttt cacatgaaaa tactttttga atcttggaat atagttatgg aacatatatt
     4861 tcatcaacaa ccagcgcttg attgaccggt ctttttcata aaacacagta gtaccgtaac
     4921 gattttcttt gatcatttgc tcagctagtt ttttgtcttc attttcccga gcatattcta
     4981 aaaagatttt tttgggcaca cccatccata atcttgcttt atctgattga ttcgttccat
     5041 aggtcgtttc aacaaagggt ttgttaaaca ctcgatcttc tgtgacgaaa cgagccagat
     5101 tcaagccgtt caatgtcaca aagaaactgc tacgaccttg tctcaaattg atttcaaata
     5161 atttatattc tccatctcta ggatcatact tcatatcgaa attggcaaag cctgtatatt
     5221 cgattttttc caaaaatgcc ttaatggttt gatagatttt ttcgttataa tctggcatga
     5281 tcacaacgta attaccaatc gatgctggtg taggatcttc taacagtgga tgaccaagac
     5341 acatcatccg aacttgatga tcttcatcta catacgcatt tagcacacgc atattactat
     5401 catcacccgg gataaaatct tgtacaatca tttcactttt ataaccggct tcgtatatcc
     5461 ggcctaaaat taaatcgaac tcttctctag tctctaaaat aaaagctttt tttcttcctt
     5521 caaattggac agaaagatac tccacactgt ttgcaggttt caaagcaact gggaattcaa
     5581 aaggcaattc ctgttccaaa tgtccattta ctaacatttc ttctctgacg atcaatgttt
     5641 taggatacgg aagatcatat tcttcacaaa cttcataaaa gcttacttta ttgatcagtc
     5701 tttcaaataa agaataatca atatatggac agataaaata ttctgacagc tcttgtttat
     5761 gttgggagat caattctgca tatccatcac cacaagcaat caaaagatat tttttactct
     5821 tatcagaata tctttctttt gccaaacgaa gcatcgtctc gataaacaca gggtctttat
     5881 caaatcctgg aataacttct acattgacaa ttttactgta gcgggtaggg gctaactgat
     5941 ccgaagcata tgcttggcag attttgccgt atgcttcatt aaaagaacgt gccataccat
     6001 aaacgttcat gtcgcttcct aataatatag ggataaattc ttcattttca atactgttca
     6061 tcattatctc tcacttactc ataatctctt gtttttcttt tccaactaca caaggaatat
     6121 cataccataa agaaaaaagc aatcaaagga aactcctccg attgctcaat tcttttttga
     6181 acggattgaa cttataggaa gtttttgtct ataccaatca gtgtagaaag aactaccatg
     6241 taatgattct agaacactga tattaataaa acaagcaaaa attcattttt ttatgttttt
     6301 ttaactatct cagctatcta tcctctttat accattattt ttttcaaata gtgacctgta
     6361 taactttctt ttacgttaat gatttcttct ggtgttccag tcgcaaggat cgttcctcca
     6421 ccttctccac cttctggtcc taaatcaatg atataatctg ctgttttgat tacatctaga
     6481 ttgtgttcaa tcactaaaac tgtgttacca gcatctacta atctttgtaa tacatgcaac
     6541 aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt catctagtat atagaaattc
     6601 tttccattag agattttgtg caattcactg gcaagtttca tccgttgtgc ctcaccaccg
     6661 gacaatgtcg ttgctggttg ccccatcgtc acataaccta agccaacatc aacaatcgtt
     6721 tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt ctacagcatc ttctactgtc
     6781 atttccaaaa tatcagcaat gctttttcct ttgtaatgca cttctaatgt ttcagagtta
     6841 tatcgtttgc catgacatac ttcacaagga acatagacat caggcaaaaa gtgcatttcg
     6901 atcttaataa ttccatcccc gcgacaagct tcacaacgac cgccttttac gttgaaacta
     6961 aaacgccctt tcttataacc ccgcattttt gcctcgttcg tttgagcaaa taacccacgg
     7021 atatcatcaa atacacttgt ataagtcgct ggattacttc tcggcgtccg gccgattggg
     7081 ctttgatcga tatcgatgat cttttcgata ctttcgtagc cggaaattgt cttgaattta
     7141 cctggcttag cagaattctt atttaatttt tgcgctaacg atttctttaa gatactattg
     7201 accaatgtac tctttccaga accagacaca cctgtgactg ctacaaattc tcctaatgga
     7261 aattcaacac taatattttt cagattgttt tctgaagcac cagtgatctt gattgctttc
     7321 ccattacctt tacggcgttc ttttggtaca gggattgatt ttttccctga aagatatttc
     7381 cctgtcaaag aattttcatc attagctact tcttcaggag tgcctgcagc cacgatttgt
     7441 ccgccttgtt ctccggcccc aggtccgacg tcaatcagat aatctgctgc ccacatcgta
     7501 tcttcatcat gttcgactac aacgagggtg ttgccaagat cacgcatttt ctttaatgag
     7561 ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg atggttcatc taaaatatac
     7621 aaaactccag aaagattcga tccaatttgt gtagccaatc tgattcgctg cgcttctccc
     7681 ccagataatg ttccggaagc gcgactcaaa gttagatagt ctagtcccac attttccaag
     7741 aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg caattacttt ctcttgttca
     7801 gataacgtta agtttgagaa gaaatctact gctttttgaa tagacaactc atttgtttgc
     7861 ccgatattcg ttccattgat ttgaacagat agagcttggg gattcaatcg ataaccttta
     7921 catgtctgac aagttaattc tgtcatataa agacgcattt gctcacgcgt gaaatcactg
     7981 ttcgtatcat gataacgacg ctcgatattc ttcaataccc cttcgaaagt cgtttctaca
     8041 tcgcgaacgc caccaaaatc attttcataa tggaaatgga aaagatcatc tgtcccattc
     8101 aagatgatat cctgatgctc ttccggtaat tcttcgaatg gtgtatctaa atcgataccg
     8161 aaactttcac aagcctgcgc taacatttga ggataatact gagagctgat tggattccat
     8221 ggaacgatag caccttgact aagtgtcttc gttctatctg gaatcactaa atcttgatcg
     8281 acttctaatt tgacacctaa accatcacag tctggacaag ctccaaaagg cgcattgaat
     8341 gagaacagtc ttggttctag ttcgccaaca gtaaatccgc aataaggaca ggcatagtgt
     8401 tcgctaaaca acatttcttc ttcaccgatc acgtcaacca gagcatagcc gtctgccaat
     8461 cgcagagccg cctctaaaga atcaaataga cgagaacgaa tcccttcttt cacaatgatc
     8521 cggtcgatga cgatcgcgat atcatgtttt ttgtttttct ccagctcagg cacttcactt
     8581 aaatcataga tctcaccatc gactcgaacg cggacgtatc cctctttttg gattcgttcg
     8641 aatatttttt tatgttgtcc ttttttcttt accacgatcg gtgccaacac ttggatcttt
     8701 gtgcgctcag gcaaagccat tacttgatct gccatttgat ctactgattg actggtaatc
     8761 tcgatatgat cgttgggaca aatcggatgt cctacacgtg caaacaacaa gcgtaaatag
     8821 tcattgattt ccgtcacagt acctactgtc gaccgaggat ttttacttgt tgttttctga
     8881 tcaatagaaa tcgccggact caatccgtcg atgctatcta cgtccggctt atccatttgt
     8941 cctaaaaact gacgagcata agcagaaagg ctctctacgt atctccgctg cccttcagcg
     9001 tacagcgtat caaatgctag ggaacttttt ccagaaccgg acaaacctgt aacgaccacg
     9061 aatttatctc gtggaatcgt tacatctata tttttcaagt tatgggcacg tgcaccgtga
     9121 atcgtaattt tatcgttagc catgttgtcc tcctactttg aagcttttaa ttctaaaatc
     9181 atatctcgca cattagcggc tgtttcaaaa tccaatgctt tcgccgcatt tttcatttct
     9241 ttttctaatt tcatcaataa cgtatctttt tcttctttcg tcatttcttc atacgagact
     9301 tgtaccgctt cttttgtatc atcttccgat tctttcgtaa tagaaatcaa atctcggatt
     9361 tctttaatga tcgtttttgg aacgatccca tgttcttcat tgtacttctg ctgaatcgta
     9421 cggcgtcttg atgtttcatc catcgctaga cgcatagaat ctgttacttt gtctgcgtac
     9481 atgattactt taccttcttc attccgagca gctcgtccaa tcgtttggac taaggaacgt
     9541 tcactacgta aaaagccttc tttatccgca tccaagatag caacaaggga aacttcaggt
     9601 acgtctaatc cttcacgaag caaattgatt ccaactaaca catcaaattc acctaatcga
     9661 agatctcgaa tgatttctgt tcgttccagc gttttgatat cactatgcaa atatttgact
     9721 ttgatcccta attctttgaa ataatctgtg aggtcttctg ccattttttt cgtcaatgtc
     9781 gttacaaaca cccgctggtc tttttcgact cgttcattta tttcgcctac tagatcatcg
     9841 atttgtccca tgattggacg aacttcgatc actgggtcaa gcagccctgt cggacggatg
     9901 atttgttgga taacggtatc tgtttgttca tgttcatatg gtcctggcgt cgcagagaca
     9961 taaatgatct gattgacatg tttttcaaac tcttctaacc gaagtggccg attgtctaaa
    10021 gcagatggca aacggaaacc gtaatctact agcatctgtt ttctcgctcg gtcaccatta
    10081 tacattcctc tgacttgagg catggttacg tgcgattcgt ccgccacgat caaaaaatct
    10141 tcaggaaaga aatccaatag cgtataaggc ggttcacctt ctttacgtcc gtccatatgc
    10201 cgagaataat tttcaattcc cgacgtgtag cccatttcta acatcatttc aatatcgtaa
    10261 ttggttcgct gttctaaacg ttgagcttcc agaagtttgt tttcatttcg caagacagtt
    10321 aaacgctgtt ctaattccgc tttgatattc gctacggcat gctccatatg ttcatcattc
    10381 gtcacaaagt gagtcgccgg gaagattgcc acgtgttccg tttctcctaa aacttcacct
    10441 gtcagtgcat tgacttctcg gatacgttcg acctcatctc caaaaaactc cacacgtaag
    10501 gcacgttcat ctcttgaagc cggaaaaatc tccacgacat caccgcgcac tcgaaaacga
    10561 ccgcgctgaa aatctatatc atttctttcg aattggatac tgactaagtc acgaatcaac
    10621 tgattgcgat ctagttctgc tccctgtcga atcgaaacta cttgtttttg gtactcaaaa
    10681 ggcgaaccta atccaaaaat acatgaaacg gatgcaatga cgatcacatc atttctttct
    10741 aataatgaac tagtagccga atgtcgtaat ttgtcgatct catcatttac acttgaatct
    10801 ttttcgatat acgtatcgct tgaaggcaca taagcttcag gctgataata atcataataa
    10861 gaaacaaaat attcaacagc attgttaggg aaaaattcct tgaattcccc gtacagctga
    10921 ccagccaatg ttttgttatg ggcaataatt agtgttggtt tattcacttt ctcaataaga
    10981 ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta ataagatttg tgcttttttc
    11041 ccgccgacta cgccatccac taattggtta atcgcttcag gctgatcacc agccggttgg
    11101 tatttcgaga caagctcaaa gtgtcgggag gtatctctct caatcattct tcgtcctcct
    11161 tttcattcat tccttttcat tttacgacca ataaaaagaa aaggaaagca aaggcattcc
    11221 ttactaaaaa attataccac accgaacata ctttcgccac ttttttatct tattatgcat
    11281 gttacgtttt ataacataaa atgttagaaa ctcgcatttt tttcattttt tcgagcttac
    11341 agtcttgtct gattcagcac ttttcgccta taataataaa atattggaaa tttaggaggt
    11401 tgattatgaa aagaaagcat ttatttcact catttgtttt cttaatgaca ggacttgtga
    11461 cacttgcgtt tggacatgtt gcacaagctg acaacactac aacggcagag tcaagcacaa
    11521 gcgagacaat gcaaaaaata gagcccaaaa aagacgtata cgtaattgcc agtgactctg
    11581 cttttgctcc atttgaattt caaaacgcaa agggcgagta cgaaggcatc gatgtaaaat
    11641 tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg aatggactac cgtggtttct
    11701 ctgctgcttt gcaagctttg gaatcaggac aagcagacgg aatgattgcc ggaatgactg
    11761 taacagatga acgaaagaaa agctatgatt tttctgattc ttactttgat agcgggatcc
    11821 aattagctgt caaaaaagga gatacttcga tcaagtctta tgaagatttg aaaggaaaaa
    11881 cagtcggagc aaaagttggt accgaaagtg ctgaattttt aagcgaccat gaatctgaat
    11941 atggctattc tatcaaatat tttgatgctg ctgatcaatt gtacgacggg ttaaaagttg
    12001 gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg ttatgcgatc aaacaaggac
    12061 aagaattgga aacaccgatc aaaaaagaat ctggcggaaa atatgcattt gccgttaaaa
    12121 aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg attgaaagaa ttaaaaagaa
    12181 caggcgaata cgataagatt attaaagaat atgtagcgga tggttcagaa acttccagtg
    12241 agtcttccac tgctgatgaa tcaacatttc ttggacttat ccaaaacaac tatcaagcat
    12301 tgttgaaagg actttggaga acaattctcc taactttgat ctcctttgct ttggctatag
    12361 taatcggtgt gatttttgga ttattcagtg tagcaccaat acgcgctctt cgcacaattg
    12421 ctagtattta tgtcgatatc atccgaggaa ttccaatgat ggttcttgcg ttctttattt
    12481 tctttgggtt gcctggtatc gttggattta cgattcctga cttccttgct ggtgttatca
    12541 ccttgactct taacgcaagt gcttatatcg cagagattgt tcgaggcggg atcaacgccg
    12601 ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct ttcttataat cggacaatgc
    12661 aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc atcattcgtc aatcagttcg
    12721 ttatctcatt aaaagacaca acgatcattt ccgcaatcgg tgtagtcgaa ttgctgcaaa
    12781 ctggtaagat cattgttgca cgtacaacac aaagtacgta tgtttacttg atcattgcga
    12841 tcatgtactt gattttgatc acagcattga caaaattagc aaaagtttta gaaaagaagg
    12901 tgaagtaaga tggcagaaaa aatcttagtt gagcatttag tcaaaaaata cggcgataac
    12961 acagtattaa acgacatcaa tgtgtccatc aacgaaggag atgtcgtctg tatcattggc
    13021 ccttcaggtt caggaaaaag tacatttttg cgttgcttga atcgtttaga agaaccttct
    13081 gacggtgaaa ttattattga cggcgcccac ttgatggata aaagcacaga tatcaacttg
    13141 gtgagacaac atatcggaat ggtattccag cactttaacc tattccctca tctttctatt
    13201 ttggaaaaca tcattttagc accgatggac ctgaagaaag aatctagaga agaagcagag
    13261 aaaaaagcaa tcaaattgct gcagacagtt ggtctagaag ataaaaaaga tatgtatcct
    13321 gaaatgcttt ccggtggaca aaagcagcgt gtggctattg ctcgtgctct agcgatgaac
    13381 cctgacatca tgctttttga tgagcctaca tctgcactcg accccgaaat ggtcggtgat
    13441 gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga cgatggttat cgttacgcac
    13501 gaaatgggct tcgctaaaga agtggccaat cgtgtcatgt tcattgacga cggaaacttt
    13561 ttagaagacg gaacgcctca acaaatcttt gaaaatccgc aaaatgaacg gacaaaagat
    13621 ttcttgaata aagtattaaa tatttaataa aaaccaaaaa tgcccggtcg cttcatgacg
    13681 accgggcatt tttagtttct tgttttttca cttaactgta ccagccgcat cgcttgttct
    13741 cctgcacaaa caagcaatcg ctctacctgt tccatcgcat aagaaagtac catcggttca
    13801 ttgacgatat caagtacata gtcgatacca ccttcagaaa ctatgatgat tatcttattc
    13861 taaagctggc ttttttgttg gtacaaacat gacaactaca aaaaaa
//
LOCUS       DAZUKA010000073        13888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000073 DAZUKA010000000
VERSION     DAZUKA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.073"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG8PN_001734"
     CDS             complement(146..1111)
                     /locus_tag="ACG8PN_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4861876.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG8PN_001735"
     CDS             1345..2190
                     /locus_tag="ACG8PN_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4861877.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG8PN_001736"
     CDS             2381..3568
                     /locus_tag="ACG8PN_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4861878.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG8PN_001737"
     CDS             3574..3930
                     /locus_tag="ACG8PN_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4861879.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG8PN_001738"
     CDS             3932..4270
                     /locus_tag="ACG8PN_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4861880.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG8PN_001739"
     CDS             4665..5699
                     /locus_tag="ACG8PN_001739"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4861881.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG8PN_001740"
     CDS             5692..6378
                     /locus_tag="ACG8PN_001740"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4861882.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG8PN_001741"
     CDS             6402..7250
                     /locus_tag="ACG8PN_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4861883.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG8PN_001742"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG8PN_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4861884.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG8PN_001743"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG8PN_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4861885.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG8PN_001744"
     CDS             9516..10751
                     /locus_tag="ACG8PN_001744"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4861886.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG8PN_001745"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG8PN_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4861887.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG8PN_001746"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG8PN_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4861888.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12702..13844)
                     /locus_tag="ACG8PN_001747"
     CDS             complement(12702..13844)
                     /locus_tag="ACG8PN_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4861889.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4517 a         2573 c         3003 g         3795 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tgggccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaacc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12601 ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt
    12661 tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt
    12721 gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg
    12781 gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt
    12841 tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc
    12901 ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg
    12961 tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc
    13021 attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc
    13081 tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg
    13141 tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat atcttcccat
    13201 tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg
    13261 tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc
    13321 gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata
    13381 ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt
    13441 ctcaagaaac catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag
    13501 ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa
    13561 aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat
    13621 aattcataca actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag
    13681 tctacaagta gtctattaag tgctgtttgc gcctcagctt ttgatttgaa tcttctacgc
    13741 tttgtacgta tatcttttcc agtcttggga tctttcccta aataagtttc aaatccccaa
    13801 tatttttcgc cattttgtaa tttataacta aaaaatgttg ccatttgatt ctctccttac
    13861 ttcattattg caggtagaga agttattt
//
LOCUS       DAZUKA010000074        13832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000074 DAZUKA010000000
VERSION     DAZUKA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.074"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..1425)
                     /locus_tag="ACG8PN_001748"
     CDS             complement(256..1425)
                     /locus_tag="ACG8PN_001748"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4861890.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1629..2804
                     /gene="mltG"
                     /locus_tag="ACG8PN_001749"
     CDS             1629..2804
                     /gene="mltG"
                     /locus_tag="ACG8PN_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4861891.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2940..3419
                     /gene="greA"
                     /locus_tag="ACG8PN_001750"
     CDS             2940..3419
                     /gene="greA"
                     /locus_tag="ACG8PN_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4861892.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3588..4319
                     /gene="liaF"
                     /locus_tag="ACG8PN_001751"
     CDS             3588..4319
                     /gene="liaF"
                     /locus_tag="ACG8PN_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4861893.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4316..5383
                     /gene="liaS"
                     /locus_tag="ACG8PN_001752"
     CDS             4316..5383
                     /gene="liaS"
                     /locus_tag="ACG8PN_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4861894.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5390..6022
                     /locus_tag="ACG8PN_001753"
     CDS             5390..6022
                     /locus_tag="ACG8PN_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4861895.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6162..6827
                     /locus_tag="ACG8PN_001754"
     CDS             6162..6827
                     /locus_tag="ACG8PN_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4861896.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6855..7148
                     /locus_tag="ACG8PN_001755"
     CDS             6855..7148
                     /locus_tag="ACG8PN_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4861897.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(7216..8163)
                     /locus_tag="ACG8PN_001756"
     CDS             complement(7216..8163)
                     /locus_tag="ACG8PN_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4861898.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8341..9144)
                     /locus_tag="ACG8PN_001757"
     CDS             complement(8341..9144)
                     /locus_tag="ACG8PN_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4861899.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9290..10204
                     /locus_tag="ACG8PN_001758"
     CDS             9290..10204
                     /locus_tag="ACG8PN_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4861900.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10252..10722
                     /locus_tag="ACG8PN_001759"
     CDS             10252..10722
                     /locus_tag="ACG8PN_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4861901.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10807..11415
                     /locus_tag="ACG8PN_001760"
     CDS             10807..11415
                     /locus_tag="ACG8PN_001760"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4861902.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11549..11749
                     /locus_tag="ACG8PN_001761"
     CDS             11549..11749
                     /locus_tag="ACG8PN_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4861903.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12503..13729
                     /locus_tag="ACG8PN_001762"
     CDS             12503..13729
                     /locus_tag="ACG8PN_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4861904.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4693 a         2113 c         2794 g         4232 t
ORIGIN      
        1 atattttagc aatctaaaga tactatttta gatttaatag gccctttgtc aaataatgtt
       61 ggtaaagaga aatcttagaa taatttggac agaacagaaa gaagaacacc tctttctgtt
      121 ctgtttattt ggaattaaag tctgtagcaa aaattgattt ttagatttgt cccacactct
      181 ttcctacgtt tagtcgagta ctttttttaa gcaaatgcta ttcaaagaag tagtatttgc
      241 tatcttttca tcatatcagc tatttgccaa cttagtagtt ccttccatca ggtcatagat
      301 ttggtttctt agatcagcag taggcagttt gatagttttg tcagctttta tattgctttc
      361 catgatgata gcatcctgtg aatctttgct aaatatagca gatgtatcaa aagagtcgat
      421 atttccttgc gacaggatat aattatctcc gctatatacc ccaccactat atggatgtcc
      481 attcgcattt tcacgccata atgagaagag taagtctttg gaaactagtt ttccagataa
      541 aacttgtgta aacagcgtat ttaaatcata gacggacata ctgatattac cagtccctaa
      601 ttcgttatgg atctctgttt cagattctgt cagtggttta aagtaatcat cctctttaga
      661 catttgatag gatagtgcat gattggatga atttttgact tggtcataaa attttgtatg
      721 ctgtaatcct aaaggttcga tgatatcatc ggtcaataat ttttcatacg gttgatttgt
      781 taatttgatc aaaattccag tcaatatggt aaagttcaat gaatcataat gagaggtctg
      841 ataatctgta aattttaagt gctgaatcgc aaattgaacg acttcttcat cagtcatcgg
      901 tatggttgga gaagcagttc ggtgcaagcc tgagcgcata tacagcaagt cacggatagt
      961 aatttgctgg ctgccagcaa tttgtggata gaattgagcc agcttagtat ctaaagaaag
     1021 gtgcccacgc tcgacttgtt ttaaaatcaa aagagtagtc aatgcttttt gaaccgaacc
     1081 aatctgataa taaagagaag gatcatttgg ttgttgcgtg agtttattcg catagccata
     1141 gcctttttcc atgatgatgc gattattttt ctttacgtaa attgagccaa tataatcatt
     1201 ttttcttaat agttgatcga ttttttcaac gagctgttgt tcattggtag tcagtatcaa
     1261 ctttttttga gctgattgag catgggcaac tatctgagag tgttcattat tgtaaaattg
     1321 ccatccaaga atagttcctg tagagaacgc actaaggcta aacaacacga caacgatcat
     1381 taaaaatgaa tattttcttt ttttagcatg ttttcctttt ttcataatag tttatttagt
     1441 ctcctttctt ttttacagaa agaataaagc taaaatagct tgtatcttga taacatttat
     1501 tttaatctta tagtaatgga tttactactt acatttttga tagaatattt aaataaaatc
     1561 attcacgcca attcaaatat catgtataat gaataaagtt acgaacttga tagtaaggag
     1621 gagttgtttt gtcaggaaac aaacaacacc caagtaaaga taaaaaagaa gccagtaagg
     1681 cagctacagc agatataatc gacaagcgga gaaatctgcg taaaaaagaa gataagattg
     1741 tgaggaagat cattttagtg attgctctta cattattgat tattggcgga tttcttggat
     1801 ttaccgtgta tcgttatgta gatagcgggt tgaagccttt agataaatca gatgaccaat
     1861 tagtccaagt cgagatcccg agtggctctt ctaacaagca gattggtgag attttggaga
     1921 aggacaatat tattaaaagc gggattgttt tcaattatta tacaaaattt aaaaatttaa
     1981 ctggctttca agctggctat tatcagttag cgcctaatat gactcttgac gagattggca
     2041 aacagttgca agaaggcggt acttctgagc caacaaaagt tgcagatggg aaaattgcca
     2101 ttccagaagg gtacgatatc gatcagattg ctgaacgggt agcgaaggta actggtaaag
     2161 acaagaaaga atttttagat ttagtgaatg atgagacttt ctttaacgag ttgcaccaaa
     2221 aattcccaga actgttggaa agtgcatcta aagctgagaa cacaaaatac cgtttggaag
     2281 gttacttatt cccagccaca tatgattatt acacgagtac ttcgcttaag gatctagtga
     2341 ttgaaatggt gaacaagacg aatacggtca tgcaaaacta ttattcagct atcaagcaga
     2401 aaaatttgac tgtacaagaa gttttaactc tagcttctct agttgaaaaa gaaggcgtaa
     2461 aagaaaatga tcgtaaaaac attgcacaag tcttctttaa ccgaatcaaa gcaaatatgc
     2521 cacttcagtc agatatctct gttttatacg cattaggtga acacaaagaa ttagtgacgt
     2581 atgaagatac agctgtagat tcaccatata atttgtatac caataccggc tatggacctg
     2641 gaccttttga caatccaagc gaagaggcaa tcaaggcagt attagaacca gcagaaaatg
     2701 actattatta tttcgtagca gatacgagca ctggaaatgt ttatttcgct aaaacttatg
     2761 aagaacatat ggaattagtg gaaaaatatg taaataattc ttagaaaaac aggctctaga
     2821 cattgatcat ttttagaaaa acatttacat ttgaagaaaa gcgtggtaat cttttttgga
     2881 ctatattcct caaaagatta ccttttgcgg tattttatcg aaaaaaggag acgttacaca
     2941 tggtagagaa agtttatcca atgactctag aaggtaaaga aaaattagaa aaagaattag
     3001 aagaactaaa aacagtcaaa cgcggagaaa tcattgaacg aatcaaaatt gcccgcggtt
     3061 tcggagatct ttccgaaaac tctgaatatg aatcagcgaa agatgaacaa gcatttgttg
     3121 aaggaagaat cacaacaatt gaaaatatga ttcgttttgc tcaaatcatc gataacgacg
     3181 gtgtagatgc agatgaggta tcaattggta aaacagttac attcatcgaa ctaccagatg
     3241 gagaagaaga agagtacacg attgtcggaa gcgctgaagc agatccgttt tctggcaaaa
     3301 tttccaatga ttctcctatt gcgcaagcat tgattggcaa acgtaaaaat gatgaagtaa
     3361 ctattgctac tccaggtggc gatatgttag ttaaaatcgt caaagtagaa caagcgtagt
     3421 ctgactgaaa gatctagaga gttcatctta ctgatgatac actctagatc tttttctctt
     3481 tgtgtatttt agatttcaac aaacgatctt acatctaaag tcctatagcc aacagttaaa
     3541 aaaataggta tgatgaaatg agaatgtgtc aggagggaga aagagcgatg aatagttctt
     3601 ggcgtttttt tgtggtagtt gaagcactac tgctgttatt tgctgtttgg caaatcgtaa
     3661 acaatactgg acttttgctt ttagtgatat tcggtatttt caatatatat ctagctatgc
     3721 gcaaacatcc tcgtactaaa tttcaaaatt ttcaattgat attaggaagt ttggtcattt
     3781 tttttagctt agtcaacagt cctgctttat ggatgatggc tgtatttgcc gttttattta
     3841 ttggtctgaa aggtgtagag atttcaggga tagatttaac caaaaatgct ttttggcgaa
     3901 agaaacagat catgatgatc caaacagagc aattaaaaac acataataat gagcggaaaa
     3961 gacagcaatt attcggtaac caacgtattg gaaacgacgt ctatgaatgg gacgatatca
     4021 acatcgccgt catttctggt gatactatca tagatttggg caatacgctt ttgccaaaag
     4081 atgataatat tgtgattgta agaaaaggga taggcagaac acggatctta gtacctttgg
     4141 gcgtagccat ccgattagag catgcgacat tagtaggaaa tgtcttgttt gaagaagagc
     4201 aattttcttt gaaaaatgaa caaatcaaaa tctacagcag tgattatgat gaaaaccctc
     4261 gtagattgaa gattattact aataccttga ttggagatgt cgaggtgata cgaatatgat
     4321 gggaaaaata tccagagcga tgctagctgt ttattcgggg attgcggctt tccttattat
     4381 tctattttca cttttcactt atttttatgc cagcaatcaa agtcattggt ggggagaatt
     4441 actgcgtgca cgtttattat atgttccgct tatcttccat ttgttagcca tttccttagg
     4501 tgtaggatta attgtctttc tattattatc acttattcaa aagacaaaat acgggaaaat
     4561 cgaagaaaag ctgcgtgcac tttcttctgg caattatgaa tccaaattgt tgcttcttcc
     4621 gatcccaagt gcatcggacg atttatacat caaagatatt gataaggaaa tcactaagat
     4681 aaaagaaaaa atgattgaag tatctagtga attacaaatt gtaacgagcc gtccccaata
     4741 tgtagatgga caaaccaaag aagaaatttt agaattagaa agacatcgat tagcccgtga
     4801 gctgcatgat tcagtttcgc aacaattgtt tgcagcaatg atgatgatgt cggcattgac
     4861 ggagcaagca gaaaaaagcg agacaccaga aatgtttcgc aagcagttga aaatggtagc
     4921 agagatcatc aacgcttccc agtctgagat gcgtgcgctg cttcttcatc tacgtccagt
     4981 caatttagaa gagaaaagtt taaagcaagg tatcgagcag ttattgaagg aattgcaaaa
     5041 taaaatccag atttcgctga aatgggatgt agaagatgta aaactatcta gttccattga
     5101 ggatcacttg ttccgaatcg ttcaagaatt gttatcaaat acattaagac atgctaaagc
     5161 caatgaatta gaggtatatt tgcacaaaat agacaataat cttttgttac gtattattga
     5221 cgacggaaca gggtttaata tgaatgaaac gaaaacggga agttatggat tgaacaatat
     5281 caaagaacga gtagctggta ttggtggaac agtaaaaatc attagtttta aaggtcaagg
     5341 tacaagtgta gaaatcaagg tccctttgat gaaggaggca tagataagga tgataaaagt
     5401 tttattagta gatgaccatg aaatggtgcg cttaggcgtc tctttttatt tatctattca
     5461 agaagacatt gaagtaattg gagaagctga aaatggacga caaggctatg agaaggcgat
     5521 gacacttcga ccggacgtca ttttaatgga cttagtcatg gaagaaatgg atggtatcga
     5581 atcaacaaaa gcaatcttaa aagattggcc agaagctaaa atcattatcg taaccagttt
     5641 tattgatgac gaaaaagttt atcctgccat tgaagcaggt gcagcagggt atttgttgaa
     5701 gacctctacc gcacatgaaa ttgctgatgc tataagggca acgcaacgtg gcgagcgagt
     5761 attagaacca gaagtgacaa caaaaatgat ggaaaaaatg agtcggcgaa atgagcctgt
     5821 attacatgaa gaactaacga atcgggaaaa tgaaatttta atgctgattt ctgaaggaaa
     5881 aagtaatcag gaaatagcgg acgaattgtt tattacactg aaaacagtaa aaacacatgt
     5941 ttccaatata ttggctaagc tagaagtgga agaccgtaca caagccgcta tctatgcatt
     6001 caaacatggt ttggtgaaat aaaaacgatg ataaagagga taatcaaaag ctgagaaaat
     6061 aactattatt tttcttggct tttttacaat aatgatgctt gcttttgttt ttgtaaaggc
     6121 tttgttatac tgagaaaaga agatactaaa ggaggaaaac ggtgaaacaa aattttgcaa
     6181 ttgtaggtct tggacgattt ggcggaagta tctgccgaac actgatcgaa gctggtcaag
     6241 aagtactggc tattgatagt aatgaagatc gggtcaatga atatatgaat atagccactc
     6301 atgctgttgt ggcaaatgca caggatgaaa tgacacttcg atcattaggc ataagaaatt
     6361 tcgatcatgt aattgtcgct attggagaag atattcaagc cagtatctta gttactctga
     6421 tggtaaagga aatgggcgta ccaaatattt tagcaaaagc ccaaaatgag tatcatgcca
     6481 gagtacttga aaagatcgga gctgatcgcg tcgtccatcc cgagcgagac atgggaataa
     6541 ggattgcgca taatttagtg tcaaagaata ttttagatta cctagagttg tcagataaat
     6601 attctttagc agaaatccga gtctccaatc ctaaattttt caacaaaaca ttagctgaat
     6661 taaatttcag gcaacgtttt gacttaacag tagtcgccat acgaagagta gatcaaacgg
     6721 ttatcgcttc tcctgcagca gatgaatatg ttcgtgaaaa tgataatcta ctggttattg
     6781 gagagacagg cgatgtggat attctagacg ataagatgaa tcaatagaaa gaaaagagaa
     6841 agtaggttgt tgggatgaaa ttaacggtaa cgccaaaagc acaaaagtgg tttgactcag
     6901 aaattgatct gccagaaact tacggaatac gtttttttgg aaaagtctac ggcaaaacag
     6961 aggttcatga tggtttttcg attggaatgt cagttgaaca gccagaacat cctgtaaaga
     7021 aagtggaaat tgatgggaca ctttttttta tcgaggaagc agatgaatgg ttctttaaag
     7081 gatatgattt acttgttgat tataatccca acctggagga gccaacctat cactttaaaa
     7141 aacaataaaa aaagagaggg tgacacaatt tttgtcacct tctctttttt cttttgaaaa
     7201 tattgttttc agctattaat atcctgcaga caattttact tgattggttt gaagctcatg
     7261 ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc
     7321 aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg
     7381 atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt
     7441 atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa
     7501 tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc
     7561 cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt
     7621 taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca
     7681 gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat
     7741 gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat
     7801 gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa
     7861 gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt
     7921 ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt
     7981 caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc
     8041 tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt
     8101 atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga
     8161 catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa
     8221 gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa
     8281 aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc
     8341 ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc
     8401 aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat
     8461 tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt
     8521 tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag
     8581 cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg
     8641 tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc
     8701 attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt
     8761 aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa
     8821 atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga
     8881 gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt
     8941 aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg
     9001 aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat
     9061 ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact
     9121 gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat
     9181 tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa
     9241 atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg
     9301 aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa
     9361 tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt
     9421 ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca
     9481 aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat
     9541 attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga
     9601 agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc
     9661 agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga
     9721 aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag
     9781 tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat
     9841 tacactgatc atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc
     9901 cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga
     9961 agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat
    10021 aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat
    10081 atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca
    10141 agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta
    10201 ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct catgaagcag
    10261 tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc
    10321 ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat
    10381 tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct
    10441 tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt
    10501 attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca
    10561 atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa
    10621 attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag
    10681 gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat
    10741 ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat
    10801 ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat
    10861 attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat
    10921 ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta
    10981 tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga
    11041 catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca
    11101 ggagaaataa aagaagcgat taatgaagct tttggtgatt tttcttcttt taaagaagaa
    11161 ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat
    11221 ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca
    11281 ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga
    11341 ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc
    11401 gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa
    11461 aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg
    11521 caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa
    11581 tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc
    11641 agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat
    11701 tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga
    11761 ataaagacac gacttcttag agagtcgtgt ctttttgtct atttatgaag tttttagatt
    11821 ttttggctaa gtaaatgcat ttgttcactt gacatgtcct aactaattat caagcaagga
    11881 acattttctt cgttagtaat ggattttaaa gaaatttcag tttttggatg ttgaataaaa
    11941 gaccatttgt gaaaccacta gtatacatac tcattaccat gtcaattcta ttactttaaa
    12001 agaaaatcgt tttactttga agaggagcat atctatccat taaaagatga tggtaatgca
    12061 atcactagtt cgagtcattt taagagccat gcagtgagct ggaaataaaa cgaatagaag
    12121 atgggtttaa caaaaggacc agtcatagga agaaattgtc gtgaaatcga atgataatct
    12181 gaacggtagg caaagactgt taaaaagatg ggaaaggtat gtgcgatcat tttactgcac
    12241 gattatttgg aaaatacctt gttgttcgct ttttctggaa aaaatacata aaaaaagata
    12301 aaacgattgc gaactgcgtg tattcggtct ttcgatcttt ttcacggtat ggtatgatag
    12361 ttaagaattt tgagaaattt taaaaaataa taatcgttac acaaaaatgt tttttaaagt
    12421 tttcttatga ttaaaaagtg aatttgctga ttgaacagtt atatgagaca atattttaga
    12481 aaatgtgaaa cagaggtgta ttatgaagcg atggcaaaaa gttctattaa ccttgttagg
    12541 ggtattcgtt ttgctgattg ccggattatc agcatacgga atcagattta tgggagaagc
    12601 gagccaaaca gtaaacaaaa tttctaaaaa ctctaatcga atatcctcca aacgttctga
    12661 gagagtaagc attgatgaca aagaaccttt ttcggttttg ctattaggtt tagataccgg
    12721 tggattagga agaacggaac aaggacgttc tgatacgatg atggtagtaa cagttaatcc
    12781 gaaacaaaaa aaatcaacga tcatcagttt ggaccgagat atttatacga atatcgtagg
    12841 ttatggaacg gtagataaat taaatcatgc ctacgcattt ggtggtgtcg aaatggccat
    12901 ggattcaatt gagcaactgc tcgatattcc tattgatcat tatgtaacga tcaatttgga
    12961 tggcatggaa gatttgatca atgcggtcgg tggcatcaaa gtgaataata aaattgactt
    13021 tactttggat ggtgtccatg taccagtagg tgaacaaaca ttgaatggag aaaaaggttt
    13081 agcctattct cgaatgcgac acgaagatcc agaaggcgat atcggtcgac aacgtcgtca
    13141 gcgggaagta gtgacgaaaa tcgtcaataa agttttaagt ttagatggcg taagcaatta
    13201 tagaaaaatt ttaaaagcag ttgagaacaa tgtgacgacg gacttagatt gggatgacat
    13261 gcttgatgta gcgacgaact acaccccggc gtttgaaaca atcaaacaag accagttgca
    13321 aggcgaagga caaatgatca atgatatcta ctatcaaatt ttaggaaaaa atgatcttct
    13381 ttcgatccaa aatgaactga agaaacaatt aaatataaaa acaagcgata cattacctaa
    13441 tttgaaaaat gagaatgctt caatgatgtt ctttgatgat tcggaagatg gcgattcgac
    13501 aaacggtgat ggatcgaata gtgaagggta caccgattat tcttcctatt cagaaaacac
    13561 atatgatcag taccaagatc cgaataccta ttatcagcaa acagaagata caagtcagta
    13621 ctatacacca agtcaagata ctcaaagtta tgatccttac gctggacagg aacaaaatgg
    13681 ttatggttat gaccagaata atgagtatgg aacaacaagt gggtactaga aaaaaatagt
    13741 ttggttaata aagagtgcca cctaaattag acaaaaaatc taacttaggt ggcacttttt
    13801 ctatgtgtta aattacagga cgtctctttt ta
//
LOCUS       DAZUKA010000075        13808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000075 DAZUKA010000000
VERSION     DAZUKA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.075"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..536
                     /locus_tag="ACG8PN_001763"
     CDS             <1..536
                     /locus_tag="ACG8PN_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4861905.1"
                     /translation="LRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQIYPELAK
                     MEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFVLKLYFV
                     KDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAHAINRET
                     EYANWLTDVLEESNRKK"
     gene            complement(689..1162)
                     /locus_tag="ACG8PN_001764"
     CDS             complement(689..1162)
                     /locus_tag="ACG8PN_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4861906.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1300..2772
                     /locus_tag="ACG8PN_001765"
     CDS             1300..2772
                     /locus_tag="ACG8PN_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4861907.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2769..3479
                     /locus_tag="ACG8PN_001766"
     CDS             2769..3479
                     /locus_tag="ACG8PN_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4861908.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            3831..4115
                     /locus_tag="ACG8PN_001767"
     CDS             3831..4115
                     /locus_tag="ACG8PN_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4861909.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(4432..4815)
                     /locus_tag="ACG8PN_001768"
     CDS             complement(4432..4815)
                     /locus_tag="ACG8PN_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4861910.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(4817..5401)
                     /locus_tag="ACG8PN_001769"
     CDS             complement(4817..5401)
                     /locus_tag="ACG8PN_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4861911.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(5448..6923)
                     /locus_tag="ACG8PN_001770"
     CDS             complement(5448..6923)
                     /locus_tag="ACG8PN_001770"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4861912.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7104..7415
                     /locus_tag="ACG8PN_001771"
     CDS             7104..7415
                     /locus_tag="ACG8PN_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861913.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(7530..9866)
                     /locus_tag="ACG8PN_001772"
     CDS             complement(7530..9866)
                     /locus_tag="ACG8PN_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4861914.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            10008..10676
                     /locus_tag="ACG8PN_001773"
     CDS             10008..10676
                     /locus_tag="ACG8PN_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4861915.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            10679..11722
                     /locus_tag="ACG8PN_001774"
     CDS             10679..11722
                     /locus_tag="ACG8PN_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4861916.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(11959..12840)
                     /locus_tag="ACG8PN_001775"
     CDS             complement(11959..12840)
                     /locus_tag="ACG8PN_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4861917.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            12999..13727
                     /locus_tag="ACG8PN_001776"
     CDS             12999..13727
                     /locus_tag="ACG8PN_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4861918.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
BASE COUNT         4174 a         2474 c         2793 g         4367 t
ORIGIN      
        1 cacttcgata tattttgtta gggttattaa gtaagaagaa gatgacagga tatgaattga
       61 atcaatcgtt caaaaatgaa ataggagaat tttggcaagc aaaacatagt cagatttatc
      121 ctgaacttgc aaaaatggaa gagcaaggaa tcattcagca tcaagtagag atcacaggtg
      181 aaaaattgga aaagaaagtt tatgaattaa cagaagaagg gaaggaacaa ttggcagagt
      241 ggattcatac acccaccaat gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt
      301 atttcgtcaa agacgtgaat gatccggcat tagtagaaat tattcagcag cagcgtgacc
      361 ttcatgaaga aaaacgactt catctattag agcgaaaaaa aacgatcttt cctactgaaa
      421 aagaaaaaca tgaaaattac ggacactacc tgattttagc gcatgcgatc aatcgggaaa
      481 cagaatatgc aaattggcta acagatgtat tagaagaaag taaccgaaaa aaataaaaaa
      541 atatccgaac agtgatggag aattcctttc tatatgaaag aaacgcagat caccgttcgg
      601 atattcttat gttcaaacag gccttttttc aagtatttat gtagcatcta cttattaaaa
      661 agcttggtac ttaggagatt agtggcgttt agagttcttt taacaactgt tcgtagtcag
      721 ctttttcatc gacaggaagg agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga
      781 tgtacatctt agcattctct tctttgaagt taccgatttg taaaatcgta tagcgttcgt
      841 cgatcaattc gatatctttg attgcttttg aaaaaacttt acggacatgg tgcattttct
      901 tttggcggat ttctgccttt tctgcagctt ttgacaaagt aaatccgtca tctaaatatt
      961 ttttgatcag ttctactttt atgactgtag gtaaacgata ttttctagga caattcgcat
     1021 cagcttcgat cgaacgaatg aatccctttt gttcccaata ccttaattga cgtggagaca
     1081 cacctacgat atcacttaat tcactgatgc caacaaccaa ttgatcattt tccaacaaac
     1141 gtttaaggga agccccaacc atttttgtca ctccttttgt cctcttgctt cccttaatta
     1201 tatccgtatt aacttatgtg tcaagtaggt tgacaaatag cgaatttagg actaaagtat
     1261 tctagtagtt tcagaaaaaa ttcagaaaga aggagaaata tgagtaacaa ccaacccgta
     1321 gatatttacg gaaaaccata taatcggaca cttttagttg ttgtcttatt aattggtaca
     1381 ttttgtaccg ttttgaatca gaccttattg acgacagctt ttcctacatt gatgaaagct
     1441 ttcgatattt cagcgtctga tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg
     1501 atcatgatcc caataacagc atggctgatc aataaattca gttcaagaaa tttatatctt
     1561 tctgccatga cgatcttttt gatcggaacg attacttgct ttacagcacc taattttagt
     1621 acattactaa tcggtcgttt gatccaagct tgcggtgtgg gagtatcgat gccattattg
     1681 caaaatatca tgctatctat tttcccgcca gaaaaaagag gatcagcaat ggggatgtca
     1741 gggatcgtta ttggacttgc tcctgcttta ggccctacat tatctggtta tatcattgat
     1801 aactaccatt ggcgtgactt gtttggtatg gtactgccaa tcgttgttct tgtcttggtt
     1861 ttagcattct tcctaatgaa aagtgttatt ccattgtcaa atcctagcat tgatatttta
     1921 tccgctattt tatcaacgat tggattcggt agtttacttt acggattttc tagtgtagga
     1981 gatgacggct ggggaagctt gaaagtaatt ggtttcttag ttgttggtgt gattttcatc
     2041 gccttgttct catggcgtca attacatttg gaacatccat tcttggaact tcgtgtattc
     2101 aaatcatcta cattcacgat tgcagctgtt ttagctggtg taacaaatat ggcgatggta
     2161 ggggcagaaa tggtcgtacc attgtatatt caaaatattc gtggagaatc agcattccat
     2221 tctggcttga tgttgttgcc gggtgcattg atcatggggg ctatgatgcc tgtgacgggt
     2281 gcgattttcg ataaatacgg cgcgaaacgt ttagccatca cagggatgtt cattttgaca
     2341 gcggcaactg caccatttgc tttcttgaca aaaacgactc cagttttata tatcgtgatc
     2401 ctttatgcga tccgtatgtt tgggatctcc atggtcatga tgccggtaac gacttctggt
     2461 atgaatgcat tgccaaataa cttgatcagt catgggacag cggtaaataa tacattccgt
     2521 caaatcgcaa gctcgattgg tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa
     2581 gcaaatatcc caagcaaatc tgtattggat aatacgccat tagcttataa agatcaggca
     2641 atcaatgcca cactgtcagg ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt
     2701 cttggcttta ttattgcctt tttattgaag aaaaaagaac ctgttctaat gaaagcgggg
     2761 gataatgcat gattattgga gttttgattg tcagcttgct tgcttttgct ttaacgaacg
     2821 tgttcgctaa aaaaacatgg caaacctttt tatctttgat tttcggacta atcttcgtcg
     2881 cttcactaag tttgatcgtc gctaatttgt ccaatcactt tggaatggaa aaagtaacgg
     2941 aaacaaaaac agaaaaaatt gtttccagtg ctgattccca aggagcagat atgcttttgt
     3001 ataaagcttt agggaatgga aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag
     3061 aaaaaccaaa agcaactggt acggataacg aaacaaataa agtagaaaag acagacggcg
     3121 atgctgaaaa agtgacgaaa actacttatt gggaatacaa aaacgacatg tataaattct
     3181 ggttcaatat tgctgataac aatcacgaat atgacagccg agtgaataca ttcaaaatcc
     3241 cagagacatg ggtagaatta agtacggacc aagcagccaa gttagctgaa ttagtgaaga
     3301 aacagcaaag tacgatggaa agtgaagcaa aagcttatgt tcaagacggt atggtaaaag
     3361 caatgactga aaatccaaaa atgagcaaag cagagcaaga acaacgtacg aaagccttag
     3421 ctgctgaatt ccaacaacaa gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat
     3481 attttgaaca ccatgtagga ggttgtgaaa aagctgctct gcatcaagca gtaagaacaa
     3541 tcagataaaa atagcactcc atatttttca tctgattggg cttagtgccg tagatgcaag
     3601 cttttgaaca ccgtttaagg aagaggttgt gagcctgccg tttaactctg agtcacaagg
     3661 agcaatttca aaaaacagct tctcatgttt gttgactatt gtgacttgtg accgaggagt
     3721 tggcaggaga acaccgttta ttcgaaaaaa ggaactgcga caagactttt gtcacagttc
     3781 cttctttttt ttgctatgct atactagatg taaaaaatag gagaaataaa atgagggaaa
     3841 tttattcttt tgaatcaaaa atctatgctt ccgaagtcgg caagggcggg gcatatatta
     3901 tttttccgta tgatataaga aaagaattcg gtgctggaag agttaaagtc caagcaactt
     3961 ttgacaatgt gccgtatgaa ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt
     4021 cagtctgcta catccttgga ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg
     4081 gtgatattgt agcagttaca gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc
     4141 acaacctctt gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
     4201 aaaaatgtgg aaacccatgt ttacaaaatt tttcttacta ctcgaaacga agcggcttta
     4261 tcacaacctc ttgataaacg gcgttcaatc agctgtccct cggtattagc tcaaaaacgg
     4321 caaaatatga gaagctattt ttccattttt ttctctcata ctcaggtcat aacgctgatt
     4381 tcacaacctc ttctttcata atggtttttc accatcttta taataaactg gttatttgat
     4441 atggaaatct attttttcca accaatctaa aatcttgtaa cctaactctt ctgacatcgg
     4501 aaaagaggcg ttgatttgtg cattggagat atggtcaaca tggataccgg aagtgatcgt
     4561 acaacttccc ggaacggcag ttttgatgat cacagctatt ttttcggcta agacatcgtc
     4621 tttatggaac cgaccatcgt ggctagggaa acgtacagtt tgcatctctg tatcacgaga
     4681 aaatgtagtg actgttccga tatgtggtgt atctccgcct gttaaagtga tcaataagtc
     4741 tttaccgatt actttaacag cagcttggat cgtgaaacct agtttggtta cttcaactat
     4801 cgtttctttt tccatattaa tcaccctgcc aacgttttga atgctcattt acaatttcaa
     4861 attgatccaa aagtttcatt gtatggtgat taacaagatc ttggatcgtt tgaggctgat
     4921 tataaaaagc aggaattggt ggtatgattt gcacacctag tcgtgctaat ttagtcaagt
     4981 tttccaagtg aatggcattt aatggcgttt cacgaggaac aatgactaac tttcgttgct
     5041 ccttcattgt tacatcagca gcacgtgcaa tcaggttgtc gcctaaacca acagagatac
     5101 ctgcgactgt tttcatactt gccggtacga tgatcattcc atcggtcaaa aatgatccgc
     5161 tggcgacagc agcacctaaa tctttgtttg aataatggta atcaaacagg ctttcgaatt
     5221 cagttagtga taagtcggat tccagcttta agttctgttt tgcccatgga ctgatgattc
     5281 cgtgtgtttc tatcatcgga tattctttta gtttcttcac taggttgatg gcataaatgg
     5341 tgcctgaagc gccacttaca ccaatcacga tttttttctt ccgttgtgcc aattgttcca
     5401 ttttccgcac tcctttttat gatgtcaaaa cagttgcaga tcattcatta tttcaaatat
     5461 ttttcccaat cttctacttc ttggaatttt gcacgtacaa aatgttcttt catatcgaaa
     5521 ggaactgtac cgtcaaagat agtttttgca cttatcccac ggaaacgaat agagccagga
     5581 ttatattctg gtaattctga tgggtccaat ggatggttcc tcattcctgg gatcacgatg
     5641 atatctttat ctccttggaa acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa
     5701 atatcaacat cttcatcgac taaaatgact gtcttcaatt ctttgaaggc tgataaagca
     5761 agaatagctg cttgacgctg aataccttca tccgcttcgt tttctttacg gatttgcatg
     5821 atggtcatca atttaccacc acctgcagga ggattgtaga catttactac ttttcctgga
     5881 atagctttat cgaccaaggc aagaatactt gcttctgtag gaataccggc catagatacg
     5941 tgttcttcac tgggaccaat cgttgtttgc atgattggat tgtcttttcg gtgagtaact
     6001 gctttgactt tcactacttg tacagctgga ttagcatccc cagtatagcc aggaaattct
     6061 ggcattgctt ttcctgagtt cgtgttgata tcttcttgca ttgtttcatt tggcatgatt
     6121 tctgcttcaa tgatgaattc agcacgagca atacccactt catcgactgc tataccatca
     6181 acgatttgaa taggttcgtt acgcaaagct cctgcacacc atagttcatt gtagccaagt
     6241 ggagtggtag gtggttcgaa agtcgtgcca atggcaattg ctggatctaa accaatattg
     6301 atagtgatag gcattggttt attcaaggct tcgaattgtt tttggaagtg accaatgtgg
     6361 cgtccgccag gcatgatgta cataccgatc gtatctttat cttctagaac catacgatgg
     6421 atcgttacat cgctcattgt tttttctgga tctgatccta agactacacc cattgtgatg
     6481 tatggaccag catcttcagg agtatttgta ggtgcaggca aaatgttgcg aatatcgaag
     6541 tctgcatcgg tagctagatg aacgacttct tgtgctggag cgtctgcttt gtctactttc
     6601 acaggtttga ttggattttc tacagcgtct ttcaagaaac gaccaagtgt tcgataatca
     6661 tggtgcaaaa tttcacctac acgtttacga ctagccattg ttccgatgtt cacacgtgtg
     6721 ccggggaaac ctttgatatt gttgaaagtc atagctggtc cttcttgagt cggacgtttt
     6781 actgtaccgc cagcaccgat ataacggtaa acaccagata attctgcttc aggatcgatt
     6841 tcaatatctg tttcgtgata ttgacctgga agtgttttta gttcttcgat gacttttctt
     6901 aaatcatacg gttgatctgt catgagagat tcctccttag ttgtctttac ctatctatag
     6961 taaaaggtga aaaagaggaa aacaattcaa cttgttcggt aaagtattct acaatggaat
     7021 ggatgcgttt ttttgataat agaataaact atgatatact tatcactaaa agaaagcgca
     7081 tacttagtgt tggaggaagg aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa
     7141 aagtggaaaa tatagcgaat attttgaaag ttacatttga aaacggagaa gtaaaatacg
     7201 tgaaatctca ttggacagaa gagataaccg atgccttgca atttggaaaa aaaggaagag
     7261 gaaaaagaaa aaatcttctc gcactttctc gtaatatgtg gattgggact gaagtaacga
     7321 ttgaagcaga tggaacagta ttcataaatg gaaaagacag gtatacgcca gaagagttgt
     7381 ggtacaaagg aaagaaaagt ataccagaat tataaaaaat aattaaaaaa aacagcgcga
     7441 aacaaggaac atattacttc ctcgtttctt gctgttttct actatttcag cgctcttatg
     7501 actcaaatct aaatgtggtt gtcacaacct tattctgcac gcaaagcgat agcggcgtct
     7561 tttttcgctg ccattcgtgc aggaagatga ccgccaagca tcgtcaatat agtagagacg
     7621 atcactagaa taatcgcatg gacaggattt agttgagcaa cattttttag atccgtcatg
     7681 ttgtataaga tgctattgat agggaacgta gctagccatg caataaacac acctaatatc
     7741 ccagaagaaa tacctaaaat acacgtttct gcgtcaaata cgcgagtgat gtctttttta
     7801 cgtgcaccta aagcttttag tacaccgatt tcttttgtcc gttcaatcac agatgtataa
     7861 gtaatgatac tgatcatgat catacttgtt actaaagaga tccctgcaaa agcaataagg
     7921 acataagtga tagcatccat caatccgcca gtcagttcag tcatcgttcc agctaaatca
     7981 gtataaatga ttttatcttc atctgatttg cctttattgt aatcatctag ataatccaag
     8041 atcttttctt tgtcttcaaa attgttcgga taaatcatga tacttgaagg caagctgtct
     8101 ccacctaaat aactaagcaa tgtttgtttg gcattttcgt caactttttc agtagttaag
     8161 acatttacac cactgtctct ttgagctttg acgatttctg attctttgtt ttcgttgacg
     8221 atttgagtcg ttaattgatc gctataagcg attcctggag ataagagatt cattgtagaa
     8281 gaagatttca cgcgtaggat ccccgagatc ttcaattcat cagaagcatt ctgatacatc
     8341 gcatcataat ctgtattagg gataaaattc ccagttggaa gttttgtata gtaagtattg
     8401 ttgtttacta atttgaatgt tgtaccaacg atatcatcaa aatcaagttt ttcgtcttct
     8461 ttcacatcaa atccaagatt cttcaatgcg ttgatattag tattattatt gccgtctacg
     8521 atcagcacga catctgttgc tgaggctgga taacttccgg caagtaaaga ataattgtct
     8581 tttaaaaagt tttctttgct agtgtcaagc tgtgtaggga aacttgacac tcccacacct
     8641 gtcattgctg acatcgtact agataaagaa agagattccg tatcaggatt ttgattcgag
     8701 aagctgacag gctgtacttt gccatttaca tcacgtaaaa gattgattcc tgtagtacgg
     8761 gtaaatccga tattattact taagttagga tcgatgtctg ttacgtaatc gacgtatgtt
     8821 tgatcaatct tattcgtgtg ctgtgcttta tcttcttcgc tgacttttgc agtaatcgtt
     8881 ttgctgtctg gataatccgc tttgcttgca cccaatccgg catcgtcacg agtctggtta
     8941 gtcgttacct tagaaatagt aatcggaaat ttcgccatcg tttcagcttg cgtgttatcg
     9001 atttgttttt ggaatccaga agaaagggac agaacgatcg cgattccgat gatcccaata
     9061 ctggaagcaa aggaggtcaa gaaagtacgt cctttttttg ttcggatatt gttgaaggaa
     9121 agctttaacg ctgtccaaaa actcatcttt gttctgcgta agttaaattg atcatctttt
     9181 ggtcgttcga tgtgaggatg ggaatctgtt aagatcttac catcagaaaa ttcaatgatt
     9241 cgatctgcgt attgatgggc cagttcagga ttatgtgtga ccatgatcac taatttttct
     9301 ttcgataact cttggatcaa ttccatgatc tggatacttg tttctgtatc taacgctccg
     9361 gttggttcat cgcacaaaag gatatctggg tcattggcta gtgctcgagc gatggccact
     9421 cgctgcattt gtccaccaga taactgattt ggctttttat gcatatgatt tgtcaagccg
     9481 actcggtgca atgcatcttc tgcccgtttt cgtttttcgt ctttagaaac accacttaat
     9541 gtcatcccaa gttcaacgtt ttcaataatg cctagatgac caatcaagtt gtaactttga
     9601 aaaatgaaac cgatagagtt gtttcggtag gcatcccagt cactgtcctt aaagtcttta
     9661 gtagactttc cgttgattac catatcaccg gaatcataat tatctagtcc accaatcaca
     9721 ttcagcatag tcgttttacc agaaccacta ggacctaaaa tcgctacgaa ttctttttgt
     9781 ctgaaagcga cagacacacc atccaatgct ttggtggtgg tttcgccgac tttataatat
     9841 ttttttatat cttttaattg aagcataatc acccttctcc cgtattgaaa tattcggtat
     9901 actaataagt atatcagaac aatgatcgta gtatacgaaa taaatgttaa ctgaagatga
     9961 actacaagaa aaaatctaga aatttttcaa aaaacagagg gagatcaatg aatcgtattt
    10021 tagtagtaga agatgatccg caattacagt tgctttaccg ttctgtgttg gaacgagcgg
    10081 gcttttcagt catcaccgca ttgaatggag aggatgcgct gaaaaaacta gaagcgacac
    10141 gtatagaatt aatcattaca gatatcatga tgccgaaaat ggatggctat gacttgctag
    10201 actcattacg tagcaccaga tttgacacac ctgttctaat gattacggca aaggccgatt
    10261 ttgaggataa gaaacgcggg ttcaaattag gagcagatga ttatatgaca aaacccatcg
    10321 atgtcaatga gatgatccta cgtgtggaag cattgcttcg acgttcaaag atcaattaca
    10381 gcagtagctt gataatcgga caaacaaaat tagagctaga tgcttataca gtgttacaag
    10441 atcagcattc gttgattctg cctcaaaaag aatttctgct tctgtataaa ttactgtctt
    10501 atcccaacaa gatcttcact cgccaacagt tgatggatga tatttgggga ttagatacga
    10561 atacagaaga acgaacgatc gatgtacata tcaaacgttt aaggacccgt tttgaaaaga
    10621 cggaagattt tcaaatcatc acagtacggg gactaggcta taaggcggtt atttaaagat
    10681 gaaaaaaaag ctattttcac aattatggat ctattttgct agtattgtct tcatttcgat
    10741 cttagtgaca ctgctgtgtt ttctcggttt tattttctta ttgtctcgac attctatctc
    10801 gcctgctgaa tcggctggaa aaccaacgtt atacccgtta ttcgtctttg cggggttcag
    10861 tatggtcgtg ggtacaggaa tatcgatttt tgttgggcgg cgtatcctgc atccaatcag
    10921 tgcgctaggg acaaatatga gtctggtagc aacgggagat ttttcgattc gaatggatga
    10981 acagcagaaa gtagcagaag tacagcaact gtataaagat ttcaacgtta tggtccaaga
    11041 actcaatagt atcgagacac tgaggaatga ctttgtgtcc agtgtatccc atgaattcaa
    11101 aacaccacta gcaacaatcc aaggatatgt tcaattgtta caagccccaa atctctctga
    11161 cgaagaacgc caaatatttt tatatcggat tatcgaaagt atcacgcagc tttcacaatt
    11221 aacagagaac atcttaaaat tgaacaaact ggaaaaccag cgtatccaac tggaaaagaa
    11281 agagtatcga ttagatgaac aaatcagaga agtaatcgtt ttttttcagc ctaaatggga
    11341 gaaagaacag ctagaattgg atatcgaact tgctgcagta aattacacag gcaatgaaga
    11401 gtttttgtac caagtgtggc tgaatatcat ggataacgcc ataaagtaca atcaaatcaa
    11461 tggacagatt catatcaaac tatttgagac agccacagag attgtactgg aagtgaccga
    11521 ttcaggtgta ggaatgaatg aagagaccag agatcgaatg tttgaaaaat tttatcaagg
    11581 agatacaagt cgtcaaattt ctgggaatgg gctaggactt tcactggtta aaaaaattct
    11641 agaactgcat gatggacgta ttgattatag cagtatcgaa ggagtaggaa cgacagcaat
    11701 gatacggtta agtaaacaat aggaaaaaga ataaaacccc taaaaattct attgaaggtt
    11761 tttaggggtt tttagtcgtt tttttgtgat tagggaaatg ttcttacggt tcttttggca
    11821 taaaaatcgc tgctttctta ttgaactacg agaaatgtta gaggtgtcat tgaaacaagg
    11881 acatgatgca agaaagagag cgaaagtcat gcgtcgctct ctttttttgg gtggatagat
    11941 ggacagaaaa tgtagggctt agttttcttc ttgatttaaa tactcccaaa atgtatctga
    12001 agccaaagaa tgctcaccga ctttacgagt aaatgcaaga taactctctg ttgttggaga
    12061 aatagggaga atcacagtat tttcttttaa tagattcttg actgtttttt ccataacaat
    12121 agcaatcccc atgtcatttg caatcatatt gatgatcaag tcgattcttg ctcctttata
    12181 agagattttt ggttcaaatc cagcttcgcg acacagatct agtacaggtt gatacaagtt
    12241 cgtagtgggg ccaaggagca aaaagttctc ctttttcagt tcgcttagat ccaatttctt
    12301 tttactagct aaaggatgat ttttaggtag aactgctaca aaatcgtctg tttcagtagg
    12361 caaccactca atcgaacttg gtggggcctg gaaacttcta gcaaagtgga tcgtgtcact
    12421 atgttcattg gcgtcattgt aagttaattc gacactttcc acttctttca actggactgt
    12481 aaattcagga tggcgtttca aaaactcggt gattttggaa aaacctttat aattgatcat
    12541 tgtcggtatc gttaagatat ttaatgaaag attgttgtct ttcgtatagg tttcaagcac
    12601 ttcttgcata gcatggtatt gttctacgac attttttaca tatgggagca ataagcttcc
    12661 agcttcggtc aattcgattt ttctatgggc gcgttcgaat aatttgacgt ctaattctgt
    12721 ttctaacgaa agaatctgtt tagaaatatt tccttgggtg gtgaattgtc gatcagcagt
    12781 tttggtgaaa ttcattgttt cggctaaatc aagaaaaact tcgaatttat ggatatccat
    12841 catattcctc ctacttattc caataaagaa tactttaaca caaaaaatga attggtgtaa
    12901 tcttctctgt ctgttacaat ctttcttgca gcgaaagcaa taattacaga aatttttctg
    12961 atttttttcg cttcgtagaa aaagagagga gttttttgat gccaactgga gttttgatca
    13021 atgtaggttc cgttttgttt ggcggactaa ttggcggatt agtaggaaat aaattaagtg
    13081 aacattttaa agcacagctg acgatggttt ttggtgtgtg ttcgatgggg atgggaatct
    13141 attccattgc gccgatgaaa aatatgccgg ctgttatttt tgctttagtg ataggaacag
    13201 cgattggttt gattgttcat ttaggtaatg ggataaataa gggggctgcg ctgatgcaag
    13261 tacctatttc taaaatcttc cctagtgaaa agcttggtat gacccacgat gaatttatca
    13321 gcacattagt gacggtcatt gttctatttt gtgcaagtgg tacggggatc tatggaagtt
    13381 tagattctgg gatgacagga gacagtacga ttttgatttc taaatctgtc cttgattttt
    13441 tcacagcagc tatttttgcg tgtaatttag ggtatgtcgt ttctgtgata gcaattcccc
    13501 aattcattat tttttatatt ttgttcttat tggcaaaatt tatttatcca ttgactacac
    13561 cagacatgat cttagatttt aaagcttgcg gcggattctt gatggtcgct accggtttta
    13621 gaatgatcaa cgtgaaaatg ttcccagtag ctgacatgat acctgcaatg atcgttatca
    13681 tgccactgag ctggatgtgg acaaattgga ttatgccact gttataataa aaatcaaaga
    13741 gaagccattt tttgctgact aaaaaggatc aaatctttta gtcagttttt ttgtatttga
    13801 caaactga
//
LOCUS       DAZUKA010000076        13745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000076 DAZUKA010000000
VERSION     DAZUKA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.076"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2068)
                     /gene="glyS"
                     /locus_tag="ACG8PN_001777"
     CDS             complement(<1..2068)
                     /gene="glyS"
                     /locus_tag="ACG8PN_001777"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4861919.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQII"
     gene            complement(2070..2987)
                     /gene="glyQ"
                     /locus_tag="ACG8PN_001778"
     CDS             complement(2070..2987)
                     /gene="glyQ"
                     /locus_tag="ACG8PN_001778"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4861920.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3371..4183)
                     /gene="recO"
                     /locus_tag="ACG8PN_001779"
     CDS             complement(3371..4183)
                     /gene="recO"
                     /locus_tag="ACG8PN_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4861921.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4326..5225)
                     /gene="era"
                     /locus_tag="ACG8PN_001780"
     CDS             complement(4326..5225)
                     /gene="era"
                     /locus_tag="ACG8PN_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4861922.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5240..5635)
                     /locus_tag="ACG8PN_001781"
     CDS             complement(5240..5635)
                     /locus_tag="ACG8PN_001781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4861923.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5619..6095)
                     /gene="ybeY"
                     /locus_tag="ACG8PN_001782"
     CDS             complement(5619..6095)
                     /gene="ybeY"
                     /locus_tag="ACG8PN_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4861924.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6112..8304)
                     /locus_tag="ACG8PN_001783"
     CDS             complement(6112..8304)
                     /locus_tag="ACG8PN_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4861925.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8328..9299)
                     /locus_tag="ACG8PN_001784"
     CDS             complement(8328..9299)
                     /locus_tag="ACG8PN_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4861926.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10005..11384
                     /gene="brnQ"
                     /locus_tag="ACG8PN_001785"
     CDS             10005..11384
                     /gene="brnQ"
                     /locus_tag="ACG8PN_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4861927.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11538..11984)
                     /locus_tag="ACG8PN_001786"
     CDS             complement(11538..11984)
                     /locus_tag="ACG8PN_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4861928.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12011..12187)
                     /gene="rpsU"
                     /locus_tag="ACG8PN_001787"
     CDS             complement(12011..12187)
                     /gene="rpsU"
                     /locus_tag="ACG8PN_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4861929.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12349..12777)
                     /locus_tag="ACG8PN_001788"
     CDS             complement(12349..12777)
                     /locus_tag="ACG8PN_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4861930.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12869..13729
                     /locus_tag="ACG8PN_001789"
     CDS             12869..13729
                     /locus_tag="ACG8PN_001789"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4861931.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3757 a         2866 c         2485 g         4637 t
ORIGIN      
        1 tgatgatttg atctaaactt gctagtgaca aagccatggc agcaatttga cttaattgcg
       61 ttaatcggtt cttacgtact gctggttctt ctgccatgac catcgtctga tcaaaataat
      121 tttcgatcag tgggcgaagg cttacaagct gatcataatt ttcgttgatc gtttggtcag
      181 caaagcttgt tttcattgcc tctactgctt tgtgcagttc tttctccgct tcgttttcga
      241 acagttcagg gtctacttct gattgttttt caagctccgc ttttttcgct aagttcacga
      301 cacgtgtcaa agcttccatc gatggtttga agtcttcatc tgcaagatgc tgcttgaaca
      361 attgtgctgc tgcaaatact tttgtcagat ctttttgttc tgcattcaaa acagcttcga
      421 tgacatcatg acggatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt
      481 cgatcacctg ttgttgtccc gctgacaagc caatgccaaa acgatccgta tctttactga
      541 taacttcact aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga
      601 tgattcgaac gacaccatat gcttgacggc gcaaagcata tggatcgttt gagccacttg
      661 ggatcatccc aactgcaaag aaagaaaaga tactatctaa tttatcagca atcgctaaaa
      721 cagaacctac tgtagtctca ggtaaatctc cttcactgga tataggcatg taatgttcac
      781 ggatcgctgt tgctacggct ggtttctcgc ctttaagcag agcatatttt tcgcccatga
      841 tcccttgtag ttcagggaat tctcctacca tgttggttac tagatcaaat ttatagattt
      901 cagcagcgcg ttgcagatcc atcaattctt cctcagatag tcctacttgt tcgcctatga
      961 tgcgagcgat gacttgtacc cgttgcattt tctcatagat gctaccgatt ttttcatgga
     1021 aagtaacagt ttttaatttt tcaacgtatt ggtcgattga agctttcttg tcttcattat
     1081 aaaagaattc cgcatcttct aaacgagcag tcaatacttt ttgattgcct cgtaccacat
     1141 tttccaattt tacccgatcg ccattacgag cagaaataaa atgaggaagc aacatacctt
     1201 gatcgttccg tacttcgaaa taacgttgat gttctttcat agaagtgact agtacttctt
     1261 caggaacaga tagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta
     1321 aattgttgac ttcttctagt aaatcttcat ctagatcaat gatccagtga tttttttctg
     1381 ctaattcgtt tgcttgtgtg atgatcattt cttttctttt ttgcggtgaa gcaataacaa
     1441 attgttcatt caatttttct tcgtattgtt ttgcatgcgt aaatgtcaca tcttcgccta
     1501 agaaacgatg acctcttgaa gctctgtctg ttttgacatc caagatctca aacgggatta
     1561 tttcgtcatc taacaatgcc acgatccagt gaatagggcg aatatattca aaatcgtatt
     1621 ctgcccagtg catcgttact gggaaagtga ggcttttaat gacatctgct aaacctgtca
     1681 atacttcttt tgcttctttg ccgtgagtgt atttcgttac atagacatat tccacgcctt
     1741 ttaattctct aaaagtgata gcatctgtcg tcagaccttg tccgcggaca aatccttcag
     1801 ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt
     1861 cttcttcgct gtctgcttga cgttctggaa tgtttgttac tttgattgct aagcggcgcg
     1921 gtgtagaaaa cgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg
     1981 ttttttcttc taattgtttc atgcttggtg tgactacatg agcaggtatt tcttccagcc
     2041 cgatttcaag taataagtct tttgccatct tattcagcct ccttcacgtt ttctggattt
     2101 gctgtgtttt tattcaataa agggaatcct aatttttcgc gttctgccac aaagattttg
     2161 gctacagcac gagccatgtt gcggatacgg gaaagataac ctgcacgctc tgttacggat
     2221 actgcaccgc gagcatctaa taaattgaat gtatggctac atttcaaaat ataatcatac
     2281 gctggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaaa
     2341 ttgcttagta gcaggtcttg gtcgcttact tcaaaagaat atttagaatg ttcatattct
     2401 ggttgtttga agatctcacc atatttcacg ccatcagccc attccagatc ataaacactt
     2461 tcaacttctt ggatataaga agcaagacgt tctagtccat aagtgatctc agaagtaact
     2521 ggtttacaag gtagtcctcc gacttgctgg aagtatgtga attgtgtgat ctccatccca
     2581 tcaagccata cttcccagcc cacaccggca caacccatcg atgggttctc ccagttgtct
     2641 tcaacaaagc gaatatcgtg ttctaatgga tcaattccta aaagacgcaa gctttctaaa
     2701 taaagctctt ggatattttc aggtgaaggt ttcattacga cttgaaattg atgatgctga
     2761 tacaaacggt ttgggttttc tccataacgc ccgtcagctg gtcggcggga tggctctaca
     2821 taagcagcat tccatggttc cggtccgatt gcccgtaaga atgtatatgg gctcatcgta
     2881 cctgctcctt tttctgtatc ataagcttgc atcagcatgc agccgtttga tgaccaaaat
     2941 ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct
     3001 ttcaattttt aagcgttgct gtcaataaga atacaggaaa gaaaacgaca aaaagtccct
     3061 atgtctgatt acagacatag ggacgaataa tttcgcggtt ccaccctact tccgaataaa
     3121 gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg
     3181 cttccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt
     3241 attcattttg cagttttctg gtttgtataa tagatcctct cagtgaacga tctatttaat
     3301 ctacggttaa taatagacgg aagtccctgt atttgtcaaa tagttttgat ttttttgttg
     3361 tatctttcat ttattcttct ttttcaggta tctctttttc aggcatattt ttctctggcg
     3421 tctttaatac gtgtgcccaa gacttcattt gatcaatgaa ctttttgctt tttagatgaa
     3481 taccgacata ttcatcatac aactggtcaa tgaggtcacg gattgcctgc ttcgtttctt
     3541 ctttcaagtg gattccactg atcttatcat aagaaatagc cgaaaacatt cgaacgaaat
     3601 aaacagcgcg aggatctgca tgataacggt gttcgtccat tggccagtgt ttctcgcata
     3661 tcacaccgcc atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacac
     3721 agtgcgtcca aattggcgcg attccgaagc gttgcagtat ttgcacttca aagatattcg
     3781 tgatgatttc tgcatcatct ccttggtcca tccgctgcag agcttgttgc gtaaattgga
     3841 ataaatgcgg gtcatattga tgatcttcga ttgctgcatc tactagattt agcaaatacg
     3901 taccataacc gctcaaaaag atatcttctt ggatgttccg aaacgcatgg acgtctttga
     3961 cactgcttaa aaacgacaaa ccttcacttc gaaaattacc gatatagact gcttcggtaa
     4021 aggggagcaa agccggagct aatggctgat tttgccgatg gattcctttg atataaaaca
     4081 tcattttgcc agaagattcc gtaaagattt tgactaattt atctttttct ttgtaattac
     4141 ggctagataa aatcaagcct ttcgattctg caagctggct catattctcc ctcctaaaaa
     4201 ataaatctgg aaaagcagat tctgccgatc cagattattt atgtttcgat cttccgctgt
     4261 ttttctcaac gtacggattt ttatccagtt tatttctata cctaacctat aagaagtcca
     4321 gaatatcagt actcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc
     4381 cagtcttttt ggactttgac ccaaagttct agaaatactt tgtctcctaa aagattttcg
     4441 atatcctttc ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg
     4501 attccttttt ggctatctct ttcgacgatg atcgtggcct ggatatggat tttatcattc
     4561 tcgtttcgct tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt
     4621 aaaaacaaga ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc
     4681 acttgatcat ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatt
     4741 aagcgttcga cattgtttcc ttctgtagca gagatcggaa tcacttgtgc aaaaggcatc
     4801 tgcgtcgaat aatcttcgat gattcccagc aactcgtctg gatggatctt atcgatttta
     4861 ttgataatca gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct
     4921 ccccggccac gtttttgatc agcactgatc ataaataaga ctgcatccac ttctttcaac
     4981 gcactgtaag ctgcttctac cataaaatct cctagacgat gctttggttt atggattcct
     5041 ggtgtatcga taaaaatcag ctgagcttct ggtgtagtat atactccttg gattttattt
     5101 ctagttgtct gtgccttgtc actcataatg gcaatttttt gacccacgat tcggttcaat
     5161 aatgtggatt ttcctacatt cgggcggccg acgattgcga caaaccctga tttatgttct
     5221 ggcatatacg ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa
     5281 tcaacccaat gatgacagca aaacaagcgg tcactaaaac tgctccagct gccatatctt
     5341 tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa catgtccact acattttcaa
     5401 aaacagtatt gatgatctct actatccaaa cgagaaaaac agctaaaagc agccataacc
     5461 actctatccg atcaagctgg aaaatcacgc ccataaataa agcaaggata ccgaacgcaa
     5521 catgtttcct catattgcgt tcttcctcaa aaacggtttt gactccttgg acagcaaatt
     5581 ccacagacat caggaaatgt ttgtttttct ctacgttttt atcttttgag tccataggca
     5641 tccaagatct ctttctgtaa accaaacatt tctttctcat cttctggtgt catatggtca
     5701 tacccgttga tatgcaagaa tccatgtacc gctaaaaagc caagttcccg atcatatgaa
     5761 tggccgtaag cttctgcttg ttcagcagca cgttcaatag agatcatgat gtctcctagt
     5821 acacgaggta atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccttct
     5881 tcttccagag caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg
     5941 attacttgga ttgccgcatt gtccatgaaa gtaacagaca tttctgtttc ctctggcagc
     6001 tttaaataag atgctgcaaa ttgcagcaag tcatcgattt ctttcatttt ctcttctgag
     6061 acttggtttg tttcatccat aaaggtaata tccattatac atcgcttctt tctattcttc
     6121 ttgttcttcc tttatcttct caacttctgt tttcatttta ggatatttga tccgagaatg
     6181 gaaggtgcta cttaaaccgc tgattaatga tttttccact tttcggattt ctttcaaggt
     6241 cagtccacta tcgtctaatt gaccatcgga aatacgctct tcgataagat tatggacaaa
     6301 actttcgatt ttttcgctcg tcggatgatc cattgcccgt actgcagcct cacaactgtc
     6361 agcaatattg acgatccctg cttcacgact ttgcggtttt ggtccaggat aacggaattg
     6421 ctcttctgtt acttccggat tgcgttcttt cgctttgatg tagaaataac gcattagcgt
     6481 ggttccatga tgctgtttgc aaatatcgat caccatttgc ggcatattat aatcttccaa
     6541 tattttggct ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa
     6601 gaaattgtgc gggttttccg ctcctgaagg caagttttca acgaaaaagc tagcatgttt
     6661 gatcttgccg atatcatgat aatagcaagc tactcgagtt agcaaagaac gtccgccgat
     6721 ttctgctaca gcgttagcac ttagattagc taccatcata ctatgatgat aagtacctgg
     6781 cgcttcttcc agcaactgtt tcaataaagg atgatttgga ttgctgagtt catttaatac
     6841 aattacactg tcatcattga ccatcaattc aatatatggg tgtagaccga tcgtcagcaa
     6901 gaatgagaag agtgcaccag tgaacccgca gacgagcatt aaccaagtcg ttgcatcacc
     6961 aaagctcatt ccttgataga cgatcaaaat gacatccatc atcacaggaa aaataatgac
     7021 ccacatggca gcagataatc cttgttctga gatccgtttg cgtctgacaa tcgttgccat
     7081 gagcccagag aaaacataag acatcaagat aatcgttaga aagttcgtgc caatcgaatc
     7141 ataaaaaatg aaaagtgcgg atactgcttg gaaaatacca gccatgatcc ctgctcttcg
     7201 attcaagaaa aagcttaaaa caagtggtgc aaaagcagcg ggataaaaca gcgggatata
     7261 agtagcttgt tccgtttgga ataattggaa aaatttcatc agcactatgc tgactgacat
     7321 agctgtcaca tagaaaagca cgtattttgt tctttctcct gcattttgga attgaagtga
     7381 attataaatc agtacgccga tttgaagcaa aacagctaaa accatggcaa ttagcggaaa
     7441 aatcgaagtt gtttgattag tcagtcctag taattccaat tttttcatag ctgaagcatc
     7501 gatttgatta ccttcacgaa caatgatttc tccttggaag atcatcactg gttgtacagc
     7561 ttctcgtgcg gattgtttca attcttctgt ctttttctca ttcaatacgt cattcactac
     7621 gataccttga tccactaagt agcgaatcat ttgttgttgt gctggtgtga cattcaaata
     7681 ttggatttgc tcttctgctt cttgtttaaa agcttccaat tcactttcac gaacttgttt
     7741 gctcatctgt tcgtcaatga gcttcagact ctcatccctc acagtatgaa tctcagtttc
     7801 agtcatttca aatatcgtcg aataaaaatt gttcggtagt ttttgataaa acacaacatt
     7861 ttctgcattc acattttcaa aatttttctt cagcgcggcg atccgctctt ctgtagtagg
     7921 ctttgggata ttttcatctt cctttgcctt atcaatcttt tcgtcataag atttgttgat
     7981 ggcatcattc gtcttatcga tcaattcaaa tagttgcttg atccgattgt tctggtcatc
     8041 tgcaagatct ttttggtaag tgtattctgg agtgactgct tctgctgcca gttttctttt
     8101 ttgttcggtt tcttccgtgt tctcgatcgt tttgttcgca cggatacttt cttctgctac
     8161 ttggccttct ctgaaatcat tggccttttg tttgacacta ctaaatgtaa cgccgcagat
     8221 aatgataaaa aacaagaaca gcaggaaagg caatgctgcc tttccaagtt tttcttgtag
     8281 tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatgg
     8341 agatctgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca
     8401 cttgcttcaa aattaacaaa atcgattttc ttgatttgct ttaatgttcg ttctgcatga
     8461 acaagaccac ttgtcgtccc tcgaggcaag tctatctgac ttgtatcccc attgacaatc
     8521 atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt
     8581 tgtgcttcgt ccaagataac gaatgcatca tccaatgttc gcccccgcat gtaagctaaa
     8641 ggtgcgattt cgattacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt
     8701 tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt tagatccccg
     8761 ggaagaaagc ctagattttc acctgcttcc acagcaggcc gcgtcaaaat gattttttgc
     8821 acttcacctt ttttcaaggc agcgattgct aatactactg caagaaaagt ttttcctgta
     8881 ccggctggtc ctacgccaaa tacgatatca tgtttggcta ccgattcgac atatttcttt
     8941 tggccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca
     9001 tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca
     9061 tcgggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag
     9121 gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg
     9181 atggttgttt ccgttgtttc ctcaatcagt tttatatgct tgtcgtgtgc acctaaaagc
     9241 atgctgacat catcagcagc cgtcaatcta atctctaaag aactagactc ttccgtcaaa
     9301 aatagacatc cccttttttt cgattcattc tttcctattt taccatactc agaagaaact
     9361 atatagcaaa taagaaaagg ttctaaaaga gttgcttaac cataagtacc aagaaacgtt
     9421 aaaaaactag ccgcttccat tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt
     9481 aagctatgca taagatagag gacaagtatt ccgtctaact tagcaagtat tctctgggta
     9541 tttttcttgt ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa
     9601 acaggtgtat tataacgtca acaaataaat atacagcaaa gcgtcgagga aaacagtagg
     9661 ctattcagtg atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag
     9721 acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc
     9781 aggctagcgg ataactttat cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa
     9841 aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg gcttttttta
     9901 tattttaagg tggtggtttt catggaatct gatattagtg gtatttctag tttctgcaac
     9961 gtttattttg ttactcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa
    10021 cttattcatg gaagcaatta ttgatggtca gttcacttat cttcgggatg ttcttcgggt
    10081 caggtaatct gatctttcct gtacacttag gtcaaatggc tggatcaaac tggatcagcg
    10141 ctggtgtagg atttgccatc tcaggcgctc ttttcccatt attagctatc ttagctgtag
    10201 tagtgacaaa aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg
    10261 ccttgttctt gatccttgtt catttaacga tcggaccatt ctttggtaca ccgcggacag
    10321 cagcaactgc ttttgaaatg gctgctaaac cctttttgcc tgaaaaatat gattcattag
    10381 gtatgttttt attctctgct cttttctttt tacttgctta tttccttact gtacatccaa
    10441 cacgtttggt caaatatgtc ggaaagtatt taaactcctt gtttcttgca ttattagcta
    10501 tcatttttgt cgtttcttta ttccaaccca tggggaatct aaaccaatct gctgcagatt
    10561 cttatcagac gaacgctttg acaaatggcg ttttagaagg atacaacacg gttgatgcag
    10621 tagctttact tgctttaagt gtcacttttg tccatgcagt tcgtgatctt ggttttaaag
    10681 ataaaaaagt cacagaatta acagcaaaag ctggtagcct gagcgttgcg ttagaagtca
    10741 tgatttattt tggtctggtc cttctcggtg cgatgagttt gaataagatg tctttatcag
    10801 aaaatggcgg aactgcactt tctcagatca ctaattttta tttaggtaac ttcggtgcta
    10861 gcttcttagg ggtaatggtc acattaggtg tcttcactac tgctatggga ctcgttgttt
    10921 cttttgcaca agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt
    10981 taacgacttt tgtctctttt gtcgttgcga atgccggatt ggataatatc atccaatggt
    11041 ctttaccagt attgatgctt ttgtatccat tgtcacttgc gttaatctta ctttccttaa
    11101 cagcaaaatt cttccaaaag agtccatttg tctatcaagt aacgatgttc ttttctgccg
    11161 ttccagccgt attggatatg ttggcaaatt cgcctgcatt agtgagtcag caaactgtcg
    11221 tagcaagctt actaggattt tatcatcatt atgttccttt tgctgcatta ggtcttggct
    11281 gggtcgtccc aacgttgctt ggttacgctg gcagcttgct cttttattat gtatatcgcg
    11341 tatcaggata taaacaagaa gcaagtgagt taccagaaga ataaccaaat caaacacaat
    11401 ttaacataag agatattaaa caggaaaaaa acagctagaa gaaagaataa gattctttct
    11461 tctagctgtt ttttagttct ttttttacga acacaaaaaa ctgcggataa tcactgtccg
    11521 cagtttaaat gataaaatta agattgtaat agttctttca caatcgcatt cacttggttt
    11581 ccatctgctt ttcctttgac tttaggcata attgcaccca taactttgcc aaattctttc
    11641 attgatgaag caccagtttg agtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg
    11701 gaaagttgtt ccggcatata tttttcgact attacaattt cactttttac tttttcagct
    11761 aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttca
    11821 cgagacaaca ccgttagttc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg
    11881 ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc
    11941 atcgctgctt tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt
    12001 catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta
    12061 cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca
    12121 cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt
    12181 ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat
    12241 atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg accgtcaagg
    12301 aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt
    12361 ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtgcatccac ctagctgttc
    12421 ttcgaaataa ttcatcggac acatatggat ttctttcgtt gttccgcaca ctgtacagat
    12481 aaaatgatgg tgatggccga ttttcccctg acagcaatga aaacgaaact tcatttctcc
    12541 atttaaatcg gtttcttcca gtaaatcgat ctctgcaaaa tcatggagat tccggtaaat
    12601 cgtatcataa cttacccctt tgaattcctg attcatatag tcaaaaactt ctttagcaga
    12661 tacgtagcgg ttacgcttca ctaaataagt taacaatgct tctctttttt tagtatattt
    12721 aaaaccgtta tttttcaatg tttctaatga ttgttcgatc aatgtctgtt ttttcatgac
    12781 ttattcctcc aaatcaaaat gattacgaag tacgcattct agagtatggt ataataaaca
    12841 taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcgttacttt
    12901 ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa ctagcgcaag ctactatggc
    12961 tcagtatcct accgttgagt tcaacctatt ccgcagaacg ttcgtgacag atgaaaaaac
    13021 attaaggcag gcattgcaag atgcattaga agaaaaagca cttgtcctgc atacattaat
    13081 caatcaagaa ctgatcgaga taacacgttc tttttgtgaa gaacatcaat tatttagttt
    13141 tgatgtgatg acgccaccga tatcagaaat tgaacgttta actggtatca agccaattcg
    13201 gcaacctggt gccctccatt tattgaatga gaactatttc aaacggatca aagcaatgga
    13261 atttgctgta aaatacgatg atgggaaaga tcctcgtggt tttttagaag cagatgtcgt
    13321 tttgctaggc gtatcccgaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa
    13381 tttaaaagtt gccaatttac ctttgatccc acaagcacat attcccaaac agctttggga
    13441 gatcgatcct aaaaaaatcg tgggactaac gaataaccca gatattttaa ataatatccg
    13501 taaagaacgg atgcgctctt acggattaaa ccctgatact gcttattccg atatcgaaaa
    13561 aattagagct gaacttgatt ttgcgaatga tctttatgaa aaactaggat gtgtagtgat
    13621 caatgtggca tctttatcta ttgaagaaac ggcttcgctt attttgaatg ctttagacct
    13681 agaagatcat agttattatg gaactgaaac aacagaaaaa agcgattaat catttttgtc
    13741 ttttt
//
LOCUS       DAZUKA010000077        13536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000077 DAZUKA010000000
VERSION     DAZUKA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.077"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1447
                     /locus_tag="ACG8PN_001790"
     CDS             20..1447
                     /locus_tag="ACG8PN_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4861932.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1539..1904)
                     /locus_tag="ACG8PN_001791"
     CDS             complement(1539..1904)
                     /locus_tag="ACG8PN_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4861933.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1901..2266)
                     /locus_tag="ACG8PN_001792"
     CDS             complement(1901..2266)
                     /locus_tag="ACG8PN_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4861934.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2458..2706)
                     /locus_tag="ACG8PN_001793"
     CDS             complement(2458..2706)
                     /locus_tag="ACG8PN_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861935.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2705..2833
                     /locus_tag="ACG8PN_001794"
     CDS             2705..2833
                     /locus_tag="ACG8PN_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4861936.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2890..4584
                     /locus_tag="ACG8PN_001795"
     CDS             2890..4584
                     /locus_tag="ACG8PN_001795"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4861937.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4684..4857
                     /locus_tag="ACG8PN_001796"
     CDS             4684..4857
                     /locus_tag="ACG8PN_001796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861938.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5235..6368
                     /locus_tag="ACG8PN_001797"
     CDS             5235..6368
                     /locus_tag="ACG8PN_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4861939.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6399..6719
                     /locus_tag="ACG8PN_001798"
     CDS             6399..6719
                     /locus_tag="ACG8PN_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4861940.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6748..7212)
                     /locus_tag="ACG8PN_001799"
     CDS             complement(6748..7212)
                     /locus_tag="ACG8PN_001799"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4861941.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7344..8597)
                     /locus_tag="ACG8PN_001800"
     CDS             complement(7344..8597)
                     /locus_tag="ACG8PN_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4861942.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8626..8943)
                     /locus_tag="ACG8PN_001801"
     CDS             complement(8626..8943)
                     /locus_tag="ACG8PN_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4861943.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8969..9289)
                     /locus_tag="ACG8PN_001802"
     CDS             complement(8969..9289)
                     /locus_tag="ACG8PN_001802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4861944.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9753..11168)
                     /locus_tag="ACG8PN_001803"
     CDS             complement(9753..11168)
                     /locus_tag="ACG8PN_001803"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4861945.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11185..12501)
                     /locus_tag="ACG8PN_001804"
     CDS             complement(11185..12501)
                     /locus_tag="ACG8PN_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4861946.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12651..13349)
                     /locus_tag="ACG8PN_001805"
     CDS             complement(12651..13349)
                     /locus_tag="ACG8PN_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4861947.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4289 a         2753 c         2330 g         4164 t
ORIGIN      
        1 acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct
       61 gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct
      121 cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga
      181 agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca
      241 tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg
      301 tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat
      361 ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg
      421 gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct
      481 taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga
      541 acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga
      601 aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc
      661 tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt
      721 cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt
      781 tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga
      841 agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt
      901 tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc
      961 gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt
     1021 ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag
     1081 ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt
     1141 gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac
     1201 aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga
     1261 ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca
     1321 gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga
     1381 aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa
     1441 caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt
     1501 tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg
     1561 ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct
     1621 agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca
     1681 agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg
     1741 ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt
     1801 gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt
     1861 ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc
     1921 tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga
     1981 taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga
     2041 tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc agttttcttg
     2101 tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa
     2161 gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa
     2221 tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact
     2281 ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta
     2341 aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct
     2401 ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta
     2461 gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct
     2521 aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac
     2581 aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat
     2641 ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt
     2701 tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc
     2761 tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt
     2821 cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga agacattgga
     2881 ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca
     2941 gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca
     3001 ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg
     3061 aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt
     3121 aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag
     3181 ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac
     3241 aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat
     3301 aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa
     3361 ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac
     3421 ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg
     3481 cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc
     3541 aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa
     3601 gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca
     3661 ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa
     3721 gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca
     3781 gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca
     3841 gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa
     3901 ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat
     3961 ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca
     4021 tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctattgtc
     4081 gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat
     4141 ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga
     4201 aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat
     4261 attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg
     4321 ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct
     4381 aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc
     4441 tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt
     4501 cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt
     4561 gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta
     4621 ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg
     4681 cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaagctgc cctgcatcaa
     4741 gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt agacttatta
     4801 ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg cgtttagttt
     4861 cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa atttgactta
     4921 ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg
     4981 aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct aacggataca
     5041 actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc gaacacaaat
     5101 acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag
     5161 atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga
     5221 aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa
     5281 acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag
     5341 ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt
     5401 atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat
     5461 tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct
     5521 gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc
     5581 ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag
     5641 ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag
     5701 ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa
     5761 aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat
     5821 tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag
     5881 ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg
     5941 gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa
     6001 tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaagcggca
     6061 caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa
     6121 ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata
     6181 tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgcaatga
     6241 ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag
     6301 cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga
     6361 aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata
     6421 cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct
     6481 tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta accgggattt
     6541 tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt
     6601 tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca
     6661 tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag
     6721 aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc
     6781 ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa
     6841 cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct
     6901 gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag
     6961 ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa tgacgcccgc
     7021 tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt
     7081 attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga
     7141 tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt
     7201 atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt
     7261 ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca
     7321 cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc
     7381 gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct
     7441 agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc
     7501 catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca
     7561 ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata
     7621 ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat cgacaagttt
     7681 gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct
     7741 aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata
     7801 aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg
     7861 attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat
     7921 ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt
     7981 tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt
     8041 gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct
     8101 tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc atttccgctt
     8161 aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa caaaccgttt
     8221 gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg
     8281 gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa agcaatcaag
     8341 acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt ccatgattca
     8401 ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt attgatcatt
     8461 actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc ccgaatcgca
     8521 actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt ctccataaat
     8581 gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg cgttcaatcg
     8641 acgatacaga tcaatgatct cttttgctaa atctgtaaaa gctatagatg tcatcagatg
     8701 atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt
     8761 cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat ctttgatttt
     8821 ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca ttgcatcgct
     8881 ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt tttgatcttc
     8941 catttattgt atcctccgat aatttttatt atttgtttat tagtttttct gcttggtcga
     9001 gtactttttc accattcatc atgccatagt cagccatatt gattacatct aaaggaatgc
     9061 ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa
     9121 gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca gatacagcaa
     9181 agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg actaggagac
     9241 ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt ttaaacacgt
     9301 tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa aagcgcttta
     9361 aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact aaaaaagaaa
     9421 gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg tgcacaaaaa
     9481 aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca aaagcttgca
     9541 tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac tttgctattc
     9601 ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt tcaatcagct
     9661 gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca aataaccagt
     9721 aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc aggcaaatcc
     9781 aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa tgttctgctt
     9841 cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc attcattggt
     9901 gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc ttcttctgtt
     9961 gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc atcttgcacg
    10021 attcctttct catccatata ctgttcttca tattctcttc ccataccgtt ttctgttatc
    10081 agccaaggga tattatgata attttctttt agatacatgg ctatatcata catgattttc
    10141 ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc aaagacttca
    10201 taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg ggcgcgaacg
    10261 cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg aatcgtttct
    10321 aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc caggaactct
    10381 tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa caaatcatac
    10441 atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat ttcaggattt
    10501 aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac tgctttagcc
    10561 gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg ttcatgcggc
    10621 cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac
    10681 cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt agcataaaaa
    10741 tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc tgggacttca
    10801 taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc gatcatttca
    10861 cttatatgct gagcatattc ttcatctact atacagttct cataatcagt aaaaaaacga
    10921 gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag atccacatcc
    10981 tgctggtatt gttccataaa attagttgct acggctgggc catacccatt atgccaaaca
    11041 aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt ttttcctttc
    11101 caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt tggaatagta
    11161 attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat ttgctacttt
    11221 tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg taataactgc
    11281 accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg gccaagggac
    11341 cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa tcgttccagt
    11401 gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga tcggcatacc
    11461 aaaaataact ggttcattga tgttgaagat cccaggagca agggataact tggtgatttg
    11521 tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga tcgtgactcc
    11581 agacccgccc atactcatat acatatccca aaatggcata gtaataatgt tcggtacttc
    11641 ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca acggttcccg
    11701 tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt gcgcaataaa
    11761 catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc gttgtacgac
    11821 attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa atcccgcagc
    11881 cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag cagaaacatt
    11941 cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca accagcaata
    12001 aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg tatctgtata
    12061 ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta attcataaac
    12121 agttggatta acagataaat aagacatcac tgcgatgata ccagggaata caccatccac
    12181 tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg tcaagaaact
    12241 tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa aacgcaagaa
    12301 acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa
    12361 agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca ccatgatata
    12421 ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt tatccataaa
    12481 actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct ttttgtctat
    12541 tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt accaaatata
    12601 aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg tcaattagtt
    12661 tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg gacagtcatt
    12721 gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc tgtatgaata
    12781 tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa agcaattgat
    12841 tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc tgcaatcatc
    12901 gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca
    12961 atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata ttccgctgcc
    13021 aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg tatcatccga
    13081 tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg cttatcactg
    13141 acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag agcccgcaca
    13201 gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata ttcattcggc
    13261 agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt gtcagtagtt
    13321 acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc tgatgtattt
    13381 tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac aaaattgatt
    13441 aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac taggataaaa
    13501 ttcactccta gccttcgatc atttttattt tgaacg
//
LOCUS       DAZUKA010000078        13356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000078 DAZUKA010000000
VERSION     DAZUKA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.078"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..743
                     /locus_tag="ACG8PN_001806"
     CDS             45..743
                     /locus_tag="ACG8PN_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4861948.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(911..3550)
                     /locus_tag="ACG8PN_001807"
     CDS             complement(911..3550)
                     /locus_tag="ACG8PN_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4861949.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4169..5200)
                     /gene="queA"
                     /locus_tag="ACG8PN_001808"
     CDS             complement(4169..5200)
                     /gene="queA"
                     /locus_tag="ACG8PN_001808"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4861950.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5393..6568
                     /locus_tag="ACG8PN_001809"
     CDS             5393..6568
                     /locus_tag="ACG8PN_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4861951.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            6572..7213
                     /locus_tag="ACG8PN_001810"
     CDS             6572..7213
                     /locus_tag="ACG8PN_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861952.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            7210..8127
                     /locus_tag="ACG8PN_001811"
     CDS             7210..8127
                     /locus_tag="ACG8PN_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4861953.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            8124..8795
                     /locus_tag="ACG8PN_001812"
     CDS             8124..8795
                     /locus_tag="ACG8PN_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4861954.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(9336..9824)
                     /locus_tag="ACG8PN_001813"
     CDS             complement(9336..9824)
                     /locus_tag="ACG8PN_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4861955.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(10025..10291)
                     /locus_tag="ACG8PN_001814"
     CDS             complement(10025..10291)
                     /locus_tag="ACG8PN_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4861956.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(10291..10554)
                     /locus_tag="ACG8PN_001815"
     CDS             complement(10291..10554)
                     /locus_tag="ACG8PN_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4861957.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            11075..11215
                     /locus_tag="ACG8PN_001816"
                     /pseudo
     CDS             11075..11215
                     /locus_tag="ACG8PN_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(11357..13180)
                     /locus_tag="ACG8PN_001817"
     CDS             complement(11357..13180)
                     /locus_tag="ACG8PN_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4861958.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         3925 a         2851 c         2553 g         4027 t
ORIGIN      
        1 atacatggta tattggtgtt attaaagaaa aggagggaaa aatcatgcaa tcttctgcag
       61 aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg gggacaagct gttttactaa
      121 atctaatccc tacattgatt ggtattgcta ctattcttct tattatgatt cctattatct
      181 tgtttgccac caccatatat gatggatcag cgagcgtctc agaagcgaca acttcaggag
      241 gcggtagttc aggaggcggt ggcggaatta tttccagcat cattagcgct ttgttcatga
      301 gcggtatttc ttggacttat cttgatattt tacgtggaca aaaagatcag atcgaaccat
      361 tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg
      421 tgctgatctc gatctttaca acattatggg gtttgctatt agttattcct ggaatcatta
      481 aaagctattc ttattctcaa agctatttta tttactacga tatcgtccaa gaaacaggac
      541 aaacacctaa agtactggat acgatcactg caagtcgaaa attgatggat ggttataaag
      601 gacaactttt ctggttagat ctttcattta tcggctggca tatcttagca ttagcaactg
      661 ctggtattgg ttatctatgg ctcagcccat acatttctgc aacaaaagca gcattttatg
      721 aacaattgcc aaaagatatt taaaagagag tgtgacttgt gaccgagggg gtggcaggag
      781 aacaccgttt atcgagaggc agtaaaagaa acattcagcc cattagcaaa aaggaggtgt
      841 gacaacattc atgtcacacc tcctttttgt atagttccag taaaaagaaa atcaacttag
      901 aaacagttaa tcattcctct tcttctgctt ccggtaatcg atggaacttg atcagttcat
      961 tcaccgcgat tggtacgata gataaaatcc ctaccaagaa ccagtgattc aaactgatgt
     1021 gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta
     1081 atgccagtaa cgccatttct accaaagctt tattggtcgc taaattgact ttgaagatcg
     1141 attggctgct gcgtacattg aacacatgca gaatcgatga ataagctaag atcaagaatg
     1201 caacagtttg accgacatgc tgacttgcgt ctacccatgg gctgatagta tcgacataac
     1261 tacctaaata ataaccaaac aaggttacga tggtgaagac ccctgcattt atggcaattt
     1321 tttgcaataa acctcgagca aagatccctt catttttcgg aatgggtggc tgatccatga
     1381 tgccgtgttc cgctggctct cttcctaaag caaatcctgg aagtccatca gcaactacat
     1441 tgacaaacag tagttgcaca gctgtaaatg gtgctcccca tcctaacagc atcgcaatca
     1501 aaacaataaa gatctccgaa atattacagc ttagcaaaaa gttgatagct ttacgaatat
     1561 tctgataaac cgaacgtcct tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg
     1621 tcaaaatcat atctgcggca ctttgcgcca catctgttcc tgtgatcccc atggcacacc
     1681 caacgttact tgctttcaac gctggtgcat cattcacacc atccccagtc atcgctacga
     1741 ctgcacccgt acgttgccaa gatttgacga tccggatctt gtcttctggc gttacccgag
     1801 catagacaga gagagctttg acacgcgcat ctaattcttc gtctgataat tgatgcagtt
     1861 ctgatcctga aagtgcttct gacggatcat tcaagatacc taattctttc gctatggcac
     1921 tggcagtcac cacatgatct ccagtgatca tgactgtttt gatgccagcc tttttcgctc
     1981 gcttgattgc tcctttactt tctggacgtg gtggatcgat cattccgatc agaccaatca
     2041 aacgtaagtc tttttccagt gcttcagaag aaatatcttg cggttcttcg tcataagtag
     2101 cataaccaac agcaatcact ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg
     2161 cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac atcgaatgct cctttggtga
     2221 ttgagatgta tttttcgccc atttggtgaa ctgttgtcat catttttcgt tcagaatcaa
     2281 aaggaagctc gtttactcgt ggatattttt cttctaattc tgcctttgta tgataattct
     2341 cttcaacagc ccttacgatg gctacttctg ttggattacc tttgataaca aggtcgtcat
     2401 cttcttgctc gaccgtcaca tctgtacata gtgcagccat tttcaatatt tccatcgcgc
     2461 tgttcgtcat tgaatcttcg atattcgtta cttcatcttc tttgctccat acccggcgga
     2521 cgcgcatttc attttgtgtc agcgttcctg ttttgtctga gcagataaca ttagcggttc
     2581 ctagcgtttc aactgctggc aactgtcgga tgatcgcatg tttcctcgcc atcttctgca
     2641 caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt ttccggtaca gctgcaacag
     2701 ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc tccttgcagt tcaccaatca
     2761 caaatactaa agccgcagcc gctaaagcga tcatactgat tcgtttgcct agctgattca
     2821 atcggacctg taaaggtgtt ttttgcatag tattttcgtt caaaagatcc gcgatcttac
     2881 ccatttctgt agccatgcca atggcagtga cgatcgcttt cccacgacct gcagcaaccg
     2941 tacatccttt aaataccata ttaagttggt cacctaatga ctcatcttct tcagcaataa
     3001 atgctgcatc tttttcaact gcttcacttt ctccagtcaa cgccgattct tctacacgta
     3061 attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc atcaccggac tctaaaacaa
     3121 gaatatcacc cactaccagt tgctctgact caacagtggt cactttgccg tctcgtatga
     3181 cagttgccgt ttgtttattc atatcttgca aggattccag tgctttttct gcgtttcctt
     3241 cttggacaat cgccaatacc gagttgatca caacgatagc gatgatcagc agtccttcaa
     3301 ataaatcccc atgttctgta gcaaatgcgg tataaaagga aatcaccgct gctacaagaa
     3361 ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt tttgatcatc gattctttcg
     3421 gcgcctcatc gaatttattc aatccctgct tttttcgccg ttcatccact tcttgtgaag
     3481 acaacccagt atctggatct gcttctgtta ccttctttac ttcttctatt gtctgcttgt
     3541 attcttccat cctattcctc ctttaaaatc ttcgtacaaa aaaaggtact tattttacta
     3601 taacatcctt tttttattta cttcaaataa agggcttatc gattatttgt taagaaagcg
     3661 taaaatttaa agtgcttata tttgcaaacg ctaccaaagg atgctattat atagatgtaa
     3721 ggaaggagga taccatcaaa tagacagaag aatttctcga tagaacagtg taagcaaaca
     3781 aagttgttag aagtacaggt ttcacccaga catgcgtgta gagtacaaac ctataaatag
     3841 tgaatgcgta tggatgtttg gatcacgaag tatccgaaac aagaagtaat gcgatacgta
     3901 acaattgaat aacgtaaacg tctgaaaatt gaataggaac aagagatgtt tcaaatgttg
     3961 aagatgtaac gaaaacaaaa tttttgttta acatgaagaa attcaacacc gtgctttgca
     4021 tgtatgatgg aaagttacaa tcatttctgt ttatttgaat ccttccgagg tacaagaaag
     4081 ccgagcttat cgagagctcg gctttttctt tgtcataaaa tctttgtaaa gcttttttgt
     4141 tattcaaaac tagcggcttt tcataacctt atttcacgaa catcgcatct ccaaaactaa
     4201 agaaacgata gcgctcttca atcgcatgct ggtaagcaga taaagtcaat tctcttcctg
     4261 caaaggcact aactaacatg actaaagtcg attttggcaa atggaaatta gttgagaaag
     4321 cttctaccac tttgaattca tagccaggcg taatgaaaat atcagtccat ccgctgtctg
     4381 ctttgatttc tccattaaat ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc
     4441 cgacagccac gattttcccg ccatttttac gtacttggtt caatcgctca gccgcttctt
     4501 ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc aattttatct acgctgacag
     4561 gtcggaatgt acctaaccct acatgtagtg tcagatagac taattcaaca ccctttgctt
     4621 cgatctcatc aagtaattct ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac
     4681 cattttcttt cgcatagaca gtttgatagc gatctgggtc ttccagacgt tctttgatat
     4741 atggaggcaa tggcatttct cccaatgatt caagaatctc tagaaaaatt ccttcatact
     4801 caaagtcaat gatccgtccg ccgtgttcca attcttcttt cactactgct tgcaatctgc
     4861 catcaccaaa agagattctt gtcccaactt tggcacgttt cgcaggtttg atcaatgtct
     4921 cccacgtatc tccttgtgta ttcgttagaa gaaggacttc taaatgcgca ccagtatctg
     4981 gtttttctcc atataaacga gcaggcagaa ctcgtgtatt attcataact agcgcatcac
     5041 caggctgtaa ctcttcaatg atttcatgga aatgtttatc ttccatctct ccagttttcc
     5101 gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt
     5161 cttccggcaa gtcaaaatca aaatcttctg ttgttaacat atcgtcactc actttctatc
     5221 atgtgcttca tcattctatc atgtttttcg ttctgttggc aaaatcagca ctttttcact
     5281 tctatttttt gttttcctaa ataaaaaaac taccctattt gtattttttc attgttttag
     5341 gcatactagt aatagatagt tcttcattga aaatagaaaa agaggtggca ccatgattga
     5401 atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc gctgtagagg acgtcaatct
     5461 ttcatttgaa aagggtgagt tcatttgttt tatcggtacg agcggaagcg gaaaaacgac
     5521 gacgatgcgg atgatcaacc gaatgactga tccaacacaa gggaaaattt tgattgatgg
     5581 aaaatccatc caagaaattg atccagtgga attgcgacgt cagatcggct atgttatcca
     5641 aagtattgga ttgatgcctc acatgacgat ccgtgaaaat atcactcttg tccaaaaatt
     5701 gttgaaagtc agtcaagaag aacggaataa aacagctgaa aaaatgattg atcttgtgga
     5761 attgccccgt gagatgttag atcgctaccc acatgaatta tctggtggtc agcagcagcg
     5821 tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc atcttgatgg acgagccatt
     5881 cggtgcgctt gatccaatca ctcgtgattc cttgcaagac cttgtcaaag atttacaaga
     5941 gcgtttggga aaaacgatcg tatttgtaac ccatgacatg gatgaagcga ttaaactagc
     6001 aagccgtatc gccatcatga gtgaaggccg tgtgatccaa tttgatacac cagaaaatat
     6061 tttacgtcat cctgcaaacg atttcgtaga agagttgatt ggagaagatc gcctactaca
     6121 agccaagcca aatgcgacaa gagtcggcga agttatgcta aatacagcaa tcacgattac
     6181 accggaaaaa tcattacaag aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt
     6241 gttagttacc gataattcga atgtattaaa aggatttatc gatgtagaaa cgttgaataa
     6301 aaaacgagga aaagtatcca gtgtcggcga cattttgaat aaacatgttt tttacgtaaa
     6361 agaatccgcc tatcttcgtg ataccttgca acgcatttta aaacgcgggc tgaaatacgt
     6421 gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg actcgtgcat cattagtgga
     6481 tatcgtctac gatgtgattt ggggagaaga tcccgcaact actgatgttg cagagtcagc
     6541 tactcctgaa gaatctaagg aggtctgacc tatgcagcag ttttttcatc agcacggttc
     6601 agaaattctc tcaaaaggtt ttgaacatct ttatatttca ggacttgctt tgatcattgg
     6661 tatcatcatt gctgtaccgc ttggtgtctt actgacacgt tttccaaaag ctgccacgat
     6721 tgtgattggc ttaaccagtg ccttgcagac aatcccttct cttgctttat tggcattgat
     6781 gatcccgcta tttggcgtag gtaaattacc tgcgattatc gcactcttta tttattcatt
     6841 attgccgatt ttacgtaata cgtacattgg gatgaaaaat gtcgattgga attatcggga
     6901 tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt ttttccgttg aattgcccat
     6961 tgctatgcca acgatcatgg ctggtattcg tttagctgcc gtttacgtga ttgcatgggc
     7021 tacacttgcc tcttatatcg gcgctggtgg tttaggcgat ttgattttta gtggactgaa
     7081 taattaccaa ccagatctca ttttggcagg tacgatccct gtaacaattt tagcactatt
     7141 ggctgattgg cttctaggtc tcttggaaca ccggctgaca ccgatcgcac ttagagagga
     7201 gaatgaagaa tgaaacgtat actaaaaatg accatggttc ttttctctct tcttatactt
     7261 gcgagttgtt ccttccctgg tcttgccagc aatacagatg aagacaccat ttcgatcaca
     7321 ggtggaatca cttcagaagc acagatctta gcaagtctag ttgccggaat ggttgaacat
     7381 tatacagata aaaataccgc aatcatcaac aatctggcta ctacaactat caaccatcaa
     7441 gcgatgatga acggagatgc ttcaatttca gcggctcgct acacaggaac agatttaacg
     7501 actacattga accttcctcc tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat
     7561 gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct atagttttga aaatacgtat
     7621 gtctttcttg ttcgaaaaga caccgcacaa aaataccatc tatctaaagt cagcgatctg
     7681 aaaaatgtag cggatgaatt agtagctggt gtagatactt cttggatcaa tcgtaaagga
     7741 gacggttatg acggcttcca agaaacttat ggcttctcct tcaattcgat attacctatg
     7801 cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac
     7861 tctacggatg gacggatcgc cagttatgat ctagtcatgt tggaagatga tcgtcaattc
     7921 ttcccaccat atgatgctgc tccagttgta gacaacaaga tattagctga tacgcctcat
     7981 ttaaaagaag ccttaaataa gttaggtcat accatcagta cggaaaaaat gcaggaattg
     8041 aactacgaag cagataataa tctagtggaa ccatcagtag tagcacaacg tttcttacaa
     8101 gaaaaccact attttgaagg gaagtgaagc aattggataa aatgaatgtc tttcaacaat
     8161 ttttctatta tttccaggaa aatggctcct atatctttgc acaatttatc cgtcacttcc
     8221 tgatttctat ctatggtgtc ctctttgcag cagttgtcgg gattccggtt gggatcatga
     8281 tttctaggag acgtaaatta gcaaactggg tgattcgttt ggcgaatatc atccagacga
     8341 tcccttctct agccatgatc tccattttaa tcattggatt aggcctggga gtcaatgtcg
     8401 tgattgttac tgtatttttg tattccctct taccgatcat caagaatacg tataccggga
     8461 tgatccaagt agacaaaaac atccttgatg tcggcaaagg catggggatg actgctagac
     8521 aacgactatt catggtagag ttaccactct ctgtttctgt tatcatggca ggcataagaa
     8581 acgcgttagt tgttgccatc gggataaccg cgatcggtgc attcgtcggt gctggtggtc
     8641 ttggagatat cattatccga ggaaccaatg caacagaggg cacatcgatt attttagcag
     8701 gtgcattgcc aactgcatta atggcgatca tcacggactg gcttttaggt attttggaaa
     8761 gacggctaga tccagccagc cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc
     8821 tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt catttttaag gcaataatcc
     8881 actctgattc ttccctcctt atgggcaaag agttgctttt cttagctatt tatccattcc
     8941 gtatactcca acttcatact gccaacgggt tcatatctgc atccaccgac taagtttttt
     9001 tcttatccgc taaggcattt tgccaatcca ttttcccatc actcgtcatg gaattcatac
     9061 cacatgatct ctaaatactc gtctgcttgt tctttgaatt gggcatcaat tcttacatcc
     9121 tttggcgtgg ctttcattta agccttttcc atcagtcctc gattgttcga tgccttcagc
     9181 tgcatcatca aaactggtga ctgccgtatc ggtaacttcg atcatctttt atacaatcgg
     9241 gaacttgcca tcctcatcct tggctaaaaa catatctagc cattccagct tctcgccatg
     9301 ggaggtttgc ggttcctaaa accggtacac gattcttatg gactgcatcc gtcgcaacta
     9361 gacttggcga tacgatgatc cttcaccaga agagcacccc cgtaaaccaa ttgatcgata
     9421 tattgccaat aagaaaagac attggcgtaa aagatatcga tccctcgtga tgcattgccg
     9481 ttgatactgc tattcatggc cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca
     9541 ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta aaggaacgac actggtattg
     9601 tatttcgtat ctgtatcttt tccaaaagac catgccaacg aatcctctgg gaaccaataa
     9661 gacgactccg gttgattgtc cattgctttt ttcaacatac ttttggctgt ttctcgataa
     9721 acgacatttc caattgtctt cgcctcactt tggcgataat cgatggtcgt tgccactgcc
     9781 aacactaaca acgatgctcc aattttactg attttccttg tcacacttct tcctccttgt
     9841 tttacataaa cccgtcaaca ttccattcct aaactcaaac aatgaccctt caaaggtgga
     9901 gatataggaa caaattaaag aaatcatgca ataaatccaa gaaggttggt tttagaacaa
     9961 aaaaagagtc tcctttttgg ggagactctt tgttaactat ctaaaaaggt tggctcttct
    10021 cacttcactc ataatgtgtc caggcagaat aaatttctac aactttattt tcctcatcaa
    10081 ctttgtaaac cactcgatgc tgtctattca gcctgcgtga gtatctgcct tcatgtgtag
    10141 gtctcagctt ttcaaaagac tgcgtgggca tatatggatc ttccttcaat gtctgaatca
    10201 cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt tcttaaatcc actttcgcag
    10261 agtttttgat tgcgactgta tagttactca tcaaagagct tcccaatcaa tatcatcgat
    10321 attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact ttatctaaag tacctgtttg
    10381 ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc agcccaatca atacagcatt
    10441 gtttccacta cgatcactaa taatctcgat ttcagtatga ttttcattta cctctttcaa
    10501 taactgataa aaatttttac gagcagtgct aggattcatc gttgtattag ccattgtctc
    10561 tcctcctttt tctgatataa ttatagcgta cttgattaag tacgtcaaaa aaatacaata
    10621 aagcacttaa attcagcaaa cttttcctct tttttacata gagaaaaaga ccccttttta
    10681 gggaggatct ttgtccacgc tcacttctta ctcctttacg actagcggta gccaaacgta
    10741 aagtaattgg taacaacgta tcatctttag caaaggtcga atccaaagcc tgtgagtcta
    10801 tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt gacttgattg atatcttgaa
    10861 taacctgagg atacgcacga aagatttgta acggttattc aaaactgatt ttggccgtca
    10921 agtcttctac tgcctcaaaa atgattgcct ctttcgtctc ctgtcattca tgttcactat
    10981 cgatttgata cttgcttaga ccgttgggtt tgtgaatcac caattaaaaa tggcactgag
    11041 ttatggtaac aaaaaatggt cacttgacga ggctgagatt cctacttatg aagacacatg
    11101 gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata ggaggctgta cattatttgg
    11161 caaaggattc ggaaattatg atcaagacat caaaatcaaa atgttttaca gctaaatagg
    11221 aagaataata acatttcata cgctgttccc atctttaatg gcttatagag ggcaccttta
    11281 gctgagaata agacaaaaca gaccctctag cttcaattaa gagctagagg gtctgttttt
    11341 gattttaaaa cttggtttat ttcttcaaat ggtagctata ggtgctgaca acgatatttt
    11401 cacgccaaga caatcgcaat cttaaccgca aacttgtttg gttatggaga atattctgcc
    11461 agcgtctgtt tggtacaaat tgaggaatca acacagtagt tgtatagttt tgtttcgctg
    11521 catttttact tacgatatct acataacgga taattggatt ttcaatcgag cgataagaag
    11581 agtggacgat tgaaaaacgg acatctggga aatgcttttt aaattctgct tcgatttctt
    11641 tttctttttc gacatcttcg tctagcgaaa catgcatcgc aacgacataa tcaccgatcg
    11701 aacgtgcgta attcaacgca ccgatattta cccgtgtcac attcccgact aatacaagaa
    11761 cggtattccc gtcataatca tgtagttttg catcattttc taaacgaagc tgttctgcta
    11821 cttttttata atgatcatgg atcttataaa aaatgaacat aacgattggc ataatgatga
    11881 agaaaggcca aatgtcccct aaccgataga caaacaaaat cgcaatgatt gcatatgaga
    11941 tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca tctttttcct tcttttttcc
    12001 atttgacgac catccctgtt tgagataaag caaatggaat gaacacgccg atagagtata
    12061 acggaatcaa gcgttctgtt gagccatgga aaataaacag caatacgatc gaacctagcg
    12121 ctagtgtgat aatcccattg gaatagccta gtcgatcccc acgatcttga tacatgtgag
    12181 gcatgaattt atcttttgcc agattgtatg caagaactgg aaaagcagaa aaaccggtgt
    12241 tagcagcaac tgctaaaatc agcgctgtag caaattgaag gacgtaatac aaaataccat
    12301 gtccaaagac agctttacca atttgagaca aaacagtgac ttctttttca ggaacgatcc
    12361 cataccaata gttgataaat gtgatcccca cgaagaagaa acctaagatc aatgccatca
    12421 tcgtcaatgt agcagcggca ttttttgctc ttggcttctt aaagaatggt acagcattac
    12481 taatcgcctc caccccagtt aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag
    12541 cgatagaaac acccggcaca gccgctcctg gaagtgcagt agcttgcaat ggcacagcac
    12601 ctgtcacgat attatataaa ccaacaacga tcagtaacga gatcacaagg ataaatgtgt
    12661 acaccggaaa taataggaac gatgcagatt ccctcagccc acgaagattc agcatcatca
    12721 gtagcaaaac gattgtcaca gaaatagcga cttgatgacc gtagagtgct ggcaccgctg
    12781 atgtgatcgc ttcagcacct gctgagacag atactgctac tgtcaacatg tagtcgatca
    12841 ataaagagcc accggaaatc aagccagcat tctttccaag attctcactg ctgacgacat
    12901 atgctccccc gccatgagga taagcatgga tgatttgacg atatgatagc gttaatgaaa
    12961 tcaataaaat aataacaaaa gcggcaatgg gtagcgaata ccagatggct gcagcagaca
    13021 aagcgactaa aacgacaacg atttgttccg taccatatgc aatagatgat aacgcatcag
    13081 acgacaataa tgccaaagca gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt
    13141 ttaaaggctt acctactaac aaccttttta agtaatccaa tttctaaact cctttacaca
    13201 caattttact tcaagtttta tagtcgaaaa tttatagtaa acaagatgtt atagtacgct
    13261 cactgacctt aaaagtcaat aaactttaag aaaataattg gaaaaaaaca gaaaaatatc
    13321 aagcgctttt tcaaacgctt taatacagca aaaatc
//
LOCUS       DAZUKA010000079        13254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000079 DAZUKA010000000
VERSION     DAZUKA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.079"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(184..256)
                     /locus_tag="ACG8PN_001818"
     tRNA            complement(184..256)
                     /locus_tag="ACG8PN_001818"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Lys,seq:ctt)
     gene            637..1206
                     /locus_tag="ACG8PN_001819"
     CDS             637..1206
                     /locus_tag="ACG8PN_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4861959.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1193..2053
                     /locus_tag="ACG8PN_001820"
     CDS             1193..2053
                     /locus_tag="ACG8PN_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4861960.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2243..2947
                     /gene="yycF"
                     /locus_tag="ACG8PN_001821"
     CDS             2243..2947
                     /gene="yycF"
                     /locus_tag="ACG8PN_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4861961.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2952..4787
                     /gene="walK"
                     /locus_tag="ACG8PN_001822"
     CDS             2952..4787
                     /gene="walK"
                     /locus_tag="ACG8PN_001822"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4861962.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4784..6100
                     /gene="yycH"
                     /locus_tag="ACG8PN_001823"
     CDS             4784..6100
                     /gene="yycH"
                     /locus_tag="ACG8PN_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4861963.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6101..6970
                     /locus_tag="ACG8PN_001824"
     CDS             6101..6970
                     /locus_tag="ACG8PN_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4861964.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7025..7834
                     /locus_tag="ACG8PN_001825"
     CDS             7025..7834
                     /locus_tag="ACG8PN_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4861965.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7937..8566)
                     /locus_tag="ACG8PN_001826"
     CDS             complement(7937..8566)
                     /locus_tag="ACG8PN_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4861966.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8736..10028
                     /locus_tag="ACG8PN_001827"
     CDS             8736..10028
                     /locus_tag="ACG8PN_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4861967.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10104..10766)
                     /locus_tag="ACG8PN_001828"
     CDS             complement(10104..10766)
                     /locus_tag="ACG8PN_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4861968.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10991..11275
                     /gene="groES"
                     /locus_tag="ACG8PN_001829"
     CDS             10991..11275
                     /gene="groES"
                     /locus_tag="ACG8PN_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4861969.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11326..12951
                     /gene="groL"
                     /locus_tag="ACG8PN_001830"
     CDS             11326..12951
                     /gene="groL"
                     /locus_tag="ACG8PN_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4861970.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4414 a         2282 c         2852 g         3706 t
ORIGIN      
        1 ggggaagtac atactgtgat aatctacagg tttattgact gattaggatg ttattaaaaa
       61 ataaaatact aaaatgcata cccgaatttc atacccgaaa aataaaaaat gccaccacgt
      121 acactctttt cgaaactacc taagtagcca agaatgtact ggtgacgtgg gtttggcacc
      181 caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca
      241 actgagctaa cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat
      301 gcaagactac atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat
      361 agattattca catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga
      421 gaacaaaccc taacactttt ttcatttttt actcctttcc cgccgtctcc ccggcgggtc
      481 tcctgttctt aacgataaaa gacaagaagg cttagccttc ttgttttttg ttttcatgga
      541 atcccgctaa tcaaatcacc tagttaaagc aactagattg aatgtttatc ataagtctga
      601 tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta
      661 atggagtggg ggaaggaact agaaacattc cgtctgcctc actgggaaga actccctgac
      721 ttagaacttt atatggacca agtcagaacg ttagttgatc gttatctttc acctgtgata
      781 caaggagaaa agcatccatt gcttacctct tctatggtaa acaattacgt taaactaggt
      841 ttgataccag caccagttaa aaaaagatat aacaaagaac acgtagcatt tttacttgca
      901 atcactacgt tgaaacaagt attgactatt cctgaaatca aagaagggat attgttccaa
      961 ggaaaaacag taggaattcg tgaagcctat aatctatttt gcgatgaaca agaagcagct
     1021 gtgtggatgg tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca
     1081 acccctgtag aatatattgc tgtaagagcg gcaactttat cgtttgcaat gaaacttctt
     1141 gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa
     1201 caataaaatc gcaattttag tagattcagg tacggatgtg ccagaagaat tgataaaaaa
     1261 atacgagatg tatatgatcc cattgaaaat tatttataaa gatcaggtat atacagataa
     1321 aattgacatt acctctgaag agatctatca acgtttgcca gaggaaatcc ctggtacatc
     1381 tttgccggat ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag acggttatga
     1441 aaaggtactt gcggtaacaa tttccagtgg attgagcggg acatataatg tggttcgttt
     1501 aattgcacaa cagcaagaag aattggaagc atatgtacta gatacgaaaa atattggtat
     1561 tggtgcaggt ttttctgcga tccaagcagc aaaatggctg gaagaaggag tagaatggga
     1621 tcaactgatc agtaatctga atgagttagt cgaaagaaca aaagttttct ttaatgttgc
     1681 gacattggaa tatcttcaaa aaggtggacg gatcggttta gttgcttcta ttgtaggaac
     1741 cgctttaaag cttaacccaa tcatctcatg taatgaagaa gggatttatt acacagtagg
     1801 aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca aagaaaaaat
     1861 cggtaatgca aaggtcttca acttagctgt tgcccatggg gatgcgaaag aagaagcaat
     1921 tgaaatggaa gcgagactta aaagagagtt cccacaagca aaccaaatct actttggcca
     1981 gatttctccg gcattagtag tccacactgg tccgggatta ttgggtgtcg gtgtacaagt
     2041 tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt
     2101 attgaagtta gaacaaaaaa taataaagta ttttgcactt tcattttaat tttctctttt
     2161 gttttatagg caaagatgaa acaaaagcag aaatacggta taatgagaag gtatgacaat
     2221 aatatataag aggtggctct ttatgaaaaa agttttagtt gtcgatgatg agaagccgat
     2281 ttcggatatc gtcaaattca atttagccaa agaaggctat gatgtttata ctgcatatga
     2341 tggggaagaa gccttggaaa aagttgctga ggtagaacct gatttgattt tacttgattt
     2401 gatgctacct aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat
     2461 gccgatcatt atggtaacag ccaaagattc tgaaattgat aaagtcctag gcttagagtt
     2521 aggcgccgat gattacgtga ccaagccatt ttccaaccga gaattagttg cacgtgtcaa
     2581 agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag cacctgcgga
     2641 attgacgatt ggtgatttga cgatccatcc agaagcttac atggtgacga aacgaggaga
     2701 aacaatcgag ttaacacatc gtgaattcga actactcttt tacttggcga agcaccttgg
     2761 acaagtgatg acaagagaac atttattaca aacggtttgg ggttatgatt attttggaga
     2821 tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag
     2881 ccatccaaat tatttagtga cacgtcgtgg tgtcggttac tatttgcgta acccagaaca
     2941 ggagtaattg gatgaaaaga aaagtccgct tttttcgatc agtcaatttt aagatcgcta
     3001 ttacctttat tctgatttta ttgatctcga tcgaaattat tggtgcctac tttatacgtg
     3061 gtttagaacg ttccacgatc aatacattta ttaaagatat gaatcaaacg gtggaatcat
     3121 tagcaacaac aatcagcccg gaattgaatc gaaaagataa tgcagatgat gaagaggtaa
     3181 atgcgaatat caaaagattt attgaaaaca gtgcgactag cgatatcatc gaaatacggg
     3241 tcgttgacga aaaagggatc atccgaggaa caaccgatgt caatgaacaa agcgctgtgg
     3301 gaaagaaaaa tgattatgtt gatatcaatg atttcacgac taaacgttat gttgctttag
     3361 ataacgacga taaacgggtg aatatcaatg ttcaaccgat cctgtccccg accggcgatg
     3421 ccgttatcgg ggctctttac gtcaaaagta atatcgaaca aaaatattcg gaaatcaata
     3481 acacagcagt cattttcttt actgcctcac taattgcggc atttatttca atgatcgtat
     3541 ctgttttagt tgctcgttcg attacacagc cgatcggtga aatgagagaa caagccattc
     3601 gaatcgctag aggagactac agtcggaaag tcaaagtgca tggacaagat gaacttggac
     3661 agctggctga taccttcaat cagttagctg aacgtattga agaagctcaa gatacaatgg
     3721 aagctgaacg caatcgtttg gatagcgtgc tatcccatat gacagacggc gtcattgcga
     3781 cagatcgcag gggtaaagtc attacgatca atgatatggc tgtttccttg ctagatatca
     3841 aaaacgagga agcaatcgga caatcgattt tgacactttt agatatcgaa gaagaatata
     3901 cgttgcggaa attattagaa gatccaaacg aaatggtact tgaccgttca accagtacat
     3961 tggaaagtga tcagatgatc ttgcgagtag acttttctat gatccgtcgc gaatcaggtt
     4021 ttatcagcgg attagtagct gtcttgcatg atgtgacaga acaagaaaaa acagaacggg
     4081 aacgtcgtga attcgtctct aatgtttccc atgaacttcg tacaccgttg acaagtatgc
     4141 gtagttatat cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt
     4201 ttttgaaggt aacgctagat gaaacagacc gaatgattcg aatgatcaat gacctcttaa
     4261 atctatctcg catggataca ggtaataccc agcttcaatt agaatatgtc aatttcaacg
     4321 agttagtgaa ttttgttctt gaccggtttg atatgatggt gggaaatcag gaaaaaaatt
     4381 ataagatccg tcgtgaattt acgcaaagag atctttgggt cgaagtagat acagataaaa
     4441 tcatccaagt agtcgataat atcatgaata acgcgatcaa gtattcacca gatgggggaa
     4501 cgattacttg tcgcttatta gaaacgcata ataacgtggt tttaagtatc accgatcaag
     4561 gattggggat tcctaaaaaa gatctgaacc gagtgttcga gcggttctat cgagtagata
     4621 aagcacgtgc aagagcacaa ggtgggacag gacttggatt agctatttct agagaagtga
     4681 ttaaggcgca tcgaggcgct atttgggcag agagcaagga aggaaaaggg tcgacttttt
     4741 atatctcgct gccatatgaa ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa
     4801 agtcgttcgt ataggactca ttttgatggt tgcgttaagc atatacttct cttatgcaat
     4861 ctggctgagc ccagcaggaa aaagttcaat caacgtagat gagacaaaca ctcaaatgat
     4921 cgagtctcaa agcaatcgaa aaacttccga agtctttctt ccgcttcatg ttacatggca
     4981 ccattcggga atgatccaag aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat
     5041 catcgataaa gcaagatttg gaaagctttc tgaagtagtt tcaggagaca aatcacagtt
     5101 tgaacaggtg aaaaatctga actctggttt tgagttggcc tataatgcgc cttttttgct
     5161 aagagaatac aaggaagcat ttgatttaga tttagatgtg tcgaacttgg caaaagaaag
     5221 cgaaaatatg tattttacta gaatacaatt cgatcaagga aataaaaaaa tccgattttt
     5281 gaattattcg aaccatctcg tatatgaagc aaatatttcg ttgaattgga cagatatcaa
     5341 taaagaatta acagcaaaag acacaaaatg gacagaaatg acaacagaac catcgacgat
     5401 cgacacgcaa tatgctacga aacattcgat caaactgaaa aagtatagtt atatcctgtc
     5461 acagcaaccc tatacacttt ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa
     5521 taatgatgaa agcaatgaat tgatttttta cggtgggaat gaaacaatgt ctatccattc
     5581 ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt
     5641 attttctgaa agtttcccct atattagcaa attaggcagt tctttaggga acctcagata
     5701 ttttgaccgc agtgacaata cgatcgatta tcggatattc gtggaaggct tcccggtttt
     5761 tgggacagac agcagaggac agattggtgt ggaagttggc ggtgaaacga atggttctgt
     5821 gcaagtgaat atccaaacca gtttgaatac gattcaagtg ccgatacctt cagacgaaga
     5881 agtagaactt gcaagtacga atgaaatcat ccagcagtta aaagctaagg gagcagatag
     5941 caacaagatt ggttcagtga tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg
     6001 tgtcgttgat ctcttacctg aatggtatgt gaaatataaa gataactggt attcagctaa
     6061 cgaccttctg gaaaaattac ctggattgga ggcgaattaa ttggatttca aaaaaatcga
     6121 atggatattt ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca gttatcatga
     6181 cgcggtatac caagaaaaca atgtcattcg ctctaatcag actgaatcaa tccaacaacg
     6241 tctggctagc gacgatatca cttacacggg gaagatttcg tctgagaaca agcaaggcta
     6301 ttatcttagc gcggagcaga ccaatcttag cacttcatta gcgaattatc gcaaggctgg
     6361 taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt gatgttttga cgaaatcttt
     6421 atccagttca gatgctgaaa agaacgtgat cgatccagat aaaattagta gttccttgga
     6481 agatttttta tctaacaaag acaatatttt attcggtaat gattatgtgt acacgaaaaa
     6541 cttttctcat ttagaagaag aaccactaga aatccaagca agccaagagt atgaaggcat
     6601 cccattcaat gatgatacag caaaaatcgt tctttcttta gaaaaaaacg ataatgatta
     6661 tagcatcacg aaatacatgc agacgcattt atctgatatc gagcagctgc gcgaaaagac
     6721 ggaactgtat acggaagagg aagcaatcaa tacattgtac atcaataaca aaataccaag
     6781 aggatcaaaa attttatggc gtcagctagc ttattcctgt attctgaaag tccgggaaaa
     6841 aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca agcgataaag tggtccaaat
     6901 tgagagtgtc aacgctttca gcaatacgat cgtgacaaac aatactgtac caaaagtgga
     6961 agatcaataa aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg gaagtaggag
     7021 tcaaatggct gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg gaagttcggg
     7081 taattcactt tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg
     7141 taaaaaaatc acttccttga tggctcaaat tgatcggaca ccggaccaat tagatggaat
     7201 acttgtgacg catgaacatc gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta
     7261 tcatttggat gtttttgcca atcagcagac ttgggaagcg atggaaccac tgattggaaa
     7321 tgtaccagtg gaacaaaaac atctatttga gatggggaaa gtcagaacat tcggcgatat
     7381 cgatattgag agtttcggcg tctcccatga cgcagcggct ccgcaatttt atcgtttcca
     7441 taaagaaaat cggtcattcg tcatgttgac ggacaccgga tattgcagtg attatatgcg
     7501 aggtgtcatt gaaaatgccg atggttattt gatggaaagt aatcatgatc ttgagatgtt
     7561 acgcatggga ccttatccgt ggaatttaaa acagcgtatt ttaggagata gaggacatct
     7621 ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat
     7681 ctatcttggc catcttagca aggaaaacaa tatgaaagaa cttgctcatt taacaatgga
     7741 aaatgttttg aaagaaaaag attttggcgt tggacatgat tttgaaatct atgatacaga
     7801 tccagatgaa gcaactgagc tattttcgat ttaaacagtt caatcaataa aaatataaaa
     7861 agcagaagat aaaatcaatc agggttggtt ttatcttcta tttttatttt tttagcatat
     7921 taaaaaaata accgtattat cgactgacta cactgatgtg ctgcttttca tgtttctcat
     7981 ccagtatctc atctactaat gcaatcgcgt aatctgccat gctgatcgta ctgtcgccag
     8041 cagcgtttct ttgtaaataa tcatcactta acaaataagt acctgtacgt ttgccatgtg
     8101 gatcaaataa agaagctgga ctgatataag tccaatgtag atttttagat tgtttcaatt
     8161 ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa gggtgcattt ttaggtactt
     8221 ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa cgaagaggca cccccaacga
     8281 caattaagcg tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa gaagtttgat
     8341 gcatttcttc ttttcctttg ggagcgttga aagcatcaac gacaacatca aaagatgcaa
     8401 gatcagtagt tgtcaactca aataaatcct tttgtataaa cggaacatct gatggcaatt
     8461 tttctggatg acgcacgatt gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa
     8521 taaatgaacc tgcatggcca gtagctccaa tgagtgcaat tttcataaaa aacctccttt
     8581 tttattcctg tccattgtaa gcagttgctg aatattcgtc aaagaatccg atttttgaaa
     8641 agctcagtgc gcattccttt catggttcgt gatcattggg tatagttaaa ttaagaaaac
     8701 tgattgataa aaatagaagt tgaaggagat aagaaatgaa agagtggtta gatcgtgcct
     8761 ttttaccaat gataacatct gttgtgaatc ttagaccctt aactgctttg cgtaatggaa
     8821 tgacttacac gatcccaata attattgttg gctcgctgtt tttcttattt gctcattttc
     8881 ctgtggatta catcgctcag tggatagaca aaacaggatt ttctatgtat tttacacaag
     8941 tataccaggc tacatttgat gtgatagcct tttggatcgt ttttgccatt gcatacgctt
     9001 atgcaaaaga agaagacatc gatcgcttac cagtcggcat aacagccttg tctgcttatc
     9061 tactgcttat tcagccacta aatactcaga tagatactgt ctggacaggt gctgaaggga
     9121 tactaggagc agttacaact gggctcatca tcggatgggg ctatgcacgt ttagtaagac
     9181 ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa tgtttggcag tcttttactt
     9241 cattgattcc aatcattgtt atagtcactt ttacgcttat cggttcagcg gtttttcaac
     9301 agttgttcag aatgactgtt gttcaaggag tatggacata tgtccagctt cccattcaat
     9361 cgttcataga cacgttgttt ggtgtgcttt tattaggttt ttttgttccg tttttatggt
     9421 tatttggtct tcatggatct gcgatggtca atgggttggt tagtcccgtc ttgcaagcga
     9481 attcattagc gaatgcagaa atcttagcct caggacaaga gttgacggtg gcaaatggcg
     9541 gacatatcgt tactcagcaa tttctcgatc agttcatgac ggttacgggt gctggattga
     9601 cgttaggggc tgtatttttt atgacggttt ttgcgaaatc gaagaaatac agagaattag
     9661 gaaatcttgc tcttttacca gcctttttca acatcaatga atcaattatt ttttccacac
     9721 cgatcgtgat gaacccgatg atggctgttc cttttatttt cgcaccggtc gtttcaggat
     9781 tgattaccta ttccgcttta tattttggat tcgttccttt atttacagca gtccaagtcc
     9841 cttggacaac accgccgatc ctctcgggtt tcttagtagg cggagtgcct gcagcgattt
     9901 tacaaggtat cgtgttgtca attagttttt tcatttattt cccatttttg aaaaaaatag
     9961 attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga tggagcagca gaaaagatga
    10021 acgactaaaa aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc aatctaggat
    10081 gacaggagta tttatagtca tgtttatact agcccttttt ggacagctaa ttgaacaaga
    10141 acaactaatg tgttcattcc tacgtgtgtg atcatcgatg tccagatccg accagttgct
    10201 ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg accatcatta
    10261 tgagcaaacg caaacagcaa agagctgatc actgctccga cccaaaaatt tgaatatcga
    10321 ctgatgatcc ccagtatact gcggcggaat acgaactctt ccatgatggg tccagcaaca
    10381 gttgtcgcca aaataaaagc gggtgcttcc atgatcatct ggatgatatt ttgtgtattc
    10441 tcggaagggg ttgctttgcc aaacaataca gattctaaca tgacagcgat gctttgcaga
    10501 atcaatgcga caaagattcc aaccaatcca taaataacaa tctttggagc agaagctttt
    10561 tttaccgctg attcgatcgc taagggttca cgttgcttaa aataaaaaat agctaggaca
    10621 acggcgccta gtatatacat gactgcagtt gctgtgatca attcagacgt agattttaca
    10681 agtcctatcg aagctaggat caagggtgag ataaaaatta gaccatagca aaaaatgctg
    10741 aggaagctat atttttttaa agacatgtaa ttcctccttt taagggataa caagcatgca
    10801 aggcaagctt atatcgattg atttcaatta tatcataatt tcaataagca gctgtcagaa
    10861 taaaatttct tttctttgat ttaaaagctt gcaaaaaaga gggagaatga gtattattaa
    10921 aaatgtaggt tagcactcga aggttaagag tgctaacaga ttaaaaaaag agttatcgga
    10981 gggatttatc gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga
    11041 agaaaaaact gtcggaggaa tcgttttagc ttctgctgct aaagagaaac cccaaactgg
    11101 gacagttgtt gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc
    11161 tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag taaaatacga
    11221 aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg gcgattgttg agtaaatttg
    11281 gaagataaaa aaataatcaa aaataatcaa tgaggtgaat caatcatggc aaaagaattg
    11341 aaatttgcag aagacgcacg cgcagctatg ttacgcgggg tagataaatt agcagataca
    11401 gtgaaagtaa cattaggacc taaaggccga aatgtcgtac tagaaaaatc ttacggctct
    11461 ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt
    11521 gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga tattgcagga
    11581 gacggtacaa cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac
    11641 gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc aacaaaagca
    11701 gcagtcgaag aattgcataa tatctcaact gttgttgatt caaaagaagc cattgcacaa
    11761 gtagcagctg tttcttcggg ttctgataaa gttggacatt tgattgctga tgcaatggaa
    11821 aaagttggaa acgacggtgt catcacaatc gaagaatcaa aagggatcga aacagaacta
    11881 gatgttgtag aaggtatgca gtttgatcgt ggttacttat cacaatatat ggtaacagat
    11941 aacgacaaaa tggaagctgt tctagaaaac ccatatatct tgatcacaga caaaaaaatc
    12001 tctaacatcc aagatattct gccattgtta gaacaaatct tgcaacaatc acgtccattg
    12061 ttgattattg ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc
    12121 cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca
    12181 atgcttgaag atatcgctat tttgacaggt ggtacagtaa ttactgacga tctaggttta
    12241 gaattgaaag atgtaacaat cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa
    12301 gacaatacaa cgatcgtaga aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa
    12361 ttgatcaaaa accaaattgc tgaaacaact tctgattttg accgcgaaaa attacaagaa
    12421 cgcttagcta aattagcagg tggtgtagct gttgttaaag ttggtgcagc tactgaaaca
    12481 gaattgaaag aattaaaatt acgcattgaa gatgcattga atgctacacg tgccgcagta
    12541 gaagaaggaa tggtctcagg tggtggtaca gctctagtga acgttatcag caaagtttct
    12601 gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa
    12661 gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat tgttgataaa
    12721 ttgaagaacg tggaactagg cactggtttc aatgctgcta ctggagaatg ggtaaacatg
    12781 gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca aaatgccgct
    12841 tccgtttctg ccttgttatt aacgacagaa gcagtcgttg cagataaacc agaaccagca
    12901 gcgccagcag ctcctgcaat ggatccatca atgatgggcg gtatgatgta agaaaaagca
    12961 acttgcttat ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag
    13021 cgtttaactc caagaaataa gaagaaattc acggaaattg cttttcaaat ttttgtgaat
    13081 ttcagcttat ttccgaagga gttgcttctg cttccgctgt ttatacgtgt ttagagcgtg
    13141 agacaaaagt gttctttact tttgtcccac gctctttttt gtgtgtaatt ataatgaaaa
    13201 tagttatttt taatagttat aatattttat ctattaaaag taaatatcca ttca
//
LOCUS       DAZUKA010000080        13112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000080 DAZUKA010000000
VERSION     DAZUKA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.081"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..151)
                     /locus_tag="ACG8PN_001831"
     CDS             complement(<1..151)
                     /locus_tag="ACG8PN_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFS4861971.1"
                     /translation="MILIVKYRVEVLFMVTRIFILDDSLVFEKMLVEILEESRILLSP
                     WKFEIV"
     gene            complement(360..1196)
                     /locus_tag="ACG8PN_001832"
     CDS             complement(360..1196)
                     /locus_tag="ACG8PN_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4861972.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1207..2136)
                     /locus_tag="ACG8PN_001833"
     CDS             complement(1207..2136)
                     /locus_tag="ACG8PN_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4861973.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2328..2681
                     /locus_tag="ACG8PN_001834"
     CDS             2328..2681
                     /locus_tag="ACG8PN_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4861974.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2755..3411)
                     /locus_tag="ACG8PN_001835"
     CDS             complement(2755..3411)
                     /locus_tag="ACG8PN_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4861975.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3637..5571
                     /locus_tag="ACG8PN_001836"
     CDS             3637..5571
                     /locus_tag="ACG8PN_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4861976.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5657..6880)
                     /locus_tag="ACG8PN_001837"
     CDS             complement(5657..6880)
                     /locus_tag="ACG8PN_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4861977.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6874..8745)
                     /locus_tag="ACG8PN_001838"
     CDS             complement(6874..8745)
                     /locus_tag="ACG8PN_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4861978.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(8766..9884)
                     /locus_tag="ACG8PN_001839"
     CDS             complement(8766..9884)
                     /locus_tag="ACG8PN_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4861979.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9967..10800)
                     /locus_tag="ACG8PN_001840"
     CDS             complement(9967..10800)
                     /locus_tag="ACG8PN_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861980.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10793..11710)
                     /locus_tag="ACG8PN_001841"
     CDS             complement(10793..11710)
                     /locus_tag="ACG8PN_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4861981.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11937..13061)
                     /locus_tag="ACG8PN_001842"
     CDS             complement(11937..13061)
                     /locus_tag="ACG8PN_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4861982.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4100 a         2640 c         2165 g         4207 t
ORIGIN      
        1 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
       61 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
      121 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
      181 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
      241 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
      301 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
      361 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
      421 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
      481 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
      541 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
      601 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
      661 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
      721 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
      781 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
      841 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
      901 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
      961 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
     1021 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
     1081 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
     1141 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
     1201 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
     1261 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
     1321 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
     1381 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
     1441 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
     1501 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
     1561 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
     1621 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
     1681 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
     1741 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
     1801 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
     1861 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
     1921 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
     1981 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
     2041 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
     2101 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
     2161 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
     2221 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
     2281 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
     2341 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
     2401 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
     2461 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
     2521 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
     2581 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
     2641 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
     2701 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
     2761 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
     2821 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
     2881 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
     2941 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
     3001 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
     3061 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
     3121 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
     3181 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
     3241 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
     3301 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
     3361 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
     3421 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
     3481 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
     3541 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
     3601 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
     3661 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
     3721 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
     3781 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
     3841 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
     3901 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
     3961 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
     4021 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
     4081 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
     4141 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
     4201 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
     4261 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
     4321 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
     4381 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
     4441 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
     4501 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
     4561 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
     4621 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
     4681 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
     4741 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
     4801 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
     4861 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
     4921 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
     4981 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
     5041 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
     5101 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
     5161 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
     5221 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
     5281 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
     5341 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
     5401 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
     5461 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
     5521 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
     5581 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
     5641 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
     5701 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
     5761 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
     5821 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
     5881 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
     5941 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
     6001 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
     6061 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
     6121 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
     6181 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
     6241 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
     6301 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
     6361 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
     6421 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
     6481 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
     6541 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
     6601 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
     6661 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
     6721 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
     6781 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
     6841 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
     6901 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
     6961 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
     7021 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
     7081 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
     7141 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
     7201 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
     7261 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
     7321 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
     7381 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
     7441 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
     7501 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
     7561 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
     7621 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
     7681 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
     7741 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
     7801 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
     7861 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
     7921 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
     7981 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
     8041 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
     8101 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
     8161 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
     8221 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
     8281 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
     8341 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
     8401 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
     8461 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
     8521 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
     8581 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
     8641 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
     8701 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
     8761 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
     8821 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
     8881 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
     8941 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
     9001 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
     9061 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
     9121 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
     9181 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
     9241 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
     9301 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
     9361 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
     9421 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
     9481 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
     9541 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
     9601 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
     9661 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
     9721 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
     9781 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
     9841 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
     9901 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
     9961 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    10021 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    10081 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    10141 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    10201 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    10261 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    10321 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    10381 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    10441 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    10501 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    10561 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    10621 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    10681 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    10741 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    10801 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    10861 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    10921 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    10981 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    11041 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    11101 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    11161 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    11221 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    11281 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    11341 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    11401 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    11461 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    11521 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    11581 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    11641 tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag
    11701 attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac
    11761 ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa
    11821 tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca
    11881 gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg tcctccttag
    11941 tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg
    12001 cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt
    12061 tgtgccactt gtggattttt tgcatcttta gggacataaa aacttgatcc tcctgatact
    12121 gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca
    12181 gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt
    12241 gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag
    12301 tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata
    12361 tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct
    12421 tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt
    12481 gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca
    12541 aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc
    12601 gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca
    12661 tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc
    12721 acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc
    12781 aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag
    12841 tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc
    12901 agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg
    12961 atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta
    13021 atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt
    13081 tcaagttctt atataaaacg ctttcactta tg
//
LOCUS       DAZUKA010000081        13086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000081 DAZUKA010000000
VERSION     DAZUKA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.082"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG8PN_001843"
     CDS             complement(79..2199)
                     /locus_tag="ACG8PN_001843"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4861983.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG8PN_001844"
     CDS             2429..3607
                     /locus_tag="ACG8PN_001844"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4861984.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8PN_001845"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8PN_001845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4861985.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8PN_001846"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8PN_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4861986.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG8PN_001847"
     CDS             complement(4960..6213)
                     /locus_tag="ACG8PN_001847"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4861987.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8PN_001848"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8PN_001848"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4861988.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG8PN_001849"
     CDS             7790..9136
                     /locus_tag="ACG8PN_001849"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4861989.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG8PN_001850"
     CDS             complement(9444..9830)
                     /locus_tag="ACG8PN_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4861990.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG8PN_001851"
     CDS             complement(10098..10247)
                     /locus_tag="ACG8PN_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861991.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG8PN_001852"
     CDS             10709..10921
                     /locus_tag="ACG8PN_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4861992.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG8PN_001853"
     CDS             complement(11199..12998)
                     /locus_tag="ACG8PN_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4861993.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZUKA010000082        12918 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000082 DAZUKA010000000
VERSION     DAZUKA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.083"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..3644
                     /locus_tag="ACG8PN_001854"
     CDS             117..3644
                     /locus_tag="ACG8PN_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4861994.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3641..7363
                     /gene="addA"
                     /locus_tag="ACG8PN_001855"
     CDS             3641..7363
                     /gene="addA"
                     /locus_tag="ACG8PN_001855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4861995.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7432..7773
                     /locus_tag="ACG8PN_001856"
     CDS             7432..7773
                     /locus_tag="ACG8PN_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4861996.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8082..9125
                     /gene="pheS"
                     /locus_tag="ACG8PN_001857"
     CDS             8082..9125
                     /gene="pheS"
                     /locus_tag="ACG8PN_001857"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4861997.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9130..11550
                     /gene="pheT"
                     /locus_tag="ACG8PN_001858"
     CDS             9130..11550
                     /gene="pheT"
                     /locus_tag="ACG8PN_001858"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4861998.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="ACG8PN_001859"
     CDS             complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="ACG8PN_001859"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4861999.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4393 a         2245 c         2738 g         3542 t
ORIGIN      
        1 aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag aacaaatgtt
       61 cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg aaaagcatgt
      121 ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg atcgacgaag
      181 catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt cccaactaca
      241 gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc ggaaaaaaaa
      301 ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg tatttgctgc
      361 aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca atgatcttgc
      421 gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa atcaataaaa
      481 gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga aatattcaac
      541 cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag caacttaaaa
      601 tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa cgtgcactaa
      661 caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat ttcaacaacg
      721 tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa ttactgcagg
      781 caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct tatacagcgg
      841 atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc cgtctttttc
      901 aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca gagcagactc
      961 gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc caaaagaaag
     1021 cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct gaaaatccaa
     1081 ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa gaaaactacc
     1141 gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat atccttcggc
     1201 cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg atggaaaatc
     1261 atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct tatcggctaa
     1321 gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca gaaaccgaac
     1381 ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc actgaaaatg
     1441 tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac tggcagtttg
     1501 tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa gaacagtcaa
     1561 atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa ataaagaaaa
     1621 gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa agtggcgtac
     1681 agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta gaagcggcaa
     1741 gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat gtagtaattt
     1801 atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt ggtctagaaa
     1861 atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat cgtttggaat
     1921 tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat caagtgtttc
     1981 cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta ttaaatcagg
     2041 ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct tttgagcctt
     2101 tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt tatgcacaaa
     2161 gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc acagaatatt
     2221 tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct aattgttatg
     2281 tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta gctttagatg
     2341 aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg aattcgtcat
     2401 ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt cctgtttcga
     2461 tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca atttcacgta
     2521 tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg aaattgaaag
     2581 aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat gaatcattgg
     2641 atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg tccgatgcgc
     2701 agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt cggtttgaga
     2761 tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag acgattcaaa
     2821 aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct cttcagacag
     2881 agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa ttaccgttga
     2941 gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta gcttctgaac
     3001 aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca ttcgatgtca
     3061 cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta gcattgatgg
     3121 atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat tatttacatg
     3181 ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg aaaaaattta
     3241 gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat cattcactgg
     3301 aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat gaataccaaa
     3361 aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta ttcatggcgc
     3421 ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa atcaaactaa
     3481 atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc agaagcatct
     3541 gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa atagataagg
     3601 aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa ttcctttaaa
     3661 accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc aaggagataa
     3721 tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc gaagagtgat
     3781 tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat ttactgaagc
     3841 tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg tcaatagcga
     3901 aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc ccacagcgaa
     3961 tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt acttaattga
     4021 tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga tgaaggagga
     4081 tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat ttttccaatt
     4141 aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg tgttttctct
     4201 ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt taagcgataa
     4261 ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa tccgtccttt
     4321 agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc agttagcaca
     4381 aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc agatcaagaa
     4441 tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt tggaacaact
     4501 aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt ccaatgaagt
     4561 caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa gttattttcc
     4621 tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag tcgaagaaat
     4681 gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg aaaaaggtgt
     4741 gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa aaacgaaaga
     4801 tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag aagtaatggt
     4861 agatgaatat caagacgtga accaattaca agaagccatt ttatactggc taagagaacc
     4921 cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca tctattcttt
     4981 ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa agaaagaagg
     5041 cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc tttcttttac
     5101 gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt atgacgaagc
     5161 agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg agccggaaat
     5221 catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg atgatatact
     5281 ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc aattaattga
     5341 ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag agtacaaaga
     5401 cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga ttttcaaaac
     5461 attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga cagaaattcg
     5521 cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc cgcttgcggc
     5581 tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca ttcgattagc
     5641 taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa acgaagatga
     5701 attagcagct aagctggaac attttggcaa gcaactagag cattggcgag aacttgctcg
     5761 ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat atctagagta
     5821 tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat tggttgatcg
     5881 tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc gtttcattga
     5941 aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag aagataatgc
     6001 tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg tatttttgtt
     6061 agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt ttgaagaaaa
     6121 acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg atacgttacc
     6181 gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa tgcgtaaatt
     6241 gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct ataaaaataa
     6301 agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag tttttgatcc
     6361 tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg gattgatccg
     6421 tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc ttcaacattc
     6481 aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac aaagacagtt
     6541 gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg aaactttatt
     6601 agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt cttcacaaac
     6661 cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag atgatacaca
     6721 agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc aatttcgtta
     6781 tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag tgtcagcagc
     6841 tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga tgccaactac
     6901 agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag acgaaaaagt
     6961 cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac ttggtcaaca
     7021 gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc tgcttcctgc
     7081 tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc acggaatcgt
     7141 cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa cagattttat
     7201 tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc aaggacagct
     7261 aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa atgttttcct
     7321 aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat tgagcaaact
     7381 tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat gatggagcta
     7441 acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat tttaggaaaa
     7501 aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg ctttgttgag
     7561 cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt gaaggaatta
     7621 gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg ttcagaatat
     7681 ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca agtttggagc
     7741 gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct tgtaaactat
     7801 agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta aaccagttca
     7861 gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt tcactttccg
     7921 agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt gcggtgtaag
     7981 tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca ggaatgaggt
     8041 ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta caagcacaat
     8101 tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat ttaaatgaat
     8161 taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa gtattacgtg
     8221 ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca aatgagatcc
     8281 gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa gaagcgttaa
     8341 atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa gtaaaacatg
     8401 gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt gtgggtatgg
     8461 gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc gaacggatga
     8521 atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt tcagacgaga
     8581 tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa catgatttct
     8641 ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat acagatgatg
     8701 cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa aacatcacaa
     8761 tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg gaagatcgtg
     8821 aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa gtagatgtca
     8881 gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga tggatcgaaa
     8941 ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc gatccagaag
     9001 aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg cggtatggag
     9061 tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag ttcaaggggg
     9121 agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt atcgaatgta
     9181 acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga gggagtaacg
     9241 gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg cgtaccacat
     9301 ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga actgtctcaa
     9361 atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc acttcctaac
     9421 tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca agtatcaaat
     9481 ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc gaaagaatac
     9541 gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt ttttccttat
     9601 ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc ggatgcattg
     9661 tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc tacattcgat
     9721 gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt ggaagttgaa
     9781 gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag aatccagcca
     9841 agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat caacaatgta
     9901 gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc atttgactac
     9961 gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga aacattagta
    10021 acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac taacggcaaa
    10081 gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac gaatgaaaca
    10141 acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg tacagcaaaa
    10201 caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa ccatgcgact
    10261 gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg tggggaagtt
    10321 ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt gaagatttca
    10381 cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt aaatgagatt
    10441 tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt agtagtacca
    10501 ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc tcgtatttat
    10561 ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg aagcttgtca
    10621 aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg attgagcgaa
    10681 gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat aaatgaaagt
    10741 aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg aatgaatttg
    10801 atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac agagattgct
    10861 ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca tctgccgcaa
    10921 gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga ttggcagacg
    10981 aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt atttgaacaa
    11041 ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga aatgcatcct
    11101 ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg tcaagtgcat
    11161 ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt tgatttatct
    11221 gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa attcccagca
    11281 gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga attaacagaa
    11341 gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga tgtctatcaa
    11401 ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt cgtcaaccca
    11461 gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga aaaagcgttg
    11521 gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta acgatcaagt
    11581 agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca catctctttt
    11641 tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct ttacagcctg
    11701 cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat tttacttcag
    11761 aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag atttccataa
    11821 cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca aattcagctt
    11881 ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg cggcggtaat
    11941 gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag taggtgaaga
    12001 ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga tcagtataca
    12061 tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata ccgtttccta
    12121 acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt ccttttggtg
    12181 ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag ctttgtacga
    12241 tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca agattcgctt
    12301 tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct gtaggcacgc
    12361 tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc cgaacgcgtc
    12421 ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga atttgtgatt
    12481 ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct aaagcaactt
    12541 gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt gcttgcatgt
    12601 cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact cgggttttta
    12661 gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc gttaaaataa
    12721 tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa tatcttaccg
    12781 tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg ataaaataac
    12841 taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag aaactttaca
    12901 aattactaaa ctatcaga
//
LOCUS       DAZUKA010000083        12845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000083 DAZUKA010000000
VERSION     DAZUKA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.086"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..653)
                     /locus_tag="ACG8PN_001860"
     CDS             complement(<1..653)
                     /locus_tag="ACG8PN_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4862000.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     "
     gene            complement(703..2199)
                     /locus_tag="ACG8PN_001861"
     CDS             complement(703..2199)
                     /locus_tag="ACG8PN_001861"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4862001.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2393..4015)
                     /locus_tag="ACG8PN_001862"
     CDS             complement(2393..4015)
                     /locus_tag="ACG8PN_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4862002.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4343..5656)
                     /locus_tag="ACG8PN_001863"
     CDS             complement(4343..5656)
                     /locus_tag="ACG8PN_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4862003.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(5901..6125)
                     /locus_tag="ACG8PN_001864"
     CDS             complement(5901..6125)
                     /locus_tag="ACG8PN_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862004.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(6145..6567)
                     /locus_tag="ACG8PN_001865"
     CDS             complement(6145..6567)
                     /locus_tag="ACG8PN_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4862005.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(6709..7419)
                     /locus_tag="ACG8PN_001866"
     CDS             complement(6709..7419)
                     /locus_tag="ACG8PN_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4862006.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(7437..7934)
                     /locus_tag="ACG8PN_001867"
     CDS             complement(7437..7934)
                     /locus_tag="ACG8PN_001867"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862007.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(7971..8423)
                     /locus_tag="ACG8PN_001868"
     CDS             complement(7971..8423)
                     /locus_tag="ACG8PN_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4862008.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(8558..9163)
                     /locus_tag="ACG8PN_001869"
     CDS             complement(8558..9163)
                     /locus_tag="ACG8PN_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4862009.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(9613..10233)
                     /locus_tag="ACG8PN_001870"
     CDS             complement(9613..10233)
                     /locus_tag="ACG8PN_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862010.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(10235..11104)
                     /locus_tag="ACG8PN_001871"
     CDS             complement(10235..11104)
                     /locus_tag="ACG8PN_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4862011.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(11127..11534)
                     /locus_tag="ACG8PN_001872"
     CDS             complement(11127..11534)
                     /locus_tag="ACG8PN_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4862012.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(11553..12842)
                     /locus_tag="ACG8PN_001873"
     CDS             complement(11553..12842)
                     /locus_tag="ACG8PN_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4862013.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         3689 a         2737 c         2107 g         4312 t
ORIGIN      
        1 cctgaaacaa tataattata tgatataaga acctctttta atgtcttttg gacagtcggc
       61 atgatattgg aaaaaattgt actgatttgt tgaggaaacc ttgcgtgttg gatcaccgtt
      121 tctgttccaa caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt
      181 ccttttgtcg acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc
      241 gtttttattt gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc
      301 atactgacat accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc
      361 atgctggaat agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat
      421 tcaattgatt ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg
      481 tagatagcaa gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct
      541 tcagctaatt cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg
      601 agttgtcgtt tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa
      661 aaacctctca aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct
      721 atattcaagg tttttaccat tagactggat aatttgttta taatattcaa aactatcttt
      781 tttcaatctc ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata
      841 tcgcttcctc atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta
      901 accaatgatt tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag
      961 atactcacaa cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc
     1021 aatgccattt tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac
     1081 ataacgaaac cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa
     1141 aaagggatta tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt
     1201 atagacagcg ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca
     1261 ctgttcgtct ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat
     1321 ataagctgga taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa
     1381 ttgcagtgaa ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga
     1441 aaagctacac ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc
     1501 acgggcgttc gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt
     1561 agcaatgggt tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg
     1621 catacgaaaa gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta
     1681 gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca
     1741 ccccccatac tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg
     1801 ctcgatatca tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc
     1861 atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa
     1921 agcttttaaa cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc
     1981 aatcgcttca tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt
     2041 ttcttcattc cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac
     2101 attaggtaat ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc
     2161 gccccataaa aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca
     2221 acaatttctt acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg
     2281 cagaagagaa gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat
     2341 acataagacg atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta
     2401 cttccaacac aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca
     2461 gaggataatt tgacacaata acccttaaag cttcacgtgg aatagtgaac gtctgctttt
     2521 cactggacat attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa
     2581 agacatgctc aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt
     2641 ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg
     2701 cttcttctgc attgatttct ttataatttg gattgactcg aagccatgga attccagtag
     2761 taaacccagc atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt
     2821 tcgccttgat tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat
     2881 acatattgat tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca
     2941 tgcctaactc ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag
     3001 caaacatctt ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc
     3061 gcggaagatc gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt
     3121 gccatttgga taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt
     3181 ctttgccttc ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat
     3241 ttcttttagg attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa
     3301 cagtcatcag atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca
     3361 gtttaggtcc attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt
     3421 ccatacgaaa gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt
     3481 ggcgaacctg tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa
     3541 aatattgccc cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc
     3601 tattaggctc ttttccattg accggatcac gccaaatata atagtcccga taaggatttt
     3661 ctttattttt acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca
     3721 gatccatgat gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt
     3781 ccatcgtacc gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat
     3841 cattcggaga ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat
     3901 aatccaacct ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa
     3961 aacttcttgg atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt
     4021 tcctcctata aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact
     4081 aaaaattgta acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa
     4141 aaaaacagct gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag
     4201 aatgctgatt tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc
     4261 tcaaaaaacg gcaaaatata agaaactatt ttttcgtttt tttctctaat actcaggcca
     4321 gaacgctgat ttcacaacct ctttatttca cattcaatat ccacatgaat cccacaaata
     4381 caaagaacag accatacatg attggagata cttcttttcc tcttttagct gctaacatgg
     4441 taattggata gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca
     4501 tgccgatcaa gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc
     4561 gcaaagattg tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag
     4621 taacaactga tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa
     4681 tagctgtgat acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac
     4741 caacaggaga agtaccaaat aaggaacccg caagcatagc tgttgaatca gaagctaaag
     4801 ctttccctac acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa
     4861 ctaaagttcc tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca
     4921 tttgaagaga attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac
     4981 ttggtgctgc ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa
     5041 tcgttgtagc tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag
     5101 cagttacgac taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc
     5161 ctactaatgt agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcga
     5221 taaatagacc gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga
     5281 tcatttcccg cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc
     5341 cggctaatgc tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag
     5401 cattgatccc aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa
     5461 cacagccgat cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag
     5521 aagcacctaa tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa
     5581 atcctgccag tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa
     5641 gtattttttc cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa
     5701 aatttcgatt tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa
     5761 acaaaaaatt cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct
     5821 caaagttcat gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt
     5881 cttcattcta gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct
     5941 tctttgatga attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta
     6001 tcgaccattt ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga
     6061 agcttctcac tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga
     6121 tccactgttt ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata
     6181 attgccttca gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc
     6241 tggttcttcc atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg
     6301 cggtggtcct aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa
     6361 ttctaattct tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt
     6421 ttcagaaatt tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt
     6481 gacataaagc atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt
     6541 tctttcctgt ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga
     6601 tactattcaa gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt
     6661 tacaaaaaaa aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc
     6721 ttctgattca tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat
     6781 tttttccttc aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga
     6841 ttcaatgacc agcaccgctg cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg
     6901 ccagttgaga cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag
     6961 tgccggagca tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc
     7021 taaatgcaga tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga
     7081 caaggaaata ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg
     7141 atttgcacgt ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt
     7201 ggtaaatgcc tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag
     7261 gtaatttctt gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc
     7321 ttttgttacg tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa
     7381 tacttcccag aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag
     7441 aataattgct tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt
     7501 tcgctgtatt tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc
     7561 caaacaccga gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct
     7621 agtaaaaatg taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt
     7681 tctaaaaaat gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa
     7741 cccgcaggct cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct
     7801 tctgtaagtt tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa
     7861 ttatcttttc caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca
     7921 tctgaaggcg tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc
     7981 cttttattgc cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt
     8041 aaaagatatt ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg
     8101 gagtagttgt tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag
     8161 agtttcttga tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct
     8221 aatttcttga ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt
     8281 tcttggatga ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt
     8341 aaatcgactt ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg
     8401 atgtctcgaa ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttca
     8461 cctctttttg ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac
     8521 tagtaaagta tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt
     8581 tgttttctaa aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact
     8641 cttctatact aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt
     8701 caccgtggat cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt
     8761 tttgtttatt cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt
     8821 cttcatagac ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac
     8881 caggaagtga ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt
     8941 tgatataggc acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg
     9001 cttttatttc ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc
     9061 ggatctcctc aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag
     9121 aaagtgcgag tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt
     9181 tagcaaatgt atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat
     9241 ataatgaaat ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga
     9301 cttctctggg gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca
     9361 ccaaacgaaa aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac
     9421 agctttttca caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc
     9481 acaatattca tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct
     9541 gaacgacagg ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa
     9601 gccatttctt tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt
     9661 gtaatccact tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt
     9721 gtcatatttt cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc
     9781 tttcgttcga tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg
     9841 acgatctgat tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt
     9901 taggcgaatc gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt
     9961 ttgcttatca ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa
    10021 atcaagtgct ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc
    10081 caaattgtta aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc
    10141 atacatatct ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg
    10201 actaccagcc agaagaatga aatacttttt catctcattt atttcctaag ttaattggac
    10261 tttccaagtt cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg
    10321 ctggaatcac attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag
    10381 ctactttcgt ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat
    10441 tgatcaattc aggataatct tcttctttca cacctgttcc tccatggata actaggggga
    10501 catcaactac tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga
    10561 attgtccatg attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa
    10621 atttcactgc gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt
    10681 cttctgtacc gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat
    10741 attctactac tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat
    10801 acatgacaga agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt
    10861 ggtctaagtg caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga
    10921 ctaacacatg catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg
    10981 aacccatttc ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa
    11041 aagctccaac tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat
    11101 acatctttta ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca
    11161 atttgtcgca tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca
    11221 cgagcagcat ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg
    11281 atcgtctgtt tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac
    11341 aattcagaaa atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg
    11401 gtagtggaaa tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct
    11461 ggcgataggg ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta
    11521 aaaaatccaa acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag
    11581 atccaggcat ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgttg
    11641 aatcccatcg aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca
    11701 tgagcaatac cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct
    11761 gctgtccctc cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac
    11821 atgggcaaag taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg
    11881 acagcatttg ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga
    11941 actaatttca tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca
    12001 ccagctagca atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg
    12061 atatagtttt gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg
    12121 atgatatata gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg
    12181 aaatgtgtcg gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa
    12241 agcttagcaa caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata
    12301 tcatttgatc cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg
    12361 aaacaagctc cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa
    12421 tagccaaaga ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg
    12481 tacttccatt ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc
    12541 agtgcaatat ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata
    12601 gaagggatga tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa
    12661 cttccaacga tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac
    12721 gtgccagaga tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc
    12781 aatgcaatgg tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc
    12841 atgtt
//
LOCUS       DAZUKA010000084        12606 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000084 DAZUKA010000000
VERSION     DAZUKA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.087"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACG8PN_001874"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACG8PN_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4862014.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACG8PN_001875"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACG8PN_001875"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4862015.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACG8PN_001876"
     CDS             complement(2298..2960)
                     /locus_tag="ACG8PN_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4862016.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACG8PN_001877"
     CDS             3144..4790
                     /locus_tag="ACG8PN_001877"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4862017.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACG8PN_001878"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACG8PN_001878"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4862018.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACG8PN_001879"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACG8PN_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACG8PN_001880"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACG8PN_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACG8PN_001881"
     CDS             complement(8939..10018)
                     /locus_tag="ACG8PN_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4862019.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACG8PN_001882"
     CDS             complement(10121..10561)
                     /locus_tag="ACG8PN_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4862020.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACG8PN_001883"
     CDS             complement(10618..11028)
                     /locus_tag="ACG8PN_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4862021.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACG8PN_001884"
     CDS             complement(11208..11642)
                     /locus_tag="ACG8PN_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4862022.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACG8PN_001885"
     CDS             complement(11742..12590)
                     /locus_tag="ACG8PN_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4862023.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4212 a         2347 c         2243 g         3804 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaa
//
LOCUS       DAZUKA010000085        12463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000085 DAZUKA010000000
VERSION     DAZUKA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.088"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1951
                     /locus_tag="ACG8PN_001886"
     CDS             68..1951
                     /locus_tag="ACG8PN_001886"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4862024.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2026..2310)
                     /locus_tag="ACG8PN_001887"
     CDS             complement(2026..2310)
                     /locus_tag="ACG8PN_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4862025.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2300..3034)
                     /locus_tag="ACG8PN_001888"
     CDS             complement(2300..3034)
                     /locus_tag="ACG8PN_001888"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4862026.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3163..3786
                     /locus_tag="ACG8PN_001889"
     CDS             3163..3786
                     /locus_tag="ACG8PN_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4862027.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3978..5099
                     /locus_tag="ACG8PN_001890"
     CDS             3978..5099
                     /locus_tag="ACG8PN_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4862028.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5106..8234
                     /locus_tag="ACG8PN_001891"
     CDS             5106..8234
                     /locus_tag="ACG8PN_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862029.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8253..11447
                     /locus_tag="ACG8PN_001892"
     CDS             8253..11447
                     /locus_tag="ACG8PN_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4862030.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11593..12300)
                     /locus_tag="ACG8PN_001893"
     CDS             complement(11593..12300)
                     /locus_tag="ACG8PN_001893"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4862031.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4241 a         2204 c         2629 g         3389 t
ORIGIN      
        1 cttgctattt tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga
       61 atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg
      121 gctccgttca ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa
      181 aggcggctat ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct
      241 gctgcggcag gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag
      301 aatgaagaga ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac
      361 tttgtttcag cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta
      421 tgtcaacaac tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga
      481 gtcaccgttt acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc
      541 gatcttgttt gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt
      601 gttaggtaca tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga
      661 tccaacccgt gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc
      721 gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa
      781 tattctgaat atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt
      841 agatgatcat tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt
      901 agtgctagtt gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc
      961 agtatttact tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat
     1021 gattttttat gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt
     1081 ggaaatcaca ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct
     1141 agcacgtttt gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat
     1201 catcagtaag agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt
     1261 tggtttcgtt acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa
     1321 aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag
     1381 ttcggttgac caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc
     1441 acaaaaatta gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct
     1501 acaagaactt tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag
     1561 tgtaccgtac attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgatcgg
     1621 actaggaacg aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg
     1681 tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt
     1741 gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta
     1801 cagctacagt aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga
     1861 agggatgaca cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac
     1921 gaacagcagt gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat
     1981 ttgtttttgc ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg
     2041 aagcaaaacg gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt
     2101 gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat
     2161 cttaacaggt cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc
     2221 ctctaatcgt ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa
     2281 taatacgtaa aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt
     2341 ctccatccat attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt
     2401 ctttgattcc ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt
     2461 ggatcctttt aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat
     2521 caggacgatg gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga
     2581 tcacttcttc taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt
     2641 tcgttttatt tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag
     2701 aattatgtcg aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt
     2761 catttaaatg gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt
     2821 cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc
     2881 ctgcacaaag gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca
     2941 cagcatctaa agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt
     3001 ataattggtc gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata
     3061 gtcgtctgta ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt
     3121 ttttatgcat actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg
     3181 cgcggggtag ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa
     3241 gaagttttac cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac
     3301 ccattatatc tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa
     3361 ctgttcaaaa atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa
     3421 agagaaaatc caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat
     3481 ttgagtttga tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg
     3541 gcattgattt ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg
     3601 acacttgggg atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac
     3661 ttatcagata tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa
     3721 gcagcttttg acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa
     3781 aaataaaatg ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca
     3841 gactttttca atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata
     3901 tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca
     3961 ttttttgagg tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac
     4021 tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg
     4081 cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc
     4141 cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa
     4201 agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta
     4261 cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac
     4321 cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga
     4381 gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag
     4441 ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg
     4501 taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga
     4561 tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag
     4621 ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg
     4681 agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt
     4741 ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc
     4801 tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg
     4861 atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg
     4921 ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa
     4981 tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa
     5041 ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat
     5101 caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg
     5161 tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg
     5221 gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac
     5281 ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga
     5341 ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt
     5401 tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt
     5461 ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga
     5521 tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag
     5581 gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt
     5641 tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac
     5701 agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt
     5761 tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc
     5821 atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg
     5881 aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg
     5941 aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag
     6001 aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc
     6061 agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa
     6121 caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc
     6181 gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga
     6241 tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt
     6301 cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg
     6361 aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc
     6421 ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag
     6481 caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa
     6541 taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc
     6601 aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc
     6661 ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg
     6721 aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc
     6781 agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt
     6841 tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac
     6901 aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag
     6961 taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg
     7021 aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc
     7081 agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac
     7141 aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt
     7201 tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag
     7261 atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg
     7321 aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt
     7381 caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac
     7441 gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag
     7501 aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag
     7561 aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa
     7621 ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat
     7681 tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga
     7741 agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa
     7801 atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg
     7861 gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt
     7921 ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat
     7981 tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta
     8041 ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag
     8101 aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag
     8161 aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct
     8221 atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca
     8281 ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag
     8341 aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg
     8401 gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta
     8461 agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa
     8521 aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag
     8581 gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat
     8641 accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt
     8701 acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg
     8761 attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc
     8821 gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa
     8881 gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata
     8941 aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga
     9001 ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc
     9061 aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat
     9121 tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt
     9181 attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat
     9241 tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta
     9301 tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag
     9361 cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc
     9421 gagtcgattt cgtctatggc gaagtcatct attcgactga cccgaagcat gaggtctcga
     9481 cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat
     9541 tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg
     9601 aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca
     9661 gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg
     9721 aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc
     9781 aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa
     9841 atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac
     9901 aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat
     9961 tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga
    10021 tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg
    10081 caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc
    10141 agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct
    10201 ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga
    10261 gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag
    10321 taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag
    10381 tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt
    10441 atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat
    10501 tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt
    10561 tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa
    10621 aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc
    10681 gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg
    10741 cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa
    10801 tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga
    10861 caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt
    10921 ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag
    10981 ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc
    11041 tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg
    11101 tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa
    11161 ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg
    11221 ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag
    11281 tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa
    11341 aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg
    11401 aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa
    11461 gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa
    11521 atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc
    11581 tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga
    11641 aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag
    11701 cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta
    11761 taaggtctct tctggaagca tatgaaattc tttctcagct agctgaaaaa tctctttttc
    11821 tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt
    11881 caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc
    11941 taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca
    12001 gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat
    12061 atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag
    12121 tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat
    12181 atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg
    12241 aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa
    12301 aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag
    12361 aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt
    12421 gacagaataa ggtttttata tttcagtttt tttcactaaa aat
//
LOCUS       DAZUKA010000086        12174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000086 DAZUKA010000000
VERSION     DAZUKA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.089"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG8PN_001894"
     CDS             <1..610
                     /locus_tag="ACG8PN_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4862032.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG8PN_001895"
     CDS             723..1001
                     /locus_tag="ACG8PN_001895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862033.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG8PN_001896"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG8PN_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4862034.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG8PN_001897"
     CDS             1458..2213
                     /locus_tag="ACG8PN_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4862035.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG8PN_001898"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG8PN_001898"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4862036.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG8PN_001899"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG8PN_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4862037.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG8PN_001900"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG8PN_001900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4862038.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG8PN_001901"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG8PN_001901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4862039.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG8PN_001902"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG8PN_001902"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4862040.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG8PN_001903"
     CDS             6950..7492
                     /locus_tag="ACG8PN_001903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4862041.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG8PN_001904"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG8PN_001904"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4862042.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG8PN_001905"
     CDS             9085..9987
                     /locus_tag="ACG8PN_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4862043.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG8PN_001906"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG8PN_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4862044.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG8PN_001907"
     CDS             11430..11852
                     /locus_tag="ACG8PN_001907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4862045.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4046 a         2159 c         2687 g         3282 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tctt
//
LOCUS       DAZUKA010000087        11784 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000087 DAZUKA010000000
VERSION     DAZUKA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.090"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..769)
                     /gene="truA"
                     /locus_tag="ACG8PN_001908"
     CDS             complement(29..769)
                     /gene="truA"
                     /locus_tag="ACG8PN_001908"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4862046.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(960..1595)
                     /locus_tag="ACG8PN_001909"
     CDS             complement(960..1595)
                     /locus_tag="ACG8PN_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4862047.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1742..2962)
                     /gene="sstT"
                     /locus_tag="ACG8PN_001910"
     CDS             complement(1742..2962)
                     /gene="sstT"
                     /locus_tag="ACG8PN_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4862048.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            3181..4179
                     /locus_tag="ACG8PN_001911"
     CDS             3181..4179
                     /locus_tag="ACG8PN_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862049.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4375..6210
                     /locus_tag="ACG8PN_001912"
     CDS             4375..6210
                     /locus_tag="ACG8PN_001912"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4862050.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6270..6986)
                     /locus_tag="ACG8PN_001913"
     CDS             complement(6270..6986)
                     /locus_tag="ACG8PN_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4862051.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7213..7755)
                     /locus_tag="ACG8PN_001914"
     CDS             complement(7213..7755)
                     /locus_tag="ACG8PN_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4862052.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7760..7987)
                     /locus_tag="ACG8PN_001915"
     CDS             complement(7760..7987)
                     /locus_tag="ACG8PN_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4862053.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8066..9946)
                     /locus_tag="ACG8PN_001916"
     CDS             complement(8066..9946)
                     /locus_tag="ACG8PN_001916"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4862054.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10135..10749)
                     /locus_tag="ACG8PN_001917"
     CDS             complement(10135..10749)
                     /locus_tag="ACG8PN_001917"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862055.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10891..11451
                     /locus_tag="ACG8PN_001918"
     CDS             10891..11451
                     /locus_tag="ACG8PN_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4862056.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3680 a         2354 c         1898 g         3852 t
ORIGIN      
        1 atgcgtttca tccattatca aagtattttt atcgataata aacttcatcc aaaaacagac
       61 cttgtgcagg gacggtttct cctgcatgtt gccgcacttt gctttcaaat atttcatcga
      121 ttgtttctat cggcatggca cctgtgccga tttctagtaa tgtccccatg ataatccgca
      181 ccattttata taagaatccg tctccgacaa aagtaaaatg aagcatttct ccctttcgtt
      241 cgatagataa ttcatcaatc gtacgcactg tggatttttt tgattttttc aatgcagaaa
      301 aaccaatgaa atcatgtgta cccactaatt tttcgcaagc tgcattcagc ttgtccatat
      361 ccaactcttg agggaaataa aagctgtggt ttcgctcaaa tgctgaagga atcacgttgt
      421 tccaaacata atagctgtat tttttgccca ccgcattata tcttgaatgg aagcgctcag
      481 gcatttcttc gattcttttg atgacaatat cccgcggaag gtaacgattc ataaagtcga
      541 tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt tgctacttgt cctcgcgcat
      601 gggtgccagc atctgttctt cctgatccaa taatttctat ctcttcattt gtcatctgat
      661 gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc atctccaagt ctttgccatc
      721 ctgaatatct ttttccgtca tattcgattg tcaacttgat gtttctcata ttttagtggc
      781 tcctctgtct tccatttggt cttctctcta cctttttaag attgtagcac aaaatatagc
      841 tccttgcttc tctaagacag aagctataca agaaaactgt ggaaatatat atttcagaaa
      901 aacaaaaaac agccaccgga tgaagtatcc gctgactgtt caatttgacg gctcaagttt
      961 tactgtttga gatgcaagat actcaaccag gcaatgggca cataattaaa gaaaaaactg
     1021 attctttggc aaaaacctat tttattcagt aatggccaag aatttataga aggaattctt
     1081 gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag acagtccaag gtataagtgg
     1141 cttacgtcca tttgcccatt ttttcgatag atcaagaaaa gaagaactgg aaacaataaa
     1201 aaaccactgt aacctaaacc tgatccgata ttatggaccc atgtcgagac attccacgaa
     1261 gattcatggg tattgatact gaacaaacct gtaaagatac aatcacctat accatagaac
     1321 gcgatggctg ctgtaactag ggtagctaac acacgagatg tactaacaaa tgcagcatac
     1381 aatgcaggca atgacaggat gaaaaagatc cctgaaacca cagaccaaat aagaaacgct
     1441 tttcttacag gactggaaac ctctccaaat acactgatca cgctcttcat ttgatctaat
     1501 tcaggataat acaatcctaa tacgtacgga gtcaaaaaat cactcagcac gcctaataat
     1561 aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac gcctccaata acaattacct
     1621 atgcttttaa ttgttattat aaacgacttt atcttgattt aacagaaacc tctatttaca
     1681 cgaacatcta tataactcat taaaaactcg aatcatttta aaaatactaa atatctaacg
     1741 ttcaacgttc acttaatcca tctaaacaat ctttcaattt gattttttcc ccacttcttt
     1801 tacgcgcgcc tagctctcct attgcagtaa acaacagatc acttgacgaa tttaaggcag
     1861 tttccattga atcctggata acaccgataa taaatccaat accgacgatt tgcatcgcga
     1921 catcattaga gataccaaac aaactacatg ctaatggaat caataacagt gaaccgcccg
     1981 caacacctga agctccacaa gcagctaaag cagacaaaat acataaaagg actttcaaaa
     2041 caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa cgtcatcaag gaaatggtaa
     2101 tcgcggcacc gcccatattg atcgtagcac ctaacgggat cgatatggaa taagactctt
     2161 ttgtcaattt caatctttca gccaacatca tgtttatcgg gatatttgcg gcagaacttc
     2221 gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa gaaaatcaat ggatagggat
     2281 tttctttaat aaacaaaaac gtgaggaatg ggtacacaac taatgcgaca aacagcatcg
     2341 taccgatcaa aacaagtaaa agctgcagat actctgccaa tccagcgatt ccagtcgtag
     2401 caattgattg ataaaccaat cctaaaatcc ctatcggagc aaatgcaata atcacttgta
     2461 cgacttgtga caacgctata gatatctgat ccaccaactc ttttgtcgat tctgaacgtg
     2521 ctcgaaaagc tagtcccaac aatacagccc agaataagac gccaaggtaa ttcgcatcaa
     2581 taagagattg aacaggattt tgtgtgacat tcgtcaacat agtagataaa acactttcca
     2641 aactttgcgg tgctttttca gatactgttt cttgtaaagt aataggaaca gtaaaaattg
     2701 aacttacaat aactgctaca agtgcagaaa gcaatgttcc cactaaatat aagatcaaaa
     2761 taggcttcac gaatgtctta ttcccaattt tatgcttggc aattgacgcc atcgtcaaga
     2821 aaaaaaccag aataggtgcg attgccttca agcaactgac aaacaacgtt cccaatattg
     2881 aaataaatgt ccatgtgggg aaaaagatac caaacaatat tccgactaaa ataccaacaa
     2941 caatacgttg aactaacgac atatccaaaa acttctctaa caaatgacca ccctttcaca
     3001 attttaacaa tcattgtatc attttatgca aagaaactaa aggtataatt ttctaaaaat
     3061 tctaacaata caaacaacca tttcttgcta attttgtact ttttctgtct acacgttgtt
     3121 ttttgagata ttctaagtta ggatgttatt aatgggcaac ttatcaggaa acgttttctt
     3181 atgtttttgt ccaatttttt tgtgaggtgt ttattcttga aaaaaataaa gaaggagagt
     3241 tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg ttttacagcg tatattgatt
     3301 tcagcagtca ctattcctac atatgcaaca aaccctggtc gattctacat ctatacattt
     3361 ttacaaactt ccgttgttat tggtgcaaat ttgatcccac tttacatggg ttaccaatat
     3421 caaaaaatcg cccgtttaaa agttttacat tatttaagtc gttttgcctt tatattccta
     3481 atagccacac tagtttgcaa tatctttttt tacgtacaag ctggcagctt gaatattcgt
     3541 gattattggc ttatcctaac acctatcagt cagaattact ttacttatgc tgtttcttgt
     3601 actttgcttt tttgtagtat tccttataca gttggatttt taaataaatt atcgaaaaca
     3661 acaattaaat atctactatt atttttaaca attggattag ttggctgctc tactctattt
     3721 aataaagacc cgtttgcatt acagaatgga cagagtattt tatggatttt ttatttattt
     3781 ttaatgggtt atggtttgaa agaatggaac tggaaagaaa aggtccgcta taagcttcct
     3841 caagtattta tcagcttgat tgttttattc ggtttgatta ttttgatgac acaaatttca
     3901 ctcatcatca gaggaaatac agatacagcg ctgaggtttt ctaaaccata ttctctcttc
     3961 gcaatgtatt atactttctc ttcgttttta tttttagaaa tgctatctga taaattaggt
     4021 ataaaaggaa gaggaaattt tcttgctgta tttttgattg tcactcaagt tatatgtaat
     4081 ttccctttag tttcttatcg cgtatctaca tttatgaaac gtgaatttcc aaactcaggg
     4141 aaggcatggc tgataaatat cgggcagttt tttatttgat atttattagc agtgtccctc
     4201 ctagttatct ttttatttta tatacaaaaa atccgtttca ttcatcgttt catcgaatac
     4261 ttcgcttttg attcgattga acaattaact cagcgttgga aaaaaagaat tcattggctt
     4321 tttgttcata aaagttattt tttagttgca gcattttttt actgctttac cttcgtgcag
     4381 attttcgttt tagaaccaaa agaaggttgg aaagaaacta tccaggttgc cttaaaaata
     4441 tttactcagc gacaagcacc catgatcctg acaatcatca ttattatggg atttttcttc
     4501 ttactattgt tgatttccaa tcgtttttgg tacgccttga ctgctacctt gatcattaac
     4561 ttactgctca ctatttcgac agtaatcaaa atggaaatgc gggaagagcc tgtctttcct
     4621 tcagatttaa aaatgctgac cggattgtca gaactattat cgatggttag cccagtctta
     4681 ttaatcgtgg gcggattgat ccttctccta ttattgatta ctagtattat tgtacaacgt
     4741 agactacaaa aacagtattc cttgaagatt cattggaaac gtcgatttat cagtatcgct
     4801 gtattattag gatgtttttc gggtgtcttt ttcattaatc acaaaaattc gccttctttc
     4861 ttgctgttta atttatttaa agtaaacaag actttcttta accagcaagg cgccgtaaaa
     4921 gataacggac cagtcattca attcctaaac aatatcgaca ttaaaatcat ggaagaacct
     4981 gctggctatt cgaaagaaaa aattaatgga ataatgaaaa agtacaacac aaaggcagat
     5041 gaaatcaacc aaacaagaag tgactggtta aacaatcaga cgatcgttct caatttaagt
     5101 gaaagcttca gtgatcctgc acgtgtccct aatctgacag tacctacaga ccctattcct
     5161 tacatcaaaa gcattataac agaaggaaca ggaggattga tgttatccgt cggttatggt
     5221 ggcggaacag ctaacatgga atgggaagga ctgacaggct tgagtatcag taacctgtct
     5281 gcttcgttag ttacacctta tacccagcta gtggagcgtc agaaaataag tccaaatatt
     5341 accgatctat tcgatgaaaa aatagccatc catcctttta cagctgctct ttatcgcaga
     5401 aaacaagtgt ttgataaatt tggttttgac aggttctatt atttagacag tccggataaa
     5461 ttaacttata cggataaaat agaacaaagc ccacgcatat cggatgattc agcatacaaa
     5521 gaaaccttga aagctttaca aagcaatacg gaaacgtcac aattcatcca attatccact
     5581 atgcaaaacc atatgcctta tgatgattac tatagtgaat tgaactacac tgctgaagga
     5641 actgctgtca ttcctaatag aagaaaagaa ttacaaactt atatgcaagg ccttcactat
     5701 accgatacag cagtaaaaaa attcatccaa gaaatcgaca aaatcgataa accgattacc
     5761 tttgtttttt atggtgacca tttacccgca atctatagtg ggaatgattc gaaaaaatat
     5821 ggattagaac agcaccagac agattatttc atttataaca acaagtatag tcgtgaacaa
     5881 gcagctacgt taaataaaga agttgttgct cctaataact ttggtgctat ggctcttgag
     5941 catgcgaata tcaaagtaac tccttactat gcgttgttga cagaagtatc taatcatcta
     6001 cctgcattaa caattgatcc tacagaaagc ttgtctaatc gttttaatgg aaaacaggtc
     6061 tttgttacag aggatagtca aattatccaa gaaaaaaatt taactgagaa acaaaaagaa
     6121 attctagaag attatcgtct gatacaatat gatcttgttg caggtaacca gtacagtgca
     6181 acatgggcga cacaaaaaat aaaagactaa caaataaact gagcaagggg ttgtgataaa
     6241 agacatgtca caaccccttg tttatctcat taatttgctt gtccataaac gatttgatta
     6301 aagccatttt tcaattcatt attcgctgtc tcgttgaagt aaagataatt atcgggattc
     6361 ccctgatttt gttgtgtcac caatactttt ggctgatttt gctgaaaccc gaatagatag
     6421 acgtgctttt tcagatatgg ttctgtctct gcatcggaat agacggctgt gatttgatag
     6481 tctgcttgac ctgtcccact ttctgaccat tctgcagaaa caggcactcc accaatagcc
     6541 atcttccact ccccgttcaa aatagcttga gggactttaa gaccatataa gtccacactc
     6601 atttgattcg tataactttt gtattgttgt cccattgttt ggccccattt ggtcataaat
     6661 gcctctaatt gtgcggattt gtctgcattc cacagtgttt gttcaacttg ttcgctgctt
     6721 tctgaagagg aagatgaagc ctctacagaa gaacttgaag tagatgtttg agttgtttca
     6781 gtcgttttgg aagactctga cgaagtagag gtatctgctg tttctgtact tgctgcagtt
     6841 gacttgctct ctgtgctagt tgttgtagta gttgcaactg ttgctttcgc tgtttctttt
     6901 gttgattctt ttttcgattc attcccgttg gtacaaccag ctaacaaaaa agctagacca
     6961 attgttacta aaatcctttt tttcaattaa aaaactcctt tcgattactc ccacttcttt
     7021 attctatcaa ataagcaatg tgattttatt gatttttcgt attttctcat caatctttca
     7081 aatcttctaa aaactgagaa gcacccgctg atgttgtctg taatacatca gcgggtgctt
     7141 cttttatttt aatcttcgtg attttccaat ttacctcacg ctttatattt gtatttgaag
     7201 aaacatagac ttttagtcat catcgtcttc ctcatccaaa tcttgtcgga cagtttttcc
     7261 ttggagtctt tgtacttggt agatgatttc tttattgaaa cttaaaagtt caacaagcaa
     7321 ttgttgcaag ccatattttt cttctttttc agctaatttg attgcccgtg tgataaagac
     7381 attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt gttgaggctg gtaaatattt
     7441 gttttgacca tgttcgtaaa tttttccata acgcatgact tcttctaaag tagacggaac
     7501 gatttcattt tcaatcacta actcatgaga gattttattg aaccatctcg cttcttgatc
     7561 agcatattct gaaaaaaggg tacttaattc ttgcgaagat gtacccaaaa catacaattg
     7621 tgcttggatc aatttcattt cttctaattt aaaatttgct aatacatgcc ccaccatggc
     7681 gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt gtttcttgaa caaatttttc
     7741 ttccactata ttcattgatt tacgcctctt ttacttttgc tgattggact tcataaccta
     7801 atttttccac aactgcagct aattgttcag ggtggacttc atctggatca aagtttgctt
     7861 tgattttgct cgcattaaac aaaactttcg tattgcttac cccaggctgt tgagaaaccg
     7921 catgttcgat tttttgcata cagcttggac aagctagtgt atctaattgc ataataattt
     7981 ttttcatcgt agttcttcct tcctttttct tgatagtctt attctagatt acagcttcca
     8041 taaaaaaatt gattcttatc aagttttacg atttttcttc tttgctttcg tgtccaaaat
     8101 acaataaacg catcgcgtta aagataacga tcaagatact agcttcgtga acgaacatcc
     8161 cactagccat gtaaataaac ccggcaaata agccgttgag taaaaataag acgacaccaa
     8221 tggcaatcac tacattttca cgggtattta aaacagtttt cttagccaag cggtaggcat
     8281 gaaccaacga ttcaaaatta gattgcatca agacaacgtc actcgtttcg atagcaacat
     8341 ccgttcctga tcccatagca atcccgatat cagccgcagc caaagaggga gaatcattga
     8401 ttccatctcc aataaaagca acgcgcaatc cttgttcttg gtattttttc acaaattgaa
     8461 ccttttcgtc tggtaataat tcagcatgga cttcatcaat gcctaacata tcagctacat
     8521 aatcagcagt catctgatta tcacctgtca gcatcactaa ctgttttgct cctgcttgtt
     8581 tcaacttagc taattgttca gcgacttctg gtcgaatcac atctgacaca cctatgattt
     8641 gagtgatttt tttgtcgatt gctacaataa tgaccgagct gcctgtttgt tgtaattgat
     8701 gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg agcggcaatc aatttggtgt
     8761 tgcccgcaca aactgtatga ccatcaatct cagctgtgat tccttgtccc ttgattgtct
     8821 gattatcttt gactgctaat tgttgaaaat caatgtcttt ctcctgagca taatggatca
     8881 cggcgcgccc cagaggatga tcggataacg tttctaatcg agccactaaa gctaagctgt
     8941 ccccttcatc ctctgtatag ttcttgatag tggatacagc agtagttcct ttggtcaatg
     9001 taccagtttt atcaaagaca aaagtatcga ttttggaaaa agtatccatt gcttcaccgc
     9061 ctttgactaa gatgccccgt ttcgctccat taccgattcc tgctacattc gaaacaggcg
     9121 caccaatgac taaagcgcca ggacaaccaa gaaccaaaac agtgattgct aaacgaaaat
     9181 cacgactaat cagaccaaca accaacgcaa taagtaaaac ggttggagta taatactgag
     9241 aaaatcgatc aatgaatttt tccgcatggg atttggtatc ttgtgcttct tctactaatt
     9301 cgataatttt ggcaaaggtc gtatcatctc cgaccttagt tgcttcaatc gtcacgtagc
     9361 catctgctaa aatcgttcca gagaagactt catctccact gattttgacg acttgtcgtg
     9421 attcaccagt cactgcggct tcatccgcat accctttccc gtcaatgact cgtccgtcca
     9481 caggaatagc tccaccagtt tttactaaga cgatatcgcc ttcttccacg tcatcgattt
     9541 ccacttcttc agtcgttcca tcttcttgga caacactagc agtagatggg gccatctcgg
     9601 tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt ttgttccaga tagctaccaa
     9661 acaaaaacaa gaatgtaacg atagctgact ccttgtattc tccgatcata aaggcaccaa
     9721 gcacagcaat agtaacgagt aaatcaattg agataacttt gactttcact gcctgatacg
     9781 catggatcat aataggtatg accgcaataa tagaagctat ggctagagca atgttataga
     9841 acaagacaaa accagtaaat tttccgataa aaccaaaaat aatcaacaca gctgataagg
     9901 cggtgatatg atttcgatgc tcttggatcc acttttgtaa tttcatcttt ctccctcttt
     9961 tcctttcatt cttacgagac taagtataga tacttatcca tcataaaaaa ttgaccacta
    10021 tcaagtttag tcaaaaaggc ggttttcttt tggtagaata cactacaaaa aatagttagc
    10081 aagaagactc actaaaagtg atcttgccaa ctattgtcgt ttctatcgaa attttcattg
    10141 aatgattttc ctgtacagca tacatcggag atctatttct ttatctaaga tttttatctt
    10201 gctaattttt tcctttagtc tttcaaatcc acgtcgttca tagaattgat acgtacagcc
    10261 actatctgta aaaaggaata tttcttttcc tttctctctt ttttcaaatt ctgttagcaa
    10321 cttggtaccg atgccttttg ttttcaaatc aggatttgca gctaaaaaaa tgatctctcc
    10381 atctggcgaa tgcttcttta aatatgctgc aagcatttgt tcatttgctg tctcgtaagc
    10441 tcctttactt tctttagcaa atatattttg taaaaaatcg aatatcttaa catatatttg
    10501 ctgtccaaaa gaacgatgtt tctttttttc tccttttatc tccgccagca aaacacctgc
    10561 caactcgtcc tctgcataca aagcaagaat ttgagttgct cgattcaact ccatatacaa
    10621 aaaatatctt ccgtaaagat tcagcagaag ttcattttcc atatatctct caaagttcat
    10681 tccttcaaca gcaaaataga tagctttttt gtaatctttc ttttctaagg gtttaaaagt
    10741 tagtttcatc atttcgcctc ctcaacttca atgattgtgt tatacttaca ttataataga
    10801 cacagtgtct actatctata atcaatcatc tataagtttg tcgattaagc gacagctttg
    10861 ctttgatatg tcggataagg agataggtaa atgaaaaaga cagatagacg cgtcaaaaaa
    10921 acagaaaaag ctttggctga aacactatcc acattattag taaataaaaa gattcaagca
    10981 atcactatac gcgaactaac agaaacggca gatgtacatc gttcgacttt ttatacacat
    11041 tataaggata tctatgatct atatgatcaa ttagaaagca acttttttcg ggatctaaat
    11101 gaaattctct cttatgatcc agcccatagc tatgaggaac tatacactcg cttgattgac
    11161 tatgtttacg caaatacttt tttatatcgc ttatttatcc tcaaccatgg tgatatttcg
    11221 tttcagaaga aagttaaaca agttattgaa gaaaactatc tgaacatatg gctatatgaa
    11281 gacaagaaaa cgattatcac agaagaaatg agattcttta ctacgtacca cgtccaaggt
    11341 tgtctatccg ttctcaatcg gtgggtagaa agcaattaca agtactccaa aaaagaatta
    11401 ttagaactct tacagaaact gaatgatcac atagagaaaa tcatgccttg aataaaaaca
    11461 tactaagctt agttaagaag aaccgtaacc ttctcgtata acaactgaat cttatttagc
    11521 caaattgttt actgaagatt ttttgagtaa aatttctata attcctactt attgaaaagt
    11581 agtttgtagt gctgcttctc cctctctaag tgatatatct tgttgtacca atcaaattca
    11641 cctcatcatt aataaattac ttttaggcta atttattaca acttataggt tgaagctgta
    11701 accaatttat agtcatatta tatttttatc atccagttta ttgaattctt ctcgtttacc
    11761 tcaattcatt ttcaagggga atca
//
LOCUS       DAZUKA010000088        11631 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000088 DAZUKA010000000
VERSION     DAZUKA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.091"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8PN_001919"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8PN_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4862057.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8PN_001920"
     CDS             complement(323..892)
                     /locus_tag="ACG8PN_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4862058.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8PN_001921"
     CDS             1078..1980
                     /locus_tag="ACG8PN_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4862059.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8PN_001922"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8PN_001922"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4862060.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8PN_001923"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8PN_001923"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4862061.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8PN_001924"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8PN_001924"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4862062.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG8PN_001925"
     CDS             8548..8733
                     /locus_tag="ACG8PN_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4862063.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG8PN_001926"
     CDS             complement(8795..9991)
                     /locus_tag="ACG8PN_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4862064.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG8PN_001927"
     CDS             complement(10161..11597)
                     /locus_tag="ACG8PN_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4862065.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3424 a         2483 c         2044 g         3680 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg g
//
LOCUS       DAZUKA010000089        11623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000089 DAZUKA010000000
VERSION     DAZUKA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.092"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..516)
                     /locus_tag="ACG8PN_001928"
     CDS             complement(139..516)
                     /locus_tag="ACG8PN_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862066.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(816..1697)
                     /gene="galU"
                     /locus_tag="ACG8PN_001929"
     CDS             complement(816..1697)
                     /gene="galU"
                     /locus_tag="ACG8PN_001929"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4862067.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1740..2759)
                     /locus_tag="ACG8PN_001930"
     CDS             complement(1740..2759)
                     /locus_tag="ACG8PN_001930"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4862068.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3392..4396)
                     /locus_tag="ACG8PN_001931"
     CDS             complement(3392..4396)
                     /locus_tag="ACG8PN_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4862069.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4409..5458)
                     /locus_tag="ACG8PN_001932"
     CDS             complement(4409..5458)
                     /locus_tag="ACG8PN_001932"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4862070.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5475..6296)
                     /locus_tag="ACG8PN_001933"
     CDS             complement(5475..6296)
                     /locus_tag="ACG8PN_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4862071.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6283..7062)
                     /locus_tag="ACG8PN_001934"
     CDS             complement(6283..7062)
                     /locus_tag="ACG8PN_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4862072.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7079..7549)
                     /locus_tag="ACG8PN_001935"
     CDS             complement(7079..7549)
                     /locus_tag="ACG8PN_001935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4862073.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7561..7980)
                     /locus_tag="ACG8PN_001936"
     CDS             complement(7561..7980)
                     /locus_tag="ACG8PN_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4862074.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8061..10838)
                     /locus_tag="ACG8PN_001937"
     CDS             complement(8061..10838)
                     /locus_tag="ACG8PN_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4862075.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11029..11253
                     /locus_tag="ACG8PN_001938"
     CDS             11029..11253
                     /locus_tag="ACG8PN_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862076.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3348 a         2457 c         1894 g         3924 t
ORIGIN      
        1 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
       61 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
      121 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
      181 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
      241 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
      301 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
      361 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
      421 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
      481 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
      541 attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt
      601 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
      661 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
      721 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
      781 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
      841 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
      901 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
      961 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     1021 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     1081 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     1141 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     1201 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     1261 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     1321 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     1381 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     1441 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     1501 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     1561 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     1621 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     1681 gcttttttga ccttcacttg ctgttcctcc taaacgacga ttaactattg atcatctatt
     1741 taacgaagag aaaattcatt ctccattttt ccgtcacgta acatgatttc ttttgctgct
     1801 tttttgatat cttctccatt atacaaaaca ttatagattg tttgagtaat tggcatctca
     1861 acacctagct gttgagctag ctctactgct gcttttgtcg ttgccacacc ctcaacgacc
     1921 attcccatgt tttctagaac ttcttccagc ttatgtccct gtcctaacaa gttaccggca
     1981 cgccaattgc gagaatgaac acttgtacaa gtaacgatca agtcccccac accactcaaa
     2041 ccgataaatg tcagaggatt ggcacccatc gcgacaccaa gacggctgat ttctgccaat
     2101 cctcgagtca tgattgctgc ttttgcatca tctccgaatc ctaatccatg gatagcgccg
     2161 gcaccaattg caatgatatt tttcagtgct gcaccagttt ctacgccaat cacatcatta
     2221 tttgtataaa tcctgaaata atcattcatg aacagttttt gtacataggc tgctgctttt
     2281 tgatcaatac ttgcagctgt aattgttgtg atatcatgta ctgccacttc ctcagcatgg
     2341 ctaggaccag ataatacaac aattgcctgt cttttttctt ctggaatttc ttcagccaaa
     2401 acttctgaga ttcgtttatg cgtatcttgt tctagtcctt tacttgcatg tataatgatt
     2461 ggcttagtat tcattttacc aataagttcc tgtgcgactg aacgaatcgc ttttgttgga
     2521 acaacaaata aaatagcatc tgcgttatcc actgcttctg ccaattcttt atatccgata
     2581 atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat ttgtatgata cttattgatt
     2641 tcatcaatct gttcaggtac atttccccaa atacggacat catgcccgtt ttctgccaat
     2701 gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa ctgcgatttt ttgtttcatg
     2761 gatattcctc caaataaata attgataatt tccattatag tatacatttc ttaaagttgg
     2821 gactaatttc cattgttttt aagtgtttgt ttacttcttt tatcgttttt acttgaaata
     2881 atcaatgata tgttacaata gtctacattt tatttttaca ctcgttatgc tttgcttgtt
     2941 cctaagagga agcagttttg acaatgttac taccagcatt actattagga ggttaggaaa
     3001 cttctcctag tgagtattga tttaaaatac ataagagaca gattgaacat ttctaaatct
     3061 gtcaggtcct gcaacggaca gattgtctct gttgacggtt gacataggaa cttttgtttt
     3121 agtcaacatt ctggatacaa aacgctatgt aaactggcgc aactcgttgt acttggactt
     3181 ttactacgga tccatgcaac aactaaatag tactattgca taacaaaaaa tggttggcac
     3241 ccacatgtcc cattaaacag attataagtt gaactgtaga taatgcaaaa aatgtgtttt
     3301 ataacgtaat tgatgccaat attttaaaaa aataaaggga gcagttatcg ctgctctctt
     3361 tattttttat attcttttta tccagtctat cctaataatc aaactgacga tagtagcggc
     3421 gtatctctaa tggatggcag ttcaactgct ccacatgagc atcgatgcgt tgtgtcacta
     3481 catgcatcaa taagaaagac aaaacacgtc cgcgatattt ttcactgata ccttctactg
     3541 gatagtcttt tgtgtctatc aatgtataat tgctgcaaat ttttggcaaa agtttagcca
     3601 cacgttcaga taacactctc tgttcatctt cgcctaaaaa taatgtgact ggggttgttt
     3661 cttctacgat ctctaacgtc ccgtgaagaa actcggcagc atggatagat ttcgatggaa
     3721 gccagctttg ttcttcccag taacacatgg catatgaata aacagctccc cattgattcc
     3781 ctgctccaat aaaataatgc atactatcac gtcgatgttt ttctgcaaat gataagccaa
     3841 atgcatctgc ttttttctct gcttccacta accctgttgg taaataacgt tcgagctgtt
     3901 catagtattc tgaataagct tcgaattctc cattcaaata catcaatcga tctgctgcca
     3961 taaaaaattt gatttgttct gttccaggag ctggataagt gaccacaaaa tcgcatagct
     4021 gagacaactt actgttcgct ttatcgataa atccaatcag tgtcgctcct actttcttga
     4081 tttgttctac tgctttcact acttcttgag ttgtaccggt aacagaggac aaaataacga
     4141 tagaatcttt tgttaatctt ttgtttccag tagtgtaata ttctgcagca tgctgtacaa
     4201 agacaggcaa gttgcttttc ccattcatat aagtgacagc ttgcatcgag gaagcatacg
     4261 ttccaccgat tcctagataa tagacagcgt caaatttttc cgcataaagt ttatcgatga
     4321 tttcttctac ttgcggacgt aaagctagtg cgccttttat tgctttgatt tgttcttctt
     4381 cattgaattt cagcatgatt tccacctctt ataatttact tttcaagact gtatcaaagt
     4441 cttcaaaaat ctttttgttc aagtcaatac ctttagctac tgcggtttta taagcgaata
     4501 gttgaaacgg aacgacaagc gctaatacag acagatattc atcacattct attgctaatc
     4561 caagtgtttt ctcgtttctt gctttttttg tggtaatggt gagagtctct ccaacgtacc
     4621 gactcatata tctttgcaac ttttgcgaac gttccttatt tacactttcc ccttcaataa
     4681 agaatagcaa atgcgaagca tctgcctcca ggtatgggcc gtgcatataa gcttctaact
     4741 caaatccttg tgacggaacg cggattgttt cagtcaattt cgtctctgct tctttagctg
     4801 ttccccagtt tggaccatat ccaatggtga cgaaacgctg tgctaaacga aataaacttt
     4861 gatatccatc aaaaaattct tccgtttttt gaataatcgc tggtaaatgg ttgattattt
     4921 tgcgcaactg ttgcatgtag tcttgttcta tttttttgct gattttattt ttacttattc
     4981 caattcctat ccctaagagt agtaactgta aaacagtcgc agaatagcct tttgtaacga
     5041 agcctactga ttctaccccg gcacctaaat caataatttg tgcagcttct aaagcaagtg
     5101 gactttgcac gtcatttgtg atagcaacaa caggtaaccc ttgtttttgt atcattttta
     5161 ctgcttcaac agtagaagca cttttcccgc tttgtgacac agcaatcaca gtatcgattg
     5221 aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc ttgaatatcg acagtaagat
     5281 caccataaga ttccaatgct ggcttcgctg ctaaacaagc gttataagat gaacccgttg
     5341 ctaagatcag taaattcact gtttttttca tatgttctct tgtcgaaaaa tcattctttt
     5401 tcaagatcgt acttagtact tccttttctt catagatata atcttgcatc gtaaacattt
     5461 tctctgctcc ttttttaaaa aatccctatc cacgaaccaa aaattccaaa gaaagctagt
     5521 ccgatcaaga tcgttaatgg tttcacttct ttcttcaata gataaaacac tatgccaaat
     5581 gctccaagac ataagatatt tggaacgata tcatcaaaaa tactttgaac agtcacagca
     5641 ttttctcctg aaccgatttt taaaggaata ttgatggtga tcatcgtggc agtcattgct
     5701 ccaacgacca tcagtccaat aatagacgcg cctaatgtca aactttccat cataccgttt
     5761 gcctggatct ttttcaaaaa acccgttcct aagcgatatc cccaacctgt tgctagatag
     5821 cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca agatagggcc taaaatattt
     5881 ccttgcatgg ctaaagaggt acccacacct gtggcaatca atcgcaaagt tccccaaaag
     5941 aaagaatctc ctaaccctgc aagaggtccc atcaaagatg ttttgatact atcgatcgca
     6001 gtcacatcaa attcaggatc atccttattt tcttcctcca ttgcagccgt gattcctaaa
     6061 atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta gatgccgttt caaagctgct
     6121 gccatttctt ctttcgactg atataacttt ttcaaaatcg gaatcattgc ataaacataa
     6181 gccatattca tctgacgttc ataattccaa gaaaactcca tttgaaaaga acgccaaaat
     6241 acgtgattta gctctttttt cgttattttg ctgtttttga tctcagaatt cttcatcttc
     6301 ttctacctcc cctgcagttg tttgaagtgg tagttgacgg acattcataa tattgactac
     6361 cacaactgct aaaatcgcac caaaaatagc tactccggtc acaggaatct ctaagtaaga
     6421 agctatagca aaacctaaga aaaaatatgg tgcaacttgt ttgttgatca gcaaacgagc
     6481 aagcatcgca aaaccaagag ctggaatcaa ccctgtagct actgacaacc ccttttgaac
     6541 gaagtcagga atcatattta atagatttcc gatcgtattg cttcccacca tataagaaac
     6601 cattaccaca acaccaagca tcaaggaaag accgataccg gcaagcaagt gcatatgctc
     6661 gactccttta taatttcctt cttctgcata attgtctgct ttatggttca taattggaat
     6721 caaaagccct aagtaaatat ttttcaatat caatgtcaac gtagcaatcg gcaaacctag
     6781 taaaagcgcg gtttctgtac cggctccagc tgtaatagca aaagccgtcc ctaaaatccc
     6841 accggtcaca acatcaggtg gaatcgaagc acctactgag aacgatccga taaatgctaa
     6901 ctctaaagtt gcgcccataa tgatcccagt ttttacatca cctaacacga ttcctgtaaa
     6961 cagccccgtc acaatcggac gagaaagcaa cgatgtccct agtgcatatt ctgattgtgc
     7021 gacaaatgca acaattccta ataagactgc ttgtacaatc attgaggttc ttcctttctt
     7081 acaaaacttc tcttacatcc acttttttat ctgctggtac ttgacggatt tctacttcta
     7141 tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc tggtaaaata tttaccgctt
     7201 ttccgatgct gcgagtacca ggttttgcct tgatccctcc taagttgatg gttttgattt
     7261 ctggatacgc ttctgccagc ttatatgcat cctcgatcga ttccactacg atgaataact
     7321 tatatttatc tgtcaccccg cttttcaacg cggcaatcga gtcatcaatt gatttgatca
     7381 ctagtttgac tccttgtggc ttggctaatt tgatcgttgt ctttcgtatc tcattcgtag
     7441 gtacatcatc attagcaatc aaaatacagt ctgcgcctat tgtttgtgtc catgaaaaag
     7501 ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa tacaatcatt tttcttcctc
     7561 ctaaaattcg tcttcttcta ttttctgatt gagccgctcg ttacaaaact ggatcatttt
     7621 ttttgaatca gttagcacct gctggatcaa aacagttgtc tctacttcag cgtccaaccg
     7681 agatactagc tcaatcaata gtggcaaatt caatccggca acgagataga catgatcccg
     7741 attgatatat tgtaagaatt cgttgttgac acttccgcca aaaagatccg tcagaacaat
     7801 gatttcttta tctttgtacc tcataaaaag ctgccttact gtttccgcta aatcaaaatt
     7861 ctcatcagta tagcaatcga atacagtaat agaatgattt ttcccataga tcagctctaa
     7921 tgaatctaat atccctgaag caaattttcc atgggaagct aagataattt ctctatccac
     7981 tttttcatct cctcttttca cttacacata ctaaaaaagc aagttgcatg ccaaaattag
     8041 tggcaacaac ttgcttatgt tcaaaattct tcttctatcg agagaaactc tgtatcttca
     8101 ctcaaaatat catagatata ggcaatctca gaattgggga tcatgacact ataatgctct
     8161 tcaatgacac taaacgcaga tttaatagca gataaccctt ctttttgaca ctgcttccat
     8221 ttctctaatc cttgataggt ttcgattggc atttgtcgga tcaatcgttc aatgagacag
     8281 ctcacatgga catacaacgc taattttttg ggattgctga tgcttcgatt caactgcacc
     8341 tctaattctc tcataaaatg atcgatttct cccatcacct tctctgtatc tagaatagtc
     8401 acggaatcca gtactttttc caatgaaaaa ttacgaatga tgcgctgatt gaatatttcc
     8461 ttctctgaag cattcatagt agtgtccaac cattcagcta gtacagacat cttttccccc
     8521 gagatgaggt cttctaatga aagatagtcg atattggtga tatcaggatc tgtggtgcca
     8581 atcattccta cgacttcgta tatggaaaat aatgggtcac tgtttttctt atcttttaat
     8641 tgctgatatt catatgggat gattttcaac tgacaagccg taggtaagct tttttctagc
     8701 agttcactta agtggaccgc cgtaccgatt ccagtgaagc aagtagtaat gagtgctttg
     8761 ggtttgtttt cctctggata gagcagtcgc cattctggct ggatctgtcg aacagtctct
     8821 tctgctatct cttctaatga tacatttttt tgcaggcatt cccctactgt gattgctaaa
     8881 ggtgtcgtca cattattcaa aatcaaaagc ggtacggata actgtttttt aaaaaactga
     8941 tggatttctt tcagagatcc catgtctact aatatgatca atccatttga aacatcgctg
     9001 ttttcgcaat aatcaaggat ttctgaagca atctgctgtg gtgtgacatc tagtggcata
     9061 tcaaatgatt caaatagatc ctcttgtaat aaacgattta ctacatttgc catactgcta
     9121 gcagttgcat aaccgtgagc agcaatcatc gcttttggca gtcgtttttc ttttgaccat
     9181 ttcgcttttt ttagatagat cgtcagtaaa atccgatcca tacttgtgtc ttcaatatct
     9241 aatttgatct tacttaattc cagaattcgt tggacatagt gataactaga tggataagac
     9301 tgagaaactt gttgttctaa ttctttgatt agttggataa tatcatgttc ttccggtaac
     9361 catttgatag tgcttcgctg atagagatag taactcaagg catagacact gtttccatcg
     9421 aatttgattt gataggcagt ttccatttgt cgaaaggtct ctcggataga ttgtgtccaa
     9481 taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa acagcaaaaa atcaaataat
     9541 tgaatcactt cttgctttaa ttttccttcg caagaatata gcagacagtt atttttcttg
     9601 aaatcaatta ggatttgctc aaaactttta acgattcgct gctgttcggg ctgttgcttc
     9661 tccattaatt gctctaaggt ctggtcttgc gtaatataga ttccttgctg tgctgtattt
     9721 ttagtaatag gctgcgcaag tagttctttt tgcaaatgat ggatcgtgac atggatttca
     9781 ggctgttcac gctgttcagc atatgctttt gctgctgaaa ctttgacgat atttttcagc
     9841 tctcctatgt ttccggataa tttctgactt gttaaaagtg acaaagctgg tccactgact
     9901 gacaaagggc tccccatttt tctttgttct tcgagaaaaa aagagtaaat cagttctagc
     9961 cgttcattac gactacgctg attaagtgca ggtagttcga ttcggatagg tattcttcga
    10021 atgaaagttg ttaagaaatg actagtaatc tcttcagtcg tagcaaaaaa gagacgtgtt
    10081 ttgatccggc gtgacaaatt aggttctccc atacgataaa tcacaccttg atcaagatag
    10141 gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta cctcgtctaa aaacaagatc
    10201 ccaccatctg ctgcttcaaa agcccctttt ttattttctt ctgcaccagt gtacgctccc
    10261 ttaacatgtc caaacaaatt gctagtcaaa agttcaggat tatctgcata ttgtgcacaa
    10321 ttaaaagtga tgaatggtgc agaatcttct agcagatcat gaagcagaca atactgatag
    10381 accaaattaa ctaaataact tttacctgtg ccactttcgc ctgtaatcaa aagtggtaat
    10441 ccgccatctg gatagtaaag agctgttttg atttgttcga tactttcttt caaactggcg
    10501 tcgtatccga tcataaaaga gaataagtct tttggttgcg cgaaaaaagg ctgttcctct
    10561 ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact taaaaaattg tttttcaaaa
    10621 gcttctttgt ggaaataaat cactggtcga gaattgattt tgactagtag ttgctcatca
    10681 tttaactggt tcaaataatg actgactgtg tttcttttta ccccaaaatg atcagctaac
    10741 ctttgtgctg taaagacctc gttcatatgt tctaaatcga aaaaggctgt ctggttctct
    10801 aagtaacata aaatttcctt cttcaacaac ttattcaccc ctcctccatc atagaaaacg
    10861 gattcatttt gtcaataaaa tctttaataa agaattgaaa aatatatctt attatagaca
    10921 gcagcctact tatcccttat aatttttatt ctaaaaaacg tacagttcct tccagttttt
    10981 atactggaat ttcaacatat gaaagaataa ggaggctatg actattcaat gaaatcggat
    11041 aaatataaaa acaggaaaca tacttcctgg aaaacttttc gagaaatcat cggatcttat
    11101 ctcgctgtag ttgtattttt gcctttgctt tatatggcta tgacagaaca aactcgtttt
    11161 attggcgtag atacgcaata tatagccgaa acaggatttt tattcatctt tatcttgatt
    11221 tttgcgttct ataaatcctt ccatacaaaa taagattcac agatttcaac tttcctgtct
    11281 gtttttagct attacaaatc cttatatgtg gtataacaac aatttattgg cataatgagg
    11341 aaaaacgagt tatgatgctt tcagagagta aagaggtact gttgtcacca tcctttttat
    11401 tgattacgta caccactctt agttttttcc cattgattac gaaaacaagg tagaaacatt
    11461 gatgttccta ccttgttttc tttttttatt ttcagatcaa aatctcaagt ggcttttgac
    11521 ttgatgtcgg gaggttgtct tcgttccatc aaacctgata gaatctgcgc caaaccatag
    11581 ttttctccac tattatttat tcctctaaga tagtacccac aat
//
LOCUS       DAZUKA010000090        11594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000090 DAZUKA010000000
VERSION     DAZUKA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.093"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8PN_001939"
     CDS             6..305
                     /locus_tag="ACG8PN_001939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4862077.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8PN_001940"
     CDS             378..1739
                     /locus_tag="ACG8PN_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4862078.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8PN_001941"
     CDS             1761..2021
                     /locus_tag="ACG8PN_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862079.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8PN_001942"
     CDS             2035..2787
                     /locus_tag="ACG8PN_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4862080.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8PN_001943"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8PN_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4862081.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8PN_001944"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8PN_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4862082.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8PN_001945"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8PN_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4862083.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8PN_001946"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8PN_001946"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4862084.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8PN_001947"
     CDS             7161..8558
                     /locus_tag="ACG8PN_001947"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4862085.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8PN_001948"
     CDS             8639..9079
                     /locus_tag="ACG8PN_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4862086.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8PN_001949"
     CDS             9227..9553
                     /locus_tag="ACG8PN_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4862087.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8PN_001950"
     CDS             9554..10303
                     /locus_tag="ACG8PN_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4862088.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8PN_001951"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8PN_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4862089.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8PN_001952"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8PN_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862090.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3657 a         2013 c         2355 g         3569 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttat
//
LOCUS       DAZUKA010000091        11315 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000091 DAZUKA010000000
VERSION     DAZUKA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.094"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            277..1536
                     /gene="lysA"
                     /locus_tag="ACG8PN_001953"
     CDS             277..1536
                     /gene="lysA"
                     /locus_tag="ACG8PN_001953"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4862091.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1523..2323
                     /gene="dapF"
                     /locus_tag="ACG8PN_001954"
     CDS             1523..2323
                     /gene="dapF"
                     /locus_tag="ACG8PN_001954"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4862092.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2326..3207
                     /gene="dapA"
                     /locus_tag="ACG8PN_001955"
     CDS             2326..3207
                     /gene="dapA"
                     /locus_tag="ACG8PN_001955"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4862093.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3204..3920
                     /gene="dapB"
                     /locus_tag="ACG8PN_001956"
     CDS             3204..3920
                     /gene="dapB"
                     /locus_tag="ACG8PN_001956"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4862094.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3926..4621
                     /gene="dapD"
                     /locus_tag="ACG8PN_001957"
     CDS             3926..4621
                     /gene="dapD"
                     /locus_tag="ACG8PN_001957"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4862095.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4637..5638
                     /locus_tag="ACG8PN_001958"
     CDS             4637..5638
                     /locus_tag="ACG8PN_001958"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4862096.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5635..6996
                     /locus_tag="ACG8PN_001959"
     CDS             5635..6996
                     /locus_tag="ACG8PN_001959"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4862097.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6956..8107
                     /locus_tag="ACG8PN_001960"
     CDS             6956..8107
                     /locus_tag="ACG8PN_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4862098.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8082..9251
                     /locus_tag="ACG8PN_001961"
     CDS             8082..9251
                     /locus_tag="ACG8PN_001961"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4862099.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9426..10805
                     /locus_tag="ACG8PN_001962"
     CDS             9426..10805
                     /locus_tag="ACG8PN_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4862100.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11086..11315
                     /locus_tag="ACG8PN_001963"
                     /pseudo
     CDS             11086..11315
                     /locus_tag="ACG8PN_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3771 a         1965 c         2514 g         3065 t
ORIGIN      
        1 aaattgaaaa tatccgaact attccgaact atattgaagc gaaaatattt tttcatcact
       61 aaatagtgtt gaatttatgt ttttatgtat aaatagcttc taatagttcg ggtattttgt
      121 ttttataacc atttttattc ttatttttaa aaaaatattt cgaaatatac tagacaattc
      181 agaaaatttt gtttaatatt attccaataa aattcaaatc tttatcaaat gaaattccaa
      241 taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga cagaattgat aattggcgga
      301 gtcacagcaa gcacattggc agaaaagtat aagacacctc tgtatgtgta tgatgaagaa
      361 aagatgagac aaacgatgct gtcattcaag cgaggctttg tatcgaaacg ttttgataca
      421 aaagtgcttt acgcctctaa agcattccaa acgattgaga tgctgcattt agttcgtgaa
      481 tatggattcg ggctagatgt tgtcagtggt ggggaaatct ataccgcttt gcaagctgat
      541 tttcctagag atactatcta cttccatggt aacaacaaga cgccagagga actgatctat
      601 gcacttgaaa atgatttgtt gcatattgtt gccgataatt tgatggaagt tgaattatta
      661 gcagaattaa gccaacgcta tttaaaaaaa atctgtatca tgcttcggtt gaatgtcgga
      721 gtagaagcac atactcatga atatatcgtc actgcgcata ttgattcaaa attcggcatg
      781 agctatgaaa gtaaagagtg ccaacaagcc cttcagttga taaaagaaag cagctatcta
      841 gagttagaag gatttcatgc tcatatcggg tcacaaattt ttgatgttac tgcttggtta
      901 gctgaaatcg acaaattgac gaattactta accgattttg accaacctct ttccttgaat
      961 cttggcggag ggtttgggat tcgttataca caaaatgatc agccgatccc agtcgaagag
     1021 gcactaaaaa aactgattga atatacggaa gaagctttgg aaaaacgaaa actgaccatt
     1081 cgtcaattac tgatcgaacc gggtagaagt attgttggag aagctggtac aacactatac
     1141 acgattggtt tcatcaaaaa gacaccacat aaaaaatatt actttgtaga tggcgggatg
     1201 acagataata tccgtccagc tctgtatcag gcagaatacg attgtgattt agcagcaaaa
     1261 ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa aaatgtgtga atcaggcgat
     1321 gtggtaatta aagaagctta tttgccgaaa gcagaatcgg gtgatctttt agctgtttat
     1381 tccacgggag cttatggata ctcgatgagc agcaactaca atcgagcgac acgaccagca
     1441 gttgtttttg tcaataatgg ccaatcaaga ctgattgttc gcagacaagc attttctgat
     1501 ctactaaggg gggaagtttc ctatgatcct tcataaatat aatggctgcg gaaatgattt
     1561 tatcttatta gattacgcag aagacacaga ttatagtcaa ttagccgctt ctttatgcac
     1621 aaaagaaaaa tatgatatag acggattgat cgccgtaaaa atcgatccgt tggaaatgat
     1681 ctattacaac aaagatggca gccgcgcgcc gatgtgcgga aatggcattc gctgttttgc
     1741 tcgatacgta aatgatcaga acatgatgaa tgaacaggaa ttcaacgtag agacattagc
     1801 aggcacaaga aaaattacgg tgttggatcc ctcacctttt tattgcagcg tagatatggg
     1861 agtgccagat gattctccgg aaaaaatcaa agctaatcaa acttatccaa taaaaaacca
     1921 ggaaatagag atcaatggtg aaaaagtaac gatcactagt ttatttttag gaacgattca
     1981 taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg agaaaaagag gtgaactgat
     2041 ctgtcatcat cctttgttca aagaaaaaac caatgtcaac tttgtagaag tagtcaatga
     2101 acaagaattg atcgtccgta cctatgaaag aggagtaggt tggacacttg cttgcggtac
     2161 aggttgctgt gcctcttatg tggttgctaa ggaaactggg aaaatttcag cagatcaggt
     2221 agttgttcac ttggaacaag gagacttgat gatctctggt gaggatgcga tacaaatggc
     2281 tggcccagca gttcatgaat ggacaagaat attggaggaa tgaagatgat aaaaggttcg
     2341 atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag tagattatgc tggtttggaa
     2401 aaattgatcc aattccattt agatgaacaa acagatggat tattagtttt aggtacaaca
     2461 ggagaatctt ctacgctcac acaatctgag gaggagcaga tccttcaact gacagtaaag
     2521 aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa cgaataatac gaaagaaacg
     2581 attgaaaaag cgaagcattt tgcttctttg ggggcagacg cacttttggt catcacccct
     2641 tactacaaca aaacaagtga tgcaggacta gctgctcatt ttacagcaat tgcagaagcc
     2701 tcaccgattc cgcttatttt gtataatgtt ccttctagaa caggcatgtc gattccaatc
     2761 catgtattag taaatttagc agaacatcct aatatcattg gtttgaaaga agcctcagga
     2821 gatatggctt atgtgatgga tgccgctcgg ttgattggag aagaattctt cctttacagt
     2881 ggaaatgatg atttgatcct accagtaatg agtgtaggag gaagcggtgt aataagtgta
     2941 tgggcgaata tccaacctaa aatagtccat gaattagtaa aagatacgca ggacggaaga
     3001 tggcagcaag caaaagaaaa gcaattaaat gcacttgaac tgatccatgc cttgttcagt
     3061 gagaccaatc ctattcctgt caaagcagcc atgtcattat tagacttacc ttccggacct
     3121 ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga aacaattagc tcagcttttg
     3181 ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat tggcagtgga cagatgggaa
     3241 atagagtcga agaagttgca caagaaagag gagatacggt ccttctttta cggacggcac
     3301 ctaaagaagc aactacgctt gcttttacgg aactgccaga actgcttatt gatttttctc
     3361 atccagataa tttagagaaa atcctttcgt atagcttatc atatcaactt ccgttagtca
     3421 ttggcacgac aggttataca caatcacagt tccaagaaat aaaagaacat gccaaacatg
     3481 tacctgtgat gtatagtgct aatttttcgt tcggcatcat ggtgatgaat caactaatca
     3541 agcaagcagc tgcaatgttg caaggctggc agatcgaact gattgaaaaa catcatagtc
     3601 gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct tcaaacaatc caagaagtgc
     3661 aaaagctgca gcccgtatac aactggcaag ggaaaaaaag agaagaaaac caaattggtg
     3721 tacacagtat tcgtgcaggt tctttacctg gtgaacatga tgtattgttt gctgctactg
     3781 atgaaatatt tacgatcaaa catgaatctt tttctaatcg aatctttgct gcaggcgcag
     3841 tagaagcagc ggactggttg aaagatcagt caccaggtta ttacaaatta gaagatttat
     3901 taatggacaa aggggtgtaa aaaacgtgga agctcaagaa atcatccgct acattcaaac
     3961 ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta accgagccaa tcgaatttaa
     4021 agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt tttggtgact atgagacgat
     4081 tgctccggtt ttagaagctg aatcagaaaa aatcattgaa atcgaaatcg aaactgctgc
     4141 aagatacagc gcagttcctt tgctagatat taaaaaaatt aatgctcgta tcgaaccagg
     4201 tgctattatc agagatcaag tgtcaatcgg caacaatgcc gtgatcatga tgggagcgat
     4261 tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt gatatgggtg ctgtcttagg
     4321 tggacgagcg acagtgggca aaaactgcca tattggtgca ggagctgtct tagcaggtgt
     4381 gatcgaacca gcatcggcca aaccggttat cgtagaagac ggcgtactgg taggtgcgaa
     4441 tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca gtcgtggcag caggagcagt
     4501 ggttttagaa gatgtggcag cagaaactgt tgtcggtgga atacctgcac gtgtattaaa
     4561 aataagtgat gatcagacaa aagaaaatac agcattgatt gctgcgttac gcgaattata
     4621 gaggagatgg agaaaaatgc gcgaaataga tacctgtatt gtgggggcaa ccggattagt
     4681 cggccaaacg atgctcaaag tgttagaaga atatgatttt ccagtaggac gattgaaatt
     4741 attagcttca aaacgttcag ccggtaaagt ttgtgtattc aagaaaaaga aacacactgt
     4801 acaggaattg acagaaactt catttgaagg atatgattta gcgctatttt ctgcaggtgc
     4861 ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag ggactatttg tcatagataa
     4921 ttcctcagca tggcgggaag attcggatat tccattaatc gttccagagg tcaatctgga
     4981 agactataca ttgaatcggc tgattgcaaa tccaaattgt tcaaccattc aagcggtact
     5041 tccgcttaaa gctttacaag aagcctttgg tatccagcga gtcaattatt cgacctatca
     5101 agcagtttct ggttccgggc aaaaaggcat tgaagatctt catcgtacgc aaaatgacga
     5161 ggaaccagtt ttgtatcctt ataatattag ccaaacggtg attccggaaa tcgatcagcc
     5221 tttggaaaat gggtatacaa aagaagaaca gaaaatgata aatgagaccc gtaagattct
     5281 gcatcagcct gctttgccag tatcagcaac ctgcgtacgt gtgccaatcg aaaatggaca
     5341 tggagtctct gtggcagttc aactgcaaaa agagtttacg acggaagaaa ttagagagtg
     5401 tctcagccag tttccaggga ttcaattaga agatgatcta gcagcacata aatatccgac
     5461 gagcattctt gctagaggaa cagatatggt ttatgttggg aggatcagaa aagacacttc
     5521 tttagagaac ggattgttgt tctatacgac tgccgataac atacgcaaag gagccgctgc
     5581 gaatgccgta cagattgctg cagctttaat gcagaaaata gcggaggtgg caagatgagt
     5641 aatacgctga aagtagcgaa attcggcgga acatcgatgt ctcatgaaga gcaattcaaa
     5701 aaagtccgta caattgttct ttcagatccc aatcgccaag tgatcgttgt ttctgcttta
     5761 ggcaaacgta cgtcagatga tgacaaagta acagacttac tttatttgat ttatgcttac
     5821 cttcagcatc atgttgattg gacacccctt tggaaaagga tttgtgatcg tttcattcaa
     5881 gtaagagatg ttcttgccct gcagttacct attgaagatg aactagcacg catacagaaa
     5941 caactggaag acgaaaagat aactgaagat tatttagtaa gtagaggtga atatctgact
     6001 gctcaactga tggctgagta tcttggttac cagtttattg acgcaaaaga cctgattttc
     6061 tttgactatg atgggaaaat cgatcaggaa aaaacagcac aggaaatcac aaaagcattt
     6121 aaaagatatc cgaaaatggt tgttccaggg ttttacgggg cgaaccctgc aggaaaagaa
     6181 aaattattgg gccgtggggg aagcgacatt accggcgcga tactggcgaa gatgttgcaa
     6241 gcggaaagtt atgagaattg gacggatgtt tcaggaatca tgatggctga tccgcgaatc
     6301 atcgatcatc ctaaaatcat cgaggaaatc tcttttagag aactgagaga gatggcttat
     6361 atgggggcta acgttcttca tgaagcagct gttttcccag ttcaagaggc tgatatcccc
     6421 atccaaataa aaaataccaa tgatcctttc gctcaaggga caaaaatatc gaatcacgaa
     6481 atagaaaagg aaaatggact gacaggaata gctggacgca aggattttct ttccatcact
     6541 ttattcaaac gtcatatgtc cgatgaaatc gggtttatct ggaaagcaat gagcattttt
     6601 gctaaacatg agatatcaat cgaacatatt ccttcaggga tcgacaatat cggagtgatc
     6661 gtctcagcag aagcgattgc cgataagtta tttctcatca cgaaagaatt aaaagaaaca
     6721 cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt tgatctctgt cgttgggggt
     6781 ccgcataaag aattgattgg attatctggg aaagtgttat cgatattgaa caaattagaa
     6841 attcgcacat cgatcttatc acaaggagca caggagttga atctgatcat cggtgtaccg
     6901 aataatcaat atgaaacagt agtgaagggt atctatgaag ggatggtgaa tacggatgct
     6961 gaaagaatat cgaatggcac tccatcaaat tcctgaaatc gggtttcatg aattcaagac
     7021 aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc gttatccatg aaatcggcga
     7081 aacaggacta ctcctttatt ttgataatca gcaagaagaa acgattgctt ttcgaacaga
     7141 tatcgatgca ctgccagtca aagaagaaac gaatctctct tttgcttcta agcatgaagg
     7201 ttatatgcat gcatgtggtc atgacggaca tatggcgatg cttttagggt tgactgactt
     7261 tataactaaa cacattgagg atcttattta taatgtagta ctgatctatc agccatcaga
     7321 agaaatttcc ggtggcgcac aaagcgttat tcagtcagga ttgttggata aatatgaagt
     7381 acaagcaata tttggctttc atctatggcc tggactagct aagggagaag ttttctctcg
     7441 accaggacct ttgatggcac aaagcagtga aacagacatt atcattaaag gccagtctgc
     7501 acatattgct tctagtgaaa aaggaatcga tagtctggag gcagctgtac gatttttgaa
     7561 gaatgtttat gattttgacc ggcaattacc ggcagagtgg aatcatctaa tgaaatttgg
     7621 ccaaatccaa ggtggaacga tccgaaatgt tttagctgga gaagttattg tatccggaag
     7681 tatccgttct tacagttcag atatgcaaaa ttatttgaag aggggattag accatttagc
     7741 agaacagttt cactctacag ctactatttc ttttcgctat aatgacggct atccagctgt
     7801 aatcaaccat agaggcttat atgcagcact gttagattca aacaaactac atgaattaga
     7861 tgctccagtt cttcaagccg aagattttgg catatatacg caacaatatc catgtgtatt
     7921 tttcttttta ggtgtcggtg atacccctgc tttacatgat ccgacgttcg attttgatat
     7981 ggatgtttta gaaaatggtg tagaatggta caaaacgatt ctttttacag aaaatcttct
     8041 tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat aatgatgaat ttatccaatc
     8101 gatttagtcc taggctttct agaatagaag tatcaaaaat acgacaattc gatcagcaaa
     8161 tatcgtcgat accagatgtg atcaaactca ccctgggaga accggatttt ccaactcctg
     8221 aacatgtcaa gcaagcagga atagcagcga ttgaagaaga cttttctcac tataccggaa
     8281 tgagaggatt agaggaactg cgggaagcgg cttgtatatt tcagcaacag cgttatggat
     8341 taacttatga tccgcaaacg gaagttttga cgacagttgg tgcaacggaa gcgattgcct
     8401 ctgcgttgtt atctgttttg gaagaaggag acaaggttct tattccagcg cctgcttatt
     8461 caggatatca gccattagtg gaactagctg gtgctgaatt gatcccgata gatacttctg
     8521 acacagggtt tgtctgtcag ccggaacaat ttgaacgagc atttgaacaa tatggatcag
     8581 ctgtcaaagc agtcatttta aactatccta acaatcctac tgggacgaca ttgtctgcaa
     8641 aacaattgaa agcaatcgct gaggttttaa aaaaatatcc ggtatttgtt ataagtgatg
     8701 aagtatatgc tgaattgact tatagtggaa cgcatatgtc catagctact tatcttccag
     8761 aacaaacaat cgtgataagc gggttatcca agtcacatgc gatgactggt tggcgagtgg
     8821 gctttatttt tgctcaaaaa gcccttattg acgaactaat taaagtccat caatatctgg
     8881 tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga agcgctaata aatggggtga
     8941 aagatagcga gaatatgaaa gagcaatatc aaacacgaag agactatctg gtccagcaat
     9001 tggcgccttt aggatttgaa gtgacccagc caaacggtgc attttacttg ttttgcaaac
     9061 tcccagctgc cattcaaact gactcttggc agttttgtgt agaattagcg gaacaggcaa
     9121 aagttgcgtg tatcccagga attgcatttg gcccagaagg agaaggttat atccggattt
     9181 cttatgctag cagcatggaa aaattacatg aggcctgcaa acggatcaaa gactttttaa
     9241 cagaacagtg attcatagat tttatcaaaa aaacaatcgt taattttgcg gtatattctg
     9301 caaggagacg attgtttttt aaatttagtt caaatgaacg cgtttctttt gattcataac
     9361 taatgtaatg ttaagtttaa attgtttaga atgattttaa aagaatattc aaggaggttt
     9421 catcaatgga aaaaatcaaa gttgtcattg taggtgcatc tcatggaggg catcaatcga
     9481 tcttggaact attgacacga tacggtgaaa atgtagatat cacgctattt gaagcaggag
     9541 actatatttc cttcatgtca tgtggtatgg agctgtattt agaagacaag gtaacagatg
     9601 taaatgatgt acgtaatttc agaccagaaa actttccgca atccaatgtt catattttaa
     9661 ataatcacga agtaaaaacg atcgatgccg ataaaaaaac agtaacggtt actagcgcat
     9721 cagataacga aacaaaagaa tatccttatg acaaattgat tttgagttct ggtgtcaaac
     9781 caaacaatct gccagtacca ggagcagatc tagaaaatgt ctacttgatg cgtggataca
     9841 attgggcaac caaaatcaaa gaaaaattaa atgatccggc tattaaaaaa atcgctgtcg
     9901 taggtgcggg ttacattggg atcgaagcag cagaagcaag ccgaaaagct gggaaagaag
     9961 tcgtattgct tgatgtgatc gatagacctt taggcactta tttagatgct gagatgacag
    10021 atattttaga acaacaccta aatgaaaacg gtattgaagt tttgacaaat gcaaaaatcg
    10081 aagcttttac tggtaacgga aaagttgagg caatcaaaac aagtgaaaaa gaaatcccag
    10141 cagatctagt cattcaagct gccggtgtga aggcaaatac agaatggtta aaagggatcg
    10201 tcgacttaga tgaacgtggc tggatccaaa caaatgaata cttgcaaacg aatcttccag
    10261 atgtgtatgc agtaggagat gctacacttg cttattctat cccagcacgc aaaaaaatgc
    10321 cgatcgcttt agcgactgtt gcacgtagag aagcacgtta tgtagtaaaa catctttttg
    10381 aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc ctctgcatta agtgtctttg
    10441 actatcattt tgcagcaagc ggattgaata gctttacagc agcaaaagca ggagtagatg
    10501 tacgagtgtc ttattatgaa gatacgttac gaccaaaata tgtaccagaa gaacaaggaa
    10561 atccaaaagt gtccatccaa ttattctcgg atccaatgac ccatcaattg ctaggaggag
    10621 cagtattatc tacctatgat gttacaggtc aaggaaatgt attggcacta gcgatccaaa
    10681 acaaactgac tttagaagaa ctagcagaag ctgacttctt cttccagcca ggctttgacc
    10741 gacaatggag catgttgaat ctagccgctc aacaagcgtt aggagaagaa ccatttgtag
    10801 aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag ctgccttgca tcaagtaata
    10861 agaacagcca agtaaaaaca gcttcttatg tttttaactt ggctggactt attaccgcag
    10921 atgcaagctt ttgaacaccg tttattttca aaaaagggac tgtgacacga tttgtgtacc
    10981 gacccccaaa agttagacca aaaaatctaa cttttggagg tcggtatttt ttatggttaa
    11041 atatactttt gaacttaaat taaagatcgt tcatgattat ttggatggca aaggcgattg
    11101 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaaattcg ggagaagcac
    11161 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
    11221 aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa aagtaacggc atacacgcgt
    11281 aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DAZUKA010000092        10973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000092 DAZUKA010000000
VERSION     DAZUKA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.095"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..1704)
                     /locus_tag="ACG8PN_001964"
     CDS             complement(124..1704)
                     /locus_tag="ACG8PN_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4862101.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2105..3481)
                     /locus_tag="ACG8PN_001965"
     CDS             complement(2105..3481)
                     /locus_tag="ACG8PN_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4862102.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3609..4697)
                     /locus_tag="ACG8PN_001966"
     CDS             complement(3609..4697)
                     /locus_tag="ACG8PN_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4862103.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4869..5303
                     /locus_tag="ACG8PN_001967"
     CDS             4869..5303
                     /locus_tag="ACG8PN_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862104.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5381..5914)
                     /locus_tag="ACG8PN_001968"
     CDS             complement(5381..5914)
                     /locus_tag="ACG8PN_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4862105.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6008..7186)
                     /gene="mutY"
                     /locus_tag="ACG8PN_001969"
     CDS             complement(6008..7186)
                     /gene="mutY"
                     /locus_tag="ACG8PN_001969"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4862106.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7179..7976)
                     /gene="recX"
                     /locus_tag="ACG8PN_001970"
     CDS             complement(7179..7976)
                     /gene="recX"
                     /locus_tag="ACG8PN_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4862107.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8083..9465
                     /gene="rlmD"
                     /locus_tag="ACG8PN_001971"
     CDS             8083..9465
                     /gene="rlmD"
                     /locus_tag="ACG8PN_001971"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4862108.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9639..10217)
                     /locus_tag="ACG8PN_001972"
     CDS             complement(9639..10217)
                     /locus_tag="ACG8PN_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4862109.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10539..>10973
                     /locus_tag="ACG8PN_001973"
     CDS             10539..>10973
                     /locus_tag="ACG8PN_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4862110.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFA"
BASE COUNT         3262 a         2207 c         1897 g         3607 t
ORIGIN      
        1 attttaatat agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg
       61 agaggctgtt tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact
      121 tctttacatc aaacttttta attttacatt gggatattta tccgcaaacc agcgttctgc
      181 aaattggttc tcgaataaga acaacggttg gtcgaaacgg tcacgagcca agatattgcg
      241 acttgaactc atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc
      301 cattggagac atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc
      361 gaattgtagc tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag
      421 ctgaatcgct ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat
      481 gacatttttt gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata
      541 agcaactttt agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata
      601 tacaccgatg atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa
      661 ttgtgtgaca ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc
      721 tcgttcgaat gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt
      781 catatttgct tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat
      841 ctgatcatct tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa
      901 aaaagtctgt acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc
      961 acgagcaatt ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc
     1021 taagacttgt tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc
     1081 ttcatccaat tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg
     1141 attatggtag atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat
     1201 cggataagat tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg
     1261 accatcacga tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac
     1321 ttggaataat ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc
     1381 actatccacg gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt
     1441 atccaagata tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga
     1501 aatcccacgt tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt
     1561 gccttttacg gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat
     1621 cgttgttttc ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg
     1681 ttgctttaat tttggattat tcataaccta actccatttc ttccattcaa ttatttatat
     1741 tttctctagg cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga
     1801 caaaagccgt gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag
     1861 caataagccc acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac
     1921 ttaatgcagg atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca
     1981 tggattcagc gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg
     2041 actaaaagaa aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt
     2101 ccaattatct tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt
     2161 caggttccgc ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag
     2221 aaccttccat ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc
     2281 cttcatccgg aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac
     2341 tcatatgcag atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga
     2401 tgatatattc gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat
     2461 cgatttctcc gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc
     2521 catattcatc taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat
     2581 catcaataaa gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta
     2641 aattatcaaa gccgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga
     2701 ctactttatc tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt
     2761 cattcacatt ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga
     2821 ccatgacttc tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt
     2881 cttcattatt caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt
     2941 ttgaatcttc atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag
     3001 aaagcagcca gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga
     3061 actttgcgac ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa
     3121 caatcgatac ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg
     3181 tgccgccacc taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag
     3241 acaaaatgtt gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct
     3301 gcaatatttt taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc
     3361 gattcttatt gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg
     3421 tcaaaacgat caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca
     3481 taatcgcatt ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac
     3541 tatagccacg ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact
     3601 aaaactacct acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg
     3661 acgattttga ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag
     3721 aagataccaa gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat
     3781 ggatggattt tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt
     3841 tggacgatta gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt
     3901 actagaaatg caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat
     3961 cccgcaatga ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat
     4021 gtgaaggtac caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt
     4081 ttattcagtt gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt
     4141 tggatgtttg gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct
     4201 gtaatgatta ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat
     4261 actccactaa ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat
     4321 ttattgatat ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct
     4381 acctgtttga tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt
     4441 gggatgacac taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg
     4501 acagcccata ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa
     4561 tagtaaagaa agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc
     4621 ggttcgaaaa taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat
     4681 tcaagcgacc aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct
     4741 ttctttctta ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt
     4801 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
     4861 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
     4921 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
     4981 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
     5041 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
     5101 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
     5161 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
     5221 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
     5281 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
     5341 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
     5401 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
     5461 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
     5521 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
     5581 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
     5641 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
     5701 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
     5761 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
     5821 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
     5881 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
     5941 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
     6001 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
     6061 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
     6121 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
     6181 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
     6241 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
     6301 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
     6361 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
     6421 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
     6481 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
     6541 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
     6601 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
     6661 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
     6721 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
     6781 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
     6841 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
     6901 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
     6961 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
     7021 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
     7081 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
     7141 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
     7201 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
     7261 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
     7321 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
     7381 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
     7441 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
     7501 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
     7561 ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
     7621 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
     7681 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
     7741 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
     7801 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
     7861 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
     7921 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
     7981 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
     8041 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
     8101 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
     8161 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
     8221 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
     8281 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
     8341 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
     8401 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
     8461 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
     8521 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
     8581 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
     8641 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
     8701 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
     8761 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
     8821 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
     8881 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
     8941 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
     9001 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
     9061 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
     9121 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
     9181 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
     9241 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
     9301 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
     9361 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
     9421 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
     9481 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
     9541 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
     9601 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
     9661 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
     9721 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
     9781 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
     9841 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
     9901 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
     9961 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
    10021 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
    10081 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
    10141 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
    10201 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
    10261 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
    10321 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
    10381 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
    10441 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
    10501 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
    10561 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
    10621 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
    10681 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
    10741 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
    10801 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
    10861 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
    10921 atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc gca
//
LOCUS       DAZUKA010000093        10860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000093 DAZUKA010000000
VERSION     DAZUKA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.096"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..659
                     /locus_tag="ACG8PN_001974"
     CDS             81..659
                     /locus_tag="ACG8PN_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4862111.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            659..1588
                     /locus_tag="ACG8PN_001975"
     CDS             659..1588
                     /locus_tag="ACG8PN_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862112.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1585..3321
                     /locus_tag="ACG8PN_001976"
     CDS             1585..3321
                     /locus_tag="ACG8PN_001976"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4862113.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3384..4220)
                     /gene="pheA"
                     /locus_tag="ACG8PN_001977"
     CDS             complement(3384..4220)
                     /gene="pheA"
                     /locus_tag="ACG8PN_001977"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4862114.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4217..4726)
                     /locus_tag="ACG8PN_001978"
     CDS             complement(4217..4726)
                     /locus_tag="ACG8PN_001978"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4862115.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4719..6014)
                     /gene="aroA"
                     /locus_tag="ACG8PN_001979"
     CDS             complement(4719..6014)
                     /gene="aroA"
                     /locus_tag="ACG8PN_001979"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4862116.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6027..7118)
                     /locus_tag="ACG8PN_001980"
     CDS             complement(6027..7118)
                     /locus_tag="ACG8PN_001980"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4862117.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7135..8301)
                     /gene="aroC"
                     /locus_tag="ACG8PN_001981"
     CDS             complement(7135..8301)
                     /gene="aroC"
                     /locus_tag="ACG8PN_001981"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4862118.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8303..9367)
                     /gene="aroB"
                     /locus_tag="ACG8PN_001982"
     CDS             complement(8303..9367)
                     /gene="aroB"
                     /locus_tag="ACG8PN_001982"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4862119.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9400..10422)
                     /gene="aroF"
                     /locus_tag="ACG8PN_001983"
     CDS             complement(9400..10422)
                     /gene="aroF"
                     /locus_tag="ACG8PN_001983"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4862120.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3016 a         2396 c         1997 g         3451 t
ORIGIN      
        1 taaaataaaa atatccttgc attttctcct agaaataagt atttttaaat tacaagacta
       61 ttcttaaggc gggtaaaaaa atgccaatac gcgtaattct tatcgatgat gaacagacga
      121 ttttagatgg aatgaaacag ttgatcaatt ggtctgagca cgggtttcaa ttaataggga
      181 ctttttccag tccgctgaaa gcgcttgatt tttctttggt acatgcaatc gatatcgtgg
      241 tcacagatgt aatgatgcct gaattaaatg gaatcgaatt atcgcggcta ttaaaacagc
      301 aacaacctga aacacagatc cttatcctct ctagctatga tgaatttgat atggttaaag
      361 attcttttaa agaaggcgtc gctgattatc ttttaaagcc tcgattgagt cctgattttt
      421 ttctaaattc cttaactgcc gtttcacaaa aaatcaaaaa aaagcccaac aaaagcccaa
      481 tcaccaatcg ttatgagcag atctgcgaga atctttcaca gcagattgct ggacaaactc
      541 gtccattgct tgaccctctt ctttttgaac acaccttgtt tttctgttat ataccgaaaa
      601 acggccattc agttcaaaac aaggctctaa acaaaaacag tcgatcaccc attcattgat
      661 ggatagcgac gattcgttta ctatttatcc tgtttctacc ttttcggaag aatccggcta
      721 tgtaatcaat actgattcca aagatacact taggaagttt gttcaaaagt ttcgtcaaga
      781 agtttttcca ggtgattttt ttgttgtttc tgattctttt gcaatccaag atatcaatga
      841 aatattccaa gagatcagaa attgcaccaa aggacaacgt ttctatcata agggaaagaa
      901 ccttgcttat cagcatgaac ttcttccgtt attaaccaac cacgagcagc aagcaagtag
      961 ttatttaaca aaaatcgttc ataaagacta cgctttagct gtcaaagaac ttgagaattt
     1021 cttcacgacg atattggaac agcctattga tccagcttat ttgaaacatg aggcaatcaa
     1081 tcgtttctat gcattactca acgctttggc tgacgagcat gttgggaatg aagaactttc
     1141 tttgctcaag atcgcccttc cttctttatt agccgatgct gagtatttcg aagatttttc
     1201 tattcttgtc aaagaaacac ttgagaaact cacccacttt tctgcaaatg caaaaaaaga
     1261 caatttcgat atcttgactt tgatctacga gttcgtccag gaaaattatg agaaagaaat
     1321 caatttacaa cttttatccg aaaaatatca tttttcatat agctatctct cagccatctt
     1381 tactgaaaaa tttggtatca acttttcaaa atatctaaaa aaagtccgta tcagtaaagc
     1441 caagctcttt ttgacggaaa cacggcttac tttgacggaa atctgtgaga aagtcggcta
     1501 taccgaactg ggttatttct ccagagtttt taaagaagag acaggaatga caccttctca
     1561 ataccgtaaa ggaacttttg taaaatgaga aaaaaaatca aagagcatga actctttgcc
     1621 aaacttctgt tcattttgtt catcggtctt tttctgcaag ccatcgtcat ttctttgttt
     1681 atttatcatc gatcaaggga tgcctatatc cagctgttca atcaatcgaa tgatgtggta
     1741 ctaaaaaaga ttcaatctga atttgaaagt ttgaatgata caatcgagaa tacattagcc
     1801 gcttttgatt ctaatccagc tgtcaaaagc tatttttcca atgatccagc ccaacatatg
     1861 gaacagcttc agcaactaag aacaatccaa aaaatgaatg actctctttc aaaaatccat
     1921 ccaatgatcg attacgatgt attgatcttt ggagaaaatg gaagaacttt tgtcggaaac
     1981 gacatgctca ctgccgtatc tgccgattct ttttttcaat ctgcgattgc tcagagagtg
     2041 aatgaacgtg ctgctgatac acaaatgctt tttgcacacc acggattgac tcttcgcgat
     2101 aagaaagcac ccagcgtctt ctttgtcaaa aaactgaaaa atacattgaa tcacgtttac
     2161 ggatatgctg ttttatcgat ctcatccaag caactagcga atctttttca atctgtagta
     2221 aatcctgaga tatcgaagat ctccttcatg aacgaagaac agacgatcat cgcatcaaat
     2281 gaagaaaata caattgggag ccgatcaatg attttcaaaa aactaacagt aggtgaaaca
     2341 acgatttcaa atggacagca gttgacccgt cttcctctgt accgccagga ttgtacatta
     2401 ctcagtcaag tcgatcttca ttctttatct aaccaaatgg gcgtgatttt tccgattatt
     2461 ttgtataata tcgtgacact tgcttttgct ggctcattgg tatttatgta tttggatcga
     2521 cacacaaaat cgatctaccg cttgattgat tcgctaaaac ggctagagga agatggctta
     2581 aaagctactg ttcctgtaca aggaagccat gaagtccaat tgctagggaa tacgatcaat
     2641 caattgctaa gtaatgctca gcatcattat gaacagacga tccaagacga aaagaagaaa
     2701 cgttcgcttg aaatccaaac tatgcaagca cagatacagc cccattttat ttataatacg
     2761 ctgactagct tgaaattttt gatttggcag aaaaaaacag atgaagcagt tcaaggaatc
     2821 gacagtttca ttactttact aagaagtacg attgggaaaa aagaagaagt gattccagtc
     2881 aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt tgagtatgcg atatggcgaa
     2941 agaatctcgg ctaaaataat gattcctgat gaactgcagt cattatctat gccgaatatg
     3001 atccttcaac caattatcga aaacgcctat cttcatgctt ttcacaataa aacgcaagga
     3061 ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt tattcgaagt gattgataac
     3121 ggcgatggct tcgatactga aaataccaaa caaaaaaatg atcatttttc aggaatcggc
     3181 atcagccatg tagatgagcg gatacgccta atgtatggcg atgcatatgg cttaacgatc
     3241 caatcagtga ttgggacagg aacgactgta acgatttgtc taccgcttct tcctaaaaat
     3301 caatcagaat atcaaaaata aagaatagcc aagcgctgat ttttttattt taagttaaat
     3361 aaatcaatca gtattcttga ctttcatcta cgcgtctctt ctactgtcag cactggataa
     3421 cagcccaata tttgactttc tcctccgagc atcttgattt cttggagcgc attctctact
     3481 aatgtcattg gacggtccaa caacaaatcg atcacgaaaa aatattctcc caagctagtt
     3541 ttcaatggtc gggattcaat cttacttaag ttgatttccc gccagccaaa agctgccagc
     3601 attttatgaa gcatccctgg ccgattagca ggtagtgtca agatgacact catttttact
     3661 ggagctgact gctggctggt catctttcgg tcaccgacga tccaaaaacg tgtctggttc
     3721 aactcattat cttggatatt ctcagctaag atctccaatc caacatgctg tgctgtttct
     3781 aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg ctacatacat tgctgcagca
     3841 gttgttgatt ctgttgctac taaaggaaca ttaggataat gcgtttctaa aaactgttgt
     3901 gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa tctttgttgc tggattgcct
     3961 aaaagctgct gcttgatagg aagaacgatt tcacttttaa cttcgacttc tggatgtttg
     4021 gaaagaagat cgattgtgtg atgaacagct ccttccaatg aattctctac aggaacgaca
     4081 gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa cagggatgga agcatacgca
     4141 gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat aagtgaatga actttctggc
     4201 cctagatagc tgacgttcat atgcttttga tcctttctat aatggtatcg atgatctttt
     4261 ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact ttcttcatac caagaaagtc
     4321 gttgtgccaa taatgctttt atttcttctc ttgtttttcc aagagctaaa ggtcgaatag
     4381 acgtttcatc ttgttggata cgatcaacta gacagtccgc ttctgcctct aagtaaagaa
     4441 caatcggctg tgtttttaaa aaacgccgat tttctgcact ttggacaatc cctccaccag
     4501 tcgcgataat tttgtcttca tttgacagct ttcttaaaat atctgactcc acttttcgaa
     4561 agcttttttc accgtaatag tgaaaatatt cggcaatcga caattggata tgttcctcaa
     4621 tctttgtatc taaatctaca tactcttttt gtaaacattg agctaaccct ttaccgattg
     4681 ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct agccatgagc ttctcctcct
     4741 gacacaagtt ctgctaaatc gtcaaaaaat gccggataag agattgaaac agcttcagct
     4801 ccttctagtt cgcaaggtcc atcagctatc aaagcagcta cttgcagcat cataccgatt
     4861 cgatgatcgc ctctgctgga cacttttgcg ccatgcagag gtgtcggccc atggatgatc
     4921 agaccgtctg ctgtcgcttt tatctttgct cccattttct gtaattcttc tgccaccgca
     4981 tcaattctgt tggtttcttt tacttttaat tcttctgcgt ctttgatgat cgtgattcct
     5041 tctgcttgag tagccactaa agctagaatg ggcaattcat caatcagacg cggaatgatt
     5101 tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga cacttaaatc agccgattgg
     5161 ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca tctcctccaa cacatctaag
     5221 ataccagttc ttgttggatt gaccccgaca ttttttaaca acagttggct ttccggaaca
     5281 agaagtcctg ctgctagaaa gaaagcagct gatgaaatat cacctggaac agttatctgt
     5341 tgaccagtta atttttgcgg tccggtcact atgatcgttt tatcttccac tgtgatcctt
     5401 ccgccaaatt gacgaatcat ttcttccgta tggttacggg aacgttcttt ttcaacgatt
     5461 acggatgtgc cttctgcttg taatgccgca aacagcaaag cagatttgac ttgcgcactg
     5521 gctacaggca tattgtatcg gataggtgat aaagagcggg ttcctgtcac agtgattggt
     5581 agatactggt cattttcttc tccttgcaag tcagcgccca tctcgcgtaa aggttccatc
     5641 actcgcccca ttggacgttt agacaacgaa gcatccccga acagagtagt cgtaaaaggc
     5701 tgtccagcca aaatacccat caataaacgt atggtcgtac cggagtttcc catgtcaatt
     5761 gattcggttg gttggacaaa tgagtggcta ccgcgaccat gaatcttgat cacctcttcc
     5821 tcttcttcga tctcgacacc taattgttgg aaggcatgta aagtactcag acaatcttct
     5881 gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa ttgctccaaa catgatgctg
     5941 cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc ctcttaatcc atgtatttgt
     6001 tgtaataatt gcatttatcc agcctcctat ttgatccagc aatgataact ggtattagac
     6061 ataatgattc tttttgcttg taacaaatcc tgttggtttt taaaactgat ttgcagtatt
     6121 cctaaaatat cttccctggt ctcttggatc ttcaaattaa tcaaagaaac tcccgctttt
     6181 ccgatcagtc cagttacttc tgcaataacg ccaggttcat ctggcacatc gataaataaa
     6241 tcaaaaaagg caggaatcgc gccttgctta tggacaggca actggtctcg actttctttt
     6301 gcccgattga aaaaagaaaa gatttccgat tggttctcag tcatgatcca ttctgctatt
     6361 tgtgtcattt cttgttgcca atctgccaat cgttccaata atatctgctt attgctcaac
     6421 aaaatatctg tccacatgac aggatctgag gaagcgatcc tagtgatatc tcgaaagcct
     6481 cctgctgcca gttgtttggc tcgcggatac tcttgattga attgttctgc ctgattgact
     6541 aagccagctg ccagaatatg tggaaaatga cttagcattc ctgttatacg gtcatgttct
     6601 cttgctgtaa gagttacata ttttgctttg gttccgcaaa atagttcttg cagtttttca
     6661 acatcttttt tcgtcttttc caaaggtgta aagatgtaat aggcattttc aaatagttta
     6721 gcatctgctg cctgtatacc tgatttatga gaaccagcca ttggatgtcc accgacaaac
     6781 cgaagatttt tctgctcagc ggatgtcacg acttgctgct tcgtactgct gacatccgta
     6841 atgatgacgt gatcagacaa gttgattctt tcaaggacat caagataagc taaggttgta
     6901 cggacgggaa cggctaataa gataacgtct gcttgttctg cacctgtttc aaaatcactt
     6961 gcaacgtaat caacgatctg ctgttctact gcttgctcac gggtttttga ctgactatcc
     7021 caaccaacta ctatcagttc tggattggtc tgacgaatac aaagtgccat agaagcacca
     7081 atcagtccaa gccccacgat aaaaacatgt tgtgccatct tatttgcccc ctttttagta
     7141 atgtttcaag tagtcacggt actccttcac tgcagctttc aactcaacaa gcgaatcact
     7201 tgggaatttt gacaatagct ctgtcgctac cacagtggct acaacatttt ccgcaacgac
     7261 gctagctgca ggaactgctg tactatccga acgttctaca cttgctttgt aaggttcttt
     7321 tgtatcgata tcaacacttt gaagtggctt gtacaatgta ggaatgggtt tcatgacccc
     7381 gcgaatgaca agtggttcgc cattggtcat tccgccttcg aagccgccta gattattggt
     7441 acgtcggaaa tagcctcttt cttcttccca taggatctca tccatcactt ctgagcctgg
     7501 taatgaacca gaagtgaatc cgaccccaaa ttctacgcct ttaaaggcat tgatactaac
     7561 cacagcttgt gcgattttgg catctagctt cgtgtcccat tgtacgtagc ttcctaatcc
     7621 agctggaacg cctccaacga gcacttctac aattcctccg atcgtatctc catttttctt
     7681 tgtctgatcg atcaaatctt ttattctttg ctcaactgct ggatcaacaa ctcgtacttc
     7741 tgacgcttca gaaatttctt tgatttcttg aatcgagata tttccaggta tttctgcttg
     7801 gattccacca agacaaatca cgtgtcctgc tacgatgatg tcaagttctt ccagaatttt
     7861 cttggcaatt gcgccgattg ctacacgcat ggtcgtctct cttgcagagg accgttcaag
     7921 cacattccga agatctcgat gatggtattt cataccgcct accaaatccg cgtgtcctgg
     7981 acgcggacgt gcaactcgcc gcagtgattg gtctttttca ggcacttctg ctacagacat
     8041 gacagttgtc caatttttcc aatctttatt ttctaccact aaagtgacag gtgaaccaat
     8101 tgttttgccg tgacggatgc cagacgtgat ccgaacacga tctttttcga tcttcatcct
     8161 tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg tgattgattt cttcttctga
     8221 cagaggtaat cctgctggta gtccttcaat aatggctgtt aattctggtc catgggattc
     8281 tcccgcagtc atataacgca tcttacagtc ctcctaagta atctttgaat gtctctgtgg
     8341 gaacaggaac gatttttgct tgcccaatct tttctagcaa gatgatattc aattggcttc
     8401 ctcttgcttt cttatcatgg gttatcgcgt ggaataattc atctggatga cttagatcgt
     8461 acgtaatcgg cagatggaat ttttgaagta tcctttctaa agcagctgtc gatccagttg
     8521 gagacaagcc gattttttct gcatgagaag tgatggctac cattccaata gctactcctt
     8581 ctccatgact gactacccca tatcctgctg tattttcaat tgcatggcca attgtatgac
     8641 caaaatttaa aataagtcga tttccttggt caaagacatc ttcttccact acttttcgtt
     8701 tgatttccaa cgtagcagta atgattggaa cagcatgttc cagaagatct ttttcgtctt
     8761 tcagctcgtt cagctgtttc caaagcgttg catctgctat agctgctgat ttgatgattt
     8821 cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt tagaacctca ggatcaatca
     8881 atacaccagc tggctggcta aatgttccga caaggttttt cgctttgttt gtattgactg
     8941 ctgtcttccc gccaatacta gaatctactt gtgcaagtag cgtagtcgga atttgaacga
     9001 aatggatccc acgcatgaac gtcgaggcca cgaatgctgc tagatcaccc accattcctc
     9061 caccaagtgc aagtatccca tcgctgcggg taaaatcatt atctgctaaa aaatcgtata
     9121 actcagctgc ttgttctaaa gatttactgg tctcacccgc cggtacgatt gaaaggactg
     9181 gttcaaaatt agcttgtctc aattggaaca tgattttttc tgcatatagt ggagcaacgt
     9241 ttgtatcagt gatcacagct actttttgag gtctccaaat cgtttgaagc catgatccag
     9301 cctgttctag tgcgcctttt tcaatatgaa tatcgtattt ttggttatca acatgtacag
     9361 taagcattcg tttcacttct ttcttttaaa aagctggaat taatcgagtg tctgaatctt
     9421 ttccattgct tctttcatga tcgcaacttc tttcatcatg cgtgagtaag tcttttcatt
     9481 caacgattgg ggaccgtctg accaagcatt tgcaggatca ggatggatct ctacgatcat
     9541 gccatctgct ccgcttgcca cacccgcacg agccattggt ggtacaagtt cccaaattcc
     9601 tgtaccatga cttggatcaa caatcacagg gaaatgagtt aattttttca tcaatgggac
     9661 agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa gtccgaatcc cacgttcgat
     9721 aaaaatgaca ggagatttgt cttcgactgc aatgtattct gcagcattaa gccattcatt
     9781 gatcgtaccg gaaattccac gtttcaatcc aacaggttta cctgttttac cgacagcaga
     9841 tagcagttta aaattttgca tattacgtgc gccaatctgt aggatatcgc tgtactcagc
     9901 caccatatcg atatgccctt catccatcac ttcagtaatc actttcattc caaattcatc
     9961 agccgcttga cggatgtatt tcaatccttc ttcttctaaa ccttggaaag catatggaga
    10021 agtacgtggt ttgaaggcac cacctcgcag aatttttgcg cccccggctt tggccattcg
    10081 cgcacattct ctgatttggt ctaatccttc gatagaacaa ggaccagcca tcgtcacgaa
    10141 actgccgtca ccaattttta ctccgtctac atccacgact gtattgccag gatggaattc
    10201 tcgagaagtc aatttgtatg tgtttaaaat acgcacagca ttttctactc cttcatagcg
    10261 tgtaaatgct ccttcttgta aacttcgcgt atcacctttt agtccaatca ctactcgttc
    10321 tgttccttga tcgattgaga ctttgaatcc ttctttttcg atatgatcga cgactttttt
    10381 tatttgttct tccgttgctt cttttttcat aattactatc attgtttttc ccgctttctt
    10441 ttgtttactt atctatcttg gctgattttc tctacttttt caaggtggtt ttacattaat
    10501 tcctactaaa tgaccgtgtc ctgttgttct cattttcgct ccttctttcc tacttatgac
    10561 ccagataatc aacaaaaaaa gatgtccctg taggaaattg cctctacagt gaacatcttc
    10621 aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca ctatagatga atttctctac
    10681 gtggctgtaa gtcgtgttta aaaatcttta gccatcatag atacaaacct ggtttgtttg
    10741 tacccacgaa gtcatgaata caaacctatc taaaatagct aaagtaatac gtattcagtt
    10801 ttgtaagttt tctagatttc ataaaaaatc gctccaaact gatgtaagaa ttgtacctag
//
LOCUS       DAZUKA010000094        10616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000094 DAZUKA010000000
VERSION     DAZUKA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.097"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8PN_001984"
     CDS             33..656
                     /locus_tag="ACG8PN_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862121.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8PN_001985"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8PN_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4862122.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8PN_001986"
     CDS             2152..3033
                     /locus_tag="ACG8PN_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862123.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8PN_001987"
     CDS             3036..3806
                     /locus_tag="ACG8PN_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862124.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8PN_001988"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8PN_001988"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4862125.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8PN_001989"
     CDS             5117..6280
                     /locus_tag="ACG8PN_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4862126.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8PN_001990"
     CDS             6397..6807
                     /locus_tag="ACG8PN_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4862127.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8PN_001991"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8PN_001991"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4862128.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8PN_001992"
     CDS             9380..9664
                     /locus_tag="ACG8PN_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862129.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8PN_001993"
     CDS             9824..10060
                     /locus_tag="ACG8PN_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4862130.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8PN_001994"
     CDS             10057..10455
                     /locus_tag="ACG8PN_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4862131.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3712 a         1771 c         2242 g         2891 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatag
//
LOCUS       DAZUKA010000095        10574 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000095 DAZUKA010000000
VERSION     DAZUKA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.098"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..239
                     /locus_tag="ACG8PN_001995"
     CDS             15..239
                     /locus_tag="ACG8PN_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS4862132.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(444..764)
                     /locus_tag="ACG8PN_001996"
     CDS             complement(444..764)
                     /locus_tag="ACG8PN_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4862133.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(912..3146)
                     /locus_tag="ACG8PN_001997"
     CDS             complement(912..3146)
                     /locus_tag="ACG8PN_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862134.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3625..3891
                     /locus_tag="ACG8PN_001998"
     CDS             3625..3891
                     /locus_tag="ACG8PN_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4862135.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3891..5618
                     /gene="ptsP"
                     /locus_tag="ACG8PN_001999"
     CDS             3891..5618
                     /gene="ptsP"
                     /locus_tag="ACG8PN_001999"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4862136.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5782..6828
                     /locus_tag="ACG8PN_002000"
     CDS             5782..6828
                     /locus_tag="ACG8PN_002000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4862137.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6845..8068
                     /locus_tag="ACG8PN_002001"
     CDS             6845..8068
                     /locus_tag="ACG8PN_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4862138.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            8157..9044
                     /locus_tag="ACG8PN_002002"
     CDS             8157..9044
                     /locus_tag="ACG8PN_002002"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4862139.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            9130..9360
                     /locus_tag="ACG8PN_002003"
     CDS             9130..9360
                     /locus_tag="ACG8PN_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4862140.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9417..10382)
                     /locus_tag="ACG8PN_002004"
     CDS             complement(9417..10382)
                     /locus_tag="ACG8PN_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4862141.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3429 a         1840 c         2136 g         3169 t
ORIGIN      
        1 ggagagtgtg acacatggaa acagaatggc aaagacgtga aagaaaaaaa cagaaaaaag
       61 aagagatgtt acgattatgt cgattattag atgtaaaaga ctgggacaaa aacgagcaaa
      121 ttgcacaaaa agtacgatca atcatccata aagaggaaaa agttaaaaat aaagacacgc
      181 gtagaactga aaatgaaaag agaaaagtac aaaagaaaag agaaaaatca aaaagctaga
      241 aagaaaaaga ggctgagaca gaagcgttta actccaagaa ataagctgga attcacaaaa
      301 atttgaaaag caattttcgt gaatttcagc ttatttccga aggagttgct tctgctttcg
      361 ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt
      421 taattattat gttcaaaatt tgtttacacg gctaatgtag cgcctaattc atgttttgga
      481 atcgagattt caacaaggcc tgccaattcg attactttga tttcatcctc gtggtaaacg
      541 gcgtctttga ttttatctaa gaaataattt ttagcatctt cgatttcttt cggctggatt
      601 ttacgtttgt cattgatcag cagaatttca ttatgttcag gggcttcagt gtagacaata
      661 gtggtatttc cggctgtgta gaccttgact agatgggcat cagtgttttc aagctggttt
      721 aatacaagac gggaatggct gtttgttaca tttaccaatt tcatggcgct cacctctttt
      781 atgtattttt aattgcttgt ttctcttatt ataatttctt ttttataaaa tgaacagtat
      841 tttgactttt ctgaaaaaag aatgagacgg catttctcaa agaaataccg tctcacgcta
      901 taaaactgga tttattcagc cgtttcttcg gctgattctt tagccaaacg ttctggttta
      961 gaagtaacga tgatttttcc atctttatcc agttttgctt tcaactcatg gatagaaggg
     1021 tgatcaagat aaaattcagc gataccatct tcgatttgtt cttggatcgt gcgtcgaaga
     1081 ggacgagcac ccatagatgg atcatagcct aagtcaacta atttttcttt gacattcgta
     1141 ggaacttcga tgtgaagttg ctgtgctgcc agcatttggt tgacatcgtc tagcattagt
     1201 gtgacgattg tcatcaagtt ttcttttgat aatgcactga attcgatgat cccgtcaaaa
     1261 cggttcagaa attctggtgt aaagtagttg tttaattggt tcaatacaga acgagtaaca
     1321 ccttcgcgtg ccgcaccaaa accaacgttt gcttctactt taccagttcc ggcattactt
     1381 gtcataatga tgatcgtatc tttaaagctg accgttcttc cttgtgcatc tgtcaatcga
     1441 ccatcatcta agatttgcaa gaacatatgc aaaacatctg gatgagcttt ttctacttca
     1501 tctaacaaga caagactgta aggatttctc cgtacttttt ctgttaattg accagcttct
     1561 tcgtatccga cataacctgg aggagaacca atcaatttag agacactatg tttttccata
     1621 tattcagaca tatcgaaacg aatcattgaa tcttgtgaac cgaatagttc gtaggcaagt
     1681 tgtttcgcaa gctctgtttt acctacacca gttggtccaa caaataagaa ggagccgatt
     1741 ggacgattct gtttgtttaa tccaacacgg tttcggcgaa tcgctttcgc tactcgatct
     1801 acagcgttat cttgtccgat gacatgagct ttcaagtcat ctgctaagtt tttcagttgt
     1861 gtctgttctt tttcttttaa ttcacccact ggaatacctg tccgttgttc aacgattttt
     1921 tccatatctt tttcggtaat cacaggtgtt tcctcttcag tgagctgacg ttctttcatt
     1981 ttttgaagtt tattgatctg atcgcgataa taagctgctt tttcgaaatc ttcttctcga
     2041 gaagctaaaa ctttttgttc ttctgcttct tgcagttttt tctcgattgt tttaggatca
     2101 acgatttgga tcgtcaagtt tttcttagaa cctgtttcat ctagcaaatc gatggcttta
     2161 tccggcaaga agcgatcttg gacgtaacga ttggataaag ttgcggctgc ttcgatggcc
     2221 tcatcagtat attttacatg atgatagtct tcataacgtt tttgcaaacc tttcaaaata
     2281 ctgatcgttt cttcgacagt tggttcatct acccgtacag gttgcatacg acgttctaaa
     2341 gcagcgtctt tttcgatgat gcggtactca tttaaggtag tagcaccaac catttgcaat
     2401 tctcctcgag ctaaagctgg ttttaaaata ttaccagcat ccatatttcc atcaccagca
     2461 gaaccagcac cgacgatttc gtgaacttca tctatgaata ggatcacatt ttctgcttga
     2521 cggatctctt caatcaattt ttgcatacgt tcttcaaatt gtcctcgtat accagtacct
     2581 tggactaaag atacgacatc taaacgaatg acttctttgt ccattaattt ttgcggtaca
     2641 tcaccatcta cgattttttg tgctaaacct tcaacaactg ctgttttacc aacacctggt
     2701 tctccaatca agacaggatt gttcttggtt cggcgattta aaatttcaat gactcgtttg
     2761 atttcgtcat cgcgtccgat cacaggatcg atatcacctt gtcgtgcttg ttctgtgata
     2821 ttgattccgt attctcccaa taaaccttct gtattgcctt gaggtggctg accaccattg
     2881 aagttgttgt ttccgccaaa ttgtgttggt ggtgtttgct cataatttcc ttgctgttgc
     2941 atttggcgag acatggaacg gaaaagatcg tccaaactgc caaatccaaa tggatcattt
     3001 tgggccatat tagtcagacc tccattttgt tgatttttga ttttttgata acagctctga
     3061 cagtaatcga gttgtgtccg ctgaccattg acagtggcat acaagtgaat cgttgcctca
     3121 ttcttgccgc agttttgaca aagcatgaat cattcacgtc ctttcacaaa aatgggtaca
     3181 tccatatttt acatctcgtt tttcatgagg taaaagaata acccctagtt ttttaagaga
     3241 tttatgaggt agaaaaggtg aaatctctat tgaccattcc tgacctttga ctcttattat
     3301 actaaaaata aaaagggaag caagtaattc gtttcatatg aaaacaaaga gggtcaaagg
     3361 aaagaatgat aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata aacacgtttg
     3421 ttataaaatg tgaagaaaag gatgaaaaag aatccggaat aatttgaata cttttatctg
     3481 aaattgaatg aaaactgttg tatctaacga aaaaaaatgt taatctttac agatgaaagg
     3541 gtggcatttg ttaggattct ctgaattact aaaaaagaaa accctcacat aaaaacaaca
     3601 ctcacttaaa ggagaccgat taatatggaa aagaaagaat ttcacgtagt agcagaaact
     3661 gggatccacg ctcgtccagc tacactatta gtacaaacag caagcaaatt taactctgat
     3721 gtaaacttag agtataaagg taaatcagta aacttgaaat caatcatggg cgttatgtct
     3781 ttaggtgtag gtcaaggttc tgatgttact attactgcag aaggtgctga cgaagctgat
     3841 gcaatggcag caatcgttga aacaatgaag aaagaaggct tatctgaata atggttgaaa
     3901 tgctaaaagg gattgccgct agtgacggtg tagccgttgc aaaagcttac ctgctagttc
     3961 aaccggattt aacattcagt aaggcaacag tagaagatac tgcggctgaa gaagctcgtt
     4021 tggatgctgc attagctaaa tctactgaag aattgcagca aattcgtgaa aaagcagcgc
     4081 aaagcttagg tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt ctttcagatc
     4141 cagaaatggt aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat gctgaatcag
     4201 cgctaaaaga agtaacggat atgtatatcg gtatgttcga agcaatggaa gataatgctt
     4261 atatgcaaga gcgtgctgca gatattcgtg acgttgctaa acgtatttta gcgcacttat
     4321 taggcgttac tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt gttgcacatg
     4381 acttgactcc aagtgataca gcacaattag atcgcaattt tgtaaaagcg tttgtaacag
     4441 acattggtgg acgtacttca cattctgcta tcatggcacg ttctttagaa attccagcaa
     4501 tcgttgggac aaaagaaatt actgctaaag tcaaagaagg cgatattttg gctgtcaacg
     4561 ggatcgaagg cgatgtaatc atcgatccta cagatgaaca aaaagctgaa tttgaaaaag
     4621 caggtgcaga ttacgcagca caaaaagcag aatgggaaaa attaaaaaat gcagaaactg
     4681 tcacagctga cggcaagcat tttgaattag ctgcaaacat cggaacacca aaagatctag
     4741 ttggtgtcca taataatggt ggagaagcag ttggtctgta ccgtactgaa ttcctgtata
     4801 tggattctcc agacttccca acagaagatg accaatatga agcttacaaa gctgtattag
     4861 aaggtatgga aggaaaacct gtagttgttc gtacaatgga catcggtgga gataaagaac
     4921 taccatatct gcaattacct catgaaatga atccattctt aggatatcgt gcattgcgta
     4981 tcagtttatc tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca ttgcttcgtg
     5041 catctgttca tggtaattta cgtatcatgt tccctatggt agctacactc aaagaattcc
     5101 gcgcagcaaa agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa ggcaaggaag
     5161 tatctgatac gatccaagtc ggcatcatga tcgagattcc agcagcagca gtcttagcag
     5221 acaagtttgc aaaagaagta gatttcttct ctgtaggtac gaacgatttg attcaataca
     5281 caatggcagc agaccgtatg aacgaacgcg tttcttactt gtaccaacca tataacccat
     5341 caattttacg tttgatcaaa aatgtaattg atgcagcaca cgctgaagga aaatgggcag
     5401 gtatgtgtgg agaaatggct ggagatcaaa cagctgttcc attgctagta ggtatgggtc
     5461 tagatgagtt ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta atgaaacgct
     5521 tggacacagc gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc gatatgatgg
     5581 aagaagtcgt tgaacttgtt catgaatatg taaaataaac aacgtatcaa aagctggtgt
     5641 ggcttgtaaa agagctacgc cagttttttt gttctttttt aagaaaacgt ttttgtcagt
     5701 aagaatagac aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat
     5761 ataagtgaag ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt
     5821 gcaaaatctg gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt
     5881 atctcttata cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga
     5941 atcaatagtc ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat
     6001 gcgcattcta cagaggaaga tttccgaaat tcctttatca gatcgaacca gctttctcct
     6061 atcgtaaaaa aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca
     6121 ccttattcaa aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc
     6181 aatgggatcg acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa
     6241 tatttcaaac tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt
     6301 aaaggaatca ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg
     6361 tttggtcatg ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat
     6421 catcctgaaa atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat
     6481 tcaggtgcag atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta
     6541 gaagctttag caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg
     6601 ttagaagata gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc
     6661 caacagatta ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa
     6721 gagcgaagta tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat
     6781 gaaaaagtct ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa
     6841 aaacatgaaa atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac
     6901 atcgatcaaa acattaaaac aagaattaga aagacatggg catgaagtct atatttttac
     6961 aacgaccgat cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc
     7021 atttatttct ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt
     7081 tgccaaagaa ctagaactag atcttattca tacccataca gaattcggag cagggatact
     7141 tgggaagatg gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta
     7201 tgaagactac cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata
     7261 cttgtctaga ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat
     7321 tgatacatta cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga
     7381 cattgaaaaa ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc
     7441 actaggtctt caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa
     7501 aaatatccaa gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag
     7561 attagttatc gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact
     7621 gcagttagaa gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta
     7681 ttataaagcg gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta
     7741 tacagaagcg atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg
     7801 attgttcgat catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc
     7861 acttattgat tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact
     7921 ttatgatatt tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt
     7981 tgtttatcat gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa
     8041 aataaaatta aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt
     8101 ttctagcgta tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga
     8161 aacttggttt tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag
     8221 caggacacga agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag
     8281 aaggagctat ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta
     8341 caatggtagg ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt
     8401 ctgcagatat ctcaggacat attttagttg atttgactac aagtacacca agcctggcag
     8461 aaaaaattgc aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg
     8521 gtggtgactt aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg
     8581 tttatgatca agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt
     8641 ctgctgggaa aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga
     8701 caggaatgac ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga
     8761 ttgagacgct ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta
     8821 ttttaaaaga agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga
     8881 ttgcattaga agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga
     8941 aactatatga agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca
     9001 aactttggtg gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta
     9061 gagaaaatca atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga
     9121 ggacctccga tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc
     9181 gagtcagcag ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt
     9241 acagtaactt atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag
     9301 caatacgaat ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa
     9361 attttaggat aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa
     9421 tttagttgct tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct
     9481 aattttttgc ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga
     9541 cgtttcaaag ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca
     9601 gaggtaatca ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc
     9661 tcatctgctg ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt
     9721 tcctggatcc gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct
     9781 ttttcgaaat tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc
     9841 tttttatttc ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt
     9901 tcctgtaggt caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc
     9961 atttgataag tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa
    10021 ggcccgtatg ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt
    10081 acataattat aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg
    10141 atcaattgac actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa
    10201 tctgttatat gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa
    10261 tagctgctta ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct
    10321 cggtctttca taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac
    10381 ataggatctc cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca
    10441 agttttaggg aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga
    10501 gatagatgat ttttctaata aaagaaatta taaatgctga tttatcagtt gttttaaatg
    10561 agtaaaatct taaa
//
LOCUS       DAZUKA010000096        10369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000096 DAZUKA010000000
VERSION     DAZUKA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.099"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..579)
                     /locus_tag="ACG8PN_002005"
     CDS             complement(94..579)
                     /locus_tag="ACG8PN_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4862142.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    669..1014
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1059..1700
                     /locus_tag="ACG8PN_002006"
     CDS             1059..1700
                     /locus_tag="ACG8PN_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4862143.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1719..2354
                     /locus_tag="ACG8PN_002007"
     CDS             1719..2354
                     /locus_tag="ACG8PN_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4862144.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2372..3187
                     /locus_tag="ACG8PN_002008"
     CDS             2372..3187
                     /locus_tag="ACG8PN_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4862145.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3506..4942
                     /locus_tag="ACG8PN_002009"
     CDS             3506..4942
                     /locus_tag="ACG8PN_002009"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4862146.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5131..7563
                     /locus_tag="ACG8PN_002010"
     CDS             5131..7563
                     /locus_tag="ACG8PN_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4862147.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7793..8860
                     /gene="rlmN"
                     /locus_tag="ACG8PN_002011"
     CDS             7793..8860
                     /gene="rlmN"
                     /locus_tag="ACG8PN_002011"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4862148.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8850..9056
                     /locus_tag="ACG8PN_002012"
     CDS             8850..9056
                     /locus_tag="ACG8PN_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862149.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9053..10156)
                     /locus_tag="ACG8PN_002013"
     CDS             complement(9053..10156)
                     /locus_tag="ACG8PN_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4862150.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3422 a         1780 c         2260 g         2907 t
ORIGIN      
        1 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
       61 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
      121 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
      181 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
      241 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
      301 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
      361 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
      421 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
      481 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
      541 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
      601 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
      661 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
      721 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
      781 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
      841 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
      901 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
      961 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
     1021 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
     1081 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
     1141 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
     1201 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
     1261 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
     1321 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
     1381 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
     1441 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
     1501 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
     1561 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
     1621 caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa
     1681 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
     1741 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
     1801 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
     1861 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
     1921 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
     1981 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
     2041 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
     2101 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag
     2161 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
     2221 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
     2281 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
     2341 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
     2401 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
     2461 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
     2521 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
     2581 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
     2641 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
     2701 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
     2761 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
     2821 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
     2881 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
     2941 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
     3001 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
     3061 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
     3121 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
     3181 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
     3241 agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg
     3301 taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa
     3361 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct
     3421 ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg
     3481 aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga
     3541 ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg
     3601 gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac
     3661 gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa
     3721 agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg
     3781 acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat
     3841 tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca
     3901 agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga
     3961 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
     4021 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
     4081 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
     4141 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
     4201 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
     4261 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
     4321 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
     4381 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
     4441 cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa agttcatcag aagaaactgg
     4501 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
     4561 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
     4621 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
     4681 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
     4741 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
     4801 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
     4861 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
     4921 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
     4981 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
     5041 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
     5101 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
     5161 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
     5221 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
     5281 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
     5341 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
     5401 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
     5461 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
     5521 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     5581 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     5641 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     5701 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     5761 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     5821 gaagcaaggc gcttagctgc aaaagaagct gcagaatatg agaagaaaaa agcggtgagc
     5881 cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag
     5941 ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa
     6001 ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca
     6061 gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat
     6121 gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat
     6181 aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat
     6241 gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct
     6301 gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg
     6361 gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta
     6421 ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac
     6481 ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct
     6541 gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta
     6601 gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg
     6661 atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg
     6721 acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa
     6781 atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat
     6841 gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc
     6901 gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat
     6961 gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact
     7021 caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg
     7081 gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa
     7141 aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt
     7201 gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat
     7261 cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt
     7321 gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg
     7381 caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca
     7441 cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct
     7501 cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg
     7561 taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg
     7621 ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt
     7681 tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa
     7741 atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa
     7801 acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga
     7861 gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt
     7921 cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat
     7981 caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt
     8041 tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc
     8101 agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct
     8161 gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca
     8221 acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat
     8281 cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc
     8341 caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa
     8401 aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc
     8461 gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt
     8521 gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat
     8581 catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa
     8641 agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga
     8701 tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa
     8761 cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca
     8821 attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt
     8881 caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa
     8941 aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa
     9001 gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga
     9061 ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg
     9121 cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca
     9181 tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg
     9241 atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct
     9301 gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct
     9361 ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt
     9421 tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt
     9481 caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca
     9541 agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat
     9601 catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg
     9661 cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag
     9721 gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata
     9781 ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta
     9841 gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga
     9901 cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat
     9961 tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt
    10021 tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt
    10081 ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga
    10141 aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa
    10201 gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt
    10261 gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag
    10321 tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtat
//
LOCUS       DAZUKA010000097        10327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000097 DAZUKA010000000
VERSION     DAZUKA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.100"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..1147
                     /locus_tag="ACG8PN_002014"
     CDS             104..1147
                     /locus_tag="ACG8PN_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4862151.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1320..1544
                     /locus_tag="ACG8PN_002015"
     CDS             1320..1544
                     /locus_tag="ACG8PN_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862152.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1547..1804
                     /locus_tag="ACG8PN_002016"
     CDS             1547..1804
                     /locus_tag="ACG8PN_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862153.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1906..2259
                     /locus_tag="ACG8PN_002017"
     CDS             1906..2259
                     /locus_tag="ACG8PN_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862154.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2312..3106
                     /locus_tag="ACG8PN_002018"
     CDS             2312..3106
                     /locus_tag="ACG8PN_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862155.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3135..4253
                     /locus_tag="ACG8PN_002019"
     CDS             3135..4253
                     /locus_tag="ACG8PN_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862156.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4286..4606
                     /locus_tag="ACG8PN_002020"
     CDS             4286..4606
                     /locus_tag="ACG8PN_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862157.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4861..5550
                     /locus_tag="ACG8PN_002021"
     CDS             4861..5550
                     /locus_tag="ACG8PN_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFS4862158.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5551..5754
                     /locus_tag="ACG8PN_002022"
     CDS             5551..5754
                     /locus_tag="ACG8PN_002022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862159.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            5774..7402
                     /locus_tag="ACG8PN_002023"
     CDS             5774..7402
                     /locus_tag="ACG8PN_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862160.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7453..7824
                     /locus_tag="ACG8PN_002024"
     CDS             7453..7824
                     /locus_tag="ACG8PN_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862161.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7909..8109
                     /locus_tag="ACG8PN_002025"
     CDS             7909..8109
                     /locus_tag="ACG8PN_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862162.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8243..9469
                     /locus_tag="ACG8PN_002026"
     CDS             8243..9469
                     /locus_tag="ACG8PN_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862163.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10066..>10327
                     /locus_tag="ACG8PN_002027"
     CDS             10066..>10327
                     /locus_tag="ACG8PN_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862164.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPP"
BASE COUNT         4019 a         1261 c         2063 g         2984 t
ORIGIN      
        1 caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta cgaataattt
       61 tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa aatttttagt
      121 aggaattcta ttcacaatgg ggctagtatt attatcacct tcagtttcag cagaaagctc
      181 tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca ctagaagcgt
      241 tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg catggcaagc
      301 tccaacaagt ggtactcctg tttatcgttt gtataatcgg tataacggtg aacattttta
      361 cacagttgat gccggagaaa gagataagat ttcacacaca ggatggacgt acgaaggtgt
      421 tgcgttctat tcctacaacg gagcaaatag agttcctgta acacgtttat ataacaaata
      481 cgtgaattgg catcactaca cgttagatca aaatgaaaag aacgtaatta gtaaacaagg
      541 ttggaagatt gaaggtacag gctggtatgc aatgccaaac agcgtaccaa atcctacacc
      601 tgctccaagc actgaaaaag tgttgaacgt tcctgttgta tggcaaggaa atacaatgtt
      661 atgcgaagga gcttctttat tagaaggttt acactacaaa ggtgtagcca caaatcaaag
      721 cttaaccagt tttgtaaact atatgccaaa agctaatgac aataatcctt atcatggtta
      781 ctcaggagag tggcgtcata acgttaatgg aacgtatcaa ggcatgatgg ctgacccagt
      841 agttaaatgg gcaaaaaacg ttggcggtaa tgccgtcaat atcactggtt caggaacgaa
      901 tggaatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
      961 tgctacgcca gagttcaaac aaatgccttg gggtcgtgca gtatggaatg gacatgtagt
     1021 tctcgtggat ggcttcagaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     1081 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     1141 acgttaattg atagaattat caactaaatg aaataagagc ttaattactg aaatggtaaa
     1201 taggctctta tttttttaga ggaaattttt gtaatacgaa tctataacaa aaaatattga
     1261 atgtagtaca caaaagtggt atattaaaat cgtagagatt atgcgaagag gtgattttga
     1321 tgaaccaaag aagaagaaaa aggttagaag aaataagcga gcgactttcc gagctaaagg
     1381 atgagttaga agtaattcaa gaagaagagt acaaagaaaa tatacctaaa aatctacagg
     1441 gatcagaaag atatgaaaaa gcagaagaat ccgttgatca acttgaagaa gctgttgatg
     1501 gtttatccga catattagaa ttgattgaag atatagctag ctagagatga ttaatgaaga
     1561 tttcaggagt gagaataaaa tgattgatat ttcactgata gagaagttac ttttctcaaa
     1621 taagtacacc gcaaaagagc tggctacaag atggcaaata tccgaatcag ctattaaagc
     1681 ctatcgtcaa aataagggaa gcgtaggatg gcgtgattgg aaagggatca gcattggaaa
     1741 agcagaagac ataatgaaac gtgaaggact tcgatcctat accacttctg aaaaagacaa
     1801 ataacttaat gtttgaaaag ataaaatatc tgtcttaaag ctatgtcaaa ttcaaatggt
     1861 gaatgtttgc taaatataat gcaaaaaaag gaagggaaat attaaatgat aagcgaaaat
     1921 ttgtcttatg agaataataa acatctagtt aaagaaaata agaaagtaga agattataag
     1981 aacatgtata tagaaatgaa aaaatcacca ctgtgtattc aatataaaaa tgataattgg
     2041 aagcaagtag tcgaatttgt aaaacctcat agtatacgaa gagtacgaag ggggaagaaa
     2101 aaaagtgaac ttgtaaatag cggaagagcg gtaattagag ttataaccaa agattatcca
     2161 gttgaatatg gagattatat tttaaggtta agttatcatg cttttgttgt gttgaaagaa
     2221 gagcatttca acgagttttt caatttaaaa agtaagtaat catatttttt tgtagacgtg
     2281 tataaagtta actaaattgc gaggtgatga catggtagca aaaaagaata ctctaaaaga
     2341 agcttctgaa aaatatcaag atatgaaaag acgaggatta ataccgaaag acaaaaaagg
     2401 tatatggaga gaatttgtta aggaagtcag tagtgatcga agtggcggtg actttgttgg
     2461 acgatataca gcaaaaggct atagcaaacg attaaggatc actgttgaat atggaacgaa
     2521 tgaatcagct acagagctga agcaaaggta cttagataca cgaagggaag cttcaaaaaa
     2581 atataatgat ttcaagagca gaggtctgat tcctaaaaag aaaaagggaa tttggcgtga
     2641 gtttttcaag gaagaagata gcaatcggaa taagggagac ttcattggac gatacacagc
     2701 aaaaggctac actaagaatt tagggatcaa ggttgaatac ggaactggtg aaagttcatg
     2761 ggaattgaga aatcgctatt taaatacatt gttagagaaa tataaagcag aattattgga
     2821 gttgttcgat gttaatgatt ttatcgagat ggctgaaaag tatggtcatg aagaaggttt
     2881 tatgatcaat gacattatgg ttattgataa ttttacggat ttattagcac ttgcgaaaaa
     2941 agcaagggat ttaagagaaa cgcagatgta tctattcaga aataaaaacg gaagaaaccg
     3001 gagaactgaa tcgagtgaga ttgaagatta ctacgatgcc tttacacacg atctgggagc
     3061 ctttgtagac gagataagtg gtgtaatgaa caaatatagg gtctagaata aaaaagccgg
     3121 agatgagcag ataaatggga aacaatagca tatacacacc tgaaatgcag aataaaatta
     3181 aaagacttgt cggattaggg tggatgacaa gaggaaaatt agattctttt agtacgatca
     3241 aatatctttt gatggacatt taccgtcaga acaagcaaac aggaatacat tttttgggct
     3301 atgataagcc ggaagatgca cctttctttg ttccaagttc agcctataga gccagtacct
     3361 ccggtagcat gtataacttg gatttgtttg aggaaggagc aaagatgatc gaatatgatt
     3421 tcaacgaagc ctattcgagg atcatgagaa attatccttt gccgtcaaac acttatttga
     3481 aagcaaaata taatacagat caagctattt tgagagcgat aaatagacca aaagtaagac
     3541 cgtatgaaaa tttaagaacg tatatgttct ttttggttaa ttttgaagga acagccaaag
     3601 aaggaacata tgctaatcca aaatcacagt cgatgtttgc ggattatggc gaaaatccca
     3661 aaacagctaa tgagcctatt tggttatcgg agattgagct aacacttatc cttgattatt
     3721 atgatattaa agagatggaa gtcctagata gccacacatt tttgacgaaa aaaggattac
     3781 tcaaagaata ttttgaaaga atcgagccat taaaaactga tcctgatacg ttgaagcttt
     3841 ataaaaatct tagaacgaaa gtatttggtg ctatcggaca aaaatcatta agagaaggag
     3901 aaacaagcac cttcgataaa tacagtatgt ataatcgagc gtattcctcc atggtagcag
     3961 gcgtatttag agatcgtatg gttagatatg aacagaaata cgttcatagc gcatatgggc
     4021 tattaaaaat tcggactgat ggattgtatt tcagatccga agtaccagag tttgaagagc
     4081 tgtataaact tggagtagtt aagaaaaaag ttcatacaat atccaaagaa gaagtcgaaa
     4141 aagctttaga atttaaaaac aaaggatttg aaaataaggg ctcctttggg aaatctaaag
     4201 atgaagttaa gggtgttgtg atagacgatg atttcgagtt tgattttgac taaatactct
     4261 ttggaaaaga tggtaggagg taaatatgaa gaagaaaatt gtggaaggaa atgtaggtgt
     4321 tattattcta aaagattcaa ctgactatgc taaatcaatg gcactaaaag acgaaaaagt
     4381 tgcacgcatg gataaagatt tagttgaatt tctgatggat gatacagaaa gattaaaagg
     4441 ggatcagttt ttgagtttaa tgaaagacaa aggcttaaac acatatggtt tatttgatta
     4501 taagaaatta gctatagagt gggtaaaaga aggagaaata ttttttattg aagaatggga
     4561 aggtactgag aacttagttc ttcatcagta tcttttgaaa gcttaatcta agtaaaacag
     4621 ttctgactag ttatgcttgt cagaatttca aaggaagttt tccataacca tcatcattca
     4681 aaaataaagg atttgaaaat aagggttcct ttgggaaatc taaagatgaa gttaagggtg
     4741 ttgtgataga cgatgatttc aagtttgatt ttgactagat ttttttgaaa ggtttaagag
     4801 taaacaaggt aagaactaag atcatgaaga ttgagagtaa agaagataga ggacgaatgt
     4861 atgagaacac actatattta tttagaagat aaaattgtag atgaaattcc ggcaaaaagt
     4921 cgagaacaag ctagaaaatt atttattgaa aaatacaaag agatcaatgg agatagtgaa
     4981 gttccaaaag tggtcgtaac agttagaaaa aggcaaacaa aaaagagaag tacgtttcat
     5041 gtcagccctg atatatataa tcttatagag cgacatagaa gtgaagataa tatttctatc
     5101 tctcaatata ttgctacagt tattgatgaa ttgatttcag gaaagtatga ctatgctttt
     5161 gattttgagg gtgaaaagga ttacaaaagt gttgctgtct ttcaatctga ttttgagaag
     5221 ctaaaaaact atgctggcag gagaaatgtt aattttgcaa gactactaga agagttctac
     5281 tccgaagagt tgggagtagg aaatgaatac aagagagaaa cttatgcgaa attgtctcat
     5341 aagttatccc aagcgcttaa agaagtgaca gaagttaaag cggaactaga tgctaaactt
     5401 gaagaagtga aaaaatatca aacagagagg aagaacaaca tgaagaatca agtggaattt
     5461 gacgaacaga aagaatttga aaaagcaaga aaacgctata acaaaattat gaatgaactt
     5521 agctatgaac acttaacagt tcatgaataa ttgagtgagg gtacagaaaa atatacgatc
     5581 gcagatatgg ttaatgaagt ggaaactctt ttagcttctt tctttgaaaa tggtcacgta
     5641 aacgatgaat ggcgaaaaga agaacctaaa agggttaacg cttatattgc tcgattaaaa
     5701 aggtttattg ataaatataa ggagaatagt gacaaatttt ctaaagataa ataaggagag
     5761 agtgtgatta catttgccag aaatcgaagg aatattttcg ccaaaaatag gtaaaaaaca
     5821 gttagatttc agcagttttc cgagcgtaaa tagagattat aatctaggtg atgaggtaca
     5881 gatactaaat attgatttca aagaactaga cttcgctgtt gctgtttcca acaaacccaa
     5941 ggcattggaa cgagtgaaaa aaatatacca agagaatgat caaaatatca aaaatatgat
     6001 caaaaagtat agacgagata attcaaaagt tgaagacgtt ttattcaacg gtacggcaaa
     6061 acatgatttg aatgtttata tggcattagg agtggctttg tactgtaatt caattaatca
     6121 gacagatatt ttggttaata tcgtgaggaa tagctatcaa aaaatattaa aaataagtag
     6181 cgaaacaaac ccttattatg caatgaaata tcttggggat aatttattaa atgataaaaa
     6241 attgatgcac aacactgaag agctgttatc taatttggct gcgattaact ttctattcaa
     6301 aacaggttct gaagattatt ttatagataa cgtactaaaa ggtgcttacg gaacagcatt
     6361 ttatccgctt tacaatatga aacagcataa agccgaacaa aaggataaca aacttgaaat
     6421 taaaaagatg aaagaactat taggtatagg atttgtaaaa cccaattcaa aagtcactat
     6481 tgcttcgtta gcaactacta acaatgatgg gaaaatagta aatgggtttg acgattcaga
     6541 tatagatata ttgtcaaaat catatttttc tttgtatgga gattttgcag atttgatggg
     6601 tattgatagt tacttagtca ataattttga gatcaagggt agcgagctga atgatattgt
     6661 gtatttcgtt ttaatctctc tatatgcaat tgatcagaat accgagtacc gacaaattga
     6721 ctatttgcta gttttttata gtttgttata tcagttcgtt ttaactcggt tgtttaaaaa
     6781 tgctaaggaa gaattgatcc tgaattctga ttatgaaata gcaaaacaaa ttgcaagtat
     6841 tagaaaagaa aacaaatctg aagtaatcaa attaagaaaa gaattaagta ttgcgagtaa
     6901 agaaaacggt cggttagaat ctttagtcaa atcggcaaaa aaagaagagg aacttttgaa
     6961 gcaagagatt aaagaattaa aaggaatcag aaaagaactt gaacagctga ggtttgaaaa
     7021 taaagtatta aagcaattgc aggagcaaga acatagacaa tcattaaatg gtaaaactga
     7081 agagccttca ctgtctgaaa tgatagctta tctaaaacat aaaaacattc ttttagtggg
     7141 aggacatgta aattttcaaa ataaattaac tgaactatta ccaaatatta aaatgtttga
     7201 tgtaagcgaa aataatggaa agatacttaa aaatttgaat acgtgtgatg cgatttttct
     7261 atatacagat tacttgaatc atggtctata ttatcgtgtt atgagtgaaa tttctaataa
     7321 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
     7381 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
     7441 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
     7501 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
     7561 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
     7621 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
     7681 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
     7741 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
     7801 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
     7861 cctattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
     7921 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
     7981 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
     8041 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
     8101 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
     8161 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
     8221 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
     8281 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     8341 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     8401 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     8461 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     8521 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     8581 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     8641 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     8701 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     8761 aagaacgcct agaatatata aaaatagaaa caaatatgga ttattacctt ttagaggtcg
     8821 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     8881 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     8941 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     9001 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     9061 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     9121 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     9181 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     9241 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     9301 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     9361 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     9421 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     9481 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     9541 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     9601 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     9661 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     9721 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     9781 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaaaatgat
     9841 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     9901 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     9961 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
    10021 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
    10081 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
    10141 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
    10201 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
    10261 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
    10321 ccaccac
//
LOCUS       DAZUKA010000098        10301 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000098 DAZUKA010000000
VERSION     DAZUKA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.101"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..353
                     /locus_tag="ACG8PN_002028"
     CDS             204..353
                     /locus_tag="ACG8PN_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4862165.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            364..1878
                     /gene="dltA"
                     /locus_tag="ACG8PN_002029"
     CDS             364..1878
                     /gene="dltA"
                     /locus_tag="ACG8PN_002029"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4862166.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1875..3080
                     /gene="dltB"
                     /locus_tag="ACG8PN_002030"
     CDS             1875..3080
                     /gene="dltB"
                     /locus_tag="ACG8PN_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4862167.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3125..3358
                     /gene="dltC"
                     /locus_tag="ACG8PN_002031"
     CDS             3125..3358
                     /gene="dltC"
                     /locus_tag="ACG8PN_002031"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4862168.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3361..4629
                     /gene="dltD"
                     /locus_tag="ACG8PN_002032"
     CDS             3361..4629
                     /gene="dltD"
                     /locus_tag="ACG8PN_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4862169.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4770..5852
                     /locus_tag="ACG8PN_002033"
     CDS             4770..5852
                     /locus_tag="ACG8PN_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4862170.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5852..6655
                     /locus_tag="ACG8PN_002034"
     CDS             5852..6655
                     /locus_tag="ACG8PN_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4862171.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6725..7465
                     /locus_tag="ACG8PN_002035"
     CDS             6725..7465
                     /locus_tag="ACG8PN_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4862172.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7476..8480
                     /locus_tag="ACG8PN_002036"
     CDS             7476..8480
                     /locus_tag="ACG8PN_002036"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4862173.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8525..10300)
                     /locus_tag="ACG8PN_002037"
     CDS             complement(8525..10300)
                     /locus_tag="ACG8PN_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862174.1"
                     /translation="MILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDF
                     KFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDY
                     QEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQS
                     VPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGI
                     LFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFT
                     PAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQT
                     ALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQ
                     LRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQV
                     TNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPL
                     YRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTG
                     TLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3307 a         1702 c         2084 g         3208 t
ORIGIN      
        1 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
       61 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
      121 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
      181 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
      241 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
      301 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
      361 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
      421 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
      481 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
      541 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
      601 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
      661 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
      721 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
      781 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
      841 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
      901 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
      961 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
     1021 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
     1081 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
     1141 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
     1201 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
     1261 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
     1321 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
     1381 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
     1441 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
     1501 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
     1561 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
     1621 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
     1681 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
     1741 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
     1801 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
     1861 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
     1921 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
     1981 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
     2041 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
     2101 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
     2161 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
     2221 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
     2281 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
     2341 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
     2401 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
     2461 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
     2521 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
     2581 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
     2641 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
     2701 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
     2761 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
     2821 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
     2881 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
     2941 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
     3001 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
     3061 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
     3121 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
     3181 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
     3241 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
     3301 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
     3361 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
     3421 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
     3481 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
     3541 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
     3601 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
     3661 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
     3721 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
     3781 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
     3841 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
     3901 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
     3961 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
     4021 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
     4081 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
     4141 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
     4201 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
     4261 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
     4321 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
     4381 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
     4441 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
     4501 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
     4561 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
     4621 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
     4681 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
     4741 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
     4801 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
     4861 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
     4921 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
     4981 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
     5041 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
     5101 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
     5161 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
     5221 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
     5281 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
     5341 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
     5401 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
     5461 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
     5521 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
     5581 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
     5641 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
     5701 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
     5761 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
     5821 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
     5881 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
     5941 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
     6001 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
     6061 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
     6121 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
     6181 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
     6241 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
     6301 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
     6361 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
     6421 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
     6481 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
     6541 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
     6601 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
     6661 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
     6721 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
     6781 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
     6841 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
     6901 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
     6961 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
     7021 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
     7081 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
     7141 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
     7201 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
     7261 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
     7321 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
     7381 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
     7441 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
     7501 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
     7561 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
     7621 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
     7681 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
     7741 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
     7801 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
     7861 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
     7921 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
     7981 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
     8041 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
     8101 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
     8161 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
     8221 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
     8281 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
     8341 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
     8401 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
     8461 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
     8521 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
     8581 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
     8641 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
     8701 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
     8761 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
     8821 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
     8881 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
     8941 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
     9001 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
     9061 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
     9121 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
     9181 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
     9241 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
     9301 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
     9361 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
     9421 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
     9481 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
     9541 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
     9601 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
     9661 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
     9721 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
     9781 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
     9841 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
     9901 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
     9961 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    10021 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    10081 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    10141 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    10201 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    10261 aataagcaat tcctgttaaa aataacaatg ataatataat g
//
LOCUS       DAZUKA010000099        10300 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000099 DAZUKA010000000
VERSION     DAZUKA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.102"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1118)
                     /locus_tag="ACG8PN_002038"
     CDS             complement(270..1118)
                     /locus_tag="ACG8PN_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4862175.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1282..2184
                     /gene="gnd"
                     /locus_tag="ACG8PN_002039"
     CDS             1282..2184
                     /gene="gnd"
                     /locus_tag="ACG8PN_002039"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4862176.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2186..3187
                     /locus_tag="ACG8PN_002040"
     CDS             2186..3187
                     /locus_tag="ACG8PN_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4862177.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3184..3468
                     /locus_tag="ACG8PN_002041"
     CDS             3184..3468
                     /locus_tag="ACG8PN_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4862178.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3501..4298
                     /locus_tag="ACG8PN_002042"
     CDS             3501..4298
                     /locus_tag="ACG8PN_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4862179.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4312..5145
                     /locus_tag="ACG8PN_002043"
     CDS             4312..5145
                     /locus_tag="ACG8PN_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4862180.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5163..6020
                     /locus_tag="ACG8PN_002044"
     CDS             5163..6020
                     /locus_tag="ACG8PN_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4862181.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6042..6551
                     /locus_tag="ACG8PN_002045"
     CDS             6042..6551
                     /locus_tag="ACG8PN_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4862182.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6588..6989
                     /locus_tag="ACG8PN_002046"
     CDS             6588..6989
                     /locus_tag="ACG8PN_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862183.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7101..7454
                     /locus_tag="ACG8PN_002047"
     CDS             7101..7454
                     /locus_tag="ACG8PN_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862184.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7488..7931
                     /locus_tag="ACG8PN_002048"
     CDS             7488..7931
                     /locus_tag="ACG8PN_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4862185.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7948..9033
                     /gene="uxuA"
                     /locus_tag="ACG8PN_002049"
     CDS             7948..9033
                     /gene="uxuA"
                     /locus_tag="ACG8PN_002049"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4862186.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9026..9739
                     /locus_tag="ACG8PN_002050"
     CDS             9026..9739
                     /locus_tag="ACG8PN_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4862187.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9946..10095)
                     /locus_tag="ACG8PN_002051"
     CDS             complement(9946..10095)
                     /locus_tag="ACG8PN_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862188.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3440 a         1631 c         2238 g         2991 t
ORIGIN      
        1 tttttttaat tttttagaaa ataagaaaca aaaataataa aaaaaagaaa agataaattt
       61 tttactactg tatactaact ttgttatcgc atctaacaag taactcgaat aataccaata
      121 caaccttctc caaagatgcg atgacatacg ttctaaccca ccgactattg atgttctgta
      181 ccacccttgc agaaccaaaa aagccactac caaatatttg gtagtggctt tttttgtttt
      241 atggattttt cgctttttct gttatggtgc tattctttga tttcagaaat agtatgtcga
      301 actttagcga tagaacgacg tgattctttt tcgttgtaaa acatcaaaat cacatataga
      361 gagtccaaaa tgcttagttg agaaatacgg gaagctaaag cttcagatcg gaattctgtt
      421 tcttcagaaa tagaaataaa aacgacatcg ccaagtttag caagggaaga attagcttgg
      481 ctagtaataa cgattgtttt tgctccagtt tttcttacgg tttcagcaat atggattgtt
      541 tcttttgatt gccctgtgtg agaaatcaaa acagcacagt ctttttccgt caataaagaa
      601 gcttccatca attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa
      661 aatttatgat aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa aaaatagaca
      721 cgtttagatt cattgataat agtagccgct ttttttagat ccttttgttg taacaacttt
      781 tttgtgtcat ttaaagttga aatactagac tcgaacacct tttgcgccat agtgagctca
      841 gtatctgttt tctgaatatt ttcgtggata gaaatagcag atagatcatt ttcttggatc
      901 aacatggcca ttttaaaatc tttaaatcca ttataaccaa gtttttttgt aaattgaaaa
      961 aaagtagagt cagcaatgcc cagttcactt gctaaattgc taataggact atgacttact
     1021 ttgcttggat tttctaaaat ataatcggcg atagcctgtt cttttgggct gaagtcgttg
     1081 tacattgttt tgatttgtag tcttatggag acgctcatga gaattcctca ttttcataat
     1141 aagattatca ttttttccgt tctcattaat gttaaagata caaggagagt aggaaaaaat
     1201 caaacaaaaa atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt
     1261 ataagaaatg gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg
     1321 aatttagctt taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt
     1381 acagctgtta gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt
     1441 ctagctactt ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc
     1501 gaaaacatgt taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc
     1561 ggaaactctt attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc
     1621 ggttacttgg attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta
     1681 atgattggag gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca
     1741 attgaaagag gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc
     1801 cataatggaa tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa
     1861 aaaagtgagt atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg
     1921 attcgcggct ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt
     1981 aattatcggg gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta
     2041 gaaatggctg ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag
     2101 gaagaagata gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat
     2161 gaaatagttg aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag
     2221 cagtttcgga agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca
     2281 tttctttact gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt
     2341 caatatcatt attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga
     2401 tgaactgcta ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc
     2461 aaaagaacac cagacaatcg tatctatcat acctgcttta attgaacgaa atgctgtagc
     2521 agaaaataat ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag
     2581 agtcaaggac gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa
     2641 aacagttggt gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa
     2701 cggttttcgc tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag
     2761 ctttggtgca aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt
     2821 atgtgcgaat ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat
     2881 gaaagaaaat gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat
     2941 gattaatggt ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga
     3001 accgggacgt caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat
     3061 atcggcctac acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga
     3121 acagatcagt cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa
     3181 aggatgatga agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta
     3241 tatgaagaag gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga
     3301 gtactggtca cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg
     3361 aatgacgaaa cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa
     3421 gccagacgaa tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg
     3481 gaggaaaact gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag
     3541 tcatccctac tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg
     3601 agaatggaca agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa
     3661 cagatcccca attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata
     3721 cactagcaaa atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat
     3781 catttttgaa gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt
     3841 ctgctaaagc aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt
     3901 cagagattat ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa
     3961 atgcggcagc acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt
     4021 ttctacatgg agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct
     4081 gggaaaaaat agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag
     4141 aacaaatggg attatttccg aaagataaac agataataga taaaatggct gaatttattg
     4201 attcgaaaga gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt
     4261 caatgatgga actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca
     4321 ttaagtgtag tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg
     4381 ttaggtggag gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc
     4441 gggttgatca tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt
     4501 tatatggggg ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct
     4561 atcgcagtac cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg
     4621 gtgccagtcg gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt
     4681 ttaggtaaat atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca
     4741 attattcttt atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct
     4801 ttatatttag gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt
     4861 atccacattc ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta
     4921 tacgttatcg gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt
     4981 gttttgaaat ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct
     5041 ttgatctttg tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat
     5101 tcagcatctt ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg
     5161 aaatggctga aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg
     5221 taacaaaagc ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt
     5281 acttggcgcc atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg
     5341 aagactcttt aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct
     5401 ggggtggcgg tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag
     5461 agtatgaaga aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat
     5521 tgatgggggc actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt
     5581 ttatcgcaat cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt
     5641 ttatctgctt tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca
     5701 ctatgggccg taatgctaca ggcctaatgc aaaacactgg tgttcagaca gcgattgaaa
     5761 tgctatcagt tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag
     5821 aatcttcatt gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc
     5881 aaatcgtgcc agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga
     5941 aaaaaggatt gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag
     6001 caggaatcgg tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag
     6061 cgcgtgtgga tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg
     6121 gagtgaattc catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc
     6181 tttacaaaaa tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag
     6241 ctaaattcta ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa
     6301 cagtccaaac gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg
     6361 gagggattgc tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa
     6421 ataaatcaga tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt
     6481 attttcaagc cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg
     6541 atttgtcata aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg
     6601 agctgtttcg tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa
     6661 agagtaaaca gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg
     6721 tgacagtcat tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata
     6781 tcgaaaaagc agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa
     6841 gttatttcat gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt
     6901 ccttcatttt gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc
     6961 atcacgtatt cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct
     7021 actttttctt attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa
     7081 atacttcatc catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag
     7141 cgtatgcgtt tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg
     7201 gtatgaaaaa agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac
     7261 agttttcggg aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga
     7321 aagaaaaaaa cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt
     7381 tgaccaaaac agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg
     7441 ctctttttcg ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca
     7501 tgatcgttac acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg
     7561 ggacatcgag ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg
     7621 gagagaaggt caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg
     7681 ttgatctaat tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg
     7741 aagaaaagaa agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag
     7801 caatcaatca tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac
     7861 aaggaaaaga taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg
     7921 acgattttta gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa
     7981 ggggattcga tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga
     8041 actttgctga ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa
     8101 gcatctgttg agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat
     8161 gcaatcaagg cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc
     8221 aaaaacttgt ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga
     8281 tgggcgaaaa ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat
     8341 cagaaaatcg tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca
     8401 aagggattca aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa
     8461 aaactgtatg aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa
     8521 aaagtgattc cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg
     8581 ccatgggaac tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt
     8641 ctaaatgcag tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca
     8701 aatccggaaa acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc
     8761 cacttcagaa acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct
     8821 acagaaggct cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac
     8881 ggtcctatac gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat
     8941 ggcctgtatg atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt
     9001 aaggaaaaac cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga
     9061 tggcctttta ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa
     9121 gtatgattat gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc
     9181 gataagagaa caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga
     9241 agcaatcaat caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa
     9301 aggagcagaa gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc
     9361 gtctaataca cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt
     9421 tgatcaaatc atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt
     9481 gaaagcttgt tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat
     9541 tgcagggatc gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt
     9601 gatagggttg cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga
     9661 atccaataaa gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag
     9721 taaagggttg tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg
     9781 cacgactgag ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt
     9841 tgtagtgaac aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg
     9901 tttaaagaga agaacgagaa tcgtccatat gagggtttcc actccttatg cagatttttt
     9961 taattcttgc actgcttgtt cgatcgtaac acctgtcgct acatgattgg tattttcttt
    10021 gtcaaaaacg acaaatagat tcaaatctgt atccaaggtt actcgatatt gtatatcttt
    10081 gttatagaat gtcatagcca ttctcctttc tttaaatact tgctgaaagg caacgaacgc
    10141 gttgataacg aaattccatt tataagtata ccaccaattg aaaggttttc atagttaaga
    10201 aattttaaag tcaaaagttg cagataaaag caaataaaca agaaaacaaa aagtgaatca
    10261 atcgcggtta ttcaactttt tcttcgattc gaagacaata
//
LOCUS       DAZUKA010000100        10161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000100 DAZUKA010000000
VERSION     DAZUKA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.103"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..956
                     /locus_tag="ACG8PN_002052"
     CDS             87..956
                     /locus_tag="ACG8PN_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4862189.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            976..1323
                     /locus_tag="ACG8PN_002053"
     CDS             976..1323
                     /locus_tag="ACG8PN_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862190.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1336..2598
                     /locus_tag="ACG8PN_002054"
     CDS             1336..2598
                     /locus_tag="ACG8PN_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862191.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2678..4054
                     /locus_tag="ACG8PN_002055"
     CDS             2678..4054
                     /locus_tag="ACG8PN_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4862192.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            4051..4680
                     /locus_tag="ACG8PN_002056"
     CDS             4051..4680
                     /locus_tag="ACG8PN_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862193.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            4671..5162
                     /locus_tag="ACG8PN_002057"
     CDS             4671..5162
                     /locus_tag="ACG8PN_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862194.1"
                     /translation="MAVEVKIVLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEI
                     KKLYFSKYNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEI
                     IISKYGIAYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEG
                     EKK"
     gene            5159..5641
                     /locus_tag="ACG8PN_002058"
     CDS             5159..5641
                     /locus_tag="ACG8PN_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862195.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            5638..6300
                     /locus_tag="ACG8PN_002059"
     CDS             5638..6300
                     /locus_tag="ACG8PN_002059"
                     /inference="COORDINATES: protein motif:HMM:NF019467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4862196.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            6287..6655
                     /locus_tag="ACG8PN_002060"
     CDS             6287..6655
                     /locus_tag="ACG8PN_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4862197.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            6736..7188
                     /locus_tag="ACG8PN_002061"
     CDS             6736..7188
                     /locus_tag="ACG8PN_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862198.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            7897..8496
                     /locus_tag="ACG8PN_002062"
     CDS             7897..8496
                     /locus_tag="ACG8PN_002062"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4862199.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            8512..8988
                     /locus_tag="ACG8PN_002063"
     CDS             8512..8988
                     /locus_tag="ACG8PN_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862200.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            9005..9907
                     /locus_tag="ACG8PN_002064"
     CDS             9005..9907
                     /locus_tag="ACG8PN_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862201.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            9980..>10161
                     /locus_tag="ACG8PN_002065"
     CDS             9980..>10161
                     /locus_tag="ACG8PN_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862202.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRS"
BASE COUNT         3872 a         1356 c         2068 g         2865 t
ORIGIN      
        1 taaataaaaa tacatttgac aaaatttaca atatagttta ttatatattt atcttattat
       61 gataacacaa taaaaggaat ggtggtatga aaaagaagaa gtttagtcga tcgttgttag
      121 gaattttggt tttagttgcg gttggtctta gtggcaaggg ttactttcag aataacagca
      181 ctcaggaaca tgattttcag atcaaaagta gtgttcagat aggtaataaa gcagacagtg
      241 aatcaaaaga atttttaggg atacgttatg atccaaacaa atatccaaat aatatcgtaa
      301 acattaatga tgggaaatat acactgacaa aagaagacca tgaagcatta tcagaaaaag
      361 gcagtcaaac cttttgggta aattatgcag atttagatca acttggaaga gcaggagatg
      421 ttgaagcgtt gatcacctat gatgctgtaa attctcactc atctaaaaat gtggaacgtc
      481 catctttcaa aagttctgtc catattgcag gagaatacaa ggatggagta tttgatcaag
      541 agagagatac ttgggtcggc aaagattcaa acaatgaaat cactcagcta gaaggctata
      601 aaggttatct ctataacaaa agtcattcat tagcatggtc gctaggagga gatatggagg
      661 tacacaattt aacacttgga acgagagcgc aaaatgttgg gaaaaatgat cagaatggag
      721 ggatggctta ctcagaaacg attgtacgtg atgcaatcta taaaaataag aatgaaaaag
      781 tattgtataa agttgatcca atttataaag ggaatgaatt agttcctcgt ggttctcatg
      841 tcgaagcttt ctcagttaac gataatggaa aaacggtaaa ccttaatgtt tatgtattta
      901 acaatcaaaa aggaatcaac atcaactatc aaaatggtac ttgggtaaga gattagaaat
      961 tgagaaggtg aagaattgga aacaataagc atcaatggat tagataaatc agttgcaaat
     1021 cgaataaaaa aaggaaatgg ctactatctt ctgaagaagg gtaaatattc agttgctaga
     1081 gagttagaaa caatgagtat cgtgtatgta gctaagaatc attttgacgt agaacaggct
     1141 atttttgatc cagattctga aatagggaaa tgtgttgaat tatcagagaa aactgttagt
     1201 acaaaagggg cgatggtcat tgagagagaa atgttagacg aaatcagagt aatcattcct
     1261 aaagatacgc agatgtgtga ggacagcata agtgacgatg aaccaaaatc agaaagaaaa
     1321 taaggagata ttcagatgag cgaaaaatcc attgaattaa aggaatataa gcgaagcgat
     1381 ggagaaaaat attatcctgt agccagtttt tctaaatcag tatatctggt tggacgtgca
     1441 ttggtagttg gtgcaattgg catatatatt ttgcattggg taacaaaggt tgtacaactg
     1501 cttgtagata ccttagtaag tattcttgtc tcgttaaatg atgttaacgg aacagatgat
     1561 ttaggattaa aagagatgtt taaagagtta ctcaatgcgt tttttcccaa ttatttaagt
     1621 tattcatata atgtacaaag cttttttgca aagctacact tggtagacat ctctatttta
     1681 gtattagaag caggaacggt attctttgtt ggatatttgg ttactttgat gttcaggcat
     1741 cactatggag aacaaagacc attttttgat gatatggaaa gtagaaggtt gaaaagagaa
     1801 gctctagaag ctatggatgt gaagtatgat ccaaacaaga ttataggaga tgaaacgaac
     1861 aacaaaaaaa gaaaacgaaa caaaacgcaa ttagaatccg atgtgctatc aacgcttaaa
     1921 aatatcaaaa tttctgttca tacaagacgt gatctaagtg tcaatgaaga tattcgagaa
     1981 tatatcgtaa aaatcccgca accaaaaggg aagaaagtta gagaaacagt tttacgacaa
     2041 attcaagatt tagggtcgat cctgacaaga gcaacaagcg gacgagccaa atttggtgaa
     2101 aaagaagatg aagtttcagg cgattatttc attttccgag catcagtaaa tattgaaaga
     2161 gaacgtaagg aaagagtaag taccaaaaac aaagaaacgg aaagagcaac atcttcaatc
     2221 gcagtttcaa attcacaatt ttcttttcca atcaatctct ttgccgataa ttccaaaaat
     2281 atccaaaacc aaaccgaaaa agctagaaaa tatgccgaaa ctcaaaagca atctttagaa
     2341 ctttatctat cttctgctga cgtacctgca agtctaacag atatgcacgt tgggaataca
     2401 tctattgaat atttgtttac tttatctcgt accagaaaaa ttcaactaag ctcaatgaaa
     2461 gaagaaattg aaacttactt gcatatatca ggtgtaatcg tgcggatcaa cgcagggcgt
     2521 attcaaatta ctttaccatt aggagaaaat gatcgtattc caattgatgt accatcaatg
     2581 atcaatagcg tgttttagaa gacatcacaa atagaaacag aaaatacaaa ctaccagttc
     2641 acaaacagaa aagtaccgag aaaagtgagg aatagaaatg gctatgaaat tattagagaa
     2701 agtcaaaaag agagccgaaa aagctgaatc tattttgcta gataaagaac ctaatataaa
     2761 gcttaatgga aaattcagtg tcaatatgtt gaacacaaag gctaaagacc caacagatgc
     2821 tatttttgga attaagcctg atggcacaat tgctcatttt tgcatcagcg aaatgccaca
     2881 catgttggtt gcaggaacga caggatcagg gaaatcagta ttactgaatg aaattcttgt
     2941 tacagtaatg tgtcactcaa cgcctgatga attaaaaata ggaattgtag acccaaaaag
     3001 ggttgaattt ggaagataca aaaaattgcc gttcatgttg gctgatccaa ttacagatat
     3061 ggatgaagct tatgattttt ttgaatatct tgtgatcttg atgcatgaac gttacaaact
     3121 gatggaaaaa gcagggttac aaaacatcac gctatataac gaatatgccg aaaaaaatgg
     3181 attagataga tttccctatg tgatcttatt agttgatgaa tattcacagt tggttggaac
     3241 acataaagaa gtagaaggtt tgattgttca attaggacaa atggcaaggg cagcaggtat
     3301 tcatgttatc ttagcaacac aatctcctcg atcaacagtt gtcactggta ttatcaaagc
     3361 caattttccg tcacgtgttt gccttatggt agcaagtgat ttagaaagta ggattgtatt
     3421 agatgaaggc ggtggagaaa gtctaagtcc aaagggagat atgcttatta agcttgttga
     3481 tggaagtatg gttagagcgc agggagctta catcagtaac aaagaaattg aaacaatctt
     3541 caatcacctt agaaatacta tgccagaacc agaatatgtt gattaccgag caatcgtagc
     3601 tgaaactcgt ggtgagggcg gtgaagacga ggattttggt ggaattggta aagctcctgt
     3661 aagaaaatct gctaaaagaa atttgaacac acctttagca aatccatcat ctaaagagcc
     3721 atcaattgaa aagaagagtg cgtttgctat taagcttgaa aaaaataaag aagaaaaagc
     3781 taatcaggga gctaagtcac tagaaaaact tcagcaagaa aaattagaaa aactagaaaa
     3841 agcattatca tcatcaactt ctgaaactca atcttcaaag ggtatagata atggacaatc
     3901 aattacaact aaaaacaagc ctaaaaatag taattcaaat ctctcggtaa atgttgttat
     3961 cacaactgat aaatctgaaa aaaaacaagt gaacggaact acttcaccca tcatgcaaaa
     4021 aatgataaag gcaaaaaaac aggtgcagaa atgattatca ttagttcctt tttgagatgg
     4081 ctaacaccat ataaatatac agatgaaata gttgagaagc gagcgaagaa aaaagttaaa
     4141 aaaacgatgt taactcctaa agaaatgcta gatcgtggaa tattgggagt gaaggtttta
     4201 gaagtaaacc aaccgtttga attggataat gcgagagata ggttgcttag agctgaagtt
     4261 gatgtcgcat acttttatca tgataaaagc aagcatcaaa tagctatcaa attcaatcca
     4321 aaatcggcat ggcgatacca agcaagagat gacctaaagc gtaggaagct gaaaccgatg
     4381 ctctatcctg aaggaaaaga ggcatttgat agaacccata ttgttcccat tggatttcat
     4441 ggtacagagt cagataaccg cctattggta gggtggaaca gctcacaaaa tagagttcag
     4501 atcaataaat ttgagaagaa ggtaagaaaa tttaacgatc ggaaaataat actttggttt
     4561 gccaatattc agaagcaacc agatgggaca gcaaagtggg aagcggtcat atttgatgaa
     4621 gatggcaaaa aagtaatgtc tgaagtatgg catgataaag ctaaatttgt ttggcagtag
     4681 aggtgaaaat tgtgttaact aacctaaaac tatatagaga gggatttaac gaaaaagtaa
     4741 gtatcggtga atctattgaa tatttaggaa agaagtacat tattattcgt attcttgaaa
     4801 ttaaaaaact ttattttagt aaatataata atttagcagt tagagttctc gtccaaaagg
     4861 ttggaaatcc aagtgactat agaagtgatt accataacta taaaacagaa tcatctgtaa
     4921 ttaagcgtta taatcaatca aaaaatgatg agagaatatt caaagtagga gaaattatta
     4981 tttctaagta tgggatagca tacgaaatag tttctattaa aaaattttat tatgaatttg
     5041 ttgatttggt tgtagaatat tctgttcgat tgatcgttcc gtggagtaaa gaagaaatta
     5101 atgaggcatt aaaacatgaa cgaaaaagta cattcaaagt tttagaaggt gaaaaaaagt
     5161 gaaaacagac cttactaggc aagctgagaa gtgcttatgg catgaaacaa atcgcatggg
     5221 tgtatttggt tgtttcgaag tcactatcgg ttgggatggc agtgaaagag tagattatat
     5281 aacttattca gtagataaca cagttcgatg ttatgagatc aaagttactt tagctgattt
     5341 aaaaagcaag gcgaaacaga cttttatcgg tgattataac tatttagttg ttactagtga
     5401 tttgtgggag aaaatgcaac aagacgataa acttatgcgg aaatattact atcaaggcgt
     5461 atacgtgttc aatgaaaaat atcctagcat aataagtgtt aagaaagcaa ggaaacaaaa
     5521 agtatcgcta ggtacaagat caactgtatt agaaagcatg gttcgttcta tgaatcgtga
     5581 agttaagaaa ctctataaag taaaaccatt ttgggagata gcagaggtgg aaaaaggatg
     5641 attcaaacat ttgcaaaaat gagtagaagt gcttggattg aaaagctgga aaatgatttg
     5701 agtgttatgc gattagctcc aaagaaattc aaacgagaga ttgcagaaaa agaagcaatt
     5761 ttgtcggtat tgaatgcggt agaactggac gaaccgaaaa agtcagttga tgtacctgaa
     5821 aatgtagcag agttactaga ttattatcgt aattcgtcag atgtcgattt gttagctctg
     5881 atattaacat ttcacgattg gtattactgt caaaatgaag atactcagca tgataaacat
     5941 gataaagcag ttgactggtt aatagagaat ccagaaaagt tcatgcgtgc ttggctagat
     6001 ggctatgata gcaagaaaaa gccactggaa agtaatgagg aaacgtattt aacggcagaa
     6061 caatatgact atgctagaaa aacaggagat tgggaatctg gtaagacttt caatcacaga
     6121 aaagatcaac caaaaattag tttttcagat atagccgcaa ttatcgaagg tatctactat
     6181 ggacaagccg atgaagagat cttttctata caagatttat tagaattttt gaacaaaaat
     6241 aaggatagct ttgtagaact aactgattat gaggaggata cagcaaatgg aaaattatga
     6301 tccaaatatg cgatggggta cacatacgct taaagtttct tttcaacgtt gggattacaa
     6361 gggatttgtg acttttagaa aagctggaaa ttgtaaagga ttagatgtct tagcgttgga
     6421 tgaagactat ctatatgacc atccattgac ggataatcca atcggatttg gattgcttcc
     6481 tgaagatgat gagggcaatg aatggttcaa aatgattctt acaaacgata aaggtgatca
     6541 attatttgta gaagatactt ggtcatattt gagtgaatat attgttagca ttaaaatcat
     6601 tgattttgta gcagataaag aaaaagagat tggagaaggt aaatctaatt actagctaat
     6661 gaatagcaac tttggaggat aagtcaaaag aataaaaaaa gaatactgtg tgtcattgca
     6721 gggatgtatc tttgcatgat agtatctgcg gttaccattt attggttagg aaatcagcaa
     6781 aacagtttaa ttgttgatct gggggagtta aaggttggat tacgaatgac tagatggctt
     6841 ttattagctc aaacagttgg tttagccata ttgacttgga tatgtgtgga aaaaataaca
     6901 tctaataata atcaagagat tgttgaatca acaaacctaa gaataagaat aactgaacaa
     6961 gcaattgaaa agcaaaaaga caaagaaaaa aaggaaataa tgtcaattta tttatctagt
     7021 ggagcatcat tttttcatga aatagattcg atgaaactga gtagtcaaga aatcattgat
     7081 agatggaaaa acatgcctct aggtggtgag ttaatcgttg aagatagtga tggttggtca
     7141 gcttataaga aggacgagat tgtagctatc acgattttgt ttgaatagta atatattacc
     7201 actagataat ctgaatatgt tgatctgcta gtgacgtacc aaatgcgttt aatagaacct
     7261 tggtcacagg cagaaattga taaagcagta aaattgaacc gattatcaaa atttaacgta
     7321 ttaggaaatg ccttttaaaa atcgcaatac acaaacagaa aaataccaaa aaattagcaa
     7381 tgaacgtagg taagaacatg acaataaata ataataaccc taacaaatat aatgctcttg
     7441 tccaatgcta tatcgaaaac aaaattacaa tgcaagaagt cgctgatgaa ttaggcaaat
     7501 caaaaagcct agtatcacac gactttaagc acaatgtttt tccgaacccg attgagctga
     7561 ggaaaaaatt tcaaaagaga gtttatcaag aaatagggaa gtggattgat gaagggaatg
     7621 caattgaccc tttaattgaa aaatatcctt ttttaaaaag taaaaataaa attcaaaggt
     7681 taattcgtca aggcgatatt caagctaaag aacctgtttt gacaaccaat gatttggcta
     7741 aaataaatct tcataaacag attattgaag actttgaaaa aaatctttca acaactgaaa
     7801 ttttaaaaaa atacaatatt ccgagaggaa agctaacatc gactcttttt agagaatatg
     7861 gagaatcggc agttactaaa gcaaaggaag tagcgaatga gagaaaaaaa caaaaatagt
     7921 agtcgatacc tagtagcgac tgtagttttt gtttgtgtat tgattttcct gtttgatttt
     7981 ttgcagattg gaaaaagcat aattggaaat acattgagtg caaaggaaat ggttgcttta
     8041 ggtggtttgt atgaaggaca tttgttcccg aatatattca tttctatcgt ttctcatatt
     8101 aacttgtacc atttaatttc taatttattg ttcttagtag ttcttgaaga agcactgaat
     8161 gatcaatatg agtggttaac actattgatt gttttaatca tatcaggact tctaggaagc
     8221 ttagttcctt ttctgttaca aagcaacatc gtaagtatgg ggcttagtgg aggtattttt
     8281 ggatggattg ctttatttgc gattgcatac atccgctttg atagtaagga acatttatta
     8341 ctaagtgttt ttatattatt gataatcggc attttactgg tatctacttt tgaatcaggc
     8401 gagacaaacg tcttgtcaca tgtaatcgga tttgtctgtg ggatgatctt gggatttatt
     8461 ctaaagaaca tttcattgat caagcaaaag aattaaaagg gagtgctaaa aatgccaaaa
     8521 aaaaaagaaa agaaaattcc agtgtttttg tctgtctcag aaatgttatc acttcaaata
     8581 ctgttaagaa aaaagattga aacaatagac gaagaagtta gaaataaaag ggctatgtac
     8641 gaacaacgtg aagcgctaag agaacgaatc cactattttg atgatataca cactaaaatc
     8701 tatgaagcac gtacaaataa cgaagacgaa agcaaattag gattagctca acaacgaata
     8761 tttgaatggc taaatacgaa tactgagcaa gaaccgttaa gagcaattag taatcttttt
     8821 gaatttggaa cgaagcattg gtttaacgca gtagaaaagg atgctgactt acaagaagcc
     8881 tttgacacgc taagtaagga tagcaaatcc atgcgagatt tattgattac ttatctaaat
     8941 gagcatgagt ttgaacaaac gaaaaccaaa aagaaaaata aagaatagaa agttgtgatg
     9001 aatcatgcta ttaaccctgt taggttattt atgtttaatt cttgcttttt ttggggcaat
     9061 cgctataatt attggtcaat ttcaaacaaa taatccgatt atgcgtaaca taggcattgg
     9121 aattgtgatt gtaaccttta ttgtgtttaa tgccactaag atttttggaa gtaacgataa
     9181 acaatttggt atatcgggag ttaataatag ttatgctcca gattctttgg gaagggtagt
     9241 aatagatgga acatttgaaa atgtaacgag ttataaaaat gtttcgctga cagtgaagga
     9301 agatggcaag aaagcatcag acgacgttct cgctactatt aagccgtctc aatcacaatt
     9361 tgaagtgttt tatacagtta aagagaatag ccctgtacac aagctgacat tggtttttaa
     9421 cgttgatgga gaagaatact ataaagatgt aaaaataaat aaaacatcaa cgaaaaagat
     9481 tatcgaatca gcgaaaaaag aagtagctac agaagaaagt acaactgaat caagttctac
     9541 tgtcgaagcg gaactaccag ataaaattga ttttgatcct gaaaaagtca aatcgtttga
     9601 atctgtttct ggttctatgg ctgatcggtt taacactgaa gtcttcaaag ctatcaaaac
     9661 tgttaaattg gatactgaaa agcaaatgat taatgttacc ttattcaatt cagttgaatc
     9721 tatggatcag gataaaaaag ttacggttgc aagagaacta ggaaaaagag taattggttt
     9781 gtatcgtggt actgctaaac cagattcaga cggtgcagat ttgccagttc atgtagttta
     9841 tgaagacggt aaagtttttg gagattctac agcaaacgaa gcattaacga ttaaattggc
     9901 taaatagcca gaaagttata ataaacaaat ggtgtcacac caataatata taggtgtgac
     9961 accttggaga tgatagctga tggtagtaaa taaagaaaaa gttagaaagt atgtgacttt
    10021 aaaaatagct tctgttaaat atttagaaaa gcgagctaaa gaagaagaaa gagcaagagg
    10081 taagaaagta acagtttcag agttgttaga agaattgatc gagaatgatc aaacaataag
    10141 acgtgcattt actaataggt c
//
LOCUS       DAZUKA010000101        10021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000101 DAZUKA010000000
VERSION     DAZUKA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.104"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            270..539
                     /gene="rpsN"
                     /locus_tag="ACG8PN_002066"
     CDS             270..539
                     /gene="rpsN"
                     /locus_tag="ACG8PN_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4862203.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            554..733
                     /gene="rpmF"
                     /locus_tag="ACG8PN_002067"
     CDS             554..733
                     /gene="rpmF"
                     /locus_tag="ACG8PN_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4862204.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            765..1142
                     /locus_tag="ACG8PN_002068"
     CDS             765..1142
                     /locus_tag="ACG8PN_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862205.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1163..1312
                     /gene="rpmG"
                     /locus_tag="ACG8PN_002069"
     CDS             1163..1312
                     /gene="rpmG"
                     /locus_tag="ACG8PN_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4862206.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1336..2319
                     /locus_tag="ACG8PN_002070"
     CDS             1336..2319
                     /locus_tag="ACG8PN_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4862207.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2349..2471
                     /locus_tag="ACG8PN_002071"
     CDS             2349..2471
                     /locus_tag="ACG8PN_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4862208.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2489..4012
                     /locus_tag="ACG8PN_002072"
     CDS             2489..4012
                     /locus_tag="ACG8PN_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4862209.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4036..4275
                     /locus_tag="ACG8PN_002073"
     CDS             4036..4275
                     /locus_tag="ACG8PN_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862210.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4468..4929)
                     /locus_tag="ACG8PN_002074"
     CDS             complement(4468..4929)
                     /locus_tag="ACG8PN_002074"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4862211.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5124..5342)
                     /locus_tag="ACG8PN_002075"
     CDS             complement(5124..5342)
                     /locus_tag="ACG8PN_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862212.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5460..7076
                     /locus_tag="ACG8PN_002076"
     CDS             5460..7076
                     /locus_tag="ACG8PN_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4862213.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7317..8657)
                     /locus_tag="ACG8PN_002077"
                     /pseudo
     CDS             complement(7317..8657)
                     /locus_tag="ACG8PN_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9095..9241)
                     /locus_tag="ACG8PN_002078"
     CDS             complement(9095..9241)
                     /locus_tag="ACG8PN_002078"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862214.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9280..9474)
                     /locus_tag="ACG8PN_002079"
     CDS             complement(9280..9474)
                     /locus_tag="ACG8PN_002079"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4862215.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9384..9716)
                     /locus_tag="ACG8PN_002080"
     CDS             complement(9384..9716)
                     /locus_tag="ACG8PN_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4862216.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3325 a         1597 c         1983 g         3116 t
ORIGIN      
        1 aaaagctact taaaacggta tttgtgtttg attggactaa taaaagtgaa ttcttatttt
       61 tcagagccta aactgcatat tcttttaaat gatttgatta tctctatgta ccgataaatt
      121 cgttttcaaa tagttatcta ttcatatgag ttttatttca tgttatttat tgttgacaag
      181 agcaactctt gttggtaaag tatcagtgtc aaaacgtaac gattacgatt tgaggtgtct
      241 aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt gctaaagcaa
      301 agaaacaaat ggcgatgatt gaaaagtatg cagataaacg tcaggaatta aaagccgcag
      361 gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt ctgaggcttc
      421 gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg tcccgtatta
      481 agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca agttggtaaa
      541 aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga aacggttaag
      601 aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga atggtgatta
      661 tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag taattaaagg
      721 agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa attatttacc
      781 cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa tcgctagata
      841 aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg ggtttaccga
      901 cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa ggcttatcat
      961 ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag ttatttcaat
     1021 gtatcgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt tcacaattgg
     1081 aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt gaagatcgat
     1141 aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg tcgaaactgg
     1201 agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt tagaattaaa
     1261 aaagtattcg ccaaaattgc gcagacgggc aatttttaaa gaagtgaaat agctaagtgg
     1321 taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt aggggctgga
     1381 aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga agaaattatt
     1441 ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt tcatagcgag
     1501 gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag tgatttatta
     1561 gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat ttcacaaatc
     1621 atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact tttaacagcg
     1681 cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga tagtgacagt
     1741 tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatggc agatcgcatt
     1801 tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca gagagaaatc
     1861 aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac gattcaaaaa
     1921 aaggattttt ataatttgaa aaaatttaat catcccttag tttttgaaag tgattcatca
     1981 gaaaatcact ataatggcaa atactatcat caacaccatc aagtacatga gtttaaatca
     2041 attctcttaa cttcagatca agacataaaa aaagattttt tactccaatg gattgactgg
     2101 ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc tttacaagag
     2161 caggatttta tagttgccat gcaagaggta aacgaacagg ttaattttca atacaccact
     2221 cgacaaaaag accaaaccac tattattcta atcggaaaaa tgttggatga agaaaggatc
     2281 aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtagt gattgggggt
     2341 aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa agcccgaata
     2401 ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat aaaatgaaga
     2461 aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac ttattgggat
     2521 agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag tatcgaatga
     2581 taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt tcaccaaaaa
     2641 catcgtcggc gatgaagcga acgtggattt aatggttcca gcgggatcag aacctcatga
     2701 ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt ttgtatatca
     2761 taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga aagctgggcc
     2821 taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag aagaagatca
     2881 tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg tttcacccaa
     2941 aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt atccgaaaaa
     3001 agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac ggttggatgc
     3061 tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacgc aacacgcagc
     3121 ctttggatat ttagctttgg attatggatt gaaacaagtt cctattgctg gactaagtcc
     3181 tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta aaaaaaataa
     3241 gatcaattat atttactttg aaaagaatgc taatgataaa attgcaaaaa ctttggccaa
     3301 cgaagctggg ataaaattgg aagtactgaa tcctcttgaa agtcttacaa aagaacaaat
     3361 ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt tagaaaaaac
     3421 aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg aaaagacagt
     3481 tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg attatacagg
     3541 cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag ttttcgatta
     3601 caaagcaaaa atcaataaag acatgacagc tgccgagtac aaagattatt atactactgg
     3661 ctataagaca gatattgata ctatcaacat taaagacaac accattgatt ttgtagtcaa
     3721 cggtgaacat catcaataca catacaaata tgttggatac aaaattttga attatgaaaa
     3781 aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg gtcgttttaa
     3841 atatattcaa tttagtgatc atggaattgc accaagtaag gcagagcatt tccatatttt
     3901 ctttggtgga gaaagtcaag aaaaactcta taatgaattg cataattggc ctactttcta
     3961 tccagcttca ttgtctaaac atgaaattgc tcaagaaatg atggctcatt aaaattcaat
     4021 ttttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa gaatattgat
     4081 gcttatcggc gttatcgatt tgcaaagcaa atgagattaa atgggagtac attagctgat
     4141 attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa acgggatcct
     4201 gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa acggcataat
     4261 agcttggcca attaattgat aataccagag tagatcattt ttcccttatt tggatttgat
     4321 ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca aagaagactt
     4381 cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt tcaatcatct
     4441 gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag tattctcgaa
     4501 tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat ctggaaaata
     4561 aaagggcata ttattcaatg cttctgtaac tgcgaaatga ttggagaatt cttttcctct
     4621 atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt caatcatttt
     4681 ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa ggaagcatga
     4741 ttttctgtct gtcaaagtca ctaaacaagt accaccagtt ttgccagcta caatatcggc
     4801 tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggacggccat ggatcgaatg
     4861 agtaaggcaa atctttccac gagactcgac atgatttttt gtatgtcttg tttttcctcg
     4921 atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg tatcgaaaag
     4981 atcattgcaa attgcacgtt agatcgtgat aaaactaatt gaaaagtcca tatgttcatg
     5041 cttcaaacga ttcgagattt cctctggcga ccactgaaaa tcgacaaata aatgacgaac
     5101 aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt ttcttcgata
     5161 agcataattt ttttccgctt tggaaggtga atagtttttt tcttgtttct tctcagttca
     5221 tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat agaaaatcct
     5281 tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag taagatgacg atactggctc
     5341 ataattattt tccttctagg agtgttctcg acaaacttat actagttgga tatcgattat
     5401 gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag aggttcccta
     5461 tgaaaaaaat acctattaca atcttaactg gctatcttgg ttcaggaaaa acgatacttc
     5521 taaatcatat tctgtacggt gatcacggaa agaaaattgc tgtcattgta aatgacatag
     5581 gggaagtaaa tgttgatggg gtattagtcg acaagaaagg ttttaaaaga actggagaaa
     5641 aacttgtttc gatgccgaat ggatgcattt gttgtacatt acgcgaagat ttgattgttg
     5701 aactagatca attagtgcaa ttttctgact tgaatcaaat tgttattgaa gcttcaggaa
     5761 tcagcaaacc aattccaatt attcaagcaa ttatattagc taaaaaccct ttagacattg
     5821 atttgaccca acgattagag attggtgcca ttattatagt agtggatgta aaacgttttt
     5881 gggataattt cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt aatgtagttg
     5941 aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt aacatattac
     6001 ttttaaataa tagtgactta gtgtcaacca agacgattta caaaattaac ggtctaattg
     6061 ctaagttaca gtccaatgcc aaaataatta ggacaattaa aggtgaagta gacttatcga
     6121 aaattctaaa tacaaatctt tttaatatag atgagacttc ctattcagtg ggctggttca
     6181 aagagttaga gcttggctat caaaatcata ttcctgaaac agaagaatat ggtatctcaa
     6241 gttttatcta tcgaaatcgg ttactccttg acgcacaaaa atttgcccat cttacggata
     6301 aaaattttcc tggcaatatt acaagagcaa aagggtttct ttggtttgcc caacatagtg
     6361 atttttcgac gatctttgaa acatcgggta gctataaaag tattgaatca ttaaattatt
     6421 gggtagctag tttacctaaa attgagtgtc aaatgattat acaaaaaaat cccgaattga
     6481 aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt attggtatga
     6541 agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat tctcttaatg
     6601 cagataattt ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg tttagccaga
     6661 tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc ataattattg
     6721 gcattttgat tattacctct tttattccct tatttgtatt ctctatagaa aaggagaatg
     6781 aaacaactga agatgctatc aaatatgcca tcgttcgaat tgatggcaag gaaatggatc
     6841 gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg aagggacaat
     6901 ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat agtcctgatc
     6961 aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt tgtttacccc
     7021 ataagttagt tattagtatt gagaaaaaag aaagcacaga ttactacatt tactaatctg
     7081 gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat agcaggggct
     7141 ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac aggcacaagt
     7201 atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt attttggctc
     7261 ttcgtcaatg agtgttggtg aagaaattca atagaataat cgagatactc acacggctag
     7321 acagctatct tgtctagccg tgttgttgtt ctggctttag tcagttgggt agtattctta
     7381 gttttcttaa aatattttcc tcgatataaa aagattcgtt ctcggtagtt ttgaaagttt
     7441 ctatagccat aggcaattcg ttttagtact ttgatatggt tattcaaaca ttctataggg
     7501 ccgttggaat agggaacgcg caacgctcgt ttgatttctg tttggtattt cttgaaggtc
     7561 gtgaacactg gttggtagtg ctctggcaac tccttgtagt cttcttttag gagcgcgacg
     7621 aattcaggaa catcttgtct tctgataacg gaaagaaaat cctggtagac ttcgtagcct
     7681 cgctttaagg aatcatcata agataatagc cgatcgacta tctctgcttc tgtcagatga
     7741 tctcgaaatg acggacgcca gtagcgatgc tgacctttca attcccacgc attcttttga
     7801 agtagtttcc agtatttctt taagtgcttc gaccgacgat cccctttttt aaaggtcttc
     7861 attacttgaa ttcggtggtt ggtaaaagcc cgaccaatgt gttggctaat atgaaagcga
     7921 tcggcgacga ttcgggcatt cggaaaacac tctttcacca atgtgcgata gggtgtatag
     7981 aaatctgaaa ccactagacg cacttgttca cggactttta tagggtagcg caagaaatag
     8041 tcgcgtaaag gattgagttg ccgattttcc acaatatcaa tcagttgatg ggtcttgccg
     8101 tccatcatga caaagctcat ggctcccgag acatttttca ctgatttgaa ttcatcgaaa
     8161 cacaccactt ctggtaacag ctggctcaaa gaaatggttt taggccgaag gctgcgaagt
     8221 attcgaatga cagtggtcgg agaaatgtgc ttcatacgtg cgatcaaaga catagaaaga
     8281 ggttcgctta atagctcaag aatggcttgt ttgactctgc gagcaatgga gcagcgacga
     8341 tcactaactg aatcttctgc taaaaaagtc tttccacagt gtttacattt gaagcgctgt
     8401 ttgttaatcc gcagaatggt cttgtagtta ctgacatcat tcaataagat ggatactttt
     8461 ttccagccct atttaatgat cgtttgttca ttttgatagt ggcgatgaaa gcattcctcc
     8521 ggttgatagg tcaatgtgcc atcaaaaaca aaacatgttt cgtcattgat tcgctctttg
     8581 cgaaaggcat cttcatgaaa gataagattt aggtctagaa tatctagtaa ctctttggta
     8641 aactctgaat aggacatctt gaaacactcc tttgaatggt tttcgtcgac tttattctaa
     8701 aggattttaa ggtgtctgtt tgcgtttatt agaaaaaaga aaaagtattg gcaacagaag
     8761 gcgtcaccaa cactagatat tatagatccc ttattttctt cgcttcaaat tgtcaaggag
     8821 tcatcataaa ttttgtatgg attcttttgt ttgggtagta gtcttctaaa taatcttgga
     8881 ttaacacctt catctctatt ttacttttat atttgttgga gtcgattaat tctcatttga
     8941 ttgatcgatg aaacgcttca attactgcat tgtcaacagg agtcccagtt tggctcactg
     9001 aatgtgtgat gtggtggtcg ttcaacagct cttgataaat aaaagaccga tgctggctac
     9061 ctctatcgga atgaattacc gtaggcaatt ctccttaata aatttgtaat ggacggacga
     9121 caatctcttt tttcatgtgt gaatctacca tatagtaacg tagaatacga gttgctaagt
     9181 caatataggt ggacaaatag aggcgaccgt cattacatgg aatgtaagta atatccatca
     9241 cccaaacagc atcaatttgg ttctgttcaa attatcgatt caacattttt ttaccatttc
     9301 tttgattgtt ttttctctac catgagaaga tttgcgtcga tgaaatcctc ttgtgcataa
     9361 attcatttca cgcataagaa cagctacaac acgtttattg atttcgattc cttcatcgta
     9421 aagtttttga gtgatccctg gagatccgta tcgatctttg tgttcgtaga acactcgtct
     9481 tcctctttta gtcgtttatt ttcctgttca agaatgtaga aatctgcttt atcaggtaat
     9541 ctttttcctt gtccagcaaa agcatttttc ccgtatcgac gatattctga aacccatcgg
     9601 gaaattgttg gacgagagat attgtattct tcagcaattt tcttggtagt tacttttttc
     9661 tcaaggatat cctgtgaaat ctttagttta aatttacgat catatgtttt tctcaataga
     9721 gttcattcct tccggtaacg taactgatag tcaagaaaag tgtatcatag cccaacttag
     9781 tatcagggtt tgtcaactaa actgtggaag ttgatagata agagttttta acaaccaaaa
     9841 ttctcttgac tatttttgaa atcagacaaa ttttgattgg acacatagtt gaataaatct
     9901 accgtcgtta cggaaggtaa atcgcatgct tttcaattct agaggagaag tcctcgttga
     9961 agtaccatag cccagttctg gattcgacct cctgcccact tcatacgtaa ataaagtaat
    10021 t
//
LOCUS       DAZUKA010000102         9688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000102 DAZUKA010000000
VERSION     DAZUKA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.105"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(560..1600)
                     /locus_tag="ACG8PN_002081"
     CDS             complement(560..1600)
                     /locus_tag="ACG8PN_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4862217.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="ACG8PN_002082"
     CDS             complement(1949..2275)
                     /locus_tag="ACG8PN_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862218.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="ACG8PN_002083"
     CDS             complement(2262..3050)
                     /locus_tag="ACG8PN_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862219.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="ACG8PN_002084"
     CDS             complement(3418..3708)
                     /locus_tag="ACG8PN_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4862220.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="ACG8PN_002085"
     CDS             complement(3844..4023)
                     /locus_tag="ACG8PN_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862221.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="ACG8PN_002086"
     CDS             complement(4020..4370)
                     /locus_tag="ACG8PN_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862222.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="ACG8PN_002087"
     CDS             complement(4363..5688)
                     /locus_tag="ACG8PN_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4862223.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="ACG8PN_002088"
     CDS             6138..6752
                     /locus_tag="ACG8PN_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4862224.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7159)
                     /locus_tag="ACG8PN_002089"
                     /pseudo
     CDS             complement(6917..7159)
                     /locus_tag="ACG8PN_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7363..8682)
                     /locus_tag="ACG8PN_002090"
     CDS             complement(7363..8682)
                     /locus_tag="ACG8PN_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS4862225.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8816..9424)
                     /locus_tag="ACG8PN_002091"
     CDS             complement(8816..9424)
                     /locus_tag="ACG8PN_002091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862226.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
BASE COUNT         2974 a         1735 c         1487 g         3492 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgcgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatcc ctgaataatt catagcttta attctttggc acattttatt
     7201 gaaatatgta tcactaatat ccctatcaac tgattttgac taaaaagtta cttcaaacat
     7261 tcactgttca aaaatattga ggaataaatt ttgggtgtaa ggcattttga gcatacttct
     7321 ccctggataa cttcccagta aaaaaatcgt tagattgttg gattagatcc attgactggc
     7381 tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga tacacatgat aactagataa
     7441 tttgagatag actcgtctac ccgtttggac taatttccca gcaactttca gaaacttcaa
     7501 tcgaatagaa tgaatggtca ttcccttttg gacttcttca aagccaatcg ttcttaagaa
     7561 gttgactaag ttgtaagcta tcaagctgag catcattcgg acatgattct ccaaaaagcg
     7621 aggactgtct gttttgtcaa agtaaaagcc agctttcgct tctttaatga agttctccat
     7681 tgtgccacgt ttggcataga tagaaaagat ggtatcagga gaaacatttt ctgatagatt
     7741 cgtcacgata aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg agcgtataca
     7801 cacgcgacga ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt actgaacttc
     7861 tcgttcttcc cacttttgat tatcgccata aagtactgat ttctcagcta tttgacctag
     7921 tctacgatta ttcttcagtc gaataacata atgactcttt tttgattcac acgaatcata
     7981 cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga
     8041 gtgattatag tgctctaata aaggtgtgag aaactccttt acgccttttg atgtatattg
     8101 atttcctgaa cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa
     8161 tggatggtaa ccataggttt ggtagtgtgt attataattc gtttgttctt ggtgaccaaa
     8221 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
     8281 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
     8341 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
     8401 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
     8461 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
     8521 gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc
     8581 tttcactagg acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat
     8641 atttgaattg aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt
     8701 ttggttggtt tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg
     8761 gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa
     8821 agtatgaatt attcaggcta tcattaattt tataaaaaaa attaaatgtt tttagcactg
     8881 ccatataaca ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca
     8941 atttattacg tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa
     9001 tattaaagta ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat
     9061 tctcaaatat tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt
     9121 cacaggctgt aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt
     9181 taaggagtat aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt
     9241 gagcttcaga agttatatct ctaattatat agttaacgga attaggaagt gtctttaact
     9301 ctaatgaaat ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg
     9361 attcgtcagc aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa
     9421 tcatttcagc aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta
     9481 tatcagaatc aataaattct atttttttat tactatatcg tcatcattat cttgaccatt
     9541 tatgtttatt gttccctgag tggggagtac ttctttcaaa ttcaagttaa atttatattc
     9601 cattacttct cctccaatat ataaaaatta cttaactgta tccaaattta atatgtaaat
     9661 aaatcattaa tttttagcta aattctat
//
LOCUS       DAZUKA010000103         9648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000103 DAZUKA010000000
VERSION     DAZUKA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.106"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8PN_002092"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8PN_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4862227.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG8PN_002093"
     CDS             1995..4661
                     /locus_tag="ACG8PN_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4862228.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG8PN_002094"
     CDS             5025..5777
                     /locus_tag="ACG8PN_002094"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4862229.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG8PN_002095"
     CDS             complement(5799..6953)
                     /locus_tag="ACG8PN_002095"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4862230.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG8PN_002096"
     CDS             7138..9579
                     /locus_tag="ACG8PN_002096"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4862231.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3111 a         1737 c         2067 g         2733 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttt
//
LOCUS       DAZUKA010000104         9641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000104 DAZUKA010000000
VERSION     DAZUKA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.107"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1057
                     /locus_tag="ACG8PN_002097"
     CDS             83..1057
                     /locus_tag="ACG8PN_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862232.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1079..1771
                     /locus_tag="ACG8PN_002098"
     CDS             1079..1771
                     /locus_tag="ACG8PN_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4862233.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            1737..3245
                     /locus_tag="ACG8PN_002099"
     CDS             1737..3245
                     /locus_tag="ACG8PN_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4862234.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            3319..4422
                     /locus_tag="ACG8PN_002100"
     CDS             3319..4422
                     /locus_tag="ACG8PN_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4862235.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            4425..5762
                     /locus_tag="ACG8PN_002101"
     CDS             4425..5762
                     /locus_tag="ACG8PN_002101"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4862236.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            5749..6627
                     /locus_tag="ACG8PN_002102"
     CDS             5749..6627
                     /locus_tag="ACG8PN_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4862237.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            6624..7547
                     /locus_tag="ACG8PN_002103"
     CDS             6624..7547
                     /locus_tag="ACG8PN_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4862238.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            7544..7954
                     /locus_tag="ACG8PN_002104"
     CDS             7544..7954
                     /locus_tag="ACG8PN_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4862239.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            7958..9526
                     /locus_tag="ACG8PN_002105"
     CDS             7958..9526
                     /locus_tag="ACG8PN_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4862240.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         3132 a         1589 c         2101 g         2819 t
ORIGIN      
        1 aaaatgatta tatacgtaat tcagatagtt atctatcatg aagtcataag cagcaaacat
       61 tcacaaaact aaggaggggc ttatggaaag tgtaaagaaa caagaaccat ttcaactgac
      121 gctatcgaaa aaagaagaaa agctcagaag acgaaaaaaa ataattgcag gaatcaaaac
      181 gaataaattt ttatatgtga tgattctgcc gggagtcctt tatttcttga tttttaggta
      241 tcttcctatg gggggactcg ttattgcttt tcaagattat caaccatttt taggaataat
      301 gggaagtcct tgggtcggtt tcaaacattt cattcgtttg tttacagaac cgacattctt
      361 tacattatta agaaatacgt tgatcttgtt cggattaaat atcttcgtgt tttttccgat
      421 gccaatcatt ttgtctttac tattaaatga agtaacgaac aaacgattga aaaacgggat
      481 tcaaacgatc atttacatcc ctcacttcat gtcttgggta atcattgttt cgattactta
      541 tgtatttttg aatgttgacg gaggggtatt gaatgaattc cttgcaacaa tgggatttga
      601 aaaaatcagt tttctaacat cccctgaatg gacacgcaca gtatatatcg gacaagtgat
      661 ctggaaagag ttgggctggt ctacgatcat ctacctttca gcgataactg cagtcgatac
      721 acagttgtat gaagctgcag aaatggatgg agcaggacgc ttacgcaaaa catggcatgt
      781 tacattgccg gcaattcgtc cagtaatcat taccttgctg attctaaaaa tcggtcagac
      841 attagactta gggttcgagc atatgtactt gttattgaat tcattgaatc gaagcgttgc
      901 agaaattttc gacacctata tttatacagc tggattgaag aatggacagt taagtttcag
      961 tacagcaatg ggattgttta aaggggtcgt aggattgttt atggtaattt tagctaataa
     1021 gattgccaaa aagctaggag aagacggcgt ttactaagat agaaagagag ggaaagatat
     1081 gctgcagtca ttagataaaa taaacggaag gctaaatcgt attttacagt tggtttattt
     1141 gaattttctg tggggcggtt tagtcatcgc aggtttggga gtttttactt tcggtcctgc
     1201 atcgtatgcg atggttgcag tgattagaaa atggctgcgc tcaaaagaaa cgtttccttt
     1261 gacgaaaacg tacttcacat attttaaaga aaactatcgt gaaaccttgc tgatgagctg
     1321 gatatatggc gccatcggga ttgttttact tgtggatctt tctttcatac acaattggta
     1381 tcttaggatc gctttgtata ttgctgcgtt catatatgtg ctctcagcac tctatatttt
     1441 cccaatcatg gctcattatg attggaaagg atattttttc aaaatgaaaa tggcattttt
     1501 attcggattc tcacatcttc attactcatt agtgttgata ttaagtatgg tagggattta
     1561 tctgatgatc atccaaacaa tgccaggtgt tttaaccttt atcggtggca gtctcttctt
     1621 ttccgcagtc acgtggacag caaattctat attttgccat ttggaatgga agaactcata
     1681 tacagaaaaa acagaagaaa acaaaaatat tattaaaaca gtgaaggaga aaatttatga
     1741 aaaaagcaac gctcgtaaaa ttaggtctta gttttgcagg aattctagca cttgccgcat
     1801 gcggcagcag ttcaagtaac agttcttcag agacagaaga tggaaaagct aaaatcactt
     1861 ggttaaatat tttgcatcat gcttcaccgc ctacagacac gattgtgaac ttgatcgaag
     1921 aaaaaaccga tacggaactg acattcaact ggattcctga tgcttcaaaa gaagaaagat
     1981 taacaactgc attggcttct gatgaactag gagatatcgt tacacttcaa attgcgatgt
     2041 tgaaaaactc ttctgtccgc aatgcgttaa aatcaggcgt attctgggat gtatcagaat
     2101 atttaaaaga ctataaaaat ttaagtcaaa tttctgaaga tagaatcgaa gctgcgagta
     2161 tcgacggtgc cttgtacggc gtaccgatac aaaaagacta cgctcgtgct ggcttagtca
     2221 ttcgaaaaga ctggttggat aatctaggac tagatgttcc aactaccatg gatgagcttt
     2281 atgaagtagc taaacaattt acagaaaacg atcctgatgg aaatggtgtg gacgacacag
     2341 ttggttttgg cgatcgtgcg cctagtgata ttcgttatac cagctttaaa cttttcactt
     2401 cttattttgg agcaccaaat ggctgggctg tagacgataa tggcaaattc actccagaat
     2461 ttgatacaga agagtatatg aaagctttga atttttcaag agatctattt gagaatggct
     2521 atttggcaca agactttgca gttacccaaa agactgatca gcaagaacaa tttgcacaag
     2581 gaagaactgg aatctacact ggaatggtgg atatcaagaa tctgaagacg atgggtcaag
     2641 gtattcaaga tgatatggaa ttagtgcctg taaacaagat ctcagatggt gaaggcggcg
     2701 gttatcatgt ttggtcagaa ggaaatggta ttggcggctt gctagcattt cccaaatctc
     2761 aagtaaaaga cgaagcacaa ttgaagaaat tacttcaatt tgttgatgat cttttagaag
     2821 aagatgtgta taaattgatg actggcggta tcgaaggaac gcattatgaa ttgaatgatg
     2881 atggttctat cgaatttatc gatatggatc tttggcaaca agaggtgcaa ccgttatcaa
     2941 gctctcgtcc tagtgaagtg acgttctctt tcaaagatac agaccctgaa aaagaattct
     3001 ctaatcaatt gatcaaagaa aatgaagaat ttgctgtaat ggatccttct gtgccattgg
     3061 attcaccgac gaacaatgaa gtaggatcag aaattgaaaa aatcgtcatt gatgctacgg
     3121 tgcaatacat catgggacag cttgacgaag atggattcaa aaaagctgta gaagactgga
     3181 aagcacaagg aggcgatcga atcacacaag agtacgagga agcatacaaa gcagcggcac
     3241 agtaatatag acgattggga caaatacttc ttggtatttg tcccaaatcc tattttaatt
     3301 tttaaaggag agcaaataat ggaaaactgg tcaaagaaat tagcaaaaag tatcatggaa
     3361 agaacacctc gcttatatga agaaaagtgg tataaaggaa aatggtctta tgactatggc
     3421 gtcgtactta aagggttcca attactttgg gaacgaaccc aagaaaaaat ttattttgat
     3481 ttcataaaag acaatatcga ctatttcgtg caagaagatg gaacgatccg tggctacagc
     3541 gttgaagaat acaatatcga ccatgtaaat acaggaaagc ttttcttttt attgtacaaa
     3601 gaaacaggcg aagaaaaata taaaaaagca gcagaactcc tttcccagca gttggcaaac
     3661 catccacgca catccgaagg agctttttgg cataaagaaa tttatcctta tcaaatttgg
     3721 ttggatggct tatatatggg ttctcctttt tatgctgaat ttatccagaa ttttagtgaa
     3781 gaaaaagatt accaagatgt tttgagacag tttgaaatca gttataaaca tctattcgat
     3841 ccaaaaacaa atttgctgat tcatgcatgg gatgaaaaga aggtccaacc atgggctgat
     3901 ccaaaaacag gtctatccca tcatttttgg agccgatcga ttggctggta tttgatggct
     3961 gcagcagata cctatgaatt acttcatgaa gaaacagatt gttctttgct gaaagaaatt
     4021 ctagaaaaga cactggctgc attacttcca tatcaagaag aaaacagcgg gacatggtat
     4081 caagtaacaa cagaaacaaa acggaaagga aattatctgg aagcttccgg cagtagtatg
     4141 tttgtttacg caatggctaa aggaatacgt ttgggactgc tgaacaaaga agaatggctt
     4201 ccacaagtga agaaatcaca tcaaggatta cttgatgaat ttgttctgga aacaaaagaa
     4261 ggctggctga acttgaataa aaactgtcaa gtagcagggt taggcggagc agatcagcga
     4321 gatggttcat atgcttacta catcagtgaa ccaattattt gtaatgatca aaaaggcgtt
     4381 ggtgcattct tgcaagcatt gattgaagtg gaggcattat aaatatgtat gatattttga
     4441 catttggcgc atcaattgat gaattgaata ctgaagcaat acaacaagcc attgatgctg
     4501 cagcaagtga cggaggcggg accgtagttg ttccagcagg tgaatttcta acaggagctt
     4561 tatttttaaa aagcaatgtc gaacttcatt taagcgcagg agcggttctg aaattctctg
     4621 acgatccaaa agattatcct gttgttcatt cacgttggga aggagtccat cgaaaagttt
     4681 atgcttcctg tatctatgct caaaatgtag agaacatctc tgtcactggt ttcggtacat
     4741 tagatggaaa tggaaaaaaa tggtggcata cttttagaaa tgaaccagat aatctcgctt
     4801 atcctcgtcc taaattgatg agtttccata actgtcatcg aattactgtg aaagatatta
     4861 aattgatcca gtcaccaagt tggacgatca acccgattct ttgcagtaat gcaacgtttg
     4921 ataacttgac gatcttgaac ccagcggatt caccaaatac agacggaatt gatccagaat
     4981 catgcaaaaa tgtgcgtatt agtaattgcc atattgacgt gggagatgac tgtattgcaa
     5041 tcaaggcagg gactgaagat acgtacgaaa ggatcgcttg cgaaaatatc acgatcacaa
     5101 attgcacgat ggtccatgga catggcggtg tcgtattagg aagtgaaatg agtggtagta
     5161 ttcgtaatat taccatttca aattgtatct tccaagaaac tgatcgagga atacgattga
     5221 aatctagacg aggtcgaggc gggatcgttg aagatattcg agtatccaat atagtcatgg
     5281 ataatgtgat gtgtccattt atactgaatc tttattattt ctgtggtccg cgagggaaag
     5341 aaccgtatgt atgggaaaaa aaggcttatc cgatagatga acggacacca gcattcagaa
     5401 ggatccattt ttcgaatatc acggcaagga atgtccatgc ttctgcaggt tttatttatg
     5461 gattagcgga acaattcata caagaaatca catttgacaa tatcgatgtc tcgatggccc
     5521 aagatgcagt tcccggtgtt cctgctatga tgacaggaat agaggaaatg agcagacaag
     5581 gattttatat tggctttgcg ggtaaggttt cctttaacca gatgtcgatt gaaaatcacg
     5641 agggaccagc atttctatta gaaaatacta aagaaatcag tattgagaaa ttccaatcaa
     5701 atcaatcaga aaatatggat gaactggttg ttcgtaaaaa ctttgcttat gcagttgaat
     5761 gattttttag gtgctccatc tagcagacat acccctagtg gaaaacgatt ataccgagaa
     5821 aaacaaaacg gttttatgct tgaatactat caattgtact taaatgaatt ggaaacagta
     5881 ccagccctct atgcttatcc agaaaaagaa ggaccgtttc cgccggtcct ttacctgcat
     5941 tctcacggcg gagatttttc agtcgggaaa agtgaattga tccatggtgc tccttactta
     6001 gcttctccat cttttgcaga agtactgaca ggaatgggat atgctgtttg gtcgattgat
     6061 gcttggggat ttgaagaacg cggaggtatt tctgaaagcg agttgttcaa acaattttta
     6121 ttaacaggaa aaacgttatg gggaatgcgt atttatgatg tgatcagctt gatcgattat
     6181 ttggagacaa gggaagacac agatactcaa cggctcgcaa cgattggtct ttctatggga
     6241 ggcttattaa gctggtgggt gacagcattg gattctcgtg tcaaagtttg tattgatcta
     6301 gcggcacagg tggacatcga aatacttttg ctacatcgcc gcctagatca tcatgggttt
     6361 tattactatg tgccaaatct attgacagct tatacaacgt tagcaatcca agaaaaaatc
     6421 gctccgcgcc ctcgtcttag cttagttgga aaaaacgata cgatgtgctt ggacgaaggc
     6481 gtactgaagc tgagaaaagg gctggacaaa aagtatcaat caatgggtag tccagaaaac
     6541 ttttcttctt tttctttaac aggcggtcat atggaaacac aagaaatgag aaatatttgg
     6601 aaacaattca taagggagca tttatgagga tgaaagacaa actgataatt ggaaagaatg
     6661 ctatagatac cccgacggat tttgaaacta tccaagaagg aatcgattat ttggcaggcc
     6721 tgccggatag ccatccaaag gtcttgatag taaaagaagg aagatatgat gaatatatag
     6781 aaagcagatt atccaatttt aaacttgtgg gagcaggaac agtagttatc tccggtaatc
     6841 gttttgcaaa acaggagtat cttgacggta gccagttagg gactttccga agtgctactt
     6901 ttttcttaga aggatcgaat gtcgtattag aaaatcttca catcgaaaat acagcaggtc
     6961 caggagaaaa ggtaggacag gctgttgctt tattcaattc aggagatcgc gtatctttaa
     7021 aaaactgtcg actatctggt tatcaggata ccttatgcac gggtccgttg cctgatttgc
     7081 aaaaagatgg tactcctttt tgtacaccgg cttctgccaa tcctgagtat tgtcgtcaga
     7141 cctatgaata ttgctggatt gaaggaacgg tggatttcat atttggtggg gcagatgccg
     7201 tttttcatca gtgtcatatc catagcttag ccagtaaaaa tcctggatac atcacagcag
     7261 catctacgcc gaaaaatcaa aaaacagggt atcgttttga aagctgtacc ttgactgcag
     7321 aacctgatac tagaaacgtc tatttaggaa gaccgtggcg tccgtatgca aatgtcttat
     7381 ttgaagcgtg taagataggt gggcatgttc acccaactgg ttgggataat tgggatgaca
     7441 agaaaaatga acagactgtc aaattctctg aacgtaataa caattatgaa acatcgatca
     7501 aaagacctga atggatacat ttagaaaagc agggggttca gaaatgaaaa gaaaatggga
     7561 actattgcta ggaatggtcg gtggcagtct atctttgatt ttctttggcg ggttagcagt
     7621 tactttaagt aatatgtctg cgtcagagtt taaaaaaagt tatcagtcgc ttgctgtaga
     7681 tcattcaact ttgtctttag aaaatacatt tggattatta caggacatga ctggtctgtt
     7741 tgccgttgtg ttgtttattt cacttgcttt tttagcagta gcattgtttt tgactgctaa
     7801 aggaaaatac ttaacaactg ccactggttt gtattttata acaggattca tattgttgat
     7861 tggcactcag tttattgcgt ttccttttgc atttttttat ttcgcagctg gggcattcag
     7921 tttatatcga gtaagaatga gaaaaggagc ctgaatcatg cagactatca aagtgaaaaa
     7981 tcctatttta agaggattca atccagatcc taatatatta agagtgaaag agcggtatta
     8041 cattattgtc tcctcttttg aatggcttcc aggaatccga gtatatgagt cgaaaaattt
     8101 agtcaactgg tcacatacaa cggatatttt gaccactcaa gtatctttgc aaggaaatcc
     8161 tacaaatgga agtatatggg cgccacaaat cagttatgct gatggattgt tttatcttgt
     8221 ttacacagat gtcaaaagtg caggccgtcc gtttaaagat gtccacaatt atttgatcac
     8281 tgcatctgag atcacaggtc cttggtccga accaatttat ttaaatagta acggctttga
     8341 tccttctttg ttccatgatc ttgaaacggg aaaaaaatgg cttgtcaatg aactatggga
     8401 ttatcgtctg gacacaccga ataaatccag cggtatcgta ttacaagaat atgatgcaga
     8461 gaagaagaag cttgatggtc ccatctataa aatatttgca ggtacagaat tagcgaaaac
     8521 ggaggcacca catttgtatt ttcgtaacgg gtattattat ttgatgacgg cagaaggcgg
     8581 tacaggaaga ggacattcag ttacagtttg ccgagcaaag aatattactg gtccttacga
     8641 gcttgatcct ggttttccta tgctgacagc tagtgacaaa cctgcttcta cattgcaatg
     8701 tacaggacat gggagcttag tacaaacccc gtcaggaaga tggtacatga catatctttg
     8761 taccagacca ttaaacggtg cggctatttt gggaagagaa accgctattc aagaagtgta
     8821 ttggacgtcc gacgattggt taagacttaa agatagccag acaactccga tggaagaaat
     8881 aatgattgag acaaatgaag aggtacaaca agaaaaagat catcagttta tggatacatt
     8941 tgaaggagag ctaaagaaga actggaatgc cagacgcatt ttgccaaatg ctgattggtg
     9001 tgatacaaaa tgtagaccag gttttttacg tctgatttcc ggtgaatctc ttcaatctac
     9061 cttcgaacat catttgttag ctattcggca aacggatttt acttttgatg cggagacagc
     9121 atttgaatac atgcctaata attttaatca gatggctgga ctggtactgt acttaaatga
     9181 atccaattat atttattgtt atgttacttg gaatgaggac aagggaaaat gcctacgtat
     9241 gatagaatca gaaaacggag tagcacaaat agaagattgg acgattccac tcacagaaaa
     9301 gaagactttt ttgaaagtca ctgtaaagaa agagacagca cagtttcatt tgaaagacgt
     9361 atcaactgaa gaatggaaga aagtaggtcc gcaaaaaaac atgtgtatac tctcaggagg
     9421 gtttactgga aattttatcg ggatagcagt gcatgatcta aataaaaaaa gaggaagtta
     9481 tgctgatttt gagtttttca agtatgatgg aaaagatcat ctgtaattta attctttaat
     9541 aaatgaggtt cactaactta ttttcactag ttagtgaatt tttttgtttg tatatattgt
     9601 tatgtaaaaa aagttatcaa tcttatgcat caatgttttt a
//
LOCUS       DAZUKA010000105         9627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000105 DAZUKA010000000
VERSION     DAZUKA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.108"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            779..1582
                     /locus_tag="ACG8PN_002106"
     CDS             779..1582
                     /locus_tag="ACG8PN_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4862241.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2167..2622
                     /locus_tag="ACG8PN_002107"
     CDS             2167..2622
                     /locus_tag="ACG8PN_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862242.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2741..3799
                     /locus_tag="ACG8PN_002108"
     CDS             2741..3799
                     /locus_tag="ACG8PN_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4862243.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3841..5790
                     /locus_tag="ACG8PN_002109"
     CDS             3841..5790
                     /locus_tag="ACG8PN_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862244.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5793..9434
                     /locus_tag="ACG8PN_002110"
     CDS             5793..9434
                     /locus_tag="ACG8PN_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFS4862245.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3451 a         1384 c         1964 g         2828 t
ORIGIN      
        1 aaaaacaaat atttttctag tttttagaaa taaattcaca tactttttct ttttttggat
       61 atattcggaa taccacatcg ctttttgtcc acgtttgcat cgctttttgt ccacattcac
      121 atcgcttttt gtccatgttc gcatcgcttt atgtccaccc taaaatctat ttaagattga
      181 tataaagcct ttttggaggt tgctaaagtt ttaaagatta aaatatatat tataaatata
      241 aataaaaggg gaagctgaag cttcctcctt ttcctttttt tcaattattc aaagttacac
      301 aagcaaaata aactcctaga tagtgttagt tttttttaag attttttatt tttaagattt
      361 tgaaataaga gaggagtaat cctcctttat taaatatctg ttaatatatc tgttaaatat
      421 ctgttaatat atctgttagg ggaggcttaa tctcttggga gagtaagtat taaaggggta
      481 taaaggtaac acttttggac agtaaaggta acaaaagtgg acagtaaggg taacagtttt
      541 ggacagcaaa ggtaacaaaa gtggacattt gaggtatcac actaggataa gacgttacct
      601 tcggttttct tgatttggaa ggcgaaaaaa tagaaaatta gattagtaac aaatctttgt
      661 tgactgttac ctttagttgt gatttaattt aggtaacaag cagaaaaaat tgtttccttt
      721 aattagtacc agattaacat gattaaagga gcgcaaaaag aagattggag agtaatcaat
      781 gtctcaagaa gttaccgaaa agattattga tagtgatgta gatttagtat cagaacaaac
      841 aaatgcaatt gtgcgttata acaatggtct gaatacaaca gctttgcgag gatttaagcc
      901 tgttgaaatg gatgtgtttt gggctatttt atcaaaaatg aaacgtcaag gaacagcaga
      961 agtaactttt gatttcaacc tctttcgtta tttggtcaaa tacgacagaa gagaaaagga
     1021 cagtttttat tctgtgttaa aagatttgag tgacaaacta ggtaatttaa cctaccgttt
     1081 tgaagattca gacgtctatg aacagctgtg gctttttcaa aaattccgga tagataaaca
     1141 gaaggaaaca gttacaatta aggcaaatga ggaatttgaa tttatcttaa actcaattgg
     1201 caaaaacttt actcgttttg aacttgataa ttttacaaaa ctaagaagta gctacacaaa
     1261 agaattgtat cgtcatctca tgcaatacag aaataaagag tggcgaaatg gttattgggt
     1321 agtcaaagtt gaagatttta gaagagcatt aagtatacct tcaagttata gaatgacaaa
     1381 tattgataaa agaatactag agcaggcgaa agaagaattt ttagctcctg atgaaaacgg
     1441 aatacaggta tttgaaactt ttgattatga aaaaatctat gcaaagaaag gaagaagagt
     1501 tgatcggctc agattcactt ttagtgagcc agaatcaaac ttgccaacca tttcaatgca
     1561 taattggtta gaagaagatt gagaagaagc aggttgtaat tttaatacaa cctgcttctt
     1621 taaaaaagta tgggtaacac ttttggacaa taaatgcaag aagttttttt gtaagcaagt
     1681 ttcagtctaa ataatatttg aaagaatggc taatctatac aaattttaac aactatgggt
     1741 aacacttttg gacaagtcaa gaaaaaaaac aatttttctt aatttgttga tcgactaaag
     1801 gtaacacttt tggacaacac agaataaaaa gtccggtgat atcagttttg gtaggaaaca
     1861 tcttaatatc tttgtgagat tgaaggtaac atttttggac aagtcaacta tttgttaaaa
     1921 gttaatatag agtaattaga taagtttttt cttttcgtct gataaatgga taatattagt
     1981 tattttattg ctaattagtc tttcatgatg actgaaatag ctaaaataaa tatctcgttg
     2041 gataattgct gatactttaa gggatatcta aatttgctga ttgatttatt tatgttatta
     2101 tgatattata ataacataaa tgtgttacgc ccgaacctca ttttaaaaaa atgaaggggt
     2161 gagacaatga atgctattaa ttggcatgaa tttgttaact atatggagag cttactagat
     2221 gatctaaaaa gttttagtat tgctgatgca gtaaagcatg ggttaccagt tagtacaacg
     2281 atgaaattac gaggattgaa aattccagat gaaattatta agctgagaac taaaacaatt
     2341 attgaaaccg caaaagtcgt tgacaatatg aaccatgaac taaaaggcaa gaaaacaact
     2401 tacgagaaag attttgaacg ttttttaaaa tctgttattg gaaaatatct aggtaaaaaa
     2461 tctttcagtg aattgagaga tgaagagatt gtacctactc caatggttag caaaatccga
     2521 aaaaacaaag ccgaggggaa ttactatctt ggtacaatag caaaaatcat tgattatttg
     2581 gaaaataaag agcaaaagaa aggaacgaag aaaggaagat aatttttgtg ttaaatatta
     2641 atggggaaga atataccgaa atgattcaaa atcccgaacc tagaattacc gaaaaaacgt
     2701 ctgattgttt gctcttttat gaaagggggc agacaaacta atggaaaaaa acgtcaattc
     2761 aacatctacc aaagatcaat acagtattat tggagggttg cttggtgata aggataagag
     2821 aacacgtcac ggattacgca ttatgcttgc aacttttcta ggagtgcttt ttttattaac
     2881 aggcttgggt agctacattt atcaattagg aaactataat tattatctcg tgaacaatac
     2941 aacacctata ggctcttcat tggagtttgc tcgatcagga gcagagatca ctatttcaga
     3001 tgtgtggaca gatcaaaaca gagatgtaac agtagtaaaa ctcggttact cgcaaaacgc
     3061 tagaaagttg ttatctacgc aaggaaaaaa ctacaactta tatatggcaa cgaaagaaaa
     3121 agatcgtcct aaaaagcttg aaatgagcta tgggatgtta agtactgatg gggatgcttt
     3181 cttattccta aaaggtaaat tagatgaaag agcttatcaa gtttttattg caaaccaagt
     3241 atcactaatt tctggtgatg atacagctga cgatgatacc aaagtcaatg tgaatgaagc
     3301 agattcagtt acaaaagcac tttctcaata ttctatgaat gatgttaata atgaaggagt
     3361 aatttctttt gggaaaaaag caaaacctgt taatactcct gataatatca atttcagaat
     3421 aaatccatat tcagattcaa ctaatgtcta tcaaggaagc tttcttacca aagatggcga
     3481 tattgattat tcaaaagttg tagcagttac aagtaagaat aatgttgtaa gtaagttaga
     3541 agatcagtta aatgcttcaa atactaacat caagaaaatt gaagctacaa aagctgagta
     3601 tctggaacgt ttacaacaaa atcctgacga taaagattcg caagaaagta ttcgtcaact
     3661 agacagtcga atggagaccg aaattaaagc aatcaatgat acacaaaaat tgattgatca
     3721 atacaaaaat gctgacttta acaaagattc ttttggagac atgaagacga catttaagta
     3781 cctaactctg actgactaga agtaaattat tcttatttta gaggaagtga acacaaacag
     3841 atggaaaata gtaccgagtt ttttaagaat attcaaactg aatttaatca tgctctcaca
     3901 ccaatgaaac caccgaaaca taagttagtg ggacgtgatg acgaactcca tgagttgtca
     3961 ctgatcttga atagaccgga aacaccgcaa gctatcttgt taggtgaagc cggaacaggt
     4021 aaaacagcac ttgcaagtga atgggctgaa agacagcgac aagatggaaa gaaagtacaa
     4081 gtatacaaat tatcaatcgg acaattgcgt gttgggggag atgatgtcct ccaaacacgt
     4141 ttagaaacat taattccaag attgaaagaa tatgagcgaa ttttaaaatc tgaggacaaa
     4201 gaaaatgaat ttgtcctctt cattgacgag tttcataaag ttgttagctc ttttggtagt
     4261 ggcaataaaa ttgggggtga ttcgctaaaa gaaccgttag caaatgaatc acttcaattg
     4321 attaccgcta ctacaagaaa agaatatgaa aactatatca aaagagatga tgccttaggg
     4381 agacggttac aaccgttaga attgaatgaa ctaaaaagcg atgttgtcgt cgatattttg
     4441 agacaatggc taaatacatc ctctgttaaa gatgatcctc aggcttcttt taaattatcg
     4501 caaaggattc ctgatgaatt acttcaagaa atcgtacaag caaacaagct ttatcgtgag
     4561 gatttgtccg aaccggctaa atcaattgcc gtaatggagg atttgattgc aaaagatcga
     4621 gtagacggaa atgcttataa tcgagaaagt ctattgcaaa tatttggtaa gtataatatt
     4681 cagctagatt ctgattttga tatggaaaaa cttcaagagg aattaaagtc cataaaaggg
     4741 caaccgttag cagtttatac tataacaatg ttgtttaaac gtatgaagct tggatttgat
     4801 cgttttaaag gtcgaaagca accaaaagca actgctctgt ttacaggatc aacaggggtc
     4861 ggtaaaactg caatggctaa agccgtggca agaggacttt atggcgatga aaataaagtt
     4921 ctcttagtta atatgccgga ctacaaaaca aaagatgatg caatgcgttt tagacgactc
     4981 ataggtactc atgtaaggca tgatccttct gcgattattg tgtgtgatga atatgagaaa
     5041 gctggtgtag aaacactcga tgccttttta tcaattttag atgaaggtag gataagtttt
     5101 actgaaagag gatgggatga tcgagaagtt cacgacactg tttcattgtc aaatacaatt
     5161 ttatttgcga caacaaatgc aggtgcggag atgttcgatt cgttaaacag atatagctca
     5221 ctatctcgtg ttacaaacaa ggaaaattct aatgaatata gcactgaact aaaaagagaa
     5281 taccgaaaag tcgcatcagg gatcaaacaa gccttgattg gtgaaggtct aaagcctgaa
     5341 gttattaacc gatttggacg aatagttcca ttttgggctt tatccgaaca aactcttctg
     5401 gaaattgctt ataatgaaat gttgaagagt gctgaattag tacgaaaaca tttaaatata
     5461 gatttacgtc ttccacctag gaaagaaaga gtatatggtc aaaattttta tgcagacgat
     5521 ctctccatgc acttagtttt tgacatggct tcacaagagg attctaaatc agggggtgca
     5581 aggaatatct tgcatttagt tgaatctgag tttgaagaag cgattactga agcaataatt
     5641 acttatccaa acgcaaaaat attcgatgtt aagttgagtg aagccggagc gtttgaagga
     5701 aaaggtgatt ctaatgctga aattattgca aagccttccg ttgctgaaag taaagaaaac
     5761 aagattgtat tttcgattgg aggggcgtag ctatgaaatt caagaaatat ttactacaaa
     5821 accttagaat aaaacgatgg gtcgtaagag gtctagttat tggaggagta ttagcagtta
     5881 ctttaggcgc tccttttgga atgaatgatc caactcctga caatcaagat agcaaacaag
     5941 aaacagttat aaataagaac gttgcatatg cggaaagtaa accggcagaa caaggaatgt
     6001 ggacactata tgctcgtatc ctaatgggag ataaagaagt taatgatagt gccgctaaaa
     6061 ctggacaaat gaatttgtta ggacaaggtg gtatcagtgc cgatttccct tattctgaca
     6121 tttctaaaca aggtaattcg attgcagggg gtaatgcagg tactcaacta gcatctactt
     6181 tagcaactta tagttactac ggatatattg aaacagtctc aggaaatgct attgccagtc
     6241 aagcatcatc cattctagga agtattggta ggacgattgg tagttttatc ggatcaatta
     6301 gtttaatcct ttctaatatt gctgaatcca taaatgaact ccttggaaaa tttttcatga
     6361 gtatgaatat attttctctt atggggtggg gagaaggtgg atcggatgta tctaatccgg
     6421 tatctaaagc attaactagc ttgcttaaca atttgggatt aaataatgaa ttatttaaag
     6481 cgatagcaag cttatctatg ctctttttca cagcaatact aggttatcaa ggaatgaaat
     6541 tattgtcgaa agatggtgtt aactcgaagg gattatcacc agtacttgaa aaatggattg
     6601 ttcgtatttt tgtattgttt atattcttac cagtggcagg atcgctattg ggagtttata
     6661 tcaacggcat ccagtcagca aaaaaagatg gagatatacc aacaaatgtt gtttctgact
     6721 acatcattaa cacaagactt tgggcttcta ctcaaaatct tgctccatca tctgatactg
     6781 gtggtgatgt acctgacgga aagcctacat ccgaaatcaa gttcgttgat acagattatg
     6841 aaccatctaa aaaatctgca atgataagag cgatcaatac tagcagttat gccagacaaa
     6901 ataatgatct agattctaat tcagtcggta tggctctatt aaaagaatgg tcatcgaatg
     6961 atactttcaa tattaatact tatgccggtg atgttaatgt cgccgaaaat gatgcttata
     7021 tgggaagagc gcctgctgtt tggacaggct ataatttagg ggatggaaaa gaaggaagtg
     7081 caattaagaa accaaccaat ttaaatggat atatttggtc agcaaatcaa aatgtgacag
     7141 acgacaatcg caagcctgat ggaaaaggaa cagcgcataa ctttgatgct actcaacctt
     7201 ggggcgttgc taacaatgca acttttagca cacagtcggt agctttattg ctccaatcgg
     7261 cacttgatac atcgggagct aagttttatg cgtataatat cgctccttca ggaattcaag
     7321 gcagtgctaa gaatatgtca acagtaaaaa cagaatggcg tacagcatcc ttagttggtc
     7381 atggagctat gggtaagttt ggctcgtggc tagcgatgaa cgctgaatca atcgttttag
     7441 cagttcttta cttagcatgt gcttgggcga tgttcaatgt gaatatcgtg aaagcattta
     7501 aacgtgaatt tattcggatt tgggaagtag ttctaacagg atcacttgca tcaggagcag
     7561 gtgtgatggc aatttcatta ggtgttgtta caacatctct gatcggacta caattaccta
     7621 tctttctgat tacttttatt cgtgcatttg gtgatactgc tgtgggattc attcctaata
     7681 cattagttgg cgatggcgta gcaaatattg ttcgatctgc tatgatttta ggtgtaggat
     7741 atcttttagt ctttaaaaaa ggcgaaaaag taaataattt gattaatatg cttattggct
     7801 taccacttga agtagcgttt gggtttgatg aacgtgctaa gttattattt ggtaatagaa
     7861 atgatgctgt gcgagttgtg gacggcgttt ctaaagcaat caagaaaagc caaggagcta
     7921 aaaatcccta ttatgatggt ggcaacagct cagtaaaaga tatggttgca ggaggtcttg
     7981 gagcagttgg agcgcaaaaa atggctgaac gtttgaacaa tatgggagat agcaatgctg
     8041 atgctaagtc cattgctgat aaagtaattc ctgaaacacc agaaggaatg tcattcaacg
     8101 gtggcggagt tggttccaca atcaataata cctctttcaa agatttagtt cgtcaagatc
     8161 aactacgtaa taactcccaa caatatattg atcatttagg acgtatggtt gatgaaaacg
     8221 gaaactttgt cgatgaaaat ggtgaaatat taaaagatag taaatacggt aaagatgcta
     8281 aaggcaactt tattgacgaa aaagggcgtt tggtcgatga agatggaaac cttatagata
     8341 ctaatggtaa accattggaa gatagtgaag atggtaatat tggtatggat agcgaaggac
     8401 gtttaattga tgaaaaaggt cgcttagtcg atgaagatgg aaatcttgtt gacgaaaatg
     8461 gacgcaggtt gctaaatggt gattttggtc atgatagtaa cggacgttta gttgacgaaa
     8521 aaggtcgttt agttgatgaa gatggaaatc ttgttgatga gtttggtaat aaacttggta
     8581 aacacagcga tggatataat gtgaatggag atttagtcga tgaggatggg aatatcattg
     8641 agacgagaga tgcgcttgtg gcagatcgaa atagagaact cgaacaacaa cggcatacgc
     8701 caattgctgt tgcaattcct gagaatgaag aagacgagat tactttagat caaactcatc
     8761 aagaactgag gaatgaaatg agtggtgagg acgtgtcaga cagagtgcat cgggaattag
     8821 gagatggagc atctggtgaa agtcagtcaa gagagcttaa cagcaacttt gataatagtg
     8881 ctattcggaa tgacacagta cttaaagaca gagaatcgct aacaaaagaa acggataaag
     8941 aacaatcaga tgtgaagaaa aagttcattc ctactcctaa tgttaatcaa acagataaat
     9001 atgaaacaaa tattggatca ggaatgtcga aagacaatca atcaattaga gaacctgaac
     9061 tgacaaataa aaataataga acaaataatt ctaaatctac ttcagataat tcagggaaat
     9121 ctaatcaatc aaccaagaaa acatcaacag ctaaatcagc cgttgaatat ctatcaaaaa
     9181 ctggtaaaaa tgtttacacc tcagcagata aagctttagc aaaagctcca aaaggtagtc
     9241 tagcagggat aacctatgaa gctaaaaacg aagctattaa aataggtaaa ggaacggcta
     9301 aggttgccaa ggctactgct acaggagcat ccaaaactgg taaagtagtg aagaaatcta
     9361 ccatgtcagt caaggactat attgtaaaat acggtaatca aaaatcaact gaaaataagt
     9421 ctgaagacga gtaaaaagaa ggagcattga tttgctcctt ctttttactt tgatttctaa
     9481 ctgaagatgt caaggaaaat ttctatttat gcaaaaaaat tattttaaat tgtctataaa
     9541 tgaattaaat atttactgtt caaagttgta taattaaaaa gggcttatgc tggtaaagaa
     9601 cagaattatt atctaaacaa aaacttg
//
LOCUS       DAZUKA010000106         9622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000106 DAZUKA010000000
VERSION     DAZUKA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.109"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..803)
                     /locus_tag="ACG8PN_002111"
     CDS             complement(189..803)
                     /locus_tag="ACG8PN_002111"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4862246.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(950..1633)
                     /locus_tag="ACG8PN_002112"
     CDS             complement(950..1633)
                     /locus_tag="ACG8PN_002112"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4862247.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1742..2200)
                     /locus_tag="ACG8PN_002113"
     CDS             complement(1742..2200)
                     /locus_tag="ACG8PN_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4862248.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2284..4047)
                     /locus_tag="ACG8PN_002114"
     CDS             complement(2284..4047)
                     /locus_tag="ACG8PN_002114"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4862249.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4268..5728)
                     /locus_tag="ACG8PN_002115"
     CDS             complement(4268..5728)
                     /locus_tag="ACG8PN_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862250.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5936..6862)
                     /gene="glsA"
                     /locus_tag="ACG8PN_002116"
     CDS             complement(5936..6862)
                     /gene="glsA"
                     /locus_tag="ACG8PN_002116"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4862251.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6885..8312)
                     /locus_tag="ACG8PN_002117"
     CDS             complement(6885..8312)
                     /locus_tag="ACG8PN_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4862252.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8343..9557)
                     /locus_tag="ACG8PN_002118"
     CDS             complement(8343..9557)
                     /locus_tag="ACG8PN_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4862253.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2842 a         2016 c         1605 g         3159 t
ORIGIN      
        1 gtttgatctg gcatttctat ttccttacca tctaaagtaa cagtagtaac gacaaaatgg
       61 tcttgcaatg gcggaacttc taagtactgc ccttgcagaa aatcattcac tgtttcatat
      121 tcttttttca tttcttttga atctgtcgat ggtttatagc ttatttttaa cataactagt
      181 gctccttttt attcgggacg tgcaatcgtg aaaatttctc ctattgctgc ataatcattt
      241 cctgccaaac ggcttacagc attcaatttt cttggatcga tccttcctgt cttatcatca
      301 tagactgttt catttaaatg atatttctgt acttctagca aaaataaatc agctgttact
      361 ttatttttat tccgtatctc gacatgttca tataaggatg tttcaaaacg aacttttgct
      421 tctgtcaatg ctggtactga aatgattact gaatcagtta aagtcaagtc agtcaggtac
      481 agttcgcttt cctcattagg cagattggct gctgttttat ttacttcttc tacgttgtcc
      541 tcatcaagga tatggataac agcttctctt gtctcaagaa tattctttgc tgtgtctttt
      601 actgcatctt gttttctctg gatcgccaag gataagatgg gtcgattcga actgacgata
      661 ttgaagtaac tgaagggtgc gatattcact atatcttttg ctgatttagt cgttactact
      721 gctactggtc tagggatgac tgaaccaatc agcaacttgt aattttcacg ttctgtcaat
      781 tctgaaggac tccgttcaaa catggtttcg ccttctttct ataaaattcg ttaaaaagca
      841 gatcttgaag acaaaatgaa aagtaatgct tttttcatct tcttagaatc tgctttaaac
      901 tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg
      961 ctttacttgc ggcttcgaca tatcttttag tgatcaattg cggacgggtg ataatagatc
     1021 caactacgac tgaaaaagca cctaattcat atgctttctc tacttgttca ggagagtcag
     1081 tatgtccttc cataattact gggatatcga cttgttctat gatttgattc aataaatcaa
     1141 aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa
     1201 gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa agtcgaaata tctgccatag
     1261 ctaaccgacc gtgttcgtga atcatttgga caagttcagc agttgttttt tctgtcggac
     1321 gtttacgaga agttgcatcc agtgcaatga tttctgcttg cgtctccaaa agttcttcca
     1381 cttctttttc tgtcgcagta ataaacactt ctgacccatc gtaatctctt ttgataatcc
     1441 ctattaccgg taaatcaacg attcgcttga tttcattgat atcttctgtc gtatttgctc
     1501 gaataccaac tgctccgcct tcttttgcag ccaaagccat tctccccata ataaaagaac
     1561 tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac gactccgcct ttgatttgtt
     1621 ccagtgtatc cacaatatct cctccagtaa tgttatataa aaatacagct tcatataaaa
     1681 atgtaaccct tttctattca ggagacaata atgagctatt tctttcagca gacctttctt
     1741 actaaaagaa aattacttca aattcttcgt atacgaccag cttctcttct gtaaattcta
     1801 tgttcaacgc ttctttataa gcatgtttaa aaaactcagc atggactttc cgccaataag
     1861 ctaatgtccg atccccttct ccttctttcc aagcatgctc tttcgtgact tcactaaaag
     1921 gcaagatggt cacatttata tttttgatga tacaaactgg ttcatttttt ccattcagga
     1981 ccacgtctaa ttgaccaact tttggtaaag gctcattctc ttgtttatac agatcgtaac
     2041 cacttgatgt accagtcttt atctttttta caactagttc gctcaactcg tctgccattt
     2101 tttctgtatc cccaaatgaa aagaaaccta atttttctga ttgataacta ggatattttt
     2161 cttggaactt tttgacatat tcttctactt gctgatacat aacatccacc cctttgcttt
     2221 ttataatgaa aaaaccatcc agatactagt ataaacaatt atctggatgg tgagcataat
     2281 gaattattga atattcaaga cacgcagact atcttcaacg atttgttttg gtgttaaatg
     2341 ataacgttca taaagctgcc ctacaggaac gctatccgtg aattctttct ctgcaccaaa
     2401 atttaatacg ttcattgaag aatcaccata ataacgactg atattttcgc caaatcctcc
     2461 tgacaaacta ccatcttcta atgtgatgac taattcgtgt gtttctttta atttatctaa
     2521 tgtttctacg tcattttctg tgatcgttcc tggattgatc aacgttgctt cgattccttt
     2581 ttttctaaaa gcttttactg tttcttttcc taattcaaga aaaccgccta aagctaaaat
     2641 tgctactttt cctccattat ttactgtttg ataggctgga atactataat catttgcgat
     2701 agctgtacca tgttctacac catgtgtagg aattcggatt gctacaggct gcgctgtttg
     2761 ttcgaacgcc caatctagca tagagatcat atcctctttt gttgctggag caagatattt
     2821 gatgttcggg atacttgata agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact
     2881 agctgagatc gttccgccac cgactaaaat aatcactgga ttctcgttga tcgctaaatc
     2941 atgtgataat tgatcatatg cccgttgcaa gaaagtactt gattggaaga cgactggacg
     3001 tactccctga gaagccaaag cagaagcgaa agtgatagag tgctgttcag cgatccccac
     3061 gtcaaaataa tgatctggat gtttttcttg gaatgttttc agtccaaagg cgcctggtat
     3121 cgctgcattg actgcggcaa taggagctgt acttgtttgc attttcttat ccaaataatc
     3181 catgatgatc atattaaatg tttctccaga agttacttgc tttgcctccc cggtggacag
     3241 atcaaatggt acatgccaat gatatttcat tttattatcg attgccggct gataaccgcg
     3301 acctttttcc gtatggacgt gaagtacaat cggatgatca atatctttta cttcttcaaa
     3361 taaacggatc accgtttcca gatcgtttcc attatccacg taacgataat caaatcccat
     3421 tgctttaaat aggttattgc tgcttttacc gttggtttga cgaagctcag ctaagttttt
     3481 atataaactg ccatgattct catcaataga catctcatta tcgtttacta atacgattag
     3541 attggattgt aaaacaccac cattgttcaa tccttcaaaa gctaagccac cacttaggga
     3601 gccgtcaccg ataaatgcta ccacatttcc tgattctttc ttcagatctc tcgctttagc
     3661 taaacctgtt gctaagctga tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt
     3721 actttcatgt tggttggaat atccgctcac ttcacggtaa tgtccttttt caaaaccatt
     3781 ttttcgacca gttaaaagct tatgaccata agactgatgg gaaacatccc aaacgatttt
     3841 atcctttggt gaatcaaata cataatgaaa cgcaatcgtt aattctacta cacctagatt
     3901 cggaccaacg tgcccaccta catcgctgtc cttttgcaag attttttggc gcatctcttc
     3961 tgccaactgt ttcatttcct caacagataa ttttttgaca tctgcggggc tattgattgc
     4021 atccaataca tgtaatactg tactcataat acattctctc ctccgtacta tcttctttta
     4081 atgaaaatag atttgttaca ctttatctat tctactactt agagttgact ctaagtcaat
     4141 agcattttaa aaaaatttca gagaaagctt gttatataag tagatcttcg cacgaatcag
     4201 gactcaaata aaaaccttct acagacaatt aaaaagcttt gtagaaggtc tcctaattaa
     4261 ggtctcatca agaaatatgt ttttttaata ggtgaagttc ctgagcaata tgatacaaat
     4321 ggatgtttgc accttttaat gagattggat agatctttgt cttttcagtt tctaatccat
     4381 tttgatagct agcagcatcg cacaaaacca ctcttttttt cgcacataga cattcatcag
     4441 gcaattcttc taatgaacga aataaaatag gatttatttc agcgatgcct tcttccgcat
     4501 acaaatgaaa ttgggttcga taaaaaaatt gtttgactga actttctgaa acaacaaaaa
     4561 tttctatttc ctgtctcccc gcttgttcac tgttgatcaa agaaagactt ttgacgaatt
     4621 ttcgaatgaa attttcattc catcttagtt ggatttgatt tttctttttc cactcttcca
     4681 atatttctac aatttttccg tacagaagtt gttctttttc tgatagaaga tagatccttt
     4741 ccggtaaaaa tacttgtcca tctgcccaca acgtacggac aaattgtaaa aaggattgct
     4801 tgaaaataaa attttctgca tcgtcgattt cagcgatact aataatctgt aaaatcaatt
     4861 cattcagtga taggtcattg agaacaaaat ttttataaac aacttcgaaa tcttttctta
     4921 acagctccat gttctcattg gtataatttc tactgttgaa caaagaaaaa atataataga
     4981 catcttcttc ttgtaagtct agaattcctt tttgctcttc caatccttga cagactaatt
     5041 gaaataacgg cttcgtttct gcgcttttct tatcttcttc tttcagtcca aggggaaatg
     5101 cctttcttcc gattgcgatc tctattcctc gtatgataaa ggacttttca tcttccgaat
     5161 atcttctttt aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa gcttcttcta
     5221 catacggatt tttattttca tagccttccc aattgacata acgtactaaa gctagcaaga
     5281 tgttccgcga gtcaaattct gggataatga tttctttatt tttcaaataa tcattttctt
     5341 taaaaaaatc caatacgatt tttttgattg tatatacttt gctcaaagaa ataaattcaa
     5401 gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa
     5461 aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata
     5521 cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg
     5581 ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt
     5641 gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata
     5701 tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat
     5761 tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt
     5821 caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc
     5881 attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt
     5941 caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc
     6001 tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag
     6061 ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact
     6121 ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt
     6181 cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag
     6241 ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga
     6301 gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc
     6361 gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag
     6421 gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt
     6481 taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat
     6541 gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg
     6601 tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg
     6661 gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt
     6721 gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg
     6781 ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt
     6841 gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc
     6901 tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa
     6961 acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca
     7021 aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt
     7081 acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc
     7141 ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac
     7201 atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag
     7261 attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct
     7321 tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga
     7381 gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt
     7441 ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc
     7501 acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca
     7561 aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt
     7621 ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa
     7681 ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc
     7741 atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac
     7801 aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg
     7861 tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc
     7921 aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca
     7981 gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt
     8041 gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt
     8101 tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc
     8161 aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat
     8221 tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt
     8281 tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg
     8341 tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt
     8401 gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag
     8461 gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt
     8521 ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat
     8581 ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga
     8641 aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt
     8701 gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt
     8761 acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc
     8821 gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga
     8881 agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca
     8941 gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt
     9001 gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg
     9061 aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg
     9121 aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc
     9181 cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga
     9241 aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt
     9301 ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc
     9361 aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga
     9421 gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca
     9481 ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg
     9541 ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg
     9601 tagttgtttt tttcttctgt tt
//
LOCUS       DAZUKA010000107         9589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000107 DAZUKA010000000
VERSION     DAZUKA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.110"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..294)
                     /locus_tag="ACG8PN_002119"
     CDS             complement(100..294)
                     /locus_tag="ACG8PN_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862254.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(307..489)
                     /locus_tag="ACG8PN_002120"
     CDS             complement(307..489)
                     /locus_tag="ACG8PN_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862255.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(486..626)
                     /locus_tag="ACG8PN_002121"
     CDS             complement(486..626)
                     /locus_tag="ACG8PN_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862256.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(1116..1481)
                     /locus_tag="ACG8PN_002122"
     CDS             complement(1116..1481)
                     /locus_tag="ACG8PN_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862257.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(1521..1823)
                     /locus_tag="ACG8PN_002123"
     CDS             complement(1521..1823)
                     /locus_tag="ACG8PN_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4862258.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1823..2122)
                     /locus_tag="ACG8PN_002124"
     CDS             complement(1823..2122)
                     /locus_tag="ACG8PN_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862259.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(2115..2279)
                     /locus_tag="ACG8PN_002125"
     CDS             complement(2115..2279)
                     /locus_tag="ACG8PN_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862260.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2285..2554)
                     /locus_tag="ACG8PN_002126"
     CDS             complement(2285..2554)
                     /locus_tag="ACG8PN_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862261.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(2566..3315)
                     /locus_tag="ACG8PN_002127"
     CDS             complement(2566..3315)
                     /locus_tag="ACG8PN_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862262.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3312..4004)
                     /locus_tag="ACG8PN_002128"
     CDS             complement(3312..4004)
                     /locus_tag="ACG8PN_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4862263.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(4010..4681)
                     /locus_tag="ACG8PN_002129"
     CDS             complement(4010..4681)
                     /locus_tag="ACG8PN_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4862264.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4674..5015)
                     /locus_tag="ACG8PN_002130"
     CDS             complement(4674..5015)
                     /locus_tag="ACG8PN_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862265.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5193..5891)
                     /locus_tag="ACG8PN_002131"
     CDS             complement(5193..5891)
                     /locus_tag="ACG8PN_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4862266.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            5978..6448
                     /locus_tag="ACG8PN_002132"
     CDS             5978..6448
                     /locus_tag="ACG8PN_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862267.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6491..6670)
                     /locus_tag="ACG8PN_002133"
     CDS             complement(6491..6670)
                     /locus_tag="ACG8PN_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862268.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6657..6995)
                     /locus_tag="ACG8PN_002134"
     CDS             complement(6657..6995)
                     /locus_tag="ACG8PN_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4862269.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7011..7214)
                     /locus_tag="ACG8PN_002135"
     CDS             complement(7011..7214)
                     /locus_tag="ACG8PN_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862270.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7229..8005)
                     /locus_tag="ACG8PN_002136"
     CDS             complement(7229..8005)
                     /locus_tag="ACG8PN_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4862271.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8304..8624
                     /locus_tag="ACG8PN_002137"
     CDS             8304..8624
                     /locus_tag="ACG8PN_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4862272.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8642..9073
                     /locus_tag="ACG8PN_002138"
     CDS             8642..9073
                     /locus_tag="ACG8PN_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4862273.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            9203..>9589
                     /locus_tag="ACG8PN_002139"
     CDS             9203..>9589
                     /locus_tag="ACG8PN_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS4862274.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKY"
BASE COUNT         2748 a         1882 c         1492 g         3467 t
ORIGIN      
        1 tgattattgg acgcaaactc gtgagaatga attaaattta gaaaaactca ttattaaaaa
       61 gaaatagcaa ctccagccca cacggctggt tttttagttt caacttccct ccttacgggt
      121 caaaatcatc ggttccggca taatctgatc atcctttagc aacaaatcag gatatttttt
      181 ccttagcttc ttattgttcg tgtaagtttt caatagccat tggttggctt cgcttttgct
      241 ttctccagaa aaataaattt tccctgtttg gatgcctgca agttgaatca tcattttcac
      301 ctcaaatcaa attttttctg ctagatggag caaaagcaat ccgacatcaa gaatcttttg
      361 acccattatt tcagctactt tgctcggctt aactccttga gaaaagagca agcgtgcttg
      421 ctctatctct tcgtgagtcc aaataaattt ggcttcctca aggataatta ctggatcagt
      481 tgctctcatt gttcctctgg aaaaatgatg tcattcttat gcttggacca ggtatctgca
      541 tatggaacaa agtattgtct agctaattcg atttgatcaa ttaaagcagt ttctgataat
      601 tcatggtctg cagcaatttc attcatacta tcgccttcat ctaaacagat aagaccgttg
      661 acgttatcga tttttacatt tgtgactgct tgactttctc cacgctcata atattctaca
      721 gttgatactg atacatcagc ttcggagttt aattgcagaa tctgttactg ccgggataat
      781 ttaatattct tttcataatt cacctttttt cctactgaca tcgaatattt ttcatgttat
      841 gattagccgt gaaaggagag ctatattatg tgtttacaca tgatgtcctt agctcattcg
      901 gttctttgga agcttttttg gaaataactc tcaggttctt gacgcaaatc tcatttattc
      961 tttgaaacaa aaaacttcca ttttccgaga gctttggatc acttcaaaag agatttgcat
     1021 ttatatttct gtgaccttga gtcgcgtaaa gaaccttaca gcaatggcgg caattccact
     1081 ttcaggaaac cgcctttttt cattgatatt ggtggttagc ggactaccca ttttctttcg
     1141 gcagtgccaa accacaaatg atctgtgttg atgtttaggt agttgtatac cagataagct
     1201 aaatccaaag tgcctgcatc ttcaagacga caatattcca aaaattctcc catagctgga
     1261 cataaaaatc ctggagctat catatcccaa cagttaatag tgcaaagaaa ttcgtttagc
     1321 ttctgaacgt tttctttggt ttcacgaatt acaacatact ccaattcttc tccgctggca
     1381 gagcattcca aaacttcaaa ttgattaaga aatggtagaa catatgcttc aaacatcaac
     1441 attccatctt tctgtttttg cacttgtttt gcatcaatca tgtctatcct cctattcagt
     1501 tatttcttcc attaatggaa ttatttcagg tcactggact taacgaaaac accattgacc
     1561 atctttccag tccgaccttt gatttcgtca taagcaaaat ccaagcactc gtataaatcc
     1621 atatcatttt gcattgccaa gatgatcaag gttactacta catcgccaat tccgtctctt
     1681 aaaccatgtt catcttttct agctagagaa gcggcaactt ccccgatttc ttcgaccgtt
     1741 tttaacattt gcttgctgaa atccgcttga tccaatccct tatcttttgc ccactgctct
     1801 actttcgcga ttagttcgtc cattatttct cctccacata cctaaactgt cttccttttg
     1861 aatcaatcca taagctccta gctctatccc aaataatatt tttgctcaga ccagtaattt
     1921 cagataactg tttagcggta cccgttacta gaattcgatc accatgccag attgcaatct
     1981 ttctcggcgt tctccgtttg ggcttttcag tccacattga tttaccgagc ttttggactt
     2041 ctgcaactat ttttttatct tcctgccaat cttctgaata agtcaattcg ataattcgct
     2101 gcattgccgc tttcttatcc atcccgacat tctcctttca gtaatttgag tacttgatca
     2161 agtgcgctct cacgtccgcc atgaaatgtg ttgagccact tgtcttcgta cgacacactt
     2221 tgtcttaaag cttcttgatg cattaattcg atctgtgctg taaatgtctt taggtccatc
     2281 ttgtttacac ctgctcaagt tcattaagat gtttttgcaa gcctttaaca caatcaatga
     2341 atagtaattt ggtatatgct aaatttctta attgcgttcc atcgatatag agtgcgaaat
     2401 agtatctgag tttactccaa ctcgaacgat cattcttaat tccttcaatt ccagcttctt
     2461 cgagttgatc atatacgtct ctcaggacgt ctaattcctc gcccttttta taattcgcta
     2521 tttcattaat tagttctaga taatcgatct tcatttttcc acctcttaga atggtgcttt
     2581 tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga taggcttctt ggtcgattgc
     2641 ccattcagga agcttctctc gtcttcctgt gcgcttgtat ccactgcttg cgttcttagg
     2701 ttcacttttt tctttccttg cccaacttcg aatagttgcc aaatagtttt tataagtctt
     2761 accagatgat tcacaatact ctgacagtcg ttcgattcgc tcttggtaat cattagggaa
     2821 ttctgttttg agtttctcca tctgctcatc tgacaaaaga acatttttat actctccgta
     2881 tttatgacgg acgggcttag ccttcgattt tttcgaaggc ggtaactctc ttatatattc
     2941 ttttgtatta ttaaatgtat tattaataga tgtattatta tctttgactt tttcgtcaat
     3001 aggggtattg cgtttttcgt caatagggta ttgattaatt cgtaggtacc tattgattat
     3061 ttgattggta ccctctttgt aaatgatttc ccgattcaag tatccaaact taatcaaatc
     3121 acttacccat cgcgatatgg tctctttatt cacaccatat aaatctgcaa agtactcatt
     3181 gcctgcccaa caaaagcctc tttcattaca caaggccgtt atctctccgt ataacaactt
     3241 agtatttggt ttaagtcttt tgtcgtacct tacgttggct ggtataatcg cataataact
     3301 tcgatgttct gtcatttttc ccctccaata tttaactttt tgattgtttt ctggtttaat
     3361 ttgatccctt tgatttgata tttatttttg aaattaatca cacctatttt gtgcttctct
     3421 gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg catgatcaac ttctttgcgc
     3481 tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag ctcctgtttt gccacagatg
     3541 cagcagactc tttttgtgat gcatttgtag aagtaatatt cttgattcgc aggtaaaatc
     3601 tcatagcctt ctttgaaagg aatatgatgt tcaaagatga aatctaagat gatatttgct
     3661 aagacattgg catcactcac agttgtattc gattcgtctt tcaggcttat tttgcgccct
     3721 gtgacacctt caaaacgaaa gtagaagaat tccttccaga agtccgttgg catgcctgta
     3781 tcgataaaaa tatcgcctat cagcgcatag atgaagtttc gttgctgtac ggtgaaacgt
     3841 ctaggatcaa taaaacgaat ttcaatgact cgatcaccat cgtagccgtc atacatcgtc
     3901 tttagtcgat caatgttcac ttcctcattg atggttgcgc ttatgtcttt tcctttgaac
     3961 tttttcagaa ccgctgaata tgaatcgatt aatggtttaa acactcatat cacttcttat
     4021 ctaattcttt tctcttagct gctattgctc gctccatcaa ggcacattgc tcatagctta
     4081 actgttcaat agtttcaacg ttatcagcta agagccctaa tttatctgtc tgctcattaa
     4141 catattcgat taaggttttg gtcatatctt tacccatctg ctcattgaaa gcttctagaa
     4201 tcgtctctag catgtttaat ttctttgtat cgattctagg tggtgttgga atatcttccc
     4261 cttgaaatac atataatccc agtccgtgta gagccaatgc tttcacaaag catcgcttca
     4321 atgagttatt gatttgcatt gcatttggtt taacaactgg ttggtttcga taatctaaaa
     4381 caggaaataa ttcggtttcc gtgtgtcctt taaccgttac tgagacagat acataagtcc
     4441 cagtttcatc cataagaaaa ggtttatatt cctcaacaag aaagtcttga tgagttccag
     4501 aaacaaccct gtagtgtttg tactcattaa tagttaccgt tgcctgtgga tcattctttt
     4561 tcataatctc ccacgcgtga gcccaagata aataatcaaa atttcctttt ttcttgagaa
     4621 ttttatttaa cttacgacta aaaagttttt caaagttcgt tgtccctttg atttcactca
     4681 tcaaattctg cctccatttc agcaatgtat ttcttacctg gtccgtaata agagatatca
     4741 atcaagttat ctctgtcgta ctcttctagc gcatcaatca agccatcttc gatgacgtaa
     4801 atatattcag gtttattcga atgcttcgat agatggataa gataaacatg atcccaaata
     4861 gttacatggt tgcctaaatc atcttgatcc caagctagtt cttcattcgt caaaagattt
     4921 cgtctgattt ttcgaccact tgtttcctca attttcggct tgccccaatc aggatcaatc
     4981 aaatattgat ctagagtgga aagttctttt tccatatgct aaaatctcct tatgatgtgt
     5041 tttctttgtg actctttgct tgccggcgga gtcacttttt tatttgttgc catgcttttt
     5101 gcttgtcaat atgttgttgg cttaggatga ttggtttata gtatttccac cagcaattag
     5161 caattgccgt ccctattctt agcgcttcag ctctattcat tgtcatcacc aaaaagtctc
     5221 tgttgtctgt tcagttgatc aatttccata cggatcgcag tttctggcaa ccacatttca
     5281 ataaatgaaa cagcatcatc gaatctctta cgaggtaact cgccatatct tgggattgaa
     5341 aaggtacgtt taaattcaga ccaaaatttt gagaatactt ttttgctgat ttcttcataa
     5401 gctcggcttt cttttccccc tagaactccc ataactttca tatttccttt ttgcttaatt
     5461 tcaaactctt gttgtccgct aattcgcata gtatctttaa gcatggaaac atctttttta
     5521 acatctttca tttcttctaa ttgatagatc atcatatctt caattgtttg aggaacagta
     5581 ttcttgcgaa taacatcttc catttcgttg aatgcttcaa tgtatttttg tttgaagtag
     5641 atagctttct ttcctgtaaa ccccatagcc agcaagaaaa atccatctct actaatgaag
     5701 aaaactcgtc gatttctgcc atatgaatct ggttcattac cttctacaaa catctgtcca
     5761 aaattggaca catctttttt tagtgcatca atatctctta aaacatgttg atgttttttc
     5821 tcgaagcttt ctgccacttg taagctcgta gtcacagctt ctttattttt caaaattact
     5881 aattcttgca ttatttcttc tctccttttt ggtataattt agttaaaaac tggtggtgtt
     5941 tattttgaat ttttttcact taacaatttt tcaatggatg tcaataataa gttttatatt
     6001 gagtacgttt ttaacaggga taaaaataaa aggaatgcgc cctcaactag aagttgaact
     6061 taatgcttct tacttcgctg cagatatgat ttatacaaaa gtaatcattt ctaactattc
     6121 aacagaacca gctatgttag ttaatttaga actttcatcc gctaacttaa accgcagatg
     6181 gtcagctact ccatttaaga aattaattgc taaaggagga tcagtcgatg ataatagaat
     6241 atactctgaa gctgtaccgc taaatattcc tcctaaaagt gctatatctt gttaccttgc
     6301 atttgaagta gggaaaatta attttaataa actccttaat caccaaacaa aaatgatatt
     6361 tactctaaat agaacccaaa tccacaaaat tgttgatatc aaaaatacaa acttcccaat
     6421 agagaaatta gtgaaagaat taaactgagt aatagtaata tacattctag tttctctttt
     6481 ctattcattt ttagtcagcc ccctcggttg gctttttcgc tctgtactca gcttcagctt
     6541 catcaagccc cataaaaatc caaaccatgt aaacaatcgt tcctatcaac gcttgcctgc
     6601 ttccccaaag tcctaaagcg tagatgatta gcggtgcgct gaacactagc gctctattaa
     6661 attttcccat ccgcttgcct ccttaagatt tccgaaaaat ttgtttctaa aaattcaagt
     6721 gttttacttc ttaaaaatag atatgtgtct cttccttcaa ctggataata gacaaatcca
     6781 tttttgtttt tctcgatatc gataatattt ctatatcttg ggttctttaa aactctagaa
     6841 gtaaaccaat catattttct gttaatccgt tctagcactt ctggcaacgt catccatcta
     6901 ccagtatcat cagctttttt taactcctca taatccactt gggagataat tacatagcct
     6961 tctggaattg ggatttttgc ttctagatat tgcattagct gttcctccct ttacaattcg
     7021 tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt tttaccgttt
     7081 aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt
     7141 aagaactttg aactttcgac atattctttg attttttctc tatctcgttg agtgatttct
     7201 gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc atttgagggt
     7261 aatatccttc tttttttagt aattcgtaga taaatagacg ccctttctgt gtccatttgg
     7321 tattcattac aattttagtg ccaccatcgg ctttcgggat ctcagttgta tgagattttg
     7381 tgtatccttg gttcatgtgt tttttgcaca ataaccattg gttaccgact tttttctgaa
     7441 tacctagttt atgaagtaat ttattcatct gttgtggaga catcccataa tctgctgcaa
     7501 tctgactaat tgttactgaa tctgtagaag ataatatgct atctaaatag gagattttag
     7561 gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc agttctttct tcttttagtt
     7621 gagttgctaa ttgaatgatt gtatctggat taagcaaagc ttcttctact ttttctggag
     7681 ttagataacc tccatgtttt ctaattgctg gcaacacttc acttgttacc caacgtttaa
     7741 attttttggc agagggaagt tttgatttta agattaaact gtacaaacca gattcgttga
     7801 tgattgtcat ctctcttgat tgacctgagg tcgtgattcg cgaccccatc ttatcttcca
     7861 aatctacatg ccgcgacaaa gcatctttag tgtttgaata acccaaaaca ctcgcaacgt
     7921 ctttgcctac aaaatatggt tcatcattta ctaaaattgt ccgaacttcg ttttgttcga
     7981 aattaaaaat ttgcggtgtg ttcatatttc tcattccttt cttttatatt cgtaaacaaa
     8041 tttaacaact tttttctaaa ttcaattgac agtatctgag taatactcta tactaagagc
     8101 ataattaaat aagacataaa aacattgatt ttaaagcttt cttggcggtc tgcatttaca
     8161 tatcaatagt gttttttgtt gtctttttag ttgttaaatc tgtttacaag atatagtata
     8221 gctcattact cagaattagt caaattattt ctgcgtattt ttctaaactt tttttgtaaa
     8281 catttagaaa ggttgtttta tcgatgaata cctatgaaat aataaaagac ttggcgaaga
     8341 ggaaaaagat atctatacgg cagttagaaa ttaattttgg atattctaac ggttatctag
     8401 caaagtggaa aaataatact cctaattcag atgaattaag tcggattgct gactatttcg
     8461 atgtctctgt agactacctt ctaggtagag aagaaagaga aacccctaaa catgtggatt
     8521 tatcagaaga cgatactgtt ttttcttttg atggaaaaga aatatctaag gagacaatgc
     8581 gtaaagcgat tgcaattgct aaagctttag aggaaaatga atagttggag tgatggtttg
     8641 tatgtatttg aaattgaaag aaatgttgag cgaatacaat ttgaaagtag tttatatgga
     8701 gatgaaagag cctggtttct actatccaaa acccagaatc atttttttga atgaaaatct
     8761 atacggtgaa actgcagaag cctttcattt atctcatgaa cttgcacatt tcagcgcctc
     8821 tcattttgaa ttttcagttt tgtacgatac ttctacaact tttcactcaa aatttgaaac
     8881 agaagcagat aaaatagcta ttctaatttt attaaatatc tatattgaga acgaactgac
     8941 tgatgaatct cagtttaatc tcgaaaaatt tatggagtat tattctattc agaataagct
     9001 cagatacact tgttatgctg tctgccaatg ctattttaag aaaaaatatt cttacgcaag
     9061 gcaatatgta tgaatacatc aagaattatt aaaaaaaccg atttcagcaa aacattaaaa
     9121 aagcccgtgc tgcaacacgg gctctttcct catatatgag cttctacaaa aaaaatcata
     9181 tcacagaaat gaggaagaaa aaatggaaaa agagatttta ataaaatctc caggaaaaac
     9241 cttgattaaa gtaactgatg attctatttc aattattaga aaaggattta taaatttagt
     9301 taatcaaggt attaaaggag aaaaaactat cccctttaaa aatatatcag ccgtacaatt
     9361 aaaaaaacca ggtatgagta atggttatat acaatttacc ttgctaggcg gaaacgaaag
     9421 tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc gttatgttta caaaaaaata
     9481 ttggaatgaa atggaaaatt taaaaaaata tatagagaag caacaatcta tattagataa
     9541 taattccaaa gaaacacaaa tttcaagtgc agatgaaatt aaaaagtat
//
LOCUS       DAZUKA010000108         9544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000108 DAZUKA010000000
VERSION     DAZUKA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.111"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..1097
                     /locus_tag="ACG8PN_002140"
     CDS             99..1097
                     /locus_tag="ACG8PN_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4862275.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1209..1718
                     /locus_tag="ACG8PN_002141"
     CDS             1209..1718
                     /locus_tag="ACG8PN_002141"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4862276.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1748..2407)
                     /locus_tag="ACG8PN_002142"
     CDS             complement(1748..2407)
                     /locus_tag="ACG8PN_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4862277.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2409..3173)
                     /locus_tag="ACG8PN_002143"
     CDS             complement(2409..3173)
                     /locus_tag="ACG8PN_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4862278.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3181..3858)
                     /locus_tag="ACG8PN_002144"
     CDS             complement(3181..3858)
                     /locus_tag="ACG8PN_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4862279.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3895..5280)
                     /locus_tag="ACG8PN_002145"
     CDS             complement(3895..5280)
                     /locus_tag="ACG8PN_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4862280.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5490..5574)
                     /gene="ffs"
                     /locus_tag="ACG8PN_002146"
     ncRNA           complement(5490..5574)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8PN_002146"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5597..6112)
                     /gene="tadA"
                     /locus_tag="ACG8PN_002147"
     CDS             complement(5597..6112)
                     /gene="tadA"
                     /locus_tag="ACG8PN_002147"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4862281.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6218..7075
                     /locus_tag="ACG8PN_002148"
     CDS             6218..7075
                     /locus_tag="ACG8PN_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862282.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7156..7491)
                     /locus_tag="ACG8PN_002149"
     CDS             complement(7156..7491)
                     /locus_tag="ACG8PN_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862283.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7558..8928)
                     /locus_tag="ACG8PN_002150"
     CDS             complement(7558..8928)
                     /locus_tag="ACG8PN_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4862284.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9006..9542)
                     /locus_tag="ACG8PN_002151"
     CDS             complement(9006..9542)
                     /locus_tag="ACG8PN_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4862285.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2793 a         1971 c         1613 g         3167 t
ORIGIN      
        1 atacattcta taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca
       61 attaaattct gatatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa
      121 tcatcggtgc aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta
      181 aaacaaaaat cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg
      241 aaaaatatat ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc
      301 aattagaaaa acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct
      361 cgcttactcg tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca
      421 aagcagtcat tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata
      481 atgctgaatc atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg
      541 ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt
      601 tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg
      661 aacatagtgt atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg
      721 atggattatc aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg
      781 acgagacgat tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt
      841 tagaacatct ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag
      901 atatgtctac ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa
      961 aagtgaaact gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac
     1021 actatattaa tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa
     1081 atcttcgtcc tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt
     1141 ttcttgcatt tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga
     1201 gcaataatat ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg
     1261 aacgtggcgt aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata
     1321 tttctgattt gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg
     1381 aagtacataa cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat
     1441 tgatgagtcc gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga
     1501 tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata
     1561 tcgacaaggt gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc
     1621 ataccttctt agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac
     1681 acaaagaacc agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa
     1741 cgtctattta gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa
     1801 gaaaaacagc aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa
     1861 attcttctgg aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt
     1921 tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa
     1981 aaccgaaaac aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc
     2041 gatcattctt ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca
     2101 cagcataatc cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag
     2161 aaactgggaa atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta
     2221 ggtgatcaaa atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca
     2281 aatgtccgat atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac
     2341 tgatcagtgt cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat
     2401 ttttcatatt agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc
     2461 aacccaggta catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta
     2521 ttgcgtatcc cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct
     2581 ttttgcagcc ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg
     2641 tagatcggcg gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga
     2701 agaaggccgc gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct
     2761 ttttgaatag caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct
     2821 accactacat agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca
     2881 atcaatcctg cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa
     2941 tcgattgttg ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca
     3001 tctgctagac gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag
     3061 aaaggtaatt ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt
     3121 gtcccacgat aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca
     3181 ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg
     3241 ttcttgtttt tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac
     3301 cggttttttg cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg
     3361 gttttttttc ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat
     3421 gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat
     3481 tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg
     3541 ttcataaccc caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc
     3601 tccaagcttt tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc
     3661 acctatcaaa aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc
     3721 tttaggctcc tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc
     3781 ctgttcagct gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc
     3841 attttgtttt gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt
     3901 gattgggtag tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa
     3961 aaattcattt tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc
     4021 tacggtagta aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg
     4081 atggttaata gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg
     4141 aaatcccatt cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa
     4201 aactaagcgg atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg
     4261 caacgcagtg tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag
     4321 accactgttt aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac
     4381 ttggataaac gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac
     4441 ttctttttct tttaataatt catgcccacg ttctagacaa ctatttattt cttgttcatc
     4501 ttctgtaaaa gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt
     4561 tttctcttca tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc
     4621 tgctatttgg acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc
     4681 taaaataaca tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag
     4741 atgactcatc aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa
     4801 aatatcataa ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc
     4861 agtaaatgca attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt
     4921 cgtccatgcc ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa
     4981 ctcagcatga tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt
     5041 tcctatcgtc gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt
     5101 cgttgcttct gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt
     5161 tgtcgttttt tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct
     5221 tggccaattt tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat
     5281 gtaccctctc gcctctcttt atctttatca taccataggc agctttttct tccattgaaa
     5341 caagctctat ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat
     5401 aaatcagtct ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa
     5461 aacagacccg aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta
     5521 gtgctgcttc cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg
     5581 caaagaatag tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc
     5641 gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc
     5701 caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac
     5761 cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa
     5821 tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca
     5881 agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag
     5941 tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata
     6001 caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg
     6061 caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt
     6121 ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt
     6181 tttttctatg gtaaaataaa tacaagaggt gaacaagatg aaaatggata aattacttgc
     6241 tctttaccca caagcacaga aaaaaaactc tccagcatca gaagcggata tcctttcttt
     6301 tgcaattgat gatgctttta tctggatcaa acaagattca ttatctcaac aagaaaccag
     6361 ccttttgaaa gcgttattcc ctgtaatcaa cgatcaaaaa aaacacccat ggtatcacta
     6421 tctttttaaa gatgcttctt gtgttcaaga aaaaagcttt cgtttgatcc agcttcacgt
     6481 cgaatcaaaa ggagaattct taaaaaaaga atggcaagat actattttgg aattatttcg
     6541 ctattctgct gatttctttt tttatacaga cacagatgcg ctcttagtcg aacaaataaa
     6601 ccgtgattat cttgatgcta ctgaaatcaa cggtattttt ctatctttag atgctgattt
     6661 tgatacgaca acgtctgtat tcgttggttc tttccattca cctggcaccg acctcgtccc
     6721 tttgtttgct gaagaaagaa aaattttttt aacagagcaa aacaatcttt atactcacag
     6781 ccgtactttt tctatgcaag atgtggcttt acattactat acaaaagata ctgtaaaaga
     6841 tagtaagttg atcaaaagtt acatagaact gatcaaacaa aatgaaatgg aagtgatcat
     6901 tcaagaatta tggaatgaat taggcaatgt cagctctgct gcaaaaacac tgtttcttca
     6961 tcgtaatact ctaaaatatc gaatcgaaaa atttcaagaa cagtctggtt tcaatctaaa
     7021 aaaagccaac gatttactgt tttgctatct tttattgctt catgaacata attaaagata
     7081 gattaaaggg gttgtgacaa taattttgtc acaacccctt atttcgaatg aatagtgttc
     7141 aaaagctagt ttctcttaca tcattttttc aacaaaatgc attagccggt cttttacatc
     7201 ttcggttgaa tctttgattg aatcaccata aacagagatt ttcttttttc ctgatttgat
     7261 ttttgctaac attcccatca aagaatccaa gtcactatct gaaagaccat ctacgatttc
     7321 tagtaatttt tcatttccat caaatttgtc atagacgaat ttttttactt tcgtccgagt
     7381 agtcatatga tggatctttt ccattacttt tcccgataca acgactgctg tcgctacact
     7441 ggcagcggct gctacgctta atccgattgt tacttttgtt gatgttttca taagtatcag
     7501 ctccttttta tctatctcca tcatagcata gacaaaaaaa taaacgaact aatccactca
     7561 gtgaaatctc aatcctttag ggaaaaagtt ctttactgtt ccttttactt gtttcccaaa
     7621 tccgacaggt atcttatcta atactagcaa aacccatcct tgattcccat cacgttgaaa
     7681 tgtttctcct gctgtgtaag attgccattc cttttgagtg atcggcaagc aaggtatatt
     7741 ctctatcttt tttgtagcaa gcgctaatgc atagctaggt tcaaatcgat tctttttgaa
     7801 atctcctaaa tgcaaacctg tacgtacgac ttttaaacca tcgagagaag gggctaactc
     7861 aggaacttcc caaagatggt cgttgaacac taaaagtctt ccagtagctt cataatgaaa
     7921 atcatttgaa aactccgtcc agagtttttc ctgctctttt gtcatttgca ctttactttt
     7981 ttttcgtgtc ttcttttctt tatgcatctg attttgtcca tgaaaagtca gctttgcaac
     8041 aaaatgtcct tcaccctgat ctttatgagg ccatatacgt attgtttttt ccaaaccagc
     8101 tacgcttccc cattcagaac gtcctgaact aacggattga gtcaaaggaa tctcctcaat
     8161 cgttactgga taattttcta caagccagct aatgatctct tcattttctt ctggtgcaaa
     8221 cgtacaagtc gaataaatca gttgtccttt attttttagc atttttattg ctgaagacaa
     8281 aatctcttgc tgacgttttt gacagtatag cggagattct tctgtccact cttttattgc
     8341 atttggatct tttcggaaca ttccttctcc tgaacaagga gcatcaacca cgatccgatc
     8401 aaaaaagccc gaaaaatgag ggacaagttc tgcgggtgca tgatttgtca ctattgcgtt
     8461 cgaaactccc catcgttcta tattttctga taaaatcttc gcacgtttcg gaaagatctc
     8521 attcgtcact aataaccctt tccccttcat ttgagcagct aattgggttg atttaccacc
     8581 gggagccgca cacaaatcca aaactttttc accgggtttt gctgcagcag ctgttccaac
     8641 gatcattgca cttggttctt ggctatattc atacccagct tgatggagga aagactttcc
     8701 attgacagtg ccaagaaatc cctcattgct gtagggagcg ggttgtaatt cttcgctatg
     8761 gtaagtttgg accatgtcca atcctgccgg ttttagtgga ttccaccgaa atcctttttt
     8821 cacgcttcct tgttcaagcg cagaaaaaaa atcggatgct tcttcgccta aaagaagacg
     8881 atattttttg ataaattgct gtggtaacgt tgtcgcttcc tccttcattc caatgtttcg
     8941 ttagacattt tacaatcttt tttacgaaaa ataaaggtgg ctgatctact ttttcatgtt
     9001 gctgcctaag gtgactgttc ctttaagata ttttccaagt attcatccgt cacctcgttt
     9061 ttatcccaaa cagcgttcat catttcaaga aaatcttgag aagaatcatc tgagcgcaaa
     9121 cttcccaatg cattaagcca taccaattca agcgactttt cttctttgat cttcgtgtga
     9181 cctttgacat atgagaaaaa tggccgctta aatagcgaat ccatgtctaa atcagatttt
     9241 gcaatgattt cacgaatact ttcatggata gaaaaaaaat ctttcaataa gtagacaatg
     9301 cttaaaacat catcggaact gcattcagct aagtagtttt caaaataaac agtgatttct
     9361 tctaatatct tctcttttaa cccgctgatt gatttgaaat gtgtataaat cgggtatgta
     9421 gaacaaccgc agaactctgc taactctctt gcagtaattg acttcattcc atattcttcc
     9481 acgtatacca tacttttgaa cagcaaatat tctttactat aatttgttac tctccccatc
     9541 attc
//
LOCUS       DAZUKA010000109         9395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000109 DAZUKA010000000
VERSION     DAZUKA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.112"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..794)
                     /locus_tag="ACG8PN_002152"
     CDS             complement(126..794)
                     /locus_tag="ACG8PN_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4862286.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            928..1215
                     /locus_tag="ACG8PN_002153"
     CDS             928..1215
                     /locus_tag="ACG8PN_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862287.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1631..3169
                     /locus_tag="ACG8PN_002154"
     CDS             1631..3169
                     /locus_tag="ACG8PN_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4862288.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3166..4131
                     /locus_tag="ACG8PN_002155"
     CDS             3166..4131
                     /locus_tag="ACG8PN_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862289.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4118..5440
                     /locus_tag="ACG8PN_002156"
     CDS             4118..5440
                     /locus_tag="ACG8PN_002156"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4862290.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5503..5607
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5681..6424
                     /locus_tag="ACG8PN_002157"
     CDS             5681..6424
                     /locus_tag="ACG8PN_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862291.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6439..7431
                     /locus_tag="ACG8PN_002158"
     CDS             6439..7431
                     /locus_tag="ACG8PN_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4862292.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7443..8180
                     /locus_tag="ACG8PN_002159"
     CDS             7443..8180
                     /locus_tag="ACG8PN_002159"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862293.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8170..8892
                     /locus_tag="ACG8PN_002160"
     CDS             8170..8892
                     /locus_tag="ACG8PN_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4862294.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8976..>9395)
                     /locus_tag="ACG8PN_002161"
     CDS             complement(8976..>9395)
                     /locus_tag="ACG8PN_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4862295.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3080 a         1612 c         1976 g         2727 t
ORIGIN      
        1 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
       61 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
      121 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
      181 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
      241 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc
      301 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
      361 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
      421 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
      481 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
      541 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
      601 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
      661 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
      721 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
      781 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
      841 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
      901 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
      961 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     1021 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     1081 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     1141 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     1201 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     1261 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     1321 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     1381 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     1441 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     1501 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     1561 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     1621 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     1681 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     1741 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     1801 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     1861 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     1921 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     1981 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     2041 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     2101 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     2161 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     2221 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     2281 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     2341 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     2401 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     2461 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     2521 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     2581 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     2641 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     2701 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     2761 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     2821 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     2881 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     2941 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     3001 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     3061 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     3121 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     3181 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     3241 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     3301 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     3361 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     3421 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     3481 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     3541 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     3601 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     3661 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     3721 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     3781 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     3841 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     3901 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     3961 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     4021 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     4081 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     4141 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     4201 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     4261 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     4321 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     4381 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     4441 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     4501 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     4561 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     4621 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     4681 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     4741 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     4801 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     4861 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     4921 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     4981 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     5041 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     5101 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     5161 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     5221 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     5281 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
     5341 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
     5401 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
     5461 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
     5521 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
     5581 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
     5641 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
     5701 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
     5761 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
     5821 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
     5881 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
     5941 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
     6001 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
     6061 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
     6121 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
     6181 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
     6241 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
     6301 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
     6361 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
     6421 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
     6481 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
     6541 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
     6601 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
     6661 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
     6721 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
     6781 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
     6841 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
     6901 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
     6961 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
     7021 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
     7081 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
     7141 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
     7201 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
     7261 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
     7321 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
     7381 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
     7441 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
     7501 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
     7561 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
     7621 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
     7681 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
     7741 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
     7801 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
     7861 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
     7921 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
     7981 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
     8041 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
     8101 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
     8161 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
     8221 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
     8281 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
     8341 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
     8401 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
     8461 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
     8521 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
     8581 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
     8641 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
     8701 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
     8761 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
     8821 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
     8881 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
     8941 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
     9001 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
     9061 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
     9121 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
     9181 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
     9241 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
     9301 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
     9361 ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAZUKA010000110         9367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000110 DAZUKA010000000
VERSION     DAZUKA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.113"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..663
                     /locus_tag="ACG8PN_002162"
     CDS             16..663
                     /locus_tag="ACG8PN_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4862296.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            747..1691
                     /locus_tag="ACG8PN_002163"
     CDS             747..1691
                     /locus_tag="ACG8PN_002163"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4862297.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1826..3277
                     /locus_tag="ACG8PN_002164"
     CDS             1826..3277
                     /locus_tag="ACG8PN_002164"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4862298.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3298..4134
                     /locus_tag="ACG8PN_002165"
     CDS             3298..4134
                     /locus_tag="ACG8PN_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4862299.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4134..4793
                     /locus_tag="ACG8PN_002166"
     CDS             4134..4793
                     /locus_tag="ACG8PN_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4862300.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4919..5608
                     /locus_tag="ACG8PN_002167"
     CDS             4919..5608
                     /locus_tag="ACG8PN_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862301.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5614..6570
                     /locus_tag="ACG8PN_002168"
     CDS             5614..6570
                     /locus_tag="ACG8PN_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4862302.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6614..7126
                     /locus_tag="ACG8PN_002169"
     CDS             6614..7126
                     /locus_tag="ACG8PN_002169"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862303.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7382..7930)
                     /locus_tag="ACG8PN_002170"
     CDS             complement(7382..7930)
                     /locus_tag="ACG8PN_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4862304.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8019..9200)
                     /locus_tag="ACG8PN_002171"
     CDS             complement(8019..9200)
                     /locus_tag="ACG8PN_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4862305.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3111 a         1712 c         1886 g         2658 t
ORIGIN      
        1 aaggggatcg aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt
       61 ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc
      121 gaacagcgct cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc
      181 cagacaaaaa gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt
      241 gcgatcgaat tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat
      301 agtgattggg gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa
      361 gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga
      421 gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg
      481 attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg
      541 gatttggata aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa
      601 agtacatcag tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat
      661 taagaatttc ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa
      721 ataacttgtt tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct
      781 ttgccaatcc tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg
      841 aactggctca ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa
      901 aaggaaatcc cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac
      961 ttccaaactt aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg
     1021 aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg
     1081 aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt
     1141 gtcccaatgt gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac
     1201 tgaaagaagt attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg
     1261 attttgctca ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca
     1321 atgctatcaa cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca
     1381 tcaaaccaaa agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg
     1441 cgaatgttcg cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg
     1501 ggattcggac aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga
     1561 ttggtaccga gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa
     1621 cgcagattat gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca
     1681 ccatttcata gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca
     1741 ctcgtctgtt cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa
     1801 taacagaggc aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa
     1861 agaaaaagaa tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca
     1921 aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct
     1981 attgattccc cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct
     2041 cggtcaagaa aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg
     2101 agcttctcat acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg
     2161 acaaaccaat gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc
     2221 agcaagaaac ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc
     2281 tgcagtttca tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag
     2341 aatacccgct tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt
     2401 tggtccatat gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc
     2461 tatgagagac acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata
     2521 tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt
     2581 ttgtgttgat tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa
     2641 agctgtagat ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga
     2701 tggtcgtaaa ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat
     2761 ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc
     2821 ttggactttg ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca
     2881 tgtatgggat gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct
     2941 ttcaccaacg actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat
     3001 aagagcacag atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc
     3061 ttccatgttt gaggagagct tgaagatcac accttataca cagttggcta atctgactgg
     3121 acaaccagca atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca
     3181 atttatggct gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc
     3241 aaatcatcag ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg
     3301 aagaaaaaaa gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt
     3361 acagctgccg ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta
     3421 gtagtagcga cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta
     3481 gttggttatg atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc
     3541 tttaaagaat acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt
     3601 gctgcgaacg attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact
     3661 ttcaaatatt catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca
     3721 cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta
     3781 gcggaaaaac acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta
     3841 agcgatgtag caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg
     3901 gccgtatctg ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa
     3961 aacgaaagtg gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc
     4021 gtattaaaag agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa
     4081 actgatgttt cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact
     4141 ttcctctaat tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg
     4201 tatacacatt agggattggc ttcgtgtcgt ttcttttagg aaatctggta gggttgcttt
     4261 tgacagtttt aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat
     4321 cgttctttag aggcattccc gcactagtcc tgctgtttct gttatatttt gggttacctt
     4381 atcaattaag tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta
     4441 ttggtgaaat ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt
     4501 tagcttctgg tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc
     4561 gaatctcagt accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag
     4621 cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat
     4681 tcgattattt ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga
     4741 tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca tcagtcaatc tgaaaaatag
     4801 aaatggtgct agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag
     4861 tattttaaaa ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat
     4921 gaagaccccg ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg
     4981 cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag
     5041 ctgcttgaca aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa
     5101 agcagatttt cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt
     5161 accaagtaaa ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt
     5221 gtatcgtccg tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa
     5281 aaaagcagca aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc
     5341 ttctttattc agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc
     5401 aacagatcaa ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa
     5461 cgaacgatat tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca
     5521 agaagaagtt atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg
     5581 tcggttgcct acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa
     5641 caatttgacc tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc
     5701 caaccagtga tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt
     5761 caaaaaaagc accatcgaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta
     5821 ttcgatcagc atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag
     5881 atcttaacgc cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc
     5941 gatggacagc actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg
     6001 aaatttttga taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg
     6061 atccgtgact cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac
     6121 ttttttagtg atgactatct ttttttccac gcagacggat atattggatt ttctgatttc
     6181 acgatcgaag gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat
     6241 ctaacgtata ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac
     6301 gactctgcta aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg
     6361 atcatgcgtc accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt
     6421 taccagcaac aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat
     6481 ttggaactta ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa
     6541 gttcaggaac tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa
     6601 aagaggaaac tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga
     6661 aatcattaat gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg
     6721 gcagaaacca acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt
     6781 aatagtggaa gatcaaattg ctggaacagc tgcattagtc tcaagcgtgg atcccgttta
     6841 cacaaaaata gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt
     6901 cgctttagca gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt
     6961 gacgattgca gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga
     7021 accgatggaa aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc
     7081 ttttgaacac ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct
     7141 gagcaaagat aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt
     7201 gtaacagaag tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt
     7261 tttgtggaat tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga
     7321 ggtcgtgaca tagcttttgt cacgacctct cggcttatcc tacgagtatt attcgataca
     7381 tttatatcaa taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg
     7441 acacgactaa aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat
     7501 aaaccgccag tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt
     7561 gcaatgtttc tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat
     7621 ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct
     7681 ttttaatttc tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa
     7741 actcatttcc cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt
     7801 gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct
     7861 gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc
     7921 aataattcat tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat
     7981 ttccaaagct tttaaaagta actttgcttt tttgtatatt ataccagcaa gccagtcaca
     8041 atagcactta aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg
     8101 acaagatttc ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa
     8161 gagaagttgg caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa
     8221 tggaaattac catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc
     8281 gccatgatac taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga
     8341 ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata
     8401 gcattgatca tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc
     8461 acatggaaac catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt
     8521 tcttcaatga tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc
     8581 agaatataac cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc
     8641 agtgccatat aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg
     8701 gtgaacaaac gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc
     8761 gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg
     8821 attttactta atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt
     8881 agaataccga taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt
     8941 gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca
     9001 agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt
     9061 aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg
     9121 attgcttttt tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata
     9181 atacctaata aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt
     9241 tttgcaaata aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca
     9301 ttcattttcc attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt
     9361 agtaaag
//
LOCUS       DAZUKA010000111         9312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000111 DAZUKA010000000
VERSION     DAZUKA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.114"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..491
                     /locus_tag="ACG8PN_002172"
     CDS             75..491
                     /locus_tag="ACG8PN_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862306.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(508..1245)
                     /locus_tag="ACG8PN_002173"
     CDS             complement(508..1245)
                     /locus_tag="ACG8PN_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862307.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(1667..2230)
                     /locus_tag="ACG8PN_002174"
     CDS             complement(1667..2230)
                     /locus_tag="ACG8PN_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862308.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(2217..2744)
                     /locus_tag="ACG8PN_002175"
     CDS             complement(2217..2744)
                     /locus_tag="ACG8PN_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862309.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(2767..4140)
                     /locus_tag="ACG8PN_002176"
     CDS             complement(2767..4140)
                     /locus_tag="ACG8PN_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4862310.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            4224..4703
                     /locus_tag="ACG8PN_002177"
     CDS             4224..4703
                     /locus_tag="ACG8PN_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862311.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(4907..5050)
                     /locus_tag="ACG8PN_002178"
     CDS             complement(4907..5050)
                     /locus_tag="ACG8PN_002178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862312.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(5053..5370)
                     /locus_tag="ACG8PN_002179"
     CDS             complement(5053..5370)
                     /locus_tag="ACG8PN_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862313.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(5481..5663)
                     /locus_tag="ACG8PN_002180"
     CDS             complement(5481..5663)
                     /locus_tag="ACG8PN_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862314.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(5700..6500)
                     /locus_tag="ACG8PN_002181"
     CDS             complement(5700..6500)
                     /locus_tag="ACG8PN_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862315.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(6637..6921)
                     /locus_tag="ACG8PN_002182"
     CDS             complement(6637..6921)
                     /locus_tag="ACG8PN_002182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862316.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            complement(7052..7360)
                     /locus_tag="ACG8PN_002183"
     CDS             complement(7052..7360)
                     /locus_tag="ACG8PN_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862317.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            complement(7522..8052)
                     /locus_tag="ACG8PN_002184"
     CDS             complement(7522..8052)
                     /locus_tag="ACG8PN_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862318.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(8136..8432)
                     /locus_tag="ACG8PN_002185"
     CDS             complement(8136..8432)
                     /locus_tag="ACG8PN_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862319.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRDIMVQS"
     gene            complement(8438..9181)
                     /locus_tag="ACG8PN_002186"
     CDS             complement(8438..9181)
                     /locus_tag="ACG8PN_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862320.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
BASE COUNT         2793 a         1871 c         1181 g         3467 t
ORIGIN      
        1 atttaatagc ctaaaaaggc ttgttagata aggaatatct aactaatcaa aacaaaaaag
       61 gatgttaaga aaaaatgaac aaaaaaagga aattatcagt attaattaca cttgtttcta
      121 tattgactat tcttagcgtt atcttgtcgc tgaaaatatt caatcaaata aacacaagct
      181 caaatgccaa agatggaata gtcgtagaaa atacctctaa ttctaaagaa actggtacta
      241 ctacaaaaaa agacaaatct gctgagatca ctacgaaaaa ttatttaata aagactgttt
      301 attttgatga acgaaaaaca gcaattctca aagatgagga aggatcagaa ttaaagctac
      361 ctataacggt atccgataag ttcaatggaa cgttctcctt acaaaccgct gaaggatcac
      421 ctgacaatct caaagttggg caaacaatca ttcttggtaa aaacgacgaa ctgtattcat
      481 ttgtaaaata aaaaaatgga gcaaaggcta gtcaagccct tgctccattt tccattcttc
      541 aagaggtgta gctatttcaa caccaaagtt taaatactgt ggatgctgta gatacatgat
      601 atccattcta ttttcgataa actcttttaa cgttgagtag atgtattttt ctaatgctat
      661 atctgcccaa ctaccataat caagtttatt cgtgatcttg acgtttattt caatttgaga
      721 aacatcaata ttttgaacat caggaaactt attcagtctt agctcttctc taaccgcttc
      781 tactagcggt tcatattgtc cgcctagttc gctaattgtt gaaactattt catctgaatt
      841 ccaacgatct tccaatacct ttgctacatt ttctttaggc ggttgtcttt ctataaattc
      901 ttcaggtgtt tcatcaaact ctactctaaa gttcaattta ttttgattgt tagcatacat
      961 ataatcatct ctgtttgatt caaaaaagtt acgaattcct gtgtctgatt tatcacttaa
     1021 ctccttattt gctaaagtag cccaaaaatc ccaagttcct ttattttcag tatacaaagt
     1081 aaaattaatg tttatatagt ttgcagtttc aggataccct tgctctctaa gagcatcttt
     1141 aacaatcact gctaagtcag tttcaaaatt ctccataaaa aattcactaa attcttttgg
     1201 attccacgtt tcttctaata acttttctaa tttatgatct gccattatta ttctccccca
     1261 ctttactttc aatcttatat ctaaaactgc ttaaaattaa gagctatacc ttatcataaa
     1321 aggtatttta tgctttattt aagtctacaa ttctatatta caattatcat ctagaccatt
     1381 ttcttatgtc aaataaacca tatatattgt ctttaatagt tttatttgac tttttaagaa
     1441 agtatttact tttgactttt tttacgtttt acaacatata cgtattgtaa tgttcagctc
     1501 aatttattat aatctaaatg aattgattaa taaaaattct gaggttcggg ttttttatca
     1561 attcttttct gtttgtgttt gtgatgaaac aatccttttt cggtaggttt tgttatcatc
     1621 gtcagcacaa agagctaacg ttggtaccgt tagctctttt tttattttac tctgctctta
     1681 catagctatc taaatcctcc aaataaaaca attgattttc tcctatgctt gttactccac
     1741 gccaaattgt tgttgttgct gttgttggag tatttcttgt ctgtgtaatc aattcaacca
     1801 ttttaccttt ttgatttgtt gtatagtaat cagcagtata gctagatatt ggactattac
     1861 cagtgcctac catcgtgcca gaaatatcaa caggactaga ctttactatc tgaggaaatt
     1921 ctttttgtat ttccttcata ttcttcgtat atttttccca tgaattccag ttgtaaaata
     1981 aattaaagaa tttcctactt tgttccttaa tttcagcatt tcccaattct ttgtattgag
     2041 cttcctgcat agctttcact ttctggttat tcttgactac ttcgatttgt ttacttttaa
     2101 ggtcttccaa ttctttgaca ttattatcat aagcattttt cttttgaaat agtaccacac
     2161 aacaaaggat cacaattatg gaaagtattc cgatcgcctt tttagcagta gtgatttcat
     2221 gattttgcat aaaaagcacc cccagtcact ttcttatcat tatttagtgt gatggttaat
     2281 gtggtatcat acactttttt tccattgtat tgaacttcaa aatcggtcaa tgcttcatac
     2341 aacccactac tagatgtctt aattccaaag tttgttagct taaaagtaaa aggcttatag
     2401 tactcgattt tttctttctc actactctta tcagtaattg gtggtttacc tgttacaata
     2461 aatagttttc tcaaaaattc ttcacctgat tgatctgcaa acttattttg aactgatcca
     2521 ttcaacattt cttgaatatc attttctaaa tctttccgta atccatccac ctcatttggt
     2581 ttaacctctt taaagaatgt cagcttatct tcaatatgca cactgtgttt ttcagcttct
     2641 atgtcctgat tctgcttatt gaagaatagg attccaacaa gcagtgctaa taacatacca
     2701 gaaagcaaag aaatgataat tgtatctttc actttactat tcatctatat cgtccttcat
     2761 tccaatttat ttttcgattt caaatttagc ttttcctaca ccgctaaaga aatgcacatt
     2821 atctgatccg tccactccat caatcaacgt taccgaaact ccccttgttg gagcttgggt
     2881 tggtggaaca ttaaagttag cacatagaaa tgatccatct tcaaacaccg ctataactac
     2941 accagtatgc cctgcatctc caacagccca taaccatcca tccgaagcac taaacccata
     3001 acctacgatt ggtttgttcg ttatttttgc tcctgccgat ttgtagcttt cccatacata
     3061 accaccattt ccagtagtcg gttgattccc atcgtataat tggctcatat aagcccacgt
     3121 caactctgta cactgacctt gatatattcc gttaaaaggt gaagttgacc aatctggttc
     3181 tttgaatttt ggtagcgtga tcgctttccc atattttttc aaagcttctt cataagtcat
     3241 attcgctatt acttgacctt ttttaccgtt aatttttcct tctttcccaa ttgatccaga
     3301 accagaatta gcgtcatccg tagttgcaca ggcatcttct actgttcctg atgctgaact
     3361 tgatgaatta ttcgatccat ctccggtact tgaacctccg gatattttat tgatcttact
     3421 ttcatctttt gcaccgtcca tacccttgct tttcatgtac ttccagattt cttttgcatt
     3481 ttcaccacgt acagctatta cagctgattc ggaatcagct gaaatttccc agtttttatg
     3541 aaaagttact gcattttcta cgacatcatc agaagcattt aaagcataat cttttaatat
     3601 tcctacgaat cccccatcac ctttgaacat aaagtccaat tgggcttctg tagtccacca
     3661 agaaccgcca ttatttttct tagaccaatc aactaatgct gtatgtcgcc cagctgtcca
     3721 ttgaccatag ccaataccca tcagaccatc agtaaattgt ttgatgaagt caacttctct
     3781 ctgtccatct ccgtaatacc cttctactgc tcgaggatca atacctgatt cttgtttcca
     3841 gttacctaaa atacctgcaa tactagcacc tgataagcca tattctccat tcatgtattt
     3901 gtaaatatct tcaatatttt tggctacatc acctgttaca gcgccacttc cagtatcgtt
     3961 tccatcgcta tctgttcctg acccactacc ttcagtagta cagttaaatt gtgcatcata
     4021 agtagctatc ccatcagcat gtatcacttg cggatttatt gtgattacac tcattgcaat
     4081 ccctgtaaat tgcaccatta acataagcaa tgtggcaatc tttctcttag attttttcaa
     4141 taactacgcc cccaaaaagt gtactttttt atgttattat gataatataa taacataaat
     4201 taatagaaag gaaaagctaa taaatgactg ataaaacaag tgatttacct aaactagctc
     4261 caaaaacatc tcgagtaaag agcaaaaaag atggaaaaac aaaaaatgct gtaaagaaaa
     4321 agtcagtaaa agaaacatcc tcgttactac ttgatattcc aatgagttct tttgatgaca
     4381 atacggacga aacttctgtt aaaattgcaa ctcacgttgg aatgaacaat caaaatgatt
     4441 ctttttacaa cttgttaaac agcctaagag agttggaaga taaaaaaaat caggtaatcg
     4501 catcaaaagc tcctgatctc acaaccgatt tggatgctga aattatccaa atcaaacaag
     4561 atgagcgatc agttaaatcg ctactgaaaa cctactttga tggttttgat aaagaactag
     4621 ctccttatag ccaagcaatc ctaactaaac caatacaagc tccacatatt tcaatttccc
     4681 tacaaaaaaa gagtgatgat taaacaatca ttactctttt ttagatttct ggtaagcgct
     4741 gatcgcaaaa accatcaaca atattgattc aaaaaatatg ttcattcgtg tctctttttt
     4801 ccattataga tttcccacta ttccattaaa tcgaaagtca caccgattac tacaaagatt
     4861 aacgtgacca cattcaaaaa cattaaaaga tatgctgata atctcatcat ttttttagtt
     4921 cacttactgg taatctaaac atttgaacga ttgcactgga aataccataa ttagcaatga
     4981 caaacaaaaa atacgcaata atttcactta cacctagata gatcagtaaa ataaataagt
     5041 cacttaacat tgttagtttc ctcccagtat atgattttca tttttgggaa gtcttttata
     5101 atccaattcc tcaaaaaaag gaaccatagt gtcactaata taggttttag tttcaggtac
     5161 cgccaaaatt ccccaatcaa gaatttcttc ccatgataca tcatcaaatt ctaaaatatt
     5221 atgatcaact gacctattac caagttcata tccaccaaag atcaccatat tatcaatcat
     5281 gtatagaatt tcatcaggcg aaccatgagt taatgtttcg tcctcttcaa gcagttcgtt
     5341 taatctttgt ttgtattcct ctcttttcat acttttatcc cttctgactc atttttttga
     5401 attttaacat ttttaaccca agcatgacta tctttacatg ctattaattt ttatctccat
     5461 tttgatattt ctgttttttt ctactcacca atcaaccgta aatcctcaat attctcacct
     5521 tcaagcgaat atgaatatac ttcgccgtaa atattttcta taactacgac ttcttcttca
     5581 aagtctatgg actgcacatc aaaccattct cctccgccaa attcgtctat gattagtacc
     5641 tgcaatattt tatttactgc catttcaatt tccccttata ccattctatg tcttccaaac
     5701 tatttttctt ctttccatac caaggtttca cgtatctcaa aattttcttt aaataagtgt
     5761 tccggtactt ttataattaa ctcttttcct acttttatca gatagtcttt ttcacctaat
     5821 gagatttgac cagtcggtaa ctgtatagaa actcttttag ttttattaaa ttttttagtt
     5881 ttcaatacag tatacggtct acagaatttc tctaagcgtt caaatctttt actatcgtat
     5941 tggaaataag ctttaggaac agataaagta caaaaactat cggtaacagc acttatgtct
     6001 tttttagaaa ttatcccaac tttagaactt ctaaggaaag ttgctaattt cttatatcca
     6061 ccatgacttg tttcaatata gatgctattg tattggctgt cgtctatctc atttattgtt
     6121 tcaacctttt tcctgacagg tgtttcaacg agttctcgaa cctcttcttc actgtacgct
     6181 aatacaatta actcttcccc atttaccatc tttagaacat acgcttgctt cttttttctt
     6241 ggcttatttt gagattcaac ataaccagac atggaatctt tatacttcca agcatgtact
     6301 tttttctcta atttctgtat ctcttttttc agctgtagat attcagacat tttttcattg
     6361 taacgttcgc aatataaaca atattcatct tttccaaaat catgatcaag tccttcttca
     6421 atatgtttat tcaacaaacc ggaaaaatac tgatcatact gcttctttcg taacttcagc
     6481 tctcgtaaat cttcagacat ttcatttttc ctttccaatt attggtgcca tttcacaatt
     6541 tgggattgac taaacaccat aattaatttg taagctaaca caattacaac tgttggtaat
     6601 tgtgttagtc tccatcaact tgtctggtgg ctttttttat tgcacaaatt tcatagctct
     6661 caaaaagcgc ttggcttgaa aaggtgttat tctacgaacc ttattcgcca atttccaatc
     6721 tgataaagca atttgccatt cgtgcatttc aaaatccggc atgatttctt tgatatactg
     6781 tagctctttc ttttctcttt cttttaagaa acggctcatc gagtttaaca ttggaactaa
     6841 ggatgcgtag gtacttgaat aacttccaat gcgtttcgta gtaattacat aatcaataaa
     6901 aacactatta agaaaggtca agtttataaa ctctgattca tacactctaa aatttgcagt
     6961 taatcgcttc gccttgtcac cccaatagtc acttttgttg taggcttttt cccctcgaat
     7021 atagcactct gtttctcttt cgtaataata gtcatacttt tttcatcatc aaatctgaga
     7081 gtttttgatt atctttttca ctgtaaaata atctactcgt taaactttta ggataatctt
     7141 tgatagaata tgcatcccaa acaattcttt gaacatattc acgatctata ttttctctga
     7201 cttcctcact ccaataatgt tgaccgtaat agcatttttc tattacgttg ttagcagaaa
     7261 cttcaaaata ggattgttcc ctttgtatcc tcataccaat catcatagcg caggaaataa
     7321 ctattaataa caatcctgtt tttaatctat tattccacat gcagtttcca catccttatc
     7381 ttgataaaaa tcctcttgac cctctccaat tactgtttta aatataacca ccaaaacaag
     7441 atcatgctaa gaaatccaag agtatataag atgatacatt ctttgttaat tatccaaaaa
     7501 actctgattt ctttgagctt ttcacgcatt aggaaacacc ttgttatatc ttttttgtat
     7561 ttctttcaat tgttttttaa aactcataaa caactgttca tgatctggtc taagcgttag
     7621 aatatctaaa ttatttatct tttttaattg tgtaataaaa tctattttgg attttctttc
     7681 aatatcaaaa ccgacaataa tctttcctgt tggatcaacg catttcactt gaataacttt
     7741 gtcattttgc gatagctcta atattgatga tttttctttt gcctcttcca aaagatgttc
     7801 taacacttcg ttaggtgttc catgatagta ataaatcatg gtcttctcga aaatttttat
     7861 cggatcaaca ccatattctt ttccgattcg ataaaaaaat ttcattggaa tttcttccgg
     7921 atttctgttc ctgcttgacc agttagagat ggtttgataa tttgcaaaat ggattcgttc
     7981 cgctaatctc tgaactgtgg tctctaaatc ttcttctatt aattgtttta cagcactaat
     8041 gttttgtgac atttgctttc tatcccttca aattcgttgc attatcaatt ctaagatttc
     8101 atgttttagt gcatttctat ctacattttg cttttttaac tctgcaccat aatatctcgc
     8161 aatcccttat ccaagcatac aattccttga tctaatagat catattccgc tttttcaaac
     8221 cttcgctttg cctgctcaac aagcaaaatg atattgtgat gtatctgatt aaactgccga
     8281 tcatattcct ctaaagtttt agcatctaag ttagcaatat gatcctctgc taatttttgt
     8341 gttaaaaata attcgacttt ctcagatttc aacatctgta ttttttcata ctcttgtttt
     8401 gcttggatat agtcatcttt caagctagac atctaactca tctccttgcc tgtataaact
     8461 gatacggact gtccaaaatt gaataggttg gtgatgactt caagaagact tcaccttttt
     8521 cacatgaaaa taaaccaatt aattcttcaa tttcatttac gatcagatat ttattagtta
     8581 aaattttccc attttctttt tctagctcga caatcgcttg attccatgta ttttgcacta
     8641 aatcttgaat caatttcaat ctatccactt ttttcatctc atacagggca atttcagctt
     8701 gtttaatgcc ttttaatttg cgaaaggcat atttgtattg tgtgtaggtt aaaaagcctg
     8761 tgtagttata catgggcttt cctgatgtct tgtagtcatt taagcttgga tatatttcag
     8821 ttttccattt ttcttctgaa cgttgatcaa taattcttaa tttttcaggt aaatcaattt
     8881 catagtattc ttcaattttc agctcacgat aaagtagatg tttttccttt tctttttcaa
     8941 ttacaaaaga attatttccc ttcagtttag ctatttcttc acttgtgaga ccaagcctaa
     9001 ttttttctac ttcacttcta agttcacgaa tacttgttgc ctttaaccac ttcccatttg
     9061 caccattatt gtaaccggta agtaactgta ttttataaat tccttcacca tgaagcacct
     9121 tatatatacc aacctgattc ttttgcggaa tatgagcttc atttgcactg atttctttca
     9181 ttacgcattc tcctttataa tctagttaat tagcttgtgt gtttcgagta gttgagtaag
     9241 aacgtttgtt tcagaaatca tttatttgtt atcacttaat tttaaaaaaa tagggcttac
     9301 gccctaaaga tc
//
LOCUS       DAZUKA010000112         9311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000112 DAZUKA010000000
VERSION     DAZUKA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.115"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..1394
                     /locus_tag="ACG8PN_002187"
                     /pseudo
     CDS             180..1394
                     /locus_tag="ACG8PN_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1573..1908)
                     /locus_tag="ACG8PN_002188"
     CDS             complement(1573..1908)
                     /locus_tag="ACG8PN_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4862321.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1892..2155)
                     /locus_tag="ACG8PN_002189"
     CDS             complement(1892..2155)
                     /locus_tag="ACG8PN_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4862322.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2615..3418)
                     /locus_tag="ACG8PN_002190"
     CDS             complement(2615..3418)
                     /locus_tag="ACG8PN_002190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4862323.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3396..4520)
                     /locus_tag="ACG8PN_002191"
     CDS             complement(3396..4520)
                     /locus_tag="ACG8PN_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862324.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4536..5351)
                     /locus_tag="ACG8PN_002192"
     CDS             complement(4536..5351)
                     /locus_tag="ACG8PN_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862325.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5341..6213)
                     /locus_tag="ACG8PN_002193"
     CDS             complement(5341..6213)
                     /locus_tag="ACG8PN_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862326.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6298..7587)
                     /locus_tag="ACG8PN_002194"
     CDS             complement(6298..7587)
                     /locus_tag="ACG8PN_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4862327.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7731..8243)
                     /locus_tag="ACG8PN_002195"
     CDS             complement(7731..8243)
                     /locus_tag="ACG8PN_002195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862328.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8310..8453)
                     /locus_tag="ACG8PN_002196"
     CDS             complement(8310..8453)
                     /locus_tag="ACG8PN_002196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4862329.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8936..>9311)
                     /locus_tag="ACG8PN_002197"
     CDS             complement(8936..>9311)
                     /locus_tag="ACG8PN_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4862330.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2871 a         1984 c         1487 g         2969 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaagcaa
      121 aatcaattct accttagatg ttcttatatc actctatcag tcctatttga caaagagcca
      181 agaaaaagac gttggtcgtc gatgctgttc tctcgcctac tcctcgtgct tgtagaagtt
      241 gtggttctac tgtggtagat ggaaacggga aagcaattat agtgaaaaat gggaagaagg
      301 aaacgattgt ccgttttgaa caatacaatc atatgccttt ggttacgcgc ctaaaaaagc
      361 aacgctatac ctgtaaaaac tgtcgtaccc attggacggc tcaaagttat tttgtccgac
      421 tcagccattc aatcgcagat catgttcgat ataaaattgc ttctttactg actgaaaaag
      481 tatctttgtc ttttattgcg aaaagttgtc aggtatcttt gactaccgtt actcgtacat
      541 tgaaagagtt taaaagctat ttaccaaagc cttctaagaa gattctcccc agagtattga
      601 tggttgatga atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag
      661 atggtgaaac aggaaactta attgctgtct tgcctacgcg taaattacct cgattaacaa
      721 gctatttctt aggctgcacc aatccagaag aagtagaatt cttagtcaca gacatgaacg
      781 ccgcttactt ccagcttacc aaacgtgttc tgccaaatgc gaaagtagtg attgatcggt
      841 ttcatattgt caaacacatg aatcaggcac tcaatgagtt gcgtatccgt gaaatgaatg
      901 agcttcgtaa agcaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttc
      961 tgctaaaaaa tcgtgcaagc atcaaccatt atgagtacaa aacatggaaa agtttccgag
     1021 caccaaaata cccatttctc actgaagcaa tgatgattga tcgattgctt gagttttctg
     1081 cgcctctaaa ggaggcgtat cccttttttc atgaattagt cgaagccttt cgagacaaag
     1141 accctgactt atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc
     1201 gggagaagct tcaaaacctt ctgacctatg aagaaggtat caccagcgca atgatctatc
     1261 cttattccaa tggaaaaatt gaagcgaaga atacccacat aaagacaatg aaacgagtat
     1321 cctacggatt taaatcattc gagaacatga gaattagaat ctttttgatc aatcaattaa
     1381 tcaatgtaag ataacaaaaa atctgaacca gaatgtggtt cattctgacc cggatttgat
     1441 tttgcacaac tcatcagtcc ttattgacaa agagccaaaa atctgagcca gaatgaggtt
     1501 cattctgacc cagatttgat tttgtacaac ccatcagtcc ttattgacaa agagcccttc
     1561 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
     1621 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
     1681 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
     1741 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
     1801 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
     1861 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
     1921 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
     1981 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
     2041 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
     2101 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
     2161 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
     2221 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
     2281 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
     2341 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
     2401 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
     2461 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
     2521 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     2581 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     2641 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     2701 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     2761 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     2821 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     2881 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     2941 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     3001 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     3061 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     3121 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     3181 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     3241 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     3301 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     3361 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     3421 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     3481 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     3541 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     3601 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     3661 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     3721 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     3781 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     3841 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     3901 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     3961 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     4021 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     4081 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     4141 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     4201 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     4261 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     4321 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     4381 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     4441 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     4501 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     4561 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     4621 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     4681 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     4741 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     4801 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     4861 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     4921 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     4981 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     5041 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     5101 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     5161 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     5221 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     5281 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     5341 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     5401 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     5461 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     5521 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     5581 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     5641 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     5701 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     5761 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     5821 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     5881 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     5941 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     6001 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     6061 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     6121 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     6181 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     6241 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     6301 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     6361 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     6421 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     6481 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     6541 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     6601 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     6661 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     6721 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     6781 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     6841 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     6901 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     6961 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     7021 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     7081 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     7141 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     7201 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     7261 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     7321 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     7381 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     7441 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     7501 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     7561 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     7621 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     7681 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     7741 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     7801 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     7861 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     7921 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     7981 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     8041 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     8101 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     8161 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     8221 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     8281 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     8341 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     8401 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     8461 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     8521 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     8581 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     8641 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     8701 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     8761 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     8821 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     8881 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     8941 ccacaaaaaa agctgtcctc acagacagcc agactgtaga caaaagctgt tccaaactcg
     9001 taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa caaaaaaata
     9061 aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt gcagcaaaca
     9121 ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa cgcatcccat
     9181 gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt ttgtaaattg
     9241 atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct ccccaaacat
     9301 gtctcgtgat g
//
LOCUS       DAZUKA010000113         8940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000113 DAZUKA010000000
VERSION     DAZUKA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.116"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACG8PN_002198"
     CDS             complement(13..1152)
                     /locus_tag="ACG8PN_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4862331.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACG8PN_002199"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACG8PN_002199"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4862332.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACG8PN_002200"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACG8PN_002200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4862333.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACG8PN_002201"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACG8PN_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4862334.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACG8PN_002202"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACG8PN_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4862335.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACG8PN_002203"
     CDS             complement(6166..7257)
                     /locus_tag="ACG8PN_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4862336.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACG8PN_002204"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACG8PN_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4862337.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACG8PN_002205"
     tRNA            8800..8871
                     /locus_tag="ACG8PN_002205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAZUKA010000114         8831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000114 DAZUKA010000000
VERSION     DAZUKA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.117"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(385..882)
                     /locus_tag="ACG8PN_002206"
     CDS             complement(385..882)
                     /locus_tag="ACG8PN_002206"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4862338.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(922..1731)
                     /locus_tag="ACG8PN_002207"
     CDS             complement(922..1731)
                     /locus_tag="ACG8PN_002207"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4862339.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2065..2922
                     /locus_tag="ACG8PN_002208"
     CDS             2065..2922
                     /locus_tag="ACG8PN_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4862340.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2915..3817
                     /locus_tag="ACG8PN_002209"
     CDS             2915..3817
                     /locus_tag="ACG8PN_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4862341.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3850..4200
                     /locus_tag="ACG8PN_002210"
     CDS             3850..4200
                     /locus_tag="ACG8PN_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4862342.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4249..5172
                     /locus_tag="ACG8PN_002211"
     CDS             4249..5172
                     /locus_tag="ACG8PN_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4862343.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5250..6143
                     /locus_tag="ACG8PN_002212"
     CDS             5250..6143
                     /locus_tag="ACG8PN_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4862344.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6181..6744
                     /gene="amaP"
                     /locus_tag="ACG8PN_002213"
     CDS             6181..6744
                     /gene="amaP"
                     /locus_tag="ACG8PN_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4862345.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6758..6946
                     /locus_tag="ACG8PN_002214"
     CDS             6758..6946
                     /locus_tag="ACG8PN_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4862346.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6959..7861
                     /locus_tag="ACG8PN_002215"
     CDS             6959..7861
                     /locus_tag="ACG8PN_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4862347.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7893..8135
                     /locus_tag="ACG8PN_002216"
     CDS             7893..8135
                     /locus_tag="ACG8PN_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4862348.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8391..8762)
                     /locus_tag="ACG8PN_002217"
     CDS             complement(8391..8762)
                     /locus_tag="ACG8PN_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862349.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3075 a         1530 c         1715 g         2511 t
ORIGIN      
        1 taagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc
       61 ttcttatgtt tttacttggc tgttcttatt actgcagatg caagcttttg aataccgttt
      121 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agcttaacaa acggaaaaat
      181 agttcctcat attttgccgt ttgttaagct aataccgagg gtcagctgat tgaacaccat
      241 ttaagaaaga ggtcgtgagc ctgccgtttc actctgtgtc ataaaaaagc caatctactt
      301 tgatagaaaa agcacgtagg actaaaacag ttctgcgtgc tttttttatt gtgtgttaaa
      361 tatcgtaaat gaagatagat atttttatga ttcgacgata gagacgttga aatctttcaa
      421 cagctctaaa gcggattctc cgtgttttct actggagact aacattggat caacaaaaaa
      481 gtctgcttca ggaaaagcac tctgcaggcc tatcagatta gagagtacac acaagtttgt
      541 ttcaatcccg actacttcga tctctttttg ttcttctttg ctttcaaata aagtttcctg
      601 gatttcagct aaagtttcag gtggaatcgt aaagtaatac tttttaatca ctcgttcatt
      661 ttcttgtgga gaaagaagag ggatcaattg caaatcttct cgttcttctt ctttgtcctt
      721 cagttcaact ggtatatccc gtgtgaagag aatatattca ttattttggc gtgctttagc
      781 taatctttga gcaatacgtt ctgccaattg atctgactct ggaatataaa attcgctgtt
      841 tggatcaaga atatgatttt gcatatcaat aataactaac attttctttc ctcctacatt
      901 catatcgtca ttttattctt tttaatagaa gttttttaaa atataagcaa caccagcttc
      961 ttcattcgtc agtgttactt ggtttgcaat ttcttttact tgtcgctgtg cattccccat
     1021 cgcaatactc aacccgctgt aattcaacat agcaatgtcg ttttcttgat cgccaaaagc
     1081 catgatgtca ctcgattcga ttccaaggta atccgccaga aaaacaagtg aatgagcttt
     1141 agtgatacct gtaggcgtga tttctaagaa atgcggatca gagaatactg attggatacc
     1201 atctgcttgc agatctcttt gtagcttctg tagtttttct tcactcttat ctgttaatgc
     1261 taatttaaga tagctaagag aaggatcttc caacatattt aaaatttgat caaaagaatg
     1321 gttctgtaaa ttgaaatgtt cataataagg gtcatctttg actagatttg attcatcgaa
     1381 tgtcagctga tcatcttggg tttctcggct agcaatgatg gatattttcg cttcttgagc
     1441 atagaagaag gcttttctca gtgtatcgct atctattttt gaacgtctga gaactttcgc
     1501 catttttcca tccttcattt cagcaatcag tgaaccattg gaagaaatca aatagccacc
     1561 gattcctaat tcggaaaaaa tatctttagc agaaatgctg ttccttccgg tagcgacggt
     1621 tacgattccg ccattttcat caaatttccg aagtgcttgg atattttctt ctggtatctt
     1681 tccatttcga tcaagcaagg taccgtctaa atcaatcgct gctagtttca ttttatcttc
     1741 ctactttctt tttacatatc tattttcctg ttaccttaag attaaatgag aatttaaaaa
     1801 aatgaaacta acttgctttg tcattatgaa ttttttcaaa aaacattctt aagaaaaagg
     1861 tggctatata tagtagtttt tggtcaatac ctttgaataa aaagccatga aaaacgaata
     1921 gttagcgctt accgatgcgt tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa
     1981 gcatatattt tgctttaaaa ccaaatgagg aatattctag atgtagaaca ggttagaaaa
     2041 agattagaaa aaggagtgat tttgatggag aagagatacg gcgtagttcc tgtggtaact
     2101 gcttcagacg aaaattatgc tccttacttg agcgtgatga tcgcaactgc attagaaaat
     2161 tgcaataaaa caagacgcat aaaattttat gtgattgacg atggtctatc agaatatagt
     2221 aaagaaggat tggaagaaac ggtaaataaa tattcaagta atgcaagtat ccaattcctg
     2281 acagtggaaa aagatatcta cgaagacttc ctagtcagtg accatatcac aactaccgct
     2341 tacttaagaa tttctttacc aaacttatta gctaaagagg attataagaa agtcctttac
     2401 ttagattcag atgtgttagt actagacgat atcgtaaaac tatatgatga gccgttaaat
     2461 ggaaaaacaa tcggcgctat tatcgatcca ggccaagtga aagctttgga aagattagga
     2521 atcgattccg atgacttgta cttcaattca ggagtgatgg tgattgacat tgatcaatgg
     2581 aataaaaaag aaattacaga aaaaactatc cattacctca gtgaaaatgg cgatcgaatc
     2641 atctatcacg accaagatgc tttgaatgct gtactttatg aagattggga acaacttcat
     2701 ccaaaatgga acatgcagac ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa
     2761 tatgaacgtc agtataaaga aggaaatgaa aaaccttcga tcgttcactt tacaggacat
     2821 gataaacctt ggaatacgtt gaaagatcat ccgtatacca atttatactt aaaaaaatta
     2881 gcacacagta ctctaacgaa agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt
     2941 cgcaagttgt aacacaaaat tcgtaccaca tttagctgca ttatttgtct cagtattaga
     3001 taattgcaac ccgtctaaat ttgttcggtt ctatgtgatt gacgatgata tcgattttga
     3061 aagtaaacag cttttacgct tctcagtgaa aaacgcacga atgaactcag atgtggaatt
     3121 cttgaaaatc aataaagaat tctttacaaa tgtggtaatc agcgatcgta ttccagaaac
     3181 agcttattat cgtattgcga ttccggaatt gttccgcggc acagaagttg aacgtatctt
     3241 atatatggat tgcgacatga tcgctttaca agatatctct aaattatggc gtcttgactt
     3301 cggtgattca atcgtagcag ctgttgaaga cgctgggttc catcaacgac tggaaaaaat
     3361 gaaaatttca gctaaatcaa tgcgttattt caactcaggt ttgatgctga tcaatgtgaa
     3421 aaaatggtta gatgaaaata ttacgcaaaa agtattagat ttcatcgaac ataatccaga
     3481 aaaattacga ttccatgacc aagatgcatt aaatgctatc ttacatgacc gttggttacc
     3541 acttcatccg cgttggaatg cacaaggcta tatcatggca aaagcaaaaa aacatccaac
     3601 agcagcaggt gaacgtgaat acgaagaaac tcgcaacaat ccttacatta tccatttttc
     3661 aggacatgtg aaaccgtgga gcaaggattt tgaagggcca acgaagaaat attacgaaaa
     3721 atatgcaggg atgacggctt tccgttgtgt agctaaattc cctaaatatc caaaatatgc
     3781 aaaagttcaa ccaaaaacag atgtagttgt taaataatag aagaccacaa ctgaagaggg
     3841 ggagtaggaa tggaaattac aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt
     3901 ggggaaaata caaaaatcgc actcgcgctt atcaatgatt ccgatccatt tctaagggac
     3961 aaaggtgctt ttgcaaaagg cagctttttt caaatcattc cttttgtttc ggaatttgga
     4021 gaatatgcga ctaaaatcga gcatccatta ttggacatct atacatctaa attagagcaa
     4081 aattatttcg gtaaacgatt gaacatggac ttcaacaaac agcttgacag cttttcatta
     4141 gaaaatgaaa tcgcagtact cgattataat atcaaattaa agaactgctt cttcagctaa
     4201 tagtttcgag aaagtccaaa aaggaggtat acattgagat actactttgt ggatgaagaa
     4261 aagaatgtaa tagaaacaga tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag
     4321 cccgaagaga ttgagcaagc aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga
     4381 accgaatatc cggaagaagt ttctcggatg gagcgccttt atgagacgaa actagagcat
     4441 ccattttctt tggtagtgat taatctagat cagtcagata atcgtatcga aaaaaaatta
     4501 acgccaatat cttttgtact ttccgataat ctcttgatta cttgtatcga tagaaaaacc
     4561 gaatttgtgg atcggctgct gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc
     4621 atcacgtata cattattaga tatttacacc cattacgtga aagaactaag agagatgaaa
     4681 aagcgaattg acgctttgga taaagcagct cgtaaaacaa cggaaaatga agaactgtat
     4741 aaacaggcag atcttgaaag agacatcgta tatatcgatc ataccttaag agatcaacgg
     4801 gaaacaatgg atcaattatg gaattctgaa actttcatca aacgtttaga cgatgaacag
     4861 ctactctatg atgtccattt gagacagcga caaacagaaa aaatgatcga gatctatcga
     4921 gatttattag agagtattgg tgatctattc aacgggatga tggacaataa tctaaatcat
     4981 atcatgaaat atctagattc agctgcatta atcatttccg ttccggcgtt gttcgcaggg
     5041 atttggggaa tgaatacagg aggattaccg ggtaaaagtt ctgctctagg ctttatactc
     5101 gtagtctttg gctctttgat agcagcagta gttttaggtt accatttata ccggaaagat
     5161 tatacaaatt aaagaatcta tttaaaacag gtatcaaaca aagaaatttg ttttggcaat
     5221 acaatttaaa aactaggagg aatacagcaa tgacagaacc aaaattaatc gacccgcgaa
     5281 acttgtacca tacggagaaa tttcctaaac aagatcagga tacaccagct ttacagcaga
     5341 atatgattcc tgttcctgat tgtggagaag aatcctatga aggcaataat caactagaaa
     5401 accgccgtgt actaatcact ggtggagatt cagggatcgg tcgtgcagca gcgattgcat
     5461 ttgctagaga aggggcagat atcgctcttc aattcttccc aggagaagaa aaagatgcag
     5521 aggaagtagc aaaatacgtg aaagatgcag gacgtaagat tgtcttacta ccttttgatc
     5581 ttcgtgacga acaagcacct aaagaaatca tagataaagc cgtgagtgaa cttggcgggc
     5641 tagatacgat ggtactaaac gcaggacagc aaatttcttg tcagtctatt gctgagttac
     5701 cacttgagca agtcaaagac acatttatga taaacatcat cgctatgttt gctttagtga
     5761 aagaagctgt tccgcatatc ccagcaggag gagcaatcgt aacaacaact tctgttcaag
     5821 cattcaatcc aagcgaacat ttattagatt acgcagcaac aaaagcttca atcgctaact
     5881 ttacgatcgg tttagcaaaa cagcttgcac ctaaaggaat ccgtgtaaat ggtgtagcac
     5941 ctggtccaat ttggacacca ttacaactag acgaaggaca accttctgat tcgatccctg
     6001 aatttggtca gcaaagttta ctagaacgtg caggtcagcc aggagaatta gcaccagttt
     6061 atgttttctt agcatcgaat aaagcaagtt atgttacagc ccaaatttac ggagtaactg
     6121 gtggagaagc gatcaactta taagaagttg atcgtttcac ccctatgagg aggtgcgaaa
     6181 atgaataaag gtgtaaaaac gatcggagtg atcggaacat tacttttgct atctgtgctt
     6241 ttgtttgcga tactattaag tgcaacctat ccattgcctt ttcagctaga gaagtttcga
     6301 ttcttttcca tcactaattc atacgtgcaa caatatgtat tttggcttgc cgttgcattt
     6361 gctattttga caattatcac gttacttgtt ttcctgtttt atccaaaaac aatcggaaac
     6421 tttctactga aacatggtga tggggagttg acgttggata aaaaagcaat tgaaggaatg
     6481 gttcgttctc acttgcatga agatgaattc atccattcac caaaagtaaa tgtcaaagca
     6541 acgaaaaaca agatcgccgt caatgtaaaa ggggagttga aacgaacttc agcactcatt
     6601 ggtaaaacag gaacattgat gaaagaaatc gaaaatgaag tacaacacat attgggaaca
     6661 aaagagccag tgaaagtatc ggtgaaatac acaggctttg aagaaccagc taaacgaagc
     6721 aatgaccgca aacgagtaga ataggggggg accaaaaatg aaagaactat tttcgacata
     6781 caagctccct atcatctttg gggtacttgg attgatatta gccgttttat ggatcaccgt
     6841 gggctttttc aagaccttat tgatcttgat catggcagcg atcggcgtcg ctatcggttt
     6901 ttatctgaaa caaacgaaaa tcttagatca ctattttaaa tcttaggagg aaacatccat
     6961 gaataacaat aacaaaaata ataacaataa aatgaacaac caaaaaccag tgtctgcaca
     7021 tccagaccca acgacaccaa accaatcaaa tggtgtgaac gatgaaaaga acaaaacagc
     7081 accagtatct gcacatccag atccagtagt ccctaacgtt caacaaggag tagaagagaa
     7141 gaaacatgat gcagaaaagc atgcacctca tcatccagga actccagaat ctaaagttcc
     7201 agaacaacat gatgcaatcg atccttctaa acctgtggaa gagcaaaaac atccttctga
     7261 ccatgcgaaa gcacaagaaa aacttagttc tgaccacaca aacggacaag gaaaacttgg
     7321 ttctgatcat acaaataacc aaggaaagca tgattctgaa caggaagatt caatgcgcgg
     7381 tgagctgaat tatgaagata aagttgtgca aaaaatcatc ggaatcgcaa ttgaacaagt
     7441 agacggttta ttatctgcaa acggtggttt cttctctaat gtggcaggaa aacttgtgaa
     7501 tacagataat gtaacggctg ggatcgaaac agaagtcggt aaaaaacaag tagcagttga
     7561 tatggatgtc attgtggaat atggcaaaga tatcgaaaaa atctttgaag aaatgcaaga
     7621 agtaatcggc aaagaagtaa aaaatatgac acatttagaa gtgatcgaag tcaacgctaa
     7681 cgtagtggac atcaaaacaa aagaagaatt tgaaaaagat caagaaacgg tgcaagacaa
     7741 agtaactgac gcagcgaaaa aaaccggtga atttgcttcg aatcaaacag ataaagtaaa
     7801 atcagcagct ggaagcggcg cacaaaaagt aaaagaaaat acagaaccac gggttcagta
     7861 ataaggaagt aaaacattag gaggaacgaa acatgggatt tatttggtca cttatcgtcg
     7921 gaggaattat cggagcaatt gcaggagcaa tcacgaataa aggttcttcg atggggatta
     7981 ttgcaaatgt cgtagcaggt ttagttggat cagcacttgg tcaagcatta ttaggttctt
     8041 ggggaccaag tattgcagga atggcaatcg tgccatcatt gattggtgcc atcattttag
     8101 tactaatcgt atcattcttt gctcgaaaac tgtaaaaaga cttttaaaaa gaggtcgtga
     8161 aaaagctgtt cttcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt
     8221 catttggttg ggcatattat cgaagatgca agcttttgaa cactgtttat acggaataag
     8281 aggctgtgac aagacttttg tcacagcctc ttatttatgc gggcaaattc ccaattagat
     8341 ttttaaaagt ttttccttaa atgtgtcttt attgatacag agaagcttct ttaataaaac
     8401 aatgcctccc taaattttct tgagatagga aaggtgaaca tacaaaataa tacgaccact
     8461 ttgaaaaccc agatgatcca caaatggatc gtatgatact gatgaagtaa aaagcaatgg
     8521 atcagaaact caaagagatt gaaaaagaca aggaagaaat aatatctctt ttttactctt
     8581 gtctgtttta gccaataata gctcgtaaag taatctagac aaacaaatag aagtaaacaa
     8641 agtttcgtaa gagcagcaat cgtatcatgt tgctctgcta gacgattgac gagcaggaaa
     8701 aatgttggta atggaatata gatcacgagt aatgaaagaa aaaagtaaac aataaatggc
     8761 atactattcc tcctaattgt gtgagttcgc cctcatttta aaataacgat atttgtttgt
     8821 gaagcttttt c
//
LOCUS       DAZUKA010000115         8808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000115 DAZUKA010000000
VERSION     DAZUKA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.118"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..294
                     /locus_tag="ACG8PN_002218"
     CDS             <1..294
                     /locus_tag="ACG8PN_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862350.1"
                     /translation="YTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVR
                     TRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(391..825)
                     /locus_tag="ACG8PN_002219"
     CDS             complement(391..825)
                     /locus_tag="ACG8PN_002219"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862351.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1057..1767)
                     /locus_tag="ACG8PN_002220"
     CDS             complement(1057..1767)
                     /locus_tag="ACG8PN_002220"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4862352.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1757..2617)
                     /locus_tag="ACG8PN_002221"
     CDS             complement(1757..2617)
                     /locus_tag="ACG8PN_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4862353.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2622..3260)
                     /locus_tag="ACG8PN_002222"
     CDS             complement(2622..3260)
                     /locus_tag="ACG8PN_002222"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4862354.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3275..3574)
                     /locus_tag="ACG8PN_002223"
     CDS             complement(3275..3574)
                     /locus_tag="ACG8PN_002223"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4862355.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3617..5101)
                     /locus_tag="ACG8PN_002224"
     CDS             complement(3617..5101)
                     /locus_tag="ACG8PN_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4862356.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5121..5582)
                     /locus_tag="ACG8PN_002225"
     CDS             complement(5121..5582)
                     /locus_tag="ACG8PN_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4862357.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5600..6667)
                     /gene="ulaG"
                     /locus_tag="ACG8PN_002226"
     CDS             complement(5600..6667)
                     /gene="ulaG"
                     /locus_tag="ACG8PN_002226"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4862358.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6885..7655
                     /locus_tag="ACG8PN_002227"
     CDS             6885..7655
                     /locus_tag="ACG8PN_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4862359.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7928..8755)
                     /locus_tag="ACG8PN_002228"
     CDS             complement(7928..8755)
                     /locus_tag="ACG8PN_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862360.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2666 a         1858 c         1381 g         2903 t
ORIGIN      
        1 tatactgttg aatttgacag tgaactactt aagcaatttc cacaagaaga acatttcatg
       61 tataaagaag caatttccca caaagtgaca gagtatttgg agcataattt ttttcgtata
      121 aagccagtgc gtactcgttc ggcaaaagga atatacgaga tgaaagtcaa aattaataaa
      181 aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg tgttcatttt ttatattagt
      241 tcgaatttgc aaaaaattgt atttgataaa gaggtgagta aaaaaataaa atagctgagc
      301 attggaggat agattgagtg atactaagtc tatcctcttg tttttctaaa gaactaagct
      361 agaatttttt cttttgtacg ttattatatg ttatttgtca ttaccaaaaa gcaccttttt
      421 tcttctagta tctatgacat aagaaatacc acccatttcc tttacttgaa caaatcctac
      481 tgattcataa aatttttttg ttttctcagt atcatctgtc aataattcga tttggactgg
      541 tccataacta gtgatcttct ccaaagcttc attcaaaagc actttgccta ttccttggcg
      601 ctgatacctc ggatgaatga ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat
      661 ccaacgaacg attccgataa tcttcccttc cccattacgc tttatcagag attcagattg
      721 ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt
      781 atataattca gaaatctcat cgatcataag ggaatcaata ttcatttttt tcttcctttc
      841 ttatacaatt ctccctctaa taatacagtt ctttgactat ttaatctatc acatataact
      901 ctcctgtaaa gattctctcg attccttaaa attcctcttt ccttttagaa tgcatacttt
      961 ttgataaata aaaaggttgt gataaatttt cttcacaacc tctgtttctc tctatcaagt
     1021 gttatctttt aattttctac ccttacccta gcatctctat tgcccgtagt atgcatactt
     1081 tccatgtttg cgaaaataat gtttatctag taaatattgc gggatttcac atatattggg
     1141 gttgatcagc tcgttttctt tggccatcag acaaatttca tctaagatca aactgttttc
     1201 tactgctttc ataggtgaat ccccccatgt gaacggtcca tgtccataga caagtactcc
     1261 agggacttcg tttggatcta attttctttc cttaaacgtc tcgacaatca cattccctgt
     1321 atgaacttcg taagcttctc ttacttcttc tttcgttaat tggcgtgtac aaggaacctt
     1381 accataaaaa gcatcagcat gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc
     1441 ccacatgacg gagtgcgtgg aatgagtatg agtaatagca gttatatctt caaaagtttg
     1501 atacaaaaca acatgtgtgg ggagatctga agatggtttt aagctatctt cttctattat
     1561 atttcccgac aaatctgtta ccaccatttg atctgcttgc atgcattcat atctgacacc
     1621 gcttggctta atgacgatga ctcctagctc acggtccact tcactcacat tgccccaagt
     1681 caatttgact aatccgtatc gcggcaatgc cagatttgct tcaaggacac gttgcttcat
     1741 tgctgcaatt ttttcttcac aagacatcat agccagcctc cttcaattta gaacttaagt
     1801 agtcttttgc ctgctggatt tctcttcgaa agtcttggct tttttcactc cacatttcaa
     1861 tcagaaatgt tccatcataa tctagacgtt tcaaatgtct taataatcca agaaaatcaa
     1921 cacaaccgct accaaaagga acatcacgaa actggcctga atattctttt gttacagggt
     1981 aagtatcttt taagtggata gcagtgatat aatcgatccc tttctcaagt tctcgagccg
     2041 gattattttc cggccatgcg gacaaattgc ctaggtctgg atatacttgt agaaaaggtg
     2101 aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga gttcataaat ggatcatcca
     2161 tgatttccac ggaaagaatg atgccatatt tagaagcttc ctttacgctt tcttttattc
     2221 cttcgataaa gtcttctctg gaattcattg atttctcttc ataataaaca tcgtatccag
     2281 ctatttggat tactttgacg cttagattat gcgccaaatg gattgctttt ttcattagtt
     2341 gtttcgctgc ttttttcttt tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac
     2401 ttaaacaaat cgaatggata cgtacaccgg agctgaacat ctcttctctc agctcagcta
     2461 tcttttcttc agaccaatct aatcgtgcta accgctcatc cgtctcatca atcgacatct
     2521 cgataaaatc gaagtccatt tcttttacaa gggaaaaacg ttctttccat gaaatatctt
     2581 tgggtaacgc tttttcataa atcccaattt gagccatttc cttaccccca gatacgaatg
     2641 atctcatctt tgaagttttg tgcggccttc atcggatcat ttgctgctgt aataccacga
     2701 ccagtgataa aggtatacac atcgatccct tcaaataagt gtaatgtgtg tggatttaat
     2761 ccaccagtta cagaaacatt aaagcctagt tcaatcagtt ttttgatctt cgataaatct
     2821 ttttcacccc aagtttcacc agataacagc gcatcacggc tttgatggta gattacctgc
     2881 cggataccga tgttatgcca atccatggct tgttcaaaag tccaattgcc gtagagttct
     2941 acttgcaatt cacctatttc tttttgtgct gcctccatag ttggaatcgt tgcacaacaa
     3001 atgactgtca tccagtctgc tcctgctttt gcaacattac tcgccactgt tcctccagcg
     3061 tctgcacatt tcgtatcagc gactagtttt ttatctggga acatactgcg aatacagcga
     3121 atcgcttttt gtccttcctg taagcaaaga attgtgccca cttcaataat atctactaca
     3181 tcaccaactt tcataacatc tcctaatgca tgttctaagt cattgtgatc taacgcaatt
     3241 tgcaaattcg gcctgctcat cttagttcct tcctttactt gtttttttgg taccactttg
     3301 tttcagcaaa tttttcactg atttctttga ctgacatcac atttttgata ccgataactg
     3361 taactccttt tttctctgcc tgagtaaaca tattcaaaaa attgactggc gtaaaaacta
     3421 catcgtattg gccagctgaa ctttttccct ctgaaatggc acaatgatgg attttggtaa
     3481 ttggtacgtt tagttctttc attgcttttt ccacacttct catcatcatc aagcttgttc
     3541 ctgatccatt tgcacacgat actaaaattc tcatcttaat ccctccatta tgagtgttgt
     3601 ctaatcagat tttcttttat actctctgct tttctgcata tgtgtcatag tcttctacaa
     3661 tcaaaaaata accatcagga tttttccgat attgtaactg cggaatagcc aataaaccaa
     3721 tgacaactac ggcaattcca gcataaccta ggactttcat gattactgta aacaatggcc
     3781 aagctgttgc ccagtcgaac atcccgatat aaccaccgta tgttgacatc ccaatccatg
     3841 tagcgaataa tgctgaaccg cctacttgta tgattcctga aagaaacggg aacaggcaag
     3901 cggctttgaa tccacctcgg ttattggcat aaactgctat aacggcatta tcaaagaaca
     3961 acggaatgaa accggctatg acgatcgttg gtgatttcaa taaaatcagt aaaccaatca
     4021 tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa
     4081 agccaaaagt agcagctacg tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga
     4141 ttccttggaa agattgagtt agttcatcga cgaatgtccg aacacctaat tgcaagatcg
     4201 ccagatatac cgcaaaattt aaagaagtcg tcataatgta gaataagaaa ctctgccctt
     4261 cttgcatgaa ttgcgcttga atcaaataat ctttgcctag tacaatcaga atgatcccaa
     4321 agaaaaatag catgagtaac gaagtagcaa ccatattctc attgaaaatc gacaagaatc
     4381 ctggtaattt gatatcttca atttttttgt cttgcttgat tgatctattt ttcttttcat
     4441 cccgcttttt catccactca gctaattttg caaatataaa gattccgaac atttgctgat
     4501 gtgccactgc aaatccggca ccttctgtca attcctgcgt gattcctact gttaagttag
     4561 aagctactgt ccaataacaa cctaaaatca gtcccataaa aatcaatgct tgtatcctac
     4621 ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa
     4681 cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt ctgaaaacgg actaataaga
     4741 tatttaaaac aaatgcaatc agtaataaaa tcatcacatc tccaaaggta cggccaaatg
     4801 tctccattaa tccagcatcc acagcatttt gtccaaaata aggatctgta accatggcat
     4861 ccaaattgaa ccgttccttt agtccaacca agattggccg aaaattattg accaaaccac
     4921 cagagccaac agtcaaaata aagtaaccga ccgttgcctt taagaatcca gcgatgcatt
     4981 catgaagcgg gcgtttcagc aaaatatagc ctaataacac gataaaacca atcaggtatg
     5041 ctggctgcgt caagatattt gttgcaaaat acgtccaaat gcttaacaaa aattccccca
     5101 tcttttttct cctctatcaa ttaattatat tctgccatca caacttggta atcatcgatg
     5161 gagttcgtag agatgagacg ctcaaccaat ccatctgtca ttaataattc ggataatttt
     5221 tggatatttt ctagatgttc ttctggatct tttgctgcga gtgtaaaaaa caaactagcc
     5281 gttttctcct cattctcccc ttcactttca aaacggattg gctgtttcaa cttggtaaat
     5341 gaaatagctg ttccaaaaac accatcactt ttttctgaag aatgcggcat tgctacttga
     5401 ggtgcgatca caatataagg accgtatttt tctacacaat ggatgatctc ctccacatac
     5461 gtttcatcaa taatttcctt atcgatcaat acaagacagc tttcggcgat agcttcacgc
     5521 caatccgtga tttctttatc tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc
     5581 aagaaactcc ccttctttct tataaaaacg acggaaacgg tgtatcattt tcaatcgaga
     5641 agacatcttc aaatcctcga taaaaattaa actcaagatc atctttgttt gtcggataga
     5701 tgaatttccc gcctacttgc cagatataag gtttaaatcc atacttcagg cgatcctttt
     5761 tgaatttcca aatttcagtg atctcttttg gatcagccat aaaattagac caaatatcgt
     5821 agtggacagg aatcactact tttgcattca atgactccgc catgcgcagc atatcaacag
     5881 aagtaacttt gtctgttatc cctcgtgggt tttcaccata ggcccctaaa cagacatcga
     5941 ttttgtgttc attaccatgt ttggcaaata aattggagta atgtgaatct cctgcatgat
     6001 aaagattccc tcctgatgtt tcgaataaat agtttactgc aatttgatcc atctcctgag
     6061 gcatttttcc ttttaatacc tcttctggat cttcacaagt gaccaacgct gtccgatcaa
     6121 acgcttctaa agctactaca ttgatgtctt tgattttgac tgcatcacct ggctttactg
     6181 taatcgtttt ttcttcagga attccccatt tttgccacgt gcgaaccact tcttttggcc
     6241 caatgaatgt tgcttcagga caattttgat gaactgctgc ggctgtatgg atatctagat
     6301 gatcggaatg gatatgtgta acgattaaag cgtctacatt ttttatcgca aaaggatcga
     6361 ttacgaaagg ctgagtacgt aaattaggct gcatattttg aacgccactc atacgcatca
     6421 tctgatggcc ttttttcatc atgccattcc catgactacg ttttcccgtc ccacaccata
     6481 gatcacataa aatattcgtt ccttcatgac ttttcagcca aattcccgta cagcccagcc
     6541 accacatagc aaaagttcca ggatccacta cttctgtttc gatttcttca ttcaagtatg
     6601 tgccccactc aggaaatgtt tctactaacc acttttcttt tgttacttca ttgacagttt
     6661 tactcatttg cttgctcccc ccatatcgtt gttccaactt acaagtaaat attaatgtgt
     6721 atcagcatcc atgaaaaccc ttttatatga ccgtttgttt tttatatgat tttctttcag
     6781 taatcaatga ttgtttcagt ataataaaga cagatatgaa tgtttaaatg aacaactagt
     6841 caaataaatc catggatcaa gatgtccctc aggaggcaaa agtcatgaaa aactcacttg
     6901 tcaatattga aaaacgtcaa aaagaaattt tggaactgct tcagcagact gaacaactat
     6961 caactttcga attagctgaa tcccttgatg tatctattag tacgatacga agagatttaa
     7021 atttattaga agaaaaaaac gagatcatac gcaattacgg ttattgttct tataattaca
     7081 aaaaccagac tgattttgat caatctggtc ctgaaaggat taaacaagcg attgccagag
     7141 aagctagtaa atacattaac gattatgata cgctgtttat caattctagc tcgactgcgt
     7201 tgaaaacact ggattttctt caatctaacc atttgacagt cattacaaac aacgtgaaaa
     7261 tcaattactc tccgcacaaa gataattgta gttatatttt aactggtgga gagatgcgct
     7321 ttccaaaaga agcactagtg ggcgacatcg cattaaatac gatttcttca gtcaatgcag
     7381 acatttgtat cattggctgt agcggagtcg atgtagtaca tggggtcaca acaaagatat
     7441 taaatgaatc aacgatcaac gaaatgatgg tcaacaagac aatcaaatgt aagattttag
     7501 tcgctgatca tcgaaaaatc ggactgacat caaagttcaa gattgctgat atttcagtct
     7561 ttgattattt gatcactgat aaattttgtt ctgaaaaagt attagaggag atcagaaata
     7621 cggggataaa aaccattcaa gtagattcat tttaagaacc aggacgtaaa tatgtaaata
     7681 ggattatcaa aaaaatgaat aacaaattta tcgataaatg aaaaacacgc tacgctcaaa
     7741 ttgataaagg aatcccaaga tgtatccatc caatcgatac catcataatc tattgcttct
     7801 tttataaccg ttatatgttg cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg
     7861 acaaaagtca cacctccttt tttcgaataa acggtattca aatcagaaca ctgatttcac
     7921 aaccacttta tttcaggtcg ttttcaatca cctttaccgc gtaatcattt ccgttgaaac
     7981 gcgctatttt ttttgcgtcc atatatcttt ctaaataatc atcttctcct aattgcttat
     8041 ggcaagaagc aatttcataa agcaagtatt ccaatgcata agtcgattta tgtttcttat
     8101 tatactcaat acccttctca cataattgga ttgctttatc ataatcctcg attttacggt
     8161 aataggtggc gatatcataa tatacaatgc agaccatcag attatcaaaa tcagtctcac
     8221 tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa atctaattct ccttttgaca
     8281 agtagatcat tgccacgttt gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt
     8341 gttccaacac ttgattaaaa taaaataatg ctgcatctgg ttttttcgca taaagatgat
     8401 caagcagtcc ttgataaaat aaaatttgat ttaaaagatc agaattactt actttttcct
     8461 gatcgatcgt ttgtaaaatt ttataagatt cttggtattt tgatacagaa ttcaatgatt
     8521 gaatctcatt tatttttttc ctgattttgt cttcttctgt ttctactaat atatcggaga
     8581 tctcgacatt cagctttttg caaagcgcac ttaagatatt gatgcctggc gacatattct
     8641 tgttttcgat attgctgaca gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg
     8701 ttaacccctt tttctttcgg tattctttta gtaatgctcc atctacttta atcaaaacaa
     8761 tttccttccc tttaaaaaat tatttctcct ttatatcata cacaaaaa
//
LOCUS       DAZUKA010000116         8784 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000116 DAZUKA010000000
VERSION     DAZUKA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.119"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(382..2763)
                     /locus_tag="ACG8PN_002229"
     CDS             complement(382..2763)
                     /locus_tag="ACG8PN_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4862361.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2753..3400)
                     /gene="recU"
                     /locus_tag="ACG8PN_002230"
     CDS             complement(2753..3400)
                     /gene="recU"
                     /locus_tag="ACG8PN_002230"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4862362.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3464..4003
                     /locus_tag="ACG8PN_002231"
     CDS             3464..4003
                     /locus_tag="ACG8PN_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4862363.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4093..4512
                     /gene="gpsB"
                     /locus_tag="ACG8PN_002232"
     CDS             4093..4512
                     /gene="gpsB"
                     /locus_tag="ACG8PN_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4862364.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4534..4901
                     /gene="rnpB"
                     /locus_tag="ACG8PN_002233"
     ncRNA           4534..4901
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8PN_002233"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5110..6276
                     /locus_tag="ACG8PN_002234"
     CDS             5110..6276
                     /locus_tag="ACG8PN_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4862365.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6322..7818
                     /locus_tag="ACG8PN_002235"
     CDS             6322..7818
                     /locus_tag="ACG8PN_002235"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4862366.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7899..8582
                     /locus_tag="ACG8PN_002236"
     CDS             7899..8582
                     /locus_tag="ACG8PN_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4862367.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8591..>8784
                     /locus_tag="ACG8PN_002237"
     CDS             8591..>8784
                     /locus_tag="ACG8PN_002237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862368.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2774 a         1586 c         1855 g         2569 t
ORIGIN      
        1 gatggtgcag acaaattatg ataattaagc atactatcag ataactcaaa atgaagcctt
       61 ctttcaaaaa attattcaaa accatatatt gtatttttta tttgataata acaatgggat
      121 tattaagaag tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca
      181 agtaaatgga taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct
      241 aaatgttaga tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct
      301 tggtacatga tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt
      361 gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt
      421 atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc
      481 agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc
      541 tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg
      601 actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac
      661 atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg
      721 atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt
      781 atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg
      841 tgacgattct gaagtgacag gcgtcatttt tttattgtaa cctgtccaca cggaaatcga
      901 ataatttggc gtatagcccg caaataggat atctggatac accccagaag aaataccaag
      961 ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc
     1021 agcgatttgc gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat
     1081 catataagct gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt
     1141 tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc
     1201 gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg
     1261 ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag
     1321 attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg
     1381 aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc
     1441 ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt
     1501 tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt
     1561 agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc
     1621 gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga
     1681 ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata
     1741 tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac
     1801 attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat
     1861 cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac
     1921 agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt
     1981 atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg
     2041 aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa
     2101 gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac
     2161 gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc
     2221 ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt
     2281 gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag
     2341 ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc
     2401 taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg
     2461 cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac
     2521 ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag
     2581 tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat
     2641 aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt
     2701 cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc
     2761 cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat
     2821 aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata
     2881 aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac
     2941 tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat
     3001 tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa
     3061 aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc
     3121 cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc
     3181 tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca
     3241 acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc
     3301 gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat
     3361 tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc
     3421 cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt
     3481 aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa
     3541 aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct
     3601 ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc
     3661 ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt
     3721 tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga
     3781 ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac
     3841 tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg
     3901 aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat
     3961 tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat
     4021 ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact
     4081 agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc
     4141 aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc
     4201 aaagattatg aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg
     4261 agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa
     4321 acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat
     4381 cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca
     4441 caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca
     4501 cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg
     4561 atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag
     4621 cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg
     4681 ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg
     4741 tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct
     4801 ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt
     4861 ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa
     4921 ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt
     4981 tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg
     5041 gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg
     5101 agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta
     5161 gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga
     5221 cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca
     5281 gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt
     5341 gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta
     5401 gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa
     5461 aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg
     5521 gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact
     5581 ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca
     5641 ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt
     5701 ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc
     5761 cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt
     5821 caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa
     5881 ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca
     5941 gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa
     6001 acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa
     6061 gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca
     6121 tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca
     6181 accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga
     6241 actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt
     6301 ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc
     6361 aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa
     6421 aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa
     6481 aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg
     6541 tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc
     6601 gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg
     6661 caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt
     6721 gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt
     6781 ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag
     6841 cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa
     6901 acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta
     6961 atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt
     7021 atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc
     7081 aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc
     7141 gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca
     7201 caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat
     7261 cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat
     7321 cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac
     7381 cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat
     7441 gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca
     7501 acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat
     7561 tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa
     7621 gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg
     7681 tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca
     7741 acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat
     7801 gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag
     7861 atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg
     7921 ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca
     7981 aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg
     8041 tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga
     8101 acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga
     8161 tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg
     8221 ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag
     8281 caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc
     8341 ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga
     8401 tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat
     8461 atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg
     8521 aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat
     8581 gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag
     8641 ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca
     8701 aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga
     8761 attggcagga gaacaccgtt tatt
//
LOCUS       DAZUKA010000117         8781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000117 DAZUKA010000000
VERSION     DAZUKA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.120"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1112
                     /locus_tag="ACG8PN_002238"
     CDS             51..1112
                     /locus_tag="ACG8PN_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4862369.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1115..2011
                     /gene="murQ"
                     /locus_tag="ACG8PN_002239"
     CDS             1115..2011
                     /gene="murQ"
                     /locus_tag="ACG8PN_002239"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4862370.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2025..4007
                     /locus_tag="ACG8PN_002240"
     CDS             2025..4007
                     /locus_tag="ACG8PN_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4862371.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4072..4518)
                     /gene="nrdI"
                     /locus_tag="ACG8PN_002241"
     CDS             complement(4072..4518)
                     /gene="nrdI"
                     /locus_tag="ACG8PN_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4862372.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4729..6153
                     /locus_tag="ACG8PN_002242"
     CDS             4729..6153
                     /locus_tag="ACG8PN_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4862373.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6334..7107)
                     /locus_tag="ACG8PN_002243"
     CDS             complement(6334..7107)
                     /locus_tag="ACG8PN_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4862374.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7104..8009)
                     /locus_tag="ACG8PN_002244"
     CDS             complement(7104..8009)
                     /locus_tag="ACG8PN_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862375.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8163..8483)
                     /locus_tag="ACG8PN_002245"
     CDS             complement(8163..8483)
                     /locus_tag="ACG8PN_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4862376.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2757 a         1580 c         1871 g         2573 t
ORIGIN      
        1 atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct
       61 tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg
      121 catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat gatgtttcgg
      181 catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagaattga
      241 tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa gatctaacgc
      301 ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc aatcagcaga
      361 ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag
      421 atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct tggcataatt
      481 attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa aatcaatggc
      541 tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag ttgagaggcc
      601 cactatatca aggattgcca actttggaag aacatcgagg gatccatcct cttgcaggcg
      661 ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca ggtctgtcag
      721 aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg
      781 aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat cggcaagacg
      841 atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg cctatcccag
      901 caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa aagtatttgc
      961 gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg
     1021 tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa gctggtatga
     1081 actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct
     1141 aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta tttctcaagc
     1201 cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa aagaactgac
     1261 aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat
     1321 ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg aatgcgtacc
     1381 gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag aagaagcaat
     1441 gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag atctaatcaa
     1501 gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata
     1561 tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg
     1621 caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc
     1681 agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa
     1741 tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact tgatggtcga
     1801 tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca tgcaagcgac
     1861 agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct
     1921 ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa aattagctga
     1981 agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca aaaaacgaac
     2041 tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg ggcaatgtag
     2101 aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat gatcttgtaa
     2161 atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat gaacagcttc
     2221 aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt gatttggcag
     2281 gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat tcatcagcat
     2341 ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac
     2401 aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt gtgccaatga
     2461 ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta gcaaacttag
     2521 tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg atgaacatct
     2581 tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct gcacaagttt
     2641 ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg acagggatga
     2701 atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct ggacaaggtg
     2761 ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa aaactgcata
     2821 aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg attgccatcg
     2881 gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat ggcatggtcg
     2941 gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat
     3001 tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc catgtgcaaa
     3061 tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct ggtgcaggac
     3121 aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa ttaacagaaa
     3181 tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg atttacggag
     3241 taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt atcggcgggg
     3301 cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca ggagccgctt
     3361 tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta ttggcagctt
     3421 atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt ttagaaaaag
     3481 aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta gtacctcaaa
     3541 cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt gtttctttag
     3601 ataaagcaag tgatccagtt tttgcacaaa agatgatggg tgaaggctac tttgtagaac
     3661 cagaaaacgg acatatctat tctccagtta ctggggtagt cagctctgtc ttcccaacga
     3721 aacacgctat tgggatcaca acgccaaatg gtttagaaat cttgcttcat atgggaatca
     3781 acacagttga cttaggcggt acgccatttg atgtgaaagt aacagaaggg cagcaagtga
     3841 cacctgatac gcttgtagca gaagctgact tagaagcgat caaagcagca ggcaaagaaa
     3901 catccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc ttggaaaaaa
     3961 caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat gatagaaaca
     4021 atagaaacga aagaagaaac tgaaatctat ttttcagctt cttctttttt attatttatt
     4081 gttttctgct aaaatctgat aaattcgtgt cacatcagat ggtgttcccc gtaattcata
     4141 atctgccaag aaaggaaagc caaatgtttg tgcatattgt ttggctgtta gacaatactg
     4201 attattgaag tttttatttc cgctaccgac tacaccaaga caaagtttat gattgtcttc
     4261 atgttccaga tattcacgca tcgtttcggt caagatttct gtatcaccgt tatctaaacc
     4321 atttccgccg tctaaataag taggaacgaa agtaaaaaat ggttctgttt ctttcgtaaa
     4381 atcactattt tcatggattt cttttaaagt aacttcagga ttgacaggat cttctgcatg
     4441 cattttttca gcatattctg ctagatgttt ggcaaaggca cgcgtatttc ctgaaatcga
     4501 tatatataaa atgttcatgg atcatcctcc aattctttcc ttttagtata gcatcttttt
     4561 taagtttttt ggtggtgtaa tttggaaagg gactaggcgc tttgcttgta taatgagaaa
     4621 aaagccggta gaattatatt tctattaaag tttttattaa ttaagtaaaa ataaaaaagg
     4681 tttgttatac tttttgataa aagcggtttt attttggagg atgcttacat ggatacagag
     4741 caacagaaaa aaggaattgg tttaattcca cttgctgcct tagtaattgg ttcagcgatt
     4801 ggaggtggcg tatttggtat tatgacggac atctccggat cggctggcgg cccagctatt
     4861 ttcagttggg tactcgtagg aacagcaatg ttgatgctct ccttgtcgat caataatatc
     4921 aataaaaaac gtccagattt aaaaggcggg atctttagtt acgcagaaga aggttttggc
     4981 cgtttcgctg gttttatcag cggttggggt tactggctca catgctggtt aggcaacgta
     5041 gcttttgcaa cattgttgat gagcgcatta ggatatttct tcccagtatt tgaaggtggg
     5101 caaaatctgg catcgatcgt gatgagtaca gtctttttat ggggatatgc atggctagtg
     5161 aatcggggag tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg taaattagtg
     5221 ccattatttt tatttattgt agtagctatc agcacgtttt cgatccaaca attcattgat
     5281 aacttttcta gcgacattat attgaatgcg aatggacagc cgatcccgtt ttccagtcaa
     5341 gtattgagtt gcatcatggt gatgctttgg gtatttgttg gtattgaagg agcctctgtc
     5401 gtaggttcac gagcaaaaaa acaatcagat gttgggaaag caacgattct agggatattc
     5461 ggcttgatta ttatttacgt cttggtttcc atgcttccat atgggacaat gacgatgaat
     5521 cagctgcaaa cgatcaaggc gcagcctgct atgggctatg tgtttgaaat gatggttggt
     5581 aaatggggag cagtcgtgat caacggcggt ttgatcattt ctttagttgg tgtttggctc
     5641 tcatggacga tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg acttttgcct
     5701 aaagcatggg gaaaactcaa caaaaatggg gcaccaactt atgctatcgt attgacgacg
     5761 ttatgtacga atgtcttttt actttcatta ttattcactg acagtgctta taactttgct
     5821 tacacacttg gtacagcagc tattttcttc acgtggcttt tcttaggatt gtaccaaatg
     5881 aaactttctt atcagcggaa agaatgggtc caatttttag tagggctttt agcaagtgcc
     5941 tttcaagtat gggctatgct agtcgtagcc tttaatgaag taatgcttgt tcttgttctt
     6001 ttcttgccag gcatttattt ttacctcaaa gcacgtaagg agcaaaacga gcctgtacaa
     6061 catatccact tagataagat tttacttata ggtgtgacga tggtcggagt gattgcaatg
     6121 gttttatttg ccactgggat gcttcacgta tagaagtata aaagaagtat aaagagaggt
     6181 tgtgagcctg tcgtttattc aaaaacatac taggaaagga gctgtgacac aaatgttgtc
     6241 acagctcctt tttcagaata aacggtgttt aagagacagc caatcggcaa taaggctgaa
     6301 cgtctctttc acagcctctt ttttgatgag tcatcataca ttttttcgct taaatacgag
     6361 atatcctaat gctaagaaca aaacgatgta agctaaatta ccgaatagca tttgattagt
     6421 agaaagatga gtcaattgct tcatgatctc ttcgtttcca atttggtttt gcagattcaa
     6481 catactgatc ggattccatt tgatccactc ccatttttgg atgagtgcaa taaggatacc
     6541 agatatgatt gacgaagcaa aataaaagac gattcctgct gagatggatg cgccggaact
     6601 attgataaag caagcaatca tcaacactaa acttaaaatc agccataaac cgatataact
     6661 acccaaagta taagtgaaca agctttggat cagtgtttga ccagtagaaa ccttctccgt
     6721 gaaggaaatc gaagggaaaa gaatcagttt catcaacaca gtgacaataa tagctaaaag
     6781 gtataaataa acagaataaa taactaatgt gatccatttg cttactaata tttctcctcg
     6841 agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgcttcca tcgaaatgat
     6901 cgtgcttgca gctgcaatca tgataaagac aatccaagat gtggcactaa aaagctgagc
     6961 agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg ctgttcctat
     7021 gagcaaaaca actaatagaa cagataaaac agcagttgac tttttcttga tcaatttaaa
     7081 taattcttgt tttacaagca atgtcatttt tcttcttccc cctttactga atccagaatt
     7141 tccaatagag aagattccaa atcattcgtc agatgctgga cgtctgtcac atggatattt
     7201 tgtgcagaca atagctggat cacggttcct aataattctt cgttttcttt taaaagcttg
     7261 attttatcag gatcttccaa agtatatcca gcttctgtca agatcgttcg agcagctaga
     7321 tcatttgttg tagagataac gatgaaatct tgcgctgttt ggttcaattc agccattgta
     7381 cattgtttga tgatctttcc ttgattgatc aggatcagct catcgaccat tttttcaagc
     7441 tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa gtcggtaatg
     7501 atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc atctaaaata
     7561 acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt cattcccaac
     7621 gagtaggatt tcgctttttt gtgaatgtac ttttccatct tgagctgatt cacgatagtg
     7681 tcgatagcag atacatcgtt ggtttcagag aataacttca gatgatcata gcctgttaag
     7741 aatggataga tacccgggta ttcgatcaat gctcctactt gttccaaccc tttatgacta
     7801 gagggagaaa tctcattttc accaatacag attgttccat ctgagtaatg gatcagtcct
     7861 aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa tccaacgatt
     7921 ttgccagggg ataagctaaa actgacatcg ctgagggctt gtttgtgacc aaagttcttg
     7981 gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat agatagtaca
     8041 agcatacacc tgatgagtgg agaagtcgct aggtcattgg acttatttct tattttttca
     8101 taaaaaaatt gaatattaaa taaaaaggct tattttttct gatcagtact tattccaagt
     8161 gtctagtggc cgctagtaat cttgatgcca atgatactga tgagtagcat ccccacaaag
     8221 aaccaagtgg aaagattcag cttatcatga aacaagacca caccaattat gaccgaacca
     8281 actgcgccaa tccctgtcca gataggataa gagagactca tcggcaaact tttgattgcc
     8341 tttgacaaaa agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga ataattgatt
     8401 ttagaaaaac catcactcca tttcatcatc gttgaccaga atacttccaa aattcctgct
     8461 atgattaatt ccatccatgc catacttaca ttcctcctta actataaaaa aatacacaaa
     8521 aagaggaaaa tcatcccttc aaagacctaa tggaatgatc atcctcttta tcctacccgg
     8581 tggcagattg tatatctttt tctgtagtat agcacaccta tccagatatc ggcaatctca
     8641 tactttttag taaagtgtac caagcactga cggatatatc gttagcggaa aagaagatga
     8701 atgctttgca atttcataga aagaagttgt tatttattaa tcatttaaaa aaaacagtaa
     8761 ctagacctta tttctatata t
//
LOCUS       DAZUKA010000118         8756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000118 DAZUKA010000000
VERSION     DAZUKA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.121"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(382..2017)
                     /locus_tag="ACG8PN_002246"
                     /pseudo
     CDS             complement(382..2017)
                     /locus_tag="ACG8PN_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2456..3325)
                     /gene="scrK"
                     /locus_tag="ACG8PN_002247"
     CDS             complement(2456..3325)
                     /gene="scrK"
                     /locus_tag="ACG8PN_002247"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4862377.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3345..4466)
                     /locus_tag="ACG8PN_002248"
     CDS             complement(3345..4466)
                     /locus_tag="ACG8PN_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4862378.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4468..4863)
                     /locus_tag="ACG8PN_002249"
     CDS             complement(4468..4863)
                     /locus_tag="ACG8PN_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4862379.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4882..5679)
                     /locus_tag="ACG8PN_002250"
     CDS             complement(4882..5679)
                     /locus_tag="ACG8PN_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4862380.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5681..6469)
                     /locus_tag="ACG8PN_002251"
     CDS             complement(5681..6469)
                     /locus_tag="ACG8PN_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4862381.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6483..6959)
                     /locus_tag="ACG8PN_002252"
     CDS             complement(6483..6959)
                     /locus_tag="ACG8PN_002252"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4862382.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7031..>8756)
                     /locus_tag="ACG8PN_002253"
     CDS             complement(7031..>8756)
                     /locus_tag="ACG8PN_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4862383.1"
                     /translation="LNYRFSENVGNLKNIIQLTCANAFYFLDDLEELYIQMYHLPQWV
                     NFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEYLYQTWKKDTDKQQLFEKYDRY
                     FRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQIEFDYYYLQITSIFLNSWIYQI
                     QLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQ
                     QKRLALILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTG
                     YEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQ
                     VKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKRILEDSFPRDTDLVIETCDYYS
                     LVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNIRKFSNQLNRHFNDN
                     ELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLY
                     VHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKY
                     VYDYVKRA"
BASE COUNT         2837 a         1744 c         1262 g         2913 t
ORIGIN      
        1 ttagaagcat gtaatttttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa
       61 tcatcactta cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attgctatct
      121 agtgtacctt catttctttg tgcttaaatt caaagataat tttctcaata aagatttttg
      181 attccagtaa gattccttta ctccttttac aacatagcgt tgtcctttgt tttgaaacat
      241 cttcttcccc tctttgccat taaaaatagc ccaattctct ataaagaaga ttaagcttca
      301 ttattataat ataaagttta aatttggttt attacttact ttttcttatt ctctagtaag
      361 ctaatattga aaaaaatgta atcagatcat tatttttata tgttgtaact tcctgataag
      421 aaaactaatt aaaagaaaga ggtaactaca taaactaatt aaaaaagcta acttcatagt
      481 tggaggataa atggaagaaa cttcaatgat tgtttctcct tgtgttcctt caatcgacac
      541 tagtcctatt atcaatctct cttttttgtc catttacttt tgcgactgtt ccccaataat
      601 caaatacagg aagcgttatt ttttgtcctt tagattcatg tactgtatag actattttcg
      661 aatccatcac ttgataaggc atagtatgcc attccttgcc aattcttact tcatgattct
      721 gcaaatgttc tttgtattgt aatgcttccg tgggtgcata gtcataaagt cctccgggaa
      781 ggagtgtcgt gtaacgaata agattttctt gattcattac caagtgatct ctctgagtga
      841 caactttatg ttcatagttt gcttttgttg cagcagatac agccaacagt aataaaacaa
      901 ttgccgttat attaactact tttctttgct tagctgatgt gctcttcatg aaaacgaacg
      961 ataaaatcaa cgagccaaac agtacttgaa agcctaagat tcgccaagga aattgaatca
     1021 tttgaatggg tgtattttgt aataaagacc atggaaaaaa tttggtggat aagagccaag
     1081 agactaccgc actaaggtaa atcacttttt ctttgcgatt aaatccttta aaaaagaaga
     1141 gtggacagat caaagaaata ataccaatga ttcctaccgt aaaggttgtc aggtcattat
     1201 tcatcgcacc aatcaaacta tccgagacat ttaatgcttg gtgttgtaag gtcgttttat
     1261 ctggctcatt aatcgattga accatgattt gtcggaacat cggataccaa aagtatgctg
     1321 ttaaaagaat cgtcattcct actgcctttg acaactccac aatcaaccgg atgctttctt
     1381 tttttcttgt ttcacttact acaaagccgc ctaagactag taagccaacg gttattacac
     1441 tcataaaagt ggaaagaaca tgtgtataaa tgagtaagct catactgact gctaatggaa
     1501 ttgcacatgg ctttttctca taaatcagtg cgtaagcata atacataact atcggtataa
     1561 ccgtaagtgc aatcgcttca gcaatagcag aacgatgata aatatcaatc gtacggtacc
     1621 cataaaatgt atataaacag ctaaatataa aagccgcatc gattcttttt aaaaatttta
     1681 gtccataatg aaacatcagc atgatagtac aaacattcaa tagccaaaca tataagatat
     1741 aactaacgat taaattatta cttatccaat aaaaaatgac tgccggaaaa aaggtcaagt
     1801 agggataaaa ataattcact ccgctcccat aatgattaaa cgtcgtaaaa ttgattggac
     1861 cagaaaaaat tgaagataac cctttgactc tagcaaaatg gaaggcaata tcccccgatg
     1921 tagaaatagt atgaacattt ctaaaataat tgattgaata aagtccagca ataattgcta
     1981 ggagaatgga caatattatt tttttcttta cttgcattac ttaattatat actcctttca
     2041 tggattgctt tatctagtat aacttaaatt ttaaataaat cgttatttat ttttactctc
     2101 acttaaaaaa gcactaaaat tataagagag ggaggtaatg gtctgaacac caagaattgt
     2161 tccaacatcg aattgcttaa ctaatgagtt caaagcttaa ttatgtggct cttatattta
     2221 ccgagcgtgg attgcgatct ctttagcgaa acaaacatct ttcataaaaa aaacagccat
     2281 atccgctttc ttggatatga ctatttacta ctactttact atctttcatt cttcttgtct
     2341 cacttatgat aaggcgctgg gtatcatgag tgaatgatat tgctgaatac aaatagtccg
     2401 accacattaa gttgatcgga cttcataagc attttattga ctaatacttt tttgcttaag
     2461 aattgattct aacatctttt gctaaagtta agcatcctat agttcctgca ttatctgcta
     2521 acttaggtgt gacaatgtat tcttcgatat ttggcgtatc gacatagccg ttaattagtt
     2581 ctaaaaaacg tctttgtact ttctttttca gatgttcttg ttgcatgaca ccaccaccaa
     2641 aaattataat atctggtgaa agcatcaggg tcgtattaca ggcacattgt gcaatataat
     2701 ccgcctcgat ttcccagaac ggatcttcta tagaaagatc ttgtcccttg gttcccgtac
     2761 gcatctcgat agcgggtcca gatgccaacc cttcgagaca atcatgatga aaaggacaag
     2821 taccagagaa agtatctcct gaatgtctct taactaacgt atgccccatt tctggatggc
     2881 taaacccttc aacaaaaatc ccatcttgta tagctcctcc accaattcca gttccaacag
     2941 tgtaataaac aacactggat aaaccttttc catgacctga aacatactcc ccgtaacaag
     3001 cagcattcac atcggttgtc caggcaattg gaatttcgaa atgcttattc aacgttccaa
     3061 caaaatcaaa atttttccag gcagtttttg gagtagaggt gatatgtcca taggtctttg
     3121 attcacgatg gatatcaatc ggtccaaatg accctactcc aatgctttct aattgttctt
     3181 tatagtgatt aaagaaatca attaccaaac tcattgtttc attcggtgta gttgtaggaa
     3241 aactcactct ttctaaaacc tttaaatcat cattgccaat ggcacaaaca aatttagtcc
     3301 cacctgcttc tattgagcca tacatttctt actccaccgt ccttttatct aggaattcgt
     3361 tcttccctgt ctaatagttt ttcgaaaaac tcatgtggtt ccttttggta ccaataggca
     3421 acagaagaaa tatcgtcttg acgctcataa agcttgatgt catcatttcc tagggtttgg
     3481 aaagtaacac gtaaattttc tttgaaacta atggggtcgg gtaaatgcca gcggtacaac
     3541 ccatgcatcg gtaaagcatc atcgccaaaa ccatgaactg gatttggatt aggttttcca
     3601 gcactaaaat aatcccgggt gtgatctctt ttagattgaa aaggatagcc cataaatagc
     3661 gtattaaaat tttgagcata tgccctttct ccaaagttct ttttatggaa agcccaagcg
     3721 ccgccaaaat aatcttctgc tcctgtagat gttattgtag ggaaatcttc atctccatct
     3781 atataaaact taaattctcc ttccccccac cagtatcttt ccaatgcgca caaagcaaag
     3841 taggttccta cataataccc agtgcctttg attccatcaa taagggtgta atcttttcct
     3901 tttgtcgtga tgggttcacg gttccattta gcatgaaaat acatcgcatc atccggtact
     3961 tcagtcaatg cataattaaa tgtataaaaa atgtgcttaa taaactcagg atgttcattc
     4021 gttaaagtaa tcttagcttt ttttcgaaat ggcatctcaa aatagcaatt catccctgat
     4081 gttggattca ctacgattgg taaggaattc acttcgcatc ttgtcccaaa cccattacaa
     4141 aaaaaatctc ctaatggact ttctacagaa ggagtggatt catcatccca ataaatacgc
     4201 aaaattaaat cacgtaagac gaaactccca gcgattgttt tgtctgtcac tgtcatccaa
     4261 aaatgacgta aaattccagg gccttccatt tctgctaaag taattgtctc tccagcattt
     4321 atcggaacag aaggtgaccc ttttctccca attcctagat gactcgctgt cattgccgct
     4381 ttcccttttt caccattttt attttctggt gaaatagttc tactttcctc tcccttgtgg
     4441 agccatatat tatttaaaca attcatttta ttcgtcctct atcctttctt cacttttttg
     4501 attattcata tagatgattt gttgttgtgc ctccgcaata attgctccaa tatctaaatt
     4561 atcatttcct aactgcaaag cgattgacat tgccaaggga agattcattc ctgcaattac
     4621 gtgcactctg tcagaaatat aggtaaaaaa cttttgattt acacttcctc cagccatgtc
     4681 agttaaaaca attatttcgt ctgtagtatc cactaactct ttaatttgtt catctatatc
     4741 atcaatttct tcgacatatg cacataagct actaatttga ttatgcatac ctgttaaaaa
     4801 ctctagtgtc tctttaaaac cctcagctag tattgaatgg ctagcaatga tcaatcttct
     4861 cataattcaa atccttttcc tttacgctaa tattccaaat gcagccataa ccatcgcaaa
     4921 aacaataacg agtagaatca aaatggtcat actgattttt ttcttactga gtagataata
     4981 tgctgcagca gtaataatta ctggaattaa tgttggtaaa attttgtcca acattggttg
     5041 gattttcata ctaacttcac caaaagtgaa tttaagtggc gttgaaacac tgataactga
     5101 agcaattaat gatccaacta ctgttagccc caatactgaa gcagcttcag ttaaataatt
     5161 aatcttttca ccgtattcag taacaatctt cattccctgc ttataaccaa attcaaagcc
     5221 tctcattcgg aaaaagaaga ttaccatgat taaaatgaac cataaaaaga ttcctacagg
     5281 attattttct aaagccatat aagccgcaat tgatcccatg atggttggga tcatcgtcat
     5341 caaaagagag tccccaatac cagcaattgg acccatcgta ccaactttaa agtcattaat
     5401 cgcatctttt gcttctaatc cttgcttttc ctccattgca acacaagcac ctaaaataat
     5461 ggctgccagc caaggctgtg tattgtaata ctgaaagtga ttatttaaag cttcgatgta
     5521 ttcatcatca tctttgtata attttcttaa aataggtgac aatgcgtaag tcacagatgc
     5581 accttgctgt gtttgataat taaaggtaca aactgacatc aaccaacgat agttcgcttt
     5641 cctaagatcc tttttcgtta cttgatattt ttcgctcatt ttattcatcc tccccaataa
     5701 acccaacatc agaagctccc gcagaagcag tgatttgtgc atctttttca gctctatgat
     5761 acacattaaa tgctaaagcg aatcctatga tcgccgctcc aataataggt accttcaaaa
     5821 aagcggctaa gaaaaatcca acaagtaaat aactaatgta tcgacctgta ggcataaagc
     5881 gtaacaacat cgcaatacca acagcaggca acatttttcc ggctaatgtt agcccatcca
     5941 taaaccaatt aggtaaaaca tccaaaacgt aattaacagc agactgtcct aaagtaacgg
     6001 caataaatac tggaattgct gcagataaac caaagaaaat tggccccaac cacaaaacac
     6061 tattcatttt cttaaattct cgtttattcg catattcctg tgatttgcga acaacaaaac
     6121 catttaagat tttgacaatt acatctagtt gcacccctaa catacctaca ggtagtccta
     6181 ttgctaatcc ggcttctact ccaagctggc taccaaatgc cgttgcaata attgcgcacg
     6241 ttccatagtc cggtacagat gagccaccaa aaccggcaac tcctaatgac atcaattgaa
     6301 ctgtaccgcc aactatcaaa cctgttttta tatcacccat caaaattcct gtgaataatc
     6361 cccaaaaaac tgcgtaattt acaaagatca tcggaatccc ataataatca acatgtttaa
     6421 taaatgctaa tatagtcagc ataataattt gaaaaatagt aaaatgcatt atccttactc
     6481 ctttatccta attttgattg aatttcctgt gccttatccg ccggaacaaa ttgtgtaaca
     6541 atttctacac cagaatccaa caatttttta tacgtgctga tattttcatc tgatgcatag
     6601 gctctagaag atagctgtaa tgcattatct ttaacaacag taccgccaac aattagtcgg
     6661 tcaattctaa taccgttatc tactaaatga aggattgttt ccggctcttt cacgatgaca
     6721 aatacttttt cctgatcgta tttccccttt ttaaaattcg caatcgctgt ttcttcagtg
     6781 ataattgata cagccattcc agcaggtcta gctaaacgca tacttgattt ttggatgtcg
     6841 ttatttgcga cagaatcatc gatcaccatc actctgtttg ctttagacat tggtgcccat
     6901 tgcgtgacaa taatcccatg caataaacgg ttatcaactc tcgacataac aatactcata
     6961 aatacttcct cctttatctt tcattttctt acaagcaatt accatgccaa aacaaaaaga
     7021 acttgataaa tcaagctctt tttacataat cataaacgta tttaacctca tgaatcggga
     7081 tgtcgacact ataataatgt tcaacgacac tgaaagattg cttaaaatgt gtctgaaatt
     7141 tttgaaattc ctcactcgtt tcattgaaat aaatttcatc ttccaaagta ttttgtttta
     7201 ctaatcgttc aattaagcaa ctaatgtgaa catataatcc gaaacacgta ttattatcaa
     7261 atgaaattcc tagatcattt tgtaagatgt atatagcatc tgaaacccgt tctaataatt
     7321 tagtcggatt taatatggtc aattgctcca ttaaattcgt tagactaaaa ttcttcagaa
     7381 tattattgtt caaaactttc agttcattat cattaaagtg tctattcaat tgattggaga
     7441 attttcttat attcattcct aaaatcatat cttcaattga aaagaattct acatcttcta
     7501 gatgtggatt tagtgttcca acaataaaaa gaatattgtt ctctttacga agctccgata
     7561 aatagttttc attcactaaa gagtaatagt cacacgtttc tatgacaaga tcagtatccc
     7621 ttggaaagct atcttcaagt atccttttta atttaaacga agttcctaat ccgcttgcac
     7681 aagaacagac aatcatatcc ttcttaatat ttgatgattc ataaatataa tccacatttt
     7741 gattttcttt tacttgttca agaatctgtt taagcggtaa atctaataat atctgatttg
     7801 caacatcaat agccattctt gttgaaacat tgtttagtag tccgaatttt ttttgagcgt
     7861 ctagttccaa atttagataa attttctcca gtgaacccat atcaacaagt aaaataattt
     7921 cttcatatcc ggtacactct tgtaagtatc tattaatttt gtctgcaatc tcactacttg
     7981 aagtcattag tggcatatca atggcgtcgt atatatatct ctcaacgatc ctattagcag
     8041 tacttgctat acaggttgcc gttagatctc catgagccaa aatcagtgct aaacgttttt
     8101 gctgaaattc tttactacac attaacttaa ataaaataac caaaacattt ttttgaattt
     8161 ttgttaaata aatatcccct atttgaataa aacgattgac taattgaact tctcgactac
     8221 accaactatt aatatcattg atgaaggaat ataatttatc gtccgatgtt aattggattt
     8281 gataaatcca agaattcaaa aagatagacg ttatttgaag ataataatag tcaaattcta
     8341 tttgaaaatc tctctctatt tccttaacat actgtctaaa tcgaatctct tgataggtgg
     8401 gatatgttaa agctgaatta ttttggatat caacattctt ttgtctaaaa tatctatcat
     8461 atttctcaaa aagctgctgc ttatcagtgt ctttcttcca cgtttgataa agatactcta
     8521 taagttcgtt gatattaaaa acttcgtcta ctttttccaa atcatccatt tgaatatatt
     8581 ttaattcatt ctgtcttttc ataaccttca tgccgttgaa attcacccat tgtggtaaat
     8641 gatacatctg aatataaagt tcttccaaat catctaagaa ataaaaggca ttggcacagg
     8701 tcaattgaat gatatttttc aaattgccaa cattttcact aaagcgatag tttaat
//
LOCUS       DAZUKA010000119         8664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000119 DAZUKA010000000
VERSION     DAZUKA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.122"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..598
                     /locus_tag="ACG8PN_002254"
     CDS             <1..598
                     /locus_tag="ACG8PN_002254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4862384.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            579..1025
                     /locus_tag="ACG8PN_002255"
     CDS             579..1025
                     /locus_tag="ACG8PN_002255"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4862385.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1368..1691
                     /gene="relB"
                     /locus_tag="ACG8PN_002256"
     CDS             1368..1691
                     /gene="relB"
                     /locus_tag="ACG8PN_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4862386.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2011..2238
                     /locus_tag="ACG8PN_002257"
     CDS             2011..2238
                     /locus_tag="ACG8PN_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4862387.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2254..3420
                     /gene="hemW"
                     /locus_tag="ACG8PN_002258"
     CDS             2254..3420
                     /gene="hemW"
                     /locus_tag="ACG8PN_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4862388.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3655..4698
                     /gene="hrcA"
                     /locus_tag="ACG8PN_002259"
     CDS             3655..4698
                     /gene="hrcA"
                     /locus_tag="ACG8PN_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4862389.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4792..5355
                     /gene="grpE"
                     /locus_tag="ACG8PN_002260"
     CDS             4792..5355
                     /gene="grpE"
                     /locus_tag="ACG8PN_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4862390.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5406..7235
                     /gene="dnaK"
                     /locus_tag="ACG8PN_002261"
     CDS             5406..7235
                     /gene="dnaK"
                     /locus_tag="ACG8PN_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4862391.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7386..8552
                     /gene="dnaJ"
                     /locus_tag="ACG8PN_002262"
     CDS             7386..8552
                     /gene="dnaJ"
                     /locus_tag="ACG8PN_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4862392.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3035 a         1412 c         1868 g         2349 t
ORIGIN      
        1 tatgaatcca acattgatta ctaagaaaga attactaaaa aaattagata tttctactgg
       61 tgtattagct aatttaatta gaaatggcat gccaaaagag ggagaaatgt ttaatcttga
      121 taagattatt acatggcgag aaaattggtc taaaaacata ttaggtgagt tagaagttgg
      181 tagagtatat actaacaaag aaatttcaga gaagtttaaa tgttccaaac agggtggaat
      241 gagaagatct catcaaacta atactcttgt actgtttagt gatcaaacag gaagtaatgt
      301 ttataaagat aagtggttaa atggtatttt acaatatacc ggtatggggc taaaagggga
      361 tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta attttgtaaa
      421 gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag aagtatattt
      481 agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca gaaaagtata
      541 taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag atatataacc
      601 aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta gaggaagaag
      661 caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac gaagaggagt
      721 tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa tatgttaaac
      781 gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata aaagacggtg
      841 taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa gatgtgattg
      901 aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt gagttacctg
      961 acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt tctaaaatac
     1021 cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta tataagacta
     1081 agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat tttttaaata
     1141 acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg aggattcttt
     1201 ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat ttctgaactt
     1261 ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc ctacattttc
     1321 acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg agattcttta
     1381 agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc
     1441 tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa aactattcga
     1501 tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat aaacattgga
     1561 taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg gtgggctgga
     1621 atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt ggcaaatacg
     1681 tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag tagggcaccg
     1741 aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa ttggaatcta
     1801 gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa taaggacaaa
     1861 attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt
     1921 ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg taagcaaagc
     1981 gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat ttttgcagat
     2041 tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc tgtaaaagca
     2101 ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct tgattctaaa
     2161 tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg
     2221 cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa tcccgcagaa
     2281 aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta ttactgtgac
     2341 tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat gctgctgaat
     2401 gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact ttatgtagga
     2461 ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc aggtgctaga
     2521 gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc tggtgatttg
     2581 acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc catgggagtg
     2641 cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc agaagatgtg
     2701 tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat cgatttgatt
     2761 tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa agctttggct
     2821 ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac aatgttcatg
     2881 aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac cagaatgttt
     2941 gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt
     3001 gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga ccactatttt
     3061 gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa caaaggacca
     3121 atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga agaagaaaaa
     3181 ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa aaaaacaggg
     3241 attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat
     3301 gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg tgtttatttg
     3361 acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt tgaaaaatag
     3421 tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa
     3481 tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat tagcactctt
     3541 gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct tcttgctata
     3601 tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag gaagatgtta
     3661 acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag tagtggcgtg
     3721 cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc aacgattcgc
     3781 aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc ttcttctggg
     3841 cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa acctgctcgc
     3901 gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt tcatgaaatc
     3961 aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta tacgactttt
     4021 tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt acccttgaat
     4081 aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc
     4141 actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac
     4201 cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga gattccgatg
     4261 attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga caccgttctt
     4321 ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt ggattatgag
     4381 ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga ctcagacgga
     4441 cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg tgctgaatta
     4501 ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat agatggtcat
     4561 ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg gatgttcggt
     4621 ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta ccgctcgctt
     4681 gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt tctctctttc
     4741 ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat cgtgaaagaa
     4801 aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga aatcgatgtc
     4861 gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga aacagcgaaa
     4921 ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc tgaaatcgca
     4981 aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg ttcacaagat
     5041 ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc cacagaagtg
     5101 tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt
     5161 aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc ctttgatcct
     5221 aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc cgatacgatt
     5281 gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc aactatggtc
     5341 attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag aaggagacgt
     5401 taattatgag taaaattatc ggtattgact taggtacgac taactctgca gtagcagtat
     5461 tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca acaccttctg
     5521 ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt caagctgtga
     5581 caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag
     5641 atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt caatatatca
     5701 aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag
     5761 cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc gctggtctag
     5821 aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt ttagataaaa
     5881 cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt gacgtatcta
     5941 tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac aaccatctag
     6001 gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc aaaaaagaaa
     6061 acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa
     6121 aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca tttatcacag
     6181 caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa tttgatgaat
     6241 taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta aaagatgcag
     6301 gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact cgtatccctg
     6361 cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca gttaaccctg
     6421 atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt gatgtgaaag
     6481 atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg ggtggagtgt
     6541 tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa gtattctcaa
     6601 ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg
     6661 ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca gctcctcgtg
     6721 gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta aatgtatctg
     6781 cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct tcaggtctaa
     6841 cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa gcagataaag
     6901 ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc tctgtggaca
     6961 aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag
     7021 ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg aaaactaaac
     7081 gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa caagctgcac
     7141 aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg
     7201 ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt tcgagagaag
     7261 gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt
     7321 tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca ataggaggaa
     7381 agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa ggagcatcag
     7441 aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct gacatcaata
     7501 aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag attttaagtg
     7561 acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat ccaaattacg
     7621 gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt ggcggcttcg
     7681 gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca gtcgatccaa
     7741 atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt gaagaagcga
     7801 tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat acttgtggtg
     7861 gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat ggtgcaggta
     7921 cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa acttgtgatg
     7981 tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac
     8041 atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa gaaggacaac
     8101 aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt ggtgatttgt
     8161 atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct gaaatttact
     8221 atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa gtaccaactg
     8281 tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg aacttccgtt
     8341 tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag
     8401 taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta cgtgcattcg
     8461 ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc gataagttta
     8521 aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat aaagtagact
     8581 tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
     8641 acaatcatac gttcgcctgg tttt
//
LOCUS       DAZUKA010000120         8663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000120 DAZUKA010000000
VERSION     DAZUKA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.123"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..567
                     /locus_tag="ACG8PN_002263"
     CDS             88..567
                     /locus_tag="ACG8PN_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4862393.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            784..1875
                     /gene="phnW"
                     /locus_tag="ACG8PN_002264"
                     /pseudo
     CDS             784..1875
                     /gene="phnW"
                     /locus_tag="ACG8PN_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            1868..2653
                     /gene="phnX"
                     /locus_tag="ACG8PN_002265"
     CDS             1868..2653
                     /gene="phnX"
                     /locus_tag="ACG8PN_002265"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4862394.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2999..4690)
                     /gene="argS"
                     /locus_tag="ACG8PN_002266"
     CDS             complement(2999..4690)
                     /gene="argS"
                     /locus_tag="ACG8PN_002266"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4862395.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5112..6059)
                     /gene="arcC"
                     /locus_tag="ACG8PN_002267"
     CDS             complement(5112..6059)
                     /gene="arcC"
                     /locus_tag="ACG8PN_002267"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4862396.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6174..7193)
                     /gene="argF"
                     /locus_tag="ACG8PN_002268"
     CDS             complement(6174..7193)
                     /gene="argF"
                     /locus_tag="ACG8PN_002268"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4862397.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7284..8513)
                     /gene="arcA"
                     /locus_tag="ACG8PN_002269"
     CDS             complement(7284..8513)
                     /gene="arcA"
                     /locus_tag="ACG8PN_002269"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4862398.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2494 a         1757 c         1588 g         2824 t
ORIGIN      
        1 ttttaggtat aaaagacgaa tattattggg caatccttct tttttttgtt atgatacatg
       61 tgaaatcttt cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt gctgattaaa
      121 caagtcattg aagaattccc gatccgtacg caagaagaac tattaaacaa acttgggcat
      181 tatggcgtta ccgccacgca ggcaacgatt tctcgagata tccgtgatct aaaaatcgtg
      241 aaggcacctg atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa aggaactcct
      301 gagactagga ctgaggaaga ggatcggctg gttcaaatga ttgaagacat tgtattgaaa
      361 gtcgaacgag tccacttttt gacgatcgtc aataccttgc cagacaatgc tcatttattt
      421 gcatctgttt tagatgagat caaacctccc tctattgtca gtacaattgc tggattcgat
      481 acgatcatgg tcatctcgga aaatgaggag gacgcaaaac gagtagaaga atttcttcac
      541 catccaactg aagtatcact tttttaatca gagcacgtat tcctaatatg gaatgcgtgt
      601 ttttttattt tcaatatttt ccttattaaa taaaccttta cactctttac aaaaatacat
      661 cattttttat tcaatttttt caacaccttg attgaatcac aataatcatc atttatattg
      721 gatgttaaca agagcagctt cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat
      781 catatgtata aattattaac tcctggacca ttgacgactt ctgaaacagt gaaaaatgct
      841 atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca atggataaga
      901 aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt cttaatgcaa
      961 ggtagcggct cctttggggt agaatcggta ttaagttctg tgatcaggcc agaagatact
     1021 gtactgattt gttcaaacgg cgcatatgga caacgaatga tcaacatgtg cgaatgtctg
     1081 aaactgaact ataccgttta tgaggtatcg taacatcaaa tcctccaagc tcatgtgatt
     1141 acacagctga ttgaagagaa tccctctatc tccgcactag caatggtcca tagcgagact
     1201 acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga aaaagatctt
     1261 ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgattcgtgt agccgaacta
     1321 gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc aggcttttct
     1381 tttgtacttg ctaataaagc ggaactagaa aagcgaaaaa atgtgagtcg aagtttgtca
     1441 cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact
     1501 tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga ggcagaagga
     1561 ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtactacg acaaaaaatg
     1621 acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat cacctctttt
     1681 ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt aaagcaaaac
     1741 ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat cggaaatatt
     1801 ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga ctataaggag
     1861 aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg actttggctg
     1921 tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc aactgactga
     1981 caaagaaatc cgcgaaccga tgggaaaatt aaaatgggat catatccaac agcttctttc
     2041 cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc aggaaaaaga
     2101 ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg tcaagcattc
     2161 taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg ggatcaatat
     2221 tggttccacg actggattta ctgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa
     2281 agaagggtat tcaccagact tcatggcaac ttctgaagac gtcgaagggt atggtcgacc
     2341 gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt
     2401 aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg gagtgttgac
     2461 agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt accaaaaatt
     2521 agagatgggg aaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagctgg
     2581 cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacaa
     2641 gaatccagaa tgacttttat acaaaatcag aggtcgtgaa aaagctgtac tgcatcaagt
     2701 aataagaaca gccaaacaaa aatagctcct catatttttc gtttggttag gcttattacc
     2761 gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa agggagctgt gacacgactt
     2821 ttgtcacagt ccccttattt acaaacaaac agtgtttaag agacaacctc tccactaaac
     2881 ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt gaaaagctat
     2941 tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc tctttttctt
     3001 acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat aatacagtca
     3061 ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa atcttcgtat
     3121 gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca atcactgacg
     3181 gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact aatttaacca
     3241 cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg
     3301 ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt ccagtttctc
     3361 cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga tcgtttttca
     3421 aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct ttgttttcca
     3481 aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca tttaatactt
     3541 cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct tgcgtgatca
     3601 aaccaaatgg aatatggcgc atatcgtcag accagtcaaa gcccatttcc ttcaagacag
     3661 ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga gattgtttga
     3721 agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg
     3781 caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg tcaacgatct
     3841 ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg atttcatcca
     3901 tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc agcagatcat
     3961 agattttatt gaattctttc atcgattcat cacggaacca ttgccataat tgaatagcct
     4021 cttcatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt aattctggtt
     4081 ctgtttcagc tacttcatgg aattggacat ataaacgaag caattcattg attggttctg
     4141 ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt ccaaactgtg
     4201 ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt tccataatga
     4261 aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa
     4321 tattaggaga agacatgtcg attggtactg ttccttgatt cccgatattg ctatccccat
     4381 aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt tctttattca
     4441 tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca gaatcgattt
     4501 tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa
     4561 aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt aattgttcga
     4621 cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg acaagatctt
     4681 tattattcat ctttattcct cctaaagtgc tcccttgatt gttaaacaaa aaaaatctct
     4741 agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt accactccaa
     4801 ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg tttaaaacag
     4861 aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact tccgaattcg
     4921 ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta ttaaactttc
     4981 ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt ctgataaaaa aacaatccag
     5041 atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa
     5101 gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg
     5161 atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata
     5221 gctgcctcta ctttcggaag catgcttcct ggagcaaatt gcttttcatc aatgtattgt
     5281 ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca
     5341 tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat
     5401 ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt
     5461 tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc
     5521 gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc
     5581 catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg
     5641 gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca
     5701 ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc
     5761 ttcagtactt cgcccatggc attttgtaac cagtaaccga tagatccttc tgtcattgca
     5821 acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc
     5881 aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg
     5941 ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt
     6001 gcatccgtag acaagattgc attaccccct aaagcgacaa ctacttttcg tttggccatt
     6061 tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt
     6121 gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg
     6181 cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga
     6241 ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc
     6301 atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg
     6361 aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt
     6421 tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca
     6481 cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca
     6541 tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg
     6601 atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt
     6661 gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca
     6721 gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt
     6781 gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt
     6841 tcctctacca ttttttgact gaatccacgg aattcgattc catcaaacat acgacctagt
     6901 actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga
     6961 tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta
     7021 gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca
     7081 cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt
     7141 gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac
     7201 attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag
     7261 taagaccgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg
     7321 cagcgtggac caccacggcc tcgtgacaat tcacttgaat tgatttctaa cactttgatg
     7381 ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct
     7441 ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca
     7501 tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt
     7561 tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag
     7621 acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct
     7681 accattgtaa atacggtatc caaatgcatc attgcacggt tgtttggaat tttgattgct
     7741 aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct
     7801 ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg
     7861 tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt
     7921 gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct
     7981 tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta
     8041 aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatcg
     8101 tatagactgc tagaacgtac ttcaatatct tttgtacgaa ccccagccat gattttatct
     8161 accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc
     8221 gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg
     8281 tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat
     8341 gtctttgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac
     8401 aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccttttcagt
     8461 aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt
     8521 tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat
     8581 tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa ataattaaac
     8641 gtttttctat ttttaagcgt aat
//
LOCUS       DAZUKA010000121         8516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000121 DAZUKA010000000
VERSION     DAZUKA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.124"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..219)
                     /locus_tag="ACG8PN_002270"
     CDS             complement(<1..219)
                     /locus_tag="ACG8PN_002270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862399.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNIS"
     gene            complement(212..841)
                     /locus_tag="ACG8PN_002271"
     CDS             complement(212..841)
                     /locus_tag="ACG8PN_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4862400.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(834..1952)
                     /locus_tag="ACG8PN_002272"
     CDS             complement(834..1952)
                     /locus_tag="ACG8PN_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4862401.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1954..2586)
                     /locus_tag="ACG8PN_002273"
     CDS             complement(1954..2586)
                     /locus_tag="ACG8PN_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4862402.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2597..4414)
                     /locus_tag="ACG8PN_002274"
     CDS             complement(2597..4414)
                     /locus_tag="ACG8PN_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS4862403.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4427..5191)
                     /locus_tag="ACG8PN_002275"
     CDS             complement(4427..5191)
                     /locus_tag="ACG8PN_002275"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4862404.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5219..5917)
                     /locus_tag="ACG8PN_002276"
     CDS             complement(5219..5917)
                     /locus_tag="ACG8PN_002276"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4862405.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5929..6708)
                     /locus_tag="ACG8PN_002277"
     CDS             complement(5929..6708)
                     /locus_tag="ACG8PN_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4862406.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6724..7668)
                     /locus_tag="ACG8PN_002278"
     CDS             complement(6724..7668)
                     /locus_tag="ACG8PN_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4862407.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7714..8493)
                     /locus_tag="ACG8PN_002279"
     CDS             complement(7714..8493)
                     /locus_tag="ACG8PN_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862408.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2272 a         1772 c         1457 g         3015 t
ORIGIN      
        1 ggatatattt tttttatatc cgatcgtttt tatcacatga cgatttttaa tcatatgcct
       61 ttctttactg gatagaaatt gttttcttat atgtgagtaa tcagatacaa cattaaaata
      121 ttcgatactg ttttccgtca gaatttcctc taatatattc gttcgactga tataatcaaa
      181 aggatgaata agcagccact tttttttatt ctcactcatc tatcttcgca ccctcaaatt
      241 tctcagtagt cccacttgct actgatgtta ttccatcacg gacaataact ttctttagcg
      301 ttaaaaaaaa tatttttatg tctttcatca ttgaaatgtt ctccacatat tcaatatctt
      361 tttcaaattt ttctttccaa ctaattgcat tacgaccact gacttgagct aatccggtta
      421 aacctgggcg cacaaaatgt ctttttcgtt gttcattact gtatagtgaa agatactcaa
      481 ctaacagagg acgcggtcca ataatactca tatctccttt taaaatattc cataattctg
      541 gtagttcatc aagactagtc gaacgcaata ttttaccaaa ttgagtcaat ctgatatcgt
      601 ctgataataa attaccttct tcatctcttt gatctgtcat tgttcgaaat ttatacatca
      661 gaaaaatctt ttcatgtttt ccaggtcgtt gttgtttaaa gatcactggc gatccaagct
      721 ttattcgaac taggattgca acaaacgtca tcagtggcca taacattatt aaacctaaaa
      781 ttgaaaaaag aatatctatc aaacgtttaa agaacttttc gtagatacct ctattagaca
      841 ttttgcatca ctcgcttaat tgtattagtt actcgataca aatcatcatc ggtcatttta
      901 gaatctgaag gtaaacagat acctttttca aacaatgtct gagcaacatc tgtaccaata
      961 tactcatact tatcgaaaac tggctgtaaa tgcattggtt tccatatcgg gcgggcttct
     1021 atattatttt tatctaactc atcaataaga tgattcgtat ttattccctt tactgttatg
     1081 acacttaacc aataatttgg ttcattccaa ttattttctg gcatgaattg tatatttgac
     1141 aatttagaca attctttttt ataaaaacta tgaatatatc gttttttctc aattctttca
     1201 tttaaaactt tcaattgtcc tcgaccgata cctgcaacga tattgctcat tcgataatta
     1261 tagccaattt cattgtgttg ataatggcgt gccttctcac gagattgcgt agcccaaaac
     1321 ctcacttttt ccaccttttc tttgtcatta gacacaagca ttccgccacc tgaagtcgtg
     1381 attatcttat ttccattaaa agaataaatt ccatagtcgc cgaatgtacc agtatatttt
     1441 cctttatatg ttgttcctaa tgattctgca gcatcttcaa ttaaaacaac attatgttct
     1501 ttgcatattt tagtaatttc atctagttca gctgataaac cgtacaaatg cactactaat
     1561 acagcttttg cgttaggata tttttctagc gctgttcgca aatgttcagg gttcatattc
     1621 caagtttcgt aatcactatc aacaaatact ggaatagctc cttgatagat gattggattg
     1681 gcagtagctg agaacgtcaa agactgacaa atcactatat catctttacc gaccccagcc
     1741 gcttttaaag ctagatgtat agctgctgtt ccagaagata gtgcagctgc atgactagca
     1801 ccgacatatt ctgctaattc ctcttcaaaa ccattgacat tcatacccaa aggagccacc
     1861 caatttgtat caaatgcttc ttttacatac tcttgttcat atccttcatc actcatatga
     1921 ggagatgcta ataatattcg tttattttcc aacttaatcc catctttcta tgactttggt
     1981 aggtacgcca acatttttag tcttattatt aatatttttt attactacac ttcccgcacc
     2041 tataatgttc atatttccaa ttcttagatt ttgtattatt ttagtaccta ctccaatttc
     2101 atttttatct cctaatagaa catgtcctga aatatttaca cttggaaaaa ttgaattata
     2161 attaccgatt tgagtatcat gtccgatagt agagccgata ttgatcatat tgaaatttcc
     2221 taaaactacg tcagcagtgt aagtcgtata gggcatcaaa atattgccaa ttcctaactg
     2281 aacatttatg cccactagtg cacttgatgc aactaaatta gggaaaatca atttattatt
     2341 tttatttatc ttttgatata ttttctcttt gatatctggc gaagcgattc ccagaaaaat
     2401 agcagtttct tcttctaatt ctaataacga gtctattgtt cccaatacag tttttcccac
     2461 taattcttga ttttgcattt ttaacgaatc atcgacaaaa ccaatcaatt catatctaga
     2521 gaaactttct agtagaaaag caatctcttt gccaaatcca ccagctccta caataattgc
     2581 tttcacttta ctccccctac ttattcgaag cattcgcaaa agtgacaatg tctttagcca
     2641 attgttcatc gtcttgcggc aggctcttga caaattccat cactgtttgc atggaataac
     2701 ctttgatgtt tcccacaaag atcttctcgt aaactgcttc atcgtttctt tccttatcta
     2761 acaatagttc ttcatatagt ttttcccctg gacggatacc tgtttcaatg atttcgatct
     2821 cgtcttcgct atatccgctc aatcggatca tgttttttgc tagatctacg attttcactg
     2881 gttcactcat atctaaaata aagatttcgc cgccttttgc taacgcccca gattgaataa
     2941 ctaaacgact tgcttcagga atcgtcatga aatatcgggt catacgaaag tctgttacag
     3001 tgactggccc tccttgagca atttgttccc ggaatactgg aatcacacta ccgcgagaac
     3061 caaggacatt cccaaatctg acagctgaga atttcgtaca tccttcttca ttcaacccag
     3121 tcacgatcat ttcagcgatt cgttttgttg aacccatgac gtttggcgga ttgttggctt
     3181 tatctgtcga gaccatgacg aagtttttta ctttcgctgc tttggctgct tctgctacat
     3241 tttttgtacc gaaaatattg tttttgactg cttcttttgg gttatattcc atcaatggta
     3301 catgcttatg agcggctgca tggtagacga tatctgggtg gtattgttcc atgatctcat
     3361 tgattttctt gcgatcttgg atatccgcaa tgatcggcac gatttctgta ggacactttt
     3421 gatgatgcgt gcgtaattca cggtcaatca agtaaatcga gttctcccca tgacctaaaa
     3481 gtaatagttt tgcagggtca aattggatga tttgccggca gatttctgaa ccgattgatc
     3541 ctcctgcacc ggtgacaaga atgactttgt ttgtgatctg gtctttgatg gattcgatat
     3601 ccaactctac ctcatctctt cccagtaatt ccactacgtc gatggttttt aattttgata
     3661 cgctgatttt ccctgaggca agttcttcca tagaaggcat cgtattgact ttcacatgag
     3721 cagattcgac taactcgaag attcttcgta atttttttct tgataaagaa gggatagcga
     3781 tcgttaccat ttggatgtca tatttttcga tcaattccgg tatatctttt aatgacccca
     3841 gtactttttt tcctgaaaga taggtatttc ttttattcgg atcatcatcc acgaatccaa
     3901 tgacatggat atcttgagcc gttttagaac caagaaagct gttgtataaa atacgtccac
     3961 cttcgccggc cccaacgatc aaggtatttt tagcggaatc tgttgataga tacctcatat
     4021 tttttgtttc gatatagata cgccagatca atcggctgcc aatgatcagt aatagcgaaa
     4081 gtaggtatgc aaagatcact aaacgtaaac tgtattgttt atttatgaaa agcaatatga
     4141 tgatacttga ggccgcactt gcagataacg aaccaaaaat cgcaatgatc tctcttaaat
     4201 ttgtataacg attgattcga gtaaaaactt tgaataaact accatagaat aaataaaaac
     4261 caatcgacaa accgatagag attaaaataa ggtccattgg tatagctaca aacggcttca
     4321 tgaatttagc tgctgcaata ttcgcagcaa tcaacaacaa gctgtccaca aataccaatg
     4381 tggcaatttt tcttcttctt gttaatacaa acaccctaca ttccccctaa aataaaccaa
     4441 acttcttctt ttttacctct tcatagactg gataactgat ctcatccccg ttgatcaggt
     4501 cctttgcgac ttgtttcatc tgtgctactt tttccttacc aaagtccttg gcgatttgct
     4561 cataacattc ttttaagtaa aaggtacgtt tagagacccc atgggcatcg gaagcagcca
     4621 tttgcacgag gttatgtttg accatcattt tggctgtttt ctggatgtct tttccaaata
     4681 caccgacata acttggtgcg gtgagttgag ccaagcatcc catatctagg aatggcagca
     4741 aatgatttgg gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtgtct
     4801 ttcctagttg taacaactca aaaaagagcc attctgcata taacggcact tccagtgtcg
     4861 gaaattcgat tagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga
     4921 tgtcttcaat cagtgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca
     4981 attgtctttt ctctaattct gcttgtaacg aagcgactag agaaatgact tgtgattttt
     5041 cattgctgta ttttccatta ttgtgatggg gtgtgcataa aatatgtgtg atcccttgtt
     5101 gaatcgcttt ttcagccatg gcgatactgg catctatgtt ttctgctccg tcatcgattc
     5161 ctggcaggat atggcaatgt agatcgatca tttctcttgt ctcctctctt tttatttctt
     5221 aattcccgta gtaatagcca gcatctctgc tgtgttctgc tccattgtat acaacaccaa
     5281 ggactcgcgc ttgtaccata tctaacagtt cttttgcttt agtcaatgat tcttttcgtg
     5341 acgtattttc ccgtacgacc aataaggtgc catccgtttt tgaagacatg atctgtgcat
     5401 ctgttacggc aacgactggt ggcatgtcga aaatcactac atcaaatagt tgtcttgctt
     5461 ctgctaaaat ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag
     5521 gtccacttgg cagaatcgac aaattgtcga ctggtgttcg ttggatctca tctgctacac
     5581 ttcctgatga actaagaaca gtacttaatc cgcttgcgtt gttcaacttg aacgttttat
     5641 agatgacggg cttacgtaag tctgcatcga ctagcagtac ccgttgacca gatttggcaa
     5701 aaacaacggc aatgtttgct gcagtggtgg attttccttc tccaggacct gatgaagtca
     5761 cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg
     5821 tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat
     5881 gagtttgagt tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga
     5941 gcggcttctg cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctga
     6001 tcctttttta cggataccag cgttcaattc ttttgctgac atttctggaa ctgttcctaa
     6061 gattgggaat cctaatgtat ccgtcacata tttttcatct ttcactgtcc gatcaagtaa
     6121 ttcgaataag aatgccagac ctacgcctac catcattcct aacactaagc caatcactag
     6181 attcaattta ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga
     6241 gattttatcg acattcatca cgtctttggc gttttcttgg aaaacttgtg aagttgtatt
     6301 agcgatctgt tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga
     6361 attttgtgac tgattgacgg agatcgcatc tttgatttgt ccagcagtca ttttcacatt
     6421 gtcttctgtt tctaaacgat ctttgacttc atttagtact aaatcactga cgatcatatc
     6481 tttatacgta ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga
     6541 ttgtggcagt gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa
     6601 tgttacgatt cctgagatgg ccaagccagc aaacattgag atgatgattg ttaccaagtg
     6661 tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact
     6721 ttcctattta ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc
     6781 ttgatcgctg actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg
     6841 atcttgcttg atcgtaccaa atgcctcccg ataatctaaa gcgatttttt tcatttcatc
     6901 aaagctcatg tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct
     6961 gacaccgttt aaactcaata ctttttttgc tacaccttct acgatctttc tttgacgctc
     7021 ttgtctacca tagtcaccat ttggatcttc ataacgcata cgagaataag ataatgcctg
     7081 atccccgttc aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag
     7141 gtcattattg acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt
     7201 gatagagaca tagtgatcga ttgggatgtc tacatatttt tgtagggtat ccatcgccat
     7261 ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat
     7321 gtcgacatac gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac
     7381 tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc
     7441 aatgcccatc aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg
     7501 ttctccttgg gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag
     7561 atataatttt gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat
     7621 aatcagaaat atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt
     7681 ttgctaacct tgcttgaatc aacgttttaa ctgtcaataa cttatattgc tatagcccat
     7741 ttgctgagca acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat
     7801 ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg
     7861 accatccttc aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt
     7921 atcagcgaat cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct
     7981 catattttca cgtacacgag cgacatctgc acttgtcacc ttttctttga tgacgacatc
     8041 gtctacagct tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg
     8101 gatcttcagt tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg
     8161 gattgcaaca gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag
     8221 tttgacagag gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt
     8281 tagaaaattt ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa
     8341 ggtgtctttt tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg
     8401 ctgattattg atatggtaac caaacaaaca gatcgcaata gcacctgcac ctaccgccac
     8461 agcagtcaat gacttgacga atggcttttc catagattca ctcctaagta ttgatg
//
LOCUS       DAZUKA010000122         8511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000122 DAZUKA010000000
VERSION     DAZUKA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.125"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      16..95
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            93..1286
                     /gene="metK"
                     /locus_tag="ACG8PN_002280"
     CDS             93..1286
                     /gene="metK"
                     /locus_tag="ACG8PN_002280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4862409.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1416..2888
                     /locus_tag="ACG8PN_002281"
     CDS             1416..2888
                     /locus_tag="ACG8PN_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4862410.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            2971..3759
                     /locus_tag="ACG8PN_002282"
     CDS             2971..3759
                     /locus_tag="ACG8PN_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4862411.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3826..4494)
                     /locus_tag="ACG8PN_002283"
     CDS             complement(3826..4494)
                     /locus_tag="ACG8PN_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4862412.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4609..6321
                     /locus_tag="ACG8PN_002284"
     CDS             4609..6321
                     /locus_tag="ACG8PN_002284"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862413.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6324..8096
                     /locus_tag="ACG8PN_002285"
     CDS             6324..8096
                     /locus_tag="ACG8PN_002285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862414.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8178..>8511)
                     /locus_tag="ACG8PN_002286"
     CDS             complement(8178..>8511)
                     /locus_tag="ACG8PN_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4862415.1"
                     /translation="IIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKE
                     LNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLV
                     EKLGKIEI"
BASE COUNT         2591 a         1415 c         1856 g         2649 t
ORIGIN      
        1 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
       61 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      121 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      181 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      241 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      301 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      361 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      421 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      481 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      541 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      601 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      661 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      721 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      781 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      841 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
      901 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
      961 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1021 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1081 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1141 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1201 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1261 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1321 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1381 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1441 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1501 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1561 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1621 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1681 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1741 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1801 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1861 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     1921 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     1981 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2041 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2101 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2161 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2221 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2281 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2341 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2401 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2461 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2521 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2581 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2641 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2701 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2761 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2821 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     2881 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     2941 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3001 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3061 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3121 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3181 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3241 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3301 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3361 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3421 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3481 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3541 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3601 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3661 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3721 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3781 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3841 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     3901 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     3961 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4021 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4081 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4141 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4201 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4261 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4321 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4381 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4441 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4501 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4561 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4621 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4681 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4741 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4801 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4861 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     4921 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     4981 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5041 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5101 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5161 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5221 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5281 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5341 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5401 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5461 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5521 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     5581 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     5641 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     5701 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     5761 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     5821 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     5881 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     5941 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6001 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6061 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6121 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6181 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6241 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6301 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6361 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6421 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6481 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     6541 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     6601 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     6661 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     6721 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     6781 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     6841 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     6901 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     6961 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7021 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7081 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7141 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7201 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7261 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7321 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7381 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7441 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7501 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     7561 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     7621 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     7681 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     7741 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     7801 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     7861 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     7921 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     7981 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     8041 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     8101 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     8161 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
     8221 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
     8281 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
     8341 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
     8401 gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa aatgtattta
     8461 tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat t
//
LOCUS       DAZUKA010000123         8447 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000123 DAZUKA010000000
VERSION     DAZUKA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.126"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1107)
                     /locus_tag="ACG8PN_002287"
     CDS             complement(139..1107)
                     /locus_tag="ACG8PN_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4862416.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1339..2172
                     /locus_tag="ACG8PN_002288"
     CDS             1339..2172
                     /locus_tag="ACG8PN_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4862417.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2206..3045
                     /locus_tag="ACG8PN_002289"
     CDS             2206..3045
                     /locus_tag="ACG8PN_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4862418.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3071..3631
                     /locus_tag="ACG8PN_002290"
     CDS             3071..3631
                     /locus_tag="ACG8PN_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4862419.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3830..5551
                     /locus_tag="ACG8PN_002291"
     CDS             3830..5551
                     /locus_tag="ACG8PN_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4862420.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5716..6858
                     /locus_tag="ACG8PN_002292"
     CDS             5716..6858
                     /locus_tag="ACG8PN_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4862421.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6874..8085
                     /gene="thiI"
                     /locus_tag="ACG8PN_002293"
     CDS             6874..8085
                     /gene="thiI"
                     /locus_tag="ACG8PN_002293"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4862422.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2902 a         1458 c         1753 g         2334 t
ORIGIN      
        1 gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc aaatttttgt
       61 aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt tttaagttta
      121 gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac ccgacaaacc
      181 atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac tgttacagaa
      241 aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa caggctctgc
      301 gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa gaagcttttc
      361 caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga ttaccaaacc
      421 gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag catatgcttt
      481 aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt ctaaaagttc
      541 ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg taggtgttcg
      601 gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat ggatctcatc
      661 gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga cacgaatcgc
      721 actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca ttgccccagt
      781 caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag tttcttctaa
      841 tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag cggacaagat
      901 acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa gattgaagat
      961 atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta ataatgggtt
     1021 tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga tcgttccgcc
     1081 ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa ttttgcatag
     1141 ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag aataacgaat
     1201 agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca accttgacct
     1261 cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca aggaaatcaa
     1321 tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga acatgacaaa
     1381 ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc cttagtggaa
     1441 ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg gataagtgaa
     1501 gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg tcaaggagac
     1561 tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga tgttatgtcg
     1621 ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc atcattcaaa
     1681 tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga gggttatcct
     1741 gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga acgtacaaga
     1801 aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca gttgctgaat
     1861 acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt tcgattccat
     1921 ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa aagaacgtta
     1981 tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt agttgattct
     2041 aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc ggctgaaaat
     2101 gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc aacccaaatg
     2161 gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat caaagctatt
     2221 ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga atcaacaaaa
     2281 caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac ggggcgcagc
     2341 ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt cgtgtataat
     2401 ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga acaaaaagta
     2461 ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt tggtgcgaga
     2521 aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat caagcgacta
     2581 gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct ccaggttttt
     2641 agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga accaatttat
     2701 caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg ctttcccgag
     2761 tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg tggttcaaaa
     2821 ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga aattatggca
     2881 ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg agtggccatg
     2941 gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc aaatgaagaa
     3001 gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat gaaagaagga
     3061 aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc acaaaacttt
     3121 tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc atacacgtgc
     3181 tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta ataaagaaca
     3241 actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag aaaaaatact
     3301 tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac tgacagatat
     3361 cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc gaatattcga
     3421 aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc attatgaccc
     3481 tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc cagaacctaa
     3541 aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga aatccgctga
     3601 tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt gttatcttta
     3661 tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa tagtatgcta
     3721 taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat gccaagcaga
     3781 acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa tgggaaataa
     3841 tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct ttttggttag
     3901 ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac gcaaggaaga
     3961 attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc atctggttgg
     4021 gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat ctacttcctc
     4081 gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa gttttcggtt
     4141 cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa tggagaaaca
     4201 agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac gcaactcact
     4261 agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca gagaagacga
     4321 gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga tcgaaaaaga
     4381 atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc gcgaggtttt
     4441 ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa ttccaccgct
     4501 gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg atacttacga
     4561 gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag atatcgcaaa
     4621 agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag ttgaaacggt
     4681 cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata tcttagaacg
     4741 ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag agtatatcaa
     4801 acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat ctcaaagcta
     4861 tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag tggaagaaat
     4921 ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc cttattcaga
     4981 agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa agcaacaagt
     5041 agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc aagaaaagat
     5101 cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac aacgtttgcc
     5161 tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta ttgaagaact
     5221 aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt tagttgctct
     5281 atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag atgcagcagc
     5341 attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg aagaagtgag
     5401 agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc aagaagcatt
     5461 agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac gcatcgaaga
     5521 tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac cagcaagagc
     5581 aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg aaacaagtga
     5641 aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa gaatttcaaa
     5701 aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat ctttccgcaa
     5761 agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc aagcttgcat
     5821 gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat cgctgatttg
     5881 atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg agataactgg
     5941 gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat catttcagga
     6001 gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg aatcgaagta
     6061 agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa agaattaata
     6121 agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg atcgattcag
     6181 cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt ccatgtagat
     6241 gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag agttgatttt
     6301 gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat ttattggaaa
     6361 aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa tcagcgaagt
     6421 gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt atcattagag
     6481 ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat caatgcgttg
     6541 aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc gcatatttta
     6601 tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga agaaaaacaa
     6661 atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag cagtacattg
     6721 cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag cttagatgaa
     6781 aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct ttataagaaa
     6841 ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat tatggtccgt
     6901 tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca actagcacaa
     6961 aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga tcgggaccgg
     7021 atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact agcaaaaata
     7081 tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga agttttgaaa
     7141 caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt taaaattatc
     7201 gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca aacattaggt
     7261 aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc agatattcca
     7321 ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat caagggagct
     7381 ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg ggggattgat
     7441 tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc tgttcatttt
     7501 gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct aacagccaaa
     7561 ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga gattcaagaa
     7621 gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg aatgatgttg
     7681 cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa tggtgaatca
     7741 ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga agtaacgaat
     7801 acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga agtagcagaa
     7861 cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac gatttttgct
     7921 cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga agagcgttta
     7981 gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga gatacaagca
     8041 gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt tatgaaaata
     8101 gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt attttaccgt
     8161 ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag ttatggaaaa
     8221 gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag ctacagttga
     8281 taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag aagtgggcag
     8341 cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg aaatttcggc
     8401 ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAZUKA010000124         8324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000124 DAZUKA010000000
VERSION     DAZUKA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.127"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            280..1527
                     /locus_tag="ACG8PN_002294"
     CDS             280..1527
                     /locus_tag="ACG8PN_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4862423.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            1795..4185
                     /locus_tag="ACG8PN_002295"
     CDS             1795..4185
                     /locus_tag="ACG8PN_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4862424.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            4234..6069
                     /locus_tag="ACG8PN_002296"
     CDS             4234..6069
                     /locus_tag="ACG8PN_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4862425.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            6144..7001
                     /locus_tag="ACG8PN_002297"
     CDS             6144..7001
                     /locus_tag="ACG8PN_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4862426.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            6991..8100
                     /locus_tag="ACG8PN_002298"
     CDS             6991..8100
                     /locus_tag="ACG8PN_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4862427.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         3116 a         1298 c         1669 g         2241 t
ORIGIN      
        1 caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa tcgcaaacaa
       61 aaagaaattg aacctagaca aatacaactt gtataccgca agggaagcaa gtttgatttt
      121 gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc gtttgcctaa
      181 aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag gaatagaagc
      241 attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa gagaaattcg
      301 cttggtatgg ttttattggc atcagctgta cttgcggggt tatcagtacc agtaacctca
      361 catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac aggacaatca
      421 agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct taaaagctta
      481 ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt ggcaaaagga
      541 gaacaacgga ctatcccgat tggacgaact ttcaacatca gtaaagatac attaacagtt
      601 aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc tgatcctatt
      661 gaaaatacgg agcaagagaa caaaaaatcc gagactacaa aagaatcgga accaacagca
      721 aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag ttcatctaat
      781 acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag tagcactacg
      841 gaagctgata ctggaacatc aacaaataac tcacaatcaa catcaaatga tgcaaatgaa
      901 tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga aactgacagt
      961 tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt tccatttatt
     1021 agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt acattataaa
     1081 ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact atctcctgac
     1141 aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg aacttatcaa
     1201 ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa tgttgtagat
     1261 atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa tcctgtagta
     1321 gcgtgggtaa cttatgcctt tgaacctgct caacataaac caatgttttg gggcgatgct
     1381 atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta ccatgttgtc
     1441 gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc atataacgta
     1501 tctcaattag cggtttcggt acgttaataa aagatatcgt ttatatacta agtagttata
     1561 tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac
     1621 taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaaa
     1681 tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga
     1741 catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag
     1801 catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg
     1861 atgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa
     1921 ccgacaagtg caaaatttat agaaactggc gtaaaaacag acggttatat tagggttaat
     1981 atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat
     2041 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
     2101 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
     2161 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
     2221 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
     2281 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
     2341 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
     2401 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
     2461 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
     2521 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
     2581 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
     2641 tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
     2701 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
     2761 gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat
     2821 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
     2881 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
     2941 gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt
     3001 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
     3061 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
     3121 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
     3181 actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
     3241 tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat
     3301 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
     3361 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
     3421 tatagcctag aagaaaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
     3481 atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
     3541 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
     3601 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
     3661 acgttcaaca ttattgcgaa caaaacttac aacttagaag aaacaaaagt accaacaggt
     3721 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
     3781 tatacagcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt cgatgatcaa
     3841 gggaaagcat tagcaaacgc agaattcact ttgacggacg acaaaggtgt aaaagttgta
     3901 caaaaaacgg acagaaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
     3961 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
     4021 gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
     4081 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
     4141 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
     4201 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
     4261 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
     4321 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
     4381 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
     4441 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
     4501 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
     4561 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
     4621 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
     4681 aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt
     4741 gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat
     4801 caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca
     4861 gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga
     4921 aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta
     4981 actgtggatg gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat
     5041 gctaatagaa caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg
     5101 gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta
     5161 attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt
     5221 cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta
     5281 acttatgatt catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa
     5341 cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa
     5401 caatttgatg caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa
     5461 gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa
     5521 cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt
     5581 aaaacagacg gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa
     5641 attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaagc tgttacttgg
     5701 attgcagata aatctaaagc tactgaattc gtttccggat cagacggtaa gttccaaatt
     5761 gaaggtttgc aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca
     5821 gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca
     5881 actagaaacg atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac
     5941 atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt
     6001 ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac
     6061 aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa
     6121 gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa
     6181 ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct
     6241 actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa
     6301 aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag
     6361 aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc
     6421 agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt
     6481 tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg
     6541 gaactgatta tccaactggt gggattggaa accattctgt tatatcagct cattcaggca
     6601 ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta
     6661 ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa
     6721 ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta
     6781 cacctgttgg agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa
     6841 aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact
     6901 ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa
     6961 aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact
     7021 cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa
     7081 acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa
     7141 gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac
     7201 aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt
     7261 gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat
     7321 caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca
     7381 aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt
     7441 gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata
     7501 gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc
     7561 ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca
     7621 gacgttatta gaaattacgg tcagaattca acactgactg atattcctga tttagatatg
     7681 ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt
     7741 ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac
     7801 gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac atcttctaat
     7861 gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat
     7921 attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca
     7981 atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtaata
     8041 ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg aaaagcataa
     8101 tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct
     8161 tttttatttt gaatagagct agattcactt ttaaataaaa tcaaaactga tcttaattct
     8221 aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt atcagaatat
     8281 tattagaaaa gataaactca aaagcttaat ttaatagggt tatt
//
LOCUS       DAZUKA010000125         8286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000125 DAZUKA010000000
VERSION     DAZUKA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.128"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..2631
                     /gene="clpB"
                     /locus_tag="ACG8PN_002299"
     CDS             22..2631
                     /gene="clpB"
                     /locus_tag="ACG8PN_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4862428.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2782..3468)
                     /locus_tag="ACG8PN_002300"
     CDS             complement(2782..3468)
                     /locus_tag="ACG8PN_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862429.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3446..3940)
                     /locus_tag="ACG8PN_002301"
     CDS             complement(3446..3940)
                     /locus_tag="ACG8PN_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4862430.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3960..5180)
                     /locus_tag="ACG8PN_002302"
     CDS             complement(3960..5180)
                     /locus_tag="ACG8PN_002302"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862431.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5327..7162
                     /gene="lepA"
                     /locus_tag="ACG8PN_002303"
     CDS             5327..7162
                     /gene="lepA"
                     /locus_tag="ACG8PN_002303"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4862432.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7358..8269
                     /locus_tag="ACG8PN_002304"
     CDS             7358..8269
                     /locus_tag="ACG8PN_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862433.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         2804 a         1495 c         1680 g         2307 t
ORIGIN      
        1 taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg
       61 attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat
      121 gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac ggatgccgga
      181 ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt
      241 tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat
      301 gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt
      361 ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta
      421 tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca
      481 caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa
      541 gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc
      601 cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg
      661 aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat
      721 ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc
      781 cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga
      841 cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga
      901 agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc
      961 ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt
     1021 cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt
     1081 ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta
     1141 gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc
     1201 gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa
     1261 ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa
     1321 gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt
     1381 cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct
     1441 gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat
     1501 aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa
     1561 gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc
     1621 gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa
     1681 cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg
     1741 attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga
     1801 ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga
     1861 aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta
     1921 cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct
     1981 ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct
     2041 tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg
     2101 ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa
     2161 aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact
     2221 gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac
     2281 ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta
     2341 gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat
     2401 caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat
     2461 gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca
     2521 ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta
     2581 cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt
     2641 gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat
     2701 aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg
     2761 cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa
     2821 gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat
     2881 cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt
     2941 gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc
     3001 ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa
     3061 ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc
     3121 aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat
     3181 actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa
     3241 caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa
     3301 aatcaatgca atccctaata aagattccac gataaatgtg attccttgag aagtcgaata
     3361 tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa
     3421 agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt
     3481 ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac
     3541 ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga
     3601 tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga
     3661 tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat
     3721 ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca
     3781 ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga
     3841 tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat
     3901 cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat
     3961 caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt
     4021 gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc
     4081 atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt
     4141 aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga
     4201 gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt
     4261 tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg
     4321 atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc
     4381 cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga
     4441 tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca
     4501 cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg
     4561 aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg
     4621 gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata
     4681 ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca
     4741 gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata caaataagag
     4801 atccattgct tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat
     4861 ccatggttag catattctgg ataagttcca actccagtca caccacccat tttataacgt
     4921 gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg
     4981 tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct
     5041 cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa
     5101 agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct
     5161 ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa
     5221 aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt
     5281 cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa
     5341 aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg
     5401 gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat
     5461 gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt
     5521 aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat
     5581 tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga
     5641 gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt
     5701 ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc
     5761 cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag
     5821 tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat
     5881 cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt
     5941 tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat
     6001 ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga
     6061 ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg
     6121 ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt
     6181 agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta
     6241 ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa
     6301 actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt
     6361 tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga
     6421 acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa
     6481 aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat
     6541 tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg
     6601 tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga
     6661 tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt
     6721 cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta
     6781 tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt
     6841 aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact
     6901 gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa
     6961 aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg
     7021 tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat
     7081 gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga
     7141 tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa taaacctttt
     7201 tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc atttggttat
     7261 cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt tagctgtcag
     7321 tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt caagtggaag
     7381 ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag ctgatttagc
     7441 aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa tccctaaaga
     7501 cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag atgaggaaga
     7561 taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata agttacttaa
     7621 caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg tgttaaatag
     7681 aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat taagtaatga
     7741 aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat caaaagacga
     7801 taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag agacgcttga
     7861 aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta gatgggaaaa
     7921 aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac
     7981 tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag atgaaagagt
     8041 tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga aaattaggaa
     8101 agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa tttcaaaatg
     8161 gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa ttatccaata
     8221 tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa aatagtttac
     8281 gtcgat
//
LOCUS       DAZUKA010000126         8108 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000126 DAZUKA010000000
VERSION     DAZUKA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.129"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1005)
                     /locus_tag="ACG8PN_002305"
     CDS             complement(121..1005)
                     /locus_tag="ACG8PN_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4862434.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(1092..1991)
                     /locus_tag="ACG8PN_002306"
     CDS             complement(1092..1991)
                     /locus_tag="ACG8PN_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4862435.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(2008..2514)
                     /locus_tag="ACG8PN_002307"
     CDS             complement(2008..2514)
                     /locus_tag="ACG8PN_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4862436.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(2703..3323)
                     /gene="rpoE"
                     /locus_tag="ACG8PN_002308"
     CDS             complement(2703..3323)
                     /gene="rpoE"
                     /locus_tag="ACG8PN_002308"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4862437.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(3399..3836)
                     /locus_tag="ACG8PN_002309"
     CDS             complement(3399..3836)
                     /locus_tag="ACG8PN_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4862438.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            3999..4829
                     /locus_tag="ACG8PN_002310"
     CDS             3999..4829
                     /locus_tag="ACG8PN_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4862439.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            4813..6180
                     /locus_tag="ACG8PN_002311"
     CDS             4813..6180
                     /locus_tag="ACG8PN_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4862440.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            6199..7008
                     /gene="yidA"
                     /locus_tag="ACG8PN_002312"
     CDS             6199..7008
                     /gene="yidA"
                     /locus_tag="ACG8PN_002312"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4862441.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(7051..7494)
                     /locus_tag="ACG8PN_002313"
     CDS             complement(7051..7494)
                     /locus_tag="ACG8PN_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4862442.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            7628..7999
                     /locus_tag="ACG8PN_002314"
     CDS             7628..7999
                     /locus_tag="ACG8PN_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4862443.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
BASE COUNT         2339 a         1655 c         1477 g         2637 t
ORIGIN      
        1 aaaattggtg gacagttttt tgttgtacct gtatagatat tgttaagcct ttgcaagtaa
       61 ggtagttatg tgttcaacta cttattaaaa tgtgttattt attaaagata cttgtctcac
      121 ttactgaact ggccgatgat cgtttctgcc ataatagttc tcgacagccg ttggatgccc
      181 taataaatcc ccaactgtcg tgatgcgata cccttgctct ttcagataat cgatcacttg
      241 aggaacggca cgaatcgttt caggatagat atcatggaat aaaataattg ccccatcgta
      301 tactgttgct tggatcttct gcaaaatcaa atcaggattt cttgtcttcc aatcttctga
      361 atcaacagac cattcgatga tcggtcgctg gatgatcgtt gctcctacat tcgtgacaga
      421 accatagggc ggacgaacaa aagtgggggt tttgccagtg acttttttga tctcagtgtc
      481 tgctttcgtc acttcctccg tcatcgactg ggcagaaaga gtggccagat tctgatgatc
      541 gtatgtgtga ttgccaatct cgtgccctgc ttcagctgtc tgcttcacga tttcaggatg
      601 ctgcgccgta ttttctccta agacgaaaaa agtggcatgc acgttttttt cttttaagat
      661 tcgcagaagc tgcggtgttg tggtcgctcc tggtccatca tcaaagctga ttgcgacgct
      721 tttgacagcc ggatctcggc tcgtttttgt actgtcttgt gttgattgtg atgactgact
      781 ttgtaccttt tccgctgatt cttgaatggc ttctttgacg ttagctgttt cagttgattg
      841 gcttttaatg gttgattgtt ctgattgttt gaaagattgt gacgaatgct ccgaagagtg
      901 tgtttgtcct tttacttcgg tgatcaagga ggccaccgaa gtaatttcga aaaacaaaag
      961 cacggataaa gcaataatac tgataactaa ataatttctt ttcactatat ctcttctttc
     1021 tctttttctc ttcttattat ttacttgtac ttacttgaaa attgaaactg ctagtcgcaa
     1081 ctacttctcg tctaatgatc caaataatct tccgatgcca aaatcgtttt tgtcacggac
     1141 ttaggaatga caaattctgg tgtacccata taaccaggag caatttcgta ttgagaaaaa
     1201 gtcaaaacta attgatgctc tttgttcaaa taaaaacttc gtgtaggcgt aacaagtgtt
     1261 ggtttttcta tcgtcccttc ttgaatatct tgctcagtcc aatagtactt agatggatct
     1321 tccttgactt gttcggccat ctgccgtttg acttcttttg tcaaaacaga tagataggtg
     1381 tcatctttga ataaaagtgg aagtgagagc tggacactgt ttttcttgtc gattgtgtcg
     1441 tatctttttt caacatggct atctgctgct gtttgggtga tctgcctttc aatcacgaga
     1501 aaccggttat cgtctacgat tttctgataa tctcccaaaa cttggtaatg atccgtttca
     1561 aaatttccat attctttttc agctttttca aaatctgctt gtccttcgcg aaagtatttt
     1621 tgatttaatc catgaattgt tttattttct ttctcatttt gtgtattgat cttcggtacc
     1681 tgaatatcga ttttttcaga tcgatcagta aagcgttcgc ctaagatcaa ttcaatgact
     1741 gatcctaaga tgggtaaatc ttctgcaaag cttcgaactt gactattaaa aagaaggcta
     1801 cttgttgtaa tcattacgat agccaaagat actccgaacc cttggagacg tctctttctc
     1861 cgctttgctt ttataaatga ttgctcttcg ataataaaac gctggcggat ctcctcttta
     1921 agccctttgg gaattgcttg ttctttataa gttttcttta gtttttttaa attttctttt
     1981 tgggaataca tggcactctt ctctccttca ttctaggatt tgacgtaaag cattcaatga
     2041 gcggtacatt ttcgttttaa ctgtgttttc attcatatcc aagatagtcg caatttcttt
     2101 caaacgaaat ccttcaaaaa agtagaggat cagaatctct ttttccggac tgtctatttt
     2161 atctaaaatc atatggagct ctgcaacgga agtttctaaa ggtaactgtt gggggatatc
     2221 ttgaatttca aataagtcca caggcgtcct tttttgtctg cgccaactat ctaatgccgt
     2281 gcggatcaat atccgataaa accatgcact aaaatgctcg atctcttttt ctttttcaaa
     2341 atagttcagg gctttatgaa aactatcttg catcacatct aaggcctctt ctttatcatg
     2401 tacgtaattc atcgtgatac ggaacatttt ttgataatca gtttcgatga gtccattcag
     2461 caggtgacgg tatttttcga tctgccgttt tttcttatat ccagtaaaaa tcattctcca
     2521 ctcctttctg cctcacttaa tatgacgtac aaggtgcgct aaaagtttca atttttttat
     2581 tttttatgta tttacttggg atttttatat acaaaaaaga aggtgtgaca aaaatcgtgt
     2641 cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta agaaaataat
     2701 gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc atcatcgtct
     2761 tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc atcatctgtc
     2821 gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc ttcatcttca
     2881 tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt atcttctggg
     2941 tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt gactttcttg
     3001 cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt tacttcttca
     3061 tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc taatgagata
     3121 aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg aatttcactg
     3181 tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc catgacatcg
     3241 ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct tttgtttgca
     3301 ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact ccttatcata
     3361 caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa atccaaggtc
     3421 aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat tcgaaccttc
     3481 cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt agcaatctgg
     3541 atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc aaatttcaaa
     3601 cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac taatttgatc
     3661 gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat gtacagcgta
     3721 tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac ttccccttgc
     3781 tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa ttccattatt
     3841 cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt gtcaaccaac
     3901 ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta gagtataata
     3961 ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg attcgactag
     4021 atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat atattaacgg
     4081 aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac tgcacagtca
     4141 tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa acactggaag
     4201 atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta tcagatgaag
     4261 gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata accatagatg
     4321 acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca ccgatacaag
     4381 cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc catggcaaaa
     4441 aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg atgtacttaa
     4501 gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat caatctgctt
     4561 taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca atggcaacac
     4621 ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca tttgcaaaag
     4681 cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg ctaaaaaaag
     4741 aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt aataacataa
     4801 aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga aaaagttttt
     4861 cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga tttgatcaat
     4921 tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc tttcactttt
     4981 cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat ttcaagacga
     5041 atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg aggctgggat
     5101 gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg tcatggtcct
     5161 tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac tgtagagatc
     5221 atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga aggttttcct
     5281 gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt ccaaatgatt
     5341 tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta tttcactgga
     5401 acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc ttatgaaggc
     5461 gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt tagccgatat
     5521 caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt aattttggag
     5581 catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt tgaactccat
     5641 tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata cctcaaactt
     5701 gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga tcctatttta
     5761 aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac tttttctggc
     5821 aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt cggatataat
     5881 cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt ttatcgccca
     5941 gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac tttgatagaa
     6001 ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg agatgagcga
     6061 tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga tttatttgac
     6121 gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga aataaattaa
     6181 aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga tggtacttta
     6241 ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc tgctgttgca
     6301 aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca ggaacagtta
     6361 aacgagttga acctttttca agataatgat tacgttatta cttataacgg ggcactcgta
     6421 caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga agatttccta
     6481 gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga tgacaaagcg
     6541 atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag tttagtcaag
     6601 atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat cgtcaaaatg
     6661 atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc gcaaactttc
     6721 cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact aaataaagaa
     6781 gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga tcaagatgaa
     6841 atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc tggtctagga
     6901 gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat cacttcatca
     6961 aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata aataaaaaaa
     7021 ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga tccatctctc
     7081 gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag ggctcaccca
     7141 ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat cagcaacaaa
     7201 gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat actcatcagc
     7261 ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca gctcacggac
     7321 aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa aaaaagcacc
     7381 atttggagcc tcgattactg caatctgctg atctctttct atgacaatat atgctgcata
     7441 acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat ccatgaatag
     7501 tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct catgaacaag
     7561 cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga aaaggagaga
     7621 tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag catcaatcga
     7681 attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc cagaaaacaa
     7741 atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt tgacttacaa
     7801 ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta tagcagctga
     7861 tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta ctgacttaaa
     7921 aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg gttataaaat
     7981 cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat aaaaaacaaa
     8041 caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt gcgtcttttt
     8101 ttaacaac
//
LOCUS       DAZUKA010000127         8025 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000127 DAZUKA010000000
VERSION     DAZUKA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.130"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACG8PN_002315"
     CDS             382..570
                     /locus_tag="ACG8PN_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4862444.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACG8PN_002316"
     CDS             648..1661
                     /locus_tag="ACG8PN_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4862445.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACG8PN_002317"
     CDS             complement(1728..2762)
                     /locus_tag="ACG8PN_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4862446.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACG8PN_002318"
     CDS             2877..5102
                     /locus_tag="ACG8PN_002318"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4862447.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACG8PN_002319"
     CDS             5120..7003
                     /locus_tag="ACG8PN_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4862448.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACG8PN_002320"
     CDS             6984..7958
                     /locus_tag="ACG8PN_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4862449.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2587 a         1465 c         1700 g         2273 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatg
//
LOCUS       DAZUKA010000128         7885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000128 DAZUKA010000000
VERSION     DAZUKA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.131"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..1920
                     /locus_tag="ACG8PN_002321"
     CDS             154..1920
                     /locus_tag="ACG8PN_002321"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4862450.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2022..3281
                     /locus_tag="ACG8PN_002322"
     CDS             2022..3281
                     /locus_tag="ACG8PN_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4862451.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3428..4729
                     /locus_tag="ACG8PN_002323"
     CDS             3428..4729
                     /locus_tag="ACG8PN_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862452.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4732..5583
                     /locus_tag="ACG8PN_002324"
     CDS             4732..5583
                     /locus_tag="ACG8PN_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4862453.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5596..6411
                     /locus_tag="ACG8PN_002325"
     CDS             5596..6411
                     /locus_tag="ACG8PN_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4862454.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6562..7728
                     /locus_tag="ACG8PN_002326"
     CDS             6562..7728
                     /locus_tag="ACG8PN_002326"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4862455.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2560 a         1362 c         1574 g         2389 t
ORIGIN      
        1 ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa
       61 aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg
      121 atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga
      181 ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact
      241 aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact
      301 gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat
      361 tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt
      421 ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat
      481 gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc
      541 actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca
      601 aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac
      661 cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg
      721 gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa
      781 tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa
      841 aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat
      901 catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc
      961 tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta
     1021 ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa
     1081 ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata
     1141 aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt
     1201 tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag
     1261 atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat
     1321 gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg
     1381 gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc
     1441 aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa
     1501 ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac
     1561 gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg
     1621 gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg
     1681 aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt
     1741 gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat
     1801 cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt
     1861 cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga
     1921 aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat
     1981 aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga
     2041 aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca
     2101 atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg
     2161 attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg
     2221 tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg
     2281 ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag
     2341 gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag
     2401 acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc
     2461 ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg
     2521 agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat
     2581 ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct
     2641 atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg
     2701 tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag
     2761 acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa
     2821 tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag
     2881 ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat
     2941 gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa
     3001 cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac
     3061 aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat
     3121 atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag
     3181 aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag
     3241 cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca
     3301 tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt
     3361 atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg
     3421 cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc
     3481 acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt
     3541 cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg
     3601 attggttact ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg
     3661 cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga
     3721 cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga
     3781 agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct
     3841 tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa
     3901 ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt
     3961 ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac
     4021 cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt
     4081 tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc
     4141 atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt
     4201 aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag
     4261 tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg
     4321 ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga
     4381 agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat
     4441 cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa
     4501 tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg
     4561 aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc
     4621 tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt
     4681 gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa
     4741 tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt
     4801 ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag
     4861 tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt
     4921 actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct
     4981 gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc
     5041 tatcgtttta tcggccgcaa atcaagtctg atgttctttt tagtgattca gatggtaccg
     5101 actatggcag cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag
     5161 tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg
     5221 aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca
     5281 gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt
     5341 caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt
     5401 cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca
     5461 aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta
     5521 ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga
     5581 taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca
     5641 actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta
     5701 tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg
     5761 attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa
     5821 ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg
     5881 acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact
     5941 tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc
     6001 cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat
     6061 ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct
     6121 tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct
     6181 tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag
     6241 agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg
     6301 tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg
     6361 cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa
     6421 gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt
     6481 ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg taatagggta
     6541 gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt
     6601 tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat
     6661 acggacactc ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt
     6721 acaagtaacg catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag
     6781 ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga
     6841 aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa
     6901 gatatccatg cgcattatga cttaggtaac gacttttata aactttggct agatccaact
     6961 ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc
     7021 aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat
     7081 atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca
     7141 actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa
     7201 catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg
     7261 tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt
     7321 tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc
     7381 agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt
     7441 tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt
     7501 gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt
     7561 catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat
     7621 ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat
     7681 ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag
     7741 tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt
     7801 tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa
     7861 taaaagtttg gttctttaat tttta
//
LOCUS       DAZUKA010000129         7811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000129 DAZUKA010000000
VERSION     DAZUKA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.132"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACG8PN_002327"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACG8PN_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4862456.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="ACG8PN_002328"
     CDS             complement(1383..1901)
                     /locus_tag="ACG8PN_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862457.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="ACG8PN_002329"
     CDS             complement(1917..2243)
                     /locus_tag="ACG8PN_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4862458.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="ACG8PN_002330"
     CDS             complement(2256..4235)
                     /locus_tag="ACG8PN_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4862459.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="ACG8PN_002331"
     CDS             complement(4256..4570)
                     /locus_tag="ACG8PN_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4862460.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="ACG8PN_002332"
     CDS             4738..5031
                     /locus_tag="ACG8PN_002332"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4862461.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="ACG8PN_002333"
     CDS             complement(5109..6221)
                     /locus_tag="ACG8PN_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4862462.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6374..7222
                     /locus_tag="ACG8PN_002334"
                     /pseudo
     CDS             6374..7222
                     /locus_tag="ACG8PN_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7575..>7811)
                     /locus_tag="ACG8PN_002335"
     CDS             complement(7575..>7811)
                     /locus_tag="ACG8PN_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4862463.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2500 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
     6241 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
     6301 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
     6361 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
     6421 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
     6481 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
     6541 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
     6601 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
     6661 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
     6721 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
     6781 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
     6841 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
     6901 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
     6961 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
     7021 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
     7081 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
     7141 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
     7201 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
     7261 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
     7321 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
     7381 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
     7441 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
     7501 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
     7561 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
     7621 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
     7681 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
     7741 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
     7801 ggtgtcgcat c
//
LOCUS       DAZUKA010000130         7675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000130 DAZUKA010000000
VERSION     DAZUKA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.133"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACG8PN_002336"
     CDS             <1..106
                     /locus_tag="ACG8PN_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFS4862464.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(175..903)
                     /locus_tag="ACG8PN_002337"
     CDS             complement(175..903)
                     /locus_tag="ACG8PN_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4862465.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(916..2196)
                     /locus_tag="ACG8PN_002338"
     CDS             complement(916..2196)
                     /locus_tag="ACG8PN_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4862466.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2337..2939)
                     /locus_tag="ACG8PN_002339"
     CDS             complement(2337..2939)
                     /locus_tag="ACG8PN_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4862467.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3184..3390
                     /locus_tag="ACG8PN_002340"
     CDS             3184..3390
                     /locus_tag="ACG8PN_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862468.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3702..4421
                     /locus_tag="ACG8PN_002341"
     CDS             3702..4421
                     /locus_tag="ACG8PN_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4862469.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4502..5185)
                     /locus_tag="ACG8PN_002342"
     CDS             complement(4502..5185)
                     /locus_tag="ACG8PN_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4862470.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5404..7590
                     /locus_tag="ACG8PN_002343"
     CDS             5404..7590
                     /locus_tag="ACG8PN_002343"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4862471.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2758 a         1293 c         1446 g         2178 t
ORIGIN      
        1 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
       61 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
      121 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
      181 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
      241 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
      301 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
      361 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
      421 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
      481 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
      541 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
      601 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
      661 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
      721 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
      781 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
      841 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
      901 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
      961 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
     1021 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
     1081 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
     1141 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
     1201 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
     1261 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
     1321 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
     1381 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
     1441 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
     1501 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
     1561 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
     1621 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
     1681 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
     1741 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
     1801 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
     1861 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct
     1921 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
     1981 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
     2041 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
     2101 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
     2161 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
     2221 accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag
     2281 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
     2341 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
     2401 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
     2461 aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
     2521 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
     2581 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
     2641 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
     2701 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
     2761 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
     2821 ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct
     2881 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
     2941 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
     3001 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
     3061 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
     3121 gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt
     3181 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
     3241 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
     3301 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
     3361 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
     3421 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
     3481 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
     3541 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
     3601 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
     3661 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
     3721 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
     3781 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
     3841 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
     3901 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
     3961 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
     4021 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
     4081 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
     4141 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
     4201 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
     4261 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
     4321 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
     4381 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
     4441 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
     4501 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
     4561 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
     4621 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
     4681 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
     4741 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
     4801 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
     4861 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
     4921 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
     4981 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
     5041 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
     5101 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
     5161 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
     5221 cttatcttag cataaacgat tgaaaaaaaa ggaaaaattt tcagaaaact aaaatcatat
     5281 cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata gacaacgaaa
     5341 ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
     5401 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
     5461 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
     5521 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
     5581 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
     5641 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
     5701 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
     5761 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
     5821 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
     5881 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
     5941 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
     6001 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
     6061 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
     6121 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
     6181 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
     6241 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
     6301 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
     6361 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
     6421 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
     6481 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
     6541 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
     6601 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
     6661 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
     6721 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
     6781 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
     6841 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
     6901 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
     6961 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
     7021 ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
     7081 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
     7141 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
     7201 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
     7261 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
     7321 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
     7381 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtaacgacg
     7441 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
     7501 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
     7561 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
     7621 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatt
//
LOCUS       DAZUKA010000131         7627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000131 DAZUKA010000000
VERSION     DAZUKA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.134"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..635)
                     /locus_tag="ACG8PN_002344"
     CDS             complement(111..635)
                     /locus_tag="ACG8PN_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4862472.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG8PN_002345"
     CDS             complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG8PN_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4862473.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG8PN_002346"
     CDS             complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG8PN_002346"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4862474.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2357..3037)
                     /locus_tag="ACG8PN_002347"
     CDS             complement(2357..3037)
                     /locus_tag="ACG8PN_002347"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4862475.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3163..4032
                     /locus_tag="ACG8PN_002348"
     CDS             3163..4032
                     /locus_tag="ACG8PN_002348"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4862476.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4058..4573
                     /locus_tag="ACG8PN_002349"
     CDS             4058..4573
                     /locus_tag="ACG8PN_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4862477.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4620..5183)
                     /locus_tag="ACG8PN_002350"
     CDS             complement(4620..5183)
                     /locus_tag="ACG8PN_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862478.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5238..5417)
                     /locus_tag="ACG8PN_002351"
     CDS             complement(5238..5417)
                     /locus_tag="ACG8PN_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862479.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5494..6003)
                     /locus_tag="ACG8PN_002352"
     CDS             complement(5494..6003)
                     /locus_tag="ACG8PN_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862480.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6099..6479)
                     /locus_tag="ACG8PN_002353"
     CDS             complement(6099..6479)
                     /locus_tag="ACG8PN_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4862481.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6808..7557
                     /gene="pnuC"
                     /locus_tag="ACG8PN_002354"
     CDS             6808..7557
                     /gene="pnuC"
                     /locus_tag="ACG8PN_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4862482.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1582 c         1354 g         2523 t
ORIGIN      
        1 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
       61 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
      121 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
      181 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
      241 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
      301 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
      361 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
      421 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
      481 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
      541 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
      601 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
      661 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
      721 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
      781 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
      841 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
      901 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
      961 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     1021 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     1081 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     1141 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     1201 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     1261 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     1321 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     1381 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     1441 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     1501 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     1561 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     1621 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     1681 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     1741 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     1801 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     1861 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     1921 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     1981 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     2041 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     2101 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     2161 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     2221 gtttttatat gtattatcat acactattcc agaaaaaaat aacagtttat tttatctgta
     2281 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     2341 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     2401 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     2461 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     2521 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     2581 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     2641 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     2701 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     2761 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     2821 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     2881 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     2941 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     3001 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     3061 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     3121 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     3181 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     3241 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     3301 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     3361 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     3421 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     3481 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     3541 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     3601 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     3661 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     3721 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     3781 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     3841 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     3901 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     3961 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     4021 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     4081 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     4141 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     4201 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     4261 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     4321 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     4381 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     4441 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     4501 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     4561 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     4621 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     4681 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     4741 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     4801 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     4861 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     4921 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     4981 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     5041 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     5101 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     5161 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     5221 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     5281 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     5341 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     5401 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     5461 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     5521 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     5581 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     5641 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     5701 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     5761 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     5821 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     5881 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     5941 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     6001 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     6061 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     6121 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     6181 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     6241 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     6301 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     6361 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     6421 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     6481 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     6541 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     6601 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     6661 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta tattgaaaga
     6721 ctagtttttt ttcaagaaaa gcatacaata gattgtgtta gtaaatacag aacacactac
     6781 atttagtgtc ttaaggagtc ctcatacatg aagtcatatt ttcagttttt agctcatcag
     6841 ctaaaaggct ggccgcaaca aaattattat cttttctttt tcagtcttgg ctgccaggtc
     6901 atgactcttg tcaatagtcc catcacttgg ctgtccgtta ttacatttat cggtaccacg
     6961 cttggtgtat tgtgtatttt atccatcaat gcggcaaaat cagtcaacgg tattttaggt
     7021 attttgtctg cgatttgttt tattatcgtt gggttttcag caaagaacta tttaagtatc
     7081 ggtgaacaga ttgcttatat gatcacattg gatatccccg tattgttaag taaagaatgg
     7141 aatcataata tggcttcaaa aatccgaaaa ttcactggaa aaagctggtt tatcgcaatc
     7201 atcagtactt tccttgtcta tcttgtttca ggattcctga ttggtcgtta tacagatgat
     7261 cctcggccgt gggtagatgc cattgctttt tctattagct tgactgctgg tatcatcagt
     7321 ttcttgcgtt acaacaacca atattactgg tggttagctt cagggcttgc tcaaatggtc
     7381 ctttggtata tttcatttac gcatggatct gcaagtttgg cgatgttcgt gaacagctct
     7441 gtttacttgc tgaatgatgt ccttgcattt accgtttcac cttggtataa ccaaaaagaa
     7501 cgtgaacgta tgcaaaaaat ggaagaagaa tactacaacg cacaacaaca cgcataaaaa
     7561 aactgagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
     7621 ggcggaa
//
LOCUS       DAZUKA010000132         7600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000132 DAZUKA010000000
VERSION     DAZUKA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.135"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG8PN_002355"
     CDS             complement(138..428)
                     /locus_tag="ACG8PN_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862483.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG8PN_002356"
     CDS             complement(546..806)
                     /locus_tag="ACG8PN_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862484.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG8PN_002357"
     CDS             complement(943..1356)
                     /locus_tag="ACG8PN_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4862485.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG8PN_002358"
     CDS             complement(1812..2297)
                     /locus_tag="ACG8PN_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862486.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG8PN_002359"
     CDS             complement(2436..2651)
                     /locus_tag="ACG8PN_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4862487.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG8PN_002360"
     CDS             2875..3675
                     /locus_tag="ACG8PN_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4862488.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG8PN_002361"
     CDS             3694..5562
                     /locus_tag="ACG8PN_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4862489.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG8PN_002362"
     CDS             5555..6046
                     /locus_tag="ACG8PN_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4862490.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG8PN_002363"
     CDS             6034..6588
                     /locus_tag="ACG8PN_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4862491.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACG8PN_002364"
     CDS             6606..>7600
                     /locus_tag="ACG8PN_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4862492.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZUKA010000133         7379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000133 DAZUKA010000000
VERSION     DAZUKA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.136"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1296)
                     /locus_tag="ACG8PN_002365"
     CDS             complement(<1..1296)
                     /locus_tag="ACG8PN_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4862493.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVG"
     gene            complement(1416..2063)
                     /locus_tag="ACG8PN_002366"
     CDS             complement(1416..2063)
                     /locus_tag="ACG8PN_002366"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS4862494.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2147..3574)
                     /gene="uxaC"
                     /locus_tag="ACG8PN_002367"
     CDS             complement(2147..3574)
                     /gene="uxaC"
                     /locus_tag="ACG8PN_002367"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4862495.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3648..4727)
                     /gene="uxuA"
                     /locus_tag="ACG8PN_002368"
     CDS             complement(3648..4727)
                     /gene="uxuA"
                     /locus_tag="ACG8PN_002368"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4862496.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4730..6361)
                     /locus_tag="ACG8PN_002369"
     CDS             complement(4730..6361)
                     /locus_tag="ACG8PN_002369"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4862497.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6665..7336
                     /locus_tag="ACG8PN_002370"
     CDS             6665..7336
                     /locus_tag="ACG8PN_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4862498.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2418 a         1474 c         1001 g         2486 t
ORIGIN      
        1 tcctacaatc agaaaacaaa ttgatggaat taatgttgaa gcgttatata acttttctaa
       61 aacatctccc gtttgtacat ttgctaaaga gtcatagcct ataatagaaa gagtccaacc
      121 tccgacgcct ccagctagag cttgacctat ttttctagca aaagaatata ccgcatatac
      181 ggtcccatct tctctttgat cagactttac ttcttgatca tcaataacat cagtaatatt
      241 tgcccaaata acagtattaa acacacccaa acccatatag ccaaccgaac taagaattaa
      301 gaatacatac atattactag cttttatcaa aaataagatc aaatatacta ttgctgaaaa
      361 gaacattcct actgatgcca gttctttttt tccatatttt ttacttaaaa acaaagttaa
      421 aggcgaaaca actattaaaa ccaaaatagg attgataaaa ttcattaaaa ctattccctg
      481 tgcatcatta tagtaatcag gaaatacata attattcata ctcataatta ataattgagc
      541 aaggagtaag aaaatagccg caccaattat agaaagtaat gccctatttt taactaaccc
      601 ttttaaagat tttaacaatg aaactttgta ttgagattgg tcaattctta ctcgttcagt
      661 agttagttta taacatatta aataacaaat aagtgcacat atcgaaaata ttcccgcaac
      721 aattgtaaaa gttgatccac cacgaactat ttgagcgcct gaatcatctc ttgtataaat
      781 aaataatggt gttaatacac caatgaccaa acttgctaaa gtagctccaa catttcggaa
      841 tgtcgatagc attgttcttt cttctgtaat agaagtaata gctgatgcca ttgatccata
      901 aggaatattt atagatgtgt aaaaaatact cccccataat atatacgtaa catacatgta
      961 tactacctta acgctcatag aagctgaaat catagaagtt tgatacatta aaaaacttgc
     1021 taaagcgact ggaccactca ttcttaaaat ccacggttta aacttaccct gcttagttgc
     1081 tttcgatcta tcaacaatac gccccatagt tacatcagta actgcatcga taaatcttgc
     1141 tactaaaaat aatgttccaa cggcagctcc gcttatcccc aatacttttg tataaaagat
     1201 cattaaaaaa gaactagcaa aaatgaaagt aaaatcgttt ccaaaatctc caaacatata
     1261 tcctattttg tctcttatac caaattttct ttccatacaa ttctctccct tctgttgtta
     1321 agcgttttca ttctgatgaa agtacgtact atactctgaa gcgtaaagag tcagtattta
     1381 ttttttacat ttcagagtat tatttgtttt ttctcctaat aattctttat ctcataaaac
     1441 ttatctatat atttactagc tactcgactt acttcttcaa aatcaccttt ttcaacagaa
     1501 gccaataaat tcccacctac accaactgca acaactccta cttcaaacca atcttctaag
     1561 ttctctatac ttactccgcc agtaggcatt atatttacgt gtggtaatgg agcttgatag
     1621 gcttttacaa tggatggacc aaaagcagaa cctgggaata gtttaatgat atctacgcca
     1681 gcagtaagtg cctcctttat ctcagtaatt gtcatacaac ctggtaaata aggaatttga
     1741 tataaattac aaatctcagc tgtttgtcga tcaaaagatg gactaacgat atattctgcc
     1801 ccagccataa ttgcgatacg agcagtaaac gcatctaaaa ccgtaccggc tccgattatt
     1861 acatcttttt gatcagcata ttttttagca atttctttga ttatttcttc agcattagga
     1921 actgtaaaag ttagttcaat ccctttaatt ccacccgcaa taattgaatt tattgcttta
     1981 atggcctcat ccttattttt cccacgtaca accgcaatga ctccagccgc ttctaactta
     2041 cttaaaattt ctactttttt catatctatc tccttccttt atggatacat actttgtcta
     2101 gctgtactaa cttacaaaga ataaatttaa gcttttattt caagtattat tttctacttc
     2161 cgatttcaaa aaaattaaaa taattatacg cattacgata acaaatattc tccactatct
     2221 gactaagata catttcatca tctggaattc ggcctgcttt aacccactcg cttagtaatt
     2281 gacataatac tcgacgaaaa tactcatggc gaggatagct tagaaaactt cgagaatcag
     2341 tcaacatacc tgtaaaattc gacaacaaac tttgttgtgc catcgtagtc aactgttgac
     2401 gcattccttc agctgtatca ttgaaccacc aagcacatcc caattggatt ttttgaatca
     2461 tacccccttg gaagctttgc attgctgttg ctagtggaag ccaatcagtt ggatttaatg
     2521 aatataaaat tgtttttggt aagcacccac tcttttcagc ggtatcgaac aatgctacaa
     2581 cattttgtgt caaactagct tgatccccca tcgaatcacc accgacatct actccaattt
     2641 gttcctttaa tatacttgaa ttatttctta aagcatttaa atgcatttgc atcgtccaac
     2701 catatcttgt atactcagac atcaaatgta attgaatacc tgtaacaaat tgtgcaattt
     2761 ctgataaatg gaaatccgtt tttcctgcta aagcttttat taacagattg tcgagtattt
     2821 tttcatctgc tatttcataa aaataagtat tcaaaccgtg atctgctaat cgacagccaa
     2881 tttgatgaaa atgatccact cgttggctca atgcttgctt caacccatta aagtctttga
     2941 ttggtatagc cgctgtttga ctaagtttta aaatatagtt cgaaaagcct gatgatttga
     3001 tactccagac cttatctgga cggaaagtag gtaatacctt gaaagaatta tcctgtttag
     3061 aaagctcagt atgataccac aagtcatctg taggatcatc cgttgtacag atggctttca
     3121 catccatcat ctccaacaat tctttcgggc gataggaatc cgtttgaatt ttttcgttag
     3181 cacgtcgcca aatttctggt gcattcttct cagaaattgt caaatcaata tcaaagtaac
     3241 gttgtaattc caaatgagac cattcaaaaa taggattacc tggcgccttc tccactgttt
     3301 ttacaaaagc taaaaatttt tcataatcat cacctggacc actgatcaac tcttcttcta
     3361 taccattagc tcgcatcaat cgccatttat aatgatctcc agctccttta tcatttaacc
     3421 atacctgagt caaattcttg tattgcttat tttctagcaa atatgctggg tctaaatgac
     3481 aatgatagtc gataattggt tggtgttttg caaatttatg aaaaagtctt ttagctattt
     3541 cattctctaa taaaaagttc ttatctaaaa acatatttaa acactccttt ccttccgtta
     3601 gtttttagaa gagaaaattt ttcctctaaa taaactccct tatataatta tttactcgcc
     3661 cccttttcaa gcgcttccca aataccattt aaataaactg cacctagagc tcggtcatat
     3721 aatccataac caggtctacc ggtttctccc caaatcatcc gaccatgatc tggacgaata
     3781 tagccattaa atccattatc gtacattgtt tttaatatct tatacatatc tagagaacca
     3841 tattctgatt tatgcggcgc ttcgtaaaaa tcgagtccat tgtcacttaa atatttgata
     3901 ttacgaacat gtgcaaaagg aatccgatcc cgttgacaaa attcagatag aatttcatag
     3961 acatcattat ctggtcgttc tgcaatactt ccagtacata atgtaatacc attagaagga
     4021 gaatccacta cattacacaa ccaatctaaa tcgtcacgat ttttaacaat acgaggtaat
     4081 ccgaaaatgg aatatggtgg atcatctggg tgaactgcca ttttaatccc aacttcttca
     4141 catgtcggga taattgcttt gagaaaatat gcaaagttct ctcgtaattt ctcttcatca
     4201 acttctttat aggcttctaa taattgctta atttgtgata accgatgagg ttcccaaccc
     4261 ggcaattcaa aattattagt agaattttct acttctctaa taatttcctc cggatcagta
     4321 gggatatttt ttgaaatata ggctaacgta gaagacccat catctaaacg ataatccaaa
     4381 tccgatttta cccaatcaaa tactggcata aaattataac agataacctc aatcccaaat
     4441 tctgccaaat tgcgaatcgt ctgtttataa ttctcaatgt attgatctcg tgttggtaaa
     4501 ccaattttga tatcatcatg aacattaaca ctctcaataa ctttcaaggt aagtccattg
     4561 gcttcgactt catcttttaa agatttaatt cgttctctcg gccaaacctc tccggctggt
     4621 atatccatta atgttccaac aattcctttc attgctggga tctgacgaat ttcttgtagt
     4681 gagatttttt cttcatttgc cccataccat ctaaaagtca tttccatagt tactttcctc
     4741 caacttctgt tatatattta ttgattgttt tacgaactgc ttgaggacct actagcattt
     4801 cgcaaaagat tttttcagtt ttctttccta ccccaacttg atataaatct aaaccaaaga
     4861 tttctttatt ttctaaaatt tcctgtaccg cacaatgaat ctttgaagga tcttgttcac
     4921 ctaatttcaa tactgataat ttatcctgca actctaccaa taacggatca ggacttggca
     4981 caaaattttc cccttgatca tcgactgcca gtaaataacg taaccaagca gcaatagcga
     5041 taggaataaa tgttagattt tctgcatttc cgtctttttc gacatacttt ttaattgttt
     5101 caccatagcg aatcgctact ttttgagaag tatctgttgc aattcgttgt ggtgtatctg
     5161 gaatataggg attaggcaaa cgtttattaa ttacttcatc aataaattgt tttggatcaa
     5221 taatgcctgg attttcaact actggtaaac cttctaaata acctattcct ttgattaaac
     5281 caaccaaatc atcatcctgc atctctgctg aaattgattg atatcctagc agacaacctg
     5341 ttactgccaa agcagtatga agtggattca agcttgtcgt aaccttcatg atgtctgctt
     5401 tatcaaccgt ctctctatct gtcataatca caccagcctt ttccaattta ggccggccat
     5461 ttggaaaact atcttcaata actaaataat gaactacctc agtattagtg aaagcagcaa
     5521 tattggtatg tttttttgtg tgaatgatat ctaaatcctc gagccccatc atcaatagat
     5581 cttcctttac attctctgaa ggatttggcg taattcgatc aatcatactc caaggaaaag
     5641 tcactttttc tttatccttt aaataagcaa taaatttttc agaaacatat ccttcttttt
     5701 gccaagcttc tgcaatcgta aaaatactat cttgaaaacg ttgaccattc tgtgagaaat
     5761 tatcagtaga aaccatcgca atcggcaact ctccagcatt aaatctgcta tataacaagg
     5821 atgttacaat actcatcgta tgttgaggat tgctaggtcc agatttgata tcagctttga
     5881 ctaccgaaaa gaattgccct ttagaatctt ttaaactgta acctttttca gtaatagtaa
     5941 acgttgtaaa ctgtaattta ggatcttcaa aatatttaac cactctttga tagtccgcta
     6001 tattcttcgg attgcaatat aaactatcag ccgttactgc caaaagtctt tttttgagtt
     6061 caccatctcc actcataaca atttccaaaa tgtcattgtt atagtcatga taagcttttt
     6121 ccactacttc ttcatcaaat gtttcacaca caactactcc tgtttctaat tcattttgat
     6181 ctgctaagca ttgagcaatt tctgcatgaa atcctcgata aagatttcct ccaccaaaat
     6241 gaagccatac tggatgttgc tggccttctt gcttaacttc tttgacatca aaacacggca
     6301 gtagaatctg catttcattg aattcttcga tagaattttg ccaattttta atcgaaacca
     6361 taattaacct cctcaaactg atatgttagt tttgatacat tttatatttt aaaacccatt
     6421 gtaaccgttg tcaatataaa aagggaataa acttgctttt ttctaccata tcagaatgat
     6481 tgtatttttt attcagaaaa ataaaatact ccttcttttt ggactgattt tatcgaaact
     6541 tattttttct ttttttcatg tattctttta tttttataac tagtgtatta cttcaaaatt
     6601 tgatattata tggcatgaac agaattaaaa tatcagttaa aataaaagtt aaggagcatt
     6661 gtgtatgaca attaaaacta gtctccaaaa tcaagcttac caatctattc gccgacaaat
     6721 tatctatgct gacttagaac ctggaaaaaa agtatcagaa aaaaaattag aagaaacact
     6781 ttctattggt cgtacaccaa tacgtgaagc actgattcaa ctccgacaac aaggtcttgt
     6841 tgatacaatt cctcaaagcg gaaattatat ctcattaatt gatttagatt cggccaaaaa
     6901 tgctcgattt gtcagagaac atttggaacg acaaattatg ttagagtgtt gtgccaaaat
     6961 gaataaccaa aaaaaacgga ttttagaaac tataatttca gaacaagaaa aagctgttag
     7021 tcaaaaaaat gctcgtagtt tttttcataa tgataattta ttccataaag cttgttttga
     7081 aattgcagag cgagaagaag tatggcaatg gttagaaaat aacaatacac acttagaacg
     7141 atttcgatgg ctacgagtaa aaacggaagg attaaagtgg gaaaaaatta tggaacaaca
     7201 ttatcaacta tttgaggcat taacaaaaca agatccagaa gaagctaact ttctaacatc
     7261 gttgcatctt catttaatgc ttaatgagca aaatattgtt ttagctactc atccaaaata
     7321 tttcaaaaat atataacttt taactctttt taaattaaat ggttgttctg atcagaaaa
//
LOCUS       DAZUKA010000134         7114 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000134 DAZUKA010000000
VERSION     DAZUKA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.137"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..759
                     /locus_tag="ACG8PN_002371"
     CDS             130..759
                     /locus_tag="ACG8PN_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862499.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(883..1992)
                     /locus_tag="ACG8PN_002372"
     CDS             complement(883..1992)
                     /locus_tag="ACG8PN_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862500.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2201..2623
                     /gene="mgsA"
                     /locus_tag="ACG8PN_002373"
     CDS             2201..2623
                     /gene="mgsA"
                     /locus_tag="ACG8PN_002373"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4862501.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      2810..2908
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            2946..3482
                     /locus_tag="ACG8PN_002374"
     CDS             2946..3482
                     /locus_tag="ACG8PN_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4862502.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3522..5285)
                     /locus_tag="ACG8PN_002375"
     CDS             complement(3522..5285)
                     /locus_tag="ACG8PN_002375"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862503.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5288..7003)
                     /locus_tag="ACG8PN_002376"
     CDS             complement(5288..7003)
                     /locus_tag="ACG8PN_002376"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862504.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         2128 a         1368 c         1409 g         2209 t
ORIGIN      
        1 attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg
       61 acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag
      121 agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg
      181 atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact
      241 ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct
      301 gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga
      361 gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt
      421 atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt
      481 gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt
      541 ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg
      601 gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga
      661 acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa
      721 gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa
      781 actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga
      841 gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac
      901 ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat
      961 ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt
     1021 ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac
     1081 tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat
     1141 accgaagata acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc
     1201 aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt
     1261 aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc
     1321 ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg
     1381 gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag
     1441 acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt
     1501 tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc
     1561 cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag
     1621 acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg
     1681 taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt
     1741 ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc
     1801 gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga
     1861 ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt
     1921 aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc
     1981 catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta tttaaagtat
     2041 aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc ttttttttgt
     2101 caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg atacactgga
     2161 gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc
     2221 tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga
     2281 aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt
     2341 gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat
     2401 ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca
     2461 tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac
     2521 gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg
     2581 aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa
     2641 aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc
     2701 gatataatac gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt
     2761 aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga
     2821 aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt
     2881 tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc
     2941 taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg
     3001 cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg
     3061 tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag
     3121 gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg
     3181 gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag
     3241 agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat
     3301 ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt
     3361 gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt
     3421 acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat
     3481 aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc tgtgaattgt
     3541 gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg ggtgcccatt
     3601 tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat tgtggacagg
     3661 cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc ttgttggatt
     3721 tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa cataggggga
     3781 ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga tatctttaac
     3841 ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa ttcatacata
     3901 taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt cccaaaacga
     3961 agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat tccaaaactt
     4021 ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt aattgttcct
     4081 tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc ggcccctgtt
     4141 tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa
     4201 cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc tttgatattt
     4261 ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg atcgatcaaa
     4321 tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt
     4381 tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat cacaccaaca
     4441 gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat cgataaataa
     4501 gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt
     4561 tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact agcttcttct
     4621 tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa gcccgagata
     4681 tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa
     4741 ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag tggacttaga
     4801 cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa ttgattgaag
     4861 gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact gacgatattt
     4921 ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc aaatgcatct
     4981 tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg gatgagccat
     5041 tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg gaacagtcga
     5101 ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc
     5161 atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca aatgtctttt
     5221 gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa tttttttact
     5281 ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct gattgagcaa
     5341 gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact aagatatgat
     5401 gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg ccaagataag
     5461 tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt aaagagtcat
     5521 ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt cgctgttttt
     5581 gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca tttggaagct
     5641 gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct ttttctgtgg
     5701 cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt acagaagttt
     5761 ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga acatccattc
     5821 cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat tgtgtcaaag
     5881 tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa
     5941 atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca acatggtcaa
     6001 aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct tcaagaatcg
     6061 aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca cgtgtgaaga
     6121 tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata taattgatca
     6181 aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg ccaactgtca
     6241 gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat agggcagaga
     6301 tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg aacttctctg
     6361 tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga ttctgactaa
     6421 cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga acggtttttt
     6481 tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa aacagaccta
     6541 cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac ggtgctctga
     6601 tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg tttgtcattc
     6661 gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt
     6721 ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact gaagcatagt
     6781 attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc cataagccca
     6841 tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag gaagccatca
     6901 ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg cccaaaatga
     6961 tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc ctcctattat
     7021 ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga aatttagaat
     7081 gaaaagagaa caagcctttt cataaatgaa agtt
//
LOCUS       DAZUKA010000135         6896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000135 DAZUKA010000000
VERSION     DAZUKA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.138"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            484..765
                     /locus_tag="ACG8PN_002377"
     CDS             484..765
                     /locus_tag="ACG8PN_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862505.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            767..1183
                     /locus_tag="ACG8PN_002378"
     CDS             767..1183
                     /locus_tag="ACG8PN_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862506.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1805..2959
                     /locus_tag="ACG8PN_002379"
     CDS             1805..2959
                     /locus_tag="ACG8PN_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS4862507.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2932..3375
                     /locus_tag="ACG8PN_002380"
     CDS             2932..3375
                     /locus_tag="ACG8PN_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862508.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4545..5513
                     /locus_tag="ACG8PN_002381"
     CDS             4545..5513
                     /locus_tag="ACG8PN_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4862509.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5530..6054
                     /locus_tag="ACG8PN_002382"
     CDS             5530..6054
                     /locus_tag="ACG8PN_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4862510.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(6225..>6896)
                     /locus_tag="ACG8PN_002383"
     CDS             complement(6225..>6896)
                     /locus_tag="ACG8PN_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4862511.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT         2532 a         1208 c         1115 g         2041 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaattaataa aaattgctgg ttttattctt ctttttgctt ttgcgagcag agcaacatgg
      181 acccatgttg cgatattttg gtggcgcccc aatccccgtt ttttcgttga atcttactcg
      241 gactgcactc gttttacagc tgatttttct gaattttgaa agagcataag gaaacgtggt
      301 atcactttct tacgaaaggt gctagatagg ctgtgcctat cacgtttctc agaaaaaaat
      361 ttgctatcac ttttttgcca ttgtggctag gctatgtata gtgttttggc gaaaaatagc
      421 tatacaaata ctatatttgt atggtataca taaattatac gctgatacga aaggaaaatt
      481 tccatggaca ttttagttcg aaatatcaat cccaaaatta ttgcaagaat agataaacgt
      541 tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc gaaataaata tctaaaagtt
      601 ctaatcgaaa gtgattttga ccgtcaactt atgaattaca aaaaagatca attcgattta
      661 ttactcgaaa aatttattgc cattcaaaca gaaaatacga acatactgaa tgactatgtt
      721 cacacaaaca accaactcat tgaaacactt atcggaggaa tataacatga gaaaaacttc
      781 tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac gttttagatt ccatcatcga
      841 aaaaaatcca gacgtcaaca attattcaga agccattcgt tttttatgta tgagttatga
      901 acctgatacc aatacaacca caaccagtaa ccaagcaaaa ctcaatgcca tgggaaaaga
      961 aatttctatc atgacagaaa tcatggcaga atttgctgat ttatatctaa aagatactgg
     1021 tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg aaagaaaaaa ttgaaaatca
     1081 actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa aaagaaacca atcaaacctt
     1141 tacttctgga aattttgacc caacaaactt tctaaaaaga taaaaaaaga aaccgatccc
     1201 taaaaaaatt cctcgatttc tttttaggga tcagtcttcc agcaagattt gcaagttggt
     1261 accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact tcttcttttt gtattttgag
     1321 gcgctacccc aatccccaag tatcggttat ttaatagcgc ggaaatccaa agaataggac
     1381 gtaccctgaa acaaaaaaag actgctctca cgagcagccc aaaatgagat tatcctattc
     1441 atcaacataa aaatattaca aagtgaagaa ctagagaaag acaaaaaaag catatcaaat
     1501 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaactaaaaa
     1561 agagaaatcg cgtaaaatca agcattagaa gctagtaact agctagacca attaaaaatc
     1621 acaaagtgag cgtctctatc cattggtaga ggggctactt ggcatagtcg acattatgtt
     1681 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     1741 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     1801 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     1861 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     1921 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     1981 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     2041 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     2101 agtattttca gacgctgata atacgattaa atatatttat accgtcaaaa aaaatacaaa
     2161 tggagaatat tcaggaagta tttcacaatt tttagcaaaa gaacttcaaa atctagtaga
     2221 tagtaatata acagatacaa ccttattttt tcaagataat agcatttatt attccgataa
     2281 tggacaaatt aaactacttt ggaaatcacc tttaagtaca gaaaaaaatg gtgtttcttc
     2341 ctttacagaa aaaaatgatc ttatatccgt aaatataaaa aataaaatta aaaatttctc
     2401 taaaaaacaa aattttcctg cggttaaacg tgtaaaaagt gtctcctccg gagataactt
     2461 tattatgatt aattggcgta tagcagaaac ccaaacaagt tttccgtggt gtgctgctta
     2521 cgtagaagct gctatcttat gtaacaaaac agatgtacgt gctacttcag ctagagccat
     2581 tatgcattat acctatccta atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca
     2641 agatcaaata ataaaatatg caaattactg tggtgtttat cctaaaaaag tagcacgaat
     2701 actaaacctt tcagaggtcc aaacacaact aagaaaagga aatgccattt atatgggaac
     2761 tgctggttat ggaaattata gtaacagccg tcatgcactt gcattatttg ggtgggtcaa
     2821 tcaaggtggt tatcaaactt actatgtgtg gaatccttgg tataactacc caactgtaat
     2881 aagtgcaaat gcaggaccag ttacaattcc tgtacctgga ggtggtttta cttgggacaa
     2941 taccattaca aattggtaaa taaacccatt gctttagtta tgggtctagt gttagttctt
     3001 ttcttaacta gttgttcaaa aaaacaagaa atacaaaaga ctgatctgat tccttttcta
     3061 ggaacttggc aaactaaaga tcattcagaa cgtctagtaa tcactaattt aaatacttca
     3121 aaaaatatag aacaatatac catcttagta aaagagaaca atcataaaat tcaaatcatt
     3181 gataaaaatt taaatataaa tgaacaagtt ttcatttaca aagcaacaaa taatttacta
     3241 acactgcgct ctaataaaaa acaattagat tacactttta gtttggattc tccaaaaaag
     3301 ctcgtattta ttttgacaac gaatgatggg acagaaggga cagctaaacc aattgaattt
     3361 tttaaagctg attgatgaat agttgtttga tattacaaaa caataatttc accgaaggtg
     3421 aaacaagcga aactttacaa aaagtggcta gcatttgcta gccttttttg tattgtggag
     3481 cgtgggtttt gagggggatt caagggggag cttgcgcccc tttgacaagc ggttatttgc
     3541 accaaatacg taggtatttg gctagcaaat atagagcttg ccaaacactt tagtgagttt
     3601 tgtagaaagt acctttaaag gtactttcac attacgaacg atacgtccgt aggacgtact
     3661 gaaagggtgg gatttatgag cgaactaatg caaaatttgg ctagcaaaaa aagttaaaaa
     3721 atatatgcta ataatcgagc gaaatgaaca ctttttcaag tgagcgaatc cgccatgaca
     3781 aagctgaagc agtttatttc agctttgtca tggcgtgtgc ctagactttt aaaaaagtcg
     3841 gcacacgagc aaatatcttt atcaagctct aatttgcgtt ttaagacgtt tgaaacgtct
     3901 aagtatataa atatataaga gtaggtttaa gagtgcttaa atcgaatttt agagggcatt
     3961 cagacaactt tagggttgtt agggcttgcc ctgaccgcct gtaaggcgct ttaagaacaa
     4021 gataagggat ttatactaag actgtcttaa aacgtctcaa acgaaggctc atagcttctc
     4081 aaagagttcc ttatgaacat ttttagcaaa ggagccggaa gcgactgcgc agtataatgt
     4141 gagagcagag cgatcacacc tttttaagcg actgtaggga gtaagattcc cagttgagaa
     4201 aaatgcgcga atctgtttgg agaaaaaaga agtggttttt taggcgggaa attttgtccc
     4261 cctcttttat aatctctttt taaacctctt tttaaacctc tttaccgtcg gctatatcct
     4321 tactctccca agggaatgac gcttttatct acatcgtttt gtctgtttat ctacatcgtt
     4381 ttgtctgttt atctacatcg ttttgtctgt ttatctacat cgttttgtct gtttgtacat
     4441 tgaattagta catcgacgat gttaatataa gatgtagata aacaggcatt ttgtatttca
     4501 taacgtgtat ctcttgtgcg agtaagaaag gagctatttt aaatatgtct aatgaaatcg
     4561 taaagtatca tcatgaatta aatacaattc cgttgcgaaa atttacacca gtcgaaatga
     4621 atttgttctt ttctatcgta tcaagaatgc gtgatgtagg tgataaaaaa gtacaattta
     4681 cattcgagca attaaaagat ttaagcaatt ataaagcaac tgcaaatgtt cgattcatag
     4741 atgatttaga aacaacgtat gacaaattaa tggatttacg ttttggtaga agaagtgctg
     4801 acggtttgca acgtgaacgt tttgttttat ttaatcaatt taaaatcgac ggaaaatctg
     4861 atattccttt tgctgaaata caagttcatg aaaaagcctt acccttactt aataatcttg
     4921 aagaatgggt gcgctattct ctccaacaat tcaatgaatt agaaagcagt tattcaaaaa
     4981 caatgtttcg attactaaaa caatacagaa ccaaaggttt cgcttatttt tcaaaagaag
     5041 actttcatga attacttgat attcctaaaa gttacaacca aggtacgatt gatcaacgtg
     5101 tgttcaatcc aatacgacaa gaactaaccg caatctttaa aggtttaaca atcaaaaaga
     5161 aatacggaaa aggacgggga aagcctgtca ttggctatca atttacattt aaacctgaga
     5221 taaaaaaatc agacgacttt aacaaacatg ccccaacaaa agaagaacaa aacaaagcat
     5281 tagaactagc taaccaacaa gttcaatctg aaaaagcacc tacttcatta caagaaaaaa
     5341 tcaaagaaca aaagcgaaaa ttggaacttt tacaacagtt agaaaagtta gaaagagaac
     5401 aaaataccca aaataaagaa aataatgcac aaaaatcaga gataaattcc gatattcctt
     5461 cagaactaaa aaaagactta ttagacaatc ttcaaaactt attcaaagat tgaggggttt
     5521 ttagatttta tgacagaaaa aacaatcaaa gaacttgctg aagaattagg agttagtaaa
     5581 acagcaatta acaaaaaagt ttctgacgaa aatcgaaaac tttggttttc gaaaatcgga
     5641 aacaagtttg tgattaatga aaccggacaa aaggctataa aatcaatgtt ttttaacgaa
     5701 aacgaaaact caaatcaaca actagttggc gaaaaaacga aaaccgaaaa ccacaaaaac
     5761 gccaaccaag ttcccgaatt agaagtcctt tctctcctag aacaacagtt aaatcaaaaa
     5821 gatatgcaac tacaagaaaa agacgagcaa ataaaacaaa tgcaaaaatt actcgaccac
     5881 caacaacaac tcaccctgca agccaaccaa caaatcgaga aactgcagga acaactacag
     5941 ttgacttata cggaagaatc ttcggaaaat aaatcaacga ctctttctga agaaacagaa
     6001 tcgatagaaa aacaatcaaa ggcggagaaa aaatggtggc aattctggca ataaaaccag
     6061 caatgtgaac tactcaccac ttagctaaac ctgacggttc ttaacgcttg aagtgggagc
     6121 ttcttgggaa tagagcatac ttgatagcgt atttctttac taacagcggt gtctcttatt
     6181 taccaagcta tccccgtagt tcctacggtt cttgatgatt ggatctaagc caatcccata
     6241 gaatgtagtc ttagaccttc ggtcagaata ttgatactag cattgatatc acgatcatga
     6301 tgagtatgac aaacaggaca agtccattct cgaatttcaa gagatttctt gccatcttta
     6361 tgtccgcact ctgaacaaat ctgactagac ggaaaccatt tatctacttt gatgattttt
     6421 cgtccatacc agtcagcctt gtattgtaat ttagtcacaa actttgacca agaaacatca
     6481 gaaatacttt tagctaattt atgatttcgc aacatacctt ttgtgtttaa gtcttcaata
     6541 cagataatat cgtgattttt gatcatttct gtactcaact tattcagaaa atcagtacgt
     6601 tgattcatta ctttttcatg caatctagct actttgcgtt tttgtttttg ataattttta
     6661 gcttcaaata gattggtacc tttattttta gccaataaag cacgcctaga caatttgcgt
     6721 tgttcacgct ttagtttctt ttccattttg gacgtgaatt tattattatc aattttttgt
     6781 ccatcagaaa gaatcgcaaa gtccataata cctaagtcaa taccaattgt ggaattagtt
     6841 ttaggtaatt cgataatttc ttctttacac aacaaagaaa tatagtattt accgct
//
LOCUS       DAZUKA010000136         6701 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000136 DAZUKA010000000
VERSION     DAZUKA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.139"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8PN_002384"
     CDS             <1..435
                     /locus_tag="ACG8PN_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4862512.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8PN_002385"
     tRNA            complement(759..842)
                     /locus_tag="ACG8PN_002385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8PN_002386"
     CDS             1370..1870
                     /locus_tag="ACG8PN_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862513.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8PN_002387"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8PN_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4862514.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8PN_002388"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8PN_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4862515.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8PN_002389"
     CDS             3665..4156
                     /locus_tag="ACG8PN_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4862516.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8PN_002390"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8PN_002390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4862517.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT         2059 a         1334 c         1123 g         2185 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac a
//
LOCUS       DAZUKA010000137         6643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000137 DAZUKA010000000
VERSION     DAZUKA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.140"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8PN_002391"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8PN_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8PN_002392"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8PN_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4862518.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8PN_002393"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8PN_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8PN_002394"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8PN_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4862519.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8PN_002395"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8PN_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4862520.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8PN_002396"
     CDS             3582..4271
                     /locus_tag="ACG8PN_002396"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4862521.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8PN_002397"
     CDS             4285..5787
                     /locus_tag="ACG8PN_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4862522.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8PN_002398"
     CDS             5800..6276
                     /locus_tag="ACG8PN_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4862523.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8PN_002399"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8PN_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAZUKA010000138         6559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000138 DAZUKA010000000
VERSION     DAZUKA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.141"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACG8PN_002400"
     CDS             complement(1273..1938)
                     /locus_tag="ACG8PN_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862524.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACG8PN_002401"
     CDS             2064..4100
                     /locus_tag="ACG8PN_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS4862525.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACG8PN_002402"
     CDS             4104..4742
                     /locus_tag="ACG8PN_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862526.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACG8PN_002403"
     CDS             4770..5198
                     /locus_tag="ACG8PN_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862527.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..>6559
                     /locus_tag="ACG8PN_002404"
     CDS             5655..>6559
                     /locus_tag="ACG8PN_002404"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4862528.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTAS"
BASE COUNT         2247 a          997 c         1215 g         2100 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtag
//
LOCUS       DAZUKA010000139         6522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000139 DAZUKA010000000
VERSION     DAZUKA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.142"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1076
                     /locus_tag="ACG8PN_002405"
     CDS             213..1076
                     /locus_tag="ACG8PN_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4862529.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1205..2827)
                     /locus_tag="ACG8PN_002406"
     CDS             complement(1205..2827)
                     /locus_tag="ACG8PN_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4862530.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3076..3597
                     /locus_tag="ACG8PN_002407"
     CDS             3076..3597
                     /locus_tag="ACG8PN_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4862531.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3783..5513)
                     /gene="spxB"
                     /locus_tag="ACG8PN_002408"
     CDS             complement(3783..5513)
                     /gene="spxB"
                     /locus_tag="ACG8PN_002408"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4862532.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5678..6496
                     /locus_tag="ACG8PN_002409"
     CDS             5678..6496
                     /locus_tag="ACG8PN_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4862533.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
BASE COUNT         1918 a         1193 c         1304 g         2107 t
ORIGIN      
        1 agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
       61 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact
      121 tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa
      181 gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt
      241 gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc
      301 aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca
      361 tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt
      421 tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca
      481 gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat
      541 atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt
      601 tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt
      661 ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc
      721 tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa
      781 agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa
      841 gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg
      901 atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc
      961 ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg
     1021 ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc
     1081 agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa
     1141 agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag
     1201 gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat
     1261 catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg
     1321 ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca
     1381 tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta
     1441 atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc
     1501 caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta
     1561 agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt
     1621 ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac
     1681 ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt
     1741 tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc
     1801 tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt
     1861 ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac
     1921 gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag
     1981 ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt
     2041 tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag
     2101 attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg
     2161 ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat
     2221 tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg
     2281 ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt
     2341 ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt
     2401 tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa
     2461 attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt
     2521 aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt
     2581 caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat
     2641 ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga
     2701 acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt
     2761 taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag
     2821 taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata
     2881 taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga
     2941 atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc
     3001 aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg
     3061 aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga
     3121 attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca
     3181 attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg
     3241 aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc
     3301 gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct
     3361 tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg
     3421 gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt
     3481 ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta
     3541 gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta
     3601 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
     3661 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
     3721 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
     3781 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
     3841 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
     3901 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
     3961 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
     4021 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
     4081 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
     4141 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
     4201 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
     4261 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
     4321 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
     4381 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
     4441 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
     4501 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
     4561 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
     4621 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
     4681 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
     4741 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
     4801 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
     4861 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
     4921 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
     4981 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
     5041 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
     5101 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
     5161 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
     5221 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
     5281 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
     5341 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
     5401 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
     5461 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
     5521 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
     5581 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
     5641 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
     5701 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
     5761 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
     5821 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
     5881 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
     5941 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
     6001 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
     6061 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
     6121 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
     6181 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
     6241 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
     6301 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
     6361 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
     6421 caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
     6481 tgtggcatta ggataaataa aaaaaatata tatgatatat ca
//
LOCUS       DAZUKA010000140         6502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000140 DAZUKA010000000
VERSION     DAZUKA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.143"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..3264
                     /gene="fss3"
                     /locus_tag="ACG8PN_002410"
     CDS             37..3264
                     /gene="fss3"
                     /locus_tag="ACG8PN_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4862534.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3377..3691
                     /locus_tag="ACG8PN_002411"
     CDS             3377..3691
                     /locus_tag="ACG8PN_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4862535.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3704..4078
                     /locus_tag="ACG8PN_002412"
     CDS             3704..4078
                     /locus_tag="ACG8PN_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4862536.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4088..4423
                     /locus_tag="ACG8PN_002413"
     CDS             4088..4423
                     /locus_tag="ACG8PN_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4862537.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4506..5846
                     /locus_tag="ACG8PN_002414"
     CDS             4506..5846
                     /locus_tag="ACG8PN_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4862538.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5924..>6502
                     /locus_tag="ACG8PN_002415"
     CDS             5924..>6502
                     /locus_tag="ACG8PN_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4862539.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTN"
BASE COUNT         2179 a         1092 c         1446 g         1785 t
ORIGIN      
        1 tatttttttg tgcttaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa
       61 ttatgtgtat taactatttt gagtttggct gttcaagcaa ttgttggagc aattgctcct
      121 acgattactt ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta
      181 gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact
      241 gttccgcatt atgcagtgta caacggcttt aaacaagata ttttctgtat tgaaccagga
      301 gtcccgattt acaatgagtt cactcctggc tatgagaaaa acccgttacc agatatgtcc
      361 gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat
      421 attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa
      481 cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaataaa tcaagccatt
      541 gacgattatc agaagaaacc aaccttccat aatagtacgg ctaaaacagt attagggcaa
      601 tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa
      661 aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt tgataatcac cccaaatgcc
      721 aattcaaaag aaagtggtgt gctgacgctt aagaaatctg ctggtaccgg aacacctgtc
      781 gcctataaaa aggctggcca acagacttta atggccgggg cgattgataa acctaatacc
      841 tacacaatca aaattgatgt ggaaacggag ggttctttaa agatcaaaaa agtcgataaa
      901 gaatcgggtg atgttgtacc aggaacggtt ttccatttag actttggaaa aactttacct
      961 gcaaaagacg tgactactga caaagaaggc attgctacat tggatgagat tcctcatggt
     1021 acaaaagtaa ccattaccga aaaatcagta ccggcgcctt atacgattga taccaccccc
     1081 atgactacca ccattaaagc aggcgaaacc atttacgtga cttcaaaaaa cgcacgggaa
     1141 aaagggcaaa tcattctgga taaaagcgga gttgaaacag ggagtgatct ttggaatgac
     1201 aattattcct tagctggtaa tacgtttgcc attcgcaaag acagtccaac cggtgaaatc
     1261 gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa caccaaaaga gattgctaat
     1321 gcgttggaat tgggaaccta ttatgtgacc gaaactaaag ctagtcatgg gttcgtgaat
     1381 acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc aaaccgtagc ccctgttacc
     1441 agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa ccactttgac gaaagaagac
     1501 aaagatactg gggataaagc acaaggtaag gcagtctttg agggtgctga atacaccttg
     1561 tttactgcta aggatggaaa agctgttaaa tggagtgagg cttttaaacc agagttagcg
     1621 aagggaacga aaggttctga tgaaacggtc actttgactt tagacgaaaa gaaccaagtt
     1681 gccgttaaac atttagctat caacgagtat tattggcaag aaacaaaagc gcccgaagga
     1741 tatagccttg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa
     1801 aatgccgtga ttacccgaaa tgttacggca aaagaacaag tgattcgctt tggctttgat
     1861 ttctttaaat ttgctggatc agctgcgggc actaccgaaa ctggcttcaa tgatctgacc
     1921 tttaaagtgt cgccattgga agggaccaat gaaatcacag gtgccgaaga tgaagcgacc
     1981 acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat
     2041 ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta
     2101 gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg tgaagagtga gtatgtcttt
     2161 accattaccg aacaagacca aaaacaacca attaaaacgg tgaccgttcc ttacgagaag
     2221 ctgacgaaca aagcattttc tgttagtttg aaccgtttaa tgctctatga tttgcctgag
     2281 gaagaagata gtttaacttc tcttgcgact tggaaagacg gaaacaaaga attaacttct
     2341 ctggattcta ccgagctagt tgataaattg agttacaatc tacatgaaat caaagaagac
     2401 tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa aagctgccca agaaaaagat
     2461 gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa cgttggcgaa caaagagaaa
     2521 acaggaactt ggaaaattca gcataaatta accgccgaac aagttttaaa taaaactatt
     2581 gtcttattca actatgtgta tgaaaataag gaagcttttg aagcaggcga taagccagta
     2641 gcgaaggatg ttagcttgaa taaccaagcc caaactgtca gttgtacagt ggaacaccat
     2701 gtttctatcc aaacaaaggc ccacctagaa aacggttctc agacctttac tcatggtgat
     2761 gtggtggata tgtttgatga tgtgtcgatt actcatgatg tactggatgg ctcaaaagaa
     2821 gctttcgaaa caattctgta tgctttatta ccggatggta caaacaaaga aatttggaaa
     2881 tctggcaaga ttgattatga agtaaatgat aaagaattca ccaaaactgt gcttgcgaaa
     2941 aaagtagata ccggaaaata tccagaagga accaagttta cttttgcgga aatcaattac
     3001 gataaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaacc
     3061 ttaacaccaa aagaagttcc aaccatacta agtacgccga aacaaccgga aacaccaact
     3121 gttccaagtg gctctcaaga atctagtccc acagtgaaga ccttccctca aaccggggag
     3181 aaaaattcca atgttttact gttcattggt tttactttga tttttgcgac ggcgggctat
     3241 tatttctgga atcgccggaa ctaaggtgat gagatgatac aagtaaatcc taagaaccaa
     3301 cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt tgtatccaaa actaacaatg
     3361 aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca gatatggctg aaacctttgg
     3421 aaagttaagt tatgccggtg aaggagaaat tataacagaa ggatatggac ggaatactac
     3481 ggtgattggt cgcagttatc atttgtattc cagtaagcaa cgggccgatg atattgaggt
     3541 ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa gaccaaccgc taaaagccgt
     3601 gaatccccat ttggttgcca aaggctatga gattgagaat cgtgggttta ctgattacgt
     3661 gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat taacagaagg
     3721 tattgtcgta gattcaggtt taacatttgg gaagttgcgt ttttccgcat tacgccgaga
     3781 agtacggaag caaaatgagg atggaacaat tggtaatgag gtaaaagaac gaacctataa
     3841 tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt atcccagcta atgtaccctt
     3901 acgcgagttt gcttatgatg cagaggtaga gttggtcaat ccaatcgtgg ataccgttgc
     3961 caattatgtt tttcgagaag gaactaccgt taattggttc attaaggcgg atgatttagt
     4021 cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa aatgaaagaa agaaataggt
     4081 gaagattatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat atggaaaagg
     4141 cgagctgact gaggaacaac tcgttaaagc attaacctct gatgaaaaaa tgtttctgat
     4201 tatacatcaa ggtttacttg gagaaaatag cgacagtgaa gaagactctg atgtactaga
     4261 ctggttagga gaagaagagt ctttctttat agttgtcgaa gtaaatgaaa acttatgtcg
     4321 gcaggcagaa tccattttgg aagaaattgg tgtggagatg ccggatgcta ttgaaggctt
     4381 tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg tagcagtaaa tgactagcat
     4441 aactttttga aattgcctaa tgaaatccta ttgggctttt tcgattggag gtggaaacca
     4501 tcaaaatgta caaaggacat cgcatacgcg caggagatca gcacttggtt tatcactttg
     4561 ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat tttcaagagt
     4621 ttagacaatt tgatatttct aagctatcac tttctactat tgaaatcgtt tggtctataa
     4681 aagatttagt atgtctgctt ggaagtctcg ctttttcagg agctatgata ttgctttata
     4741 ttcacttttt tcttgatcat tggcgaagct tgtggcatcg acaaaagcta gctcggatga
     4801 ttctagagaa ccattggtat gaagtgaaac aaactcaaag tgaaggcttt ttcaaagatt
     4861 tgaatagtag ccgaaccaga gagactatca gttacttccc caaaatctgt tatcgaatga
     4921 aagatggatt actgtctatt cgcgttcaaa tttcacttgg gaaataccaa gatcagcttt
     4981 taaaattgga aaagaagtta gaaagcggac tgtattgtga gttagtagaa aaggaactca
     5041 aagactccta tgtggagtat actttactgt atgatatgat cgccaatcga attgggatag
     5101 acgaagtagt ggcagaaaat gggactctgc gattgatgaa aaatcaagta tgggcttatg
     5161 attctttacc ccatatgtta atcgctggtg gcacgggtgg agggaagacc tatttcctcc
     5221 tcaccattat cgaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa
     5281 atgcagattt agctgattta ggtacggtga tgcctcatgt ttattcacaa aaggaagaaa
     5341 tttctgcttg tgtggaagat ttctatgaac gtatgatggc tcgtagcaag gcaatgaaag
     5401 aaatgtctaa ctacaaaaca ggagagaatt atgcctatct tggacttcca cccaactttt
     5461 taatctttga tgaatacgtg gcttatatgg agatgttaac gacgaaagaa agtgcggtga
     5521 ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca atctggtttc tttctgattt
     5581 tggcttgcca aaggccggat gcgaaatatt taggagacgg aatccgcgat caattcaact
     5641 ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc catgatgttt ggggaggtcg
     5701 aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta tgtggataca ggtggcagtg
     5761 tgattagtga gttttatacg ccacttgtac caaaagggta tgatttctta gattcaatta
     5821 gtaaattaga aaagatgaat gattgagtga tcttcaatac tgactttata cttcaagtaa
     5881 cgtataatac ttttatatag taggaagaaa ggggataatt aatatggaaa gtaatgattc
     5941 ttatttagag tatgcaaaag caaataagga taagtttatt tcttatatta tcaaagggaa
     6001 atcccccgaa gttggacgag acgcagtttt tatggcaggg agtcctgggg ctggaaaatc
     6061 agaggttgcc attggattag ctgagaatta tgataaccat gtgattattg atgcggatta
     6121 ctttcgcacc cagtttcctg attataatgg aaaaaactct agtctctttc aaaaagcatc
     6181 ttcttggttg gtagagcagg catttagata tgtcttagat catggctatt cttttattct
     6241 ggatgcaact tttgcaatat taagtgctga aaaaaatatc tcgcgggcgt tgaaaaaaca
     6301 ttttcgagtg acgattttct atgtttatca agaccctaaa atcgcttgga aatttacaca
     6361 agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag gcatttatta acgctttttt
     6421 caaatcaaga gagaacattg caaaagtaaa gagtaaacat ccagatgtat tgcttcatat
     6481 cataataaaa gattatacga at
//
LOCUS       DAZUKA010000141         6455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000141 DAZUKA010000000
VERSION     DAZUKA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.144"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..735
                     /locus_tag="ACG8PN_002416"
     CDS             <1..735
                     /locus_tag="ACG8PN_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4862540.1"
                     /translation="AMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKSTM
                     LRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKLA
                     PIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEMLL
                     FDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLED
                     SRNVRDFFENPNEVRAQQFISKVINH"
     gene            749..1564
                     /locus_tag="ACG8PN_002417"
     CDS             749..1564
                     /locus_tag="ACG8PN_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4862541.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1577..2224
                     /locus_tag="ACG8PN_002418"
     CDS             1577..2224
                     /locus_tag="ACG8PN_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4862542.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2240..2896
                     /locus_tag="ACG8PN_002419"
     CDS             2240..2896
                     /locus_tag="ACG8PN_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4862543.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3058..3879
                     /gene="racE"
                     /locus_tag="ACG8PN_002420"
     CDS             3058..3879
                     /gene="racE"
                     /locus_tag="ACG8PN_002420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4862544.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3882..5237
                     /gene="rph"
                     /locus_tag="ACG8PN_002421"
     CDS             3882..5237
                     /gene="rph"
                     /locus_tag="ACG8PN_002421"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4862545.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5238..5756
                     /locus_tag="ACG8PN_002422"
     CDS             5238..5756
                     /locus_tag="ACG8PN_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4862546.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5863..6363
                     /gene="cbpB"
                     /locus_tag="ACG8PN_002423"
     CDS             5863..6363
                     /gene="cbpB"
                     /locus_tag="ACG8PN_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4862547.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2118 a         1089 c         1430 g         1818 t
ORIGIN      
        1 gcaatgatta aatttgatca cgtggataaa tattatggca agttccacgc attaaaaaat
       61 atcaatctcg aattcgagaa aggagaagtt gtcgttgtca ttggtccttc tggttcagga
      121 aagagcacga tgcttcgctg tatcaatgga ttagagacga tctcttcagg taagctgctg
      181 attaatgata ctgacttgca tgataaaaag acaaaactga cagaagtccg aaaaaacatc
      241 ggcatggttt tccaacattt caatttgtac ccaaataaaa ctgttctgga aaacatcaaa
      301 ttagcaccaa tcaaagtgtt gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa
      361 tttttgaaaa ctgtcaatat gttagataag aaagattctt atccttccat gttatccggg
      421 ggacaacagc aacgggtggc aatcgccaga ggtttagcga tgaatcctga gatgctgctg
      481 tttgatgaac ctacttctgc tttggatccg gaaatgattg gtgatgtatt agacgttatg
      541 aaaaaattgg ctcatgacgg tatgtcaatg attgtcgtga cacatgaaat gggctttgcc
      601 aaagaagtgg cggatcgtgt tatcttcatg gctgatggag aagtattgga agacagcaga
      661 aatgtgagag actttttcga aaatccaaat gaagtccgag cacagcaatt tatcagtaaa
      721 gttatcaatc attgatagga ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt
      781 ccttttaggc attgcttttc ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga
      841 agatatttta gagcgaagca aatctaccaa tgaaatcatc tggggtgtca agtatgatac
      901 tcgattattt ggaatgatgg atattgagag cagaactgta caaggatttg atgtagatat
      961 tgcaaaagca atcactaaaa agatccttgg tgacaatgga aaaacagaat ttgttgaagt
     1021 cacttctaaa actcggattc cattattgaa aaacggaaat atcgatgcaa ttattgcaac
     1081 gatgacgatc acagatgaac gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc
     1141 tggccaagct ttgttggtaa agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc
     1201 ttctacaacg gttctagcag tcaaaggttc tacatcggct gcgaatatcc gacagcatgc
     1261 accagatgca aaaattttag aattagaaaa ctatgcagaa gcatttactg ctttacaatc
     1321 tggtcaggga gacgcgatga ctacggataa tgcgattttg ttagggattg cagatgaaaa
     1381 tccagagtat gagttagttg gtggaacgtt tactaatgag ccttacggga ttgcgatcaa
     1441 caaagggcaa gaaaacttct tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga
     1501 tggcacatac gataaaatct atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga
     1561 gtaaggggga gaggcaatga gcgaattgat acaaacttat ggaccaatct ttgtcgacgg
     1621 gttcaagatc acattgtttt cgagtgtctt agcacttgtg tttagtttga ttatcggaac
     1681 gttaatggca atcttccaat tatcaaagaa taaagtagtg agcgccttag caaaggcata
     1741 tgtagaattt tttagaaaca ttccattatt aatcatcgtg atgttttttt atgttgtcgt
     1801 accgatttat tggtttaatc tagatggatt ccaagcaggt acgattggat taacgatcta
     1861 tacatctgct tttattgctg aaacagtacg ttcagggatt caaaccgtac caaaaggtca
     1921 aacagaagca ggactatctt ctggttttac ctattcagaa atcatgcgtt atattgtttt
     1981 accgcaagca ttcaaaatcg ttatcccgcc attaggcaac caatttatca acctcgtaaa
     2041 aaactcatct attttagcaa tcgtagctgg tttagatttg atgtatcaag gagacttgat
     2101 cgctagtgaa acattcaata ccttcgatac gtatatcatc gttgggttat tttacttagt
     2161 tatcacgcta ccgttatctt acttaatggt ttatcttgag aaaaaatggg cggttcgttc
     2221 atagaaggag gggaaataca tggattttag cggcgctttt tcatggatga atatccgttt
     2281 tttattggaa ggattaaagg ttactgttga agtttcattg ttttcgatca tttttagttt
     2341 cttgatcggt ggtctgacag gtgtgattcg ttttgcgaat atccctttct tttccaaaat
     2401 ccttggtgta gtgattgata ttatacggaa tcttccatta ttattgatca ttttcttcac
     2461 ttactttgcc ttacctcaaa tcggcattca gatgaacatc ttctggtccg ctgtcgcagc
     2521 attaacgatt tttgagtcag ctatgttgtc ggaaattttc cgagctggac tcaatgctgt
     2581 tccaaaagga cagatggaag caggattatc aacgggactg acttatgtag aatcgatgcg
     2641 aacgatcgtg ttaccgcaag catttaaatc aatgatcccg gcaattgtga gccaattgat
     2701 ttcactgatc aaagatactt cattagcagt aatcatttct ttacctgaac tttctcatca
     2761 ggcacgtatt atttatggac agaacaccaa ttatgtgctt ccgatgttca tcatcatgac
     2821 acttatgtac ttcgtggtat gttacgcgct ttctctgctt tccggttact tggaaaagag
     2881 acggtacagt tattaataga tgattctttt agcggttgtg gcaaactaaa gtcacaaccg
     2941 cttttcctct ttccattcga aaaaaagaga tttcgattgt tttaagcaag taagagcact
     3001 tgaaaattca cgctaaaaag tacacaatag ggaggataga atttattgaa aaaaaggatg
     3061 atacgattga cagataatcg ccctatcgga tttattgatt caggtgtcgg cggcttgact
     3121 gtagtaaaag aagccctgaa acaattaccg aatgaaaata ttttatttgt aggagacaca
     3181 gcacgctgcc catatggccc tagacccgcg gaacaggtaa tccagtatac ttgggaaatg
     3241 acggattatc tggtggagca aggaatcaag atgctggtga tcgcctgcaa taccgcaact
     3301 gcggtggctt tagaagaaat caaagctgct ctttctattc cagtcatcgg tgtgatcctt
     3361 cccggtacta gagcggcagt aaaaaaaaca caaaataaac aagttggcat tatcggtacg
     3421 attggtacgg taaaaagtca agcttatgaa aaagcactga aagagaaagt accagaattg
     3481 actgtgacaa gtcttgcttg tcccaaattt gtttcagttg tcgaaagtaa tgaataccat
     3541 tcatcggtgg cgaaaaaaat tgtggcagaa acattagctc ctttaaccac taaaaaaatc
     3601 gatacattga ttttgggatg cacccattat ccattattac gccccatcat tcaaaatgta
     3661 atgggagaaa atgttcaact gatcgattct ggagcagaaa cagtaggtga agtatctatg
     3721 ctgttagatt atttcaattt gagcaattca ccgcaaaatg gtcggacatt atgccagttt
     3781 tatacaactg gctctgccaa acttttcgag gaaatagctg aagactggct tggaatcgga
     3841 cacttaaatg tagaacatat cgaattggga ggaaaataag gatgcgtcat gacgggagaa
     3901 aagcccaaga gcttcgcaaa ctggagcttc aaacaaatgt tttcaaatat ccagaaggat
     3961 cggtagtgat ccgttttggt gatacaacag ttatttgttc tgcaacaata gaaaattctg
     4021 ttcccccttt tttgagagaa acaggaacag gatgggtgac agcagaatat agtatgctgc
     4081 ctcgagcaac gagtacgaga aatcgtagag aaagcagcaa agggaaactt tctggtagaa
     4141 caatggaaat ccagcgtctt attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat
     4201 taggagaacg tagtatcatc gtagactgcg atgtgatcca agcggatggc ggtactagaa
     4261 cagcaagtat cactggggca tttgtggcat tgaaattagc cattgaaaaa cttttaagag
     4321 aaaaagaatt gtcagaagat ccaataaaag aacatcttgc tgcagttagc gtaggaatct
     4381 taccagatgg gacttgcgtc acagatttgg attaccaaga agattccgca gcacttgtag
     4441 acatgaatct tgtaatgaca gaatctggaa aattcgtgga gattcaagga actggagaag
     4501 aagcaacgtt tgatggtgaa cagttgaatg aaatgctctt ttttggaaaa aacgcgattg
     4561 aagatttgat caaagagcaa aaacatgctt tgctaacaga gtttgctcaa aatgatgagc
     4621 ggatagaaga aacaaaaaca atcatcattg cgacaagaaa tccaggaaaa gcagaagagt
     4681 ttcgaaatat gttcaaagaa gcgggatatc atgtaaaaac attacttgac tatcctgaat
     4741 tgccagatgt ggaagaaact ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa
     4801 cgattgcaca attgcttgat cagccagtct tagcagatga ttcaggtttg aaagtggatg
     4861 ctttaggcgg tatgccagga atctattctg cacgttttgc aggggaacaa aaaagtgatg
     4921 cggggaataa cgccaaatta ttatatgaat tgacagatgt gccggatgaa aaaagaacag
     4981 cacaattcca ttgtacgtta gtattcgcgg cacccaaaaa agacagcctt gtagtagaag
     5041 cggaatggcc cggtcgagta gcccgtatac catctggcga aaatggcttt gggtatgatc
     5101 ctttatttat cccagaaggt aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa
     5161 acaagatcag tcatcgggct caagcaatga aaaaattgag tgccgagtgg aaacaatggc
     5221 tggaaggaga gagatagatg cggtatttag tagtaagtga taatcatgga gacagagata
     5281 tcctcgttga cttagtagat cgttatcgtt ctgaagtgga tatctttttc cattgtggtg
     5341 attcggaatt agaagcttct gacccattat gggatacatt ccaagtggtt caaggtaatt
     5401 gtgattatgg accaggattc gaacagaaaa aagtgatcca aactggtcag gatacagtat
     5461 tcatgactca cggtcattta tcgaatgtcc gatttggttt gacacagtta gcaatcgaag
     5521 ctcgagctgc aactgccgat atggtgtttt ttggtcatac ccatcaaata ggctgtgaga
     5581 tggatcagaa aatcctgttt ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc
     5641 aaatcccatc atatgctctt atcgaaagtt tagatgacca aatttctgtt caatactata
     5701 accgtgccca tcaggcagtt gaagagttgg ccttttcttt ttcgagagag cgctgaataa
     5761 aaaaactcta atttttaata aaaataagct ttattttatg aattttgcat ccggtttcgg
     5821 taaaatagta gtgttgaatg aaatagaaat ggaggccatc agatgattgg acctatagta
     5881 agagaaatgc tgctggaaaa tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat
     5941 gttatgtatc atcatccatt gtcccatggt ttacttgtgt tatcgaaggt cggttattcc
     6001 aaaattcctg tattaggaaa agatgatcac tttgtaggac tagtaagttt atcagatgtg
     6061 gtcaataaaa tgattgactt gcaaacaatc agcatggacc cgttagaagg actaacggtt
     6121 gctgatgtga cagaaacaga tgttccgaca attgatgaaa attgggaact agaagaagta
     6181 ctccatttgt tagtcgattc gcctttttta ccggttgtca cagaagatcg gattttcaaa
     6241 ggaatcatta caagaaaaga gctgcttaaa gccgtaaact tcatggtgca tgaattagaa
     6301 cgtcgtaata tattgtctcc gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc
     6361 taaagaacga gaaactaaaa gataatcaaa aaataagtag aaaaaacagc gtaccagttt
     6421 tgactttatc ttgaaaaaga ggctgggaca aaagc
//
LOCUS       DAZUKA010000142         6418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000142 DAZUKA010000000
VERSION     DAZUKA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.145"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2609
                     /locus_tag="ACG8PN_002424"
     CDS             <1..2609
                     /locus_tag="ACG8PN_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS4862548.1"
                     /translation="NLRTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNITYN
                     PIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLK
                     EKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSV
                     YNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMST
                     SRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNN
                     SNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQ
                     FKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGC
                     LQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQ
                     DSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLN
                     VTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQ
                     GGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERL
                     ENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGIT
                     KIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLI
                     NSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDI
                     KVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEI
                     LLPSSLKSYFRL"
     gene            2791..3030
                     /locus_tag="ACG8PN_002425"
     CDS             2791..3030
                     /locus_tag="ACG8PN_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862549.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3046..4419
                     /locus_tag="ACG8PN_002426"
     CDS             3046..4419
                     /locus_tag="ACG8PN_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4862550.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4423..6057
                     /locus_tag="ACG8PN_002427"
     CDS             4423..6057
                     /locus_tag="ACG8PN_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4862551.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6220..>6418
                     /locus_tag="ACG8PN_002428"
     CDS             6220..>6418
                     /locus_tag="ACG8PN_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862552.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2247 a         1049 c         1292 g         1830 t
ORIGIN      
        1 ttaatttgag aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg
       61 cagattctca agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa
      121 acataactta taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag
      181 acaatgatat tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg
      241 gaagtgcaac agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt
      301 atttgaaaga aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata
      361 tttttcttcc cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt
      421 ttcttcaatt acttcaccga agatttggag tagaagatga agttgatatt gatacttctg
      481 tttataatcc ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca
      541 caaaagagcg tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa
      601 gtaattgttt taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta
      661 gagtaaaaag ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac
      721 aatggttagc tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag
      781 ttaccttact tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt
      841 cttgtttgtc tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata
      901 aaaataaaga tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt
      961 ctgtagaatc attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag
     1021 aaggagtatt tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta
     1081 agcaaatacg caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg
     1141 atcattgttg ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac
     1201 aattagcaaa acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt
     1261 tacagattca acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa
     1321 aagaaaatga agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg
     1381 tattaactaa aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg
     1441 cattggttca taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg
     1501 cttctattgt gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa
     1561 ttaacctaca gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt
     1621 tatttggaaa tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa
     1681 agctagcatc aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa
     1741 ctcaaaataa ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat
     1801 tagattctaa ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt
     1861 ctgaagagcg tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata
     1921 tcgaattttc tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc
     1981 tttcttctaa tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag
     2041 ggattacaaa aattgaagca acttttgtcg aagctcaaaa aaaactatgg gctgttttac
     2101 ctgaacattc tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagccc
     2161 tcctaattca agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta
     2221 ttaattcgta cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg
     2281 accgagtgaa aacattgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca
     2341 aacatacgcc cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg
     2401 taaatatttt tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc
     2461 taacctatct ttttaggcaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa
     2521 gaacgaaacg tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt
     2581 cttcactcaa atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc
     2641 accatgttat ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg
     2701 atagcgagca aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt
     2761 caatcaaaca tgaaagaaag gaaaattcta atgactaaaa ataaaatgac actaaaagca
     2821 gaggttcttc tgtatattca agaacatttt tcaaatcaag ccttttttac aaaaccaatt
     2881 tacttggatt tcgaaataag aggggtatct gctggatcta tcgggggtac tcttcaagcg
     2941 ttgaagaacg aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc
     3001 gtgaaaaaat ggtatttagt tcattcatag agaggggaat caagcatgcg tgtggcttgt
     3061 tatgatcgaa cgtctgttta tgaagaagcg aaaattagta atgacgctca atatgatgca
     3121 gtgaaagagt atgttttaga gaaatatgaa caaattcccc aacgattttc agacaaagga
     3181 gaatcagggg aaaaagcgga gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg
     3241 tcagaagaag agggggtgat ctatgtttgg cgttatgatc gattggcacg tcaagccaaa
     3301 atagccttag aatttattga gttgtgccaa caacatcagg ttcaagtgta ttcgatttct
     3361 gaacctcttc ctgcttcttt ctcacacgcg tcaacgaata aactatttgt gcaattattg
     3421 ttcacttttg cagaacaaca acgaaacgtc attattgaga atgttcgtgc cggactttcc
     3481 tataaaaagc aacaacggca atatttgtct tctgcggtac catatgggta tcgtttagtg
     3541 aatgggaaaa tccaacaaga agtacacgaa gcaaggattg ttcgctatat ttttcaactt
     3601 tatttgacca aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagctt
     3661 ttcttccgcg aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg
     3721 tactatggcg aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt
     3781 ctctcgaaag caaccttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa
     3841 aaagatacgt atctgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat
     3901 ctgagttcga agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt
     3961 accgaccgaa gctgccaagg aatttttctt cgggcagaaa gggttgagtc agaagtattg
     4021 gaaaagatac aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaatta
     4081 acgcaacaat tagccgaaaa gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa
     4141 caagaagcac aatggattaa ggcatttgaa gagggcaatt gttcactgga agaactcaag
     4201 caacaactgc aagctcttca aaagccgatg tctacttctt cgtttctttc actccaacag
     4261 tgtgaagcaa aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattatta
     4321 tgtgactatg tttgtcggat tgaggtaaca aaggataaaa cagtttgtgg gatttacttc
     4381 cgacaactga cacaaaatat tttagaaagg aacgaatgat ctatggaaat gatacaaacc
     4441 aaacgagtcg cattgtatat tcgcgtgagt acacaagaac aagctttaga aggacatagt
     4501 attcaagcac aagaacaaaa tggacgacag tttgccgaac ggatgggcta tgaagtggta
     4561 gaagtttatg ccgatgaagg aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa
     4621 cgaatgatga acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc
     4681 cgtctcggac gcaatatgtt agatgtctta caaacagtcg aggaattatt gcgatatgac
     4741 gtaggattac attcgatctc ggaacaattt gacgtgacca cttcgacagg taaattgatg
     4801 ttacaactcc ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa
     4861 atgtcaatgc aaagcttagt gaaagataaa aagcgttatg caggaggccg acgtttaggc
     4921 tatgtcagtg gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg
     4981 aaaattgttc gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc
     5041 aattttttga atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca
     5101 gtcaaagata tcttacgcaa taaaatttat gcagggtatt tagaatatgc gcgctacatc
     5161 aattgggaaa ccaaacgacg gaaggggaag aatccgagac cgattttagt gaaaggaacc
     5221 catgaaccga ttatttcgga agaagattat caaaaagtac aagaacgcct tgctttagaa
     5281 ggaaaacaac ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt
     5341 ccagaatgtg gcgcaccgat ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca
     5401 aagaaacgga ttcgatatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt
     5461 catgccaatt cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag
     5521 atcgttcagc taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg
     5581 cgacaaagcc aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg
     5641 aagactaaaa ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg
     5701 ttaaaagacc gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat
     5761 gaaatcttag ggatctttca acaaggagag ccgattcaag tcaaagatgt ccaacggata
     5821 ttgactagtt tggatcgttt tcttgcccag agtgagaaga agcaaatcaa agccttgtat
     5881 cgtaccttta tagagaaaat aacctttgat cctaaccata aggagatgtt ggaaattacg
     5941 atgaaattca cgcatgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc
     6001 aaaacaaagg atacggctat ttttgtgtta aaaacaccct ttacgttaac gatctagtct
     6061 aggttatatc aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta
     6121 gagaagtagt tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa
     6181 tataattgta tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt
     6241 attttatgtg aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg
     6301 aatactggag gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt
     6361 acaaaaggtg attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DAZUKA010000143         6387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000143 DAZUKA010000000
VERSION     DAZUKA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.146"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..231)
                     /locus_tag="ACG8PN_002429"
     CDS             complement(34..231)
                     /locus_tag="ACG8PN_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862553.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            400..978
                     /locus_tag="ACG8PN_002430"
     CDS             400..978
                     /locus_tag="ACG8PN_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4862554.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            1073..1210
                     /locus_tag="ACG8PN_002431"
     CDS             1073..1210
                     /locus_tag="ACG8PN_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862555.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(2023..2526)
                     /locus_tag="ACG8PN_002432"
     CDS             complement(2023..2526)
                     /locus_tag="ACG8PN_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4862556.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            2885..3910
                     /locus_tag="ACG8PN_002433"
     CDS             2885..3910
                     /locus_tag="ACG8PN_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4862557.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            4233..5111
                     /locus_tag="ACG8PN_002434"
                     /pseudo
     CDS             4233..5111
                     /locus_tag="ACG8PN_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            5587..5703
                     /locus_tag="ACG8PN_002435"
     CDS             5587..5703
                     /locus_tag="ACG8PN_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4862558.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         2141 a          997 c         1159 g         2090 t
ORIGIN      
        1 gttatatcat aatgaagtga atggtatcat acattaataa tagttttttc tatgtgtatt
       61 tcctttgatc cagcgaacaa gccagatgat aagtgcgatt gggattagaa ctttgaccgc
      121 aaaccataaa agcccgctta ggacactgac aaaaagactg atgacgaagc tagctgcaat
      181 tagcactaaa agaaccatga agatatttaa gaacatattt ttttgacgca tacaaaaccc
      241 tcctcttgtt cattctttac tatagaaaaa gtataacagg gattgtttaa aaacacctcc
      301 aactgaagac tgatttttac ttcatctact taaatgatca aacaattgct ttttctctgg
      361 tttatcatct aaaatagagc ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa
      421 gcatttacag atgctgtaat cgcgattatc atgactattt tagttttaga acttcatgaa
      481 cctaacggag acacgtggga ggcgctcgct gcattaggtt accgattttt tatctatatt
      541 gtcagtttta tcattttagc gatttattgg aacaatcatc atcatttatt tcaagttgta
      601 cataagataa atggacgggt cttatgggcg aacagtttct ttgtttttgc gctttcgctt
      661 tttccgtttg caacttcatg gatcagtgag tacatcaata gcctggctcc agaaatgact
      721 tatggttttg ttaccttgac cgctaatatt gcgtattata tcttggcaaa agaacttgtc
      781 aaagcaaata aaggccacga gaaagcattg aaggcgatgt tgaataatta caagaaatcg
      841 tatctatcga ttggtttgaa tgtgttagga attgtcttag ggtatttagt agcgccagta
      901 gccgtgctga tcgcaaacat cgtgattcta gctgcttgga tcatcccaag tcgtaccatc
      961 gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa
     1021 taaataaacg atcaaatatc cgagtgatac tagtattcaa agagggatgc agatggatca
     1081 aaaaagaatt catgtaggac tgattactag ttttgtcacg atgcttattt atttggatca
     1141 aggtcatttg acttggacag tagttggtgt cgtaggattt ttgacattat tcacagcaat
     1201 aaaaaaataa acatctttat gtaatctgat gttctatcta tttaagaaaa gcatgtttac
     1261 agcagtatga ttctgttgta aacgtgtttt tatttgtgga aaatttaatt tttagaaaag
     1321 tgtatcgaaa aatagatagt tagtttttcc acagtggata acttcgtaag agataaaaat
     1381 aaaaaattag tcaaagaagg gcgtatagat ttcttttgat aggagatata caacagaaat
     1441 gaataaatat taacgtgttt tcaaacagta taaaacggtg tggtttttgg aataaaaaaa
     1501 taacatatct cattttacac ttttgttatt ttatacttaa aaaataacga cacagaaaat
     1561 accgtgtctc agatttctct actacttcta aaaactactg tttttttcct ttgaaatgcc
     1621 ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg agcctaaata
     1681 aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa
     1741 ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga
     1801 ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata
     1861 aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga
     1921 ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac
     1981 acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt
     2041 tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat
     2101 ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa
     2161 acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc
     2221 gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt
     2281 agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat
     2341 caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg
     2401 gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt
     2461 gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata
     2521 ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa
     2581 aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg
     2641 ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc
     2701 ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt
     2761 actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga
     2821 caaacgggat tggcatttca tcttggttca gctttttttc caattactag gaaggaggga
     2881 tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat
     2941 gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt
     3001 gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat
     3061 gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt
     3121 ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt
     3181 ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc
     3241 gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc
     3301 actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact
     3361 ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga
     3421 gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt
     3481 cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt
     3541 gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat
     3601 gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat
     3661 ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt
     3721 tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata
     3781 cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt
     3841 aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa
     3901 attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa
     3961 cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa
     4021 tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt ttgtttatga
     4081 tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc ttacagatat
     4141 tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta tgtttatacc
     4201 atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa
     4261 atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca
     4321 aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc
     4381 aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca
     4441 aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa
     4501 gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga
     4561 atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc
     4621 gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt
     4681 acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc
     4741 tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg
     4801 aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca
     4861 cagatacttg gttactatca cccaatctta ctaaatagtt gaaaaaagag tcaaaaatct
     4921 gtctttttgc agccgattat cgattattat cagttgatga acaggacgat agtggagtcc
     4981 cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac
     5041 agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc
     5101 ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa
     5161 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
     5221 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     5281 ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt
     5341 caataggtaa actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt
     5401 aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc
     5461 tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa
     5521 atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc
     5581 gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc
     5641 aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg
     5701 taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat
     5761 aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat
     5821 gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag
     5881 ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca
     5941 attccctttc aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt
     6001 tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt
     6061 tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat
     6121 tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc tcactccatt
     6181 tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa aagttaactt
     6241 taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta ttgctagaat
     6301 aacatcaagg aataataagt tttggttttc agataatagt cgataagttg tgtattggaa
     6361 ttttagatac agtaataaag tattttt
//
LOCUS       DAZUKA010000144         6375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000144 DAZUKA010000000
VERSION     DAZUKA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.147"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..443
                     /locus_tag="ACG8PN_002436"
     CDS             105..443
                     /locus_tag="ACG8PN_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862559.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            529..774
                     /locus_tag="ACG8PN_002437"
     CDS             529..774
                     /locus_tag="ACG8PN_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862560.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            794..2950
                     /locus_tag="ACG8PN_002438"
     CDS             794..2950
                     /locus_tag="ACG8PN_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862561.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2962..3195
                     /locus_tag="ACG8PN_002439"
     CDS             2962..3195
                     /locus_tag="ACG8PN_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4862562.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3185..4051
                     /locus_tag="ACG8PN_002440"
     CDS             3185..4051
                     /locus_tag="ACG8PN_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862563.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4066..6036
                     /locus_tag="ACG8PN_002441"
     CDS             4066..6036
                     /locus_tag="ACG8PN_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862564.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2451 a          876 c         1247 g         1801 t
ORIGIN      
        1 atatcgagac gtctttttct tagttagatc attgacaagt gaacaaataa gatttgtcga
       61 tgatataatt aacttattaa aaatattaaa aaggaggttt ttatatgttc gattctatta
      121 aaaatttggt tagcggaatg acgcctgata tgaaaacttt agtacaagtt gttttgtatt
      181 tcttggcatt cgttttttcg ttgaaaccag gattcactgc aatgaaattt tttggtgatg
      241 caaaatgggg agatggatta aaatatgttg gggcgattat tgcaatcttt attattcctg
      301 taattacaac cgttatgctt tacacaatgg gtactaaaac aggtaatgat ttgaaccaaa
      361 aagctaatat catatcagca cttattccag ttgttggtat ctatctagtt tcaaaatttc
      421 caaaaaatga actgttaggt taaaagtgaa ttgaacaaat ttggataata tgccgatttg
      481 ttcaatttac tttttaccaa aaaactacgc tccaagcgta gtttagaagt gagggaagaa
      541 agtaaatgta agatggtttt tgatagagaa aaaatgttag ctcacgccaa cgaagtatta
      601 atgagcagtc tgaaaggtac cgaactggca aagataatga atatgaatgt taaccagttc
      661 tatgattatc gaaatgggag taaaaagatt gaaaaagcca gacttgaaac gcttattaaa
      721 ttcgaaaaag catatgtata tatgctagat aaacagaaaa gaactattga ctgaaaaaag
      781 gagttttaca ataatggcaa aattcaaaga tattaccgaa tctattaatt atgtggcaga
      841 aacagttcct gtagttgcac tctctttgaa gccacaatta ctcgaagtgt acaaaaaaga
      901 agaaaaagaa gcgaaggcaa aaaaagctaa agcaaaggta tggaaagaat tcgatgcgtt
      961 gctgaccaaa gatttaccaa agaaaaatta tatggtaatt gaagatgtac aaaagctcaa
     1021 cagtgaggga gttatagatg aggatgatgc tattctcgat tctgagaagt atgtcgttct
     1081 attgaagtca agtttgaatt atgtagctga tctaccagaa aaatttaaag agaagctatt
     1141 agcaggtgtc cgttttatgg atagtagtgg tcaattatta gttattccgg atgatcctaa
     1201 tgtgtttgaa tcagaagttg aagaagctac agacttctat agcgcagtag agcaattaga
     1261 agtagctact aatggattag aacgaattgc tgagaccttc aactcttctg aagaagttga
     1321 atttgaagcc gtagcagaaa atgtaccaga tgaaaaagaa gctctttttg tggatgataa
     1381 caaaccaacc gaagcagaaa accaagaagt aagcatagaa gaaacacaaa tacaaacaga
     1441 aacctaccaa caaccaaaaa ttgatgaaaa acccgaatcc caaagtgaca aagaaaatta
     1501 tgtttatgaa gctgaaaaaa tcacatctga aagtgatgta ccgaaaaact tagatgctga
     1561 ttttttagaa aaaaattttg aacagatcat tgctgacaaa tttcaacctg tagttttaga
     1621 tgagattaat gaccctgtcg atttaaacga ggagatgcta tccatacctg agttgaaacc
     1681 attgctgttt ttagctaaag agagtatgaa cgctcgcatc tctattgcta ataataaaat
     1741 caacagtgaa ttaaataaga cgaaggagca attgatcctc aaaggaaagc aaatgttagc
     1801 tgatgagctt ttgcggatag aggaagcaac ctcattaaat ggtcaaaatc agtttacaaa
     1861 tacactatcg gttgctaaag agagctacga agcagaagca tctaaaattg atgatttagt
     1921 gatggaaaaa tccaatatca ttcgtaaaga ttatgatatg gcgaaacagg cttatttgga
     1981 tgctgaacta aagaaactag agcaacggta tgatgctgag catttaccag aagttaaaca
     2041 acaagaagat gaatatagaa aagcaactga agaaagtctt gatcaacagt accaagaagc
     2101 aattgaatct attaaaaacg atgcattgaa acaaagccaa gaagcaaaag aagttgtggt
     2161 ggaacgaatt atcaattcat tgaaaccaga aattgaaaaa tcattaacag acgtgcaaga
     2221 aaaaacttta gagattgtcg aaaaaacaaa gtcagaaaat gaagctgagt tccatgatac
     2281 aaaagagaaa acaacagatg tggttgtcaa atattttgcg aacaaagaag aagttgaaaa
     2341 gcaggtagct aacaacacga aagaattaga aacaaaactt gtagagcaac aaaaaactat
     2401 cgaagaatat aaagatcgaa tgaaagaggt caaagattta gcagagggtt ataaattaaa
     2461 tagcgaaaag ttggcagaac aaagtgaagc tttaagagaa gacttatctc gttcccgaag
     2521 tgatctagct caaatggcag aaaaaacgaa atcattagca gttgctacag aaaatcaatc
     2581 aagttcaaaa cgtgatttta caagtaaaaa gatggctact atcatttctg tcgctgcaat
     2641 ggcttgcgga acgatcttgg gatcgtccta ctttattagt cataataatc aacaagcaca
     2701 ggctcaaact caaacatcta tttcgagagt ggaaaattcg attgggacaa atacgaactc
     2761 tgcttcaaca aatagtagct atgaagcaac accaactaca gagttcaaag ttgggaaaga
     2821 tgttgtcatc agtattgatg ggaaaacaac taaggctgaa gtgatttcta ttgatggaga
     2881 aaaagcagtt gtgaaaactc cagacggaaa tcagtacaaa attcaagctc acttatctga
     2941 taataactaa ggagggctat tatgcgagaa gaaaaaatag tttatccagt aattcaaaac
     3001 aatcaagaaa acatgacctc ttctcagcac ttacaggaag cacagctcgt gtatcaggta
     3061 aatgaactac cacaaacgaa cgagacaatg agcaagtcaa tatctttaat tggcttgctt
     3121 attgttgcaa tagtgataac tgtaggtgtt taccgtaagt tgaacgtgaa aggaagtatt
     3181 tctgatggca gataactcaa actatggatt taaacataac ttacatcaaa aaccttctga
     3241 aacaattgtc agagttgaat ggcttgaaaa gcatgtcaat agcacctatt ctgttacagc
     3301 aaaaagatta attgtatcag ctatagttgt tgcagtcgtt gttcttggat caattgttta
     3361 cttagtttct gatacgatta aactgaaatt tatttttgtt ccaacgttat taatgataat
     3421 tggatttctt attctaatta gccagtatat gtttattgat ccatctagtg gttcattgat
     3481 ttctggttct attagactgt tcaaatcagt agttaattca gcaagagtat ctatggggaa
     3541 aaagaaacca tttcgaggca cagagatttt aaaagtgaaa caagatggaa caatcgtata
     3601 tcgagatggg agtgtagcat taatgttcaa tctagatggg gctacatcac taaccgcttt
     3661 tccagaagaa gttatggctc aggaatcgaa atcaatacaa tatcaaaata ccagagatcg
     3721 ttccttagct gaaatcaaaa tcacttcatc tcaacctcag aatacggaaa gacaaactcg
     3781 aaatatgaaa atacaaaaac gaaatgcaag gagtaacaaa gcagtctttg acataataga
     3841 tcagcaagaa cgatttgtat cagaatatat tgatggtgtg aaaacaacag taatccaata
     3901 tcttatcttg aaatctcaat ctaagatggt attggatgaa ggtgtggaac gtttagaaaa
     3961 ctatacgagt agaggacttt attatagtgt cactcagttg tcagctaaac aaacaaaaga
     4021 aatattatct gatatttatg cactgaaata gaggtgagag attaaatgga aattatgggt
     4081 agatcacaac tattaaattt caaaaaaaag agaaaaacta aaaaagaaca aaaaactaga
     4141 aattcaaagg tagtgaataa tacctttgtt agtgatttaa catctttagt tggttatcaa
     4201 tttacaccat cttacattag gacaggtaag cggtggggaa caattgtgaa attagtgaat
     4261 acttatggaa tgaatagaaa ttatggattt ggttggatgg ttaaagctat tcctgaagtg
     4321 agagtagaag gggtaaaagc ctatctaatt gaagcagata agccagtgtc acaaaaagat
     4381 caacaagaaa taatgcacga gaatgttggg ggatcaacga atagttatga aaataattta
     4441 gaaggaaaaa attcagatgc tgaaattagc aaaactgtta tgcggattat ggatttgaat
     4501 acagcatctt tactagacag catggcaaat aaaattattg attggaagct agaaatccta
     4561 ttagtatccg attctcctaa taaaatcagc gaacagctaa ttaaactcca acggttgtat
     4621 gatgaaaaaa tgtatggatt aaagcttatg tcatatggag gagatcaacg tgatctgttt
     4681 gatagacttt taagtgaacc aatcgggaat gtctatgagt atacgtctat gagttcggat
     4741 tttgcaggaa atgatcatgc aattcgcaaa ggtttagatg atgcgaatgg atgggctatc
     4801 ggcactatgt ctactagcta cgcaagtggt gtagctttta tggcactgga tgattctttt
     4861 acaagtaaag ttttagtggc atcacacaaa aacgctacaa ttaaaggcta tgatgaaaaa
     4921 atctcagcat catcactttg ggggcaattg attgctaata atgcaatggc aaatcataaa
     4981 cgaacttttc atattgtctt aaataaattt gattactttg gtgatccaac tggtagaaca
     5041 tttgttgcca aaccttcgtt aaacagtgta atagaaaagg ttaatttggc tgaagaaggt
     5101 cttaactctt tggagctttt tggtaatcaa aaggacattg gtgatttata tagacggaat
     5161 ctcgataaac taactcagat tttttatttg ttagcaggca gaacattaga ttctgcaact
     5221 agagttttat tgaaaaaaga attaggtaac ttttatataa gtcagaaact ttgggatatt
     5281 aattatgaag aacgttcaaa acttttggtg aacagtagtg atacaatgcc agttcttggg
     5341 gattttatta gctatttgac taatgctgaa acaaaagcca aaaaatctgg tactgaaaaa
     5401 gaagtcgatc gtattattgt attaaaagac acattgagaa cagcattaga cagcttctct
     5461 aggatgttta atcaaagaac gacactgaaa gaaatcggta aaagtgacaa attacagttt
     5521 tactataact taggtgatct aagagcagaa accaatgtta tggaagctca atttttaaat
     5581 acctttgatt tcatcacatc acaagcaaaa aaagatgata ttgttatgat tcacggcttc
     5641 gataagttat ctattgaaac cgttgggcta attaaaaaaa gattaacaat gcttgaagaa
     5701 gatggaattc gtcttgcctt tctatttgat acaatcggtt caggagaaga caatgaatac
     5761 aaagaagata ttgaaacagc tgatatattc aatacagatg gaaaaattta ccaaagtttt
     5821 gaaagtgatt ttaattacac tatcattgga aagatgacca gtggagagtt aatgaagtat
     5881 gaaaaaaaag ttgctcctgc tagaaaagga gaagatgatc aaaaactttc tgttgatttg
     5941 agacagtatt tgacaacagg aagaagtaat aaatttcaga tcaggagacc agtggatctg
     6001 actaataatt ttgttattgg agagtttatt ctttaagttt tatctttgat tttgagtgta
     6061 taaggaaagg ataagaaaaa acatgtatat ttgagactaa aaacagtctt agagaacatg
     6121 gtacgttttg tgaaaagaga actatagaaa tttcataaag tgaaacctta ctgagtgaaa
     6181 taattatagt ggaggttaaa taatgaaacc aaacataaaa attttagatc gaatatttct
     6241 aggtagagac acagaagtaa tcctaattca acatgaagaa ggatttgaag ttagtatagg
     6301 tattcagaaa cttcaaaaac ctcattattg taatcagttg tataaaaact ttactgatga
     6361 agaaaaagcg agagt
//
LOCUS       DAZUKA010000145         6362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000145 DAZUKA010000000
VERSION     DAZUKA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.148"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..368)
                     /locus_tag="ACG8PN_002442"
     CDS             complement(18..368)
                     /locus_tag="ACG8PN_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4862565.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(383..2110)
                     /locus_tag="ACG8PN_002443"
     CDS             complement(383..2110)
                     /locus_tag="ACG8PN_002443"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4862566.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(2324..3250)
                     /gene="trxB"
                     /locus_tag="ACG8PN_002444"
     CDS             complement(2324..3250)
                     /gene="trxB"
                     /locus_tag="ACG8PN_002444"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4862567.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(3395..4828)
                     /locus_tag="ACG8PN_002445"
     CDS             complement(3395..4828)
                     /locus_tag="ACG8PN_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4862568.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(4828..6231)
                     /locus_tag="ACG8PN_002446"
     CDS             complement(4828..6231)
                     /locus_tag="ACG8PN_002446"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4862569.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         1679 a         1479 c         1091 g         2113 t
ORIGIN      
        1 tctatttgca cctatcgtta ttgatgttcc acatgtttga gtgtctggtt caaaatatcc
       61 agtacatgag aatcatcaag actatacaaa atcgcttttc cctcgcgatg agacttgacg
      121 acatgatttt ctcttaataa acgtagttga tgcgatacag cagactgctc catttccact
      181 gcttgggtga ttgctgtcac attttgttcc ccgtctttca gatatagcag aattttaaga
      241 cgagtggggt cactcaacat tttgaataat tgactgactt tctctacttt tgatttttgc
      301 gtgtctggca atccattcac ctccaacttg aaaaaagact gacttctaaa gtttaccact
      361 ttttccatca gtctcaagtt ttttattctt ctgtcatctt tttgataatc gcttcataag
      421 aggctacttt ttcggctgcg tcttctgtag agcttccttt tacaccaata taaaacttga
      481 ttttcggctc agttcctgaa gggcggatag cgatccagcc attttcttcc aatgtatact
      541 tcaatacgtc ggatggcggt gttgttagtt tttctacgtt tccttcacgg tctgttctcg
      601 tcaattcttt aaagtcttct gtttgaataa cttttgctcc ggcaaattct gtcggtgctt
      661 ctttacggaa tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg
      721 tcacagaaat cgttttttct gcgaaatagc cataggtttc aaaaatcgtt tgcaagccgt
      781 catacaacgt tttgccttgt tttttatagt aagctgctac ttctgctaat aaaagcaatg
      841 cttggatcgc gtctttatcg cgaacaaaag gtttaactaa ataaccataa ctttcttcga
      901 atccaaacat aaaagtatgt gaatggtctt cttcaaattg ttggattttt tcagcaatga
      961 atttaaaccc tgtcaacaca ttgaacatag tagcaccgta gtgttccgca attgctgtag
     1021 gcaattcgct ggaaacgatt gatttcaata ccgctgcgtt ttctggtaaa gtgcctcttg
     1081 attgatgtgc ttccaaaata tattgagcaa acaatgctcc gatttggttc ccagtcaata
     1141 cttcataaga accatctggc aaacgaaccg ctgcgcctaa acggtctgca tcaggatctg
     1201 ttgcaatcag caagtcagct ccctcttttt ctccaagtcg tatagcatat tcaaaagctg
     1261 aatgttcttc tggatttggc gatttgaccg tagtgaaatc tggatctgcc acagcttgtt
     1321 cgggaactag tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt
     1381 tccctgttcc atgaagtggc gtgtacacaa gtttcaaatc tttccccatt gtttcaacca
     1441 attcacgatc aatcgtcact gctttgattt cttctagata agcagcgtcc acttcttctc
     1501 cgatgatcgt gatcaacccg ctatgtttag cttcttcgtc agataatact tcgactttta
     1561 gcggattgct gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt
     1621 gtccgccatc ttctccgtag actttatatc cattgtaagc cgctgggttg tgagatgcgg
     1681 ttaccatgat tcccgcaaac gtcttcaaat gacgaactgc aaaagaaagc tctggtgttg
     1741 gacgcagact ttcgaataca taagacggga tattgtgatg tgccagtgtt ttggctgctt
     1801 ccattgcaaa ttctggtgac tgatggcgtg agtcataagc gatagcgaca cctcggcgtt
     1861 ttgtttcttc accttgtgta tccatgaaac gtgctagtcc ttctgttgct tgtcttaccg
     1921 tatagatgtt cattcgattg atcccaggtc ctaagattcc ccgcatgcct gcagtaccga
     1981 attccaacgg tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta
     2041 attgtttttt taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt
     2101 cccaagacat gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca
     2161 cttattagat atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta
     2221 tcaagcatga ttgcaagtat tcacataaat cggattcagt ggatttcacg cgaaaaaaaa
     2281 gagtaaaaga acaattgaaa ccgttctttc actcttttta aacttatgct gttgtttctt
     2341 ctaaagactc caagtattta tacacttgtt gtccagcaat accgccgtca ccaacagctg
     2401 tagtgatttg tcgcaaatct ttttcacgaa catctccgat ggcaaaaacg cctggcatat
     2461 tcgttttcat ttcgttgtct gttggaatcc agccagcttc gttcgtcaaa cctgcttctc
     2521 taaaaggtgc agttaatgga tccagtccaa catagatgaa tactccgtct gctgctaaat
     2581 caaattcatt gccggttttg acattacgtg ctttcacacc tgtcactacc atttcgtttc
     2641 caacgatttc gtcaacgact gtatcccaca taaaggaaat tttttcattt gcaaatgcac
     2701 ggtcttggat gatcttttgt gcacgtaatt catctctacg gtggacgatc actacttcag
     2761 aagcaaattg tgtcaaatag atggcttctt ctactgctga gtccccaccc ccgatgacta
     2821 ctagacgttt gttacggaaa aacgcaccat cacagactgc acagtaagaa acgcctcgtc
     2881 cagcaaattc ttcctcgcca ggtacgccta atttacggtg gatacagcct gttgcgataa
     2941 tgactgtttt tccttcgtaa cttttatctt cacagatgac ttctttatgg ctgccgtgat
     3001 ccttgatatc ttgaacgatt ccataagcat tttctgtccc aaatcttgag acactttcat
     3061 acatcttata agctagatcg gggcccatga tcgaatcgaa tccaggataa ttttcgattt
     3121 ctgccgtatt gttcatttgg ccgccagggg caccttgttc gatcatcaat acggataagt
     3181 ttgaacgtga agcatacaaa gcagcagtca tcccagcagg acctgcgcca ataacgatta
     3241 catcatacat atgtagattc tcctttactt aatcaagtaa gcatacttta cagccaatgc
     3301 gaaaaaaagt ctattgttct gcttgcgttt ctcgaatagc tttattatac tcgaaaaatc
     3361 cgctgaaaaa caatagactt ttataaatca ttgatcaatt gttcgaaaga ttttctccat
     3421 ttgaatcaat tactttttta taccagttga atgatttttt ctttgaacgg gctaatgtgc
     3481 catttccttg gttatcacga tcgacataaa tgaatccgta ccttttcttc atctcaccgg
     3541 taccagctga tactaaatcg atacatcccc aagtcgtata gcctaataga tcgactccgt
     3601 ctagttctac tgcatccttc atcgcctgga tatgcgctgc caggtaatcg attcggtaat
     3661 catcttccac ataaccattt tcatctggcg tatcgactgc acctaagccg ttttctacaa
     3721 taaataatgg tttttgataa cgatcataaa tatcattcat cgttattctc aagcctaatg
     3781 gatcaatttg ccacccccat tcacttgctt gcagatatgg atttttgaca gaagcaaaga
     3841 tatttcctgc agtctgttcc aagagttcgt tgtcggtagt cgaaactcgt gaagaataat
     3901 aagaaaaaga gataaaatca acagtatgtt ctttcagcaa tgcggcatcc cctggttcca
     3961 tttcgatctg aatgccattt cgctccattt ctttcaatgc ataagcggga tattctcctc
     4021 gtgactggac atcaatgaag aaataatttt ctcgatctga ttttctagaa gcccaaacat
     4081 cttcaggatt gcaagtgtat ggataatatg ctcctgctgc cagcatacag cctactttgt
     4141 tctccggatc tacttcatga gcaatcttcg ttgcgatggc acttgcaacc aactcatgat
     4201 gtgccgcttg atatttgatt tgttcttcat tttctccttc ttcaaaatac agccctgcgc
     4261 ccataaatgg tgcatgaagg atcatattga tctcattgaa tgtcagccaa tatttgacaa
     4321 gtcctttata gcgattgaag atgactcgac acagattttc ataaaaacca acgagttctc
     4381 tggaacgcca cgcaccatat tctttgatca aatgcattgg acaatcaaaa tgcgtaatcg
     4441 tcacaagtgg ctcaattccg tgcttttggc attctttaaa gacatcctca taaaatttca
     4501 agccagcttc attcggctct ttctcatccc ccattggaaa aatacgactc caggcgatag
     4561 acattcgata agtcttgaac cccatttctg caaacaaagc aatatcttct ttataacgat
     4621 gatacatatc gattgcaatt tttgctggat aaaaatgatc ctcatcaaat tcaaacattt
     4681 ttttctttcc ggtgataaca ggaaaacggt cttttccgat tggcacaaca tctacgtttg
     4741 ctagtccgcg tccaccttca tcataagcgc cctcacattg attggctgct gtagcaccgc
     4801 cccataaaaa atcttttctg aaccccatta tacttcctcc attatatgtg tattacctga
     4861 taattctctt gccagttcat cttgaacttc gattccttct tcttttaatc ggctgattgt
     4921 ttccttgaat tgcggtaaat actgctggtg tgcaataaac aactcatcca tgatccgttt
     4981 tgctgtcttt cctgatcgta taagtggatt gatcgtaaac gcctgtaaag ctgtcccata
     5041 atcaccagtc acagctgctt cgatcgtcag caattccata ttcttcatta cttgcaacca
     5101 gcctctttca gcagcaggaa gagaaccaaa cgctacattt ctcgctcctt gtgcgccaac
     5161 ataggcagtg acttctacaa cacaatctgc cgacaggtca ggaacagctc catcattctt
     5221 tgtagataca acgatctgtg tttctttatt tccatagatt gaagcaatcg tttcacaggc
     5281 agcgtctgaa taataagcgc cacctcgttt ttgcaattgt tcaggtttat ggtctaattc
     5341 tggatctttg tataattcaa atagctctgc ttctgtttct ttcacttgct gcgctcttgt
     5401 accaatcgtt tgatattctt ctaaagcatg ttccagcatt tcttcttcac gatagtaata
     5461 gcggtggtag ccacaaggga tcatgttcat ttgttttaat tgttctttga aaaacgggat
     5521 atcaaagata tttttcggta atccattatc ttcatcgtat aaacgatcaa tcaattctgc
     5581 cgtgcggtca tttcctttat tatctgctac tttgtgccaa tggaaatgat ttagaccggc
     5641 aaacttgtag atcagctctt cttttttctt gccaagcatt gctggttctg tcatcatcgc
     5701 catcactggc acattgcata atccaatcac tttatcccat ttgccatatc tgataactgc
     5761 ttctgtcacc atcccacttg ggttcgcgaa attgatcagc caagcatttg gacataaacg
     5821 cttcatgtcc tcaacgattg ccaaaacgac tggaacggta cgaaaagctt tgaatatccc
     5881 gccagcacca tttgtttctt gcccaatcat cccataataa ctagggatcc gctcgtcttt
     5941 gattcgtgca tctaattgac cgacacgaaa ctgagtcgta acaaaatccg catctttcaa
     6001 tgcttcttcc cgatccagag ttgcatagat cttgacttca tatggagaag catcccacat
     6061 acgctgagac atctgactaa cgatctcaag cttttctttt ccttcttcta catctacgag
     6121 ccagatttct cgtatcggta attcctcata tcttttgata aatccttcca tcaactcagg
     6181 ggtgtaactg ctgcctccac caatcgtagc gattttgatt ccttttgcca tgttctcact
     6241 cctttacttt tttacacttt cattatcttt tttgtaaaca aaacaaacaa tagaaatccg
     6301 tttctttcca gtaacattta caaaagttac tgttttctag tggatttttg taaatatctt
     6361 gt
//
LOCUS       DAZUKA010000146         6303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000146 DAZUKA010000000
VERSION     DAZUKA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.149"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG8PN_002447"
     CDS             334..714
                     /locus_tag="ACG8PN_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4862570.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG8PN_002448"
     CDS             696..1610
                     /locus_tag="ACG8PN_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4862571.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG8PN_002449"
     CDS             1855..2544
                     /locus_tag="ACG8PN_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862572.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG8PN_002450"
     CDS             complement(2665..2751)
                     /locus_tag="ACG8PN_002450"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4862573.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG8PN_002451"
     CDS             3427..4377
                     /locus_tag="ACG8PN_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4862574.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG8PN_002452"
     CDS             4398..4931
                     /locus_tag="ACG8PN_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4862575.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG8PN_002453"
     CDS             4947..5117
                     /locus_tag="ACG8PN_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862576.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG8PN_002454"
     CDS             5567..6100
                     /locus_tag="ACG8PN_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862577.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG8PN_002455"
     CDS             6112..>6303
                     /locus_tag="ACG8PN_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862578.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZUKA010000147         6303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000147 DAZUKA010000000
VERSION     DAZUKA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.150"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACG8PN_002456"
     CDS             <1..177
                     /locus_tag="ACG8PN_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862579.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACG8PN_002457"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACG8PN_002457"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4862580.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACG8PN_002458"
     CDS             1199..1771
                     /locus_tag="ACG8PN_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4862581.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACG8PN_002459"
     CDS             complement(2023..2280)
                     /locus_tag="ACG8PN_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862582.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACG8PN_002460"
     CDS             complement(2406..3221)
                     /locus_tag="ACG8PN_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862583.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACG8PN_002461"
     CDS             3738..5063
                     /locus_tag="ACG8PN_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4862584.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACG8PN_002462"
     CDS             5056..5406
                     /locus_tag="ACG8PN_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862585.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACG8PN_002463"
     CDS             5388..5573
                     /locus_tag="ACG8PN_002463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862586.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         2163 a          971 c         1165 g         2004 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 tta
//
LOCUS       DAZUKA010000148         6277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000148 DAZUKA010000000
VERSION     DAZUKA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.151"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8PN_002464"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8PN_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4862587.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACG8PN_002465"
     CDS             499..861
                     /locus_tag="ACG8PN_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4862588.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACG8PN_002466"
     CDS             913..2595
                     /locus_tag="ACG8PN_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4862589.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACG8PN_002467"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACG8PN_002467"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4862590.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8PN_002468"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8PN_002468"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4862591.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8PN_002469"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8PN_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4862592.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2086 a         1093 c         1432 g         1666 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaaggatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaa
//
LOCUS       DAZUKA010000149         6236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000149 DAZUKA010000000
VERSION     DAZUKA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.152"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..270
                     /locus_tag="ACG8PN_002470"
     CDS             <1..270
                     /locus_tag="ACG8PN_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862593.1"
                     /translation="VKDIALDLNYTLAKMNSVIQQAESFCERYPEYKLSFLSENKMIK
                     VEFSSQFLLSKVYSILLEGTIGYILLDSLYKGTYQSLENLSQKII"
     gene            402..1430
                     /locus_tag="ACG8PN_002471"
     CDS             402..1430
                     /locus_tag="ACG8PN_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862594.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1456..1896)
                     /locus_tag="ACG8PN_002472"
     CDS             complement(1456..1896)
                     /locus_tag="ACG8PN_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4862595.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2056..2562
                     /locus_tag="ACG8PN_002473"
     CDS             2056..2562
                     /locus_tag="ACG8PN_002473"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4862596.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2730..4643
                     /locus_tag="ACG8PN_002474"
     CDS             2730..4643
                     /locus_tag="ACG8PN_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4862597.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4648..5649
                     /locus_tag="ACG8PN_002475"
     CDS             4648..5649
                     /locus_tag="ACG8PN_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4862598.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5851..6057)
                     /locus_tag="ACG8PN_002476"
     CDS             complement(5851..6057)
                     /locus_tag="ACG8PN_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4862599.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2287 a          950 c         1241 g         1758 t
ORIGIN      
        1 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
       61 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
      121 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
      181 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
      241 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
      301 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
      361 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
      421 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
      481 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
      541 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
      601 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
      661 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
      721 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
      781 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
      841 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
      901 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
      961 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     1021 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     1081 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     1141 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     1201 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     1261 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     1321 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     1381 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     1441 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     1501 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     1561 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     1621 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     1681 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     1741 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     1801 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     1861 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     1921 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     1981 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     2041 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     2101 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     2161 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     2221 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     2281 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     2341 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     2401 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     2461 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     2521 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     2581 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     2641 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     2701 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     2761 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     2821 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     2881 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     2941 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     3001 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     3061 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     3121 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     3181 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     3241 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     3301 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     3361 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     3421 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     3481 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     3541 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     3601 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     3661 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     3721 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     3781 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     3841 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     3901 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     3961 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     4021 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     4081 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     4141 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     4201 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     4261 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     4321 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     4381 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     4441 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     4501 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     4561 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     4621 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     4681 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
     4741 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
     4801 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
     4861 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
     4921 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
     4981 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
     5041 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
     5101 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
     5161 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
     5221 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
     5281 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
     5341 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
     5401 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
     5461 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
     5521 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
     5581 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
     5641 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
     5701 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
     5761 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
     5821 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
     5881 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
     5941 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
     6001 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
     6061 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
     6121 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
     6181 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctag
//
LOCUS       DAZUKA010000150         6232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000150 DAZUKA010000000
VERSION     DAZUKA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.153"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..561
                     /locus_tag="ACG8PN_002477"
     CDS             <1..561
                     /locus_tag="ACG8PN_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4862600.1"
                     /translation="PVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKI
                     DYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNS
                     KYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKE
                     DYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            596..1363
                     /locus_tag="ACG8PN_002478"
     CDS             596..1363
                     /locus_tag="ACG8PN_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4862601.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1555..1953
                     /locus_tag="ACG8PN_002479"
     CDS             1555..1953
                     /locus_tag="ACG8PN_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4862602.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2260..2634)
                     /locus_tag="ACG8PN_002480"
     CDS             complement(2260..2634)
                     /locus_tag="ACG8PN_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862603.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2656..3006)
                     /locus_tag="ACG8PN_002481"
     CDS             complement(2656..3006)
                     /locus_tag="ACG8PN_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862604.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(3023..3418)
                     /locus_tag="ACG8PN_002482"
     CDS             complement(3023..3418)
                     /locus_tag="ACG8PN_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4862605.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3485..3670)
                     /locus_tag="ACG8PN_002483"
     CDS             complement(3485..3670)
                     /locus_tag="ACG8PN_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4862606.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3752..4006)
                     /locus_tag="ACG8PN_002484"
     CDS             complement(3752..4006)
                     /locus_tag="ACG8PN_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862607.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(4028..4273)
                     /locus_tag="ACG8PN_002485"
     CDS             complement(4028..4273)
                     /locus_tag="ACG8PN_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862608.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4280..4564)
                     /locus_tag="ACG8PN_002486"
     CDS             complement(4280..4564)
                     /locus_tag="ACG8PN_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862609.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4667..4849)
                     /locus_tag="ACG8PN_002487"
     CDS             complement(4667..4849)
                     /locus_tag="ACG8PN_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4862610.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(5055..5540)
                     /locus_tag="ACG8PN_002488"
     CDS             complement(5055..5540)
                     /locus_tag="ACG8PN_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862611.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVDTKVTKIYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(5707..5973)
                     /locus_tag="ACG8PN_002489"
     CDS             complement(5707..5973)
                     /locus_tag="ACG8PN_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4862612.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5975..6196)
                     /gene="relB"
                     /locus_tag="ACG8PN_002490"
     CDS             complement(5975..6196)
                     /gene="relB"
                     /locus_tag="ACG8PN_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4862613.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
BASE COUNT         2000 a         1032 c         1063 g         2137 t
ORIGIN      
        1 cctgttttct ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct
       61 tttcgagact ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact
      121 gattttaaaa ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc
      181 ttgcttacac gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct
      241 tatttaaaag gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg
      301 aatagcaaat acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc
      361 gatcttaaac ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc
      421 aaagatagag gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag
      481 gattacacaa taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct
      541 tatgtgaacc tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa
      601 aaaagatgaa ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga
      661 tttaaagtcg tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa
      721 gaaaacaggc gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca
      781 aaatcttgaa acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga
      841 aatttcgaat ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat
      901 tcattgggat ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt
      961 aattgtctca atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca
     1021 aaaaaaatat agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat
     1081 cgccaaaaat aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat
     1141 agttaaaatt cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga
     1201 tagacgtgtt ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga
     1261 ttttgctaaa gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag
     1321 tgagtttgat gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac
     1381 tcgtttgaag atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta
     1441 atctccttta gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct
     1501 tttttttgaa acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa
     1561 tacttttttt gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat
     1621 tttccagact tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa
     1681 gtattggaag gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg
     1741 cctgttcaag agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta
     1801 aaagctaata cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg
     1861 actgtggaac actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct
     1921 ttgaatgatt tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa
     1981 aaacagcttg ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa
     2041 aagctctcag aagccttctg agagctttta aattgctatg tattgtactg catctgctca
     2101 aattgatttt cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata
     2161 tgtattgagt aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat
     2221 gtgttaggat aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt
     2281 tttatcggta ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat
     2341 ccctgcatcg ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat
     2401 caaaatggtg tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga
     2461 cttataaaaa agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta
     2521 tttccaaaga tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc
     2581 tgctaattga tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct
     2641 cttctcctgt ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc
     2701 ggtttgaaat tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga
     2761 ttcacttaat gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt
     2821 atagcagaat tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa
     2881 gttattatcc tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc
     2941 agttgtatct tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc
     3001 tttcataggt tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa
     3061 gtagttgaga gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac
     3121 taacattttt tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt
     3181 ggatgattac taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag
     3241 gaaattctgc atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat
     3301 atgcttcttc aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt
     3361 tataagcttc tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt
     3421 cctcgctcct tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag
     3481 gctgttaaag ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat
     3541 tgtggatcgg aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc
     3601 cagtttgtcc agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg
     3661 tcttaggcat agctaccctc cctttacaaa atctattata cacattgttg tggatggaat
     3721 caatagaaaa agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag
     3781 caaattgaat gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat
     3841 aggtatctaa gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt
     3901 tctccaagtc ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta
     3961 tgaaaacata ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt
     4021 tcttgtacta agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata
     4081 ggacgtgacc gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa
     4141 ggctagtacc tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg
     4201 gatcaatgcg agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt
     4261 ccaaagtcca cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt
     4321 caatagtcac ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca
     4381 agttcccgat gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca
     4441 cttgccgact gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg
     4501 cattaattgt ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta
     4561 gcatcttgtc actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc
     4621 ggtgtgtata atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa
     4681 acgataaagt aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg
     4741 ctttgcttgt gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc
     4801 cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc
     4861 cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat
     4921 ggtgatgttt ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt
     4981 acctaatacg tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt
     5041 tatgtattta agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga
     5101 gtgagtgatc catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca
     5161 gcgacatacg atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt
     5221 tttggtttat tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga
     5281 tcaccgacaa agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca
     5341 tttaagaagt agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag
     5401 gttccttctg catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg
     5461 atttccaaac catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa
     5521 taatcaccat accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca
     5581 cttatattat acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa
     5641 gtgggatcgt aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct
     5701 tgtctgttac ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc
     5761 atgctcgcta atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga
     5821 tttattagct gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt
     5881 ctcgatccag tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg
     5941 agcttcacgt tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat
     6001 cttcgtggga aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg
     6061 caaagtctac taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt
     6121 tatctccgtt gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa
     6181 tacttattac tggcatagtt tatcaactcc ttttcaatta cattgtacta cg
//
LOCUS       DAZUKA010000151         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000151 DAZUKA010000000
VERSION     DAZUKA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.154"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8PN_002491"
     CDS             725..811
                     /locus_tag="ACG8PN_002491"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4862614.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8PN_002492"
     CDS             complement(932..1621)
                     /locus_tag="ACG8PN_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862615.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACG8PN_002493"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8PN_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4862616.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8PN_002494"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8PN_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4862617.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8PN_002495"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8PN_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4862618.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8PN_002496"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8PN_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4862619.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8PN_002497"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8PN_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862620.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8PN_002498"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8PN_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4862621.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8PN_002499"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8PN_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4862622.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUKA010000152         6127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000152 DAZUKA010000000
VERSION     DAZUKA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.155"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..852
                     /locus_tag="ACG8PN_002500"
     CDS             <1..852
                     /locus_tag="ACG8PN_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4862623.1"
                     /translation="EKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAINR
                     EFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVI
                     KNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGN
                     WKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMG
                     NWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEK
                     VLKKIAL"
     gene            872..1432
                     /locus_tag="ACG8PN_002501"
     CDS             872..1432
                     /locus_tag="ACG8PN_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFS4862624.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2321..3088
                     /locus_tag="ACG8PN_002502"
     CDS             2321..3088
                     /locus_tag="ACG8PN_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS4862625.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3114..3659
                     /locus_tag="ACG8PN_002503"
     CDS             3114..3659
                     /locus_tag="ACG8PN_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862626.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3660..4226
                     /locus_tag="ACG8PN_002504"
     CDS             3660..4226
                     /locus_tag="ACG8PN_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862627.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4241..4489
                     /locus_tag="ACG8PN_002505"
     CDS             4241..4489
                     /locus_tag="ACG8PN_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862628.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4508..4747
                     /locus_tag="ACG8PN_002506"
     CDS             4508..4747
                     /locus_tag="ACG8PN_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862629.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4764..5348
                     /locus_tag="ACG8PN_002507"
     CDS             4764..5348
                     /locus_tag="ACG8PN_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862630.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5455..5736
                     /locus_tag="ACG8PN_002508"
     CDS             5455..5736
                     /locus_tag="ACG8PN_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4862631.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5726..6067
                     /locus_tag="ACG8PN_002509"
     CDS             5726..6067
                     /locus_tag="ACG8PN_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4862632.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2293 a          800 c         1273 g         1761 t
ORIGIN      
        1 gaaaaattaa ttaaaaagga tgagattata atggggttac tggttccgag agctaatact
       61 tatactatac ctttaacgaa acgccaatta attgctaaaa gatatcaaag gattactaga
      121 gccatcaata gagagttttg gaattctgaa agtgatactg ctcacagttt atacgtaggt
      181 tcttatggga gaggaacggc aattagtact agtgatatag atataatagt tgaattaccg
      241 atggcagaat ttgacagatt caaaaattat ttatcaaatg gcccgtctaa gttattacaa
      301 gttataaaaa acgcatttca ggaaatacta ccaaatagtg atattagagc tgatggacaa
      361 gttgttaaaa taaatttcca tgatggaatc aaatttgaaa tagttccagc ttttaatgaa
      421 aaagattatt ggggtgagtc aaaagggttt atttatccag attcaaacat gggagggaac
      481 tggaaagcta caaatcccaa aaaagagcaa gaagccatga aattaaagaa tacaaaaagt
      541 aataatttat tatatgctac gtgtaaacat tttcgacatg tacgagatac agaatttacg
      601 agttatcatc tttcaggaat agtaattgac agtttcgtat atgaggctat gggaaattgg
      661 aaattcgttg aaaataacag tgggggtcaa aacattagtt ctgtatctta tgagacagct
      721 ttattagaat actataactc acataaagtt atgggcggtt taaatttata ctctcctgga
      781 agcaatcaat ttgttaattc tgatagtagt atcatatgtt tagaaaaagt attaaaaaaa
      841 atagctttat aaagttttat ggaggaacaa catgggagac gaaaaaaagc gcttggaagt
      901 tcaattacgt gaagcatatg gacgagtaac gtatacttac accactcatt taaagcaagc
      961 cgataatatt atttctgaca ataaaatttt aaggtgtctt caaattggtt tgtcagccct
     1021 tactactgga ggggtactta gtaccattat tactgataat agtgagttaa cctttgttgc
     1081 tggattatgt tcaacaattt ctcttggatt aaacttatac tttaagaatt ttgatcttgc
     1141 tgaagaagct aaaaatcatc aaattgttgc cgataaatta tggttgatca gagaagaata
     1201 cgtaagctta ctaacagatt taaatcgttt aagtgttgaa gatattgtga aaaaaagaga
     1261 tcaattaatg aacgaaactc atgaagcata taaaaatgcc cctaaaacaa atagtaaaag
     1321 ctatgaatca gctcagaaag cattaaaatc tgaagaagag caatttttta cagaagagga
     1381 attggataag atgttaccag agcatcttag aaaaaagagt tctaaatatt gaactctttt
     1441 tttatgctta aaattctctt gtaatttttc tattcatcta tatttaagaa gaaatatgat
     1501 gatagaaaca tctcctaaag ccaccgcaag ccgttctaag catagcttag cccgacattg
     1561 ctagatatgc tcatctcacc atgaacgttt cctaaagcca ccgcaagccg tcctagccat
     1621 tgcctagtcc gacatcgcta gattagtgca tctcgccata aaagttttct aaagccaccg
     1681 caagccgttc taagcatagc ttagcccgac attgctagat atgctcatct caccatgaac
     1741 gtttcctaaa gccaccgcaa accgtcctag ccatcgccta gtccgatttt gcttgatcgg
     1801 ttccatttct gtttatttag tgggtcaaca ccgtaatctt aaatagccat cgcctattta
     1861 agattactag gtctgctttg aaggtaaaaa gtctttcatt cttcaagact tttaggcttt
     1921 caagctacgc ctgaaagcat tgaaattcat cgctctttat ttggttttgc catttataat
     1981 tccttacgcc cattatcttt ttcgcctgtc ataccttgtc agaagtgttt agagcttatc
     2041 tcatctttgg attaatacaa gtggcgtaaa tttttttcta aacgcaagcg ttacgaaaat
     2101 aatttactta aacacttgta ttaatccggc agataagaac gcattctaat cactcccgac
     2161 aaggcaagac aggcgaaaaa gataaataaa tgaacgaaag gaagaaacat aaaatgcaaa
     2221 accaaatagc gatgaagaaa aaaccacctc cctctaaaac aatccaaaca aaaaagaatt
     2281 ttgaaaaacc aaaaatcaaa aacagaaaga agcgttcaac atgagagata gaattttaga
     2341 agttaatcaa gaaatggtaa acaagttaac aaatatcagt gggaactatg aaaagtacga
     2401 tggtacttta cttgataact atatttttca cgatacacaa aatatcagaa ttggcagaag
     2461 taaaccgaga gcgttcattc tcatcaaaga gaaatatttg aatgaatgga atagcaccta
     2521 tgaaatggtt atgactgata actttgaaaa ggtattggat ttcagaaatg ctttgttggg
     2581 ttctgatatg ggaaatagca gtcttgaatc tattgaagtt gaaaacaaga ccgagaatga
     2641 aggtgaagga ttgattaaga atgtcttgaa aatcgaacaa aaatatatca gtgaagatag
     2701 aagtttttat agagtcacaa gctcggttgg tctagcaaac tttgctacta acttaattgg
     2761 aaacaatgct agtgaatctt tgctagtgat gtgtctgaat acaaagaatg aggtcattgc
     2821 ttatagcgaa gttttcaaag gtggaattaa tcaatcagta gcgataccaa gagagatttt
     2881 tcaactagct ttgctaaaca attcagcaaa aatcgcagta tcacataatc atccgagcgg
     2941 atatgaattt ccaagtgaaa atgacaaaag ttttactacg aatttgaaaa acgcaggaaa
     3001 aacaattggt attgaattgt tagaccatat catcgtaaca agtaaaaaca caagctatta
     3061 ctcatttcaa gaaaatggtt ctttataaaa attagagaaa agaggaaaac aagatgggcg
     3121 aaagaacaca agcgttaatt acagtcaaat atggtaacag caaaacgaaa gcaagcgtgt
     3181 cgattcatta tcagtggggg tatggtcgat atatgctgat ggacgtattg aatcaaggaa
     3241 tttatttaat caataaagca tacgacttac gagataaatc aagagaagaa ttagcaaaag
     3301 gaatctttga tagaagtaca ggagttatca attttgtcac ctatgaatct ggagtgagtg
     3361 cttatgatga aacagggttt ggtgggcgct atgaacctta tcaagaaacg attgaagatg
     3421 tgatgaagtt ttcagataac aatgatggct atgctcattt agagatggtg ttcgatgaaa
     3481 atggatttag tctagatgat aaaaccagat tgcgattgtt tgattttgag ggtcaacaga
     3541 taggactaaa caagttttgt gaaagcggaa aagagtttgc tacgaaagat tttattaagg
     3601 gatacagatt attgctagaa aattatggaa taaacctaga agaagagagc gtgaaataag
     3661 tgtttgaaaa aagaaaagac cagttcccta atttagaagc gaaaagtcaa aaagtgattg
     3721 atggtgaaat gattgaatta ttccaaaaaa gttatggaac catagaaact accgcaagtt
     3781 gcaaatacta ctttcaagga aagagaaaca cgtttaaagg ctttgccgat tctgatgaag
     3841 aattggcaaa gaaaatcatt gattggcttg tcagcagtga acacgtagca agagaagata
     3901 ccgttagaac aacgattaat ggggaagtcc taacaggtac tagatgtgtt tgttgtaatc
     3961 atgaatttgt agaagatgaa ggttgctacc gaatcaatga atatggtgat tttatttgcc
     4021 agagttgcca cgagatagaa tttccaaccg aaagaagttg gtttgaatac atgctacaag
     4081 attatgagat ggaaacagaa aatggtgaag tagttttttg taccaaagag gaattgagta
     4141 agagaagcga tgatgaaatt gaagattggt gtgtgtggtt agtagggaca gatgattgcg
     4201 ttgcatatta cacaacagca gaataggagt gtgaatcagc atggaaggaa tggattttat
     4261 agatcatgaa gatttgattg attttggtta tacatggaaa ggaatggtag gtattagcag
     4321 aagtcttgct aatgcctttt acgaaagaaa ttatgcagtc tatgtccttt atgatgacaa
     4381 tacagaaagc cttgttgatg aagagtataa attggattta gagaatgttt tatatgggat
     4441 agaaaaagaa gatttagcaa aatatatttt ttcatggttg ggtcaataaa cgaaagaggg
     4501 tagcaacatg acaaaattca aaagtaaagg tatgcaatgg tgttcttatt attatgttgg
     4561 gaatgatgaa gagcttttga gaaaagccaa acaaaaaggt tatccagata agatgttaaa
     4621 acagattggc tattacatgc cagaagagag aatagaaaaa gaatcttgta aggttctttt
     4681 tgatagtgaa cagctattag ttttgttaac tcaaaataaa ttgatttgtc aatttgaaaa
     4741 taattaatat ggggtgaaga accatgaatg agaataagat ggtattaagt gaatacgcaa
     4801 gattgagaac cattaattgg ttgaatggta tgcaaagtag tggcgctttt gatggatttg
     4861 atgaggagaa tatggacttt gaaagtttat cgaatgagga tattagatat tattttatcg
     4921 aatggtattt tacagaacca gatgaagagg tggaagatga accagaatta attttagaac
     4981 gtcaaagaat tttattcagt gaacaagata gtaagaggtt gtatggttta tatgacttac
     5041 aactgaaagg attaaaagcg cagtatgttg ctgatagtta cgatgatatg gtagaacagg
     5101 gggcagatag ggttgtcact tattcagaag atgatgaaga ggaatgggaa aactattcag
     5161 atgatgagat atgctttaat gatgggtata tgcctattga aatcaccgaa gaagaatatg
     5221 ataaagtgtc ggatagcgat gagattggta tgtgtacggt tgtaagtgga ttggatatag
     5281 atgtccaaag agtagaggtt gcataccaga agatgaaaga acgtataaaa agtaaagtag
     5341 aatcttgatt tgattggatt gtttgcaaag tagctaatta agagtataat ataatcataa
     5401 atgtagttaa tggatataat aatgagtata ttatactcaa aagaggtgaa cgaaatgaag
     5461 gtaaaaaatc caaaagaaaa aagcagaatt caagtcggaa tagacaagga tttaaaagaa
     5521 aacgcagaaa tgatattgga agagctaggg ttaaatccaa caacagcaat cacaatcctt
     5581 tataagcaag tggtcgctag aggtgagttt ccagtagaga taaagctaag cgaagaggaa
     5641 aagcaaggaa tcagattgca acagctaact atggatatgc cagttgatgt tctagataca
     5701 gatgaaaagt tagaagagtg gttcaatgaa gcctaatgag atagtaacgg tctatgttgc
     5761 atttacagat aaaaatggtg gtaaaagaag acctattttg gtggttagtg ataaagaaga
     5821 tagagtagaa ttttttggaa tcacaagtca atatgagaat aaatcagacc aaataaagag
     5881 agtgtatttt cctattgcag aatgggaaaa agtaggtcta aagaagcaat catggattga
     5941 cgtagggtct ttaaaggcta ttcccaaaag ccgagagaac atatcattta aaaaggttgg
     6001 tatgctatca gctattgata cgaaaaagct aaataggttt atcgagagat tacaaagaaa
     6061 agtctaacaa ggcttttctt tttttgttta tatgtatatt aagatatacg tttatataaa
     6121 cgtattt
//
LOCUS       DAZUKA010000153         5971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000153 DAZUKA010000000
VERSION     DAZUKA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.156"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..442
                     /locus_tag="ACG8PN_002510"
     CDS             <1..442
                     /locus_tag="ACG8PN_002510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4862633.1"
                     /translation="LVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            570..1253
                     /locus_tag="ACG8PN_002511"
     CDS             570..1253
                     /locus_tag="ACG8PN_002511"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4862634.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1562..1635
                     /locus_tag="ACG8PN_002512"
     tRNA            1562..1635
                     /locus_tag="ACG8PN_002512"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Arg,seq:tct)
     gene            1794..2153
                     /locus_tag="ACG8PN_002513"
     CDS             1794..2153
                     /locus_tag="ACG8PN_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4862635.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2276..3907
                     /locus_tag="ACG8PN_002514"
     CDS             2276..3907
                     /locus_tag="ACG8PN_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4862636.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3969..4610)
                     /locus_tag="ACG8PN_002515"
     CDS             complement(3969..4610)
                     /locus_tag="ACG8PN_002515"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4862637.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4796..4872
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4892..5200
                     /gene="rplU"
                     /locus_tag="ACG8PN_002516"
     CDS             4892..5200
                     /gene="rplU"
                     /locus_tag="ACG8PN_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4862638.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5227..5571
                     /locus_tag="ACG8PN_002517"
     CDS             5227..5571
                     /locus_tag="ACG8PN_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4862639.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5584..5877
                     /gene="rpmA"
                     /locus_tag="ACG8PN_002518"
     CDS             5584..5877
                     /gene="rpmA"
                     /locus_tag="ACG8PN_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4862640.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAEVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1851 a         1004 c         1286 g         1830 t
ORIGIN      
        1 cttggttccc cgaatttttg gtttgactcc ttatattatt gaggacaaag aaatgagtcc
       61 atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg agattttagt
      121 cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat
      181 agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga cttatattga
      241 gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga ttccatatat
      301 tggactagtc ctttcaaaaa cggcatttgc atggattcag gggttattct ttacgattgc
      361 tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa agaagcggag
      421 acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag agatccaacg
      481 actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt
      541 tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat cgtcctgtag
      601 tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg ggtgagctag
      661 gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat gaaacagttg
      721 tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag atcggagcag
      781 ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct catgttacga
      841 caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa cgtctaggac
      901 tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg gagttgtctg
      961 gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa tttttacctg
     1021 aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc ttccaaggag
     1081 gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc catcgtaaat
     1141 taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca atcttgccat
     1201 taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg tgatacatga
     1261 ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt gatgtaaaag
     1321 tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag gagtagcatg
     1381 aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg agaatgtctc
     1441 gttttaaaag attgatttag tagttgggcc aaaataattc attatttgct aattaaataa
     1501 ctatttcgta ttgacagaag caatttaatt atctataatt attgatgttc cgctgattct
     1561 tgtcatagta gctcagctgg atagagcatc cgccttctaa gcggacggtc gggggttcga
     1621 atccctcctg tgacgctaaa aaaccgttag aaactgaggt ttctaacggt ttttctttgt
     1681 tttttgtgcc aatattgttt gttttcaaag gcaaataaaa taaaaaaaca tccttatcga
     1741 gtacaattat aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa gatatgacga
     1801 tactatctag aattttagtg actctagttg caattgaatt tttttatatc atgtatatcg
     1861 aaacggtgag aactgattct gacacgacaa gtagggtttt taaaatgagt aaagaagagt
     1921 taagcagaaa atctgttcaa acattgttta agaaccaagg agtgtacaat ggactattag
     1981 gagtgggact actttatgga gcttatcttt cttccgcatc taaagaaatc acaagtatgc
     2041 tattaatatc tatttttttt gttgcattat atggcagttt gactagcgat cgttggattt
     2101 tactcaaaca aggtgggcta gctctgttgg cattgctttc tattttctta taaatataga
     2161 aaaaataaga atttatctaa caggaaactt tgtgaaaaat attactttat ctcatgaaat
     2221 atgatattct aatgaatggt attttagaaa atttcatgaa aaaggagtta gataggtgtt
     2281 caaaaattat gtttcctcac taaaaaaaga attttcaggt tatgatcttt caagatttca
     2341 gaaagatctt ttggctggga tcacagtagc tgctgtagca ttgcctttag ccttggcttt
     2401 tggggtatca agcggagctg atgcggccgc aggtttgatt acggctatca ttgccggtat
     2461 cgttattggc ggtttatcag gcggttttta tcaaatatca ggtccgacag gtgcgatggc
     2521 agctatcttg atgtctattg cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt
     2581 tttagcgggt ctatttttac ttgcagcagg acttttccga ttaggaacac tgacttcttt
     2641 tatcccagca cccgtaatta cggggtttac ttcaggtatt gctatcatta ttgcattagg
     2701 tcagatcgat aatttatttg gtgttcactc ggaaggtaca aacgttattg aaaaactaag
     2761 tagttatcaa acgataggat ttccgataaa cacagcctct ttgatcatgg gagggctagt
     2821 cattttaggg atgtttatct atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct
     2881 ggctatcatt ttggcaacag cagttatgct attgttccat ttaccagtag caactgtggg
     2941 taagattcca caaacattga tcagtagtaa tcgtttgaat atgggagatt ttagcttttc
     3001 tgccttgcaa caagtggcag ttccagctat tagcattgct ttattaggaa tgattgaaag
     3061 cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag cccttagata gcaatcaaga
     3121 attaatggcg caaggaattg gaaatctgtt gcttcctttc tttggcggga ttcctgctac
     3181 tgcggccatc gcccgtacaa gcgtggccat caaatctggt gcacaaacta gattagctgg
     3241 tatgatacat gcagttgtct tactgctctc catgcttgtt tttgcgccag tgatgtcaca
     3301 gtttccaatg ccagcacttg caggtgtttt gattgtgacg gcttggcgta tgaatgaatg
     3361 ggaaacgatc aaggagctat ttactaataa atattggtca gcagttctat tgtttatcct
     3421 tacgatgatt tgtacagtta tctttgattt aagtattgct atcgtgatcg gggtgatcgg
     3481 cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg atttcattag aaacgatcga
     3541 ttggcaaaga atggggctgc cagaaacgga aaagctgaat aactggacag tagtgtatat
     3601 tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa aataaattgg aagaactaaa
     3661 tcatcacgat ggtgtcattt tctccatgcg aggcgtacca agtattgatt tgacggcacg
     3721 tgcagttttt gatgaatttc aagaaaaagc agagcaaaga ggacaaacga ttatctatac
     3781 ttccttgcag ccagaagtag aaaaacaact ttctcacttg tgggaacata cattcacgca
     3841 tcaacatcaa acagttgctc atgcgttgac agctttacat gcgaaatata ctgcaattag
     3901 ccattaaaac aatgaaaaag aacagacaag actgtaaact gaagatttta tggtcttgtc
     3961 tgttcttttt aatattgctg taaaagtgaa gctagtactt gccagtcttg atggataaca
     4021 ggtacaaggg agcgattgta gaaaatagct gctggatgaa aaagtggaag aattcgatag
     4081 tttttttctg accattcgta ggcatccttt tgttctgtta attgtaagac aggccctgta
     4141 tagagttgac catgattttt ggtaaccgta tattctttgc caagtaaacg ttgaagacca
     4201 atgtttccaa gtgtcgcaat taattttggc tggaccttca tcagttcgta atccaatatt
     4261 ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat ttggataaac agtttctgac
     4321 tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg ggcggctcct tacagcactg
     4381 gtgatataca cctcttctcg tgttaagcca gttgaagaaa gtgcctgcat caattctttt
     4441 ccagcttgac cgctaaaagg aacatgcgta tcaatctctg ttttccctgg ggcttctccc
     4501 acgagcatca gtttaggatg aagaggtcct tgcccagcga taaatccttc tacaggacgg
     4561 ccgttcattc ttaaagaagc ttcttgtatc agttcttttg ggtattccat ttatgctcct
     4621 cctttttctt ttattataag aataaaagaa acacaatgcg actattttat ttggtagaac
     4681 tgtgaggata gaaaagcaga agtattgctt gaatttttgt tttacttttt gattttaaat
     4741 ttagttgact aacggttgct gttctggtaa gctagtactg ttgtaattgt ggactccaca
     4801 gctacaaccg ctcagaacaa gtaataagca cagcatatgc tgtcacttcg gatggcgagt
     4861 ctaagtatat cagaaggagg tgctttatag catgtacgca attattaaaa ctggtggtaa
     4921 acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg
     4981 agaaaaagtt gtttttgacg aagttatctt agtaggcgga gaatctacaa aagtaggcgc
     5041 tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa aaacacggca aacaaaagaa
     5101 agtcgttact ttcaaataca aacctaaaaa acattcacac cgtaaacaag gtcatcgtca
     5161 accatataca aaagtggtta tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc
     5221 ctatatatga tcaaaggaac atttaagcga aatgacgcag gtcagatcgt ttcattcact
     5281 ttgaccgggc atgctgatgc tggtccttat ggaagtgata tcgtttgtgc gggtgtttct
     5341 gcgttagcga tcagcactgt caacgggatt gcctcattag ccggttttga gccgattgtt
     5401 gaaatgaatg aagaagaagg cggttatctt tataccgaag tcacttcagg aatgacacaa
     5461 gaacaaaaca atattgctga gattctatta gaaaatcttt tattaggatt acaatcgata
     5521 gaagctgaaa actcagaata tatccaaatt aaaacaatta atgataaata ggaggtgcag
     5581 accatgttgt tatctatgaa tctacaatta tttgcccaca aaaaaggtgg cggttctaca
     5641 tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca
     5701 gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac
     5761 gtgggtatcg gtggagacga cactttattt gcagaagttg atggcgtagt acgtttcgaa
     5821 cgtaaaggcc gtaacaaaaa acaagtgtct gtttatccag tagctcaaga agcttaattg
     5881 aatcttatga ttaaagaggt tgtgacaaga cttttgtcac agcctttttt gtttataaaa
     5941 ataaaagttg tagtggaggc tacgaagttt a
//
LOCUS       DAZUKA010000154         5887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000154 DAZUKA010000000
VERSION     DAZUKA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.157"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..571)
                     /locus_tag="ACG8PN_002519"
     CDS             complement(113..571)
                     /locus_tag="ACG8PN_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4862641.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(584..1915)
                     /locus_tag="ACG8PN_002520"
     CDS             complement(584..1915)
                     /locus_tag="ACG8PN_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4862642.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1926..3368)
                     /locus_tag="ACG8PN_002521"
     CDS             complement(1926..3368)
                     /locus_tag="ACG8PN_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4862643.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3355..4119)
                     /locus_tag="ACG8PN_002522"
     CDS             complement(3355..4119)
                     /locus_tag="ACG8PN_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4862644.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4223..5098)
                     /locus_tag="ACG8PN_002523"
     CDS             complement(4223..5098)
                     /locus_tag="ACG8PN_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4862645.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5353..5437)
                     /locus_tag="ACG8PN_002524"
     tRNA            complement(5353..5437)
                     /locus_tag="ACG8PN_002524"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5401..5403),aa:Leu,seq:caa)
     gene            complement(5455..5526)
                     /locus_tag="ACG8PN_002525"
     tRNA            complement(5455..5526)
                     /locus_tag="ACG8PN_002525"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5492..5494),aa:Gln,seq:ttg)
     gene            complement(5536..5608)
                     /locus_tag="ACG8PN_002526"
     tRNA            complement(5536..5608)
                     /locus_tag="ACG8PN_002526"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5573..5575),aa:His,seq:gtg)
     gene            complement(5670..5754)
                     /locus_tag="ACG8PN_002527"
     tRNA            complement(5670..5754)
                     /locus_tag="ACG8PN_002527"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5718..5720),aa:Leu,seq:caa)
     gene            complement(5775..5845)
                     /locus_tag="ACG8PN_002528"
     tRNA            complement(5775..5845)
                     /locus_tag="ACG8PN_002528"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5811..5813),aa:Cys,seq:gca)
BASE COUNT         1799 a         1253 c          896 g         1939 t
ORIGIN      
        1 ataattaaca aaaatcaaaa tgattaaatc tagtgattga aacatatcat ttcaaaaaaa
       61 ttttgtaaat tcacccataa atatcaccct atttaacttt tttcatcgta cactaaaatt
      121 caaaacaact atctttaatc tttacttctt ggttttgttt taataaattt ccttcaaaaa
      181 tgcctggaga aactacaatg atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat
      241 ctataatagc taacagtgtc ccttctttta cctcctggtt actttcaacc cgtatctgaa
      301 acggacttcc ttcgagttca actgtatcta aacccatatg gatcagtatt tttctgccag
      361 attcactttc gatcgttatg gcatgtcggg taggaaatat attatcgatt attcctttaa
      421 ctggcgcatg gatatatcca tcatgattcg ttatagcgta tccaactcct atcatttttg
      481 aagaaaagac ctcgtctttt actgctgtta atggaataat ttgaccacta gctacagctt
      541 ttagaatgtt tcttgtttta aagaaatgca ctttttatca cctctacttt aatgctggcc
      601 aatatttttt gtttactttg atcatttcat ctagtatttt ttttgcgact ctggcatttg
      661 gtacggtttg attcaatgta aatgcttgta aagctttttg ataagaacct tcaaagtaag
      721 catctactag taacttctct actgcaactt gtgcctccat caatccttta tgaaaatcac
      781 tgattggttt tcttagtgta attggctcca caccagttgc accaacatat gccggtattt
      841 caacaactgc atctgaccgt agattcggaa tcgccccttg attaggaacg attagcataa
      901 agcgacgacg atcatcattc aacaaggaaa tagctatatc gacgatatat tgtccatgct
      961 ccccaaaatt aaagttcaat acttcttcca caataccgcg gcgtatttta tcagccattt
     1021 ctttggtatt tttctctcgt ccatccatga ccatattagc acgtgtatac tctttgtcag
     1081 tatgctcaac gaccatatca ggatataagt aatactctaa gtaattattc gggatattac
     1141 ttggaaaatt tttcgtgatg actgacatca tttgaaaggt tttttgccaa gttttatctc
     1201 ctatattaaa atcggcgact tgcatttctt ttgttattag tttttcaata atctctggca
     1261 taatttctcg ttgcaattct ttatcataaa tcgatgtata ccagccaaag tggtttaaac
     1321 cataatacgt aacgatccaa ttcttacgat cataaccgta attcactgca atcgtttctt
     1381 caattgaaat agtcatatca caggcattaa tcatttttac attcggatat ttacgacgaa
     1441 cagcttcaga aacgatcgac tctggatttg tataattcaa aatccatgct ttgggggcat
     1501 agtcttgtat atgatcgaca atttccaata aacctttgat tgagcgcatc ccataagcaa
     1561 atcctccgag tccacacgtt tcttgtccta ctaatccatg tttaagtgga atcttttcgt
     1621 ccttttctcg catctccaat cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg
     1681 taaaagcaag ctttggatca tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt
     1741 tcagcataaa tttaacaatc aagtacatat ccatatttct ttcttcatca atatcatata
     1801 aacgtatttc agataatggc aaccgttttt cattgttcaa aatggcttga acgattcctg
     1861 gtgtatacgt actcccgcct ccagctatag tgataatctg ttttttcatc atcatctttt
     1921 cctttctatt cttctaataa tttattgaat tctgccataa cataagtcac ttctaagccg
     1981 atgataattt gataattttt ttcagttggt ttaatgatcc cgcttatccc taattgcttg
     2041 attcgctgtt cctcgaccat ggtgggatcc actacctcta cccgtaggcg tgtattacaa
     2101 ctatagacat ccaggatatt ggcttttcct ccaaacgctt gtagcatttt tgttgccata
     2161 tattcataat ttttacttgc taaccctaac tctgtttctt ctaaggttac ttcattgcca
     2221 aactctgcct tagcttcacg tcctaatgtc tgaatgtttc tcttattgat caaataataa
     2281 aaagtaacgt aatacaaggc aaaaaagaaa attccgattg gaagaatcaa aatagcattg
     2341 gtagcgattc gaaaattgat gagataatca acaatacatg ctccgaatgt aaaaccaatt
     2401 cgtatattga ataagtaaca gacaatccct gctaaaccgg caaaaagtgc atggatcacg
     2461 tacaacatag gagaaagaaa catgaaacta aattcaacag gctcagtgat atttgcaata
     2521 aatgaagttc ctgcaccgct tcccatgatt cctttgacca tctctttatt ttctttataa
     2581 gctgctttat agatagctag acatatagct ggtataccaa acatcattac tacaaaaaaa
     2641 actgataaaa acagtccagc ctgaggatct ccgttgataa agcgtgtcat ctctcctctg
     2701 aaaacttcct tgctaggaga tgtatagctt cctaaatcgt aataaatata cgtattcaac
     2761 acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa acataaatat ccccacacct
     2821 aatgctccca tatcaacgag cagttttgca aaattatcaa tcccaatttg gatgagcggc
     2881 cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat agtcaatggg
     2941 aatttttcgc ccgcaaaata agaaaaaact actggtaatt tttgttcttt aaagcgatta
     3001 tacgtccatg ccgctactaa accggaaata attccaccaa aaatccccat attcacttct
     3061 gggtccataa tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca
     3121 ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc aaataagata
     3181 tccaagtttc caaaaacggc atttccagct gcaccaatta ctgggatgtt cagcatatcg
     3241 tcagctgata aacgcatgat caatcccgct aatggcatcg ctgcaactgg aagtagcatc
     3301 gctcttccaa gcttctgcac atttgattta acattcattt ttattcctcc tctattaaat
     3361 atcctcatat gttactaact caatcaaagg atcatttttc actttttgag caaatgctga
     3421 atgaattaaa aattcgactt ggtgggtacg aggatacaca aaacttgaac catttaacaa
     3481 ttcctgatcc aaatacgctg cgtgacacat gacttcgatc gttttatctt ctctcaatat
     3541 catgagtaaa cttgccaaat attcttccac actcgtattg atttgtcgtt ttttttcatc
     3601 agtttctcct acgacatcga aacaaggctc aaaataatcg acatgacgaa cttcatcttt
     3661 tcgttcaaag ttgctccgaa ccggcaaatc atattttttt gctaatgtaa caactacctc
     3721 ctgattttca tccaaagtat gagtatgatg atgactgtct aaatgagttg gtatgatccc
     3781 cgcatgataa attttttgaa tctgagcatt ccattcctga tacagttgat ctttgtcaat
     3841 gttaaatgga tgctgataat aagataagga tctgaactct ccgctttcga taagagtatc
     3901 gacagttttg aggagtggtt tgccacacgt caatgttaaa tggactccta cacctagcct
     3961 aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt
     4021 agttgatgtc aaaattcctc gctgataagc atccataatc ccataattca caccatggct
     4081 atagccaaaa tcatccgaat taaaaatcac ttttcccatt tcattctcct tcttttttct
     4141 actaaaatag gcgcaaccaa ataatcatag tccatttact atgtgtcatt tggttgcgcc
     4201 tatagatagt tacgccctta ttttaataga ttcttgctag atgaattgcc aaatagatga
     4261 tttcattttc ttgaatggca agttgaaatt gctgttcgat gatatcttta atctttagtg
     4321 ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata ttttctactg
     4381 gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt gatatgaacc attaagcgat
     4441 gataagtcag tgattcttta tcataaacgt ttgaatagga tttttctaga tgggacaaaa
     4501 tcgtggtcgt catttctact atttttaagc tctttaaact attaggctct cctagctcag
     4561 cattaatgat atgcatagtg atgaatccta cttcatttga ccctaactcg gtttgatatc
     4621 gatgattgat tcgctcgacc acttctactg ctatatcata ttcatttgga taaaggaatc
     4681 tcatttctgg caacataatt gctgtgacat ctctatcttc tctttttcgt tgaatagcaa
     4741 aataaatatg atctgtcaat gtaatataga tatttgaatc taatggagag gagatcctag
     4801 ctgttatcag ttcaatcact tcttccgcaa aatccataaa ttcgattgga atctgcgaga
     4861 ctatctcact gaatcgtttg atcttttctt tagaatctaa tacaaataat ttgtcagcat
     4921 cctcaacatc aaatggctta ctcactttag cagaaaagcc aatccctttc cccatcgcaa
     4981 ttttttctat tccatcgtca tcgatcaata cagcattatg attcagcttt ttcatcacat
     5041 acaccgttaa ttcacctcct ctatatgata aaacaaaaca acttgcagac tccttcatac
     5101 atatttttgt ctccaaacaa tttttaaaga caaaaaacga cctaagaaag aaccctttct
     5161 taggtcgtgc ctaattgaat agtagcaccc tacataattt acaaaaatag tttaacacat
     5221 aataaaagcg ttatcaatat cttttttact cgttttatga taatacttgt atcgaattga
     5281 tttggaggta attacataaa aacaaaaaaa atatagcaac tctctataag aaagttgcta
     5341 tattgataat tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt
     5401 ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata ataactgggg
     5461 tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat
     5521 accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc
     5581 ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa tttctcaata
     5641 aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac cggcacgccc
     5701 tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaagttg
     5761 atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt tgcagacctc
     5821 tgccttacca cttggctata ccgccattat actattatca atctataaaa taaagaataa
     5881 attatag
//
LOCUS       DAZUKA010000155         5847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000155 DAZUKA010000000
VERSION     DAZUKA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.159"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..994
                     /locus_tag="ACG8PN_002529"
     CDS             209..994
                     /locus_tag="ACG8PN_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4862646.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1007..1858
                     /locus_tag="ACG8PN_002530"
     CDS             1007..1858
                     /locus_tag="ACG8PN_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4862647.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1982..2434)
                     /locus_tag="ACG8PN_002531"
     CDS             complement(1982..2434)
                     /locus_tag="ACG8PN_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862648.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(2899..3267)
                     /locus_tag="ACG8PN_002532"
     CDS             complement(2899..3267)
                     /locus_tag="ACG8PN_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4862649.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3417..3899
                     /locus_tag="ACG8PN_002533"
     CDS             3417..3899
                     /locus_tag="ACG8PN_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4862650.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            3936..4946
                     /locus_tag="ACG8PN_002534"
     CDS             3936..4946
                     /locus_tag="ACG8PN_002534"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4862651.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5061..5774)
                     /locus_tag="ACG8PN_002535"
     CDS             complement(5061..5774)
                     /locus_tag="ACG8PN_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862652.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1920 a          958 c         1167 g         1802 t
ORIGIN      
        1 ttgtaaatta cttcttattt cttgaagctg gccacttctg tcacaacctc tttctttata
       61 gaaataaaag gacactagca attcatgaaa acgattgcta aagcgtatca tttgctttag
      121 actatatatc ttggtgtaca ctcactttaa gacggaaata aaagttatcc gtaataacag
      181 gtttaggtat gaaaaaggag tgggtactat gtataaggaa ttagaaaaca aagtcgcagt
      241 agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg ggaaagaaaa
      301 aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag cagtcaaagc
      361 aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt ctgaagaagg
      421 aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg tttggattaa
      481 taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag attgggaaag
      541 agtattaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt taaattactt
      601 tacagagaat gataaaaaag gaaatatcat caatatgtct tctgttcatg aacaaatccc
      661 atggccaacg tttgctcatt acgctgcttc aaaaggcgga gtaaaactat tcaccgaaac
      721 ggtagcaatg gagtacgcac caaagggaat cagaatcaat agtataggac ctggtgctat
      781 ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac tcttggaaag
      841 tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag ctgcatggtt
      901 ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg gtgggatgac
      961 tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg gtttattgat
     1021 tgcattgatc ccggcagttg ggtgggggat ccagccactg attttgaaaa agattggtgg
     1081 cagaccaaca aatgagatat taggaactgg tattggagcc gtgctcgttg gcttacttgt
     1141 tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta gtttactttc
     1201 tggtgcattc tgggtcatcg gacaaacagg acagtataca tctttcaact tgattggtgt
     1261 ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcat tgattggtgt
     1321 cttcgctttt ggagaatggt ctggaagtat gggaaaaatc attggtgcag cagcaatcat
     1381 tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa aaaaaggcgg
     1441 tatcacaaaa ggaatcagtc tattagcatc aacaagtatt ggttattggg tttacagtgc
     1501 attgcccaaa ctagttgatg caagcggact ttccattttc tttccgcaaa tgatcggtgt
     1561 ctttttaggg gcagtgattt atgtgatttt ccgaaaacca accgcatttt ctgaagacaa
     1621 gagttggaaa gcaacaatag ttggagccat cttcagtgtg tcagcattgg cttatatttt
     1681 ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa acgtagttat
     1741 ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag aaaaaaaatt
     1801 aacgattatc ggattattgc tgatcgtagt aggaagcatg gtcacggtct ttatttgagg
     1861 ataaagatgg taaaatctca tttattaaaa aaatatttgt ttgaaccaaa cagtaagtac
     1921 ttataaaaaa atagcgctag aatttccaac atctctggaa tttctagcgc tttttaattg
     1981 tttaagatac aagtgaaata tctgtctgca tcaataattt ttcattgatc cattgactaa
     2041 aatttacgac atgattgaag ttaccaccga ttagtatctc cgcatgattg tctttataaa
     2101 gatcgtattg taaaagacgg tcttttttat ttgcgtaaac atcgttatgg tactggacta
     2161 attgttctcg gatttgattg attgctgtaa aatcacgata ctgaaggttg aaacgcatcg
     2221 tgattggtgc atttagcatg aatcgctgca tttcttttgc tgcttcctct gcttgatctc
     2281 cgtataggaa ctcaaaaaga ttacgttcag aaaatttcca ttgcccgcca acctttgttc
     2341 cttttagttt tccttcatga agataatttc tgatagaacg tgtagtaaca cctaattgtt
     2401 cggctaattc atttactgag taaaaatctt tcaatgttca ccctcctatg tgaatggttc
     2461 tactgactta gttgatattt gcttaatcac gaaatgtaga aagaacgatt ttaattaagg
     2521 tcattgtaaa aatcaagtca gaaaattcta acttttcttt ctctaaagaa attcgttctc
     2581 tcctaaagtg atattatatt taaatatacc tgaaaataat gggcagtatt atgaaagatc
     2641 actgatattt tctcgtattt tttccttatt caaaagtttt gcaggaaaat tgctgaaaaa
     2701 ataattttac taagtggaaa attcaaagcc attcaatcag tgattttttt cagcacaaga
     2761 aagactcctt ataaaaatag cagatacttt gtcaattaag tgtttaacca gggatgataa
     2821 aaaggatagg attgaacaaa acctgtataa gctgatgaat agtttcctcg ccagtaaaag
     2881 gtttattcgt ataaagcttt atcgccaaaa tttatcatga ccttttttat cttcaatatt
     2941 aaatgaaatc aattgttcaa tcaattccca gttgattaca cttttccaag gaactttgat
     3001 gacgttatcc gtatatacgt agcctgcttc ttggatattt ttttcaaggg cacgaatcgc
     3061 tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa ctaaatgcaa taataaaggt
     3121 accatggtca gtaaacatag gttggttcca ttttacagtt gtgtctagct ggggatacgt
     3181 ttctgttatc ttagcaaaaa ggtctttgat cttttgttga tgttcttttt ctttgatctg
     3241 attgatgatc ggctgaaatt tttccattct aaaacgcctc cattaagtgg tttgatttat
     3301 tttaccagaa aaacaatagt tatgtataaa aaacgaacaa aacacaagta atgttgtaac
     3361 cgctatattt atatgataag atgtctcaaa taataaaata gaaaagagac ataaaaatga
     3421 ttgacttggc aaagaaattc ttacacgaaa atatatattt tgagttgtat catcatcgag
     3481 ccatttatac aaatgaagat gctttggtaa taaagaaaga acaaggattt acaggtacag
     3541 agaccaaaag cttattctta aaagataaaa aagataataa ctacgtatat ctgactttta
     3601 ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg ctttctattg
     3661 tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt tctccgtttg
     3721 gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat gaaaaacttg
     3781 tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac ttaaacaaaa
     3841 tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa aacaattaac
     3901 caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca ttattagaaa
     3961 gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa caccattcgt
     4021 tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt actggaaaga
     4081 cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca gatgaattca
     4141 atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gccttgggga ggagaattat
     4201 tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg atagtaggtt
     4261 attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata gccactgcac
     4321 atggaaccaa ccttattgat ttaagaggag agtacttgga tgaaacgaca gcaatgtggg
     4381 aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaaccttct gaaaagtatc
     4441 aaaaagagtg gccccatgag cattcatcat cttctgtttt ccatctcact gaaccaacaa
     4501 attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta ggtggctgta
     4561 ttgatacgat cagtcattta atcggtaccc cttatggaga tgtccaatct tttaaaagaa
     4621 agtataatga tggaaagccg atcttgtggt ttctagaaaa ttgtgagcta tcgtcagttg
     4681 atttaagaag atcacttgtt cagatgaaac tggcaggatg gtttgatggc agctcgggga
     4741 tcatgttcgg tagaagtgca gcagaggatt tgttagatta taaaatgaag gatgtatatg
     4801 aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt ggtcatgtac
     4861 cgccacaact gacattgatc aatggtgcct atgcagaagt agcggtagaa gatagaaaag
     4921 cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtt tatcaaagac
     4981 caacaaatct aaatagaaaa gaaaatgaaa gaagctgaca gtattttgcc agcttctttt
     5041 tgtatgatct attagaaatt ttaattatcc ataactatca cattgatcag ctgtttcaca
     5101 tcatttggat tacctgtagg aatagctact acagatgcat tatcaataat ttcttgagtt
     5161 gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca atgtgaagga
     5221 actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac tccctggtaa
     5281 ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc tgcatcataa
     5341 ggggcgatag gtgttcctgc aggataatga ataacatgta gttcgaaatc acccgatgta
     5401 tctctaaaag tattacctct aattgctagc caaacacgtg cgtactcaat ttgtagatca
     5461 gaatatcctg ctaacggatc tattgactga gaagactgtg aggaagttgt gtctattgtt
     5521 gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt gggctctgag
     5581 actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg agtacttgat
     5641 ttttcagctg aactggtttg tttgattgaa cttttgtctg tggtcgcaga tggatcacta
     5701 ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa taaaataaaa
     5761 cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag cataatcctg
     5821 aatttttgaa tccgtaaaac cttgtct
//
LOCUS       DAZUKA010000156         5801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000156 DAZUKA010000000
VERSION     DAZUKA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.160"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..993
                     /locus_tag="ACG8PN_002536"
     CDS             97..993
                     /locus_tag="ACG8PN_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4862653.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1143..2084)
                     /locus_tag="ACG8PN_002537"
     CDS             complement(1143..2084)
                     /locus_tag="ACG8PN_002537"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4862654.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2210..3331
                     /locus_tag="ACG8PN_002538"
     CDS             2210..3331
                     /locus_tag="ACG8PN_002538"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4862655.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3589..5091
                     /gene="eat(A)"
                     /locus_tag="ACG8PN_002539"
     CDS             3589..5091
                     /gene="eat(A)"
                     /locus_tag="ACG8PN_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4862656.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5158..5685
                     /locus_tag="ACG8PN_002540"
     CDS             5158..5685
                     /locus_tag="ACG8PN_002540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4862657.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1901 a         1027 c         1221 g         1652 t
ORIGIN      
        1 aaattttcat acaaatatag gaaacgtttt actggctttt tactatttaa acaggctatc
       61 ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga acaaaaatga cgtactgcta
      121 gttgcaaaaa ataaatcact ttattttttg ccttccatcc gctctttaga tgtttggtcc
      181 ggaaaagatt tactacagtc ggaattcatt aaatcagcta gacaacagtt ggcaaattat
      241 cagctgatcg tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgccca
      301 tttaccaatg caaaaattgt gttatttttt tggaatcact tcaaagcaga acacgaggcg
      361 atgctgaagc aggcacaaga tgaaccggca atcgatgaga tttatcattt tgattttttg
      421 gaagcaaaag aacttgggct gaaacataat agttcatttt attcgaaaca tatgaatttt
      481 tcttctagcg aaccaaagac ggatctattt tttggtgcaa cgaataatgg gagaaaagaa
      541 cgagcagaaa gctataagca ggaatttata aaaagagatg ttacgaccaa ctatttcatt
      601 cttccacatc gagggaatga acaacctgga tatcttactt atgaagagta cctgaaaaaa
      661 acaggggaat cgcgcgggat tctggaactg ttgcgggagg gtcagcaagg agtgactttg
      721 cggacatttg aatccatgta ttttcaaaag aaactagtga cggataatca agcgattgct
      781 cattatcatt tttatcgtcc tgaaaatatt tttttgcttc gagaacgtga tttagatgaa
      841 ttaccggaat ttctgcatac accatttaag ccaatcctat cagagctatt ggaattttat
      901 gatgcagaaa attgggcaaa acgatttatc actaacgatt ccactattta tgagcaatac
      961 gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct acattttgaa gagattatgt
     1021 actgaaacaa acaagcacaa ttcttatggc atcatgagag ttgtgttttc tttttgatct
     1081 tttattgggc ttaaaaaaag agaaccagct gctgattcag ctgattctca tcagagagcc
     1141 ttttattctg cgccaaggat tccttctttt gcatggacta attgggtcaa aaaggcacaa
     1201 tatggtgtag caatatcata tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg
     1261 atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta atgcaacccg
     1321 atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg atctagtatg
     1381 attccttctt ttgctgcgat agaagcaaat tcagatacga ttgttcgaac catgctctct
     1441 gccgcttttt gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca taaaccattc
     1501 aatgttccat ttacacaagc ttttcgccaa atggaataac gaacgttatg gctatagatc
     1561 ggatttaaat ttgcttcttg aaagacttct acaacttttt ttgtaaatgc ttcgccttct
     1621 ggttccaagt tctctagttc gatttctcca tcacctaaca atgtgacttt tccgggacca
     1681 gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg cacatatttt
     1741 tccagtacat cttcatgacc taatccattt agtaaacaca aaacataagt attgtcagta
     1801 ataatggatt ggatcgaaca aaacatatca tctaattgat tggcttttgt taatgctacg
     1861 atcaagtcca cgtgttcgtt gctttcgatg atttcttctg gtgaatagat tggcagtttt
     1921 gcgaccactt ctttaccgtt aaagtcagca atcaacccat ttgtacgaat ggcttcaatg
     1981 tgagcaggcc aacgatcgat caagaggacc tcatttccgc tttgatgtag catcaatccg
     2041 attcggctgc ccattgctcc ggccccagct attgcgattt tcatgttttt tctctccttt
     2101 attcaatgag ttccctttta gtatatagaa aaatggaaga aaatagcatt gttttgtttg
     2161 aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga gggatacaga tggatatttt
     2221 ggcggcaatt gattcattta aaggaagtgc ttcttcattt gaactaaatc aggcagttct
     2281 aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag atggcggaga
     2341 aaatactgca gaagcaatct attacgcgct caggggaaca tggcgtacca ttgatgggat
     2401 ggatctctta ttccgtcagc gaaaaatcag ctatttattg acaacttggc aaggaaaaaa
     2461 caccgcagtc atcgaatctg cacaagcatt ggggttagat ctacttgata aaccaacgga
     2521 tcaaactatc cgacttgctt ccagttatgc cttaggagaa cttattaaag atgcactgct
     2581 tcaagaagta gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg atggtggtct
     2641 aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa atcctttatt
     2701 atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag taaaattgat
     2761 tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt ggcaatttgg
     2821 aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag caggaaagat
     2881 tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag gaagtggagc
     2941 agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt ctggtttcga
     3001 tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg tcatcattac
     3061 aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg gtgtagcagt
     3121 gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag accaagagct
     3181 agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc caataggttt
     3241 agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag gacagacact
     3301 tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt tatgctatcg
     3361 taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag tcgattgatg
     3421 aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga ttttcgggat
     3481 tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg catcctcttt
     3541 agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat gtctaaaatc
     3601 gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt tgaacaagca
     3661 aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag
     3721 acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag
     3781 caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg
     3841 ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa
     3901 acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa
     3961 gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg
     4021 aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa
     4081 ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta
     4141 gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag
     4201 aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt
     4261 aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa
     4321 acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg
     4381 ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc
     4441 cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg
     4501 aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat
     4561 gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt
     4621 tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac
     4681 ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa
     4741 aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac
     4801 caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag
     4861 atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag
     4921 ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa
     4981 caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa
     5041 accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa
     5101 tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg
     5161 ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg
     5221 gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta
     5281 gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat
     5341 acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta
     5401 gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca
     5461 cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg
     5521 atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc
     5581 cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc
     5641 tttacattca tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt
     5701 atttaaataa aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga
     5761 caattctttt gataatataa actcaacctt tagaaatgaa a
//
LOCUS       DAZUKA010000157         5748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000157 DAZUKA010000000
VERSION     DAZUKA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.161"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8PN_002541"
     CDS             <1..1152
                     /locus_tag="ACG8PN_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFS4862658.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8PN_002542"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8PN_002542"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4862659.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8PN_002543"
     CDS             2728..3003
                     /locus_tag="ACG8PN_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4862660.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8PN_002544"
     CDS             3009..3776
                     /locus_tag="ACG8PN_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4862661.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..>5748
                     /gene="typA"
                     /locus_tag="ACG8PN_002545"
     CDS             3944..>5748
                     /gene="typA"
                     /locus_tag="ACG8PN_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4862662.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAR"
BASE COUNT         1949 a         1027 c         1293 g         1479 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtga
//
LOCUS       DAZUKA010000158         5727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000158 DAZUKA010000000
VERSION     DAZUKA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.162"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8PN_002546"
     CDS             196..921
                     /locus_tag="ACG8PN_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862663.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8PN_002547"
     CDS             911..1420
                     /locus_tag="ACG8PN_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862664.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8PN_002548"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8PN_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4862665.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8PN_002549"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8PN_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4862666.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8PN_002550"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8PN_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4862667.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8PN_002551"
     CDS             4129..4902
                     /locus_tag="ACG8PN_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4862668.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8PN_002552"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8PN_002552"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZUKA010000159         5719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000159 DAZUKA010000000
VERSION     DAZUKA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.163"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..507
                     /locus_tag="ACG8PN_002553"
     CDS             <1..507
                     /locus_tag="ACG8PN_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFS4862669.1"
                     /translation="YKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIE
                     KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRL
                     ESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQ
                     AFERILTI"
     gene            788..2008
                     /locus_tag="ACG8PN_002554"
     CDS             788..2008
                     /locus_tag="ACG8PN_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4862670.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2005..4725
                     /locus_tag="ACG8PN_002555"
     CDS             2005..4725
                     /locus_tag="ACG8PN_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862671.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4709..5653
                     /locus_tag="ACG8PN_002556"
     CDS             4709..5653
                     /locus_tag="ACG8PN_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4862672.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2131 a          911 c         1122 g         1555 t
ORIGIN      
        1 tataaaaata aaatgaaaaa aatttttcac gaggtaggga gtgaattttc tatcaaagag
       61 ctaaactatg agcttgagta tgaaaaatct atttgttcaa tcccttataa taatattgag
      121 gaaattatag aaaaggatgc tatcagcatt tacaaatttt ttatttttat tcctaatcgt
      181 caattgaaag ctaatatatt taaccatttg aaaaagatgc taaatgtgtc tgttactgaa
      241 tcggcacctg taaatattga aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat
      301 cgtttggaat caatctacaa tttaaagaaa cctttgagaa tcgcgatcgg agatagttta
      361 aatgacttat ctatgtttga aagtgcagat attagttttg caatgggaaa tagtcatcaa
      421 gaaatcatga aaaaagctga ttatgtcact gtttcaaatg ataaagacgg agtatctcaa
      481 gcatttgaaa gaattcttac catataaact aattaaggta gtatagaaca tagaattcta
      541 aatacggaaa aatggagcat aaattccagt acaaatagta cttcttcagt gtcaaacgaa
      601 gcgaatataa catagatatt taggattgtg acaaaaatca tgtcacaatc tttttttcga
      661 ataaacttat tttttgaagt cggccattta tcgtaatttc tttgcataat acgaacaaat
      721 tttcggttat tgatgaaaaa aaacattgaa aatggtatga taaaagaaaa aaaggaggaa
      781 atttttaatg gtgaaattta tccatgcggc agatttacat atggatcggt catttgaagg
      841 actgacaact cttgataaaa ctgtacagga aaaactattg aagacgaatc tgaccgtttt
      901 atcaaatatc attgaacagg caataataaa taacgttgat tttgttttac ttgcaggaga
      961 taattttcat caaaatcgtc cttcattaaa aatacagaaa catttttcag aacagatgaa
     1021 acggttagaa aagaaccata tacctgtatt tataatattc ggaaatcatg atttctatca
     1081 aaaagagcgg tattggttct cctttcctaa aaatgtccat ttatttacat ccgaaacggt
     1141 agagacaaaa aaaataacga taaaaagtgg agaaactgtc tctctcagtg gatttagtta
     1201 tcgtcaaccg tggattcaaa aagacaaagt gatggaattt ccttctagag aactcacaga
     1261 ttaccatatt gggttgtacc acggggaacc tggcgtctct caaaaaggaa actatgctcc
     1321 tttccaaccc tcaaagatgc aagaaaaggg atacgattat tgggctttag ggcatatcca
     1381 tgtgccaacg gtcttaaatg aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca
     1441 tactaaaaag gaacaagctt ctacatctat tctactagtt gaattaagta aaggaagttg
     1501 ccaaatacat ccgattaaag tagcagaggt ctattggaag acaaaggaaa tatcattgag
     1561 gaaagcaaga acgacaaaag aggtcattgt tcacattcgt cagcagcttt caaaagtaga
     1621 ggatggattt agtttaattg agataaaatt aaaagattat gaccatctca atacagatgt
     1681 tttggaacgg atacaatcag gagagctatt agactatttg ttagaggaat tctcagatag
     1741 gataaatgat ttctttattt ggcgtatttc tctcatagag aatacttttt atacgaagac
     1801 cccacttgct gcttctgcta aattgatgga acagttattt caatactatc agacacctca
     1861 agatttccaa caaattttaa atgaagtata tagtcatcaa gaagcggcta gaatattgag
     1921 cgaactgccg gaatatcaag aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga
     1981 ttttttattt gaggaggatc aggaatgaag attttaagga tcgagattgc tgcttttgga
     2041 aaatggcgtc aaaaaagttt tgacttttat tcaggcaatc agctgatcta tgggggaaac
     2101 gaagcaggaa aatcaacgat ttatcaattt attcaagcaa tattatttgg tttcccttca
     2161 aaaggccgta aaaaaaagga ttatacacca aaagacggct cagcctatgg cggcaaaata
     2221 tggcttaaac atcctgtata tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc
     2281 ggaaaatcga aggtgtggct gggggatcaa gtgggaagtg atgagttact agaaaaactg
     2341 atcgcacctt tgacgaaaga gttattccag caggtattta ctttccagca agagcagttg
     2401 atggaactag atcatttgag agaaaaagag ctacatgatg cgctgatttc tttaggcatc
     2461 acgggcagtt ctcaagtatt ccaaaagcgg caagaatatt atcagaaagc acaacaatta
     2521 ttcaagaaaa aaggccaaaa attactaatc aatcaaaaat taaatgaatg gaaagagctg
     2581 caagaaaaaa ttcatcaaaa acaagaacag gaagcttatt ttggcaagtt gatccaacaa
     2641 gaacaggaat acgagtcgca acagcagcag ttattggcac aattaaaaga ggcgaacaaa
     2701 caattttcat tagccagaca gcaagaactt aatttttctt tatatgaaga atggcaatct
     2761 ctcaaaaaaa acagcactac aggtcttcct gatgagcaac ttcttttaga tctggagtct
     2821 ttctccaaac ggtatcagca aacaaatgag cgtttggaag aattagaagc aacactggaa
     2881 aagcatagcg ggatggatca gcagtcagcc agatattatt tttatttaga gcaggagcag
     2941 gaactaaaag atctccagca acttcaattt acagcggaac aattaaacga agaatggcaa
     3001 agattacaaa ttgagactag agaaacaaat tcagaattga agattttgga acaaaggtgg
     3061 cattggcaga aaaatccgtt accggaagaa tttttatatg aagaagaatg gaagcatctt
     3121 ctggctgaag aaagaaggat tgctgatcta gaaagccaga tttctgtgcg tctccaaata
     3181 gctaaagaac agcaagagat gattgaagca gaagtgaatg acttggaaga gacctatcca
     3241 gcattattac aaaatcagtc agagatagca ccagtctctt catctaaacc gtatatgctg
     3301 atggctggcg gagcaataat cattgcaggt atatttgtac caatgccctt gaaagtgatc
     3361 cttttactaa ttggtgcagg catgattggc tatttcttct acaaaaggca accaaaaatt
     3421 aaaactaaaa atgatttctc tgagataaaa ggaatgtggc aagaaaaact aggacaactt
     3481 gatctagcct tagctcaatt agctgaagca gaagaaaaac aaaagagaac tggagaagaa
     3541 aaacagattc ttcagcaaga agtatatcgt tttgcggatc atcggcactt aggaaagatg
     3601 gatacgctca aactcatgaa agaatatgga caggaagtca tagattatca gaaattatat
     3661 ggcgtatttg agcggaaaca taaaagagaa caggaaatac aagaaaaact acgacaaatc
     3721 gaccaaaaat ttgagtttgt taaagaatgg ttaccgatac aagataagac aatttcagaa
     3781 aaaatggtat tgctttcccg ttttatacaa gagatggaac agatgaaatt tgccagaagc
     3841 tatcagcaaa atactttatt gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta
     3901 tggatacaag aatacagcga acaattgact caagcaggct tatcttatcc ttcggaagta
     3961 cctgtctttt tacaaaaagc caaacaagcc gaacagcaga tgaaaagata caatgaatta
     4021 tcggaaatgc ttactccact atttcctgaa gagctgacaa aagagaaatt atctgtaaga
     4081 ctgcgccaac tgaaagaaaa gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa
     4141 aatcggcaac gactccaact tcaagtagaa gagttgcaaa gagacggcac attggacgaa
     4201 ctttatcaac aagctagccg gcaaaaagca aaattacagg aattactgga ggaatgggga
     4261 gcacttgaaa tcgccgggac attcttaggt gatctttcaa cagagatgag tgaaagacaa
     4321 ttaccccagt tgttaagagc agcaggtgat tatttaggta ttttaacaaa aggaaattat
     4381 cagaagatat tactgaaaaa tgatacactg actttaacag acggatatac agaatttccc
     4441 atctatgagt tatctacagg tacgaaagat cagctgatta tggcgatgag atttgcttac
     4501 ctctcattag aagctaaccg ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat
     4561 tatgatcatc agcgaaagta tcaattagct gaacttttag ctaaatttgg agaaaagtat
     4621 caaataattt gtttttcttc tgataaagaa atggtaagct attaccatga gatgaaccaa
     4681 atcgtaaaac aattagaagg agcaaataat gaaaaaaatt cgtgatttag taatcgatga
     4741 gcagtttgaa caatttgtcc tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa
     4801 aaaatttatt gcttttactt tccaagatac ttcaggtact atcgatggta aatactggga
     4861 tgcttcggag gaggaaatca ataaatttac agccggcaca gtcgtttttc taaacggtaa
     4921 aagagaggtt tatcaaggaa accctcaagt aaagattctt catatgcgct taacaagggt
     4981 agatgagcca ggagagccta gtttatacat ggaaagagcc cctttgaaga aagaagaaat
     5041 ggaagaagaa atcaatcaat tgttgtttga gatcaccaat gcgcattgga atcgtattgt
     5101 tcgctatcta cttaataaat atcaaaaaca attttttgag tttccggcag caaaaagaaa
     5161 ccatcatgca tttgccagtg gattagctta tcatactttg acaatgcttc acttagggaa
     5221 atcaatcgcc gatgaatatc ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt
     5281 gcatgattta ggaaaggtga ttgaactttc cggtcctatg tcgacagaat atacgcttgc
     5341 aggaaatttg atcggtcatc tggtattgat tgacgaagaa ataactaaag cctgtcttgc
     5401 gttgaaaatc agcgaagctg acgaagatgt tctcgtatta cgtcatatgg ttttagcaca
     5461 ccatggctta ttagaatatg gctcacctgt acgtccaaga ataatggaag cagaaatatt
     5521 acatcaaatc gacaatatcg atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac
     5581 agaaccagga aaatacaccg atcggatttt cggtatggac aaccgaagtt tctatgtacc
     5641 aaaagattta tagcaaaaat ttattttttt cattcaaaac aaaaaaagcg agacataagt
     5701 cttcgctttt tttgttttc
//
LOCUS       DAZUKA010000160         5565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000160 DAZUKA010000000
VERSION     DAZUKA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.164"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACG8PN_002557"
     CDS             complement(29..1261)
                     /locus_tag="ACG8PN_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4862673.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACG8PN_002558"
     CDS             1516..2085
                     /locus_tag="ACG8PN_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4862674.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACG8PN_002559"
     CDS             complement(2263..2703)
                     /locus_tag="ACG8PN_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4862675.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACG8PN_002560"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACG8PN_002560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4862676.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACG8PN_002561"
     CDS             3657..4580
                     /locus_tag="ACG8PN_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4862677.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACG8PN_002562"
     CDS             complement(4805..5044)
                     /locus_tag="ACG8PN_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862678.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACG8PN_002563"
     CDS             complement(5049..5468)
                     /locus_tag="ACG8PN_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4862679.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
BASE COUNT         1706 a         1051 c         1038 g         1770 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acaca
//
LOCUS       DAZUKA010000161         5496 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000161 DAZUKA010000000
VERSION     DAZUKA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.165"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      29..195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            343..900
                     /locus_tag="ACG8PN_002564"
                     /pseudo
     CDS             343..900
                     /locus_tag="ACG8PN_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            958..2997
                     /locus_tag="ACG8PN_002565"
     CDS             958..2997
                     /locus_tag="ACG8PN_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4862680.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSANKRASELLQYLNQNYQ"
     gene            complement(3106..4257)
                     /locus_tag="ACG8PN_002566"
     CDS             complement(3106..4257)
                     /locus_tag="ACG8PN_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4862681.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4411..4941)
                     /locus_tag="ACG8PN_002567"
     CDS             complement(4411..4941)
                     /locus_tag="ACG8PN_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4862682.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5040..5390)
                     /locus_tag="ACG8PN_002568"
     CDS             complement(5040..5390)
                     /locus_tag="ACG8PN_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4862683.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         1994 a         1011 c         1036 g         1455 t
ORIGIN      
        1 ctatcatgaa ctcgtaaata gaatatagta ataaccttgt taggtgaggc tcctatacaa
       61 acataggctg ttgcccagaa acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga
      121 ttaaggcttt ttccaatagc taagaatgtt ttctttacgt tgtatagtgc taaaactcgg
      181 acgatgagat gatgctaggt cagaattctt gttcgaaaag gtttctgacc ttttctttat
      241 atttttgtga ctgacttatt tgatgagaca aactcgctcc tcacctgaca agtggattgc
      301 tgtgtgagga ggagatgcgg tgaaaaaaga tcgattggtg gaggtcgctc ggattcactt
      361 ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat catgagagat
      421 tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta ctcttatggg
      481 ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc tccctattat
      541 gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc tggtggagtc
      601 aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa aggagagcaa
      661 gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa agaaggagac
      721 ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca gctgaaagac
      781 gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag tatccaaacg
      841 aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga catatgttag
      901 aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga ggaaaaaatg
      961 aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg cgcagtgatc
     1021 ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga aacccaagca
     1081 gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa gggagattat
     1141 aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt atctgaaaaa
     1201 gagatcttag atcaatacca gaatatatac ggtgctgctg atgtcaaagg acttcagata
     1261 tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata taaagcaaag
     1321 atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt agacagaaat
     1381 gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat ggaaggaaat
     1441 gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga tcgaaatggt
     1501 gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag taaacttgga
     1561 gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga cttaacagaa
     1621 gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt tgtcccattg
     1681 aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca agaagtagac
     1741 ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt gggagatatc
     1801 acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat cggtcgctca
     1861 ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgagaa attaagcatt
     1921 acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca aaatggcaaa
     1981 gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag tctaggagga
     2041 aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc gcttgctagc
     2101 tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga ttacaaagct
     2161 tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta tgctcctgga
     2221 tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat cgatccaaat
     2281 gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg atcttttaaa
     2341 acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt gatctattcc
     2401 gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt tcgtacaggc
     2461 ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa tccagcacaa
     2521 atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg atatggacag
     2581 ggcgaacttc taattactcc tatccagcaa gcagcaatgt attctgtttt taccaacaat
     2641 ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa gaagaatgtc
     2701 atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt gcaagatgta
     2761 aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa aactggtaca
     2821 gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt gtttgctttc
     2881 aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga tgatgatgat
     2941 tcagcaaaca aacgagcatc ggaactattg caatacctca accaaaatta tcaataaaaa
     3001 attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct tttttcaaat
     3061 aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca cttcataaac
     3121 tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat
     3181 aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat
     3241 gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg
     3301 agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac
     3361 aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa
     3421 tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg
     3481 gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat
     3541 gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga
     3601 tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc
     3661 aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa tcaaaacaag
     3721 cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga aacttagaac
     3781 taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc cttcttttgt
     3841 attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag ttgccgcaag
     3901 aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc ctgtcaacca
     3961 gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag gtcggaaata
     4021 ttttttcaaa agagataaat cactttccag accagctaaa aacattaata agaacacacc
     4081 aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc cagcttggcc
     4141 tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc tccgcgagat
     4201 atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa attccattct
     4261 catcattcct ttgtataaaa tatagcccga acttagcata acatacatta ttgaaaaaat
     4321 aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt
     4381 tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat
     4441 gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag
     4501 aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat
     4561 actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa
     4621 gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc
     4681 aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact
     4741 ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa
     4801 aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac
     4861 aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt
     4921 ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag
     4981 tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat
     5041 caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct
     5101 aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca
     5161 acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg
     5221 aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa
     5281 caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac
     5341 ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac
     5401 ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg
     5461 attattcaaa aaaacaaaaa ggattgtgac aaaatt
//
LOCUS       DAZUKA010000162         5485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000162 DAZUKA010000000
VERSION     DAZUKA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.166"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1557)
                     /locus_tag="ACG8PN_002569"
     CDS             complement(52..1557)
                     /locus_tag="ACG8PN_002569"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4862684.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="ACG8PN_002570"
     CDS             complement(1649..2239)
                     /locus_tag="ACG8PN_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4862685.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="ACG8PN_002571"
     CDS             2418..4064
                     /locus_tag="ACG8PN_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4862686.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="ACG8PN_002572"
     CDS             4113..4832
                     /locus_tag="ACG8PN_002572"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4862687.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..>5485
                     /locus_tag="ACG8PN_002573"
     CDS             5311..>5485
                     /locus_tag="ACG8PN_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4862688.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
BASE COUNT         1653 a         1002 c         1107 g         1723 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat
     5461 atttcagcag atgggttctt ggcta
//
LOCUS       DAZUKA010000163         5326 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000163 DAZUKA010000000
VERSION     DAZUKA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.167"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..323)
                     /gene="prgO"
                     /locus_tag="ACG8PN_002574"
     CDS             complement(48..323)
                     /gene="prgO"
                     /locus_tag="ACG8PN_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4862689.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(295..1248)
                     /locus_tag="ACG8PN_002575"
     CDS             complement(295..1248)
                     /locus_tag="ACG8PN_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862690.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1860..3353
                     /locus_tag="ACG8PN_002576"
     CDS             1860..3353
                     /locus_tag="ACG8PN_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4862691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3488..3784
                     /locus_tag="ACG8PN_002577"
     CDS             3488..3784
                     /locus_tag="ACG8PN_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4862692.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3885..4736
                     /locus_tag="ACG8PN_002578"
     CDS             3885..4736
                     /locus_tag="ACG8PN_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862693.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4757..4969
                     /locus_tag="ACG8PN_002579"
     CDS             4757..4969
                     /locus_tag="ACG8PN_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862694.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1820 a          825 c         1001 g         1680 t
ORIGIN      
        1 gtaagtatac taaatatatt tactatatct agtatactta ctatatttta atcttttgat
       61 cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc gtccattgca
      121 agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg aatggttttt
      181 agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt tcgttcccct
      241 aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt cttcttatct
      301 ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta ggatatgatt
      361 ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac catgttgttc
      421 atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag cccaccatct
      481 tcgatctaca taatcattta attggatacc attctcacca tacatttcta accgatccga
      541 gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg ttttttgata
      601 gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc caatgatttg
      661 aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa taaaatcttc
      721 tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat ctgcagcagt
      781 taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga tataatcgaa
      841 attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac gacgatcagg
      901 atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag ccataatata
      961 taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga ttgctgcaga
     1021 catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag tagtatttct
     1081 ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag aaacaatcgc
     1141 taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa gcattacaac
     1201 ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag ttccctccaa
     1261 tttcctatac taaatatatt tagtatactt catatactta atatattcga tatatctaat
     1321 atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat actaaatata
     1381 tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg tgcgttttgc
     1441 gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca gataattcac
     1501 tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca taaaaaaaag
     1561 agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg ttaggacatg
     1621 tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt aaaagttgat
     1681 cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact caacagaaga
     1741 acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga acacatacta
     1801 tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg ttacaatcga
     1861 tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac aaatatcgaa
     1921 acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct atctttgcgt
     1981 accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg gaagaagcta
     2041 ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc tttggtactt
     2101 atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta agtcaaatca
     2161 acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt attttagcct
     2221 gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat ggttatcaag
     2281 tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc aaggtgatcg
     2341 atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa ttgtccggag
     2401 tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat aatattgttt
     2461 ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg atgaagcttg
     2521 aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt cctgagaaaa
     2581 aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa gcaaacatcg
     2641 tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct ttagcttatt
     2701 actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc aacgaacggt
     2761 tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct tattctggta
     2821 attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg gtttcaccaa
     2881 ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt aaaaagccac
     2941 gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg gcttatctaa
     3001 gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa ttggcttcac
     3061 aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga gaggagaata
     3121 aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct tcagtcaaaa
     3181 cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat tcaacaagga
     3241 tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga caattatcaa
     3301 cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt tgaaaaataa
     3361 aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt gctccctttg
     3421 cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt atcatggagg
     3481 aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt ttatcattgg
     3541 tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc aggggacttt
     3601 gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg
     3661 gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt gtgtgttaga
     3721 gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga agtatatcca
     3781 gtagtagttt ttatttactt aaaaaattgg ttcattgagc aacctatcgc tgctaaagta
     3841 gcaaaggaaa acgttaaagg aaagaattta gggaagaaat cgaaatgaaa tcggacgact
     3901 ggattgaaca cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa ttttgggtat
     3961 tgcaattatg gccattagga aaactttgta gttacggggc acgacttatt cttgatttaa
     4021 ccgtttactc gattggagta tttggaacgg cttattactt gataaatgat cccaatagga
     4081 ttgcagcatc agacattttt acagtgctaa tgacgttgat ttttgtcatg ctattgtcta
     4141 tttttactca attagtcatg caatttacca ggcaaaaatt aacagcagga aaacttttca
     4201 aaagcctatt cgatttaatt gttgtaaatg caattctagc gactttaata aaacaaattg
     4261 aacaatctgg aagaacaaca aaaggagtga aggatttttt attgacgttt attcactttg
     4321 taaatggaca catgtatttg tttgaagtgg gtattattct cttttttgtc gggtatgtat
     4381 ccacgaaact tattaaacgt tcaattgaaa aagaaattga aattgacctg attccgacac
     4441 ttcttggtta tgagtacaat gttgattttg gaagatatga cgaattgatc aatacggata
     4501 tcgttcacac gagtatccat gaatctactg ttaagggcat cagatctatt ttaaaaaatg
     4561 gccaggaaat gaaagcaata gatcgaagaa aagagatcta caatttttat gaagtcgaag
     4621 caagcctacg atatggcatc ggaaaattgt ggttagatgg aagtaatatt cgtcaaaaca
     4681 aaccatacat tttttggaac agtagtgatt tagaacttcc aatgaaagat aaataattat
     4741 ggagaatcgt ttgaaaatga ttcctctata caggattgtg cttatagttg gcatggagtt
     4801 aaaaacctta aagaaggagc agttattcaa agtgaaagaa cgtcataatc agttactatg
     4861 tagtaaaacg ggtacgatga acattgaata ccgaacagaa tatttagaat taacgaatca
     4921 aactgatttt ttgttgattc gaaagcaaca agagcaagaa aaaaattaat ggctacttat
     4981 agccaattga ttgatgaatg ggaacaattc aaggaatcta aaattccaga acgtctctaa
     5041 aagcctttat ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc
     5101 aataggctgt gcctattacg tttctcaaaa ataaatgtgg gaacaaaata tttaaaaata
     5161 ggatagtgaa aggatagtga aggatagtaa aaggagggta gtaggatagc aaaaaggata
     5221 ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa gaataagaaa
     5281 tgtggatgag aaaatcgtaa gagaaattga tcgaagatgt gacgaa
//
LOCUS       DAZUKA010000164         5275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000164 DAZUKA010000000
VERSION     DAZUKA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.168"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..263
                     /locus_tag="ACG8PN_002580"
     CDS             <1..263
                     /locus_tag="ACG8PN_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862695.1"
                     /translation="KKKNPAQTSCLFFSNRFAHEINCFSHHRQFIPFTSFNLTATRHK
                     GVIATLSHFAYSSRLRSFVKVKTVVQGAVGIGVKACCSKFLD"
     gene            439..537
                     /locus_tag="ACG8PN_002581"
     CDS             439..537
                     /locus_tag="ACG8PN_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4862696.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
     gene            complement(691..1188)
                     /locus_tag="ACG8PN_002582"
     CDS             complement(691..1188)
                     /locus_tag="ACG8PN_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862697.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRV
                     VFALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQ
                     ALKIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYF
                     ELNSQ"
     gene            complement(1216..1671)
                     /locus_tag="ACG8PN_002583"
     CDS             complement(1216..1671)
                     /locus_tag="ACG8PN_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862698.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGY
                     IKVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQ
                     VQKFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
     gene            complement(1679..2665)
                     /locus_tag="ACG8PN_002584"
     CDS             complement(1679..2665)
                     /locus_tag="ACG8PN_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4862699.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLA
                     QTQLEATRRTFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPN
                     HEIYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQ
                     PKEKFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNK
                     GKRIGFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVT
                     NDDFSISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
     gene            complement(2650..3006)
                     /locus_tag="ACG8PN_002585"
     CDS             complement(2650..3006)
                     /locus_tag="ACG8PN_002585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4862700.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLC
                     KSVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQ
                     QLSQIEKGLIDLWEQL"
     gene            complement(3562..3927)
                     /locus_tag="ACG8PN_002586"
     CDS             complement(3562..3927)
                     /locus_tag="ACG8PN_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862701.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFM
                     RNASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLD
                     YKTFYPELLEFFQKLNSLE"
     gene            complement(3936..4097)
                     /locus_tag="ACG8PN_002587"
     CDS             complement(3936..4097)
                     /locus_tag="ACG8PN_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862702.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLT
                     EIIELDSKE"
     gene            complement(4087..4236)
                     /locus_tag="ACG8PN_002588"
     CDS             complement(4087..4236)
                     /locus_tag="ACG8PN_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862703.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGE
                     QKNDG"
     gene            complement(4250..4999)
                     /locus_tag="ACG8PN_002589"
     CDS             complement(4250..4999)
                     /locus_tag="ACG8PN_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4862704.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVL
                     DYCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVE
                     RPDGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGK
                     YALIMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELS
                     DKMSVSFFVTTLKRGRKVVGYEITINDTSKE"
BASE COUNT         1373 a         1195 c          976 g         1731 t
ORIGIN      
        1 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
       61 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
      121 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
      181 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
      241 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
      301 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
      361 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
      421 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
      481 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
      541 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
      601 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
      661 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
      721 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
      781 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
      841 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
      901 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
      961 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     1021 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     1081 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     1141 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     1201 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     1261 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     1321 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     1381 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     1441 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     1501 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     1561 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     1621 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     1681 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     1741 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     1801 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     1861 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     1921 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgccctcat
     1981 catcgaccca tttgaagcca atccttttgc ctttattgta ctcataaacc tcgacagcat
     2041 aggcttcttg aagatttttt ttgaaagaat caaaatcggt actcatatct tgtgcgccca
     2101 ggatcatatg cttcattttt tctcgattac tcattttgcc ctcggcgtgc atttcaaaat
     2161 caacttgttt gaatttttct ttcggctgtt caatgccaaa actcaataaa accttatcgt
     2221 ttaggtcttg taggtggtag ccgacctcgg tatcagagtg aaatttttta ccggtttcag
     2281 tgtcgatact ggcgaaaaca atgtgattat gaatatagcc ggattctgaa tcggtatgag
     2341 tgcccatgta gatttcatga ttgggatagc tttttgcgat ttcatcaacg agtttagcac
     2401 cgatctcatg cgctttttgc acgtccgccg gattgttttt gtcaaacgaa ttatcaaacg
     2461 attgaaccca atgaaaggct tgaataccgc ctgttttggc gaaagtccgt ctagtcgctt
     2521 ctagctgagt ttgagccaat ttattctcag ctgacagttt gtgcgtataa atgcggtcac
     2581 gatcaagctt tccatgccga tttacatagc caatcagcgc agaagcccca cgtttaccgc
     2641 cttttaaagc taaagttgct cccataagtc aatcaacccc ttttcgattt gcgaaagttg
     2701 ctggttcatc aaggctaaat ctgaacttga aggcatttgc cccttattca tgttaataaa
     2761 gcgagccatc tgattgagat ttccgccata tttgcgcaac tctttggcaa tttgtatcgc
     2821 cccctcccga tcaacgttcg gcgctcgata cttcatgcca agtgccacgc tcttgcagag
     2881 ctgtggcaca ctcataccag tttgatccgc aatggcggtc aaatactggt actcgtcttc
     2941 gctcactcga aatttaattt gccgactatc ttttcgattt tcggcattca atttctgctc
     3001 gctcatctca tcactcctca aataaaaatg ctccctattc ggtcgctttt ttcttctcgt
     3061 cattcttcgt ggtcgatatt caaagctaca aaaacgctcc ctattcagtc gccttttttc
     3121 cgcaatgaaa tctccctcgg ttatgccact acaaaatgaa agttccctac tcggtcactt
     3181 ccctttttcc gctctataac cgaatgattt attaacctca tcatactccc tattcggtcg
     3241 cataacaagg ttaataaatc atataatacg caagcaccgc atttggtacc caaatacatc
     3301 gcttgtcgag gtggcgttgc cccctacgaa ctcccccaat cacgcaacaa aaagtagcct
     3361 gttcggctat ttttattacg tgatttcgcc tcagaaaaag tagtctgttc gactattttt
     3421 ctactggctc atgcggtcaa acttgcacgc aagttgacct tatttttttg tctgcccgct
     3481 gttcgcaggc aaacaaaaaa gcaagccaaa aatcggcttg ctcgttcttc aaaaattgat
     3541 cgcatggact agctcgttcc gctattccaa gctgttcaat ttttggaaga actctagcaa
     3601 ctcaggataa aatgttttgt aatccaactt gtttccccag ttgtcttcca tccatttttc
     3661 gagttgcttt tgaaattgca ccgcttcggc tttcagctcg ctaggcaatt tttcggcaga
     3721 agtggccacc ggctttgcag ctgctggcgc aggtgcagat ccggcggacg acttcaatgc
     3781 ctttgacgca ttccgcatga aagtattgta ggcggaatag ctcttgaacc cttgtttttc
     3841 aaccatattt gtaccgattt tgactgaaag atcatgccga gcttcagcta attttttttc
     3901 caaatcttcg atatccattt ttgccatgcg taaactcact ccttagaatc tagttcgata
     3961 atttccgtta gaagaagatc aaattggttg atcgtttctt ctgaaagtcc accggtgtat
     4021 tcaataatag cgtcactatg cctttctaac agcttttcaa gctgttcaac gtcaatcgta
     4081 actgtcttag ccgtcatttt tttgttctcc tttttcgtca taactatgaa tcaaaaagcc
     4141 gtcacgttcg ccttttgcat agttcattag ttctttcata gcttgatcta gcgaagaatt
     4201 tttctttgcc atctttttac gcagtctttt tttcaactaa atcactcctc tattctttgc
     4261 tggtgtcatt gatcgtgatt tcatatccaa cgacttttcg cccacgcttt aaggttgtga
     4321 cgaaaaatga aactgacatc ttatcagata attcttcaat agcacgagta attacttttt
     4381 gaaaaaattg acctgcagga aggcgtttgt ctttaccaag aaaccaagat tcccattctt
     4441 ccaaatcacc tgtaatcgtt gtgtgttttt cttttcctct acggtaggat tcccagagtt
     4501 ttaacattat gagagcatat ttcccattga tccgagataa ttcacctaaa cgaaagctat
     4561 aaaacttttc tttgagatca agaacgtatg gagctgaatc agcagaaaat ttgaatctta
     4621 ctaacccgtc agcataaaaa tcaatccaag aaaataattg cgtcattctg agaccttgag
     4681 taccatcggg gcgctcgaca cgaagataaa gcgcagtatt ttcattaagc tttttgaaag
     4741 cttcagctat tcgacggtaa gaatctccag aggtgttcaa cccaaaatgt ttgataattt
     4801 ctaatgcggt tacctcgtat tcgtcatcta gttttgaatc tttagttaca aagctaaaac
     4861 agtagtctaa aactcgttgc tcaaacgatt taagccctcc aaatgcctta gctaagtcgt
     4921 ttccttgcac tactaaataa tcttgacggc ttagtaagtc ttttaatgca atatcagctt
     4981 ttttatttgg ttgtcccaaa ctgaaaatcc tccttttgta ctttttttaa aataatacca
     5041 tttattcagt ttttttgcaa tatttgaatt ttcaacgtgg aaaatagtat ttttgacgtg
     5101 gaaaatagta tttttgacgt ggaaaatagt attttcaacg tggaaaatag tatttatagg
     5161 cgctacaaac gctggtataa agccattcct tttcccctaa agtattttaa agtatttaaa
     5221 agtaatctat aaagtattct ctttaagcgc gcgggtgaga aaaaatttta aattc
//
LOCUS       DAZUKA010000165         5275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000165 DAZUKA010000000
VERSION     DAZUKA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.169"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG8PN_002590"
     CDS             <1..546
                     /locus_tag="ACG8PN_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4862705.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACG8PN_002591"
     CDS             complement(716..1678)
                     /locus_tag="ACG8PN_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4862706.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACG8PN_002592"
     CDS             complement(1680..3119)
                     /locus_tag="ACG8PN_002592"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4862707.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACG8PN_002593"
     CDS             3304..5250
                     /locus_tag="ACG8PN_002593"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4862708.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1692 a          907 c          886 g         1790 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaggca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atgga
//
LOCUS       DAZUKA010000166         5271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000166 DAZUKA010000000
VERSION     DAZUKA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.170"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACG8PN_002594"
     CDS             32..388
                     /locus_tag="ACG8PN_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFS4862709.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACG8PN_002595"
     CDS             404..754
                     /locus_tag="ACG8PN_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFS4862710.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACG8PN_002596"
     CDS             783..1562
                     /locus_tag="ACG8PN_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFS4862711.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACG8PN_002597"
     CDS             1573..2235
                     /locus_tag="ACG8PN_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFS4862712.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACG8PN_002598"
     CDS             2254..3360
                     /locus_tag="ACG8PN_002598"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFS4862713.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4440
                     /locus_tag="ACG8PN_002599"
                     /pseudo
     CDS             3350..4440
                     /locus_tag="ACG8PN_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
     gene            4453..5193
                     /locus_tag="ACG8PN_002600"
     CDS             4453..5193
                     /locus_tag="ACG8PN_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFS4862714.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1691 a          864 c         1255 g         1461 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacaggtg acatgtacca tgtctatcat ccctatacag aaaaaatcgc tagacgggaa
     3661 atcatcatac ctaagggaca taatgtagat tatgggacac cggttgaagt aatggttgct
     3721 caaggtggag gaaaagtcat agaagctggc tatgcaaata tgtgtacacc ggaacatgtt
     3781 tccatgatga ttacggaaaa aactgctgcg cttctatata ttaaaagtca tcatgcagtt
     3841 cagaaaagta tgctgacggt ggcagaaatg gtagaagttg ccaaagctca tcgtttgcca
     3901 ttgatcgttg atgcagctgc ggaagaagat ttattcaaat atagcgaaat gggcgttgac
     3961 ttagtgattt actctggggc aaaagcaatc gaaggtccga gtgccggact ggtcatagga
     4021 aaaaagaagt atattcaatg gattcggcta cagtctaaag gtattggccg agcaatgaaa
     4081 atcggtaaag ataatatttt aggctttacc caggcactag aagattattt aaaaaatggg
     4141 agcgaatccg gtgactccat gaagcaacga ttagcgcctt ttgttaacgc aatcaatcaa
     4201 cttcctgacc tttcagcgaa aatagttcaa gatggtgccg gcagagatat ttatcgagca
     4261 agtgtaacga ttaatggcga taaaagtgcg aaagaagtca ttcaggaatt aaaagcacga
     4321 aatccagcag tctacacccg agaatatcaa gcaaataacg gaatcattga gttcgacatt
     4381 cgttccgtca gccaaaacga aatggaaaaa attataaaaa gattgagaga aatcatgtag
     4441 gaggctttta aattgtcagt tatcccaaat tattataaaa atcgtgtatg tttaaatgta
     4501 ttagctcatt cagttgaaaa tgcaaaagca tgctatgaag cagcggaagg gcatgtcgta
     4561 ctaggtgtgc tatcgaaaaa ttatgctagc gatgaagcag caatcgaaga tatgaaaagg
     4621 taccaagacg caaccaataa tgcgttgtca gttggattag gcgctggaga tccaaaccaa
     4681 agtcagatgg tttcgcgttt atctggggtt ttacaaccac aacatgtcaa tcaagtattt
     4741 actggcgtgg ggacttctcg tgctttattg ggccaaaatg atacagtcat caatggattg
     4801 gtttcaccaa caggtaagat cggctatgtc aatattgcta caggtccgtt aagttcacaa
     4861 gcgccagcag ctgaagtgcc tattgaaact gccattcaaa tgctgcaaga catgggaggt
     4921 acttcaatca agtatttccc gatgaaaggc ttagcacata gggaagaatt taaagcagtt
     4981 gcggaagctt gcgcaaaata tgaattctat ttagagccaa caggaggaat tgatctttca
     5041 aactttgaag aaattctgaa aattgcaatc gatgcaggtg taaaaaaagt gattcctcat
     5101 gtttatagtt caattataga taaagaaaca ggagatacgc gtataggaga cgtaaagata
     5161 ctattaagca tggtaaaaaa aattattcac taactcatta gaaaataaga agcttattcc
     5221 agaatgaaat taaatggaat aagcttttta taattaatta cggaaattgt t
//
LOCUS       DAZUKA010000167         5254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000167 DAZUKA010000000
VERSION     DAZUKA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.171"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..459
                     /locus_tag="ACG8PN_002601"
                     /pseudo
     CDS             110..459
                     /locus_tag="ACG8PN_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(731..1021)
                     /locus_tag="ACG8PN_002602"
     CDS             complement(731..1021)
                     /locus_tag="ACG8PN_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862715.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1423..1659
                     /locus_tag="ACG8PN_002603"
     CDS             1423..1659
                     /locus_tag="ACG8PN_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862716.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1666..3618
                     /locus_tag="ACG8PN_002604"
     CDS             1666..3618
                     /locus_tag="ACG8PN_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4862717.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3690..4187)
                     /gene="dfrG"
                     /locus_tag="ACG8PN_002605"
     CDS             complement(3690..4187)
                     /gene="dfrG"
                     /locus_tag="ACG8PN_002605"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4862718.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4514..5206)
                     /locus_tag="ACG8PN_002606"
     CDS             complement(4514..5206)
                     /locus_tag="ACG8PN_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4862719.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1695 a          912 c          867 g         1780 t
ORIGIN      
        1 ctattatgtt tgttcatttt tatactcctt cttatatttc ctaatagagt ataacatagc
       61 tttaagcatt acttaaagat aacttaaaag ttatctttta aatacttaat tgttaaatac
      121 cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa
      181 aaatttttct tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg
      241 ccaaagacat acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc
      301 tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag
      361 gcttcttcaa tttactttta cgactatgat aatcacctct tcgagttaca taccggtact
      421 ctccaggaac gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct
      481 ttttatggcc tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt
      541 caatctttaa gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac
      601 ttaaatacat gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc
      661 aaatcccatc gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc
      721 cgcttgtttt ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt
      781 tctcatattc taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt
      841 gctttctatt attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta
      901 ggaagttcaa actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca
      961 acggtctggc actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca
     1021 taaccttcac ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg
     1081 ttttatcttt ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg
     1141 aagatgaagg acatgtacta aaaaaaagag gcagcagtat agatttaagt agttcagttt
     1201 tggagtacaa aaagtgaaaa tgtaaaccta aatgagtatc aaattgcaac tgaatttgat
     1261 actcgttttt tattgttaaa tcaacgtttg ttgttaattt acaccacaaa atgtaagtta
     1321 aaatgtatat aaaaatgcga acgtgaaata attattgtag gaatgcaaat tggtgtgtta
     1381 aaatggttac aatttactaa gcaaaaaggg gtgcattttc ctatgcaatt tcttgagcca
     1441 aaaaataaga acgctaagtc tgtggattgg gaaatatcag agcaagtaag ggtgatagtt
     1501 aagcaatacg ctgaatatgc agagcgtact gaaagcgaag cagtggatga attcttattg
     1561 aacattttag acgataagaa gtttattgaa tggattgcca acaagcgaag taataagcgg
     1621 attgttgaaa aaatgggtat caaagataga gtgggttgat agacaatgac caagcttaaa
     1681 agattagcca cgaaggaaga tgaaattgta gatgttaaaa ttccgatttc aaacgaagaa
     1741 ttaaaagata gggctaaaca atatgattta ttaactccaa aacgatttgc aacaaggtat
     1801 aacaaaatgt tgttcttgcc aacgtctttt aaatggaacg gttctgaata tcccattcaa
     1861 tataattact gcatcaatcc gttttgttgt aattttggga aagaacagca caaattcaaa
     1921 gatgtaaaag ggaaaccaag tcgatataaa atgactggta gttccaaaga taaaggtcat
     1981 aaaggtatgt actgcaatga caatcctatt ggcagaggtg tatcgcaaaa ctgtacagta
     2041 acccctttat ctaattggtc tgttgttgaa gaaattaaac gccttataga aatcaatagc
     2101 atccaagatg ttgagccaga ttatcaattt cataaagaag gctgctcgga agaagaatca
     2161 accccattca atgagccgaa gcaattttac aaaaggggga aaagtagggg caaatctcaa
     2221 cgctatcaat gtaaggcgtg taagaagttc actaatgttc taccaaaaag agaagaaacc
     2281 actacttatc accaacaaaa gaacaccatt cttccaatgt tcgctaagat ggttgttgga
     2341 agggtttcag tcagtcgtac ttgtgatatt ttaggaattg gagttggaac ctactatcat
     2401 aagctggagt ggctgtatag gcgttgtttg gagtttttag aacgatatga gacacaaccc
     2461 cttcaaacaa agaaatttaa cgagatgtgg cttaatacgg acaagatgca ctactaccta
     2521 aataacgtcc gtaagaaagg acaaggttca aagaaatata cggggtttga agaccttaat
     2581 atgcaaacgt atatagttgt atctgctgag gtcttatcaa gatatgtgtt ccgttctgat
     2641 gttgcctatg attggaatat ttcaatggat gaattaaatg aagatacaag aaaattcaaa
     2701 gaagaccatc tcaatacttt tagtcgtaaa aatgataggt tagattggtc ttattatcca
     2761 caagaaccat ccgctaacga tagcgaaaat aggaatgctt atcttcacga attagggaaa
     2821 attactaata ggagtagatt tgtcgatggt ttaaatgttg atgctcctta tactacaact
     2881 gctcactatt ggttaattaa acaaatggtt aatgccgacg aatggcgaat gattagtgat
     2941 gatgacttct ctattagaaa cgccttttat agagtattca cgaaagaatt aaggttgagc
     3001 gatgcacatc attttatttg ccaagttaac aagacgaaaa gcagaaaaca atgcttaaaa
     3061 gaattcggac aagcaaaagc agaactgctc gattggggag atatcagagg attcaaaaca
     3121 aagtttctac ggactttggc atctcattat ctaactgaat tactaacaag tcatcaattc
     3181 cacgaagagg ctattagtaa agatggagaa agatatcgca aatatgccga taaccctatc
     3241 aaacatccat tagccactaa ggataaagga ttttattcag ttgattgtag aacggattta
     3301 tctgctctgg aaccaaacga aatcgcaaag atgctattaa atgttaatga ccattcgacc
     3361 aatagcttta ttcaacagat aagacgttat atatcatctc ttgaaagacc attaacaaca
     3421 gctcgtggtg ataagaagag ctatatttac gctaacttca atccgaagta tgctcaattt
     3481 gcgattacaa ttctaagaac ttactacaac ttttgtcgcc cttttaaatc agcggataaa
     3541 aaggttttga cacctgctca aagattaggg attacagata aacaatttga ttggaaagat
     3601 ataatttatt tcaaatgaca aaaagagctt ggagtaatcc caagctcttg tgctttatct
     3661 ttactgttct tcttcaatta acgcacccgt taactcaata agttttttct ttcatataca
     3721 tgaaaatagt agttatacgg atttttatca ttctttatcc ctttttgggc aaatacctca
     3781 ttccattcct cataattcac ttctggaaaa aaagtatctc cttcgaattc atgatgtatt
     3841 tttgtgatgt acattttctc aacataaggg aaaaacaaat tataaatctg ttctcctccg
     3901 aaaataaaaa tttcttcttc gtttttacat aactcaaaaa catcttctat tgaatgaaca
     3961 atttcacaac cattaaaggt aaacccctta tctctcgtca gaataatatt tcttctgtca
     4021 ggtaaggctc ttccgattga ttcaaggttc ttcctaccta atattatcgg atgtcccttt
     4081 gtagtatttt taacatattc ccagtccttg ggaatcctcc aaggaatgtc attctctttg
     4141 ccaatcactc tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc
     4201 tttttaacta atttaccatc taatttctta tttctatcaa aaaacctttt ctcattatcc
     4261 tgcccgttag ttgaataagc aattttttct ataagttata ttatcataat accacaaaat
     4321 cacaaatttt cattcaatta tcattttcaa ttgcatttta taaatcattt tgtatgtaat
     4381 ttggtatgta agttaaaatg aaaaaagtag tccattttga agtacatttt gtcattttgt
     4441 actccaaaac tgaactactt agtatagatt actgttgctt ctttactaat ttaggtttac
     4501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     4561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc cagcttgagt
     4621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt tctacatttg
     4681 ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta ttttgtaatt
     4741 ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc agatgattga
     4801 tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt tgtttgcttt
     4861 gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct gctgccgatt
     4921 gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca tctgcctttg
     4981 aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta atcgcttgct
     5041 ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta tcaatatctg
     5101 tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag ccaattagac
     5161 atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc atagctcctt
     5221 aatccaatac ttttacatgc taaaagtaag tgat
//
LOCUS       DAZUKA010000168         5230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000168 DAZUKA010000000
VERSION     DAZUKA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.172"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..2000
                     /locus_tag="ACG8PN_002607"
     CDS             66..2000
                     /locus_tag="ACG8PN_002607"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4862720.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2457..2873
                     /locus_tag="ACG8PN_002608"
     CDS             2457..2873
                     /locus_tag="ACG8PN_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4862721.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2887..3453
                     /locus_tag="ACG8PN_002609"
     CDS             2887..3453
                     /locus_tag="ACG8PN_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4862722.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3476..4462
                     /locus_tag="ACG8PN_002610"
     CDS             3476..4462
                     /locus_tag="ACG8PN_002610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4862723.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4617..>5230)
                     /gene="scm"
                     /locus_tag="ACG8PN_002611"
     CDS             complement(4617..>5230)
                     /gene="scm"
                     /locus_tag="ACG8PN_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4862724.1"
                     /translation="TTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTS
                     DSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSD
                     STDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDN
                     GNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1567 a          965 c         1164 g         1534 t
ORIGIN      
        1 agtatcaaac taaaagaatt atcatatatt cattatatag atagtctata aggagcggat
       61 gatcaatggc aaagcaaaat attgtcgttg taggagcagg atatgccggt gtttcagcta
      121 caaaatactt ggcaaagaaa ctgaaaaaag aagatgtagc aattactctg atcgatcgtc
      181 attcgtacca tactatgatg accgagttgc atgaagtagc tggcggacgt gtagaaccag
      241 aagcaatcca atacgatctg cagcatttgt ttgctcgtca aaagaatgta caacttgtca
      301 cagacactgt aatcggtatc gataaagaac gaaaaatcgt caagaccaaa ttaggttcct
      361 atccttttga tcagctgatc cttggaatgg gcggagagcc gaatgacttt ggtacacctg
      421 gtgtcaaagc acacggattc accctttggt catttgaaga ttctgtaagg attcgggaac
      481 atattgagaa gacagtagca aaagctgcat tggaaccaga tgatgaactg cgtaaagcca
      541 tgctgacttt cgttgtctgt ggttctggat ttaccggtat tgaaatgatt ggggaactga
      601 tcgactggaa aaatcgccta gccaaagatt ataaattaga tccaaacgaa atcacactga
      661 tggtcgtaga ggcaatgccg acgatcttga atatgttgga tcgaaatgat gctgcaaaag
      721 ctgaacgcta tttgcgtaag cacaacgtgg aattattatt gaatgcacca atcgtagaag
      781 tggctgctga tcatatcaaa ttgaaagacg gacgcaacat tccgactcac acactgattt
      841 ggacagctgg tgtcaaagga acttctgacg ctgctgactt tggattagaa tcagcacgcg
      901 gacagcggtt gatcgccaat gaatatatgc aggcaaaagg ctacgaagat aaaaacatct
      961 atatcattgg tgatttagtc ttttatgaag aattcccagg aacaccgaca ccgcagatcg
     1021 ttcaagctgc tgaacaaaca ggacacacag ccgcagcgaa tatcgtggct tcgatcaaag
     1081 gcggagaaaa gcataaattt aaaggaaact atcaaggatt tatggtttct attggtgcca
     1141 aatggggggt agctaaccta tttgataaga tccatttaag cggattttta gctatcgtaa
     1201 tgaaacatat cgtgaatttg aaatacttct ttgatattcg ttctggttat tatatgttcc
     1261 aatatttaat gcatgagttt ttccatatca aagatgatcg caacgtgact cgaggacatt
     1321 cttctcgtta cggcaatgtc ttatggagtg tgccacttcg tattttctat ggtttagttt
     1381 ggttcgttga agcttccaaa aaaatcattg ggaatggtga agtgttcaaa ccaagtactt
     1441 ggtttggtga aggatcatgg ttcactggaa acagtgtgtt cccttggaca tgggaataca
     1501 atgtaacaga tgttgccagt ggtgcttctg ctgctggtga tgcaaccaca gctgccagcg
     1561 gagcaggggc aagtgccgga gaagcagcga ctcaagctgc acattttggt ttgagttatg
     1621 catacggtga agaaccaatg gctgtgattg agaagtcacc agactggttc acaaagatca
     1681 tgcaagtgat catgccaaac aatgatgtag caatgttctt ccaaaaattc atggtattct
     1741 ttgaaattgc cttggcatta gcattgattg ctggattatt cacatggcta tgcagtgcga
     1801 caacgattgt attggtgatc acgttctgct tatcaggcat gttttactgg gtaaacattt
     1861 ggttcatttt tgttgctttc gcattgatga acggttccgg acgtgcgatt gggttggatc
     1921 gctgggtaat tccatggcta caacgtacac taggtaattg gtggtacggt agaccaagtt
     1981 cccagtacgg caagaaataa atagataaag aacgttattt gaatgcaaga tttcaataga
     2041 aaggggctga gacaaaccgt cacagcctcc tttttatgta aaattaaagg aataagatgc
     2101 ccgagataag cctccatgag caagacacag gtgtcacaac ctctgttaca acctcttgtt
     2161 ataaacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaacttg
     2221 aaaagtaatt ttcgtaaatt ccttcttatt tcttgaagtt gcccacttct gtcacaacct
     2281 cttgttataa acggtgttcc acctgctgct tctcggtaat aagcccaaag aattagaaaa
     2341 catggaaagc tgttttctat tcttcgttct tattactcaa agcaagacac aggtgtcaca
     2401 acctcttgtt tttatgctaa aattgggtga gttaatctgg aaaaaggaga acaaaaatga
     2461 gccttaaaga atttgctgaa aaaagtctgc tcaaaccttg ggatgttttt attattttgc
     2521 ttttgactgt tctttctttt ttaccagtca tgatttttgg tcttcagcaa aacaccgcaa
     2581 cagcagaaaa agaagcggta ctgcgtgtgg atggcacaga aatcaaaaca ttccctttga
     2641 tggctggaga agagagcaac acatatactt acaaagatga acacggagat tataacttga
     2701 tcgagataga tggcgatcga gtacggataa aagaagcaga ttgcgatgac cagatctgcg
     2761 tacgtcgagg ctgggcaacg aaaaatggcg aaaccatcgt ctgtttgcca cataaattag
     2821 tgatcgaagt ccgttcaaca gatggaggtg acgaagacag tctgatttat tagaccttca
     2881 cacaccatga gtaaattaca aaaaatgatt tatatttcgc tacttgttgc acaaggagtg
     2941 atcattggtt tattggaaaa tatgatccct tatccttttt cttttgcgcc tggtgcaaaa
     3001 ctaggcttag ccaatctaat cacgatcgtt gccattttta ccatgaaaaa acgagacagc
     3061 ttcttactac tttggctacg actattcctg actactttgc taggcggaac gatttccaca
     3121 ttcctttata gtatgagtgg tgctttgctt agttattttg gaatgttatt ggtcaagcaa
     3181 ttaggaccaa aacgtgtcag tatcatcgga attagtgcgg ccggcggttt catgcataat
     3241 gtcggacagc ttttgatggc ttctttcatt gcacaatcat ggacagtcat gttgtacttg
     3301 ccagtccttt cagttttagg gattttatca gggattgcaa tcgggatcgc agcgaattat
     3361 cttttacgac atgtgcagac acttcaacga ttccaaaaag attatgagca gggaaccaaa
     3421 caaaaatgga gcccaatgtt taccaaaaaa taatcgtcac acaaggagtt tttttatgaa
     3481 gctacatcca atgtgggaaa actatcctgc tttaagtaaa gaattgacag aaacactaaa
     3541 acggatggaa gaaactgtcc gcctgaaaaa caaacctgtc gaacaagcaa tcaaagaaac
     3601 gatccatgcc ggcggaaagc tgttgcgtcc tgcctatcag ctattgttct cacaatttgg
     3661 accagagcaa gatcgagaca aagccatcgc tttggcagct tctatcgaaa tgctccatac
     3721 ggccacactg atccatgatg atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa
     3781 tatccgtagt cagtttggta atgtcgtttc tgtctatgca ggagactatt tatttgtctg
     3841 ctgttttaaa ctattgtctg attactccac ttccttgaaa agtctccaat tgaattctcg
     3901 cagtatggaa aaagtattga atggcgaatt agggcagatg gatgatcgct ataattatga
     3961 actttctgta aaagaatatt tggaaaatat ttcaggtaag acagctgaac tgttccaatt
     4021 aagttgttct gttggcgctt ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg
     4081 agatatcgga ctttctatcg ggatggcctt tcagattatc gatgatattt tagattatac
     4141 aaaagagagc caggaaatcg gcaaacctgt actagaagac atgcgtcaag gggtctattc
     4201 tttaccgttg atctactctt tgcaaaaaaa caaaccaaag ctactacctt atttagagaa
     4261 gaaagcggca atgacagaag aagatgtcga cgccgtacgc aaaatcgttg aacatacagg
     4321 aggcgtggaa gaagctagga agttagcggc ctcttacaca gaaaaggctt tgaaggaaat
     4381 aaagaaatta cctgccacct ctcttcgaac aaaagagaat ctcttttccc ttacccaact
     4441 gattttggat cgcaaagact gaatagggaa aggttcgata cgtaaataaa tttctcagaa
     4501 ggaaatctac gtgttttttg acaaattgaa ttgtagttct tgtatttatg ataaaaagca
     4561 aaaataaaga gcaacgtccg ttagcgacgt tgctcttttt tatcttgaat atttttttac
     4621 cttttcgatt tcttataact cataaaagtg attcttaaac cgattacaag taatataacg
     4681 cctgcgagtg ttatccaatt attcgattga cttccagttt taggaagtgc tctttttgat
     4741 ttgtttccat tgtcattgct tgttttatta gttaagttaa agttcgtcct gttatccgaa
     4801 cttgtttttg agaaggagac acttgcatga cgtgtatcgc ttgtggtaga cgacgtgtct
     4861 gtactatcgc ttgaagttga tgaatctgag ctatcacttg tggtagagga tgtatctgta
     4921 ctgtcacttg aagctgacgt gtccgtgcta tcacttgtag tggtagatga atctgagcta
     4981 tcacttgtgg tagaggtagt atctgtactg tcacttgaag ctgacgtgtc cgtgctatca
     5041 cttgttgtgg tagatgtatc cgtactgtcg cttgaagctg acgtgtccgt gctatcactt
     5101 gtggtagagg tagtatctgt actgtcactt gaagctgatg tgtccgtgct atcacttgtg
     5161 gtagaggtag tatctgtact gtcgcttgaa gctgacgtgt ccgtgctgtc acttgtggta
     5221 gaggtagtat
//
LOCUS       DAZUKA010000169         5220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000169 DAZUKA010000000
VERSION     DAZUKA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.173"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(326..1300)
                     /gene="sgrA"
                     /locus_tag="ACG8PN_002612"
     CDS             complement(326..1300)
                     /gene="sgrA"
                     /locus_tag="ACG8PN_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4862725.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1496..2335)
                     /locus_tag="ACG8PN_002613"
     CDS             complement(1496..2335)
                     /locus_tag="ACG8PN_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4862726.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2520..3407
                     /locus_tag="ACG8PN_002614"
     CDS             2520..3407
                     /locus_tag="ACG8PN_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4862727.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4001..4695)
                     /locus_tag="ACG8PN_002615"
                     /pseudo
     CDS             complement(4001..4695)
                     /locus_tag="ACG8PN_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(4679..5077)
                     /locus_tag="ACG8PN_002616"
     CDS             complement(4679..5077)
                     /locus_tag="ACG8PN_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4862728.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1660 a         1046 c          770 g         1744 t
ORIGIN      
        1 cattgatatc aataaattct tcaaacaaag tacatactcc tttttatcta ttatttagac
       61 ttattccgac agctacatga ataacgtaca aatagattcg ttactgattt ttcagagata
      121 ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt attttccatt tgtgttcctg
      181 ttatttcctg tttctcaaat atgttagtaa acaaaaatag atctggcaaa gctacaactt
      241 cgccaggcaa tacagcactc tcgaatgaaa tattcactca aaaatctatc gttttattca
      301 attttagatc tgttgctttt attctttatt tcactcgatg gaagagagaa cgaaaatagc
      361 ttgctcctac aatcaaaatg agtaacacac ccatccatgt aattagcttg ttcaaggttc
      421 tactaccagt ttctggtaaa tcgctttttc tattatctgt ttctttcgct ttttcttgat
      481 gatcaattcc acgagaaaca aaatcaggct gttttagcac actttttagg ttgctccctg
      541 catttctctt ttgttcctta gttggtatga taggtaccaa atcaagttca ttatttactt
      601 ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact ctcactactc atgcttcctg
      661 tgctactttc actactcgta ctctccgtac tgctttcgct gctcgtgctt cctgtgctac
      721 tttcactact cgtactctcc gtactgcttt cgctgctcgt gttttctgtg ctaatttcac
      781 tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc tgtactgctt tcactacttg
      841 tgctatccgt actgcttggc ggtccaacaa ctgtccaact gcttggtttc aaattctccg
      901 tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac tttatcttta ctcttgatct
      961 catcaatcga tagttctgca gaaatcatta ttgtatactc aataccagcg gtccattgta
     1021 cgtcttttaa ataaacttcc ccaataactt tttctgaact tccattacca tcccaaccat
     1081 atgttagaac agcatgtaca tcactagcat tcacgctttt gtttggtttg actggaatag
     1141 taatgacttg ttgtgtcatg tcataaacaa tccgatcagt tttcacagta aatgaaagat
     1201 tctcatttgc ccgttcattt gcagaagtac tgattccaat cattgctgca attatactca
     1261 gcccaagaat aacaaataat acggtcgctg ttttcttcat ttctatcact ctccatataa
     1321 ttaccttcta tttgataaag gaattatata aaatgaaaat gatgagaaca cgacagtcag
     1381 ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa tttgtttact ttttcgttta
     1441 atcaatgaca gacttaaaaa agcaagaccg atatcagcct tgctttcttc atcttttatt
     1501 gataaacagc agtgtatttc ttcatccgct ttgttttata tgcatagagt aacaaaccaa
     1561 caatcaagac taacaagaat aatgtagttg atatagatgc ttcagcccca aagtttcccc
     1621 cagtaattat tgtattttgc gattggatca gagtggagaa aaaaccttgt tcttgcgttc
     1681 cgctgactgg tatacccaaa atagctcctt gtgtccaatt ccaagtaata tgagcaccaa
     1741 tagccgtaaa taaacttcct gtactgtaga taagtgcgct gaaaaagcaa cctgctaaaa
     1801 agatattgaa aactgccaga aatgtcatgt taggattcaa tccgtgcaaa agagcaaata
     1861 atgcagcaga tccaatcatt gctattaacg gaccaaatct aagcatctga tgctgaaaaa
     1921 agccacggaa taaaagctct tctgatgccc ccactgcaat aaataaaata aaactcgtta
     1981 tcagcggcac aatgtttgcg tttcctgaat ctaaccgata cgcccccgtc aaatataatg
     2041 gactagtaat aagcagaaac actccaattc caacaacgag acctcccgct aaataccccc
     2101 agtcttttcc tcgaacagaa acaccataat cttctattct ttttccttcg accttactag
     2161 caagaaataa agctagtaaa aaaacagcta aatgtccgac cgtaccggca attgctccaa
     2221 tccattcact atttccaata aacatatccg caagtgcatt attgacaaga attcctaaaa
     2281 caaaaccaac aaagactatt aaaacagatc caatcaaccg aataatcctg atcatctttc
     2341 atccctcctt tatattaagt ttaacaaccc ttttctttct tgtctcgtga aaactatgta
     2401 aatgttctcc atcatagaga gaatagattt ttacgaacta agaatatcta cagcaacctt
     2461 acgaaaatga aagactgtct ccctaattct tctgttagac tgtttttaat aaggaaagga
     2521 tgggaaattt tttgaaaaaa caactactaa ttatgtgcct ttcagcatgt gcaattttac
     2581 tattctcagg ttgtagcaag tccaatgaaa cgattgcaac gtttaaaggt ggaacactta
     2641 cagttcagga tttttatgat aacccaaaag ttaaatcaaa taatcaaaca ctattaaaaa
     2701 aaatgattgt cttcaaagca tttgatgatg tatacggaaa agagatttct tcaaaggaag
     2761 taacaaaaga atataatgaa caaatcaaaa aactcggtcc aaactacaag gaacaattaa
     2821 aagcagttgg ccaaacagaa gagacatata agaaactctt taaacagatg ctggctttcc
     2881 aatacggttt gaaagccaat gtaaaattaa cagataaaga tctagacact gcttggaaag
     2941 aattctatcc agaagttagt acacagatca ttctattttc gactaaagaa gaagcagata
     3001 aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa gcttgttcaa gcttatggaa
     3061 aaaataaaac attaaaggaa actgatggaa aaatgaattt tgattcaacc aatccagaga
     3121 ttcctactga agtaaaaaaa gcagcattta aactaaaaaa cggggaagtt tctgatatca
     3181 ttcctgtaac agatccaacg acttatcagc aaagctatta tctagtaaaa atggtaaaaa
     3241 aacaggataa aggttctaat aaagacaaat acaaatctga gctagaaaaa attgctaccg
     3301 aagcaaaaac tcttgatacg gaattcatgg ataaaacaat ccgtaaagtg atgaaaaaag
     3361 acaatgtaac tattaaagat ccttatgtaa aaaatatatt caaataaaca aatttgaccc
     3421 aattggggat gtggcaaaat ttgagaagtg atttttggaa attccttctt atttttgagg
     3481 caaaccactt ttgccacaac ttcatcgggg ccttttttta tttgtaccaa aacaaatcac
     3541 tggatttgtt acaatcatga ggatataatt taaaaaagtt ttttatttct atcacatgaa
     3601 tcaatgcgcc attaagtaga ttctttcatg cttccctatt ttttcacttg ccgtatcgct
     3661 tcaaaaacaa ctatcataaa ctcaaactcg actgaaaagt tattttaaaa aagactaaaa
     3721 atatcccttc tggacttgtt cttcttattc tgttatacta gagaaacacg aaagatattt
     3781 ttatgcgaca cttttcattt gtttttatga aatttattca aaactggcgt aacttcagag
     3841 ttacaaggat gtattagagg ttagggaata catcgtttaa atgagaaata agtaatagtt
     3901 agtaatagtc acccaaactt taaattaatg aatattgaat cgtcagatag cctcctcgag
     3961 ttttcgagaa ggctatcgtt tattttatgg agaaattgat tcagaagtaa tcagcgacct
     4021 tttttagttc accaatcgtt tctgcttgga aagtttgcac ccaatcttct tggaagcccc
     4081 tcactgcttt atcaatcgag ggcatctcta atacttgatc tagtagctct ttacctaacg
     4141 ttatcgctga gctccagatc gaatggctgt agtgacttgt tcacaatttt tgaaactagc
     4201 tgctaatatt tttgttggtc tttgcgtacg ttcgatctca gtccgcaacg aatcaatcac
     4261 tttcatagca tcaatgcctt gattttgcat cctgttataa taaggtgcaa tataatctgc
     4321 tccattatta atagctaaat acccttgtaa ttctgtgtaa atagctgtac cggtaatcaa
     4381 ataaccttct tttttcaact ttttgatcgc ttgaaggcct gcttgattca ccggcacctt
     4441 gataaacgta tcttttccca gttcttctat gataactcga gcatcctcta tcatttcttc
     4501 ttccgttctt cctattgtct gaatatggat aggaatctgt ccgattattt ttttgatttc
     4561 tttcatatgc gagaagaagt ttgacgctcc ttcttttttc agaattgtag gatttgttgt
     4621 tacgcctgtt aaattcaaaa actgttgaca agaataaata ctttttgtat ttgctgtatc
     4681 aagcatgatt tccatatagc tttcccccat tctgatcctc ttgatcgatt ttaatgattg
     4741 tcccctttct taaatgcggc atttccttat cttctacata cattgttcct ggaagttccg
     4801 gctcatgaga tccatcaaat tttatcgtga tatgtcctaa ttcattcaaa ttagtttgga
     4861 caacatctcc tactgctgtg attacataca tttgtttatc aaaacagagc ttcatatcac
     4921 aagttatttc ttttcttacc tgattcaatt caatcttata acaataatct gttaatgttt
     4981 ccggtgcttc atctccaaat aaaatcatta ttttttcttt cctgaatact tctgcttcta
     5041 taccaacatc tgttacaacc gtttcaaaga tcgtcatctc tgttcctcct cgtaaaatat
     5101 catttgtcta atagttttta ttcaaaatgt ttctagatta taaaatttat tgaataacac
     5161 attcctaacg gaatttgttt cgatattgtg tgatttctca gagtacaatg gatgtatgga
//
LOCUS       DAZUKA010000170         5159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000170 DAZUKA010000000
VERSION     DAZUKA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.174"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..640
                     /locus_tag="ACG8PN_002617"
     CDS             <1..640
                     /locus_tag="ACG8PN_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4862729.1"
                     /translation="LYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNST
                     IYVGAGTTILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIG
                     EVAERSFESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLN
                     KSDVYTFRKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            606..1559
                     /locus_tag="ACG8PN_002618"
     CDS             606..1559
                     /locus_tag="ACG8PN_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFS4862730.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(1957..2931)
                     /locus_tag="ACG8PN_002619"
     CDS             complement(1957..2931)
                     /locus_tag="ACG8PN_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4862731.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3046..3480
                     /locus_tag="ACG8PN_002620"
     CDS             3046..3480
                     /locus_tag="ACG8PN_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4862732.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3605..4627)
                     /locus_tag="ACG8PN_002621"
     CDS             complement(3605..4627)
                     /locus_tag="ACG8PN_002621"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFS4862733.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5019..>5159
                     /locus_tag="ACG8PN_002622"
     CDS             5019..>5159
                     /locus_tag="ACG8PN_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFS4862734.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1599 a          997 c          926 g         1637 t
ORIGIN      
        1 gctatatggt ggcgttgaac gaaaggaacc ttataatcga gaactatcga ctcaagaaaa
       61 gattgatacg aatgtagccg aaaaaaaata tattggtcaa gtcatgaaca gtatgattcc
      121 cgataattct actatttatg tcggtgctgg aacaacgatc ctatatgcac ttcctttcat
      181 tcaaaagaaa aatctatttg taattacaaa cagtttgatt gctttcaact atttaaagaa
      241 tcatacaagt tatcggattt tacttactgg tggcgaattt tctcctatta cagaagagtt
      301 cattggggaa gttgcagaaa gatcttttga aagtttgaat gtcgatatct ctttcgctgc
      361 aacaaacggc atatttgata acaatgtgac aaccgctcaa tttattgagg gaaatattca
      421 gaacgcagcg ctcaatgcag caaaaatcaa atgcattgtt gcagattatt caaaattaaa
      481 taaatcagac gtctatactt ttagaaaatt aaccgatttt gattatctaa taaccgataa
      541 tcatttatca gaacaggatt tcaatcatta ttcaaaatac ttaacaatat taaaggagcc
      601 caaaaatgat actgacaatc acgatgaacc catccattga ttccatttat tttacaaaac
      661 actttgaatt aggtacgatg acacgctttg attctccggt acgctatgta ggcggcaagg
      721 gaatcaattg cggacgagct tctgctctac taggttcatc agttatttta acagggtttt
      781 tgggcggtta ttttggcagt atgattcaag atatgctgaa aaatgaaaat ttgtttgatt
      841 tgcatttttt acctattgca gaagaatcaa gaggtgccgt cacgatcatg catgatgatg
      901 aaacgcagac agagctagtg gaagctggtc ctgaaatagc agatactact atcaaaaaat
      961 taatgaatga tatcaaacta atcagtaaaa ataattacct ttcagtcatt acaataaatg
     1021 gttctgttaa caatccagat ccctttttgt atgctaaatt attacaatat atcaaagaac
     1081 aaatagatcc ttccacctat atattaatgg atatatctgg acaacatctc cgtgaagtct
     1141 tagcaagtaa gtataaaccg atgttcatca aacctaatat ccatgagttt tcagatatca
     1201 tccatcaaaa aattactcaa aaaagcgaca taaaacacca tctaaaaaca aatccactat
     1261 ttaaagatat cgatgttatc atgatttctt gcggctcgta tggtgcagta gtcaaagtaa
     1321 aagaagaatt ttggaatgtt gagatcccta agattcctat cattaataca acaggctctg
     1381 gggatgccac tgttggaggt tttgctcatt cattagaaca gaattatcct ttaaaagaat
     1441 gtgtaaggta cgcaatggct tgcggcatgt cgaatgcaca gcaatcagaa gtcgggaaag
     1501 ttattccagc tgatgtaaaa aatttagcaa aaagcattaa aatcacagaa atgaaataaa
     1561 tagtacaaag taaataattg tttatatgaa atgagacaca aagagactgt gacaaaaaac
     1621 gtgtgtaccg acccccaaaa gttagatttt ttggtctaac ttttgggagt cgatacattg
     1681 tcacagtcct tttttcgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     1741 atattcaaca aacatgagaa gctatttttt gaaattgctc cttgtgactc agagttaaac
     1801 ggcaggctca cgacctcttg gtataaacgg tgttcaaaag cttgcatcta cgctaatgag
     1861 tccaaccaag tcaaaaatga ggagcttttt tggctgtcct tattacttga tgcagggcag
     1921 ttttttcata atctctttta gattaaatcc ttttggttac acaaggttct cttcaaagaa
     1981 tgttgccatt ttttcaaatg gaatgatgtc tgtctggtcg tatagatctg tatgggatgc
     2041 tcctggaatg atcatgagtt ccttgttgtc accttttagt tctttgaaag cgtcctcccc
     2101 aaagtaacgt gaatgagctt tttccccgtg aatgactaag actgcatgac ggatttctgg
     2161 aatataatgc aatagttttg cattcataag tgacaaccct gatgtcctat tccaaccatc
     2221 attggagttt aaagaacgga catgatatcc gcggtctgtt ttataatacg catgatagtc
     2281 tttgacaaat tgaggagcat cttcaggcaa cggatcaacc acacctccag cacgtgcgta
     2341 gtccccttgt ttgaaatcaa tgatgcgttg tgcatttaat tcttcacgca tttcatatcg
     2401 ggcattttca tccataacat cgaagtagcc atttgctgtt actcgagtca tgtcatacat
     2461 tgtggaaaca actgttgctt tgacccgcgt atcgatagct gctgcattca atgccatacc
     2521 tccccatcca caaataccaa tgattccgat tctctcaggg tttacatttt tttgtacaga
     2581 taaaaagtct actgccgctt ggaaatcttc cgtattgata tcaggcgaag caacatatcg
     2641 aggtactcct ccgctttctc ctgtaaacga tggatcaaac gcaatcgtta aaaagcctct
     2701 ttcagccatc gtctgcgcat atagaccaga cgattgttcc ttcactgcac caaatggccc
     2761 gctcacagcg attgccggca atttcttttc acttacttct tttggttcat ataaatcagc
     2821 gactaatgtg ataccaaaac gattcgggaa gctcaccttt cgatgattga cttgattgct
     2881 ttttggaaat actttgtccc attcttgtgt gatgtttaat tctttattca tattctagac
     2941 tctcctttga tcaaaattga catttgtttt tgaatgtatt attatgataa aacttaaact
     3001 tgactttagg tcaaggtaaa aattttattt taggaggatt tttttatgta ttccattgga
     3061 caagtgtctg aaatgtttgg attgcctgtt tctactttac gttattacga caaagaggga
     3121 ttatttcctg atatccagcg tgtttctggt attagaaaat tttcagagaa tgagttagag
     3181 gctttaagag tcattgactg tttgaaaaaa tctggtttag aaataaaaga cattaagcaa
     3241 ttcatggaat ggagcaaaga aggcagtaag acatttgagg ttcgtaaaga actatttgaa
     3301 aaacaaaaag aagtagtaga aaaagaaata gcaaaattag aaagagtact cgctatgcta
     3361 aactacaaaa gctggtacta tgaagaagca ataaaagccg gcaatgaaga agccgtattg
     3421 acaatgattc ctgatgattt accccaacat gtaaaagaag cttatgtaca ttctcattga
     3481 acaaacggat aactcactct ccttctctat gaacttcatg aaaacatgaa taaacacctc
     3541 taactgattg ttagaagtgt ttattttttc actcaagtat cctgcactta caaaaatatc
     3601 ctttttaccg ccatcctaat tctggagcga tatcttttac gattgtttca agcaagtgca
     3661 tgttatattc tacccccaaa gtattaggaa tcgtgacaag tactgtgtct gcttctttga
     3721 ttgcttcatc ttcagctagc tccttgatta attgatcagg ttcaccagca aaagtttttc
     3781 caaaaatcgt tgggtttcgg tgataagcaa gatagccgac agcatcttgt tgcggactac
     3841 cgtcttgtcc aaataagcgt cgatccaaat cagtggtgat tggctgaatc gagcgagtaa
     3901 ctaatgttct tggttcaaaa tcatgacctg cttgcttcca cgcttcttta tacgcacgta
     3961 attggtttgc ttgttggata tggaatggct cgtgggattc gtaattgact aatgtagatg
     4021 attgcagatt cattccatgt tgtgctgccc agattgccgt ttgctgcgaa ccagctcccc
     4081 accagattct ttgtcgtaat ccttctgaat aaggttctat ccgtaatttt cctgatcctt
     4141 gtgggaacat tggttgagga tttggttctt caaatggttt tccttcaagt aattccaaga
     4201 attcaagggt gcgttcccgt gttacctctt tgatttcttc ttcactgtaa tcataaccaa
     4261 agtatttcca gccatctaga acttgttcag gtgaaccacg ccccactcct agttgcactc
     4321 ttccttgcgt gatgatatct gtcaatccgg cattttctgc catataataa gggttttcat
     4381 atcgcatatc aataagtcct gtaccgattt caatcttact tgtctttgcg ccgattgccg
     4441 ctagcaaagg aaacggcgac ccaatctgag gggcaaaatg atgtacgcgg aaaaacgctc
     4501 cgtccactcc gattttttct gcttccaccg ctaaatcaat cgattgcaaa taagcatctt
     4561 gagctgattt cacaagcgat ccttgctcca tccaatgtcc aaagcttaaa aagccgattt
     4621 ttttcaaggt aatgacctcc tttaaaccta caaaaatgga aggtttttta tttttaatat
     4681 gaactgattc tactactttt tttcttaaaa gacgaataat tgttctcgcc tcacaatcaa
     4741 gaaatgtatc ggcttactac acccctactt tcttcatcat ctgccgtgtc ttaatctctt
     4801 ttaacagtct gttacatcgt tgatttttga ctcaaaatga ttgtttctaa catgtattta
     4861 tggtaaaaaa gacaagatta gttctttgta cacatcgatt tatacgctga acaaaaagaa
     4921 atatataaac taagaaaccc attgtttttt cgctccatca catagagttt actttagata
     4981 aacagtgaca tttactaatc cataacggag gaaatcaaat gaaaaaaatc tatttcttat
     5041 gtacgggcaa ttcatgccgc agtcaaatag cagaaggcta cgcgcgtaaa tacttgtctc
     5101 cttcaatgtt tgagataaga agtgcgggga tcgagacaca tggcttgaat ccaagagct
//
LOCUS       DAZUKA010000171         5102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000171 DAZUKA010000000
VERSION     DAZUKA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.175"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..948)
                     /locus_tag="ACG8PN_002623"
     CDS             complement(223..948)
                     /locus_tag="ACG8PN_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862735.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1102..1428)
                     /locus_tag="ACG8PN_002624"
     CDS             complement(1102..1428)
                     /locus_tag="ACG8PN_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862736.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1465..1830)
                     /locus_tag="ACG8PN_002625"
     CDS             complement(1465..1830)
                     /locus_tag="ACG8PN_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4862737.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1867..2082)
                     /locus_tag="ACG8PN_002626"
     CDS             complement(1867..2082)
                     /locus_tag="ACG8PN_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4862738.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2211..2546
                     /locus_tag="ACG8PN_002627"
     CDS             2211..2546
                     /locus_tag="ACG8PN_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4862739.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2622..4619)
                     /locus_tag="ACG8PN_002628"
     CDS             complement(2622..4619)
                     /locus_tag="ACG8PN_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862740.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4717..4992)
                     /locus_tag="ACG8PN_002629"
     CDS             complement(4717..4992)
                     /locus_tag="ACG8PN_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862741.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT         1601 a          998 c          656 g         1847 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatatc tttaccaact ttctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagt ttccaagtag gtggtatagc
      481 tgtgtatgtt tctacaaatg aattaagttt ttcattaagt gacttctctt ttatatttcc
      541 atctaaaaac tctgtatata tggcttgagc tatactaaaa caaagctggc cttgttctaa
      601 taattttaca tcattagtta gtaaagctgt gttcacgtta tcaatcgctt caagaagagt
      661 atctttggtg tccctaacag ctttaggtgt gccttctgga atttccctac ttgatacctc
      721 gtccatttta tcttccataa accccttaaa atcatatctt tctcccaaat ccatagaagt
      781 ttttgagaaa gctttcattt ctgagtctat ttcttcaata atatttaaca tgtctgatac
      841 atattgttta ggctgattcg tttttaatct atatgtggta aatatctcaa ttcctccaat
      901 tatcaataaa attgccaaac aaatccataa taattttttc tttttcattt ttcctcctat
      961 acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc agactattat
     1021 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac
     1081 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
     1141 atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc
     1201 taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc
     1261 acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga
     1321 cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac
     1381 aacgatacct ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc
     1441 tatctccaaa atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa
     1501 gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg
     1561 atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga
     1621 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
     1681 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
     1741 ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt
     1801 tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
     1861 tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact
     1921 taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1981 cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc
     2041 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt
     2101 tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc
     2161 attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga
     2221 agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat
     2281 tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
     2341 aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
     2401 ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta
     2461 gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg
     2521 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
     2581 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tataattggg
     2641 tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata
     2701 ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct
     2761 ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc
     2821 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa
     2881 gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt
     2941 tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
     3001 tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact
     3061 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
     3121 gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
     3181 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac
     3241 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
     3301 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
     3361 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
     3421 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
     3481 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
     3541 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
     3601 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
     3661 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
     3721 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
     3781 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
     3841 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
     3901 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
     3961 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
     4021 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
     4081 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
     4141 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
     4201 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
     4261 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
     4321 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
     4381 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
     4441 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
     4501 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
     4561 ttctctaata atttttcttt atctacataa attggaatat taatcattga tgtttccatt
     4621 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
     4681 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
     4741 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
     4801 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
     4861 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
     4921 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
     4981 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
     5041 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
     5101 at
//
LOCUS       DAZUKA010000172         4786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000172 DAZUKA010000000
VERSION     DAZUKA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.176"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8PN_002630"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8PN_002630"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4862742.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8PN_002631"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8PN_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862743.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8PN_002632"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8PN_002632"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4862744.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8PN_002633"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8PN_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862745.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8PN_002634"
     tRNA            3917..4000
                     /locus_tag="ACG8PN_002634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4786)
                     /locus_tag="ACG8PN_002635"
     CDS             complement(4240..>4786)
                     /locus_tag="ACG8PN_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862746.1"
                     /translation="IFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGN
                     KRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSI
                     FLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNE
                     FMQFGLTVLGICTFIITVINR"
BASE COUNT         1447 a         1001 c          772 g         1566 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaatt
//
LOCUS       DAZUKA010000173         4727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000173 DAZUKA010000000
VERSION     DAZUKA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.177"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8PN_002636"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8PN_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4862747.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8PN_002637"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8PN_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4862748.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8PN_002638"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8PN_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4862749.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8PN_002639"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8PN_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4862750.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1386 a          994 c          742 g         1605 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataat
//
LOCUS       DAZUKA010000174         4714 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000174 DAZUKA010000000
VERSION     DAZUKA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.178"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..316
                     /locus_tag="ACG8PN_002640"
     CDS             125..316
                     /locus_tag="ACG8PN_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862751.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(690..1502)
                     /locus_tag="ACG8PN_002641"
     CDS             complement(690..1502)
                     /locus_tag="ACG8PN_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862752.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1665..1772
                     /locus_tag="ACG8PN_002642"
     CDS             1665..1772
                     /locus_tag="ACG8PN_002642"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862753.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1830..2897
                     /locus_tag="ACG8PN_002643"
     CDS             1830..2897
                     /locus_tag="ACG8PN_002643"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4862754.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            3039..3242
                     /locus_tag="ACG8PN_002644"
     CDS             3039..3242
                     /locus_tag="ACG8PN_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4862755.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3302..4504
                     /locus_tag="ACG8PN_002645"
     CDS             3302..4504
                     /locus_tag="ACG8PN_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4862756.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1563 a          744 c          982 g         1425 t
ORIGIN      
        1 tgtagtcttt tctttattag gaatcatcac tgttgtatct tattaccttt tctgtttttc
       61 ttattagaga aaaataattt gagaggtaga tagtttttat tggaggaagt aattatgtat
      121 atttttgaaa caaaaatttt tcattgcaat aattgcaaca aagaaatttc atcgggggaa
      181 gaggtttgga caaaaatgga aatttccgcc aaaagtaact gctcctcaac tgaaatcaat
      241 taaagaacta gaatttgaga atgcactggt aatttgtatg acatgtactg atgaagtatt
      301 tcgaaaacat ttttaaaggt tgagatgtat atagatttaa ccaatcattt atctctctcg
      361 gaaaaggaat attaactaac cagcgggtta tcaaaaatag gtaggaaaac tgtacttaat
      421 ggcttatttc gcgctgatta tgagaatatc agtctgtttg attaattttt agggttcaaa
      481 atacagagac aaactccagg tcgggtttcg ccacaagcga attacccgat ctggagtttt
      541 gtctattgca ggaaggcgta gccggactgc aggaataaag cccccggttc taacaggggg
      601 ctaacaaaga acaggaaact gtgacaacta cccattgggg agtgtcctag cagtattttc
      661 tttgtcacgt gcttatcaat tttgtcacct cattttgatg atggatacgc tgcatttaac
      721 aggcgttctt tctcgacttc catcacgaat ggtagataaa gatattgctg ttctagtaga
      781 tattgattgt cctctgtgta ctgataagaa gcggtttctt ctgaatagac caacggggca
      841 attcgcacat cgctggtcaa ttgattaatt gagtatagaa ggatggcttt ctcacctagt
      901 tccttgggta aatgtttccc aaaataggaa taggattttt gatttgctaa atagacgagt
      961 atgcgatcca cactaaagtt ggagaaggta aagggcatca tgtgcagaat atgattttga
     1021 ttatcatgtt gcaaatagag ctgggcttcc ttaccaaaat tagttgaacc aagataaagc
     1081 tgcttttttt catcgataca ttggaaagta aactggtctt gttcagttgg tggaatgaga
     1141 tttgtaataa aaaattcaat cattcgatcc atttcttttt cactaacaag gtcagcgtgt
     1201 ttgatttcgt ctactatgag accttcaatc ttaggatcaa gcttacctgc ctttttttgg
     1261 agcatttcta aaatgtattg attctcttga tcaccataca aaagatattc attggtgaca
     1321 tgacctatct ctgcgatttg gtgaagggtt tcggtttttg gaaggttgat cccacgttcc
     1381 cagttattga ctgtacttcg cggtaaatca ccaattagtt tgccaaattt ctccatactc
     1441 aactttaatt cttggcgaat ctgccgaatt cgattgccga ctgcttgctt gtctatctcc
     1501 atgaaaactc ctcctcagga taagttctta aatttagtat acgtgtgtac ttaaaaaaag
     1561 tcaaaacata ttgacttaaa ataaatacag gcgtacaata aaaatgtact taaaataagt
     1621 tcaataggtt ctttgacaag taaatagcag aaggaggttt gtcgatgaac caagttaatc
     1681 aacatgatct cggtgaagcc attcgagttt ctcgagaaga aagggggtgg acacaacgat
     1741 atttagcaga aaaagtgggg atttcgagat agttattatc gaaagtagaa aaaggaacga
     1801 gacaattaag tgaagaaaaa ttgaatcttg tgttggatag tttgcaagaa gaggtcgttc
     1861 cagttaaccg tgttttgatt gattatctaa ccattcactt tttctcgaat caacatttaa
     1921 agctaattga agagattatt gaaatgccca ttgaacgttt tgaggaactg gattatgcgc
     1981 caaagggcta tattggccaa tacgtgtgga atcaagtaat cacgattcgg tattccattg
     2041 acgatgcggt taaaggaact gtgatggagt tttcaggtca aggctgtaaa cacttagcaa
     2101 gatggctaaa aacacgaaaa acaaattggc aacagttttt tcgtaaggta ttagattatc
     2161 aggggaactt tacacggatt gattttactt tagatgattt tgtggggagt ctcagcattc
     2221 cagaacttaa gcataaggta atactaggcc atgtgtggac cacttttcaa gtgagtgaat
     2281 ctcatggcgg tacggatatc ataaacaatg aatcaaatgg tgagaccttg tatttaggct
     2341 caaagaagag tcagtgtcgt ttttgctttt atcagaagga ccatgaacaa cggaagcgac
     2401 gaggaattcc tttagaagag gcaaaagtaa agaatcgctt tgaactacgg tatcgaaaag
     2461 agaaagcgca aagcttggca aagattattt caaaaaccca tgatttaacc aaactgttct
     2521 ttgagttact taatggagca atttgttttt atgaccgtga tccaaatgat ctaggtgcaa
     2581 aagttgatgc caaatgggca gcctttatta gcaattatgg cgcaatcacc atttctttag
     2641 atacaattcc tcaaagtttt gagaaaacta tgagttggtt aattcatggt gtttcaccat
     2701 cattagtttt tattgcacag gtcgatcaaa cgtttcattc ttccttgttg cctttgatta
     2761 ttgaacaagg agaaatgaat ccaagacaga tcaaacttct ccaaaatatg aaggcagacc
     2821 cggagtatta tcaggaggag gttgagtatt atgaacgaaa attacaaagt atatcaaatg
     2881 agaaaacaag ctactaaaaa agcaaagtac tgcttacaca acaagttgtg caacgtactc
     2941 cgcctcgaca agacgagtat aacacaacca tcgaatgaga aacaatagat tataaagagg
     3001 attattttaa aaggagtaaa gaatcaggag gtgagagaat ggaaacaaaa tgtgatttca
     3061 tggtaaatcg tgcaattttg atagagatgg gctttaaacc tagtcaagct gcacgaatga
     3121 ttaaagagag caaggcatat cttgcaagga ttgaaggtat tgatttttat aataatcgcc
     3181 aagttggtgt ggtgccatcc cgagtaattg aacatctttt tcacattcaa gttgcggaat
     3241 aaacagtaca agcgcggtac tattaaagta gcttttctca agtacaggga ggaatggaac
     3301 gatgccaaaa attaaaaata tttaccaaga taaaaagaca aaaaaatggt tcttccgagc
     3361 gtatctaggt atagatgaaa atggaagaaa ggttcagaag actaaacgag gttatgcttc
     3421 acaaaaagat gccaaaattg cttatgacaa gtacatggaa acacatgatt ttaacaagac
     3481 aattcctaat caactgtgtt ctaccaatca aatgactttc gaagagtttt acaaggatag
     3541 gtttgtaaaa tggtacgaga gacaggtcaa aagtcaaaca tatgagaatg cgcaattcat
     3601 tttcgaaaag cgtatgcagt ttttctatca ttttcgtgtt cgagatatta caagtcacga
     3661 tattgaggat tggttatttg aactaagcca gacagagtca cgtaattcac ggaaaaaagt
     3721 taatggcgaa acgttatcaa aatcttatat caatcggatt cgagggcacc taaaaattgt
     3781 attggatcgg gctgttaaag agggggtgat tgctaaaaat cccgttgaag atgtttcttc
     3841 gcttccttct gagaataaga aagttgagtt ttgggaacac agtgagtttc aaaaagtgat
     3901 gaatgaattg aaggatgata agattcaaac ccaccatcgg aagattgtgt atgaaatgct
     3961 cttttatact ggcattcgga ttggagaact tgaagctttg tcttggtcga atgtagattt
     4021 tgagaaaaac acgataacga ttgagaagac attgatttat aatacaaaag atgattggta
     4081 cttttcgact cccaaaacta aatgtgctta tcgaacgatt gggttgggga agaagctttc
     4141 cttaaaactc agacagtggt atgacttgca aaaacgtatt ggaaattttg aatatgttgc
     4201 tcagttggac ggaacattta cgccacctta ttcttttgcg aactggttaa aagaagctgc
     4261 taaaaaagcg ggtgtcaaac cgattaagct tcacgcgcta cgacattctc atgttgcatt
     4321 tctaattgag cagaatattc aaccgctatc tattcaagaa cgattgggcc atgcgaatat
     4381 tcaaatcact ttaggaactt acggtcactt atatgcaaag tcagatcaac aagtggtaag
     4441 tgcgattgat aatgttcaag agggcgctat tctttcaaaa aatgacaatt tattaccagt
     4501 ctgagttttc cacggttcta ccataagaca aataaatgct tttgcaccag tgttttcagt
     4561 cgaattgctc ctgttcaaga tgtatcggaa atagcgagaa aataagcaaa tttgcataac
     4621 tataataaaa aggacttagt tttgatggga aatttttcaa aattaagtcc ttttttatgt
     4681 ggttgaaacg ggtatgatca ttttaaggaa gtga
//
LOCUS       DAZUKA010000175         4620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000175 DAZUKA010000000
VERSION     DAZUKA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.179"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..2102
                     /locus_tag="ACG8PN_002646"
     CDS             162..2102
                     /locus_tag="ACG8PN_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4862757.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2189..4111
                     /locus_tag="ACG8PN_002647"
     CDS             2189..4111
                     /locus_tag="ACG8PN_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4862758.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4391..>4620)
                     /locus_tag="ACG8PN_002648"
     CDS             complement(4391..>4620)
                     /locus_tag="ACG8PN_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFS4862759.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1532 a          782 c          941 g         1365 t
ORIGIN      
        1 agattttcca atattatttt attcaacata ataaatcgta tatcaatttt tttagcgaaa
       61 tttctattta ttccaagaat ttcagttatt acggaatcat tttgtgctta ataaaaagaa
      121 accgttttta tatactataa atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa
      181 tcacatcctg tccagttggt attgcccata catacatggc agcagaaaac ttagagaaag
      241 cggggaaagc agttggcgcc gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa
      301 atgaattgac tgcaagagat atcgaagaag cagctggtgt aatcatcgca gcagatacta
      361 aaatagataa atcacgattc ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa
      421 tccaccatgc agatgagttg gtgagagata ttttagcagg aaaagcgcca gtctataaaa
      481 gtacggaggc aattatttct tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa
      541 tctataagag tttgatgaat ggcgtttcgt acatggtacc tttcgtagtt acaggagggt
      601 tattgatcgc gatttcttta acattaggag gaacagctac acctgaaggg atcaagatac
      661 cagaaggaac gatttgggca acgatgaata gtatcggaag tatcgctatg gggttgatgg
      721 ttccaattct atctgcattt attgcgcaaa gtattgcaga tcgtccagga cttgttcctg
      781 gatttgttgg tgggatgtta gcggcaaacg gagcattata tggtagtgat gcgaatgcgg
      841 gattcttagg cgggatcatc actggatttt tagcaggatt cattgcttta ggaatcaaga
      901 aagtgcgagt accgaaagct ttacaatcga tcatgccaat tattgttatc ccaattttag
      961 gatcattggc agttggtttt gtgtttatct atgttattgg agaaccagta gctcttctat
     1021 ttagttcatt gactcacttt ttagcaggga tgcaaggagg aagtgaaatt gttctagcta
     1081 tgatcttagg agcgatgatt gcttttgata tgggggggcc gttcaataaa gtagcctttt
     1141 tgttcggatc tggcttgatt gcagaaggca attattccat tatggggcca attgctgtag
     1201 ctatctgtat ccctccatta gcattggggc tggcatcatt gattttaaaa accaaattta
     1261 cgaaaacaga acgagaagct ggtaaggcgt ccttagcaat gggactgttt gggattacag
     1321 aaggcgctat tccgtttgct tcaacagatc cactacgggt gattccaagc attgtaatcg
     1381 gggcaatgac aggaagtgtg atcgcgatgc tttcaggagt gacagatcat gtagctcatg
     1441 gcggtccgat cgtagcggtt ttaggagcag tggatcacgt agccatgttt tttgtagcag
     1501 tgattgctgg tattgctgta accgtgctaa tgttaagaat ccttaaaccg acattacaac
     1561 aaactgcggt tgctgcagca aatggtgtga cagtagcaga tctatcagag actgaaacaa
     1621 gtgatgttta tacgaaaact gaaacttttg attctatcac ggatattatg tcagatgaag
     1681 caatcgtttt aggtgaaaca gcaacaacaa aagacggagt aatcgaagat ttagtccaac
     1741 gtttagaaaa aacagcggcg atcacagata aaaccagatt taaagaagtc atctatgata
     1801 gagaacatga aagtacaacg ggaatcggta tggggattgc tattccacat ggaaaatcag
     1861 cttatgttct taaaccaaca gtggcatttg ccagaagtca atctggggta gattggcatt
     1921 ctttagatgg aaaacctgca catatgatct ttatgattgc tgtacctgaa aacagccaag
     1981 gtgatatgca cttgaaaata ctgcaacgtc tatcacgtaa attgatggat gatgacttcc
     2041 gtcaagcact gatggaagcg ccagataaaa caacagttca ccaattatta agcgaaatgt
     2101 aacataagat gaaaaatcga ccgtgatgag acataataga tgtgttatca tgatcgattt
     2161 tttatttaga agggagtttc tgcacattat gaatcagcgt cagcaatctt tgatctatca
     2221 attaagtgag aacaataatt atataacaac aaaaaagcta gcagagcatt tccatgtgtc
     2281 agaaagaacg attcatagcg atttgaatac cattgaaact tggttgagag acaaacatct
     2341 gcctttaatg tttgaaagaa aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga
     2401 aagggaacaa ctgaaacgag cgcttaatga agagactcaa acagaaatgg ataaaaaaca
     2461 gcttcgtcag ttattgattt tccatctgct agtagcaaaa gacggattta gtcttgaaga
     2521 actttcagaa aaactatatg ttggaaaacg tacgattcgt aaagaattga cagagttgca
     2581 aagctttttt gagtatcacc atttacagct agtatccaaa acaaaactag gaacttttct
     2641 taaaggcgat gaacaagaaa aaagacagtt gcttgttaag acattacgaa atatgcagaa
     2701 agaagatcca caaagtccgt cgttgaaaga attcttcgct aaagatacat taaaagttat
     2761 ccaacataca ttacaagatg ttttttatga aaaccattta gaacagccga atggactggc
     2821 tagtgtggag attcacatct actttatgct cgagcgaatg aaacagtacc agaaagtgaa
     2881 attaagtaaa gacgaaaacg aagtcgtgga acacacccaa gcacaaaagt taagtagtca
     2941 aatattagca aaactagcca ccatctatcc aattgaattt tctcctgatg aaatcaatta
     3001 tctggctttg cgtatcgcca atctttttac aaattcacaa gcagccacac gttttcaaaa
     3061 ggaatctagc gcacttgctg atcatttgat cgaacaagtg gaacagtttc taggctattc
     3121 attaaaagaa gatcagctac taaaacaaaa tttaaggtct catttatctt caacttattt
     3181 tcgactccat tatggtttgc agatttctaa tccattgacg aaaaatgtgt ttagtacgta
     3241 cacacaactt ttcttagttt tacagttaat tttagaagat tattttgaaa aggaaaattt
     3301 ttatgttcca caagaggaga cagcttattt aacgatccat ttccaagcag cgatcgagcg
     3361 acacaaacat cagaaaagta gaagatatca aacggtttta gttagtgaat attcaaaagc
     3421 tatggctact tttctcgaag cacggctgaa cagagaactt ccagaattaa cgattatgga
     3481 tctagttgaa tataagccag aattaaagag acaagatttt ccctctgttg attttatctt
     3541 ggctacgtta ccatttaaac atataagcat tccagtaatc gagatctcac caatgatcac
     3601 agaaactgat ctagcctatt tgacaaaata tatgctggag catgtgccta tcaagaagaa
     3661 aaaaacattt gatctagcaa gctttactca tccatttctg atctttcctc aacttgaacg
     3721 gacagaccca gccgatattt taaactttat gggaaacgtc ttagtggaac atcattatgt
     3781 ggagccggaa ttcatcgatt ctgtcttgga aagagacagt cactccagta ctagagtagc
     3841 accatttgta acaatccctc acggtaatcc gctgtatgtg aagcattcga tgatttctat
     3901 tgctacaatg aaagaaccag tcctatggca cggggaacag atacgtatcg tactgctatt
     3961 agcaataaag aaaaatgatt tgaaacatgc agagttcaaa aaaatatttt ctgttattca
     4021 ctatctagaa aagcagccag aacaaatggc tcagttgatg caatccaatc atccgctaga
     4081 aatcttaacg ttgttatcta gttatgaata agtagcgatt aaaaaactgc ctttatattt
     4141 ataataattc tatctattta tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg
     4201 ccaaagttgt ggcacatcct tttattttga gtttcttata attttttata gttgcttacg
     4261 atgtcttgac tactggtaaa cggtgttcaa gagacaattt gtcggcaata aggcacaatt
     4321 ttgtaaaata tggaaagcta tttttttaac attgtttctt agtgcttgaa aaatcatttt
     4381 gaatacttct ttatacgatc gatgtacgtt gatttaagtc taaaactttt cttttgattc
     4441 cttctctgac ttttctgaat tccgttaata tttcttcttc gctcccgaca gcttgtgcag
     4501 gatcttttag gtcccagtga aggtgttcgt gattgtttgg aacggcaggg cacttgtctt
     4561 tggcatcacc acaaagtgta atgatcaagt ctgcctgagt aaagtaatct ctatcaatca
//
LOCUS       DAZUKA010000176         4576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000176 DAZUKA010000000
VERSION     DAZUKA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.180"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..898
                     /locus_tag="ACG8PN_002649"
     CDS             80..898
                     /locus_tag="ACG8PN_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862760.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            911..1198
                     /locus_tag="ACG8PN_002650"
     CDS             911..1198
                     /locus_tag="ACG8PN_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862761.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1380..1733)
                     /locus_tag="ACG8PN_002651"
     CDS             complement(1380..1733)
                     /locus_tag="ACG8PN_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862762.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1753..2808)
                     /locus_tag="ACG8PN_002652"
     CDS             complement(1753..2808)
                     /locus_tag="ACG8PN_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862763.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            3024..3422
                     /locus_tag="ACG8PN_002653"
     CDS             3024..3422
                     /locus_tag="ACG8PN_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862764.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            3425..3598
                     /locus_tag="ACG8PN_002654"
     CDS             3425..3598
                     /locus_tag="ACG8PN_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862765.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         1582 a          638 c          786 g         1570 t
ORIGIN      
        1 tttatgtata taaacatata cataaacgtg tttatagtgt ataatggagt tacaaaaggt
       61 taataaggga ggtttaatca tggaaaagaa gttgtcaatc attactggaa acattgaaaa
      121 aggtggaaca ggtaaaacaa caacaatcta caatctaggt ggatatgctg tcgaaaattt
      181 aggtatgaag gtgctgtata ttgatgaaga taagtcgcaa aacctatcaa aacgatttcc
      241 tgatgctttc agtaaagcga aaagagagaa tacaatcaat catttatatc ttcatggatc
      301 agctgaacct ttagctataa atgagaacat ggatatttta gtagcaggtg aagatttaaa
      361 acaagttgag atgcaaatgc gtgatatgcc taacaacaga accctactgt ttagatggat
      421 cgttgttaac tatgaacagc ttaaagagaa gtatgatcta attctgattg atactcataa
      481 tgatgcaggt ctactagctc aaaatgcttg ggctgtatct gatcttgtcg taggcatttc
      541 tgatccatct aaagatggtt ttgaagcttt gctgaagctt ggtaaagata ttgatgcact
      601 agcaaaacag ttgattgata ttaagactgg tgaaacactg atggaaacta aatacgctac
      661 gatcggtagc aggatcaaac ataatactaa ttcaagtcgt gagttcctag aattggtacc
      721 aacgttatct aattatcttg gatatgtaca agagaaggaa ttgcttaatg caactagctt
      781 agaaggtaag aatattgctg agttcgctaa ggataagaat atctatcgta agaataagga
      841 tttctttgaa agcacgttta cgctatacaa caaaataatt gatacggcac aagaataagg
      901 agtgattgaa gtgaaagaaa atagatttaa aaaattcgct gaagctgaag tagaagaagt
      961 tcctgaagtg gttgaagcag ttgaagaacc taaacaaaca cctaaagaaa caacaagttc
     1021 tttggtagct aaactaaaaa gtgatagttc tgaaaagaag aaaaattatc aattcacgct
     1081 tcaaccatca caaagaaaga aattatctaa aatcagcgaa aagttaggaa ttcgatcaga
     1141 ttcagcttta cttggtgaaa tgatagataa cttgtatgat gaagtttttg gtgagtgatc
     1201 agtttaaagc tgatcacttt ttttattgga ataagcggtt aggtatagct taactaaatt
     1261 tcgtatacgt gtttatggat atatacgtat acatgtttat atacatgtat acgtatatgt
     1321 ttatgtttgt atttgtacat aataaaaagg ctaggtattg taacctcgcc tttttatttt
     1381 taaagtaagt ctttgttact tggtgcattg aaccctttta gcattggctt ttctttcttt
     1441 gctgaatgat cggatgtaac cgttgaagga tgctcttgtt gaatagggtt atgatcagtt
     1501 ggtttcttct ttacttctgc tactttgcta tcgtttctat cgttgcctgc ttcaataatt
     1561 gtaacttcat tgttctttac acgccgatat aggttcaaag cttttttgat cgtctgagag
     1621 tatttaacgc ctagtgtttc atattcggat tcaagaaact ctgctaaagg taactcgcta
     1681 accttttctc tattgattat aactacataa cgctctgatt ttcccttatt catgttgatc
     1741 tccttcgctg ttttattgag ttaaaccaaa cttgtaaaag ccttttacat tagaaaattc
     1801 actgtcttca acaaacaagg tatgcggaat tttatctttt acaagttgtt tgtcgataat
     1861 agaagctcca ccaccagtaa agacaacttt atctaaggtt gtgaagtttt tgaatgtagc
     1921 taaaacttct gcaacaacca tattggtgta tgtttttatt tctctttcaa ctacttctga
     1981 aatatcaaaa ctttcatttc cgcttggctt aaatagatat acgccttttt ctttgccttc
     2041 tcggataatc ttttcaagtt ggaaagcatt aggactgatc ggaaggtcaa gcatatttac
     2101 gatacgatta tataaagtaa aaatgccggt atccttttgt tcacgattgt tttcgtcaat
     2161 ttcaaaattt tggacaacat caaaaagttt cgtgccacca ccaatatcaa caaaaccgat
     2221 cttctgactg atcaatgttt catcttgtac actaccatca tcagcaagta ggaggttata
     2281 tagtgttcca atcggctgtg gcagtaccca aacggtatct acgtttatat tataagcctt
     2341 accgtcaact tcaactgaat gttgtcgtgt ggcttgttgc atcacgtatt tgacgattgt
     2401 ttcggtatag tcggatgaag gcagacctaa aactagatta acggttaaaa cgtcatcaga
     2461 ttgatcttct aaacgtgcta ggttagctaa agagaaagat aggatatttt tgtaggtttc
     2521 ctgcttgtat ctgctgatcc caaaacctag tgattctatc acgtagtcat cttttcctag
     2581 ctcgtgtata tccttcccaa aataatagct ttcagtagtg tttactaggc tatattcaga
     2641 aacgtttagt gatcgtttta atgaggaacc tatattagaa aaacgtcctg aattaatgat
     2701 atttttgttc aaaatacgag agggaagtga gagtggagaa acgtgtgtac tcatttcttt
     2761 tactgattta ttccctaagt caacggaaaa caataatggt tctttcatgg tatgacctct
     2821 ttctgtttgt gtttatggta tttcggtacg aaaatagttt acacctattt actattagtt
     2881 gcaactgttt ataagaaaaa aatgcaatca taaaagtcta attaattata ataaagtgta
     2941 aataaaaagg tgtaaataaa tgcttgcaaa tttatcttat tatgatatta taaaatcata
     3001 aattatatta aggagtgatc ctgatgcgat tgagaagaaa taagaattac actaccatta
     3061 ttttagaaga ttttactgat caagaattac ttattgtcgg taaaacaaaa gcagatttta
     3121 tcgaaaaggg aaatgcttca atagtaaaag cttcaaaggt tgctgtcgaa aaatattttg
     3181 attctttaga agaaagtgct aaggaaattt ttaatggaga gaaagctggt agctatgagt
     3241 tagacggtat tagatttagc ttgatcccaa caactgaaaa tattattccg aagaaattat
     3301 attatatctt ttcatctgaa aaacatactt caatgaagcc ttgtattttt gattggcaat
     3361 ggttgattga tgctgtattg gatgtaaaat gttcaaaata caatattact ttggggtgtt
     3421 aattatgagt atgctagtta gaaaattacc attatctgta agagaatttc tattttactt
     3481 ggtctgctta atcgcatcag ggtttaagct gatcgaacat atttttatct ttgcttatgt
     3541 attagcaatg atcgcatttt tatctggcgg ttggttaatt atttatttgt taagctaagt
     3601 tttagatcaa aaataattag taaattaaac gataaaataa ccgttaataa atgtaatgaa
     3661 aggacaacgc ctaacaatca gcttgtaata agattataaa ataacataat atcaaaaata
     3721 gtgcttttta aaggacaaat taagtgtatc aacataaacg tgtatatgta tatgtttatg
     3781 ttgatacttt ttttgtctta attttttatt tttaaaattc tatttactaa tgctgtatag
     3841 atagtgatca gaaaaaacaa aatagcgaga ttaagctatt tattagttca tatattatat
     3901 tcagattaat atgatccagt attaatctga aggcaaaacc aatatatttc tacggactct
     3961 tgtaaaacta tttaaatttt cctgctaata aaataacgaa aatagaatat ttagcttttt
     4021 tataccattt tacgataaca tgatttcatt tgtaatatta aaacatttaa atttttaatt
     4081 attctttcaa attagatcaa ttaatatcct tataaaagag cataataaat ggtttttaca
     4141 tcgttattta ttgatattaa tctcatccaa aaaaaatgaa aacgcatact tttatgggtg
     4201 attgaatgtg attttaatac atataccaat taaaagaaaa ctttttagat ttttacttac
     4261 aaatttataa aaaaacaaga taattgatac tttgattttg cgaacatttt atcttgccgc
     4321 ctaattcagc tttaatatca tgattatatc agtaacaaat cgctttttta gctaatttaa
     4381 gcatcaaata attattctta atcagggaaa aaataactaa taaatggatt ttgggaatag
     4441 gtaaaaactt gatcggtttc tggctaaaaa taacgtgaaa aaggaaatat aaaataacat
     4501 ggagcgtggg aggaataaag aaattttaca cgaaaggtgc gcagggatgt aaagaaactt
     4561 aacacgaaac aaaagt
//
LOCUS       DAZUKA010000177         4572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000177 DAZUKA010000000
VERSION     DAZUKA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.181"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8PN_002655"
     CDS             complement(52..522)
                     /locus_tag="ACG8PN_002655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4862766.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8PN_002656"
     CDS             complement(681..1139)
                     /locus_tag="ACG8PN_002656"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4862767.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8PN_002657"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8PN_002657"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4862768.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8PN_002658"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8PN_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4862769.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8PN_002659"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8PN_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4862770.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1425 a          837 c          868 g         1442 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa tt
//
LOCUS       DAZUKA010000178         4453 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000178 DAZUKA010000000
VERSION     DAZUKA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.182"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..822
                     /locus_tag="ACG8PN_002660"
     CDS             28..822
                     /locus_tag="ACG8PN_002660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4862771.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            835..2601
                     /locus_tag="ACG8PN_002661"
     CDS             835..2601
                     /locus_tag="ACG8PN_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4862772.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            2624..4432
                     /gene="uidA"
                     /locus_tag="ACG8PN_002662"
     CDS             2624..4432
                     /gene="uidA"
                     /locus_tag="ACG8PN_002662"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4862773.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1639 a          534 c          898 g         1382 t
ORIGIN      
        1 aaacaaaaac ataaatagga gagaaatatg aataattgga tattttgtat tgattcagat
       61 ggctgtgtta tggatacgat gacttataaa cacaatctct ttttcggacc aattgctgca
      121 gagatttttg atgttcatca tagagaaaaa ttcctacagg attggaataa tattaattta
      181 tattcagaga ctcgtggaat aaacagattt ttaggtttag ttaaaacatt agaaaaaaat
      241 gatgtaacat cgattaatcg attaaagcac tgggtatctc taacaaaaga attgtctaat
      301 ccatctttga agagggaaat agaatattct cagtcagagg atttattaaa agctttaaaa
      361 tggtcggaag aagtcaatca acagattagt caagcaaaag gattagataa acccttttta
      421 agtgctagag atactataaa agctctgaaa aaatatggga agataattat tgtaagctca
      481 gctaataagg aagctgttca agaagaatgg gaaagacatg aattattatc attagttgat
      541 gaactgtgtt gtcaagataa aggaaaaaaa gaagatatta ttcgaagtgt aattgaaaat
      601 ggttgtgatt tggataagat attaatgatt ggtgattctc ctggtgatct agaagctgca
      661 aataaaaata aagttttctt ttatcctatt ttggtcaata aagaaaaaga atcatgggaa
      721 aacttaagaa ctgatgtttt atttaaatgt ttatcgggag attatgtaga tattgaacaa
      781 aaatatatag atactttttg gaaaaatctt actaataagt agaggagaaa gaaaatgact
      841 ataaaagtag atttacgaaa aaaaccatat aatttagatg aagatgctat ttcttggatt
      901 gaagagacga ttgattctat gactttggaa gaaaaaatag gtcaattgtt tgttaatatg
      961 ggatctagtc gtgataaaaa ttacttgagt aatatggtca atcagtacca tattggagct
     1021 gtacgatata atccaggaac tgctgatgag gtatatgatc aaaactatat cttacaaaca
     1081 gaaagtaaaa ttcctctatt aattgctgct aatacggaag caggtggtaa cggagcttgt
     1141 acagatggta ctgaagttgg tgtagaagta aaaattggtg caacaaatga tgcgaaatat
     1201 gcttatgaat tagggagagt ttctgggatt gaagcatcgg ctattggatg taactggagt
     1261 tttgctccta ttgtagatat taatagaaat tggagaaatc ctatcatttc tagtcgatct
     1321 tttggacaaa accctgataa agttttagaa cttagtttag cttatatgaa aggtattcag
     1381 gagagtggaa ttttgcctgc agctaaacat ttccctggtg acggtattga cgaacgagat
     1441 cagcatctat cttttagtat taatagttta ggtacggaag aatgggataa ttcttttgga
     1501 aaagtatact caggcttaat agaagctgga ttaccttcaa ttatggcagg tcatatccat
     1561 atgccagctt attcaaaaca ttttaatcct gatctgaaag atgaagattt acttcctgca
     1621 actttatcaa aagaattaat cactgactta cttagaggaa aactcggatt taatggatta
     1681 gtagttactg atgctagtca tatgttagga atgactgggg caatgaaacg atcagaatta
     1741 ttgcccactt ctattgcagc tggatgtgat ctatttctat tttttaatga tccagatgaa
     1801 gactttaatt atatgatgga aggctatcgt aaaggaatta tcactgatga tcggttatta
     1861 gatgccttaa aaagaatttt agggttaaaa gctaaattgg gattacacaa aaaagcaaaa
     1921 gaacaaattt tattacctaa gcaagaagct ctggaaaaaa ttggtttaat tgaaaataaa
     1981 tcaattttta aagaggtggc tgaccaagca attacattag ttaaagataa acaagatatt
     2041 tggcctattg taccagaaaa atataaaagg attttactag tagatgtaca tggggtaaaa
     2101 ggtgggtttg gtgaagtagt tgctggaaat agacctcaag ccattgattt attgaaacag
     2161 attttgtcag caaaaggatt tgaagttgaa aaatacattt cacctgaaga agaattgaaa
     2221 ggattatcgc aagaagaaaa aattaaaaat atagctggaa tatatgctgc taagagacca
     2281 attaaaaatt taactaaaaa atatgacttg atcattaata tagctaatgt taatccaggc
     2341 acagttcaac gaattgtctg gccagcaagt aaaggaactc cggatatacc gttttacatt
     2401 catgaaattc caacaatttt tgtatctgta cagtgtccgt ttcatttggc tgatgtacct
     2461 caagtaaaga cttatattaa tacttatgat ggaaaacaag aaactttaga aatattggtg
     2521 gacaaactaa tgggtctttc tgaatttaag ggagtaagtc caatagatgc ttattgtggt
     2581 ttaaaggata catttattta atatttagat aggagtggtt tatatgttgt atcccattat
     2641 gacagagacg aggcaagtaa ttgacttaag cggtctctgg agatttaaat tagatacaaa
     2701 tgatgactta caggaagaac ttgctttaga accaataaat gaaaaagaga gttatccgat
     2761 ttcagttccc tcttcttata atgatttatt tgaaacagag aacattagaa atcatgttgg
     2821 atgggtgtgg tatgaaaaag aagtgactct tcctaaaaga ttattagaag aaagattagt
     2881 tcttcgtttt ggatctgcaa cacatgaagc aaaagttttt ttgaatggaa agtttattac
     2941 tcaacacaaa ggtgggttta caccatttga gtctgaaatt aatcggtttg taaaagacgg
     3001 gaataatcga attacagttg ctgtaaacaa tattattgac gatagtactt tacctgtggg
     3061 gattatgaag gaaacagtac aggaaaatgg ggatataaaa aaggaaaact tagttaattt
     3121 tgatttcttc aattatgcag gtattcatag acctgttaag ttatatacaa ctccgaaaga
     3181 ctatataagt gatattacta ttaatacaag aataactgat ttggatgcaa gtgtagaatt
     3241 caatatcgat acagaatgga ccggaaaaat cttaatagcg atatatgacg aagaggctca
     3301 acttgtagct gaagctgaag gaaagaaagg aatgattcag cttaagaatc caattttttg
     3361 ggaacctcta gacgcttatt tatatagtat gaaaattagt ttaattgatg ataataaagt
     3421 gatcgatacc tatacagaag aatttggtat tcgcagtgtt caggttacca atggcaaatt
     3481 tttgattaat aataaacctt tttattttaa aggatttggt aagcatgagg attcgtatgt
     3541 taatggacgt ggtttgaatg aggctgtaaa tattaaagat tttaatttaa tgaaatggat
     3601 aggtgctaat tcttttagaa catcacatta tccttactca gaagaaatta tgcgattagc
     3661 agatcgagaa ggtattgtag ttattgatga agtacctgca gtagggttac atttaaattt
     3721 catggccact ttattagatg ataatgtgga aaaacataat acgtggaaag agatacgaac
     3781 agaaaattct cataaaacgg tgatcaagga attgatagaa cgcgacaaga accacccaag
     3841 tgttgtaatg tggtctattg caaatgaacc agattcggat tccgaaggtg ctaaagaata
     3901 ttttgaacct ttagttgaat taactaaaga acttgatccg cagaaaagac ctgtaacaat
     3961 cgttacttat ctaaaatcaa cgccagatgt ttgtaaggta ggagatattg tcgatgtttt
     4021 atgtttaaat cgttattatg gttggtatgt ttcgggaggg gatctggcac atgcaaaata
     4081 tctgttgaaa aaagagctag atggatggat ggaaagatgt cctgatacgc caataatgtt
     4141 tacagaatat ggagcagata cagtagctgg atttcatgat acagtaccaa caatgtttac
     4201 agaagagtat caagtggctt attacgaagc aaatcacgaa gtaatagata catgtgaaaa
     4261 ttttgttggt gaacaagtgt ggaattttgc agattttgca acaagtcaag ggattttaag
     4321 agtgcaagga aataagaaag gaatctttac tagagataga aaacctaaaa tgattgctta
     4381 ctatctacag aaacgatggt catcaatacc caatttcagt tataaaaaat aatttggatt
     4441 ttttaagaat gtt
//
LOCUS       DAZUKA010000179         4450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000179 DAZUKA010000000
VERSION     DAZUKA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.183"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACG8PN_002663"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACG8PN_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4862774.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACG8PN_002664"
     CDS             complement(2075..2977)
                     /locus_tag="ACG8PN_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4862775.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACG8PN_002665"
     CDS             complement(2974..3666)
                     /locus_tag="ACG8PN_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4862776.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACG8PN_002666"
     CDS             complement(3709..4350)
                     /locus_tag="ACG8PN_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4862777.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1363 a          952 c          771 g         1364 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc
//
LOCUS       DAZUKA010000180         4434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000180 DAZUKA010000000
VERSION     DAZUKA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.184"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1363
                     /locus_tag="ACG8PN_002667"
     CDS             29..1363
                     /locus_tag="ACG8PN_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4862778.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1404..2315
                     /locus_tag="ACG8PN_002668"
     CDS             1404..2315
                     /locus_tag="ACG8PN_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862779.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2312..3166
                     /locus_tag="ACG8PN_002669"
     CDS             2312..3166
                     /locus_tag="ACG8PN_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862780.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3163..3780
                     /locus_tag="ACG8PN_002670"
     CDS             3163..3780
                     /locus_tag="ACG8PN_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4862781.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3782..4300
                     /locus_tag="ACG8PN_002671"
     CDS             3782..4300
                     /locus_tag="ACG8PN_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4862782.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1327 a          755 c          900 g         1452 t
ORIGIN      
        1 cggttacatt aaaatttgga ggtaaaatat gaaaaaatta ttgtttacag ggttgctggc
       61 aagttcagca ttgtttttat taggaggatg cggatcaagt aatgcaagcg atgccagttc
      121 aagtgcagca aattcttctg aaaaaacaag tgatggaaga aaaaatattg atttctggtc
      181 cttttgggga tccggtgcac gaaaagaagc tatcgaagaa atcattgagg atttcaatca
      241 atcacaaaac gagatccatg taaattataa gtatcagcca tggggtgaca tctggactaa
      301 atctttatct gcaataacag ctggaaatgc acccgatgtt attgtacagg acataaattc
      361 cgttgcccaa agagcagaag cacagcaagc aactaatctc tccaaatatg tagaaaaaga
      421 cacttctaag gatttttatc cgcagctttg ggatacagta gaatatgaag gtgatcctta
      481 tgcgatccca ttcaatacag atacacaagt cattttttat aataaaaagt tatttaaaga
      541 agctggaatc agtgaacaag atctaccgac aacttgggat gaattagaag aaacagcacg
      601 taaactagat aaaaaagaag gaaaagaatt tacacgcatc ggtttttatc cattatggaa
      661 tttaggggct gatgtatggg cgctaaacgc agataacggg actagttggt ttgacaaaga
      721 tgaaaaagtc aagattgata ctaaaaataa agtggaagcc ttggagtgga tccaagactg
      781 gcaagattat tatgggaaaa atacgatcaa taaattagaa gccgaatttg ggtctggtgt
      841 atctgatcca tttatttccg gattagtagc catgcgtgcc caaaatatca attattactc
      901 tagtttgatg gaaaatgcac cggatgattt tgattttggc gtgatgcagc tcccagaaaa
      961 agaagaaggt tcaggccatt ggtcgtgggg tggcggattc gtcttggaag taccatacgg
     1021 agcaaaagat cctgaggcct cttatgaatt catcaaatat ctttcttctc cagaagtaca
     1081 agaaaaattc ggcgaaaaaa gttttgatat catggcgagc aagacagcca atgagaattt
     1141 agtgaataat gacaacctaa cggataaagg aaaaatgatc tatcaaatgg cggacgaaaa
     1201 ctttaaaaat acggtgatca caccagttcc tttagatgct ccggattaca cgacattagt
     1261 gaatgaacag attgaccaga ttcttttagg aacaaaatca ccaaaagaag gattggcaga
     1321 tgcacaaaaa gcagtagaaa acttagtcaa gcaaaatcaa taatccattt ttgactgtct
     1381 aggtacagag gaggcaagaa aatttgaagc gtttatctat ccgaaagaaa aaagaagcaa
     1441 gatgggccta tttatttatc tcgcctttta ttatcgggtt cttggtattt atgctagggc
     1501 ctatgctttt ttcagttatt ggtagtttca ccgattataa tttgacatcc aagatgaatt
     1561 tcattggtct agcaaatttc aaaagaatgt tcgttcacga tgatttattt tggaaatcgt
     1621 tatacaacac cgtttatttc gtcatcttca atgtaccgct gacgacgatt ttcagtgtgt
     1681 ttttagccac agtactcaat caaaaaatca aaggaatcag tgtattccgt acagctttct
     1741 atttaccggc agtcttatca ggagtagcag tctacatctt atggatgcag ctgctttctc
     1801 catctgcagg attgatcaac acatttctgg gctggttcca tatccctgga ccagcttggc
     1861 tgtttgatcc acaatggacg aaacctgcgt tgattttgat gaaaatatgg agttctggag
     1921 gagcaatgct actttactta gctacattac aaaatgttcc tagatcttta tatgaatcag
     1981 cagaaatcga tggagcaggc gtttggggaa agttcaaaag tattactttg ccattaatca
     2041 ctcctattat tttttacgat gtgatcacct ctacgatcgg ttctttccaa atttttcaag
     2101 aagcttatgt catgacgaaa aatggaacag gcggacctgc aaattctttg cttttttata
     2161 atctgcatat gtggaacaag gcgttcgtag cttttgatat gggttatgcg atggctatgt
     2221 caatgatcct gtttctgatc gtgttggttc tgacgtttgt caatttgaag cttgcaccta
     2281 agtgggtcta ttattctgga ggtgacggca aatgatggat tatttcaaaa agaccagtgt
     2341 gaatcggcaa acaaaaaaaa tactaagtta tgtcctcatg acagtaatag gaatcatttt
     2401 gatcattcca ttgctttgga tggtgtttac ttcattgaaa ccaatggaag aaatcgttcg
     2461 ttatcctccg acctttttcc cagaaaaaat cgtatgggaa aactatctgg atacgattgc
     2521 cgcctttcct ttttggcgct atgcaagaaa tacattattc atcactgtgc tagtagtgat
     2581 cggtaatgtc ctttcaaatt cttttattgc ttatggattt gcgaagcttg attttcctgg
     2641 gaaaaagctg atgtttgctt tggttctttc tacgatgatg atcccggggt ttgtaacgat
     2701 gatccctcaa tatgtattat tctccaagat cggctgggta ggtacttatt tgcccctgat
     2761 cgttccatcg ttcttcggca atgcgttcaa tatatttctg atgcgtcaat tttacttatc
     2821 gatcaataat gaattgatcg aagctgctga aatcgacgga gcgaatcatt tgtatatttg
     2881 gagtcatttg atgcttccat tgacgaaacc tgccttgatt accattgcga tcaattcctt
     2941 caacgcagct tggaatgatt tcttaggtcc tctgttatat atccaagatc aagaaaaata
     3001 cacgttacag attggcttgc aagtttttca gaaccaagca acgacacaat ggaattatct
     3061 gatggcagga gcaacacttg tcttgatccc gacgattttg ctgttctttt ttgcacaacg
     3121 ttatttcatt gaagggatgg atttgacagg aggttcaaaa ggatgatgaa tcgatcgatt
     3181 ggtaaaaaag ccaatgatta tttcttagaa atcattgaat ggattccacg tcttatccat
     3241 ttacagctac tatggttcct gtgcgtcttg cctgttttca cattaggaac tgcaagtcga
     3301 acagttctgt atatgattta tcattacaaa aaaaatgaag agaaaaagct cggttcacta
     3361 ttctggaaaa agtttcgtga gaacttctct ctttatggaa agcaagatag tttagcaagt
     3421 gtttattttc ttcttctatt gatcgattat cagattttcc gctattgggg tgggggaatt
     3481 ggctatacat tgatgtatac tacgttgttt ttgcttctat taagcgcgct tctctttagt
     3541 tacaaaatct tgcttcaact ggaacaaaaa gaagcttctt ggattactgc atttttcttg
     3601 ttttttaatg atttcaaaag cgcgcttttc tatttggcag gcacgttgat ccttcttgtc
     3661 gcattctggt ttgcaggacc aatctatttt gctttattag gtttcagttt cttgttttat
     3721 tttcaagtcc tgctttttat ccatcgtacg caagaaaagg ttcttaaaaa ggaggaataa
     3781 catgttgcgt ttgattgcta aaatcgatga tagtattatc aacaagttcg ccaagcaacg
     3841 gaaaacggat agtcgaaaaa tcaatttttt gaaatttatc agtaatatct ctaatggtgg
     3901 ttttatttgg ttgctccttg ctgctgtttt agtcttgata cctgatacta gagaatatgg
     3961 tctagccatt cttatcggca gtttgatcta tttttttgtt ggtttgcttt ttatcaaaaa
     4021 aacgacaaga cggaaaagac cttatgaaaa aaatcgtcgg cttgtccttt acacaagtaa
     4081 accgaagact tcttcttttc cttcaggaca tacagcgcta gcctttttat cagtagctgc
     4141 gttgttcgaa ttaagtccag ttgcaggaat attagccttt ttatttgctt gtttgatcgc
     4201 ttattcgagg atttttttac ttttacatta tcctactgac attttaggag gagctatttt
     4261 aggaagtgtt attggcatcc tatgcgctgc cgttatctga attaattgta cgaataaagc
     4321 atgagtgcgt aaatgcgcat ttgtgcttta tttttttgtt tcgaggaaag cgtagttgtt
     4381 tattcatttg tcttaataac ttaagttttg ttaaatgaga acgttataaa attt
//
LOCUS       DAZUKA010000181         4410 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000181 DAZUKA010000000
VERSION     DAZUKA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.185"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..344
                     /locus_tag="ACG8PN_002672"
     CDS             45..344
                     /locus_tag="ACG8PN_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862783.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            609..1484
                     /locus_tag="ACG8PN_002673"
     CDS             609..1484
                     /locus_tag="ACG8PN_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862784.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1481..1699
                     /locus_tag="ACG8PN_002674"
     CDS             1481..1699
                     /locus_tag="ACG8PN_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862785.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1686..4313
                     /locus_tag="ACG8PN_002675"
     CDS             1686..4313
                     /locus_tag="ACG8PN_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4862786.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
BASE COUNT         1701 a          551 c          902 g         1256 t
ORIGIN      
        1 ggtagaggag ttatagaata attaattaaa aaggagaggt ttttgtggaa gcaataaaat
       61 taatgatttc agaagaacag gaggaaatgt ttctaaagca gttttatcaa attgctacaa
      121 aagctattga aaaggctcaa gaaaatgcgg ggatgacaag ggaatatctc aatcaaaaag
      181 aaatggcgga atacttaaat gtgagtgaaa actttttaaa aaatgctgtg agaaatgagg
      241 gattgcccgt agttgaatta ggggcaagaa aattttattc gaaaaaggca gttaatgagt
      301 ttatgtttgc tcgaatgaaa agaggggaaa atattggaag atgatgaagt agaaattata
      361 aaaaaatgct ttaaaaaaag aaaaaaagtg ttcccaaaag tttgcagact gggggaacac
      421 tcaataataa agtgctgaga aaactttatt tatagctatt atatcaaaaa agaaggtgaa
      481 aaggtgtttt cttctggaaa tgttgtagtt gacaaactta gagaaataaa ttttgacgga
      541 aatgtaatcc acacacatgg tacgatcaat taagaaaaaa aacgaaaaaa ggtgttgaga
      601 agccctattt gaatgcaata gttatcttag cagaaattac ctattggtat agacctaaaa
      661 aaatttttaa cgatgatggt cagctacttg gttatcaaaa aaagtttctt gaagatattt
      721 tgcaaaaatc atataagcaa ttaagcaaaa aaacaggatt atcagaacga gctataaaag
      781 atgcaattgt gtacttagag aaaaagggga ttatcaaaag ggtttttaga aatatttatt
      841 atgagaaaag agcagttaca ttatcaaatg ttatgtacat tgttttaaat gtacataaat
      901 tgatagaaat tacttttcct actttggatt gcgaaaaaaa ggagtatatg ccgaaaattc
      961 gtcagagggg tatcgaaaaa aagagatatg tctctttaaa aaaaggtaga ggtatgtaca
     1021 aatttgtata tacgtataca aagactactt cacagaacat atcaaagact actctcgtta
     1081 tggaagaaga ggatattaat ataaagagga tttacagtaa gattagtgtt ctttatgacg
     1141 caaataacga agatggtcga aaattatttc aagtgtatca ctatttgaga aacgctaatg
     1201 tagatacaga agatatcaga aaaatcattg aatatttatc tgaaaatact cagtatctta
     1261 ttgctgagtg tattataaaa caacatgaag catgcttttt tcaaagaaaa actgaagaag
     1321 ggttatacga ttatgtaaca tattttttga atggacttca gaaaagatat aaaagtcaat
     1381 ttttagaaaa tgtagagcct atagatcagt tcaaaaaagc tttcgaaatc aaagagttac
     1441 ctgaaattac tatgcataat tggttgaaag gagaagaatc atgattaaaa atcgcttaat
     1501 agatcaaatg tggtgtcttg atcaaaatac agatactctc gaattaatcg agagtatctg
     1561 cttcaacaca gtagttctcg atttaagagc tgaaaatgac aattgtgtaa ctcatatcat
     1621 aattaatcaa aaaatggctc agcagctctc tgagagccta agaaaatggg ctgaaggaga
     1681 aaactatgaa agcaattaaa gaaaaaatcg ttagtagaaa aataaactgg ttagcactca
     1741 gtgtacttgt tttaatgatt agcgttgttt ttacaagtaa tattgttgaa gctagcattt
     1801 tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac
     1861 cttacctaaa aaaatacaat ggagatttcc ttagtcagtt cgggatttgg cttggatggg
     1921 ctgtggtaaa aggcatgttt actgtgactg attcgattca aaatatgatt ccagatgtgt
     1981 tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagtcataa
     2041 atacaattgt cgttggatta atgatccttt cattgatgtt tgttggctac aagatgatta
     2101 ctggaaaagg aacaatagat ttaagatctg ttggaatgaa tatagtgatg tctgtagcgt
     2161 taattctttt aatgccaaca atgatttcta gcggtattca atttgccaaa atattttaca
     2221 atgatgctac tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag
     2281 gagtaacaga tctggcatat attaataaaa cagatcaata ctcaagcatt gataaaacgg
     2341 aggatcgaaa taagttaaca cgaaaaaatt ttcaacagac agatttaaca caagtgttga
     2401 cggataaagt gattgataaa ttggaaaaag aaaatccagc tgcggataat cttcgctatg
     2461 aattagttga aaattctaat aatgaatttg ttgccacaaa gttttctgat aacttcttat
     2521 caacattttc tgatagcttg aaatcaggtt attatcgata tcaagcaaat ctttggggga
     2581 tttcgattgg gttgacagcg ttggcaatcg cctatgtgtt cagcgcgttt gtgatcatta
     2641 cagcaatatt agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcgacagata
     2701 ttgaaacagg acaacgttca aaagttgttt tatccgatat tttacaatgt tacttaacag
     2761 ttggatttca agggtttgga ctatctatgt ttgctatgtt tatcaatttt ttaaatgctg
     2821 gacaaggtat ctccacaaat atttttataa aaacaattgc ttatatctgt gctgtgtttg
     2881 tgctgataaa aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag
     2941 aaggctatgg gcaacttgct tctgcttttg gtatgggcgc aatgctgttt agaaaaggat
     3001 caactggatt taatcgtgct aaaggaagcg gaaatggaaa tggatcagac agtggagaag
     3061 gagaagatcg aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa
     3121 ctggaagagc tttgggatat gcccatgaac gtggattatc aggtttagct tcagatggag
     3181 ctacaatggc tagtgaaaga gcaataaaac catttaagag tatgcgtgat atggctaatg
     3241 ataccaagaa taagttcaaa gaaggtttag atgatggtac agtgtccgct attaataaaa
     3301 atagcaaacc tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca
     3361 tgccaacacg tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta
     3421 tgtctagttc tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc
     3481 ctgtaagtgc aattcagcaa aaagttcaac aagatattga agaacgtaaa aatgctatgc
     3541 acggtcaagc taagtctgct gaagaattaa ttaatcagaa gcgacaagaa gctaaataca
     3601 cgcctgaagc aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg cgtacaggag
     3661 ctaatacgga cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa
     3721 ataccggatt agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg
     3781 gccgatcagt agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga
     3841 atgttgatgt aagagagaat ttacaaagct cttcaaatgg tcaaggtact aagacaattg
     3901 atgttcgaga aaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa
     3961 gagagaatgt acaaaattct acaagtagtc aagggtttaa atcaatggat gtaagagaga
     4021 atgtacaaag ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc
     4081 aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa
     4141 agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg
     4201 aaaatgataa gggatcaaaa ccaagacgaa gatttaccta tgaagacaac gagttattca
     4261 gagatacact taatgatcct aatccgcttt tcgataatct attaaaaaaa taaaaggggg
     4321 aattgtcatg aaattaattc taaagattag tacgcatgat ttcttagaac ttgacgaatt
     4381 atgtgaatca gtaaagaacg caacaaatga
//
LOCUS       DAZUKA010000182         4401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000182 DAZUKA010000000
VERSION     DAZUKA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.186"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            267..995
                     /locus_tag="ACG8PN_002676"
     CDS             267..995
                     /locus_tag="ACG8PN_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4862787.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1140..3167
                     /locus_tag="ACG8PN_002677"
     CDS             1140..3167
                     /locus_tag="ACG8PN_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4862788.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3275..4372
                     /locus_tag="ACG8PN_002678"
     CDS             3275..4372
                     /locus_tag="ACG8PN_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4862789.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1545 a          714 c          913 g         1229 t
ORIGIN      
        1 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
       61 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
      121 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
      181 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
      241 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
      301 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
      361 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
      421 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
      481 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
      541 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
      601 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
      661 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
      721 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
      781 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
      841 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
      901 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
      961 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
     1021 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
     1081 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
     1141 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
     1201 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
     1261 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
     1321 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
     1381 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
     1441 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
     1501 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
     1561 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
     1621 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
     1681 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
     1741 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
     1801 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
     1861 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
     1921 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
     1981 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
     2041 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
     2101 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
     2161 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
     2221 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
     2281 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
     2341 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
     2401 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
     2461 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
     2521 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
     2581 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
     2641 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
     2701 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
     2761 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
     2821 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
     2881 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
     2941 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
     3001 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
     3061 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
     3121 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
     3181 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
     3241 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
     3301 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
     3361 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
     3421 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
     3481 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
     3541 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
     3601 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
     3661 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
     3721 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
     3781 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
     3841 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
     3901 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
     3961 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
     4021 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
     4081 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
     4141 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
     4201 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
     4261 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
     4321 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
     4381 taacaaatag taaattttta t
//
LOCUS       DAZUKA010000183         4385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000183 DAZUKA010000000
VERSION     DAZUKA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.187"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /locus_tag="ACG8PN_002679"
     CDS             complement(<1..355)
                     /locus_tag="ACG8PN_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862790.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACG8PN_002680"
     CDS             complement(615..1163)
                     /locus_tag="ACG8PN_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862791.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACG8PN_002681"
     CDS             complement(1164..2021)
                     /locus_tag="ACG8PN_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4862792.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACG8PN_002682"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACG8PN_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACG8PN_002683"
     CDS             complement(2583..2912)
                     /locus_tag="ACG8PN_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4862793.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACG8PN_002684"
     CDS             complement(2989..3261)
                     /locus_tag="ACG8PN_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4862794.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACG8PN_002685"
     CDS             complement(3261..3524)
                     /locus_tag="ACG8PN_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4862795.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            3758..4385
                     /locus_tag="ACG8PN_002686"
                     /pseudo
     CDS             3758..4385
                     /locus_tag="ACG8PN_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT         1328 a          873 c          586 g         1598 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3721 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
     3781 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
     3841 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagtaacg
     3901 cgatacagtg attattgctt gtgttatatg gggcataatt aatggctata ctagccaaag
     3961 agccacgtat agagcggttt gttccgtctt atttcctaat ggtgactttc ctagtaggag
     4021 tcgattcact cgcttaagtt caaacttagc ttacactatc aagattattc gatacttttt
     4081 catcaagaag ctcaccaaag gtgaactagt cggaattata gatagttttc ctagtccatt
     4141 atgtaaacca gttagaaata gacaagcaaa actattgaat caaatagcca aagttggcta
     4201 taactcaaca aaaaaatctt atttttatgg tcttaaaatt catatgatcg ttactaagac
     4261 tggctttccg attacttact caatcacgaa tccaggtgtc catgacgtga aagtgttaga
     4321 gactttatcg gaagaagcga atcttcccaa tatcctaggg gacaaaggat atattagcca
     4381 taaaa
//
LOCUS       DAZUKA010000184         4371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000184 DAZUKA010000000
VERSION     DAZUKA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.188"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1119)
                     /locus_tag="ACG8PN_002687"
     CDS             complement(100..1119)
                     /locus_tag="ACG8PN_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4862796.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="ACG8PN_002688"
     CDS             complement(1116..1340)
                     /locus_tag="ACG8PN_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4862797.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="ACG8PN_002689"
     CDS             complement(1337..1630)
                     /locus_tag="ACG8PN_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862798.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1667..1804)
                     /locus_tag="ACG8PN_002690"
     CDS             complement(1667..1804)
                     /locus_tag="ACG8PN_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4862799.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1806..2237)
                     /locus_tag="ACG8PN_002691"
     CDS             complement(1806..2237)
                     /locus_tag="ACG8PN_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862800.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2251..2745)
                     /locus_tag="ACG8PN_002692"
     CDS             complement(2251..2745)
                     /locus_tag="ACG8PN_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862801.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2745..3194)
                     /locus_tag="ACG8PN_002693"
     CDS             complement(2745..3194)
                     /locus_tag="ACG8PN_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862802.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3198..3818)
                     /locus_tag="ACG8PN_002694"
     CDS             complement(3198..3818)
                     /locus_tag="ACG8PN_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862803.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3818..>4371)
                     /locus_tag="ACG8PN_002695"
     CDS             complement(3818..>4371)
                     /locus_tag="ACG8PN_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS4862804.1"
                     /translation="KTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFER
                     WRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVRHAS
                     ISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEG
                     ALIIATVDDPKQNVFTLEKVGVI"
BASE COUNT         1172 a          955 c          690 g         1554 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgctc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataatgga acgaaaacca
     1741 ttacttgttc gttagtgaaa cagccccagt catacattag tttgatatct tcatatgaat
     1801 acatgttact tcccaccttg taattgtttt ttgatttctt cgatttcctt ggtattttgt
     1861 acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac tttctgactt ttctttcaat
     1921 aagacatttt cttgcttgat tgctacattg tttagcattg ctttagaatt tagctgtttc
     1981 aattcatcat tttctgcctt cagcgtttcg tataatgcct taatattagc caattcatct
     2041 acgctatcaa cagcattccc ttttttctct tcttcagtag ctaactcaac ccactgtttt
     2101 ttatcaaaat caaatcttgg tctccagtta ggaactggtg gtttaatctc tgtacagttt
     2161 tcagggatat tttcttgatt gttcaaaatg atttgctcga atccgtaagg cttgattgat
     2221 ttgtaaacta ctttcattag atttcctccc ttaaattgaa taagtgataa caaatgaata
     2281 agctgatcca taacttgaat ttcttctcca tttaatggct ccgtctgcgc caatagatag
     2341 ttgagcactg ttcaaagtag aacggtctat tgacccaacc agttgttcaa aactaattgg
     2401 tgtccgatag ccttctggaa cagttaaaat aaccgaatca ttcccgccac tactttttcc
     2461 gtttaaagcc acaaaatata tagaaactgt ttttccttca cgataaagct ttgctgtccc
     2521 tgtattaccg ttagtgacaa ccagatttac cacttcatat ctgttatcat cagtcgtcaa
     2581 aacacgttta ttattgattg ttacagaacc tgaaaaattc ttatgtcctg caatggcttg
     2641 gtctccatct gtctgcacca atgcttcttt accatcaata gcttctacat gcgtttttaa
     2701 gtatttagca actccgtctt cttttaattg aacaatatca gccattaaac cgtccccact
     2761 ttctcaaatg taatattcgc taatccatca agtttcactt tatcggttgc tgacattaaa
     2821 cctgctgtcg tagttgtagc attacctgga tttttctgtg ctccagctgc aattccatcc
     2881 aacttagttt tatctgtgga cgacatcaac ccatttgctg tagttgtagc tacagctgta
     2941 gttgtggcat ttattccagg atcaccttta tctccctttg gtaaaacaaa attaaatcta
     3001 gctgcagatg atgttcctac attcgtaaca gaagcggttg aaccactaga aacggttcct
     3061 atggtaattg ttgctgcttg gccaggatcg cctttatctc ccttcaccgt tgttggtttg
     3121 ccttctatag cattccaatg agtttgtgga taaacctgta cattgctttg ttttattttt
     3181 acgatatctg tcattctcta tacctcccca attttttcaa aagtaaaatt tggaattctt
     3241 tcgtttgtgt aattttctgc ttgatttaca gcttcttgga atttttgatc tacatatgac
     3301 tgattgacac caccagtccc actaccacct gtagaactaa ttgttccatc ttcatcaata
     3361 gtgatgtttt ctccagcagt ataatttttc agtgaatcga gtttttcttt taactccagc
     3421 gaaaaattga catcagattg tctgactgca gagactttcc ctgtctcatc aatcgtcaat
     3481 aattcaccaa cttttatgcc acccaaacgg tcgatagtgg caactggcaa cacgtagtca
     3541 ccttctccac tgtttaaaat tttttgatac atttctgccg tgataatgcc gtcatcttct
     3601 tcacttgcat agggtaattc tgtgagaaca ttctctaatc ccaaatcttc tttagtaatg
     3661 acgacggctc caatttttcc attaacagat aaaactgttg cttctcctgc tattatttct
     3721 tctaacccaa gaactgcgga agcatgtgtg attggaaaga attggcgttg tacacccgtt
     3781 ttttcatccg tttccatcat tcgtttagtt ttagccatta gatcacccca actttctcaa
     3841 gagtgaacac attttgcttt ggatcatcaa cggttgcgat gatcaaggct ccttcttcaa
     3901 tcgggaatgc tacagttcca actttttcga cttcatgatt atctgaaaag agatcatcct
     3961 gcaaaatatc agtcacttca atctccccgt attcaatcgt gaaaagcgtt gctcgcaatt
     4021 tctgatataa atagtccata tctgccagca aacgctctga aatagacgca tgacgaactc
     4081 cttgaatatc tactcgcgca tccgttagtt cggctaacat gactccacct ggatcaatcg
     4141 tctttaaaat atccttgatt gattcaaacc atgccaaata gtctgattct tgtccttctc
     4201 tccaatcttg gaaactgtgt tcctgttctt gtctccaacg ttcaaattct tctttccttt
     4261 cattcatcca gtccgtaaaa tcgcctttat tttcattaat aaagtccgtc atatccgcaa
     4321 ttaaatcttc gattgattgc caataagaac ccatttctcc ttctgttttt g
//
LOCUS       DAZUKA010000185         4333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000185 DAZUKA010000000
VERSION     DAZUKA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.189"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..887
                     /locus_tag="ACG8PN_002696"
     CDS             6..887
                     /locus_tag="ACG8PN_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4862805.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            900..2510
                     /locus_tag="ACG8PN_002697"
     CDS             900..2510
                     /locus_tag="ACG8PN_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862806.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2587..3246)
                     /locus_tag="ACG8PN_002698"
     CDS             complement(2587..3246)
                     /locus_tag="ACG8PN_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4862807.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3529..4194)
                     /locus_tag="ACG8PN_002699"
     CDS             complement(3529..4194)
                     /locus_tag="ACG8PN_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4862808.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1156 a          853 c          859 g         1465 t
ORIGIN      
        1 tttttatggc agaaatcgtc aaagtcacac atttgaaaaa gagctttggt cgtttcgaag
       61 ctttaaaaga tgtctctttc accgtacatt caggtgaagt cgttggattt atcggaccaa
      121 atggttctgg aaaatccacg acgattcgga cacttttagg gattataaaa aaagacggcg
      181 gaacggcaac gatttttggg caagatgtat gggaagatag tttatctatc catgaacgtc
      241 tttcttatgt tccaggcgat atctctttgt ggggaaattt gactggagga gcgatcatcg
      301 atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac
      361 gtttctctct tgatcctaag aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag
      421 tgggcttgat tgctgctttg gctgttgatt cggatctcta tttatttgat gagcctactt
      481 ctggtttaga tcctttgatg gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac
      541 agggcaaatc catcctactt tcttctcata ttctcagtga agtcgagcga ctagcggata
      601 aagtcgtgat catacgcgaa ggaacactcg atgaagaagg aacactcgat gaattgcgtc
      661 atttgactcg ttcgaccatc actatagaaa cagaagcaga gaatagtcac ttagcacaat
      721 tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg
      781 aagctttcga tgtgatttta gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta
      841 ttccaccgac tcttgaggat ttattcatga gacattatga aggatgagga ggaagcgaaa
      901 tgactgataa attttcacgc tggtccaccc ttttttctca aaaccttagc cgtgactgga
      961 aaaagatcct gatatggcta gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt
     1021 ttgaagaatt gacttccgga caaggattag ctggattata cgaaaccttg caaaacccgg
     1081 caatgattgc aatggtcgga ccaactccag cttcgaatgc tgctgagtat actttaggag
     1141 cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt tgctgctgtg atctctgttt
     1201 tacacgtggt gagccaaacg agaaaaaaag aagaacttgg tctgattgaa cttattcgtt
     1261 cctttcgagt cggacgtcaa gcaaactctt tagctgtcat cgctgaaagt attctgatca
     1321 atttgctttt agcaatatcg atcagctgta cgatgatttt tttccaagcg gattcgatca
     1381 ctgcagaagg tgcttggctt tttgggctat ctgtaggtag tatggggatt ttggggagtg
     1441 ccgttgcact gtttgctgca caactgatgc caagttcagc cagttcgaat ggtctgtctt
     1501 tagcgttttt aggtggactt taccttctac gagcaataac agatgtagct gattcctccc
     1561 tttcgatgtg gaatccttta ggctggtctt atttgaccta tccatttaca actaacaact
     1621 ggattccttt gctttatttg tttttgttca gtctaatctt gataatcagt tcatttgttt
     1681 tagaggaaca tcgagatttg aacaccggct atatccccga acgtgaagga aaaccgtatg
     1741 ctaaacgttc tcttttatcc ttgcgtggtt tattattcta tctaaataaa gggatgacaa
     1801 tcagttggct tatcactttt ggtttgatgg gggcggcata cggctctatc tacgggaata
     1861 tgcaacagtt tttagaaagt aacgacttaa tgaaacagat gtttatccaa tctggcgtct
     1921 caattgaaaa aaactttaca agcacgatca tggtagtctt gatttgtcta gctgctattt
     1981 taccaattgc tagcatcaat cgtttattta cagaagaaac ccacggacgt atgaatcagc
     2041 tgttcgcaac aaaaatttct aggaaccaac tttattggac caatatgtta ctagctattc
     2101 tatttgctat attggcaatc ctgattagtt ctggtgcact agggctttct gctttggctt
     2161 cgatgaagga tgcgaaagac atggatctat tgatgtttct taaagccggg ttcaatttcc
     2221 tgcctgccgt tttagtgttt atcggaatta gtggctttct attcggatgg cttcctcgaa
     2281 tcggtaaact tgcttatatc tacttagctt actcctttgc actcaattac tttggcggaa
     2341 ttttggattt accagaatgg ttttccaaaa cagctgtccc tagctgggtt ccacggatgc
     2401 caaacgattc ttttgatctt tccgtgttcc ttacgttatc atgtatcagc cttgcactgt
     2461 tcgtccttgg ttctattggt tataggcgaa gagacttact agaaggataa taaaaaagca
     2521 tgtacaattt catgctagcc tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt
     2581 ttttatttac aagtcttcta actgctcttt atcaatgatc cagacttctc gattctttct
     2641 ttttacataa ccattctcct ctaataattt aaattttcta gataaagttt cgggggtcgt
     2701 accgataaat gcagcaagtt ctttcatttt cattggcagg aaaaagtgat ccttccctgt
     2761 cgctttagct aagtcaagca gatagagagc gactcgttct tctacttttt ccatcatcaa
     2821 aaaagttgtc tgctgctgta cttttgttaa tttctcaacg ttgattgcta aaagtttcag
     2881 actaagggcg ggataatgca gtaagagttc cgagaaatct ttttgtctca atatgcaaat
     2941 ttccgttttt tctagtgctt cgccaaataa agactcattt ggaacgccta gcaactggct
     3001 ctctccctca tatccgcccg gctctattac tcgtaaaagc tgttcctttc cgctagcaga
     3061 taattgatac actttcatac tgccggaggc cacgatcact aattgaggtt ctccattcgg
     3121 cgcaagaatc tgttctcctt tttccacata gtgatgtttg acaagtgcgt gtactttttc
     3181 ttgatcttgc tgtactaagt gattgaacaa aggaacaaat gaaacacata aatgatcatg
     3241 actcataatc tctcctcctt ctaaacagct atttctaata aagaaacata acttccctta
     3301 ttccaactga taattttctt ttatcatagc ataaacgtca agcgaaccag ataccagcaa
     3361 atagagtgga tttctttttg tcccttgctt tattttcctt cttttgagac cttaagtaat
     3421 tgattcctat caatttttta aatatttact ttttgaataa tcaaaaacaa accttcactc
     3481 gcaagccgag agtaaggttt gtttttcgca ttgcttattt cgtgttgtct attgaaattt
     3541 ttccgcacct agcttttgat atagtggtgc tagttcttgt aaggtttcag cggttatttg
     3601 ctcaacgtgt tcgatttctt gcggatagaa gaatagttta cccatttgaa aggttcggtc
     3661 tctcatcgct tttttcaact cttcttgttt ctttgtatcg gacaaatagt cagacaaagc
     3721 taagtggtcg tcaagttcat gttctggttg cggccatatg taataatcct tgatatcgac
     3781 tgttttgctc cactctttca gtatcgcaag caactggtta tacgctgcaa tgctaccata
     3841 tcgttcttcg cttttataat gttggtacat caaatagatt tggtattgtt tttgattcaa
     3901 caaaactgct agacaggcat tctcgtcttg acgagattcg cttcggtacg cgctccaaaa
     3961 atgactgcgt aaattccagc cattcgtcca gctttcgatt ttaggtttgc tcatgccgta
     4021 catatcaggc aaaagtgcag ctgtctggag ctgaatctct ttccattttt gccagtgttc
     4081 tttatattct ttcttgattt gttcaatctt atctgttgga tggctctctt tgagctgttt
     4141 gaatgcgtag attggtcgat caaacaactg ataatctgat tttccaagtt ccattgtttc
     4201 gcctctttcc gttatctttt gtattctacc tgaattttgc gatatggaca aatagaggtt
     4261 gtgaaaaagc tgttctgcat caagtcataa gaacagccaa acaaaaaact gcgatgagca
     4321 tcaccgcagt ttt
//
LOCUS       DAZUKA010000186         4325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000186 DAZUKA010000000
VERSION     DAZUKA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.190"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1035
                     /locus_tag="ACG8PN_002700"
     CDS             112..1035
                     /locus_tag="ACG8PN_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4862809.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1041..2294
                     /locus_tag="ACG8PN_002701"
     CDS             1041..2294
                     /locus_tag="ACG8PN_002701"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4862810.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2428..3774
                     /gene="gor"
                     /locus_tag="ACG8PN_002702"
     CDS             2428..3774
                     /gene="gor"
                     /locus_tag="ACG8PN_002702"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4862811.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1490 a          683 c          936 g         1216 t
ORIGIN      
        1 ttattttgat tttttgtgaa ataattttca aaaatccgac tttaaataga agaagtattg
       61 gaaagttttt agagctatgc tactattatg aagttgtagg aggagaaaat tatgatcgaa
      121 ctcataacca cagtagaaac tattgaacaa gcagaacagt tgcttgctgc aggtgcagat
      181 acactatatt tcggtgaaga gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa
      241 gaacagcgac aattggtaga attagcacac aagtatggta aaaaagcgaa tattgctgta
      301 aatgggatca ttcatcctga aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa
      361 gaaataaacg tagatgtgat cacagttggt gatacaggta ttatttatac gatgaaaaaa
      421 aatcctgaat tgcgtatacc gtatatttat gatgctgaaa cattagtaac gagcgcacgt
      481 caaatcaatt tctgggcgaa aaaaggcgca gttggagcag tacttgctcg tgaagttcct
      541 tttgaagaga tgaaggcgat ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat
      601 ggtgctacat gcatccatca atccaaacgt ccactgctac agaattacta taactatacg
      661 aaacaagacg aacaaaaaga tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa
      721 gagacacatt attctattta tgaagatagt catggcacac atatttttgc cagcaatgat
      781 ctgaatctct cgaatgaatt gaatgaactt tatcagcatc attactgtac atggaaattg
      841 gacggattat ttactccagg cgaaaaattc gtaaacatca ctcgactatt cgccgaagca
      901 aaaaaacaaa ttttagaagg tagctgggat caagaaaaag cggaaaaaat gactagcgaa
      961 atcagtaaat tgcatcctga aaaccgtgaa ttagatacag gattctttta tattgatcct
     1021 tctgctatca aataaggagt atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac
     1081 cagaaatcct agcgccagca ggaacactgg aaaagttaaa aaccgcgatc cattatggag
     1141 cagatgcagt atatattggc gggaatgcat atggtttaag aagccgtgca ggtaatttta
     1201 cttatgaaga aatggctgaa ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg
     1261 ttgctgctaa tatggtcacc catgaaggaa atgaagatgg agcaggggaa tttttccgca
     1321 gtttgcgtga tgtcggtatt tctgcagtga ttgtttctga tccagctttg atcgaaattt
     1381 gtgcgacaga agccccaggt ctgccgatcc atttatccac tcaagcttca gcaactaatt
     1441 ttgagacatt ggaattctgg aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag
     1501 tttcaatgga ggaagtggca gagattcgta aaaatacgga taccgaaatc gaagcattta
     1561 tccatggtgc catgtgtatc tcttattctg gtcgatgcac attgtccaac catatgtcga
     1621 tgcgggatgc aaaccgtggc ggatgttctc aatcttgtcg ctggaagtat ggattgttcg
     1681 atatgccttt tggtcaagaa cgaaacagtt taactgatac aggcgaagtg gaagaagaat
     1741 tctcgatgag tgcagtggat atgtcaatga tcgaacatat tccagaattg attgaaaacg
     1801 gcgtagacag cttcaagatc gaaggaagaa tgaaatcgat ccattatgta tctacggtgt
     1861 caaatgtcta taaagcggct gtagacagtt atatggaaga tcctgaaaac tatgtctgca
     1921 agcaagaatg gatcgacgag ttatggaaag tagcacaacg agaattagct acaggttttt
     1981 actacaatac accaactgaa aacgaacaat tattcggtga acgtcgtaaa atcccagtat
     2041 ataaatttgt cggtgaagta ttatcttatg atgaggcaac acagattgct acgatacgtc
     2101 agagaaactt gttttctgta ggagatgaaa tcgaatttta tggtccagga ttcagtcatt
     2161 tcagtcaaac cgttgaagtt atgcataatg aagaaggcga atccattgac cgagcaccaa
     2221 atccaatgat gattttaact atgccagtcg cacagccggt gaaacctgga gatatgatcc
     2281 gaaaacgtaa ataaggaaaa agctgatata tccctgtttt aagcatcttc gcatacaaaa
     2341 ttcaagaaaa gtgttacact tgttttctgt agaaaaagga tcgttaaata aaggttttga
     2401 ttgatctctt caataaggag gaacaaaatg caaatatatg attacatcgt aattggtggc
     2461 ggctctggcg gtattgcttc agcaaacaga gcagggatgc atggcgcaaa agttttgctg
     2521 attgaagcga atgaattggg cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt
     2581 atgtggcaag caagtacgat tttggaaaca attgaacgag atgcctcaag ttacggtatc
     2641 caagcagatt taaaaaatgt tgatttcgcc ggtctagtag aaagacgaga aaaatatatt
     2701 gattttctac acggtgctta tcaaagagga ttagatagca atcatgtcga ggctataaaa
     2761 ggttacgcta cttttatcga tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct
     2821 cctaaaatct tgatcgctac tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa
     2881 tacgcaattg attcaaatgg attttttgaa ttaacagagt tacctaagag agcaatcgtt
     2941 ttaggtgctg gatacattgc ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa
     3001 gtcatgtggg catatcgaaa agaacgacca cttagaacgt ttgataaaat gctttcggac
     3061 aatttgatcc aaatgtacca agaagctgga ataaaaactt acgcacatca tattgctaaa
     3121 gaaattacta aaaacaatga cgaatatacg gtgctatttg aaaatggtga aaccttaact
     3181 ggtgattgtg tactttttgc cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa
     3241 aatacaaatg tggaattgga caaaaaaggg tttatcaaag tagataaata ccaaaataca
     3301 acggatgaac atatttttgc tgttggtgat gtgatcggta aattagatct gactcctgtt
     3361 gcaatcgcag ctggtagacg tttatcagaa cgtttattta atggaaagaa agattcttat
     3421 cttgactata aacttgttcc gacagtcgtc tttacgcatc caccaattgc aacaattggt
     3481 ttgacagaag aagaagcatt ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca
     3541 cgctttaccc caatgtactt tgcattaaat gattatcgcc aaaaatgcga aatgaaattg
     3601 atctgtgtcg gaaaagaaga aagaatcgtc ggcttacatg ctattggtat tggtgtcgat
     3661 gagatgctgc aaggttttgc cgtggctatc aagatgggtg caactaaaga agactttgac
     3721 aatacagtag ccatccatcc aacaggagcg gaagaattcg taacgatgcg ataatttaat
     3781 attaacagtt ccaacagctt tttattgaac aaaccatgta aaaccagaga atcagtatac
     3841 tgattttctg gttttttgtt ttttagttat ttatgtaaga ttgcttcgat aattctcaaa
     3901 acgaaataag tagggaagtt ggagattttg attatcttcg taactgacga agagtaaatg
     3961 aatccatttg gtatgcactc ttaagttcaa aatattggta tcgatgttta aaagaaatag
     4021 aaatgtatcg aagaacagat gtttaaaaaa gtgtgacttt gtataaataa attagaagaa
     4081 gcaaacgagg atgttaaaag aataccatat agttaccaaa ccaagaaagt tctcgtcaag
     4141 ttcgctggta cgaatcaaat caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt
     4201 tagtcacttt ttccgaacaa atagtgttca atcagctgtc cttcggtatt agctcaaaaa
     4261 acggcaaaat atgaggaact atttttccat ttgtttctct aatactcagg acaaaacact
     4321 gattt
//
LOCUS       DAZUKA010000187         4304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000187 DAZUKA010000000
VERSION     DAZUKA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.191"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..808
                     /locus_tag="ACG8PN_002703"
     CDS             107..808
                     /locus_tag="ACG8PN_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4862812.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1380..2396)
                     /gene="tsaD"
                     /locus_tag="ACG8PN_002704"
     CDS             complement(1380..2396)
                     /gene="tsaD"
                     /locus_tag="ACG8PN_002704"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4862813.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2393..2857)
                     /gene="rimI"
                     /locus_tag="ACG8PN_002705"
     CDS             complement(2393..2857)
                     /gene="rimI"
                     /locus_tag="ACG8PN_002705"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4862814.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2864..3406)
                     /gene="rimI"
                     /locus_tag="ACG8PN_002706"
     CDS             complement(2864..3406)
                     /gene="rimI"
                     /locus_tag="ACG8PN_002706"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4862815.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3390..4112)
                     /gene="tsaB"
                     /locus_tag="ACG8PN_002707"
     CDS             complement(3390..4112)
                     /gene="tsaB"
                     /locus_tag="ACG8PN_002707"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4862816.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1272 a          916 c          742 g         1374 t
ORIGIN      
        1 ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa
       61 aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa
      121 aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga
      181 agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct
      241 tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt
      301 agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct
      361 ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac
      421 tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc
      481 cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg
      541 tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt
      601 gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac
      661 cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa
      721 acaactacgt gattccggac tgttagatta tgacaaaaag cgttttactt tctgtaagac
      781 agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgatatt
      841 tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc agttttagat
      901 cactgggcaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat tcactgtttg
      961 caaaaaatac tgatagatta actagaggaa gcgtcttgtc ctcatccgac ctagattatt
     1021 cgatattcct cgacaataaa cgattaatca gtgagggcaa tcagaggaac ggataatgat
     1081 gaataagctg taaaaaatag ggctttgaca aaagcgtgtc acagccctta tttcgattaa
     1141 acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct
     1201 gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt
     1261 aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga
     1321 agccgttttt cctgggctgt tcttattact tgatgcaggg cagatttttc acaacctctt
     1381 tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt
     1441 tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga
     1501 gggggaatca ctagttctac ctcagggagt ttagttgcca aagcatcctg caatccttca
     1561 cgcaaccctt gattggccgc tacacctcca gctacaatca gttgcttgac tggataattt
     1621 tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct
     1681 gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata
     1741 aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgt
     1801 ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg
     1861 taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt
     1921 gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct
     1981 ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag
     2041 atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc
     2101 ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg
     2161 actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag
     2221 gtgatttgtt ctacgtgatg gcggctagct acttcaggaa ctacgccacc aaaacgttta
     2281 tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc
     2341 gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca
     2401 tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat
     2461 atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt
     2521 cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca
     2581 gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat
     2641 ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag atctggttat
     2701 cttcaactaa aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt
     2761 tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca
     2821 agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa
     2881 gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg
     2941 ttgcttgaaa acctaacttc cgataaagtc tttgtgcatc ttgattactc atacgcactt
     3001 ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca
     3061 gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat
     3121 ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta
     3181 gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca
     3241 attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca
     3301 attctcttag tacgtaacga cgatcgattt gttcgattgt tttttctgta taaatcattt
     3361 tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca
     3421 ggtgtgtgcg tctccaacca tttttcttct gcttctaccc gttttaaata gcgaggaaga
     3481 aaagcttgtt tctctgcaac tggttcagct aaagcaccta attgagccac agttacacca
     3541 cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt
     3601 tcatagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt
     3661 agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacaat ttctaatcta
     3721 ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc
     3781 acaataattt tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct
     3841 actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc
     3901 cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt
     3961 tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg
     4021 atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt
     4081 tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc
     4141 tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta
     4201 gtacaatgat acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa
     4261 aagctgcggt atcacttcat ttccccacag cttttccatc tttt
//
LOCUS       DAZUKA010000188         4295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000188 DAZUKA010000000
VERSION     DAZUKA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.192"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACG8PN_002708"
     CDS             complement(27..923)
                     /locus_tag="ACG8PN_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4862817.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACG8PN_002709"
     CDS             complement(916..1596)
                     /locus_tag="ACG8PN_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4862818.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACG8PN_002710"
     CDS             complement(1597..2379)
                     /locus_tag="ACG8PN_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4862819.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACG8PN_002711"
     CDS             complement(2372..3289)
                     /locus_tag="ACG8PN_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4862820.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACG8PN_002712"
     CDS             complement(3453..3707)
                     /locus_tag="ACG8PN_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862821.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1256 a          943 c          753 g         1343 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgt
//
LOCUS       DAZUKA010000189         4262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000189 DAZUKA010000000
VERSION     DAZUKA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.193"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..506
                     /locus_tag="ACG8PN_002713"
     CDS             54..506
                     /locus_tag="ACG8PN_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862822.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="ACG8PN_002714"
     CDS             520..1209
                     /locus_tag="ACG8PN_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862823.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="ACG8PN_002715"
     CDS             1334..3685
                     /locus_tag="ACG8PN_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4862824.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="ACG8PN_002716"
     CDS             3765..4160
                     /locus_tag="ACG8PN_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862825.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1505 a          534 c          861 g         1362 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tt
//
LOCUS       DAZUKA010000190         4067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000190 DAZUKA010000000
VERSION     DAZUKA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.194"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACG8PN_002717"
     CDS             complement(<1..1157)
                     /locus_tag="ACG8PN_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4862826.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACG8PN_002718"
     CDS             complement(1160..3409)
                     /locus_tag="ACG8PN_002718"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4862827.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACG8PN_002719"
     CDS             complement(3506..4009)
                     /locus_tag="ACG8PN_002719"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862828.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAZUKA010000191         4056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000191 DAZUKA010000000
VERSION     DAZUKA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.195"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG8PN_002720"
     CDS             85..1434
                     /locus_tag="ACG8PN_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4862829.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG8PN_002721"
     CDS             complement(1562..2152)
                     /locus_tag="ACG8PN_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4862830.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG8PN_002722"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG8PN_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4862831.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1168 a          742 c          788 g         1358 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagt
//
LOCUS       DAZUKA010000192         3995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000192 DAZUKA010000000
VERSION     DAZUKA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.196"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1348
                     /locus_tag="ACG8PN_002723"
     CDS             47..1348
                     /locus_tag="ACG8PN_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4862832.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACG8PN_002724"
     CDS             1375..1635
                     /locus_tag="ACG8PN_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4862833.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACG8PN_002725"
     CDS             complement(2634..3869)
                     /locus_tag="ACG8PN_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4862834.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1128 a          650 c          845 g         1372 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttcta
//
LOCUS       DAZUKA010000193         3990 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000193 DAZUKA010000000
VERSION     DAZUKA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.197"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1604)
                     /locus_tag="ACG8PN_002726"
     CDS             complement(99..1604)
                     /locus_tag="ACG8PN_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4862835.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1601..2287)
                     /locus_tag="ACG8PN_002727"
     CDS             complement(1601..2287)
                     /locus_tag="ACG8PN_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4862836.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2480..3901)
                     /gene="gndA"
                     /locus_tag="ACG8PN_002728"
     CDS             complement(2480..3901)
                     /gene="gndA"
                     /locus_tag="ACG8PN_002728"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4862837.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1096 a          849 c          686 g         1359 t
ORIGIN      
        1 ctagtttttt tataatgaca tccctgaatt tctcacttgc ttgtattttg taccttattc
       61 tttgttggat atataaaaaa acaagtaaag cacatgcttt acttgtcttc tgcctttgcg
      121 ataggaataa agattctaaa aattgtccct tgccctacta cactttctgc taagatacgt
      181 cctttatagt tttcaaccag ttgtttagcg atagacaacc ccagaccatt tccacccttc
      241 gttctagctc ttgctttatc cactcgataa aaacgattga agatcttatt aaggtcttct
      301 ttgggtattc cttcgccaaa gtcctgaatc gcgatctcaa attcattcag agtggaagaa
      361 atagagatat ggacttcttt tcgtgtagtt gaatacttta ctgcgttatc caatatgata
      421 atgattaatt gttcaaagtg attacgataa atttgcaggg tcacttcttt tgtcaaatca
      481 tcatctaaag tgatcacata gtctggatat aatatcttga aattattata aacttgatag
      541 gtgacttctt ttcctttagt tgtttcattc gcataatgaa catctacttg ttctgctctt
      601 gataagtcta gcatctcttg aactaagctt ttcatacggc tgatttcttg catgctggcg
      661 cttaatgatt cttctaatat ctcaggatca tctttccccc aacgatttag cagactaaga
      721 tgcccttcaa taattgcaac aggtgtcctt aactcatgag atacgtcttc cacgaattgc
      781 tcttgttgtt caatatacaa acgcatacgg tcaagcatct cattgaatat ttcagctaaa
      841 tctgccaatt catcatttgt gtcgatttca ggcatgtgga tatctgattg cggatcttta
      901 cgtatagtat ccattgtatc tctgagtacc ttgagtggtt ttaagaaata agaagataaa
      961 aagaaaccta aaatactact taagatcaaa gaaacaactt ctaaaacaat aagagtcaat
     1021 aatagcttat ttcgaatatc atagaaagaa gataattcat agaagctttg cgcatagccg
     1081 atcttttctc ttgtaccttt agaatatata ggctggatag ataaaaagcc agtttttcct
     1141 tctagtgtaa cgatcgttgg aactttacgt gtagtctgaa tcaggtttcg actgttttca
     1201 tgggttttaa aaatcaagtt ctcgtccaaa tcataaacat aaagatccat tgatggctgc
     1261 cctagctcag ctataaaggg atcgatcttc attaagtttc cttcgaccag tgaagtatga
     1321 tctacgacct catctccgtc atttgtcgct ttcgtcagat tacgatacgt atttactaat
     1381 gtcagttctt catccgagtt agccaaacga gaagctactt ctgaaatcgt acgctctaca
     1441 ttgtttcttt cttttgccac aatcagattg atagatgatt tataggtaat gacagcgaaa
     1501 attgtaaaca caacgaatat gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag
     1561 ggccctttca gttctctttt ttccgatttt ttcggcttta tcacgagcgc atcacataac
     1621 ctgttccgcg tacagtttgg atatagcttt cctccccggg aacgtcaatt ttgttgcgaa
     1681 gataacgaat atatacgtcc actacgtttg tttcaacttc tgtttcgtat ccccacactt
     1741 tatttagtaa aacgtcacgt gctaatacca cgttcacgtt ttccattaaa gtaagcagca
     1801 actcgtattc tctttttgtt agttcaatta cttcatttcc gcgacgtacg acacggtttt
     1861 ctttttcgat agtcaaatcg cgataagtaa tagtagtttg tttagccaca tttttatcgc
     1921 cttcaatatc gattcgacgc aacaatgcac gaagacgtgc taataattct tcgatagcaa
     1981 atggtttgac gatgtagtca tctgctccgt ggtcaagtcc agatacacga tcgatcacag
     2041 aatctcttgc agtcatcata atgattggtg tatttttgac ttggcgtaca cgtctgcaga
     2101 cttctaaacc gttcaattct gggagcatca gatccaacaa tatggcatcc cactcattat
     2161 ttaatgccgc atctaaacca gttcggccgt tgtagtggac ttctgcgtta tacccttcat
     2221 gcttcaactc tagctcgaca aatcgtgcca agttcttttc atcttcaatg attagaatat
     2281 tgctcattta ttattttatc ctttctattc tactggcttt ttaaaaactc tatcattata
     2341 ccgataatat gcttaaaaat ctatagtcat tttatcatgg atagcaattt taaaaaagag
     2401 ctgaagtcta ctcataacaa tcgacttcag ctctttcgtc ctctttttat tttctcattt
     2461 cctaacagcc gaaagactat tattcttcat ggtaccatga gtaatggaag attccttctt
     2521 tgtcgacacg ctcataggta tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg
     2581 caggcagacg ttctgctctg tatgaatcga agtaagcgat ggctgatgag aaagtcggta
     2641 caggtacgcc agcttggaca gccaaggcaa cgacgtctct gactgattgc tgatattttt
     2701 tcgtgatttc aacgaagtaa tcatctagca ataaattttc gatctcagga tttttgtcat
     2761 aggcatccgt aattttttgc aagaatcgag ctcggatgat acatccagcg cgccagattt
     2821 ttgcgatttc accaaatggc aagtcccaat caaattcttt tgaagcgaca cgtaattgag
     2881 caaatccttg ggcataactc atgattttac tgaaatacaa cgcttctcgg attttttcga
     2941 ccaattcttt tttgtctccc gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg
     3001 cttgtacgcg ttcttcttta taagcagaaa tatagcgggc aaatacagat tcagtgatca
     3061 atggaagtgg cacacctaag tcaagcgcgc tttggcttgt ccatttacct gttcctttat
     3121 tcccagctgc atcaaggatc acatctacta ctggtttacc tgttccttca tcgtctttac
     3181 gtgtcagaat gtcagcagtg atttctatca aatagctgtc taattcgcct tgattccatt
     3241 ctttaaagat atcagccatt tcttctactg aaaggtttaa aatattcttc attaagtcat
     3301 aagattcagc aatcagttgc atatctccat actcaatacc gttgtgtacc attttcacat
     3361 aatgtccggc tccatttggg ccaatatatg tgacacatgg tgctccgtct tctgcttttg
     3421 cagaaatttg ctctaagatt ggtgcaacta gcgcataggc ttctttttgt ccaccaggca
     3481 tgattgaagg tccttttaaa gctccttctt caccaccgga aacgcctgta ccgataaagt
     3541 tgatgccaga gttggctaac tcttcattac gacgcatcgt atctttaaag aatgtatttc
     3601 ctccgtcaat caaaatatcg cctttatcaa ggtgaggaag taactcttgg attgttgcat
     3661 ctgttgccgg tcctgctttg accattagca agatccgacg tggtttctcg atagattcta
     3721 caaattcttc gattgtatat gttgctttga aatttttatc tgggtgttct tctactacag
     3781 cggttgtttt agcccctgta cgattgaata aagccactga atagccgcgg ctttctatgt
     3841 tcaaggctag atttttaccc ataacggcca taccgacaac gccgatttgt tgttttgaca
     3901 tatgaactcc tcctacatac gggatttctc cctaatctct tacattatag cttaacacgg
     3961 ctacattgca aactaaagaa caacatattt
//
LOCUS       DAZUKA010000194         3987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000194 DAZUKA010000000
VERSION     DAZUKA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.198"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..467)
                     /locus_tag="ACG8PN_002729"
     CDS             complement(<1..467)
                     /locus_tag="ACG8PN_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862838.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCS"
     gene            complement(478..1146)
                     /locus_tag="ACG8PN_002730"
     CDS             complement(478..1146)
                     /locus_tag="ACG8PN_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4862839.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1276..1860)
                     /locus_tag="ACG8PN_002731"
     CDS             complement(1276..1860)
                     /locus_tag="ACG8PN_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4862840.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2191..2424)
                     /locus_tag="ACG8PN_002732"
     CDS             complement(2191..2424)
                     /locus_tag="ACG8PN_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4862841.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2439..3389)
                     /locus_tag="ACG8PN_002733"
     CDS             complement(2439..3389)
                     /locus_tag="ACG8PN_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4862842.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3386..>3987)
                     /locus_tag="ACG8PN_002734"
     CDS             complement(3386..>3987)
                     /locus_tag="ACG8PN_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4862843.1"
                     /translation="TFIKSNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHILF
                     GNILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMV
                     LLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIG
                     YSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT         1349 a          711 c          505 g         1422 t
ORIGIN      
        1 aaactacatc caatagaaat tttctgccgt tcaccacttg atagcgaaaa aatgttttta
       61 tctaatatat ttgaaagatc taataattca gctaattcat tgattctttt atccatatgc
      121 tcttttgtaa atccaaagtt ttccatcgaa aatactaact cactcgttgt atttgttgta
      181 aagaattgtc ctctaggatc ttgacttacg tagccaatat tttttgatat actttcttgt
      241 gttaaggatt gtagcgagtt attttcaaat aaaatttccc caaccaattt tccgtcataa
      301 agtcttggaa taagtccatt cattaacttt aatagtgtgg atttcccact cccagaagaa
      361 ccagtaatca atgtaagact gccttttttt attgaaaaat taaggtcaac aagcgcttgt
      421 tccttaacat ccttatatga aaaagaaaca ttgttaaatt caaacaattc tttcacctca
      481 aatcaaaata cttaagaaaa aaatagtaac ggaaaacagg atgatatagt ctaataagct
      541 catgtttgtt ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc
      601 attagcggtc atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa
      661 ttccaaatat tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc
      721 tttaatcatt cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact
      781 taacattatt tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt
      841 atagacacta accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata
      901 taaggatgtc gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat
      961 tactaaaaac agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa
     1021 catcaatact aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc
     1081 taaaaaaata atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt
     1141 tgacactctt tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc
     1201 accgttattc agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat
     1261 gcaagctttt tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa
     1321 tcttctggtt aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa
     1381 tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg
     1441 attgatcatt ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa
     1501 tgatgtaatt ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatcaa
     1561 atcgaatata cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt
     1621 ctctctccaa cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc
     1681 ttcattatac atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc
     1741 ttccttagaa gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaggcacag
     1801 ttgccttatc ttagtttgct gaaatccttc tttttcaaaa tggcttagtc cactttctat
     1861 taacttttgg tgagttataa tataatctct ggtttctatg ggtacttcct cctaataaga
     1921 tgaattaact ttattatctt acacaggact tgaagtttca atcgttaacg tttattactg
     1981 aactcattcc ttagagctaa actaatgaga aagagcagaa taactgatac aaaaagcaat
     2041 cctacaagaa ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag
     2101 atttaaaaaa attatatcca gctattagga taagtcctat cataatcact ccaatagcca
     2161 ttttcttatc ctccctaaaa ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat
     2221 aaattttttc cctttatcag ttaattgaaa tcctatatct aaatagtcct catatctaag
     2281 tttagccagc atctcactta cagatgctgg actaacactt agaacttttg ctaaatcatt
     2341 attttttaat ttcttttttc tttttgataa ttcctgaatt gctagtaaat aaattagtat
     2401 agtagtcgtt ttaatttgta ccatacctac cctccaaatt atttcgaaag gccttcagca
     2461 attttatcca ggttccattt catcatcgca tagtaactat caccatcttg tcctttttct
     2521 gcaattgaat cagtaaaaat cgttgaatag ataggaatat tggtatcttt tgatactgtt
     2581 ttcattggtc tatcatccac actgctttct acgaataagg agggaacttt tgttgtgcgt
     2641 aatttttcaa ctaagtgttt tatttgatct ggtgttcctt cttcttcagt gttgatttcc
     2701 caaatgtaag cagaaggcac attatacgct ttcgagaaat atttaaagca tccttcactt
     2761 gtaacaatca tttttttatc attcggaatt gaagcaaatt tagatttagc ttctttgtct
     2821 agagaatcca atttttcaat atacttatct agattttctt tatagaattt tttattatca
     2881 ggatcttttt ccgctaactg tttttcaata tttttagcgt aaataatacc gttttctaaa
     2941 tttaaccaag catgaggatc ttccttccct ttctcactct gaccctctaa gtaaataaca
     3001 tctatgccat cacttgctgc aaaatagtct ttgttttcct ctttgttcgc atttttaact
     3061 aattttgtaa accaagcatt tcctccagtt tccaagttaa caccgttata aaaaatcaaa
     3121 tctgcttttg aagttttttg aacatcttca ggcaaaggtt catattcgtg gggatctttc
     3181 ccaataggta cgatactgtg taaatcaatt ttatcttttg ctatattttc ggtaatatct
     3241 gctaaaatcg aattagtaac tactactttt aatttttcgt tcgaagccac tttatctttg
     3301 tctttactat ttattgaaca tgccccgaat ataaaaagag caatcaaaat ccccagactt
     3361 cccagtactg ctttccattt tctcatcata acgatctctc cttctttctt aaaaattttt
     3421 gttttggtga gattagaaaa ctaattacaa ataagaatgc cgctgttaaa acaatacttg
     3481 atcctgctgc tatgttaaaa ctataaccaa taaacaatcc aagcaatgat gaacttgctc
     3541 cgactgttgc agaaataaat atcatagttt tcaagctatt tgcatataaa tatgctgtag
     3601 ctgctggtgt aattaacatt gcaacaatta atatagttcc cacactttgc atagctgtta
     3661 ctgctactaa agttaataga accatcaata gataatgata aaaactgaca ttcatcccta
     3721 ttgcttttgc cattaatgga tcaaaagaag tgattaataa ttctttaaaa aatagaacta
     3781 ctataaccaa cactaaaacc gaaacgccaa ttgtaatcca cttatcaata tcttgtacag
     3841 ctaaaatatt tccaaataaa atatgaaata aatccgttga gctatttgca actcctataa
     3901 ggataactcc tagtgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta
     3961 ttacactatt gctttttata aaagtaa
//
LOCUS       DAZUKA010000195         3956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000195 DAZUKA010000000
VERSION     DAZUKA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.199"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            707..1747
                     /locus_tag="ACG8PN_002735"
     CDS             707..1747
                     /locus_tag="ACG8PN_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4862844.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2571..2903
                     /gene="traE"
                     /locus_tag="ACG8PN_002736"
     CDS             2571..2903
                     /gene="traE"
                     /locus_tag="ACG8PN_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4862845.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3522..3863
                     /locus_tag="ACG8PN_002737"
     CDS             3522..3863
                     /locus_tag="ACG8PN_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4862846.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1450 a          505 c          716 g         1285 t
ORIGIN      
        1 aactacctaa tttgggtagt tcttattttt tacattttaa gtagtattta attgcaattt
       61 ggtagtattt ttttattatc ttattcttag aacggtagta cttaatataa atataggtag
      121 tatctaattt aagtattaat ggtagtatct aaattaacag gtagtatgta actactttaa
      181 agtaattttt ttcagattaa cacagtagta tcttattaaa taagttaatt ttaatggtag
      241 tacataatta ttatatatat ctgacattgg tagtacttat tgtatttttt agtagtatat
      301 aatctattgt tttctagcct aaagccttgt cttataagaa taatataata tatactactg
      361 aaataaggta gtatatatta ctagtaatgg tagtatgttt tatatgcata aggtagttct
      421 taatttattt tttgttttga cactggtagt acttaattat ctttaatttt aaaactaaat
      481 tttattcata cgtttttctg gtagtattta attataaata atatttattt aaagtagttc
      541 ttaatgactt taaaggtagt acataattca ttttatttct gattttaggt agtatttaat
      601 taaaaaacag ttttaatgcg tttgcatttt ttatttatct ttgctatttt cgaaaagagt
      661 aggtaatttt acataaataa aacaacgtga aagtagagga tctacaatga ctgattttaa
      721 attttttaag gcggatagag tttacaacga attattttat caatttccaa aagtctttat
      781 tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag attgcctata tgcttttaaa
      841 agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat gaagaaggta atgtttattt
      901 tacttatacg acaaatgaac tatgtagagt attaaactgc caaaaacaaa aagcgatagc
      961 aatcaaaaaa gagttggaat cctttggttt attattacaa aagcagatgg gatttaacaa
     1021 acagttaggg aaaaataatc ctaatagact atatctagca gaattaaaag tctcagaaaa
     1081 tgatatctac ttactcgaaa aatttgatag agagaatagg gaaaacgttg ataaatcaga
     1141 gggtatgaaa atcataccca ccctcgacga aaaatcagac gctgaatccc ttggggctca
     1201 agagggtatg aaaatcatac cgtgccaaaa cgttgataaa tcagagggta tgaaaatcat
     1261 accagaactt aataataata tattagacac taatagacac aatatagaca ctgaaaaaga
     1321 ccgcctacaa gatcaattgt tgttagacaa ttttgagaca attatgacaa acgacagcat
     1381 tgctacgttt gtccctgaac gagtattaaa tttgataaaa acattttctt caagttacag
     1441 tgaagctcaa aaaaccgtcc agactattca taatgcaaag aaaaaagctg aaatagaaag
     1501 tgatatttcg atagtttttg aagaactcga tagttattat gtcaatgcag aacaagaatt
     1561 atacacgaca ctgttaaaag cctatcaaaa attaaaaacc gaaaaagtcg aaaatatcca
     1621 gaacctgatt tttgtctatg taaaaaattg gtttatcgaa aaaccaatag ctgctaaagt
     1681 atcaagtgaa aaacgtttga attatgcaag ctccccaagc actattacga aagactggtt
     1741 agagtgaaac tttactgaca aagtgattgt tgcttcatgg agtaacatta aaattaaaaa
     1801 ttttttatta ttctaattat attacgaata ttacaaaata atagctattt taatagaatt
     1861 tataagttaa aatattatga taaaaatcac aaacgtagcc ttttacgtca taagtgttaa
     1921 aagaaataat taatttcaaa tatagaaaag ggaacgtatg tttgcatact cctttttttt
     1981 gagttataat aatacgcgaa gagagataat ttcattattt aaacaagcta aaaatcttga
     2041 tattacttct tttcaggcaa tacgaatatt attcgcttac attttttgta taaatttaat
     2101 tgtctgctat gcttgtgatg aattgatttt cgttttgcac ttttggatga taaatttgga
     2161 caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt aaccttcgtc
     2221 agcgttcata cacggcatta agatttggcc cttaatgcgt taacctttca gtgttacggt
     2281 attacccgca atggatttag tataacagat attcaaaagt gataccactt tttttggctg
     2341 tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata atgttataaa
     2401 aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg
     2461 gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt tttgaaaaat
     2521 aacaagaaaa acttgttaaa aatatttaaa gaaattgggt ggtttatttt atgtcatata
     2581 caggaatttt gagtctcaaa gatatttgtc actacggtaa acggtgtacc gctacagaga
     2641 aaatcacgaa gaaactatca actggccaaa gtaaaaccgt tgtgcagtgt aagaaatata
     2701 taatccagaa agacaaagtc tcagaggaaa tgatttatta cattgggaaa caaaagcaaa
     2761 taattttaaa agatcctatc cccttaaaag agctatatcc aacaatcaaa catgtttatg
     2821 accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt accgcaaaag
     2881 ggatggggcg tttgatcggc tgaattgatc attctcataa cttgtatttt atcgtttttc
     2941 gcttgcttga aaatattgtc gagacttcta aaggaactta gagtcactag aaaaataagg
     3001 agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt ttaacgattg
     3061 gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt tggatagtgg tgaaatgaat
     3121 ttgtgtaaat aataagcgca gagtaaacaa gagggaattt agaagttaga caagttagaa
     3181 tagcgtctag attaatcatg agcagctact aagggatcta gactttgacg caaactcatg
     3241 gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg
     3301 cagtacatgt agatacttgc gaggacgttg attattggat caatcgagca tttaccgctg
     3361 tctactaggg tttctcgttt cggacgccat aaacgagtcg aaatttttta tgtattttta
     3421 agtaaacgaa aaacgataaa atatgaacaa gtcctgaata aagtgaaaaa attgatataa
     3481 aacatagaga aaatgaaatt tacgtaatgg aggaatgaca aatgaaaaat tatgatccga
     3541 atatacgatt gggcacacat actattaagg tttctttcca acgttgggat tacaaaggat
     3601 ttgtgacgtt tagaaggggc ggaaattgta aaggattaga tgttttagca ttagacgagg
     3661 atgatttata cgatcaaaca ttgactgata atccaattgg attcggcttg ctacctgaag
     3721 atgatgaagg taatgaatgg tttaaaatga cgcttatgaa tgataatgga gacgaattat
     3781 ctgttgaaga tacttggtca tatttgagtg attatattgt cagctttgaa atcattgaat
     3841 ttgtagcgga taaagaagaa taaaaactgg aggaataaat tttacagata aaggcttaga
     3901 aggagtcacg acgattaata aaaaggtagt attaccttag gcattgagta aggaat
//
LOCUS       DAZUKA010000196         3877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000196 DAZUKA010000000
VERSION     DAZUKA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.200"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACG8PN_002738"
     CDS             complement(717..3866)
                     /locus_tag="ACG8PN_002738"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS4862847.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAZUKA010000197         3849 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000197 DAZUKA010000000
VERSION     DAZUKA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.201"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..700
                     /locus_tag="ACG8PN_002739"
     CDS             14..700
                     /locus_tag="ACG8PN_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4862848.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            702..1646
                     /locus_tag="ACG8PN_002740"
     CDS             702..1646
                     /locus_tag="ACG8PN_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4862849.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1845..2489
                     /gene="pcp"
                     /locus_tag="ACG8PN_002741"
     CDS             1845..2489
                     /gene="pcp"
                     /locus_tag="ACG8PN_002741"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4862850.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2568..3845
                     /locus_tag="ACG8PN_002742"
     CDS             2568..3845
                     /locus_tag="ACG8PN_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862851.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1228 a          673 c          857 g         1091 t
ORIGIN      
        1 tggaggaaaa tatatggaaa attatcttag ccttttaggc gtcttgattg ttattctcgg
       61 gtttgcattt aaattagact cgatattgat cgtaatggtt gctttagttg ttacagcttt
      121 gactggcggt cttggcattc aaggaatgtt agaactttta ggtacaagtt ttgtaaacaa
      181 tcgcgggatg gcgattttta tcatcattat gttggcaaca ggaacgcttg aaagaaatgg
      241 tctgaaagaa tcagcggctt ccttgatcaa gcgattcaag aaagtatctg ctggtatgat
      301 tgtagatatt tatgggatct ttcgaatgat ctttgcggct tttaacgtga gttttggtgg
      361 agttgctgga tttgttcgtc caatcatttt accgatggca ttggggacca tcgaatcaaa
      421 agggttagaa gtccatccta agcatgaaga agaactaaaa ggtatggctt cagctatgga
      481 aaatgtctgc tggttctttg gccaagtact attcatcgga ggagcagggg cgttgctagt
      541 ccaatcaaca ctgaaagatc taggatatga cgtaacattg ggacagcttg ctttagtgga
      601 agttccagtg gctatcacgg ctttagttgt ggcaagtgtc tacttctatc ttaaagaaaa
      661 gaaattagcg aagaaatatt atccaggaaa ggggcaataa gatggacttt tttacaaatc
      721 ctgatatttt attaggcgat aaattactgg agatccttta tatcgtgatg ggactcgtat
      781 tgatttacac tggagtaaga aatttaacag ataaaaccaa tcctcatcgt tatgggtcag
      841 cctttttctg gacggtctta ggggtagtta ttgcaggagg aagatggctt ccggcaatcg
      901 tgaacggtgg cttgatcttt gcgatgacca taccagcaat tgctaagaaa gtcagcaaag
      961 gagaaagccg gcttccatca aaagaatata tggaaaaaat gtcagacaaa ctaggaatga
     1021 aaatttttat tccagctttg agtatcggtg tctttgcgat cttgtttgct ctatttacca
     1081 atttaggtgc tttagtcggc gtgggcgtcg gtgtttttgc agccatgcta atcttgatgt
     1141 tcttttctcg agataatcga ccaagtacat ttttagatga tgcagcagat atgctgggca
     1201 cagtagggcc gttaagcatg ttgccaatgc ttttagcttc tctaggtgcg gtatttacaa
     1261 gcgctggagt tgggacagtg atttccaatg cagtaggtac gatcgttcca gaaggaaatg
     1321 tgaacatcgg catcatcatt tacgctcttg gcatggtgat atttacggta atcatgggga
     1381 atgcatttgc ggccatcacc gtgatgacgg ttgggatcgg tgcaccattc gtatttagtc
     1441 atggagcgaa tccagcgtta ataggaatgg ttgcattgac ttgcggtttt tgtgggaccc
     1501 tgctgacacc gatggctgct aacttcaata tcgtgcctgt agcgatgctg gagatgaaag
     1561 acaaatacgg agtgatcaaa aaccagttgt ttattgcgtt atttatgcta gttttccaaa
     1621 ttgcttatat gattctattt aaataataga agagaggggt tgtgacagaa taattgtcgc
     1681 aacctcttct aacattgtag tggaacacta tttttttata agaatatatt aagtttgatc
     1741 tgttatgtta tttaaactat tgtttccttt atttacgctg ttcaaggaaa atggtatgat
     1801 aaaagataac catatgttaa atcaatcgga cggaggaaca acagatgaaa gtactagtaa
     1861 caggttttga tccatttggt ggggacaaag tgaatccagc ctatgaagca gtaaaaaaaa
     1921 tgccagatga aatcagcggt gctgaaatca tcaaagtgga agtaccgacc gtattcgaaa
     1981 aaagcagtca agtagtcaaa gaagccatcc aacagcacca accagatgtc gtgatctgtg
     2041 tcggacaagc aggtgggcgg tcagcagtct cgttcgaacg tgtcgcaatc aatctagcag
     2101 aagcaagaat ccctgataat gaaggaaatc agccattcga tacggcttta gaagaaaacg
     2161 gtccagccgc ttactttacc tcattgccaa tcaaagcaat gacaaaaaat gtacaagatc
     2221 atggtctacc agcgtacatc tcttatacag caggaacatt tgtctgcaat gatatcatgt
     2281 atcgtttgct tcatttgatc gaaacacaat tcccggcaat tcgaggtggg ttcatccacg
     2341 tgccattctc acccgatcaa gtgatcgatc gtccagtggg tacgccttct atgtctttag
     2401 aagacattgc ttcttctttg acttatgcag ttgaagcagc agtagaaaat aaagaagata
     2461 tttccggaaa tgccggaaca actcactaat ttagcaagaa acactggctg gttgctaacg
     2521 ttatgttagt agccagcttt ttgtaattag aaaggaaaga aacagccgtg gaagaatggc
     2581 aatccgtctt tgaagaatgg tttccaaaag aaatcagcaa aagctatccc atcaaaattt
     2641 ctaaacaata cacaagcagt caacgttggg agatctatgc aaaattaacg aaaaaacagc
     2701 gggaactagt cgataagcac cgccgatatc taatcagctc tcgttttatg gaagaacatt
     2761 atttagctgc aacagattgg gtgttttctg acttcaagat caatccgttt tttcgaacaa
     2821 aacgaagcca gcaaaaattg tattgtgagt gtggtagaga attaaaagtg caatacatcg
     2881 ttaaatcgcc aaaaaccgga aagatcctaa aactaggcat caaccacttt gccgatcacc
     2941 tgcatgtctc tccgacagtg gcagcttcga tccatcaggg aatgacaaaa gttgatctgg
     3001 ccttagacga attattatgg cttaagcaga agaatatcga ttttcctgaa ggactatggc
     3061 aaaaatactg ttttgtcttg taccaaaatc gtcggatgaa acagccatat ttaccggata
     3121 taaaattagc tcagcggctc gcagaatttc gccaagtaga aatgccgatt tacatagcgg
     3181 attatcaagc attggaaaat gaaatcaaga aaataagtga acacatcaat gggcagtcaa
     3241 aaaagcgaca gataaaaaaa gaactatttg acgactttgc agaagaatta gtaaaggatg
     3301 tagaagaatt cttgatcaac tatcgcgcat ttttacgtaa agattggcaa tccatcgtat
     3361 atgaagaagt gccagtacat cctaatgctt attttgaaac atttatttcc gtgttgagaa
     3421 agacaaaacg tcaacgaaca ccagaagtca ctgctcagat ggaatatttt gcgaaaaacc
     3481 aacgattcat ccaaccaaaa atctatctat ttatttggaa gcaatattgt cgctatggtt
     3541 ttacagaagg gttcttcgac agcattcctc ggatagtcag aaatggtttt ttaaaagtat
     3601 tgcgtaaaga acgagaagct atacaatctg ctgataagaa agaccgtaca gtctcaaaag
     3661 aaaaatggca actagtagtg aaagatatcc aatctggaaa tgtgcaagag acaatcgaca
     3721 aatggaaagg aaaacattat cgttttactg aagctcagaa acaagcgttg gaatattatc
     3781 agaagttaga agaatcactg cgtttcaatg atgaggcaag aaagtatctg aaggaattac
     3841 tataaaaaa
//
LOCUS       DAZUKA010000198         3717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000198 DAZUKA010000000
VERSION     DAZUKA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.203"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACG8PN_002743"
     CDS             72..155
                     /locus_tag="ACG8PN_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4862852.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACG8PN_002744"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACG8PN_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4862853.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACG8PN_002745"
     CDS             complement(1280..2143)
                     /locus_tag="ACG8PN_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4862854.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACG8PN_002746"
     CDS             complement(2145..2417)
                     /locus_tag="ACG8PN_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4862855.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACG8PN_002747"
     CDS             complement(2434..2643)
                     /locus_tag="ACG8PN_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4862856.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACG8PN_002748"
     CDS             complement(2721..3617)
                     /locus_tag="ACG8PN_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862857.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAZUKA010000199         3683 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000199 DAZUKA010000000
VERSION     DAZUKA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.204"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(234..773)
                     /locus_tag="ACG8PN_002749"
     CDS             complement(234..773)
                     /locus_tag="ACG8PN_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862858.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            complement(913..1593)
                     /locus_tag="ACG8PN_002750"
                     /pseudo
     CDS             complement(913..1593)
                     /locus_tag="ACG8PN_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1915..2136)
                     /locus_tag="ACG8PN_002751"
     CDS             complement(1915..2136)
                     /locus_tag="ACG8PN_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862859.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2234..2479)
                     /locus_tag="ACG8PN_002752"
     CDS             complement(2234..2479)
                     /locus_tag="ACG8PN_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862860.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2719..3333
                     /locus_tag="ACG8PN_002753"
                     /pseudo
     CDS             2719..3333
                     /locus_tag="ACG8PN_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1176 a          670 c          535 g         1302 t
ORIGIN      
        1 acactaaatt agtttatata attttccgta atatttaata tatatgaaaa ggaaaacgta
       61 gaaaattacg tctacgtttt cctatttagt tctatatttt cattaaattt tatatttgct
      121 cttttcactt ttcggaataa attcactgtg actcttgata ccttctttta tatattgcta
      181 atgtattttc tccattgcta tgaaaataga atcaacatat ttataatatc tatctaatct
      241 attttcattt caccgcagca tcttattaaa taacgtgtaa ataagaatat aaaagttatt
      301 gaactaatta ataaagatag aatcatcgta gttggatacg tagatgaaac ctcaactata
      361 gttcttttcc ccgtaccttt aatagacact aaaccattgt cattttctat agtgctcttt
      421 ttcccattta ctttcacaac tgttccccaa taattgaata caggtaatgt gatattttgc
      481 tcatcggcat cttctactgt atagactatt ttggaattat atgctttatg agatgcattc
      541 tcccaccgat caccaacttt tacttcatgt ttctttaaat gatctttata tttcaatgca
      601 tctaatggtg catagtcata taggcctcca gttcttgagg atgtataacg tgtaacatct
      661 tttttggtca taactaaacg acctttatac gactgaattt tatgcgaata atttgcttct
      721 gttgctgcag tcacagtaaa aataataaga actattccta caaggctcca catacttttt
      781 ttgttgcttg ttttccactt taaaaacact atggttaaga ttaatgaacc aaaaagcact
      841 tgaaaaccca aggttctcgg ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga
      901 actccaaata tcttaggctg ttattcccat taataccttg atttcagtag acaccgaaaa
      961 gccgaagagc gttccatttc ttcggttctt tttatatatt cctcgaatgg tctccatgcc
     1021 cttaatcgtg gaagaggctg tacggagact ttgataaaat ttatttcgtc gtttaatagg
     1081 tcgatggtct tgttctatta aattgttaag atacttcaca gttcggtgct ctgtcttagt
     1141 atataaaccc acactctgta actttctaaa ggcggagcca agagaaggtg ctttatcggt
     1201 cacaattgct ttcggctcac caaactgttt atggagtcgt tttaagaaag cataggctgc
     1261 ttgcgtatcc cgtttctttc gtaaccagat atctaaggtt aagccgtccg catcaattgc
     1321 acgataaaga taatgccaac gtcccttaat tttgatatag gtttcgtcca ttttccatga
     1381 atagaaggat tgtctatttt tcttcttcca aagataatag aggactttgc tgtactctta
     1441 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
     1501 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
     1561 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
     1621 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
     1681 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
     1741 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
     1801 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
     1861 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
     1921 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
     1981 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
     2041 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
     2101 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
     2161 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
     2221 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
     2281 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
     2341 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
     2401 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     2461 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     2521 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     2581 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     2641 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     2701 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     2761 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     2821 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     2881 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     2941 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     3001 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     3061 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     3121 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     3181 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     3241 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     3301 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     3361 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     3421 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     3481 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     3541 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     3601 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     3661 ttatacctct gctactaaaa aat
//
LOCUS       DAZUKA010000200         3682 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000200 DAZUKA010000000
VERSION     DAZUKA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.205"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..306)
                     /locus_tag="ACG8PN_002754"
     CDS             complement(142..306)
                     /locus_tag="ACG8PN_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862861.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="ACG8PN_002755"
     CDS             601..1929
                     /locus_tag="ACG8PN_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4862862.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="ACG8PN_002756"
     CDS             2131..2964
                     /locus_tag="ACG8PN_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4862863.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..>3682
                     /locus_tag="ACG8PN_002757"
     CDS             2980..>3682
                     /locus_tag="ACG8PN_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4862864.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQL"
BASE COUNT         1224 a          582 c          767 g         1109 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct gt
//
LOCUS       DAZUKA010000201         3680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000201 DAZUKA010000000
VERSION     DAZUKA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.206"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8PN_002758"
     CDS             <1..1087
                     /locus_tag="ACG8PN_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4862865.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACG8PN_002759"
     CDS             complement(1305..2447)
                     /locus_tag="ACG8PN_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4862866.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACG8PN_002760"
     CDS             complement(2452..2637)
                     /locus_tag="ACG8PN_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4862867.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACG8PN_002761"
     CDS             complement(2991..3236)
                     /locus_tag="ACG8PN_002761"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862868.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACG8PN_002762"
     CDS             complement(3230..3631)
                     /locus_tag="ACG8PN_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4862869.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1205 a          667 c          564 g         1244 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag
//
LOCUS       DAZUKA010000202         3568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000202 DAZUKA010000000
VERSION     DAZUKA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.207"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACG8PN_002763"
     CDS             complement(227..1114)
                     /locus_tag="ACG8PN_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862870.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACG8PN_002764"
     CDS             complement(1111..1980)
                     /locus_tag="ACG8PN_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4862871.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACG8PN_002765"
     CDS             complement(2117..3403)
                     /locus_tag="ACG8PN_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4862872.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1094 a          746 c          609 g         1119 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctaga
//
LOCUS       DAZUKA010000203         3555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000203 DAZUKA010000000
VERSION     DAZUKA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.208"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            515..2026
                     /gene="gadC"
                     /locus_tag="ACG8PN_002766"
     CDS             515..2026
                     /gene="gadC"
                     /locus_tag="ACG8PN_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFS4862873.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2039..3439
                     /locus_tag="ACG8PN_002767"
     CDS             2039..3439
                     /locus_tag="ACG8PN_002767"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4862874.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
BASE COUNT         1098 a          578 c          698 g         1181 t
ORIGIN      
        1 cattcaaatc gcctttgttc gctagagtct ttagaatttc tgtagtaaaa tcgttcatat
       61 ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag ggacttcctt
      121 ttttctattt acacaaaata ttttacactc tcaacaaaag tatcttcgat taatacttgc
      181 tcaagttatg tgcagctacg aaaaagtagg taccgaggta ctttcttttt cgcagctttt
      241 tttggactta tgagaagtac attcgctgga agaatacgat ataaaacgag ctttttcact
      301 aaagatatta atagagtgaa agaaacaagc ttttgttgtc agttttttca cttttctaaa
      361 aatgaaaata aaaaattctg gaagtggaga agaattttgc ttgctatact ttgtcttgta
      421 gaaaagaggt agacaaaata atgcaagctc aaataagcga ttttccataa ccttggtagg
      481 aatgacaaat taaataggaa agggaagtaa taatatgaat aaaaaaaagt tatctctatt
      541 cggttttttt gccctgacag cttccatggt tttgactgtt tatgagtatc cgacctttgc
      601 aacatcacaa ttacatttgg ttttctttct tcttttggga ggtattttat ggtttctacc
      661 agttgcttta tgtgctgcag aaatggcaac agttgaagga tggaaagatg gtgggatttt
      721 cagttgggtt agtcaaactt taggagaacg ttttggtttt gcagctattt ttttccaatg
      781 gtttcaaatt acagtaggtt ttattacaat gatctacttc atactaggag cgctgtcgta
      841 tgtaattaat tttccagagc ttaatacaga tccatttatc aagtttattg gtgtattagt
      901 cattttctgg gtactgactt tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc
      961 taaagcagga tttgtcattg gaattgttat tccttcgatc attctctttg gtttagcagc
     1021 agcctacttt atcggcggta atccgattca aatggctatt aatgcgaaag cttttattcc
     1081 tgacttttct cagctttcaa ctttagtagt atttgtatct tttatattgg cttatatggg
     1141 cgtagaagct tcagcatcac atattaatga gctggaaaat ccccaaagaa actacccatt
     1201 ggcaatgttt ttactagtga ttttggccat cgccttagat gcaatcggcg gtttctcagt
     1261 agcagcggtt attccacaac atgacttgtc tctcagtgct ggtgtaatcc aaacatttga
     1321 aacgcttatt ttacatttta acagtcattt acattggttg gtaaaagtga ttgctctcat
     1381 gattgcctgt ggcgttatgg gtgaagttag ttcttgggta gttggtccat cacgagggat
     1441 gtttgcagct gctcagcgag gtctgttacc agttttcttc cggaaaacta ataaacaaga
     1501 cgttccagtt ccgttgatta tgatacaagg aattatcgtt actatttggg cagcagtctt
     1561 aacttttggt ggaggaggca acaatttatc attcttagta gcaatctcct taactgttgt
     1621 catttatctc gtaggatatt tactattttt cctaggttac tttgtattag tttttaagaa
     1681 aaaagactta aaacgaactt acaatgtacc aggaaaaact gttggcaaaa cgattatcgg
     1741 tggaattggt tttattttat cagtgttcgc attgcttatc tcttttgtac caccagcttc
     1801 aattgcagct agtgagcacc gaacatacca aatcatacta cttattagct ttgtagtgac
     1861 tgttctttta ccttttatca tttatgaagt acatgataaa agtgctcaca caactattga
     1921 ggaacctact cacgtgaaag ctgaagacgt taatcccgct gtttatccag ctgctagagg
     1981 agagcaccac gttacaaaaa atgatgaaca tattttaaaa cattaggagg aattcattat
     2041 gttatacgga aaagataatc aagaagaaaa aaactatttg gaaccaattt ttggctctgc
     2101 aagtgaggat gttgacttgc ctaaatataa gttaaacaaa gaatccattg aaccacgaat
     2161 tgcttatcaa ttagtacaag acgagatgtt ggatgaagga aatgcgcgat taaacttagc
     2221 tactttttgt caaacgtata tggaacctga agcagtgaaa ttgatgaccc aaacgttaga
     2281 aaaaaatgca attgataaat cagaataccc acgaacaacg gaaattgaaa accgctgtgt
     2341 aaatatgatt gctgatttat ggcatgctcc aaataatgaa aaattcatgg gaacttcaac
     2401 gatcggctct tcagaagcct gcatgctggg tggtatggcc atgaaatttg cttggcgtaa
     2461 acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa cctaacctgg tgatctcttc
     2521 tggttaccaa gtttgttggg aaaaattctg tgtatattgg gatgtggaac tgagagaagt
     2581 cccaatggat gaaaaacata tgtcaattaa tctagatact gtcatggatt atgtggatga
     2641 gtacacaatt ggtattgtag gtattatggg tattacttac actggtcgtt atgatgatat
     2701 caagggtctg aatgatttag ttgaagctca caataaacaa actgactata aagtatacat
     2761 tcatgttgac gctgcatcgg gtggctttta tgcaccattt actgaacctg atctagtttg
     2821 ggattttcaa ttgaaaaatg ttatctcaat taattcttca ggtcacaaat atggtttggt
     2881 atatccaggt gtgggttggg tcttatggcg tgaccaacaa tacttaccag aagaattagt
     2941 atttaaagtt agttacttag gtggagaaat gccaactatg gctatcaact tctctcatag
     3001 tgcagcacaa ctaattgggc aatactacaa ctttgtacgc tatggctttg atggttatcg
     3061 tgatattcac caaagaactc atgatgttgc tgtttattta gccaaagaga tcgaaaaaac
     3121 tggtattttt gaaatcatta atgatggatc agaattgcca gttgtgtgct ataagctgaa
     3181 agaagatccc aatcgcgaat ggacactata tgatttatct gatcgtctgt taatgaaggg
     3241 atggcaagtc ccagcctacc cattacctaa agacttggat caattaatta ttcaacgctt
     3301 agttgttcga gcagactttg gaatgaacat ggctggtgat tatgtacaag atatgaacca
     3361 agcaattgaa gagttgaata aagctcatat tgtttatcat aaaaaacagg atgtgaaaac
     3421 gtacggcttt actcactaaa attcttcaga gaaatataaa agaagttaat taactaataa
     3481 aaggagttgt gattaccaca cagctcctta aatctatttg aataaatata tgatattcat
     3541 ttgttacact ctcaa
//
LOCUS       DAZUKA010000204         3507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000204 DAZUKA010000000
VERSION     DAZUKA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.209"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1006
                     /gene="galE"
                     /locus_tag="ACG8PN_002768"
     CDS             17..1006
                     /gene="galE"
                     /locus_tag="ACG8PN_002768"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4862875.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1018..2502
                     /gene="galT"
                     /locus_tag="ACG8PN_002769"
     CDS             1018..2502
                     /gene="galT"
                     /locus_tag="ACG8PN_002769"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4862876.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2526..3506
                     /locus_tag="ACG8PN_002770"
     CDS             2526..3506
                     /locus_tag="ACG8PN_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4862877.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1186 a          641 c          775 g          905 t
ORIGIN      
        1 caaaggagtg ttttttatga caattttagt actaggcgga gccggttata tcggttcgca
       61 cgctgtagac cagctagttc aaaaggggta tcaagtagct gtggtagata atttgctcac
      121 aggtcacaaa caagcagtac atccagatgc tcatttttat gaaggagaca tccgggataa
      181 agaatttcta cgttccgtat ttgaaaagga accgattgaa ggagtaatcc attttgctgc
      241 aagctcttta gtaggagaat ctgtcgaaaa accattgatg tatttcaaca acaacgtata
      301 cggtatgcaa atcttacttg aagtcatgca cgagttcaat gtgaataaaa tcgtgttctc
      361 ttctactgca gcaacttacg gagaaccaaa agagtctcca atcacagaag atacaccagc
      421 taatcctaaa aatccgtatg gtgaaagtaa attgatgatg gaaaaaatga tgaagtggtg
      481 tgaccaagca tatggcatgc gctatgtcgc tttgcgctac ttcaatgtag ccggagcaaa
      541 agcagatgca tctatcggag aagatcatac accagaaacg catcttgtcc caatcatttt
      601 acaagtagct cttggtcaaa gaaaagcatt ggctgtttat ggagacgatt atgatacacc
      661 agatggaaca tgtatcagag attatgttca agtagaagat ttgattgctg cacatatttt
      721 agctttggaa tacttgaaag aaggaaatga aagcaacttc ttcaatttag gcagcagcaa
      781 aggatactca gtcaaggaaa tgctagaagc agctagagaa gtgacaggaa aagaaattcc
      841 agcagaaata gcaccaagaa gagctggaga tcctagccga ttagtcgctt caagtgaaaa
      901 agcgagagag gttctgggtt ggaaacctga atacacagat atcaaagcaa ttatcaaaac
      961 agcatgggat tggcatgtca gccatccaaa tggttatgaa gaatagggga atgagatatg
     1021 tcaatcagtc aaacgatttg tgattttgcc acacttgcga tcgaagctgg cggatggatg
     1081 gaaatggacc gcttatattt acagaatcgt cttttaggga tggtaggaga agatgctcta
     1141 gaaagcattg aagtaagacc agttaaggaa gaatctgtag acttattgga tcagcttgtc
     1201 gctaaggcaa gagaaaatgg tgttctttca gaattgccag cagaacaaga gatttttgaa
     1261 gcgcaattaa tggattttct cacaccgcca ccttcagtcg tcaatgcatt ctttgcacag
     1321 cactattcga aaagcccaga ggaagcaact gagtatttct ttaatttaag taagaaaaat
     1381 gactacatca aaacaagagc catcgcaaaa aacatcaaat ttccggcaga gtcggagtat
     1441 ggtgaactgg aaatcacaat caatttatct aagccagaaa aagatccaaa acaaatcgca
     1501 gcagaacgcg aggcggtaaa agtagactat cctacctgca tgctttgcat ggaaaacgaa
     1561 ggatatagag gtcgcttgaa ctatccagcc cgcaccaatc accggattat tcgaatgaat
     1621 ctagacggtg aaagctgggg attccaatat tctccttatg cttattacaa tgaacattcg
     1681 atcattttat cagaacaaca ccgcccgatg aaaatcacaa aggaaacatt tgctagactg
     1741 cttcggatta ctgaagtctt gcctcattac tttgttggtt cgaatgctga cttgccgatt
     1801 gttggaggat cgattctttc tcatgatcat tatcaggcag gacgtcatga atttcctatg
     1861 gcaaaagcac cgatagaaaa acttatcaaa ctagcagatt atccgcaagt catcgcagga
     1921 gtcgtgaaat ggccgatgtc tgtgatccgt ttgcaatcaa cagagaaaaa attgttgatt
     1981 gaagcagcta actcagtatt ggaaaagtgg aaagcatatt cggatgaaac agtatctatc
     2041 agagcttttt cagcagatgg tacaccacac catacgatta cgccaattgc acgaagaagg
     2101 gacaattact ttgagttgga ccttgtcctt cgagacaaca atgtttcaga agaacatcct
     2161 gatgggattt ttcatccaca taaagatgtc caacatatca aaaaggagaa cattgggttg
     2221 atcgaagtaa tggggcttgc tgttttacca cctcgtttga aaccagaact agtagaagta
     2281 gagcatttct tattaggaga agaaaaccag atggctgaat atcatcgtcc gtgggcagaa
     2341 caattaaaaa aagaacatcc tgatttgacg aaagatacgg tcaccgatgt cgtacaaaaa
     2401 tctgtaggtc aggtttttgc acgtgtactg gaagacgctg gagtattcaa gcgagatgaa
     2461 aaaggacagg cagcgtttga acgatttact gcaattttat aaacgtaaaa aaggaggaga
     2521 gacaaatggc aacaattaaa gacattgcgc agttggcagg agtttctcct gctactgttt
     2581 caagagttct caactatgat cctgatcttt ccgttggctt agccacaaag caaaaaattt
     2641 ttgaagcagc agaacgctta aattatacga aacatcgtaa aaatgcagca aatactaagc
     2701 aaacgcttcg cctgatccag tggtacgacg atacggaaga gcttgaagat ctttattatt
     2761 tatctattcg tctaggaatc gaaaaaaagg cagaagaagc aaacgttact ttattgaaag
     2821 aaacgtggga ttccattagt gatcttccga cagatggaac gatcgcacta ggaaaattcg
     2881 acgaagctca gttattagaa atgaaaggat cccaaaatgc cttgcttttg gtagattctg
     2941 acggcacttc tcaaggaatc gactctctag ttgtcgactt taagtcaagt gttcaaaaag
     3001 tcattgaaca ttttgtctcc cataaagcgc aacatatcgc tatgcttgct ggaacagaat
     3061 atacgaagaa aaaccatcag cggctaaaag atcccagaat tatggcattt caacaagttt
     3121 tggcagaaaa aggctacgac gaaggtccaa ttatcgaagc agatttttct gtcgagtcag
     3181 gatatcaagc agtgaagcag tatttaaaaa cgaatccgca tgttcctgat gcgctttttg
     3241 cagctaatga tgcattagcg attggcgcac tgaaagcgat tcaagaaaaa ggtttagtgg
     3301 taccagaaga tatttctgtg atgggtttca atgatgtcag tgtagcgaaa tatgtaacgc
     3361 cctctctttc tactgtaaaa gtttataccg aatggatggg agagctagca gtggagacga
     3421 ttcttcaatt aagtaattcc aaagcaccgg tttcaagaaa gattattgtg gaaacagagt
     3481 tgattattcg agaatcaacg aaataaa
//
LOCUS       DAZUKA010000205         3480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000205 DAZUKA010000000
VERSION     DAZUKA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.210"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8PN_002771"
     CDS             complement(<1..182)
                     /locus_tag="ACG8PN_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4862878.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8PN_002772"
     CDS             517..951
                     /locus_tag="ACG8PN_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4862879.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8PN_002773"
     CDS             974..1294
                     /locus_tag="ACG8PN_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862880.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8PN_002774"
     CDS             1435..1626
                     /locus_tag="ACG8PN_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862881.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8PN_002775"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8PN_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4862882.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8PN_002776"
     CDS             2112..2375
                     /locus_tag="ACG8PN_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862883.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8PN_002777"
     CDS             2399..2587
                     /locus_tag="ACG8PN_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862884.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8PN_002778"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8PN_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862885.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8PN_002779"
     CDS             3070..3441
                     /locus_tag="ACG8PN_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4862886.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1298 a          497 c          699 g          986 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
//
LOCUS       DAZUKA010000206         3476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000206 DAZUKA010000000
VERSION     DAZUKA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.211"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..221)
                     /locus_tag="ACG8PN_002780"
     CDS             complement(<1..221)
                     /locus_tag="ACG8PN_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862887.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFP"
     gene            complement(377..622)
                     /locus_tag="ACG8PN_002781"
     CDS             complement(377..622)
                     /locus_tag="ACG8PN_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4862888.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            727..1500
                     /locus_tag="ACG8PN_002782"
     CDS             727..1500
                     /locus_tag="ACG8PN_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862889.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(1553..1867)
                     /locus_tag="ACG8PN_002783"
     CDS             complement(1553..1867)
                     /locus_tag="ACG8PN_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862890.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         1092 a          584 c          456 g         1344 t
ORIGIN      
        1 ggaaaccaat ctttaataat ttcgtctgca tctagaattt tgctgtcctt agaataataa
       61 tcaactaaat cattttcact ttcaaataga ttttctattc tcttgttaat agatttttgg
      121 ttgtgatcta tttgatttac tacatgtgta tatgattgcg agcttgagga gaatgcattt
      181 aagtcagcat ttgtaaaatc caattcatat gccttataca aggatatccc tccattcttg
      241 tattttgatc cacaatttat taggcaacat aacagtatac cctatcgtta catatcccat
      301 tcattattat accagtattt cttcgtgaca cataagggtg taacataata atttcaaacg
      361 aaatgactac aagtagtcat tgaagcaaat cttcataaga cacctgtaga atatctttga
      421 tggctttcaa ttgatccaac ttaatgtgtt ggcggccacc ttcaatctta accaacgttt
      481 ccctagttat agcaatttta cgaagctgta ggtctctaat cagctcggtt tgaccaattc
      541 ctttttctaa tcggactcgt ctaatattgc taccgattac attttcttta tcaataattt
      601 tttcctcttt caagaatagc acaccttttg gactaaaatt agtccttttt cttattaatc
      661 ttaaagattc ggtatgctac aatgggacta gtattagtcc atattcaatt ttaaggagga
      721 atttttttga agaaatttgc aatcattcta acttgcgctt tactcatcgg aactataagc
      781 gggtgtacca cttctgacac aagttctaca agtacggaaa ctagtacaac aacctcagaa
      841 aagaattacc caatacattt ttttccggtt ccagatggat ttgaagctta ttatcatgat
      901 aaaaaactag atgttccttt atcagaaact gaatctactg aaaagatttt gccaattgaa
      961 attgaaagaa atcaagacga tataaaacta aaagctgatc ttcccttact gaacagcaca
     1021 caaattgata gacatgaaaa tattaaagtt aatattgaaa aaggagcgtt agcacaactt
     1081 tatactgaaa aagttgaaca attttttgag gctggtgcca ataaagactt ttcaaaaata
     1141 agcgattatt cggataactt ttttaatgag acccaagatg aaatcacgaa ctcaactttt
     1201 atggctgaag atatgtacaa tatgaagttg aaaaaaataa agtcgtatga ttatccatta
     1261 aatctaacat taaataaagg gatactctct tttgtattag caggttacgt tgactatgaa
     1321 tatacagcag attatttgga gacgcccctt caacacgata ataacgatat taattttaga
     1381 tttatctaca attcaaaaag taaaacatgg ttagttgatc aattgagcac tataaatacg
     1441 ggatttcatg aattacctaa gcctgacaat gaaactgctt tggtcattaa aacattctaa
     1501 aaaagaaacg ttgaaggaat aattattctt tcaacgtttc ttttattgtt tttcagttta
     1561 cggtcttacc tgagcgaata atctgatttc taaatgattg gaaaacttct tgtggttgat
     1621 aataatctgt atctatttca aatgtttctg agaataattc attgaatttt tctactgaaa
     1681 tgatgatttt accattctcc tctcgactgt tttctattat ttctgcctct aattcaaata
     1741 aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa tcaagaaaaa
     1801 gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact ttattttcat
     1861 cagccattct tccaatttcc tcactttcat tttcaattgt ccattgttgc ggacgtatca
     1921 gattttctct atcatttttc agtgtataga tcacttgtaa tacacaacaa tgctttgtaa
     1981 acttattttt ttagttagtc gaatcttttt aaatgtgcgt ttcataccat gcgcttgata
     2041 tattttctcc tattttttaa atcgtttatc acaactttta tcgttttttc ttctgaaaaa
     2101 aatcttgttt acatcgttac atagtttcca aaaagataca ttactagtag aaacaacaag
     2161 attaatttta cttcccactg aaatgggaag tatattttaa atattttttc tccttcaact
     2221 tacactataa agtatcttaa tcacacgata tgctagccac taaattcatc atatttgatt
     2281 ccttttttaa gataatgtag catcttttta tccaataacg caattgtttt taaggataaa
     2341 tttcttaaga tggtacattt caaaatggca tttgacatat gaaacaaaaa agaaagaaaa
     2401 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtt ggctgtgcca
     2461 actggttttc tttcaaaatc atttcataat ttttgatatc ataaaaaaat ccattttgga
     2521 cctattttcg gtcataattt agtacctact tttggtcata gtttcgtccg tagttttggt
     2581 catagtgact aaggcttttc tgttcacaaa attggtttaa atttgctttc tttaacactt
     2641 catttatatc atagcaatta taatgtttac acaacatcat tcctaatttt aacggttgtt
     2701 cctctttata ttttttaagt tctacatttt tttgcgttta attacttttc tcttttcttt
     2761 atattccgct ccaactttct ctttaatagt gataatagaa ttacatattt ttgttaaact
     2821 tccttaaaaa aattggatct cgattactca aatttgcttg caggcttttc taattgcttt
     2881 gttttttctt cctaattatt tgattttatt tccattttct tctagtgaaa atttattatt
     2941 tttaataatc gtacgcgtag aactgatttc attttttgta tctgtttgat tatctaacgt
     3001 ttgaaattca aagttttgat tggctttatc aatatctttt tatactactt tatttgcaag
     3061 atatattttc ttgcttaatt tggtcatagt ttcattcgtc acttcggtga tattttttta
     3121 atttgcaaat aaaaagagtt aaaccgatat acattaggtt caactctttt gcaggtttca
     3181 tgggtgtact atcactttta atctgataga tgtaaacctc cctttatctt tttgttactt
     3241 caattttcaa caatagctcc tttataggtt gtcaatattg ctctacgtat tctggctttt
     3301 taacccatta tttctctaaa gtggaagaga ataatcgctt aatccccact aattccttgt
     3361 cgtaaattca tgtcaaatac aatttttaaa agccatttaa ttcatggatt atctcttcac
     3421 ttttttctag ataacaatat ttcaagttgt tttttatctc atgtttcatc tttaat
//
LOCUS       DAZUKA010000207         3473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000207 DAZUKA010000000
VERSION     DAZUKA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.212"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8PN_002784"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8PN_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4862891.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8PN_002785"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8PN_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          974 a          769 c          549 g         1181 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt cta
//
LOCUS       DAZUKA010000208         3469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000208 DAZUKA010000000
VERSION     DAZUKA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.213"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..2711
                     /locus_tag="ACG8PN_002786"
     CDS             42..2711
                     /locus_tag="ACG8PN_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4862892.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2802..>3469)
                     /locus_tag="ACG8PN_002787"
     CDS             complement(2802..>3469)
                     /locus_tag="ACG8PN_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4862893.1"
                     /translation="LKMYTVIFFEYSDFFLFLINATDEILSVNKVFYTVPAEKMSRVS
                     SDINTITSRIGLTKAMHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDI
                     YDRNDLAMVLIGMPGIEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGID
                     IKLEDFSDYEAITTIIKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLV
                     IGI"
BASE COUNT         1024 a          647 c          651 g         1147 t
ORIGIN      
        1 aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa aatttaaagt
       61 cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg tttttgattt
      121 tagcacttgt ttatctaagt atcgggatct atcctggaag cagcagaagt attttggcaa
      181 gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt cttcttggaa
      241 agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta tctttaattt
      301 cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac cagatgatgc
      361 cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg gctttttggt
      421 ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc gtcagttacg
      481 cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat gcatttgttt
      541 atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg ccgaaacttt
      601 tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt atgattggcg
      661 tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa tataaaaaaa
      721 cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg atcattgtat
      781 tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc actacgttga
      841 aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt gtttatgaca
      901 cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta gtcctctgtc
      961 tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac ggcagtcttt
     1021 tcgccatact tatcgcaagt ttctatcttg ttccattgaa tttgttctgg catggtatgc
     1081 atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta gtgctcctgc
     1141 ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg ttaggaatca
     1201 ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa ggaagctaca
     1261 cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt tatgcttttg
     1321 ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata ttgttattat
     1381 tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc attcttgatg
     1441 attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg aaagctcttg
     1501 taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg gatccagtat
     1561 ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct tctataagaa
     1621 atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg acaaatttga
     1681 atatccgtta taacaataat actttgctaa tggatgggtt tacaggaatc aaatataaca
     1741 tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt caaagtggcg
     1801 actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca ccgttgtccg
     1861 ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac ttgatcaata
     1921 cattgtctgg tttaaatgaa cagtatttta cattctatac acctactttg aagaatcagt
     1981 tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca gcagataacc
     2041 agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct tacttaagtt
     2101 tgttcccaac caattttgca caattagaaa gttctagtgc gacgatcact gtcaatggta
     2161 catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc tattatcctc
     2221 aagagacaac agtgacattt actgcaagtt tttatggaac gaaagaagta agtttcatgg
     2281 agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat cagatcaaag
     2341 aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta aataccgctg
     2401 aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct aaaattgatg
     2461 ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa gttccagcag
     2521 gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt gcaatccttt
     2581 ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc aatcgcccct
     2641 tttatggcac gattgatcca gcaatagaag aaaaaccgcg aaatacacac agaagaagac
     2701 gaagaaaata aagaaacgat ggaatctgtg tcactcttct atttaaatgg caaaaatttc
     2761 aatctttaaa tgacaaaaac cacctgattt ttggtggttt tttatatacc tatgactaaa
     2821 ctatctcttg ctgcttcgac aacttctaca gtgatcttat ccatttgatt gattcttaaa
     2881 atccgatcaa tctgcgcaaa taaacgatga ataagcctaa aatttccttt tgtgatctta
     2941 atgatagttg tgatagcctc ataatcagaa aaatcttcta gcttgatatc aattcctaaa
     3001 tctgcccatt tgtattccaa aatatgatgt gtttcgtcct tactaaggtt atcaaattca
     3061 tgtgcaaatc ctatgcgaga gtaaagctgc ggataacgag acaatctttt ttcaatacca
     3121 ggcataccga taagaaccat tgccagatca tttctatcgt aaatatctct tagctgttct
     3181 aaatgctgaa gctttaaacg atcaatttca tcgacaataa ttaagtcaat tgcttcatag
     3241 gttttaggca caggaggatc ttcttgattg tattttagta ccatatgcat tgctttagtc
     3301 agacctattc tactagttat tgtgttaata tccgaactga cacgagacat cttttcagct
     3361 ggtacagtat aaaaaacttt atttacactt aatatttcat ctgttgcatt tatcaagaac
     3421 aagaaaaagt ctgaatattc aaaaaaaata acggtgtaca tttttaaaa
//
LOCUS       DAZUKA010000209         3436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000209 DAZUKA010000000
VERSION     DAZUKA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.214"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..734)
                     /gene="nth"
                     /locus_tag="ACG8PN_002788"
                     /pseudo
     CDS             complement(57..734)
                     /gene="nth"
                     /locus_tag="ACG8PN_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(741..1439)
                     /locus_tag="ACG8PN_002789"
     CDS             complement(741..1439)
                     /locus_tag="ACG8PN_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4862894.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1707..2891
                     /locus_tag="ACG8PN_002790"
     CDS             1707..2891
                     /locus_tag="ACG8PN_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4862895.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2897..3340)
                     /locus_tag="ACG8PN_002791"
     CDS             complement(2897..3340)
                     /locus_tag="ACG8PN_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4862896.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          870 a          696 c          584 g         1286 t
ORIGIN      
        1 tttctccata ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac
       61 aggcgtggtt tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga
      121 catattgata gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcaa
      181 ccaaaaaaaa tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc
      241 agtgtttctt ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag
      301 acacgttcca cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg
      361 acatttgctg tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc
      421 acttgcccat cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta
      481 tttcgataca aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct
      541 aacgcatcgg gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca
      601 tctgtcgcct gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg
      661 agttctccat gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt
      721 tgtttgctta acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat
      781 tcttcttgtt gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt
      841 tggtcttctt ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg
      901 cggtcaacgt aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc
      961 aaaatttccg gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc
     1021 gaagaaagcg ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt
     1081 tcttgtgttt tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca
     1141 tagatggggt aaagattata tcgatctgct tttttaccgt tgacaatcgt cgtttctatc
     1201 ttgataaatc catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt
     1261 cccatatcca tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata
     1321 tgcaactgta gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta
     1381 agtaaaagat tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg
     1441 atttctcctc cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt
     1501 cttttctatt tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt
     1561 gttcatgttt ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct
     1621 gttccttaga atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta
     1681 acattattgc ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt
     1741 ttggatacgt ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc
     1801 cgtttttaac gctgccttat tctcactcca tacatcagca agctttctat atcactttgc
     1861 tcatgtctgt ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg
     1921 atcattttgg gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt
     1981 tgctttttgg tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat
     2041 tgacggcagg agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg
     2101 aaagaaccaa agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc
     2161 ctattatagg gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac
     2221 tctttacttt cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa
     2281 aaaaacgttc agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat
     2341 tattcaagat ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag
     2401 ctggctctct tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg
     2461 ggttggttgg tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca
     2521 ttatgcctcg attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca
     2581 gtgaaatcct cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt
     2641 ttttatttgg gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg
     2701 gccaactatc caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa
     2761 gtatacaatc tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag
     2821 caccatttct tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca
     2881 ataaaaaata gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac
     2941 gaaatactgt gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta
     3001 cccatttttg atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt
     3061 gaaaatagat ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta
     3121 tcagtttttt tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt
     3181 ttgtcacgtt aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag
     3241 taaatcctgc aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta
     3301 aatccagata ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt
     3361 tcattatatt gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc
     3421 caatctgaaa ccaaga
//
LOCUS       DAZUKA010000210         3414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000210 DAZUKA010000000
VERSION     DAZUKA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.215"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1124
                     /gene="pplA"
                     /locus_tag="ACG8PN_002792"
     CDS             189..1124
                     /gene="pplA"
                     /locus_tag="ACG8PN_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS4862897.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1214..2317
                     /locus_tag="ACG8PN_002793"
     CDS             1214..2317
                     /locus_tag="ACG8PN_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4862898.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2330..3283
                     /gene="menA"
                     /locus_tag="ACG8PN_002794"
     CDS             2330..3283
                     /gene="menA"
                     /locus_tag="ACG8PN_002794"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4862899.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         1150 a          608 c          704 g          952 t
ORIGIN      
        1 actccttttg gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa
       61 agaaaacgtt tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa
      121 aagattagaa tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg
      181 gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc
      241 tagtgttagg cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat
      301 ctacagcaca atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg
      361 gcggagattt gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc
      421 gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg
      481 tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa
      541 aatctggcac aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac
      601 aaagcgcaag cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa
      661 actacgcaca acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg
      721 acaatggtgc tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg
      781 gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg
      841 attatatcga caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga
      901 aagcaaaaag cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca
      961 aagcaatggg cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac
     1021 acagctcaca ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg
     1081 acactcaaac aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta
     1141 ataaaagttg gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga
     1201 agagaggaaa aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt
     1261 tatggtgcta actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc
     1321 taccaagata ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg
     1381 tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa
     1441 agaacttggt gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga
     1501 gcaagctggg atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc
     1561 ctacgaatat agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca
     1621 attatggcga atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc
     1681 attgaaatta gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct
     1741 aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga
     1801 agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa
     1861 tgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca
     1921 agatccgaac atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt
     1981 agttacctct ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt
     2041 gtttgatcca aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac
     2101 agataaatca attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa
     2161 aggactggaa tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa
     2221 agacgataaa gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc
     2281 ccattacacg atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa
     2341 aatcttttta gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt
     2401 aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt
     2461 ttttattgga atgctagttt ttgatttagc gactaccgct atcaataatt acatggatta
     2521 tgaaaaggct cattctgaag tatataagta tgaagaaaat gtcattggcc gcagaaatct
     2581 atctcctgta ttgattcgaa acatgatttt agctatgatt gcatttgttt ttgttatcgg
     2641 cgttattcta acgatcaata cgggttggct tttacttttg atgggattta tctgttgttt
     2701 tattggagta ttctatacat tcggaccgat tcctttatct cgaatgcctt taggggaaat
     2761 tttcagtggg gtgactatgg gccttggaat ttttgccatg gtcgtttatg tcaatacatt
     2821 cggttcagat gtctatgatc tgacagtcaa cttttcatct ggacaatttg ctttggtagg
     2881 aaatctttgg gctatagcag caatcgtatt agcttcctta cccttggtgt ttacgattgc
     2941 caatatcatg ttggctaata atttgcggga tcttgaccgt gatattgaaa atcatcgcta
     3001 tacactagtt ttttatatcg gacgtcctgc gggaacagtc ttattccagc tattgatgta
     3061 tgcttgctat ttcgtgattt taatggggtt gttgagcggt atctatgaat ggccgatctt
     3121 ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg gatcaattca agcaaacgtt
     3181 accccagcct atcagtttca gctattccat caaaaatatg gtcttattca atgccagtta
     3241 tgtattgggg ctattactaa caatcattat gcaagccatt taacagcatg aagaggttgt
     3301 gaaaaaactg ccctgcatca agtaataaga acagccaaat aaaaatagct ccttatattt
     3361 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt atca
//
LOCUS       DAZUKA010000211         3409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000211 DAZUKA010000000
VERSION     DAZUKA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.216"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..1000
                     /gene="rpsB"
                     /locus_tag="ACG8PN_002795"
     CDS             218..1000
                     /gene="rpsB"
                     /locus_tag="ACG8PN_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4862900.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1099..1980
                     /gene="tsf"
                     /locus_tag="ACG8PN_002796"
     CDS             1099..1980
                     /gene="tsf"
                     /locus_tag="ACG8PN_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4862901.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2116..2838
                     /gene="pyrH"
                     /locus_tag="ACG8PN_002797"
     CDS             2116..2838
                     /gene="pyrH"
                     /locus_tag="ACG8PN_002797"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4862902.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2841..3398
                     /gene="frr"
                     /locus_tag="ACG8PN_002798"
     CDS             2841..3398
                     /gene="frr"
                     /locus_tag="ACG8PN_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4862903.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1252 a          588 c          761 g          808 t
ORIGIN      
        1 tgaattcttt ttatctttca ggtaaactat tgaatgttga gcagaaaagt atatttgctc
       61 ttgcgatacg gatcttttgt ctgtataata gctgagggtg ctttatgcat cagcaaatcc
      121 acattcttat tggattgtcg gtaggtgctt gaaaaagttt gccggcaagg tagaagataa
      181 gaagaggaaa aataaaacca aatcggagga aacaaacatg gcagtaattt ctatgaaaca
      241 attactagaa gccggcgtac actttggtca ccaaactcgt cgctggaatc caaaaatgaa
      301 gaaatatatc ttcacagaaa gaaacggaat ctacatcatt gacttgcaaa aaacagttaa
      361 attagtagat gcagcttatg attacatgaa gagcgttgct gaagaaggcg gcgtagcatt
      421 attcgtaggt acaaaaaaac aagcacaaga agcaatcaaa gaagaagcaa ctcgtgcagg
      481 acaatacttc gttaaccatc gttggttagg cggaacattg acaaactggg atactatcca
      541 aaaacgtatc agccgtttga aacaaatcaa tgcaatggaa gaagatggaa cttttgaagt
      601 tctacctaaa aaagaagttg ctggtttgaa caaagaacgt gaacgtcttg aaaaattctt
      661 aggcggtatc gctgatatgc caagaatccc agatgtaatg tacatcgttg acccacgtaa
      721 agaacgtatt gctgttcaag aagcacacaa attaaatatc ccaatcgtag ctatggttga
      781 tacaaactgc gatccagatg aaatcgacgt agtaatccct tcaaacgacg atgcaattcg
      841 tgccgttaaa ttgattactt caaaaatggc tgatgctttc atcgaaggaa accaaggtga
      901 agatcaagta gttgaagaag atttcacaga agaaaacaca gcaacatcaa tcgaagaaat
      961 cgtagatgtt gttgaaggcg acaattcttc agcagaataa ttttgaatga tggagctgtt
     1021 tcaaaggctg ggcgacaagc ctcactctcc tttgaagcag ctttttttaa aaagaaaaac
     1081 acacaggagg agttaaacat ggcagacgtt acagctaaaa tggtaaaaga actacgcgaa
     1141 atgactggcg taggaatgat ggatgcgaaa aaagcattag ttgaagtaga aggcgacatg
     1201 gaaaaagcag ttgatctttt acgtgaaaaa ggaatggcaa aggctgcgaa gaaaaatgac
     1261 cgcattgcag ctgaaggatt ggcttcagtt gctgtgaaag gtaacactgc agcaatcgtt
     1321 gaagtaaact cagaaacaga ctttgtttct aaaaacgaaa tgttccaaga tttagtaaaa
     1381 gaaattgcag aacttgttgc tgaaaacaaa ccagcagaca tggaagctgc aatgaaaatc
     1441 aaaacagata aaggtacaat tgaatatgac ttgatcgaag caactcaagt tatcggagaa
     1501 aaaatcagct tccgtcgctt tgaagttgta gaaaaagacg acaatgctgc atttggtgga
     1561 tacttgcata tgggcggacg tattgccgta ttgactgttc tagatggaac aactgacgaa
     1621 acagttgcaa gagacgtagc tatgcacgta gcagctatca atcctcgtta tgtaaacgaa
     1681 tcacaaattc cagaagctga attagaacat gaaaaaactg ttcttacaga acaagcattg
     1741 aacgaaggca aaccagcgaa tatcgttgaa aaaatggtag aaggacgctt gaagaaattc
     1801 aaagcagaaa tcgctttggt ggaccaacca ttcgttaaag atcctgatat gactgttgaa
     1861 aaatacgttg catcaaaagg cgcaactgtt aaaacattcg tacgtttcga agttggcgaa
     1921 ggtatcgaaa aacgcgaaga taactttgta gaagaagtta tgagccaagt gaaaaaataa
     1981 ttctgttgaa taacagacgg gaacgcacgg tttgtgcgct cccttttttt aagcaaaaca
     2041 agaaaaacca gtcttagagt gtcgaaatca tctaagtatg gtaaaatggc aatagaaaat
     2101 attggaggaa tgaacatggt cacaccgaaa tatcagcgtg tagtcttaaa attgagtgga
     2161 gaagctttag ctggggatgc aggatttgga atcaaaccac cagtaatcaa agaaatcatc
     2221 caagaaatca aagaagttca cgagctgggc gttgaaatgg caatcgttgt gggtggcgga
     2281 aatatctggc gcggacaaat cggagcacaa atgggcatgg aacgtgctca agctgattac
     2341 atgggtatgt tagctacagt tatgaatgcc ttagctttac aggatacgtt ggaaaacgtg
     2401 ggtgtaccga cacgcgtgca aacttcgatt gaaatgcgtc aaatcgcaga gccatatatt
     2461 cgccgtaaag cagaacgtca tttagaaaaa ggacggatcg ttatctttgc aggcggtacc
     2521 ggcaacccgt acttctcgac agacacaaca gcagcattac gagcggcaga aatcggcgca
     2581 gatgttatcc tgatggctaa aaacaatgta gacggtgttt attctgctga tccgaaagta
     2641 gatgcaaatg cagtaaaatt tgaagaactg actcatctgg aagttattgc caaaggatta
     2701 caagtcatgg attcaactgc aagttcacta agcatggata atgatattcc actgcttgtc
     2761 ttcaatttaa atgaacacgg aaatattcgc cgtgcaatct taggcgaaaa tatcggaaca
     2821 actgtaaggg ggaaataaaa atggcagatg caattatgac agaagcaaaa gaaaaaatga
     2881 aaaaagcagc acaaaatctt caacgtgaac ttgggcaaat ccgcgcaggc cgtgcgaacg
     2941 ctagcctttt ggaccgtatt actgtgaact actacggtgc gccaacacca ttgaaccaaa
     3001 tggcatcaat ccaaattcca gaagcacgtg ttttaatgat cacacctttt gacaagagca
     3061 ttcttcaaga tgttgaaaaa gctattatgg caagtgatat cggaatcagt ccaaccaatg
     3121 atggaaatgt cattcgctta gttatcccgc aactgacaga agaacgcaga aaagaattag
     3181 ctaaagatgt gaaaaaagaa gcagaaaatg caaaaatcgc gattcgtaac atccgtcgtg
     3241 atgcgatcga tgaatataaa aaacaacaaa aaaatggtga tattaccgaa gatgatttgc
     3301 gcggattaga aaaagacgta caaacattga cagataacag cattaaagaa gtagataaga
     3361 tcgctgctga taaagaaaaa gaattattgg aagtttaata aaatcataa
//
LOCUS       DAZUKA010000212         3388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000212 DAZUKA010000000
VERSION     DAZUKA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.217"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..928)
                     /locus_tag="ACG8PN_002799"
     CDS             complement(65..928)
                     /locus_tag="ACG8PN_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4862904.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1124..3223)
                     /locus_tag="ACG8PN_002800"
     CDS             complement(1124..3223)
                     /locus_tag="ACG8PN_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4862905.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT          759 a          673 c          577 g         1379 t
ORIGIN      
        1 aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa catgcaaggg
       61 attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta atcaatcctt
      121 gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca aaagctttca
      181 tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca gaacgttcta
      241 aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt ggcttcaatg
      301 aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc tgctcgccta
      361 gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa tcttccgtta
      421 aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct ttgtttttca
      481 tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct aaactgcccc
      541 aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct tttttaggcc
      601 atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta tctggaagac
      661 cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca aaagcgtaat
      721 gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt tgattttggt
      781 aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg cttaagccaa
      841 aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata ataatacctg
      901 taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa aggttttctt
      961 aatacatttc tttgctatca taacataatg ttatctactt gaatagctga gccttggttt
     1021 ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt tcgaataaac
     1081 ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg ctcccatttc
     1141 ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa gcactttctc
     1201 caatgccttt catcaatgca gattttgttg aagtggcata tagattataa tagccttccc
     1261 cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat ccacgttgga
     1321 tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct ggattagatg
     1381 catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag tttgaagagg
     1441 aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg
     1501 cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct gcttttttcg
     1561 cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg agcatcttat
     1621 ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc actttttcta
     1681 ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact tttccgtctt
     1741 taaagactat agctgttttt tctttagctg tttgcaattc agcatattga tttttagctt
     1801 ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg attgcttgga
     1861 tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg atggcaggtg
     1921 ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata tcttggtaag
     1981 ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca ttttttagtt
     2041 ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca tctgtataaa
     2101 aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct tctgctgcta
     2161 cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca ccgagtagaa
     2221 gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc gctttatctg
     2281 gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc acgattgtta
     2341 ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct atttttgctt
     2401 caagatcagt accctctttg cttacagatt gattttcagc atcggattca ctatttattt
     2461 catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg
     2521 gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca gttttttctg
     2581 tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg ttttctctat
     2641 cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc tgtgcaattt
     2701 tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct tcacgatgtg
     2761 ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt tctttcagtt
     2821 cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt cctgattctt
     2881 ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta gttacttgat
     2941 cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc attacagatt
     3001 ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct gtatcaggaa
     3061 agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt tcttcaattg
     3121 attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga cagatagtat
     3181 tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag cgatcggctc
     3241 ctttactagg tttacgctac catttaatct atcatattct atacgtcacg ccaatagctt
     3301 atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa aaaaagcttg
     3361 agactgcgta gtctcaagct ttttttct
//
LOCUS       DAZUKA010000213         3345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000213 DAZUKA010000000
VERSION     DAZUKA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.218"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1665)
                     /locus_tag="ACG8PN_002801"
     CDS             complement(82..1665)
                     /locus_tag="ACG8PN_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862906.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1787..2371
                     /locus_tag="ACG8PN_002802"
     CDS             1787..2371
                     /locus_tag="ACG8PN_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862907.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2382..2627
                     /locus_tag="ACG8PN_002803"
     CDS             2382..2627
                     /locus_tag="ACG8PN_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862908.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2633..>3345
                     /locus_tag="ACG8PN_002804"
     CDS             2633..>3345
                     /locus_tag="ACG8PN_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862909.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQS"
BASE COUNT         1160 a          462 c          524 g         1199 t
ORIGIN      
        1 atgctattgt gagaatataa ctaattagtt taatttgaaa gtaaatatac gctataaaag
       61 tgcatcttgt gttataaaaa attattttat tttgtgaaca taatttaaca attctttctc
      121 ggaattgatt tcttttctta aatagtcttc agacttatta ataatattat ctattatgtc
      181 tagatacata tcacttttcg attcagctaa ttcatacagt ttttttaatt ctttatcctt
      241 taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac tataatgttt
      301 acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa taaaaggcgg
      361 aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta atttgttaaa
      421 ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct tatctgctaa
      481 cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca tctctttttc
      541 accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta gcgaagaaac
      601 tctcattttt tgctggagta taggatcaac tagactattt aagtgatttt gtaattcttc
      661 attgttgttt cctagaactg caagggaacg taaacggtag taatattcta tttctgttag
      721 tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa ttgtctcgtc
      781 tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa agaacccttt
      841 tgtatacgtg atctgttgat tttcaccaag tgatttatta atttcctctc ttgattcagc
      901 tgtgaaccta tactttgcaa tttctttttt tagaatattt aaatagaatt ctttattatc
      961 aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg cttcctcatt
     1021 tccataagat aattgaaaaa caaacttaaa ggctgtctta cataggtttt ctttggcttt
     1081 ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt caggtgtaac
     1141 agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt tttttcctaa
     1201 tattggattt tttaggaata taatttcata ggacttccgt ataacatcct gacaagtttc
     1261 ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag gatgagctga
     1321 ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat caattagctc
     1381 agttgaacta taagcttttt ctagtagttc catttcccat tttggagagt ttggatcact
     1441 ttttcttagt ctagctatat cttctaaaat tttcttagaa gtattatcct cgtaaatatt
     1501 tgctaaatca gataatttat caattaaatc taaaataagc gcactgtaat tgacagctat
     1561 tgcagaacga taatttccct gataaaaact acttctaact tcttctaata atggaataat
     1621 tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa tctccttttt
     1681 ttatttgtat gacaagttta ccataaaaaa aaattaaaaa gcgagacata tttatgttgt
     1741 tgtgttatca taataacata aatatgtaga gtgagatgat gaaaaaatgg atttgaaaca
     1801 atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg atatttttgt
     1861 agttagcatc ttattgtatt tattttatct aattttcttt cacaatttaa tcgggtacat
     1921 gattcttgat tggaaaggaa tgttcgatat agaaagaaaa ccatttgcta acaatcaaac
     1981 ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga tccatgaaat
     2041 tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaatga caacggatac
     2101 tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg atggtcaaga
     2161 aattatcaat ggtcgtgctg aaaaaacaat caattctaat actgaaattt ctttttgtat
     2221 gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa cagcctttgg
     2281 tgatattgaa acaagtatta aggaacttag ggaatatgtg cagaacccat ataattttca
     2341 atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa attataaatc
     2401 aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat tttactgtag
     2461 gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta tttagtcttt
     2521 atgcttggat tgtacaaatg gtttgggttt ccatattgat gctgttgttt tattggatag
     2581 tacgggttat tcttttattg ttaaacaaaa gaaaaaagga taggtgaata aaatgaaatc
     2641 aactaaattg aaggttgtat tgttatcgac agcagcatta ataaccctta cagcatgtgg
     2701 tacaactaat agtgctaata ataacaaaga cacaaagtct agtagtacga tggttactaa
     2761 aaacagcact acatcggcat caacaacaat gtcttcaatg actgaaaata atgctgaaaa
     2821 gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gatgaagttt ataacaactt
     2881 tattaataca gtaaaaaatg atgaaaatta tgggaatgat ccattgcaaa ataaaaatct
     2941 gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat caaaaatcat
     3001 gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata ttaccataaa
     3061 taaaccagct tacgagtatt acgcagttgc tgtagatatt actgatggtg tacctgagtg
     3121 ggtacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc caacagttgc
     3181 agtagtaaca ccaaagaaag ttaacggcac actatactgg gatgtaatgg gaatgacaac
     3241 aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt tgggagcata
     3301 taaattatat caacaatcag agcctgttat ggacgatcaa tccaa
//
LOCUS       DAZUKA010000214         3331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000214 DAZUKA010000000
VERSION     DAZUKA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.219"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..574
                     /locus_tag="ACG8PN_002805"
     CDS             110..574
                     /locus_tag="ACG8PN_002805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4862910.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            588..1337
                     /locus_tag="ACG8PN_002806"
     CDS             588..1337
                     /locus_tag="ACG8PN_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4862911.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1337..2167
                     /locus_tag="ACG8PN_002807"
     CDS             1337..2167
                     /locus_tag="ACG8PN_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4862912.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2167..2814
                     /locus_tag="ACG8PN_002808"
     CDS             2167..2814
                     /locus_tag="ACG8PN_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4862913.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(2912..>3331)
                     /locus_tag="ACG8PN_002809"
     CDS             complement(2912..>3331)
                     /locus_tag="ACG8PN_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFS4862914.1"
                     /translation="INVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYT
                     SLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFH
                     YSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1116 a          445 c          619 g         1151 t
ORIGIN      
        1 ctgaataact ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa
       61 ggtggcaatt tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt
      121 agtgcgtgta gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta
      181 tgatattaac catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat
      241 ggctttgaca attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt
      301 taaagggatt atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa
      361 ggaagctttt gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt
      421 gtctaagaaa agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt
      481 agaagccaca aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc
      541 ttcaagtatt gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag
      601 ccattttatt aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac
      661 aaaatattgg tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc
      721 atacagggat tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg
      781 gggcggctgg acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt
      841 taactaatag tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg
      901 aatttatgag tattatcatg cgcatgataa ttgctcaatt tggacatgct gcagataaag
      961 ccattgaaaa taataaattt agaaaagctc aaattattca tatttattgg tcatttattt
     1021 ttaatgcttt tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg
     1081 tggcagattt agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa
     1141 atatgctttc cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat
     1201 atccttactt tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg
     1261 tgacaatttt tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag
     1321 aagaggcgca tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga
     1381 tcttaataag atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca
     1441 aatgcatatg ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga
     1501 tgaagagaaa aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca
     1561 aatgacttca tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga
     1621 agaaggaaac tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt
     1681 tgctggaatt ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg
     1741 attgtctttt gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc
     1801 tataccttct attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa
     1861 ataccttact aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat
     1921 cattggtttg gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt
     1981 aaaattcact acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc
     2041 tggattatta ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag
     2101 tacaaatatg ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat
     2161 cttataatga ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt
     2221 atgtatcaaa agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat
     2281 tcttatcaaa aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat
     2341 gagggatgta acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag
     2401 attgattact tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag
     2461 aaaaaaataa aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt
     2521 accacttgta aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa
     2581 agtaaaccta atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa
     2641 gaatgtctgg tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg
     2701 aaggttcttg caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt
     2761 atttacgata ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt
     2821 tttttgatgt atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa
     2881 ttgattgctt tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat
     2941 ttttatgcac cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat
     3001 cacaacgaac tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct
     3061 tttctaaaat aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg
     3121 ttgcagataa accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt
     3181 ctagaagttt atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt
     3241 cttctgatgc gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt
     3301 taatattgta aactctatca gttacattga t
//
LOCUS       DAZUKA010000215         3306 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000215 DAZUKA010000000
VERSION     DAZUKA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.220"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..193
                     /locus_tag="ACG8PN_002810"
     CDS             <1..193
                     /locus_tag="ACG8PN_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862915.1"
                     /translation="NKNDKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIALVFLLIVI
                     IVSATFSIRFAKKIAEPKQ"
     gene            266..745
                     /locus_tag="ACG8PN_002811"
     CDS             266..745
                     /locus_tag="ACG8PN_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4862916.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            968..1111
                     /locus_tag="ACG8PN_002812"
     CDS             968..1111
                     /locus_tag="ACG8PN_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4862917.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1456..1584
                     /locus_tag="ACG8PN_002813"
     CDS             1456..1584
                     /locus_tag="ACG8PN_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862918.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            1689..3272
                     /gene="abc-f"
                     /locus_tag="ACG8PN_002814"
     CDS             1689..3272
                     /gene="abc-f"
                     /locus_tag="ACG8PN_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4862919.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
BASE COUNT         1074 a          666 c          705 g          861 t
ORIGIN      
        1 caacaaaaat gataaaaatc aaggattcct acaattttac ggagctgcat atgctgtttt
       61 aggcgtaatg ggtattttaa cagctttttt caatcaacgc tttattgcct tggtcttttt
      121 actgatcgta attattgttt ctgccacctt cagtatccga tttgcaaaaa aaattgctga
      181 accaaagcag taaataattc tctttttccc taaagtgttc tacaataaaa gagtacaata
      241 cgattgactt gaggagtgaa tgcttatgtt acaacaatac aaaaaaatca tggttgccgt
      301 agatggttct gatgaagctg aattagcttt taaaaaggcg gtcaatgtag ctatccgaaa
      361 taacggagaa cttctattag cacatgtcat tgacacacgt tctttccaaa ccgtttcatc
      421 ctttgatgga atgcttgcag aacaagctac tgaaatggca aagcaaacat tggcagatta
      481 tgaaagcaat gcgaagaaag ctggcttgaa taatgtaacc tctgtcgtag aatatggctc
      541 accaaaacaa attattgcca gagaaatccc agaagataat caagtagatt tgattatgtt
      601 aggagccaca ggattgaatg ctgtagaacg tttatttatt ggttctgttt ctgaatatgt
      661 tattagaaac gcagcttgcg atgtgcttgt tgtccgaact gatttagaaa accaattgcc
      721 agctaaagac cacaccaaag cttaacacgt agacgacctg ccaactatcg gcaggttttt
      781 ttgcacaaaa aaccgtgata aaaacaaaag acgcggcaag cactcatcgg tctttataat
      841 gtttaaaata aagggaaaac agtcaatgag ttcaagtaaa ataaaaaaaa gtgaacacca
      901 gggcgattag tcgccctggt gtggtttaaa acgacctaag catgccaaag cttattggtt
      961 cgtcaatgtg gaagtttccg atgaatctga tacgaaaaaa tatgaagtat cgatggcgga
     1021 taccgatttt atcgccaaac taaaatttta tttcgggttc gggtaccgcg agaaggcaag
     1081 tgctacattc ggtaaatcat tcaagaattg atgaaaggct accgcttgcc attgcaacca
     1141 caacgatact cctgaacgcg atgttggtga gatttccaaa gagaagagct gtttaatgca
     1201 acggtattaa gcaaatggca aaaggaagtc atgtagacaa agttttttat caaacaccat
     1261 atgatttcgc ctgaacgctg gtttagctca gaatacaatg atgtggtcca cgagacatta
     1321 ccctcagtca gataaaaaaa gctctctgac agaattacac atcgataaat attaagtaaa
     1381 tgtcagcgct agttgaactt tctgacactt tcaggtacac tggaaaagac aactacacga
     1441 aggagggaaa atactatgat gcgcatgact aaaataacaa gtaatgaaaa ttaccaacga
     1501 taccatatcg catttagcca tgcaattggt tttacagttg ctgttgacgg gacaagtttg
     1561 tccaaaacag ttgaattcac gtaaaatcaa tggccatctc aaggtatttt tctcatgata
     1621 atctgagttt aaataacaca ggccatggat aaccatggcc tttttgagtg gtctcaagga
     1681 gagagatcat gatcatacaa gcaatttcta ttaaaaaaac aatcggcggg aatccattat
     1741 ttgaggattt atcctgtcaa attgaagctg gtgaaaaaat tgtcttagtc ggtatcaacg
     1801 gaactggcaa atcaaccttt ttacaaatct tgaccggcag agaaggtgtc gacagcggtg
     1861 ttatcagtcg caaaaaaggc ttaaagatcg ggacggtcga acaagaactt acagtttccg
     1921 aagcgaccgt caaccactac ttgctccatt ctgccgctga gatacaggac ctcaaacagc
     1981 aaatgagtcg ctacgaagca ttgatgacag aaccagatac aaacttagag aagtgtctgg
     2041 cccgttacgg tgagctgcag catcgctttg aagaactagg cggttacgta ttagaagacc
     2101 ggattgcagc gatcttgcaa ggcttaggga ttccacaccg tcaggaagca cggttaacag
     2161 aacttagcgg aggcgaacga gttaaagtcg ctttagccaa aattttagca gccgatgccg
     2221 atttactttt actcgatgaa ccgaccaatc atttagatct taacagtatt cgctggttgg
     2281 aaaactattt acagaataca aaaaaatcgg ttctggtcgt ttctcatgat cggcaatttc
     2341 tcgatcaagt caccacaaaa acatgggaac tggaagaagg tgcattgatt gagtatcccg
     2401 gaaactacag ccgttttcga gtcttgaaag aagctcgctt ggctgagctg acaaaaaatt
     2461 acgaactgca gcaaaaagaa gtccagcggt tgaaagtcat gattcgtcgt tttcgtcagt
     2521 gggcacatga aggagacaat gagtccttct tcaaaaaagc caaagaactg gaacgccgcc
     2581 tggcgaaact gacactcgtc aaaccgccac ctcccccgaa aaatcggctg caaagcttgt
     2641 ctaatggtgg aaaatctggc aaagaagttt tcatcattca aaacttgcac cagcagtacg
     2701 ctgatcaagt actattcaaa gacagcagtt ttgcagtcta tcgtggagat catttagcca
     2761 tcatcggcga caatggtgca ggaaaatcaa ccttactcaa attattactc ggtgaggaaa
     2821 agccttacgg tggcaccatc aaattaggta gtagcttgca aatcggttat ttgccgcagc
     2881 agctgcagtt tgccgatccc gatagccgtt tgctggctta cgccatcaca ttgactggaa
     2941 acgaagaagt cgctcgacgg caattagcca aaagtggctt ttatcaaagc gatgtgggta
     3001 aacggatcaa agatttatcc ggtggtgaaa aaatccgttt ggcgttgctg aaactctttt
     3061 tagaaaaaat caacgtcctg attttggatg aaccaaccaa tcacctcgac agctatgctc
     3121 gagaagaaat tgaagaaatg ctgcaagact atcaaggaac gctcctagca gtcagccatg
     3181 accgctactt tttgcagcaa cacttccaag aagcactcgt tattgatcag gagcagatca
     3241 gccgccagcc gctcggccaa ctgattgact aggacgtttt ttcggctctt cgtcaaattg
     3301 tgttgg
//
LOCUS       DAZUKA010000216         3293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000216 DAZUKA010000000
VERSION     DAZUKA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.221"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..645)
                     /locus_tag="ACG8PN_002815"
     CDS             complement(148..645)
                     /locus_tag="ACG8PN_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4862920.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(775..1485)
                     /gene="budA"
                     /locus_tag="ACG8PN_002816"
     CDS             complement(775..1485)
                     /gene="budA"
                     /locus_tag="ACG8PN_002816"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4862921.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1498..3162)
                     /gene="alsS"
                     /locus_tag="ACG8PN_002817"
     CDS             complement(1498..3162)
                     /gene="alsS"
                     /locus_tag="ACG8PN_002817"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4862922.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          922 a          720 c          535 g         1116 t
ORIGIN      
        1 tgataataac attaaaatat gattatgaaa atcaataaca cttgtgattt tataaaaaaa
       61 taaaaaaacc gaaaaacaga aatgcccact gtctttccgg ctctttttat tcttgatttc
      121 tcttcttttt tgtttcaata gacgatttta gttattaata aatgaaaaag catttatatt
      181 aaaatttgct aataaaagat ctacatattt ttctagaaat gcttgatcga tttcatcata
      241 ccctttcgtt actccgcaat cgatatctaa aacaccgatt aaattacctt cttttaccat
      301 tggcactacg atttctgatt gtgcggctga atcgcaggaa atgtaatttg catgggtttt
      361 gacatcatca acgatcaatg tttcttgttt ttcagctgat tctccacaaa ctccttttcc
      421 catcttgata cgtgtgcaag aaactcgccc ttggaacggt cctaaaatca attcattacc
      481 atcgaataaa taatatcctg caaagacagt ctcaggcaaa gtctcattca acaatgcaga
      541 tgaatttgct aaatttgcta accagttatc ttctgcttct aataatcctt gttgctgtaa
      601 taataaaatt tcgtatgcat cttttttctg tttttctgtc cacatgataa aaaccgctcc
      661 tttttgatct atggagaaga ttatagctca gtttttttgt aaacgctagg ttttctcctc
      721 aaaaaaacga gatacgtcag gttaatcacc ttgaacgcat ctcgctgtct ttttctattc
      781 tgcttgttgg atttctgctg ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg
      841 atcatcaact ggaaaatgct gtacaaggga cgagatgcgg ctaatctcaa cttttccttc
      901 agccacttca aaatccatta catgtccgcc aaatgtatgt gtatcatcga taaaatgcag
      961 gtgaaatccc gctgcagcaa ctccttggaa taattcaggc gtaaagaacc cgactaatgt
     1021 tccttggaca ttgctttgtt ggaattctgg ttgagactct gaaatttttg ctagtcttct
     1081 gtaaggtttt tcttgcttcg gcatgatacg gatgtgcata tttttgaata atccagttat
     1141 cttcactgcc aaaaataggt tttccccggc ttctcttact aatatgtctt ttttcaattg
     1201 ttctccatct gcagtttttg agacagaaaa actagtatct ggcgtaaaat ctgtaattgc
     1261 tgcatagggg gtaagttcac ttccatctaa ttgggcaact gagccatctg atcttccttg
     1321 ataagctacg ccatctaaaa agatgacttc accatcaagc ccatgcaatg ttcccaatcc
     1381 cagagttcct ttttctaata tatgagcaat cgtttctgtt ccatccatca agccggccat
     1441 caaagctcct aatgtcccgt gctggtataa gattttttca gtcatgtcga cacttcctta
     1501 atagaattga tctggtaaga ttgtttctcc caatttctca ttatcacggt aatcaatcgg
     1561 aatatcaata attacgggtc cttctgtcgc aaatccttct tctaaagctt tttctaattc
     1621 ctctacactt gtagctcgga ttcctttagc gccaaaagct tctgcatatt tcacaaaatc
     1681 aactggacca aagtctacac ctgacgcccg ctgatatttc attttttctt ggaactctac
     1741 catattgtag tgtccatcat tccaaatcaa atgaacgata ttcaattttt tacgtaccgc
     1801 cgtttctaaa tcctgggcag agaataaaaa tcccccatcg ccggaaactg atacgatctg
     1861 tgtatttgga cgcaccaatg cagcagaaat cgcccatggc aatgcaacac caagcgtttg
     1921 cataccatta ctgaataaca aatgacgagg ttcataactt ctaaagtggc gtgccatcca
     1981 aatataatga ctacctacat ctacggtaac agtcatatca tctgttactt ttgactgaag
     2041 cgtgttaata acttctaaag gatgcaagat acctgcttct ccttttgatt gaacaatgtc
     2101 ccgctccgtt aattttgctt gaagagatgc taaatattct tttgcatctt ctgacacttg
     2161 ttgaggctct aatgaacctg tcaataaatc aagagtggct gagatatcac cgatcaattc
     2221 tctttcaggc tgcataaaag gatcgatttc agctggcgct tcatcgatca caatgatccg
     2281 cgcatctttt tccgcattcc agtttcttgc ttcatattcg attggatcat agccaattgc
     2341 aataactaga tcacttcgtt tcaaaagcat atctcctggt tggtttctaa ataaacccac
     2401 acgaccaaag aaatgcgctt ctaattcacg tgagatcact ccagctgctt ggaatgtctc
     2461 tacaacaggc aatgctgtct tttcaactaa ttgcctgatg gctaacgttt ctttttcact
     2521 tgatcctcgc atacctacta atagtaccgg tagttttgct tctctaatgc gttcagctag
     2581 gtaacggata tcatctgcag aagcagaacc taatttagga tcgctcaatg gcttgatgac
     2641 attaccttgg acaggtgcat ccactacatc ttgcgggata ctgataaagc tcgcgccttt
     2701 ttttgaactc ttagccatac gataagcatt ggcaatcact tctgaaattg tttctggatc
     2761 ttggatttct gcactgtatt ttgtgattgg ctggaacagt gctgcattat ccatgctttg
     2821 atgggtcaac ttcaatagat cgcttcgttt tacttgtccg gcaattgcta aaactgggtc
     2881 accttctgct gtcgctgtta ccaaacctgt agctaagtta ctagcaccag gaccacttgt
     2941 tgcgataact actcctggct ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc
     3001 attttgttcg tgacgagtca cgattaattc aggaccttga tcctctaatt cattaaatac
     3061 accatcgatt tttgcacctg gtataccgaa tacataatca acatgatgat taatcaaact
     3121 ttctactaca gatttcgaac cttgtttcat tttttcagcc attgaacatc gtccttttta
     3181 tgctttattt ttaattatta gttagaaata atcacaaata agtgatacag tttctaaacc
     3241 tgtttcattc aaaagtatag catgttttat ttcatttgtt tagccatatg ttt
//
LOCUS       DAZUKA010000217         3271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000217 DAZUKA010000000
VERSION     DAZUKA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.222"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8PN_002818"
     CDS             complement(451..1761)
                     /locus_tag="ACG8PN_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4862923.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3190)
                     /locus_tag="ACG8PN_002819"
                     /pseudo
     CDS             complement(1773..3190)
                     /locus_tag="ACG8PN_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1002 a          669 c          509 g         1091 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc cttttttctt tttttattgt atttgctgta acagcatttt
     3181 gtgaaagcat ttgaattcct cctacctctt ctagtaaacg ttttcaaaat tataactctt
     3241 tttttcttgt ttttatattc acaatgttgg c
//
LOCUS       DAZUKA010000218         3228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000218 DAZUKA010000000
VERSION     DAZUKA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.223"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8PN_002820"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8PN_002820"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4862924.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACG8PN_002821"
     CDS             complement(2262..2513)
                     /locus_tag="ACG8PN_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4862925.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..>3228
                     /gene="lexA"
                     /locus_tag="ACG8PN_002822"
     CDS             2660..>3228
                     /gene="lexA"
                     /locus_tag="ACG8PN_002822"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4862926.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIIL"
BASE COUNT          877 a          717 c          603 g         1031 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttaga
//
LOCUS       DAZUKA010000219         3199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000219 DAZUKA010000000
VERSION     DAZUKA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.224"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG8PN_002823"
     CDS             complement(139..1338)
                     /locus_tag="ACG8PN_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4862927.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG8PN_002824"
     CDS             complement(1468..2844)
                     /locus_tag="ACG8PN_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862928.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..>3199)
                     /locus_tag="ACG8PN_002825"
     CDS             complement(2844..>3199)
                     /locus_tag="ACG8PN_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4862929.1"
                     /translation="RLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALV
                     SDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELS
                     GKKLKKVNKMNLEVE"
BASE COUNT          979 a          612 c          454 g         1154 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaa
//
LOCUS       DAZUKA010000220         3191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000220 DAZUKA010000000
VERSION     DAZUKA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.225"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..1114)
                     /gene="murB"
                     /locus_tag="ACG8PN_002826"
     CDS             complement(179..1114)
                     /gene="murB"
                     /locus_tag="ACG8PN_002826"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4862930.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1187..2107)
                     /locus_tag="ACG8PN_002827"
     CDS             complement(1187..2107)
                     /locus_tag="ACG8PN_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4862931.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2220..2972
                     /locus_tag="ACG8PN_002828"
     CDS             2220..2972
                     /locus_tag="ACG8PN_002828"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4862932.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          905 a          634 c          551 g         1101 t
ORIGIN      
        1 ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa tttatgagga aatattctat
       61 attttttgtt ttattgtgtt aaaatgtaca agatttgcgc catttgcgta aacaaccaca
      121 aaaaagagca ggacggccac cgtcctgctc ttttacttat tttaagttcc attacttatt
      181 aattggctaa acttctgctt gtttctgata ttgcttgttt ttctccaatg atccgcactt
      241 ctgtttctaa agacacatca aatttttctt tgattacttc ttggatgtgc gcaatcaatt
      301 caatataatc tgtcgctgtc gcatgatcga tattgacgat gaatccagca tgtttttcag
      361 agacttgtgc accgccccat ttcaggcctt gcaatcctgc ttgttggatc agctgacctg
      421 tatagtgtcc tactgggcgc ttaaagacac ttccgcatga agggtattct aatggctgtt
      481 ttgattgacg caattcggtc aactcatcca ttcttgcttt gattgcttca taatcccctg
      541 tttgcaaact gaatcgagct tccaatacga tagcacgtaa ttcttggact ttactgtgac
      601 gataagaaaa taccatttcg tcttttgtca aagttttcaa tgttccatct tcaagcaaaa
      661 gatccacttc agcaaaaaca tctttgatct ctccgccata cgcacctgca ttcatataaa
      721 ccgctccacc gacacttccc gggatgccgc aagcaaattc aaatccagtc aatgattctg
      781 ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg accatcgttc
      841 ctttgacatg aatctgtttc atttcagtca gcatgatgac tacaccgcga atcccgccat
      901 cttgaacgat cagattgctt gcatttccta ataccatcca aggtatatca tggattcggc
      961 aataatcgat gatttgcttt acttcttctt gttttttcgg gaatgcaagg acatcagctg
     1021 gtccacctgt tttcgtaaac gtatagttca tcaatggttc atcaaacaat atagtaatcg
     1081 tggctaagct ttcaactatt tcttctttat tcactgagga ttttcctttc ttttctcaaa
     1141 cgtcctcatt attctatcac gcttaataca atcttgccag tcttgttcaa agttgatgac
     1201 ggtagtgatc tttaagcact tcctcacata attcatgact tcttacaact tctttttgtt
     1261 taagcaattc tgtttccgat ttacctaaac ggatctgaga aaagaaggcc tctaccattg
     1321 gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc aaattctttc actttttttc
     1381 catcagcatc ttgaatgaca agctgggtca gatcgtttac ttcataagta ccatttttac
     1441 ttgttacttg atatttttct atattagcgc cacttgctaa atccatcgtt aaaatagccg
     1501 tttgattttg tgtttctaat tgtaaaatgg catattccaa aaattccttt gtttctctta
     1561 tctggtattt tgttctcaaa atgggttcat ctaataaata cacagctgta tcaataagat
     1621 gtaaaaacaa atcataaagg acgaactctg tcgtttcttt tgccgctatt cgattttttt
     1681 gcaggtggat caaccgctta tctgggatac gtttcaattc ttctaccata ggagcaaagc
     1741 gccggttgaa accgatcatc aaaattattt ggttcttatc agctaattct tgcaattcac
     1801 gaacttcatt tagatggaca ctcagtggtt tgtcaatata aacatggata ccattttcta
     1861 gacatttttt ggctaattca taatgtactt ttgttgctgc gtggatcata catgcttcga
     1921 ttttttcttc taaaagctca tcgatcgttt catatagatg ttggaacccg tactcttttt
     1981 gaattctttc ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt gttccttgtt
     2041 ttttcgcata agttggtaaa tatgcttttt ctgcgatatt ccctacaccg attacaccaa
     2101 tcttcatcta ctttcctcct ggatcattta gttactttca tcttacacca tttttttcta
     2161 aaatgaggta caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt gtgcttttat
     2221 tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa aattttttag
     2281 aagtttttca tgagttagac gccgattttt tctgtctgca ggaaacgaaa ctccaagcag
     2341 gacaaattga tttagatctt ccaggttatt atcaatattg gaattacgcg gaacgaaaag
     2401 gctattccgg cacagcgatt tttgctaaaa aaccagcatt gaatgccact tacggaatgg
     2461 gtatcgatat ccatgataca gaaggccgtc tgattacttt ggaatattcc gatttctttc
     2521 ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa acgtttagat taccgattag
     2581 aatgggaaga agccttttat aattatttgg aaaatctaaa aaaacaaaaa ccagtcatcg
     2641 tgtgtgggga tctaaatgtc gctcatcaaa agattgattt gaaaaactgg aaaacgaatc
     2701 aaaaaaatgc tggattcact ccagaagaaa gagcggctct ttctcgactg ttggataacg
     2761 ggtttatcga tacattccgt tatttttatc caacccagga aggtgtctac tcttggtgga
     2821 actaccgatt caattcaagg aaaaacaatg ctggttggcg gatcgattac tttttgacga
     2881 gcaaagattt agagccagga ctagctgatg caaagatcca tacggacatt atgggcagtg
     2941 atcattgtcc agtagaatta gatttgaaat aaagaggttg tgaaaaagtc gtttagtttc
     3001 gagtattaag aaaaattttg tgaaaatggc tttccacatt ttgcataaat ttggcataat
     3061 accgagaaac tggcttttga acactgttta tcgagagatt gtgaaaaagc tgtcctgcat
     3121 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta
     3181 ttaccgcaga t
//
LOCUS       DAZUKA010000221         3162 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000221 DAZUKA010000000
VERSION     DAZUKA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.226"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACG8PN_002829"
     CDS             complement(63..2555)
                     /locus_tag="ACG8PN_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4862933.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACG8PN_002830"
     CDS             complement(2562..3029)
                     /locus_tag="ACG8PN_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4862934.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZUKA010000222         3047 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000222 DAZUKA010000000
VERSION     DAZUKA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.228"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(265..627)
                     /locus_tag="ACG8PN_002831"
     CDS             complement(265..627)
                     /locus_tag="ACG8PN_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862935.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(701..1081)
                     /locus_tag="ACG8PN_002832"
     CDS             complement(701..1081)
                     /locus_tag="ACG8PN_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4862936.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1163..2338
                     /locus_tag="ACG8PN_002833"
     CDS             1163..2338
                     /locus_tag="ACG8PN_002833"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4862937.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2415..2999)
                     /locus_tag="ACG8PN_002834"
     CDS             complement(2415..2999)
                     /locus_tag="ACG8PN_002834"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4862938.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          952 a          586 c          498 g         1011 t
ORIGIN      
        1 tgatataaca tccacttcag aatcaacgct gttaaattta tcatgtattt cttccttggt
       61 caactataaa ttaagcatta ctttgtgaaa tgatttttcg atcaattatt attattcgtt
      121 atacaaccgt tttcaacata ttttgaaaat aaaatgcact aaaaaactat ctttagcaga
      181 acatttcatt gtattttttg gaccaaacaa taaaaaaaga agtttgaaaa aaattcaaac
      241 ttctttgttc gcttaatctg tattttagaa gaaaggcact ccctctactt cgtaagttcc
      301 aagaacagag cgcatttgac ctggttcttc ccaaatatat ccttcgccta attcttctct
      361 ttgattcttt tgttcagttt caaagacaaa caacggaaca ttttcctctt taatatgtgt
      421 atttgtcaat tccatcaaac taatcttgct aacatcgata tgaactattt cttttaagaa
      481 attaagtata ttcgctaaac cagtctttcc ttcagaaagt tccgctaacg caaattccaa
      541 cttgtcatca attgaatgga ccaaaaattt tttagtacca tcctccaaat gcaacataat
      601 tgtaccagca actcttttgc ttgccaaaat ttccacctcg atcaacttgt tctatggctt
      661 acataaaaat tctagcacat tttcagaaaa aatacacttt ttatttctta ttttctttcc
      721 gttctagttc agataatgct tcttctatcc aaataggtaa ctgctccgca atagtggtaa
      781 atccgatctt acgattttta ttcgtgaaat acctttttct gcgatatgga acagctggat
      841 ttgttttttc taacgttctt aaagataaac tgattttttt ggaatattca tcaatgtcga
      901 tgatctgaac tgttactggc tgtccgactt ttaacaatga atggatattt ttcgtataac
      961 cggattgtac ttctgaaaca tgaataagtc cttgcgtctc gttatcaaga gaaacgaatg
     1021 cgccataagg ctgtatccct gtcacttttc cttcaattat ttgtccaatt ttataagtca
     1081 tctacttccc tctcttcgca tttatcatta tactgtaaat agctcaaaaa aagaagcgac
     1141 gaaaatgact ggaggtctta agatgatcga atttgataaa cagatttcaa gaaaagatac
     1201 agaaagtgta aaatgggacg caattgctga gacatatcaa atggatgact tacttccttt
     1261 gtgggtagca gatatggact tcttgtctcc cggtggggta gctaaagcgt ttagtgaata
     1321 catcaaacat ggaatctttg gttatgccac tctgtctgat aaactatatc aggctatcat
     1381 tcattgggaa cgccaacacc atgctgtgga gttgacgaaa gaaaatatcg tttttacaag
     1441 tggtgtgtta accagtttag ctgcagcagt acaaactttt acaaaaccag gcgattctat
     1501 tttgattcat gacccagttt atcctccatt ttcctctatt atagaaacaa atcaacgtca
     1561 catcgttcga agctctttgc tagaaaaaaa taatcatttc atcatggact tgtctgacat
     1621 ggagaaaaaa atcgaagaaa acaatgtaaa agcaatgatt ttatgcaatc ctcacaatcc
     1681 tggagggcgt gtttggagca aagaagaatt gaaacagtta agcgaattgt gtttaaaaca
     1741 tcaagtgatt ctgtttagtg acgagatcca tcaagattta actttattcg atcatacatt
     1801 tacttccatg ctaacgattg atcctaattt agatgatcta ctagttgctt ttacttccgc
     1861 aaccaagact ttcaatctag ctgcaatcaa aaattcaatg gtatttatca aaaatccaga
     1921 gatgaaagct gattttgaaa aacatttagt gatgaaccaa cagcacgaaa tcaacacttt
     1981 tggactgatt ggaacccaag cggcttatga gacgggtgga gaatggttaa gccaactgat
     2041 tccttatcta gaaaaaaatg ttcaaacagt taatcagttt tttgaacagt atcttccgaa
     2101 agtgagaata atgaaccctg aaggaactta cttaatgtgg ttagatttta ccgcctatat
     2161 ttcagacgac caagaattgg agaacactct tgttcggaaa ggaaaagtcg tattaaatcc
     2221 tggcatcacg tttggtcctt ccggtcatgg gcatatgcgc ttgaatattg cttgtccaga
     2281 agaaacttta ctggaaggac ttaatcgaat caagtatgct ttagagacaa tcgattaaat
     2341 catttattca aaataaaagg acgagacctt actaggtttc gtcctttttc ataattactc
     2401 cgtaccatac caaactattc tgaaatttca attgtttcaa tcactacatc atgtacaggt
     2461 cggtcttgtg gaccacgttg tacatttgcg atttcatcta ctgtgtccat cccttctagt
     2521 acatgtccaa agactgtatg tcggaaatct agccaaggag ttccgccacc tttatacgct
     2581 tcaatgattt ctgatgggaa tccagcacct tctagttgtc caagcatatt agctggcaca
     2641 tttgtgttat ttacaataaa gaattggctt ccgtttgtgt ttggacctga atttgccata
     2701 gataaagctc cgcgaaggtt gaacagttca cgactgaatt catcttcaaa actttcgcca
     2761 taaatacttt cgccacccat tcctgttcca gttggatcgc cgccttggat catgaaatca
     2821 ggaataacgc ggtgaaaaat cacgccatca taataacctt ttttggaaag ttcaatgaaa
     2881 ttttgaaccg tcttcggtgc tagttcaggg aataattgaa cagtaatgtc accacggttt
     2941 gttttgataa cagcttttgg tcctttttca ctttttaaat ctagttgtgg gaatgccata
     3001 ttgtttcctc ctaatatttg catacttcat aataataata acataat
//
LOCUS       DAZUKA010000223         3026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000223 DAZUKA010000000
VERSION     DAZUKA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.229"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1858)
                     /locus_tag="ACG8PN_002835"
     CDS             complement(92..1858)
                     /locus_tag="ACG8PN_002835"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862939.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1818..>3026)
                     /locus_tag="ACG8PN_002836"
     CDS             complement(1818..>3026)
                     /locus_tag="ACG8PN_002836"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862940.1"
                     /translation="IQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFKALNKAIND
                     YAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLS
                     GIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVS
                     FSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLN
                     HYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFP
                     EREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQH
                     LMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENAN
                     DWRLDNEKFL"
BASE COUNT          987 a          565 c          428 g         1046 t
ORIGIN      
        1 aaaattagat gctgtttaat aaaattattc tttttgaaaa agaattataa ctatatccca
       61 aaaattgtag gttaatttaa agcctatttc atcatcgtga ttgagcttct ttttcataga
      121 cgaatttctc agatagttta gttttcttta tgagattttt gtatatttct gaccttcgat
      181 acaactcatt atgagtacct tgatcaacta gttttccttg atccatcact acaattttat
      241 ttaccttttc aatagacttc aagcgatgcg atataattag tactgtctta tcttgtatta
      301 aacgatttaa actttcctga atacgttttt cattttccgc atctaaactg gcagtaattt
      361 catctaaaat taaaattggc gcatttttca aaaaagcccg tgctatggat aggcgttgtc
      421 gctctcctcc agaaagtgaa cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta
      481 gtttttcaat aaagtcttcg cagttagcta aacgagctgc ttttttcact tcctcatctg
      541 acgcttgact atttccaata cgtatatttt ccaaaatact tgtattaaac aaagttacct
      601 cttgaaaaac aatagcgatt ttgctaaaca aagaagctgg agatacacgt ttaatatcat
      661 acccatcaat taaaatacag ccttcatcat aatcatacag tctagacact aatttcaaga
      721 tactagtttt cccggaacca ctagcaccta ctaacgcagt cacttctcct tgtttagccg
      781 taaaagagat atgatctaaa attggggtat tgttatcata tgagaaagag acatctcgca
      841 actcaatgtc aaaagatttc agaggactat ctgatccctc ttggatactg gtttccttca
      901 tagctctaat tcgctgtatt ttaggagcta aataaaatat ttcaagcact gcttctttca
      961 tagaatcgaa ggaatccttt atttttattg cggctagtaa gtaaccaacg acgtagagta
     1021 tagtcacttc acccgttaac agtaagtgaa cacctactag aatgactact gctaaactaa
     1081 catatgagaa aatagaggat aaggccatga ctgaaaaagt acttaattct acctttaaat
     1141 gaatcctttc actctcttcc atttttttga aaagtctgtc ttgaactttt ttagatagat
     1201 taaagctatt aatctcttgc tgcaattcta tagtttcttg aaattcttcc gagttctctc
     1261 tcaaagtatc gtaatattta gtattggctt tcgtttggga tttctttgat aacaagatta
     1321 agacaaaact aaataacgtt ggcaaaataa cagctaatcc cattttgaca ttaccaacca
     1381 aaagcatcac tgaaataaaa gggaaaaaca gagccatacc gccggcttta ggtattgcat
     1441 gactcatcgc atgctcaata ccttcaacgt cagacatgat agtttgagat aaatctgata
     1501 aattatgttt tgaaaaatag gataatggta aatttgacaa atcgtctgca atttgcactc
     1561 ttaaatgcgc actttcttta taagtactat tatataagtt ttcatactcc ctatctaaca
     1621 accaatacat gaaaagcaaa gtaacaaacg atacggcaaa ataaagccaa cggggctttg
     1681 tattttctaa aactaattcg tctatcagca tcattataat gaatgctggt accatattaa
     1741 tgcagtatac aaaaaaacta gaaatagaag ctttagttaa agcttctgcg ccctgatcac
     1801 ttaaagcaaa tttgttttta taaaaacttt tcattgtcaa gcctccaatc atttgcattc
     1861 tcataattag tgaccaattg tttatacact tgatgtttag ccattaattc attatgattt
     1921 ccacgttcta ctattttccc ttctgacaag acaagaattt catctacaga tttaatgctg
     1981 gataatcgat gagctatcat aataactgtt ttttctttca tcagatgttg aaaagccttt
     2041 tgtagttcat actcattatc tggatcaata gatgcactgg cttcatccat aataacaatc
     2101 tttgcatctt ttaaaattgc tctggcaata gctatacgtt gtttttctcc tcctgacaga
     2161 taaactcctt tactaccaat taaggtatgc tctctttcgg gaaatttttc taaaatcgat
     2221 tcgcaaccgg ctaatcttag cgcgtcaaat actttacttg ctggcgcttg aggatttgct
     2281 atagaaacgt tatcgtaaat actctgattg aatagtttcg tatcttgaaa gacaaaagca
     2341 attgtgttaa taattgtgtc ttttgaatag tgatttaaag gcttaccacc aattttaatc
     2401 tgtcccttat ccacattgta gaaacctgaa attagtttag ctatcgtact ctttccgctt
     2461 cctgattttc ctactaatgc ataaattctt ttttgttcta aaactaagtt gaaattttct
     2521 aaaacacact cttctttgta tgaaaagctg acattttcaa atgcaatgtc acaattttta
     2581 atctgtgtct cgttcccata ttttagttgg tcttgcttca tttctaaata aagggagtct
     2641 agtgtttcga ctgcatatct tgcttcaaac aagtgcatac ttacatacat tattctcatg
     2701 aaagaaacaa aaataattcc acttaaaaaa aacaacatca acaagtcaac agctaatatc
     2761 ttgggagtgg taagagaagt tataaaaaag gttataggaa ctatcaaaat gcaaactatg
     2821 ccaaaaaata gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga atacttatat
     2881 gcaaacttag cataatcgtt aattgcttta ttcaaggctt taaatgatgt tactttagca
     2941 ccaaatatct ttataacttg aattccgcgg atatattcta ccgtttcaga gcttaacaca
     3001 tctaacgatt cttgatattt ttgaat
//
LOCUS       DAZUKA010000224         3010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000224 DAZUKA010000000
VERSION     DAZUKA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.230"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..652
                     /locus_tag="ACG8PN_002837"
     CDS             65..652
                     /locus_tag="ACG8PN_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862941.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            652..1551
                     /locus_tag="ACG8PN_002838"
     CDS             652..1551
                     /locus_tag="ACG8PN_002838"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4862942.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1551..2465
                     /locus_tag="ACG8PN_002839"
     CDS             1551..2465
                     /locus_tag="ACG8PN_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862943.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            2517..2732
                     /locus_tag="ACG8PN_002840"
     CDS             2517..2732
                     /locus_tag="ACG8PN_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862944.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         1166 a          370 c          637 g          837 t
ORIGIN      
        1 ctttatatga tatatagttc cttgtcagag gaagagataa ccagcaatat tggagggata
       61 ataagtggaa aacaataatg atattagaca ttttagcatt ggggaaagag ttacaacagt
      121 aagtgaatta tcaaaggatt atagagataa agagggaacg attatttctc ttaataagaa
      181 tcgaagaggc gtttggcaat atgttgttga tttaggtgat ggcgttacag ttgagtgtaa
      241 agatagtcaa ctgatcagaa ataatggcag tcattccatt gaaaaagcaa ttcttgtata
      301 cccaaaagta tcaagtaaga tcatgaatga ctggttgctt ggaaaatttg gtgagtacaa
      361 taaaaaagaa atcgcagaag tgatcggcaa atcttatcca acagtattag ggtatctaaa
      421 aaagataacg agtaaagaag caaaaattga ggatgtttta acagtaagag agcttcaatc
      481 attaatcgag tatcggaata aacaaataac aaatcctgag taccttagaa aagaagatgg
      541 tctattgaaa caattggaac aagtaaataa aatccggaag atgcaaaagg tagctccttt
      601 aagccttgat acctttaaaa aaatctattt gaaagagatc aaaaaagact aatgtggact
      661 gtaactttac cgttccaacc tgtatctgca ccaagaccaa gtgcaagaag gaatgctgct
      721 gatgaaacta atgacgtagc agagaaaagg atttcaacct actatgataa aaagtatctt
      781 gattatttga aaaaaataaa gagctttatc gaagatcaag atttattaga tgatgaattt
      841 tactcgattg taaatgatcc tatgggcgca attatggaag tggactttta ctaccagata
      901 ccaaaaagct ataaaaaagt ctataacatt atgaagacaa cagcgccaga tttagataat
      961 ttagtaaaaa gtgctatgga tggtattttt aacatctcag ctatacgaga tagccatatc
     1021 acaggcttaa ttgcttttaa atataatgtg gcgaacgaag gaaaaacagt tgtgaggatc
     1081 aaacgatatt cagaatttga gtgggatact tctgaaggat tgaacggaga tatatgggaa
     1141 gctacatttg attttaaacc agtagcaacg ccaagaccaa agtctaaatt tagaggaaat
     1201 gggattatca cttactatcc aaaagagtac aacgactatt tggaaaatat caagaatacg
     1261 cttaaagaga aagatttggt aaatgatcaa ctgaaaaagg taatgaatgc tcctatgggg
     1321 gttatagctc agatagatta tttttaccaa gtgagaaaag ataagaaaaa gcttgattca
     1381 ctgatgcgta cttcacagcc ggatattgat aatttagtca aaggaacatt ggacggaatt
     1441 tttaacaagg aaataggcat caaagatagc agggttgtag ggttaattgc ttttaaatac
     1501 aatacttttg aaaagtcgaa aacaaacgtg agactacagg agatgggctg atgaataata
     1561 atgaaaaaat tcttcatgta cttgatagct ttgaaataat tcaagaagaa ctaaaaaaat
     1621 atcgggatgt tttggaacaa agatatgatt ttgtcaatca gcaaaagagt aatcacatgg
     1681 atttcattct caatatgaat gacttgaaga agaaattagt tgaacgaaaa gaacaagaaa
     1741 agttgatcaa agcttacttt gaactcggtg aaaaagaagt taaaaatgca atggaactga
     1801 atgaagagcg cagggtttta gatcagctgt tagaacaatt attggtgatg tttcaaaaag
     1861 gacgaataga tgaggattta attgaagaag gattgagaaa atatccttca aactcaggta
     1921 tcggaatagt gctgaaagcg attgatgaag aagaaattga agctttcata ccgccagagg
     1981 attttgaatc ggcaatggaa tatataaaat attatagcca agggatcact gcttttagag
     2041 agtttgatcc tgaagatgtt attcatgact tgaacaacct aaaagaatgg tgtgaaagtt
     2101 atggagtcga tgattccgga ttggactact tgatcagtat tatggaaatc gaagaagaga
     2161 tgccggataa gcctgatcca acagatattt tagaattgat ccatgaagct agaaatccaa
     2221 ttgcctatat ttctagagga tatactgtat tggaatacta taagccttat atatcagcga
     2281 tgaaccattt aagacgtgta ttaagggaaa aacgtgaata ccgctcagtg ttgaatgcaa
     2341 cgaatagatt agaaaaagct gtttcagagc tagatgctta ttatcgtgaa cattacctgc
     2401 aagcaggtgg tatgccaaga aatacgaaag caaatatcag taggtatata aaaaaagctg
     2461 agtaggcgga gttagcccat aaaatggcaa aaggtaacct gaaaggggca ttttgaatga
     2521 aaagggatgt ttttgaattg tacgtattag aacgtctaga agacggcatg atggcacaag
     2581 agataattga tgaaattgat gatatgatca gcagatttgg tcaattggag ttgaatgatg
     2641 gaaacaaaaa ttcggtagtg ctaaaacaat tttggttgaa caatttttcg tataaggatt
     2701 ttatcaaaga aaaggcaaat acattgtact gactaaattc acgaggagca gtgatgagag
     2761 cttattgcta agagcagtta ggctcttttt tgtttgtgta aaattatttg ttttataata
     2821 cacatttata cacaaaaaaa tattgacaga tagtacacaa atgtggtagg atatatttgt
     2881 aagcaagtta gattaacaag ttaaaggaag agcagttatg aaaaaagaag aactaataga
     2941 tatatttcaa attgttgaac gagcaaataa tatgggaatt atgttttttg atcgtatttc
     3001 tttaaagatg
//
LOCUS       DAZUKA010000225         2980 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000225 DAZUKA010000000
VERSION     DAZUKA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.231"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..949
                     /locus_tag="ACG8PN_002841"
     CDS             182..949
                     /locus_tag="ACG8PN_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4862945.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            939..>2980
                     /locus_tag="ACG8PN_002842"
     CDS             939..>2980
                     /locus_tag="ACG8PN_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4862946.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          952 a          481 c          603 g          944 t
ORIGIN      
        1 aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg ctgaataaaa
       61 taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata agagcaaatt
      121 attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag gaggagaaga
      181 attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca aactttctcc
      241 cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg ttgggattat
      301 gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga tcaatttgcc
      361 tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa acagtgagct
      421 cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt tgatcgatac
      481 cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga ctaaagagga
      541 aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac tgctcaaacg
      601 gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta gagcacttgt
      661 tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact cgaagtcggc
      721 tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa cgatactgtt
      781 agtcacccat gatccatata cagcaagcta ctgcgataga attttattta ttaaagatgg
      841 tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg agaaagtaat
      901 agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa ctagccgtta
      961 aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg actttatccg
     1021 taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc agaagagcaa
     1081 gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt actgttgttt
     1141 tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa aaggaaatag
     1201 gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat gtgttagaaa
     1261 tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt attttttcca
     1321 aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt ccatttttta
     1381 tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt ttagcagtat
     1441 ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt ggagaaaaag
     1501 aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc gcagtcatcg
     1561 ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa atcacttcat
     1621 ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt ttaatttttt
     1681 tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta tttttgatca
     1741 tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg ggtaacacgc
     1801 aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt ctcggaattt
     1861 ctttaatgac aattggcggc acggctagta tctctactat tttatcctac caggagaaag
     1921 tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc aagccgattc
     1981 tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa gtcattggga
     2041 gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg atcacggaaa
     2101 cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct ttgaaatcac
     2161 ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt tcattatatg
     2221 gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg cttccaagtg
     2281 tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat gataccgttt
     2341 ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca ggtggaaata
     2401 atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc aatgctgttt
     2461 attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca aagaaacttc
     2521 cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg aatttctctt
     2581 ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt tttgtcacac
     2641 tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc cggcaattag
     2701 cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg attccacaaa
     2761 gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca cccatgatat
     2821 tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata aaaactgccg
     2881 attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt ttgctttatg
     2941 tcattacgag tcagttatat tgtcggatta tcgaggaatg
//
LOCUS       DAZUKA010000226         2976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000226 DAZUKA010000000
VERSION     DAZUKA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.232"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..686
                     /locus_tag="ACG8PN_002843"
     CDS             255..686
                     /locus_tag="ACG8PN_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4862947.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            693..926
                     /locus_tag="ACG8PN_002844"
     CDS             693..926
                     /locus_tag="ACG8PN_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4862948.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            1350..1550
                     /locus_tag="ACG8PN_002845"
     CDS             1350..1550
                     /locus_tag="ACG8PN_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4862949.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            1634..2827
                     /locus_tag="ACG8PN_002846"
     CDS             1634..2827
                     /locus_tag="ACG8PN_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4862950.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
BASE COUNT          833 a          697 c          797 g          649 t
ORIGIN      
        1 cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag catagcaaag
       61 cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct tttgcggccc
      121 ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta gcgggtagct
      181 ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt cccccacaga
      241 aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc atgttcgatg
      301 ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa atgaagcgcc
      361 gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac aagctctgta
      421 cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat gacctacata
      481 tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag cagagtattt
      541 taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt gtgggaatgt
      601 cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg aaacagctaa
      661 aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac aagggcattg
      721 agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga gccgtagagc
      781 gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg ctctatgacg
      841 aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg gaaatcaagc
      901 tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa cggcttaccc
      961 tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata tgcccgcaag
     1021 ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag ccggacggct
     1081 ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca cgccgcaatg
     1141 tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc ccttatagcc
     1201 acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga accacgcggg
     1261 ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa atcacacaaa
     1321 cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt tgggaaaaat
     1381 acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac aaattgcgta
     1441 agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac cgcatccaga
     1501 tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa tagtgaaaaa
     1561 gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc gtcatggaaa
     1621 ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt tgcggtctgg
     1681 agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct ttggaaaacc
     1741 gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag gtaaacggga
     1801 ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg acgggataga
     1861 caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca ggcaccgggg
     1921 caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc tggaacaaga
     1981 tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg aatgggcgct
     2041 tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc gttccctgaa
     2101 agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt ttgacttcca
     2161 gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac cagcacagga
     2221 agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt atgacgcaat
     2281 cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga cggacaaaga
     2341 tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa ataccggcgt
     2401 cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc ccatgaatga
     2461 ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa agccgttcat
     2521 tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca tgacggcggt
     2581 agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc atgaagaagc
     2641 gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc ggttagccaa
     2701 tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata tcaccatgac
     2761 gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg aacggctggc
     2821 agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg aaatggtgag
     2881 aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa agcccggaaa
     2941 cacgggctat accgacattc aagaacttct aaaaag
//
LOCUS       DAZUKA010000227         2925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000227 DAZUKA010000000
VERSION     DAZUKA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.233"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..204
                     /locus_tag="ACG8PN_002847"
     CDS             <1..204
                     /locus_tag="ACG8PN_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862951.1"
                     /translation="YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSV
                     SEEKNLDTTFIADLYKENGVELK"
     gene            201..1505
                     /locus_tag="ACG8PN_002848"
     CDS             201..1505
                     /locus_tag="ACG8PN_002848"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4862952.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1613..2470
                     /locus_tag="ACG8PN_002849"
     CDS             1613..2470
                     /locus_tag="ACG8PN_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4862953.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(2582..2902)
                     /locus_tag="ACG8PN_002850"
     CDS             complement(2582..2902)
                     /locus_tag="ACG8PN_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4862954.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT          965 a          549 c          593 g          818 t
ORIGIN      
        1 tacaaaaaag taaataaatc ttttgaatat gaagatgaaa aattgaaaaa aagacaagaa
       61 ttacttgaag aagtgtatac ggaacgtttt ggaacaaaag aagagagaga aacggtccgt
      121 ttttattctg tctccgaaga gaaaaatcta gatacgacat tcattgcaga tttgtataaa
      181 gaaaatgggg tggaactgaa atgatctcaa tcacccatac aggtaattta ttcttagata
      241 cttgtttgtc tattatgtat ttttttctcg tttcttatcc gattttaggc ggatttgtat
      301 ggtttattgg tgtctggtgt tatgtgtttt tatacaaaca taagcaaaaa gaatgggtag
      361 atgttccctt gagtgtcgaa ccgttcatca cgatcatggt gccagcccat aatgaagaaa
      421 ttgtgatcga atatacaatc gagtatttga tgacgaaatt gaattatcac aactatgagg
      481 tattagtgac agacgatggt tcaacggacc aaacaccaga aatattagct agactgatga
      541 aaaaatacgc taatcttcgt gtggttcgga tcgaaaaaaa taaaggaaaa gcccacgcgt
      601 ttaatatcgg acttgccttt gcaaaaggga aattgatttt aagcaatgat gcagacacgg
      661 taccggagcc agatgcactg attaggtatg tgaattattt cattcgccct ggtgcaagac
      721 atattgctgc ggtcactgca aatatggatg tccaaaatcg taccaaattg attgcaaaat
      781 ctcaaacggt tgaattctca agtatcgtgg gcatcattaa acgaacacag tcagctgttt
      841 tcggtggtct ttatgcgtac agtggagcaa atacgatgta tcgaaaagaa gccttgatcg
      901 atgtgggcgg attcagacaa gatcgagcga cagaagacat cagtattgct tgggatcatc
      961 agttaaatga ctggttatct gtttttgctc ctgagattat ctttttcatg gaagtacctg
     1021 tgacgttgaa gatgctatat cgacacagaa aacgttgggc aaaaggtgga acagaagtct
     1081 ggctgacgaa tttcaaaaaa gtgatgctcc atccttttaa acatattgga agaacgatca
     1141 tcttcattga tcaaacattg agtattgtct ggtcaatatt cttttgtatt tccgtagttc
     1201 tttttgctgg attaatcggg cattatgtat atcaaggaaa ctacgaacag atttatatta
     1261 cttttacatt cagttttgtg ttcatctgct ttgaaatggt cgcaggattt ttccagttgc
     1321 tagcttcgct gattgtagat gatcgacgaa gaaaattgaa ataccttctt ttcgcaccac
     1381 tatatatgct tttgttctgg atagtgaacg cgataacgat cgtgacgact ttcattccag
     1441 cagtcaaaac gattttaggt tatggaagcg ggacatggaa aagcccggaa cgtacaaaaa
     1501 aataaaagat gcaccgtttt ttagacaaac agcctgttgt gtttatggtt cttccaaaca
     1561 tttttgctta taataagaaa ttagttaaaa agtagtaagg atgggaaaag tgatgaaaaa
     1621 aagaatgaag ccgttaatct ggccaattgc tgtttccagt attttcttat tgggacagac
     1681 agcttttgcg gaaacttctg aacctgctca aacagaacct tcaacaacca taagctcttc
     1741 tggtgctgaa aacggtgcct cggaaacaga aatgacttcc aatgtaccga cagataattc
     1801 aggagaaacg atttcatctt cagaagatac atcaataaat agttctacct cttctgacca
     1861 aatcgatcat ttgcaagact tggaaaataa aagaaaagaa ctcgaagaag cattagaagc
     1921 tgcaaaaaac aaattgaaag aagaccaagc agcaatcgat caagatttag ctgatcttaa
     1981 aaataaagtg ggaaatgacc gtgcacaact tgagaaaaag cttgccgaag cgaaagcaaa
     2041 agcggaagct gtccaaacag atcttgacgg gaagatccaa caagcaaaag aattcaatca
     2101 gcaattaaat caggttgctc aaacagcttc tggaaagcta agtcaaactc aagcggatat
     2161 cacagcggcc agtcaagcaa tccacggaaa gatcagcgaa gcacaaacag ttgctaatga
     2221 ttttgaagca aaaaaagctg acttagcagc tagtttccaa cgaataaaag atattttgca
     2281 ttcggcagga gaaaaatcag tgacctctgg aagcacgaca gaaacagttc agcaaacgac
     2341 agagccatcc aaatcaacaa gtcagacttt gccaaaaaca aatgaaaaaa cagcttctgt
     2401 cagttattcc ttagcagggg cgggtgtgtt agtcgcagca gcttacggaa tcatccgcaa
     2461 aaagaaataa aaaagagagg atgggaaggt agagtacctc gaagttagac tgaaaaatct
     2521 aattcttgag tgccactaca tgtcccatct ctctttagtt attaaaacag atgtccccta
     2581 cttatctcgt ggagacagct gttccactac atgtgaccat cagcatgcca ttgtcgctgc
     2641 ctaatacttc atagtccatt tttacgccaa tgatcgcatc agctcctaat gcttttgcac
     2701 gttcggccat ttcggcaagt gcttcttctc ttgcacccat tagttcgttt tcatagcttt
     2761 ttgagcggcc gccaaagata tttcgcaacc ccgcctccaa gtctttaaat gcatggatcc
     2821 cagtaatgac ctcaccaaaa acgatccctt tataagctac tacggctttt ccctctacac
     2881 tgttcgttgt tgttgtaatc attcgatcac tcctctcttt taatt
//
LOCUS       DAZUKA010000228         2924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000228 DAZUKA010000000
VERSION     DAZUKA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.234"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..616)
                     /locus_tag="ACG8PN_002851"
     CDS             complement(74..616)
                     /locus_tag="ACG8PN_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4862955.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="ACG8PN_002852"
     CDS             751..1140
                     /locus_tag="ACG8PN_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862956.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="ACG8PN_002853"
     CDS             1144..1908
                     /locus_tag="ACG8PN_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4862957.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2037..>2924
                     /locus_tag="ACG8PN_002854"
     CDS             2037..>2924
                     /locus_tag="ACG8PN_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4862958.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKG"
BASE COUNT          943 a          490 c          600 g          891 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaataca actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggg
//
LOCUS       DAZUKA010000229         2898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000229 DAZUKA010000000
VERSION     DAZUKA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.235"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..2136
                     /locus_tag="ACG8PN_002855"
     CDS             76..2136
                     /locus_tag="ACG8PN_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4862959.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2219..2788
                     /locus_tag="ACG8PN_002856"
     CDS             2219..2788
                     /locus_tag="ACG8PN_002856"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4862960.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1042 a          521 c          560 g          775 t
ORIGIN      
        1 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
       61 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
      121 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
      181 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
      241 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
      301 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
      361 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
      421 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
      481 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
      541 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
      601 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
      661 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
      721 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
      781 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
      841 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
      901 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
      961 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     1021 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     1081 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     1141 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     1201 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     1261 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     1321 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     1381 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     1441 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     1501 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     1561 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     1621 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     1681 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     1741 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     1801 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     1861 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     1921 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     1981 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     2041 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     2101 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     2161 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     2221 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     2281 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     2341 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     2401 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     2461 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     2521 gcaagtgtat ccggtttttc attggttagc agtgaaatca gattaccaag gaaaagggct
     2581 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     2641 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     2701 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     2761 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     2821 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     2881 taatggcatg ccatacat
//
LOCUS       DAZUKA010000230         2894 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000230 DAZUKA010000000
VERSION     DAZUKA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.236"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1338
                     /locus_tag="ACG8PN_002857"
     CDS             <1..1338
                     /locus_tag="ACG8PN_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862961.1"
                     /translation="KIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYA
                     YGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVV
                     VNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDIT
                     TYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQ
                     FPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQ
                     SKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAA
                     DNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEI
                     TTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1325..>2894
                     /locus_tag="ACG8PN_002858"
     CDS             1325..>2894
                     /locus_tag="ACG8PN_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4862962.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTII"
BASE COUNT          987 a          435 c          497 g          975 t
ORIGIN      
        1 aagatagatg ctgctttttt atttagttgt ttatatactt ttgcaactta tcgaacagtt
       61 gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat cccagctgtt
      121 ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc tttatctgtc
      181 agtataagtc tacttgtgta tactcatgtc ttaactacac tattgtgtag tgcattgatt
      241 gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga tataaaattt
      301 gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag ctatttttgg
      361 tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa aacagatttg
      421 cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa cgatattaca
      481 acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt catactgaaa
      541 gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg ggtatgcgta
      601 actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact ccagtttcct
      661 tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct tgtttttatg
      721 aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt ggtgtttcta
      781 ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc aaaacacggt
      841 catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat gggaggatta
      901 tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa acatgaagta
      961 aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa cattacttac
     1021 acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta tttaggtaca
     1081 caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg cttagtgaat
     1141 gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac tccaactgct
     1201 ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc aagttttaaa
     1261 cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga gataaacaag
     1321 aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag ttagtagtat
     1381 atattctttc atctttttca aaaatacaca gagtatttat accactggag atctttcttt
     1441 tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac
     1501 attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc
     1561 tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt
     1621 attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa
     1681 aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga cagtagacat
     1741 ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc ctgtattatt
     1801 ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa agctagcatt
     1861 gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta cagcattaat
     1921 tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag attttattgc
     1981 tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga gtgctttttg
     2041 gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg tgacaaattt
     2101 atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca acgacttaac
     2161 tacttactca atgggactag ttggtctgtt atctttatgt attcccctga ttttatttaa
     2221 aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt ggctagctac
     2281 aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt tacaatttcc
     2341 ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga tgatattttt
     2401 caaaaataga agatacaata agacaagaga actattttat ttatttatga ctgttttgtt
     2461 attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa tttctcagcc
     2521 gggacatatt gttgtaaaca aagaaacagt agaggcgtat accactaaag gaatggatta
     2581 ttttttatat gattattctc ctatcgcttc attggagaat aaggagcata taataaaaca
     2641 cgaagcaaag attggcagtc aatgggtaaa ccttccttat aaaacaactg gtagttctat
     2701 catatataga gtaactaatg agaagagcca agtaatcaca cttcctgtat atgcttattt
     2761 aggaacgaat gtagaaataa atcatcaacc tgtaaaggag caggtgaatg ctaatggatt
     2821 ggtacaaata agcggagtca aaggagttgc tgatattaaa gtaagcacaa gttatacagt
     2881 aacgactatt attt
//
LOCUS       DAZUKA010000231         2762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000231 DAZUKA010000000
VERSION     DAZUKA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.237"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..549
                     /locus_tag="ACG8PN_002859"
     CDS             103..549
                     /locus_tag="ACG8PN_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4862963.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            822..2174
                     /locus_tag="ACG8PN_002860"
     CDS             822..2174
                     /locus_tag="ACG8PN_002860"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4862964.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2345..2632
                     /locus_tag="ACG8PN_002861"
     CDS             2345..2632
                     /locus_tag="ACG8PN_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4862965.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          974 a          451 c          545 g          792 t
ORIGIN      
        1 ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt taaaatgtaa
       61 aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa taagttagtc
      121 gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca acggtacgcg
      181 gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat ttataaagca
      241 cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct tcgcttattt
      301 acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg atttgatttc
      361 agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt tgattttcat
      421 tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt tgagattaat
      481 gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc aaaaaaggcg
      541 caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg tgtaaaaaaa
      601 acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa agtgaatatt
      661 attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat gaaactacaa
      721 acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt ctgttatagt
      781 agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta gtagtaattg
      841 gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat ccagaagcag
      901 aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt atcgcacttt
      961 atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct gaagaattat
     1021 cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat acagaaaata
     1081 aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct tatgataaat
     1141 tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc gatgcgaaaa
     1201 atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa gcgaaagaag
     1261 caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta gaagcttttg
     1321 tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg aataaatatc
     1381 tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt gttactcttg
     1441 cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg aaacaagtag
     1501 ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc ttccgtccaa
     1561 atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc aaagtgaatg
     1621 aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca gtagtcaact
     1681 acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt cgtcaaggtt
     1741 tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact caaggaactt
     1801 caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa gaaagcgcaa
     1861 ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt ccagagttca
     1921 tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg aatcgtatcg
     1981 taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc atgtctttag
     2041 ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc ttccaaccac
     2101 attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta ggcgaaatcg
     2161 aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct ctgtcttttt
     2221 ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa gcagcaaaga
     2281 taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa aggtgagaaa
     2341 gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt ataaaagtgc
     2401 ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct ctcagttgaa
     2461 tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata atgtttctca
     2521 tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat tgaatacaga
     2581 gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt aaagacagtt
     2641 tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta ccgatgactg
     2701 tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca accccttttt
     2761 tt
//
LOCUS       DAZUKA010000232         2684 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000232 DAZUKA010000000
VERSION     DAZUKA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.238"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(939..1457)
                     /locus_tag="ACG8PN_002862"
     CDS             complement(939..1457)
                     /locus_tag="ACG8PN_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4862966.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1824..2072
                     /locus_tag="ACG8PN_002863"
     CDS             1824..2072
                     /locus_tag="ACG8PN_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862967.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            2575..>2684
                     /locus_tag="ACG8PN_002864"
     CDS             2575..>2684
                     /locus_tag="ACG8PN_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4862968.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          890 a          485 c          394 g          915 t
ORIGIN      
        1 gcaaatttac ccctatacta ctagtttgta agttagatta ataaaaatac taaagttatt
       61 tgttctttta ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag
      121 aataaaaata acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt
      181 tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag
      241 aaatcgttac tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact
      301 tctactattt gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc
      361 ccgtatagaa tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca
      421 taccctttga tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata
      481 ttaacaaagg ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca
      541 attcttccta acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg
      601 gttgttgaga ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc
      661 ccaaaaataa aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc
      721 accataatct cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat
      781 acaatctaac gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata
      841 aaaaccggca gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga
      901 ggtctccttt ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat
      961 tttcttatct ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta
     1021 tatacttccc atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg
     1081 aatagatctt catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg
     1141 agaatttcct ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat
     1201 aactctccta actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt
     1261 ctttcatcat tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt
     1321 tcagcaggtg ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt
     1381 tcaaattcta aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta
     1441 tttttatatg ttctcattat attacctcca taaattccta attgtctcct ttaattcatc
     1501 tataatttca tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact
     1561 gtctaactca tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg
     1621 atcgtcagca aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt
     1681 ttgcatatca ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa
     1741 aaactaccac tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa
     1801 atccattgtt aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc
     1861 atcgctcttt cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg
     1921 acacgttcct tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag
     1981 aaaatttaat tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag
     2041 gtcaagattt acctgaagat catccttttt aggaaattga agctttttat gttgcacaat
     2101 gcatttataa tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga
     2161 aacagataca cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg
     2221 ttaatcttcc gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta
     2281 aggttggcaa aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt
     2341 tggttgacac ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt
     2401 acctaaaaag tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat
     2461 ttcaacttat atgttttagt gaactacccc cacttagctt ggataggcta caattaacta
     2521 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
     2581 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
     2641 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taac
//
LOCUS       DAZUKA010000233         2675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000233 DAZUKA010000000
VERSION     DAZUKA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.239"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACG8PN_002865"
     CDS             699..785
                     /locus_tag="ACG8PN_002865"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4862969.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACG8PN_002866"
     CDS             complement(1087..1311)
                     /locus_tag="ACG8PN_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862970.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACG8PN_002867"
     CDS             1677..1961
                     /locus_tag="ACG8PN_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862971.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACG8PN_002868"
     CDS             1964..2380
                     /locus_tag="ACG8PN_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862972.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACG8PN_002869"
     CDS             complement(2517..>2675)
                     /locus_tag="ACG8PN_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4862973.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAZUKA010000234         2671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000234 DAZUKA010000000
VERSION     DAZUKA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.240"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..564
                     /locus_tag="ACG8PN_002870"
     CDS             <1..564
                     /locus_tag="ACG8PN_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4862974.1"
                     /translation="LKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICI
                     LPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASF
                     KIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNS
                     SRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            537..1214
                     /locus_tag="ACG8PN_002871"
     CDS             537..1214
                     /locus_tag="ACG8PN_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4862975.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1165..2346
                     /locus_tag="ACG8PN_002872"
     CDS             1165..2346
                     /locus_tag="ACG8PN_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4862976.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2359..>2671
                     /locus_tag="ACG8PN_002873"
     CDS             2359..>2671
                     /locus_tag="ACG8PN_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4862977.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IE"
BASE COUNT         1002 a          346 c          517 g          806 t
ORIGIN      
        1 ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta
       61 gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca
      121 attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag
      181 aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga atacctgact
      241 atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca
      301 tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa
      361 aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa
      421 gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga cggcaacagt
      481 tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga tattaagtgg
      541 attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt attggggctg
      601 gcggacacgc aaaagtcatt attgaaacct taagattaga aaataagtta atatatggat
      661 tttttgatga taagcaaaat aaaatgcaag atctgaaata tcttgggaaa ataaacgaat
      721 tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct attggtgact
      781 gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag
      841 ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga actgttattt
      901 ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata ataaacacag
      961 gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct ccaggtgtat
     1021 gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt tctcaatgta
     1081 tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact attgttaagg
     1141 atattccctc acattcctta gcttatggca atccagctaa aattaagaaa gaaggaatta
     1201 attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga agaaaaatat
     1261 gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt ggataaattt
     1321 gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt aagttcaggt
     1381 acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga tattgtcttt
     1441 gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg ggctgtacca
     1501 gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact agagagcgca
     1561 ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt gaatttatat
     1621 ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa tgttccagtg
     1681 atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc aggcttattt
     1741 gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc aggtggagga
     1801 gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc aacacaagca
     1861 agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg tctaagcaat
     1921 atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt agctaaacga
     1981 agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat tgaaatgatg
     2041 ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat caatgattat
     2101 aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa cattgaaaca
     2161 agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa aatgtattac
     2221 catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg cttaccttct
     2281 ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa aaaaatattc
     2341 aagtaggtgt gatattttat gaacatagga attattggtt taggttccat ggggaaaaga
     2401 agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt tgatactaat
     2461 gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa tagtctaaca
     2521 gaggcaaaaa atttgtttga aataaattca gtttttgttt gtacatcccc tataacccat
     2581 gaatttatta taaaagaggc tttaaattta gattgtaata tctttacgga aatcaattta
     2641 ttaaatgaat attatgatga aataatcgaa g
//
LOCUS       DAZUKA010000235         2651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000235 DAZUKA010000000
VERSION     DAZUKA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.241"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1188
                     /locus_tag="ACG8PN_002874"
     CDS             join(149..646,649..1188)
                     /locus_tag="ACG8PN_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFS4862978.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            1201..2139
                     /locus_tag="ACG8PN_002875"
                     /pseudo
     CDS             1201..2139
                     /locus_tag="ACG8PN_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            2132..2479
                     /locus_tag="ACG8PN_002876"
     CDS             2132..2479
                     /locus_tag="ACG8PN_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862979.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT          914 a          475 c          589 g          673 t
ORIGIN      
        1 atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca aaatccttaa
       61 atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat aggcgtatac
      121 taaagaaaac acttgaggag tggggaacat ggaaacagaa cgccaaatcc gtgaattgga
      181 aaaagcgatg aatacgacag acaaccgccg gttgtacgag cgtttcctgg cagtcaagtt
      241 ggttcttgaa ggccacaccc ggaaagaagc cgggcacacc atcggccgcg acgagcatac
      301 tgtcagccac tatgtgaaaa actactgcca taatggaatc caaggtttag tctccaaaaa
      361 acaaagcgga cgaccttcac ggctgacaga tgaacaggaa gcggaactgg tccaaatcgt
      421 cgcctaccac actccggagg aagtaggttt caaatcccgt gccaattgga cattggcgat
      481 tgtgtgtgac ctgattctcc gggaatggag ttatggatat acgcaaaaag gcgtggcgga
      541 cctccttcac cggctaggac taagctggac ccgtccgacg tataccctca aaaaagcgga
      601 ttccaagaaa caacaggtat ttttggaaga aaccttcccg agcctaaaaa aaaattgctg
      661 aatagcgata ttgaccacct cctattcgag gacgaaacca tgatccggga ttaccaagcc
      721 attggctgca cctggtttct ccgcggccac cagcgaaaaa tccccactta tggacaacac
      781 aaaggggtaa aattgatagg caccttggat tatgaaaccg gtcggatcat ttgtacagaa
      841 gaaggggcat acgacgcagc ggccttcctc cgttttctgg agcggacgtt gcgggaatac
      901 cccaccggaa aaatcgtgat ggtattggat aatgccctta tccaccacgc caagctgatc
      961 cagtcttttc ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc tccctacagc
     1021 cctcagttga acctaatgga aggcgtttgg aagtggctca aggaatcggt catcaacaat
     1081 gttttttttg accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt agccgatgta
     1141 aacgagcgtc cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag tatttaattc
     1201 aacctatata gttcaggaac aagaagcgag agaagaagag aattagctaa aataatcaaa
     1261 gatgagattc agaaagaaac aggtctaata gcaacagtcg gaataggaga taacccatta
     1321 ttagctaaac ttgcactgga caatgaagcg aagagtagta aagaatttat agctgagtgg
     1381 acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga cttttggggc
     1441 attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat agaagattta
     1501 gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact ccagttatat
     1561 ttccatacca atggaattga tcgtacggat attgctattc caccagaacc aaccaaagaa
     1621 aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga agaagtagaa
     1681 ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca caattgcaaa
     1741 actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc aggtttctcg
     1801 caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta ttgtcttttt
     1861 ttattcaata agttttatga ggggcaggaa gtacgacata taggagtaac ttatagcaga
     1921 ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa aaagattgct
     1981 gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt tacagctatt
     2041 atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac tttaatagga
     2101 ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata gaggacgttt
     2161 aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag acaatgtaca
     2221 acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg cgaaaatact
     2281 gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac gaaacgctga
     2341 aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc ttggtgtatt
     2401 tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttct cggaattatt
     2461 aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat tacgtatgaa
     2521 cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt ggggattgat
     2581 tgcttttgtt tctatattga atccggagca ggataccata agaacagata ctatatattt
     2641 ccatatccac c
//
LOCUS       DAZUKA010000236         2626 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000236 DAZUKA010000000
VERSION     DAZUKA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.242"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1492
                     /locus_tag="ACG8PN_002877"
     CDS             41..1492
                     /locus_tag="ACG8PN_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4862980.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1841..2473
                     /locus_tag="ACG8PN_002878"
     CDS             1841..2473
                     /locus_tag="ACG8PN_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4862981.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          987 a          378 c          516 g          745 t
ORIGIN      
        1 tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact
       61 tgcatcagca gttatattaa tgtcccaact ccttatagca acacctttcc atgtgttggc
      121 aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg aaatgagtaa
      181 ttccgatgta acagcagaga gtagtgaact tttgacaact tcatcaagtg aaagtagtac
      241 gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga cttcaaatgc
      301 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      361 aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag
      421 tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa
      481 ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc
      541 aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa
      601 aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca
      661 gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg
      721 tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa
      781 tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac
      841 ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt
      901 attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag
      961 taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata
     1021 ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt
     1081 taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt attcaataac
     1141 cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca
     1201 gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc
     1261 ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca
     1321 gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt
     1381 aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag
     1441 tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta
     1501 atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg
     1561 agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga
     1621 atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa
     1681 aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta
     1741 gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat
     1801 tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca
     1861 ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga
     1921 ctgtgggaat gaaacgattg tatatcgatc aaacttacta tcaggtagaa ccaaaagttg
     1981 tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg
     2041 taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg
     2101 tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt
     2161 aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt
     2221 acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca
     2281 ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca
     2341 aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga
     2401 gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc
     2461 taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta
     2521 tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc
     2581 tttgattgac aaatatcgag gtaatcatta attattgtgg ataata
//
LOCUS       DAZUKA010000237         2602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000237 DAZUKA010000000
VERSION     DAZUKA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.243"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACG8PN_002879"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACG8PN_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACG8PN_002880"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACG8PN_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACG8PN_002881"
     CDS             2093..2254
                     /locus_tag="ACG8PN_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862982.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          920 a          409 c          446 g          827 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga at
//
LOCUS       DAZUKA010000238         2590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000238 DAZUKA010000000
VERSION     DAZUKA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.244"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8PN_002882"
     CDS             <1..1412
                     /locus_tag="ACG8PN_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4862983.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2590
                     /locus_tag="ACG8PN_002883"
     CDS             1546..>2590
                     /locus_tag="ACG8PN_002883"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4862984.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTAR"
BASE COUNT         1017 a          363 c          520 g          690 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat
//
LOCUS       DAZUKA010000239         2540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000239 DAZUKA010000000
VERSION     DAZUKA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.245"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..245)
                     /locus_tag="ACG8PN_002884"
     CDS             complement(<1..245)
                     /locus_tag="ACG8PN_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862985.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKK"
     gene            407..1516
                     /gene="pdhA"
                     /locus_tag="ACG8PN_002885"
     CDS             407..1516
                     /gene="pdhA"
                     /locus_tag="ACG8PN_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4862986.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1519..2496
                     /locus_tag="ACG8PN_002886"
     CDS             1519..2496
                     /locus_tag="ACG8PN_002886"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4862987.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          846 a          471 c          532 g          691 t
ORIGIN      
        1 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
       61 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
      121 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
      181 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
      241 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
      301 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
      361 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
      421 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
      481 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
      541 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
      601 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
      661 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
      721 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
      781 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
      841 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
      901 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
      961 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
     1021 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
     1081 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
     1141 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
     1201 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
     1261 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
     1321 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
     1381 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
     1441 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
     1501 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
     1561 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
     1621 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
     1681 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
     1741 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
     1801 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
     1861 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
     1921 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
     1981 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
     2041 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
     2101 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
     2161 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
     2221 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
     2281 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
     2341 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
     2401 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
     2461 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
     2521 atggcttatc aatttaaatt
//
LOCUS       DAZUKA010000240         2511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000240 DAZUKA010000000
VERSION     DAZUKA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.246"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..506
                     /locus_tag="ACG8PN_002887"
                     /pseudo
     CDS             186..506
                     /locus_tag="ACG8PN_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            684..2276
                     /locus_tag="ACG8PN_002888"
     CDS             684..2276
                     /locus_tag="ACG8PN_002888"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4862988.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            2269..>2511
                     /locus_tag="ACG8PN_002889"
     CDS             2269..>2511
                     /locus_tag="ACG8PN_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4862989.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          894 a          394 c          510 g          713 t
ORIGIN      
        1 gtggcaggta ataactaatt ttcttatgga attaaaaaaa tacagtaata ctcaaacgaa
       61 gttctcttga agttataaag aatacaataa atataaattt cctttagact atattaaaat
      121 cagcaatcga aggaaaataa aagaagaaaa tggtacgcaa aatctaacta ttagagcaaa
      181 aacaggaagc tgctttggaa gatagtcccg aattgtttcc gatgttaaaa gatcgtttaa
      241 atgaattgac tgaaaaacga agacagttac aaattcgaga gaatgaaatc ttagggatct
      301 ttcaacaaca aggggaaccg attcaagtca aagatgtcca acagatattg actagtttgg
      361 attgctttct tatccagagt gagaagaagc aagttaaagc tttgtatcac acctttattg
      421 agaaaatcac ctttgatcct aatcagaagt agatattgcc gatcccgatg agattcacac
      481 ctactgtgat ttgataattg aatgagtaat atcaaatagc cgtatccaaa ccaaaggata
      541 tggctatttt tgtgttaggg atatacgtca cgtagcttgt tatcaagaat aagtttcttt
      601 atttttttgc tttttctaaa actgagtatg ctaaaataag agggtatata gcaactttaa
      661 ataagagata ggagtgtatc tttttgggtg cagaattaaa tcaaaaatta tttagtgcgg
      721 cagataatct ccgtagcaaa atggatgcgt cagaatataa aaattattta ttgggactga
      781 ttttctataa atatttatcc gaccgtttgt tggaacaagt ggttctttta gcagatgaat
      841 ccttagagga atatgatacg gtaagcaaac aaacgatgtt gtatcgcgaa ttactttcag
      901 atgaagaatc aaaagaagat ctgattgcaa caatcgttga tattttgggg tatgcgattt
      961 cgccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg ttccaattga
     1021 atgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat
     1081 ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga
     1141 cgattaccga agtcattaag aaattgaatg acgtggaagt tttaggtcac gatggggacg
     1201 tgatcgggga tgcttatgaa tttctgatca gtcaatttgc ttcagaagca gggaagaaag
     1261 ccggagaatt ttacacgcct catatggttt ctgatatgat ggcacaaatc gtgacgttgg
     1321 atcaaaaaga acgacgattc tttagtgttt ttgatccaac catgggttca ggttcattga
     1381 tgttaaatgt gcgtaactac ttaactcacc cagataacgt taaatatcat gggcaagaat
     1441 taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggg gtagatgccg
     1501 aagaaatgaa tctccgcaat ggggatacgt tgaataagga ttggccaaca gatgaacctt
     1561 acacatttga tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga
     1621 ccttcttaga cgattctcgg tttaatcgtt atggaaaatt agcgccaaaa tccaaagcag
     1681 attttgcgtt tcttcttcat ggtttctatc atttgaagga aacagggact atggccatcg
     1741 tcttgccaca cggagtttta ttccgtggcg ctgcagaagg ggtcattcgt caaaaattac
     1801 tagaagacgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc ttcgggacat
     1861 cgattccaac gacggtgatt gtattgaaaa agaaccgcca aactcgagac gtattattca
     1921 ttgacgctag tcgagaattt gtcaaaggaa aaaatcagaa taaattatcg gaagaaaaca
     1981 ttcaaaaaat cctcgaaacc tatgctgaaa gaaaagatgt cgagaaatac gctcatttgg
     2041 caaactttga tgaaatcaaa gaaaatgact ataacttaaa tattccacgc tatgtagata
     2101 cgtttgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc aagaagatac
     2161 gacaagaaca gcaagtgcta gaaaaagaac ttttagaagc tatctcttcc ctgcaaacaa
     2221 cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat gagtaatgat
     2281 atacaaccag aaattcgttt cccaggattt acggaagctt gggaacagcg taagtttgaa
     2341 tgcttacttg ataaaaaaga tggagtaagg aggggaccat ttgggagtgc cttaaagaaa
     2401 gaattctttg tatctaatag caattttgtt gtctatgaac aacaaaatgc tatttatgat
     2461 aattacgaaa caagatataa aattactgaa aaaaaatata atgaattaat c
//
LOCUS       DAZUKA010000241         2501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000241 DAZUKA010000000
VERSION     DAZUKA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.247"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..979
                     /locus_tag="ACG8PN_002890"
     CDS             227..979
                     /locus_tag="ACG8PN_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4862990.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1119..2042)
                     /locus_tag="ACG8PN_002891"
     CDS             complement(1119..2042)
                     /locus_tag="ACG8PN_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4862991.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2057..2329)
                     /locus_tag="ACG8PN_002892"
     CDS             complement(2057..2329)
                     /locus_tag="ACG8PN_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862992.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          825 a          487 c          415 g          774 t
ORIGIN      
        1 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
       61 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
      121 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
      181 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
      241 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
      301 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
      361 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
      421 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
      481 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
      541 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
      601 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
      661 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
      721 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
      781 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
      841 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
      901 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
      961 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
     1021 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
     1081 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
     1141 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
     1201 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
     1261 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
     1321 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
     1381 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
     1441 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
     1501 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
     1561 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
     1621 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
     1681 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
     1741 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
     1801 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
     1861 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
     1921 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
     1981 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
     2041 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
     2101 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
     2161 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
     2221 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
     2281 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
     2341 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
     2401 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
     2461 ttataatgat actgtaaata tatctttatt attagtgaat g
//
LOCUS       DAZUKA010000242         2428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000242 DAZUKA010000000
VERSION     DAZUKA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.248"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..813
                     /locus_tag="ACG8PN_002893"
     CDS             79..813
                     /locus_tag="ACG8PN_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4862993.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            852..1994
                     /locus_tag="ACG8PN_002894"
     CDS             852..1994
                     /locus_tag="ACG8PN_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4862994.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          934 a          375 c          431 g          688 t
ORIGIN      
        1 ggggtgtcaa tagttttgta aaatttattt acaacacccc ttttttgagg tatactaatt
       61 ttaggaggtg tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag
      121 atactcggaa caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt
      181 ggtcttatga ttaacaacgg tcaattaatt gacaaaaaga ctatctacga atgggagaag
      241 ggaacctatt taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt
      301 atagaggagt tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga
      361 gatagcattg tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt
      421 caacagttcc caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta
      481 gaaccagaga tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa
      541 attagtcttt tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa
      601 tttgacaata acattctttt cctcactagc agtatagaag aactactaga aagaatggta
      661 gatgaatggc tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa
      721 attactgata atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact
      781 aagaaaaaaa tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag
      841 gagatgattt catggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga
      901 tgtataaagg acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa
      961 gaggttttag tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat
     1021 tatacggtgt taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga
     1081 taactcatca gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa
     1141 ataatcaaat acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt
     1201 gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac
     1261 agactgcaca aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg
     1321 aaaaaacact tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag
     1381 aggagcttaa taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct
     1441 tcttcagatt attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga
     1501 aagacataga cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat
     1561 ttggtaaagt tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag
     1621 atactgagac tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa
     1681 aacttggata caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc
     1741 tatacattcc aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc
     1801 ctcgtattac acctcatggg tttagacata cacatgctag tttattatta gaagcaggag
     1861 aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca
     1921 tctatgcaca tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga
     1981 tggctcatca gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa
     2041 aaaagctaga acccttatat atcaagggtt ctagctccta taacaggttt acccaacact
     2101 gccttccaat gactcttttg ggaatcagag gaaagaatca ggctttatag agatatttca
     2161 tctaacaaat tactttagac tgacagtata tatcagcagg tactgtcatc gggaacgtag
     2221 ccaatatcgt atccaaaaca gcaggtagag aagctatttg aggattcaag ttattctatt
     2281 tcaaccatga agttaagttc attaaaaata aagatagttg gtatttcaat aatttttgta
     2341 tagagttaat cactttattg atgctaattc atcttgattg gtattataat cgtattcaag
     2401 ttgcgtggcg tgcttttgct agaaaata
//
LOCUS       DAZUKA010000243         2389 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000243 DAZUKA010000000
VERSION     DAZUKA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.249"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..647
                     /locus_tag="ACG8PN_002895"
     CDS             12..647
                     /locus_tag="ACG8PN_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS4862995.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            760..1395
                     /locus_tag="ACG8PN_002896"
     CDS             760..1395
                     /locus_tag="ACG8PN_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862996.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(1388..1522)
                     /locus_tag="ACG8PN_002897"
     CDS             complement(1388..1522)
                     /locus_tag="ACG8PN_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4862997.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            1694..>2389
                     /locus_tag="ACG8PN_002898"
     CDS             1694..>2389
                     /locus_tag="ACG8PN_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFS4862998.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNY"
BASE COUNT          929 a          364 c          410 g          686 t
ORIGIN      
        1 aggatctatt aatggacggt atatttggtt ttactggtat tgttttattt attataggtt
       61 taatcatgtt aattgtaaga tttatcaaaa aaacaaataa gaaaacacct atgattctcc
      121 ttattatcgg gattattttt actaccgtag gtttctcgct ttcctcatca aacgaggcaa
      181 atgacaataa caaacaggaa agctcatcat ctgatactca aactactaca tctaacagca
      241 gcaataaaag cctaactcaa tttgaagaat ttttagagac aaataatcat gactggggtg
      301 ctttcttaga tagttattat tctattactc cagctactga gcaaaataca gcttttacta
      361 aatatattag tggtaaaaca tatacttttg aaggaactgt tatcgaatca atgactacta
      421 gaatagccat tatagcagac aaagagtacg atgataaaag ttggaatgat atttctgcaa
      481 cacctaaagt ttcatatgta atttttgcga aggacttaaa caatgctgat acctttacaa
      541 agggcgataa agtaactttt acaggagaaa ttagttcaag aggatcgaac atagaaaaat
      601 cctatgctca atgggatatg attaatagta aagtaagtaa aaaataaatg aaccacgccc
      661 cactaagagt tagattgtgc acaataataa aacctataca aaagagtgcc atcaggagtc
      721 acaatgcttg taaaggaaat gacaaaatag gaggtaacta tggataatag caatgaaaaa
      781 ctaaccaaaa aagaatatct tgaattaata gttgaaggcg gtattggagc aattccttat
      841 attggtggcg ttgtgcagtc actttatttt gggtctaaaa acgagaagcg tttcaaacgc
      901 atcgagaaat tctacaaaaa tttaaacgaa tcattagaaa acattcgcga tcaaattcca
      961 gaagatgttt tcaacttaga gaacaaggac caactcattg gaatattcga agggataaat
     1021 gacgaggtag aaaaatcaaa ggcacaaaat aaaatagata tgtataaaaa tctttataag
     1081 aattgtttac ttcgaattaa caacgcttca tgggataacg aggagtatta tcttcttgta
     1141 ctgaatcaat taacatcgat tgagattcaa ttgttagcat gcttaatgag tcgtggaaac
     1201 gaaaatttca ctggaaatat cagcatgtct ggctattcac aagagctaat agatggtagc
     1261 ttaaatagat tatctgattt tggtctgtta gaaaaaacga ttagctccat agtattaggt
     1321 gatgtcggca aacaaaatat gggctacaaa atatctcatt taggaattca atttattaac
     1381 tcaactctta cttaagtttt ctttctaata tgacatctag acctatcagc aatgcaacaa
     1441 taaaaaacca gacataccaa taatcaacaa ataatctaaa ctctggaagt tcagttattc
     1501 ttttaaaaat cactttaatc ataataaaca cctctgattg gatgatctaa tacacatcca
     1561 attataccat taaaacgaca aatagcacat ccttgcgccg gcaagcaaaa aggatgtgca
     1621 aaaaataata aaacccaaac aatgggcttc tttatagttc ctattgtatc agagaaagag
     1681 agctgattca attatggcaa gattagtcaa acgtggaaat agttggcaat acgaaatttc
     1741 atacaaaaaa gatgacggaa aatacacgaa gataagaaaa tcaggattta agacaaaagg
     1801 agaagcaaaa gatgccgcca acgaattaga atataacttg aacaaaggcc ttaaagggga
     1861 tcgcaaaaat ctattattat cagattactt tgaggattgg atgcaacttt ataaagaagg
     1921 aacagtatct cctatcactt atagaaaata cgaagatacg ttaatgaaca taaagaaata
     1981 tatgccagcg gtattgattt ctgatttaga tagagtggga tatcaacgct ttttaaataa
     2041 gtatgctaaa gaccatgtaa aatctaccgt tattaagttt aataaccata ttagagcgtc
     2101 gttgaaagat gccgtagaag aaggattaat tccgtttgat ccaactagaa aagcagtaat
     2161 caaaggaaaa gattcattga agccaaaaga agataaatat ttagattatg atcaatttaa
     2221 atctttaatg aaactcgtag aagaaaacct ttctgcgcag tactcttctc ctatgctcgt
     2281 gttagttgct ggtgccactg gaatgcgatt tgctgaactt ctaggattaa catgggaaga
     2341 tatcgatttc gaagatcaaa tcatcactat taataaaaca tggaattat
//
LOCUS       DAZUKA010000244         2371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000244 DAZUKA010000000
VERSION     DAZUKA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.250"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACG8PN_002899"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACG8PN_002899"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4862999.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACG8PN_002900"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACG8PN_002900"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4863000.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT          860 a          379 c          498 g          634 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa t
//
LOCUS       DAZUKA010000245         2349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000245 DAZUKA010000000
VERSION     DAZUKA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.251"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..>2349
                     /gene="esp"
                     /locus_tag="ACG8PN_002901"
     CDS             23..>2349
                     /gene="esp"
                     /locus_tag="ACG8PN_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4863001.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          853 a          370 c          481 g          645 t
ORIGIN      
        1 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
       61 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
      121 tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat
      181 ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
      241 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
      301 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
      361 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
      421 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
      481 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
      541 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac
      601 gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga
      661 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
      721 tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc
      781 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
      841 atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac
      901 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
      961 ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
     1021 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
     1081 ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga
     1141 aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa
     1201 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
     1261 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
     1321 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
     1381 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
     1441 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
     1501 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
     1561 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
     1621 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
     1681 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
     1741 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
     1801 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
     1861 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
     1921 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
     1981 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
     2041 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
     2101 aatttcagat gtctataata agtacttcgg taatgcaagt gttgatgcaa acactggtat
     2161 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
     2221 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
     2281 taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt
     2341 caccgcagg
//
LOCUS       DAZUKA010000246         2340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000246 DAZUKA010000000
VERSION     DAZUKA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.252"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..468
                     /gene="dhaM"
                     /locus_tag="ACG8PN_002902"
     CDS             22..468
                     /gene="dhaM"
                     /locus_tag="ACG8PN_002902"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4863002.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            443..1453
                     /gene="dhaK"
                     /locus_tag="ACG8PN_002903"
     CDS             443..1453
                     /gene="dhaK"
                     /locus_tag="ACG8PN_002903"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4863003.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            1481..2086
                     /gene="dhaL"
                     /locus_tag="ACG8PN_002904"
     CDS             1481..2086
                     /gene="dhaL"
                     /locus_tag="ACG8PN_002904"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4863004.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(2182..>2340)
                     /locus_tag="ACG8PN_002905"
     CDS             complement(2182..>2340)
                     /locus_tag="ACG8PN_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4863005.1"
                     /translation="GITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFL
                     INQLIKVR"
BASE COUNT          761 a          393 c          575 g          611 t
ORIGIN      
        1 atatacttga attgttcatt aatgaaagcg aaaccaatcg ttagcaacta tgtaaaagaa
       61 aggaagattc ttgtgaaaaa aagtattttg cttatttctc atagtcaaaa agtaacagat
      121 ggtattaaag aaatgattga gcagatgcaa aaatccgaag aggtgcaaat tttttctctc
      181 ggcggaataa atggcgagat cggatcagac ccgatgaaaa tcgtagaagc agtcaatgaa
      241 tcaagcgatg cagctagtta ttttgttttt gcggatatcg gtagcgcggt attgaactcg
      301 gaactggcaa aagacatgtt ggaagaagag cagcagcaac gttatcatct ggtggatgcg
      361 cctttagtgg aaggagcttt tgcagcagcc atcactgccg gtataacaga tgatactcag
      421 caaatcttaa cagaagcaca aaatgccgga aagaaaggat gggaataaat tgaaaaaaat
      481 catgaatgat ccaagcaata ttgtcgaaga aatgctggaa ggattagtaa aaagctatcc
      541 agaacttgtt catcgtgtag aatcttcccg tgttgtagcc aagaatcaga aagcggaaca
      601 agttggtttg gtttctggtg gaggaagcgg acatgaacca tcccatgccg ggtttgtagg
      661 ggaagggatg ttaagtgcag cggtgcttgg tgatgtgttt acttctccaa ctccagatca
      721 gattcagacg gctattaagg aagccgatag tggaaaagga gttttgctga tcgttaagaa
      781 ttatacaggg gatgcattga acttcgatat ggcaaaggaa ttagctgcta tggacgatat
      841 tgaagtagaa agtgtgatcg tagatgatga tatcgccgtt gaaaatagta cgtatactgc
      901 tggaaaacgc ggagttgcag gtactgtcct tgtacacaaa atcgtgggcg atgcagcaag
      961 aaacggtgct tccctagctg agttgaaaga actgggagaa aaggtcgtac aggcaactaa
     1021 gacgatcggt ctagcgcttc gagcagcaac tgtaccagaa gtagggaaac caggattcga
     1081 actaggagaa gatgagatcg agtatggtgt tggtatccac ggtgagccag gctatcgtcg
     1141 agaaaagatg cagccttcta aagtactggc aaaggaactt gttactaaga ttttagatga
     1201 ttactcgaat ttaccaaaag aagcaggtgt actagtcaac ggaatgggcg ggacgccttt
     1261 gatggaacag ttcgtgttca tgaatgatgt tttagctctg ttggaagaaa gaggcgtcaa
     1321 tgtagttttc cgaaaagtcg gtaattttat gacctcacta gatatgcaag gtctttctct
     1381 aacactgatc gacttgacgg agacacaatg gaaagattct ttagaaagca atgtacagac
     1441 gatttcatgg taaaaaatct caagataaaa gggtgataat atgaaactaa ccgttcaaga
     1501 aatccaagaa tggctcacgc aatttgcaga ggcaatcaat cagaacaaac aatatctcag
     1561 tgatctagat accccgattg gcgatggaga ccatggaaat aacatgggaa gaggggtttc
     1621 agcttatgaa gaagcttttc aagccgatca tcctgaaaca atcagcgata cattcaaagt
     1681 attctctatg gcgatgatat cgaaagtcgg tggggcttct ggtccgttat acggatctgc
     1741 attcatgaac atgatgaaag cgacaaaaga ggtcgattct attgattctc aagaaaaatt
     1801 aggcgaagtg atcgagcagg gaacagcggg tattcaaagc cgaggaaagg cagaagctga
     1861 cgataaaacg atgttagatg tttggctgcc agtcacagag gcattgaagg cagggaattt
     1921 aacaaaagaa atcattgacc aggcaaaaga acacaccaaa gtcttggtag ctaaaaaagg
     1981 acgagcttcc tatctaggag agcgtgcaat cggacatatc gacccaggag ctgcttctag
     2041 tgccatttta ttttcaacgc ttttagatgt gatcaatcaa ccttaagttc aataaaactg
     2101 gctctttgtc aataaggact gatgggttgt acaaaatcaa atctgggtca gaatgaacct
     2161 cattctggct cagatttttt gttatcttac tttgattaat tgattgatca aaaagattct
     2221 aattctcatg ttctcaaatg atttaaatcc gtaggatact cgtttcattg tctttatatg
     2281 ggtattcttc gcttctattt ttccattggt gtaaggatag atcatcgcgt tggtgatgcc
//
LOCUS       DAZUKA010000247         2330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000247 DAZUKA010000000
VERSION     DAZUKA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.253"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..165)
                     /locus_tag="ACG8PN_002906"
     tRNA            complement(94..165)
                     /locus_tag="ACG8PN_002906"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Glu,seq:ttc)
     gene            complement(177..250)
                     /locus_tag="ACG8PN_002907"
     tRNA            complement(177..250)
                     /locus_tag="ACG8PN_002907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ile,seq:gat)
     gene            complement(263..333)
                     /locus_tag="ACG8PN_002908"
     tRNA            complement(263..333)
                     /locus_tag="ACG8PN_002908"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(338..410)
                     /locus_tag="ACG8PN_002909"
     tRNA            complement(338..410)
                     /locus_tag="ACG8PN_002909"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Phe,seq:gaa)
     gene            complement(414..486)
                     /locus_tag="ACG8PN_002910"
     tRNA            complement(414..486)
                     /locus_tag="ACG8PN_002910"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asp,seq:gtc)
     gene            complement(505..578)
                     /locus_tag="ACG8PN_002911"
     tRNA            complement(505..578)
                     /locus_tag="ACG8PN_002911"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Met,seq:cat)
     gene            complement(591..680)
                     /locus_tag="ACG8PN_002912"
     tRNA            complement(591..680)
                     /locus_tag="ACG8PN_002912"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(642..644),aa:Ser,seq:tga)
     gene            complement(703..774)
                     /locus_tag="ACG8PN_002913"
     tRNA            complement(703..774)
                     /locus_tag="ACG8PN_002913"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(739..741),aa:Glu,seq:ttc)
     gene            complement(796..883)
                     /locus_tag="ACG8PN_002914"
     tRNA            complement(796..883)
                     /locus_tag="ACG8PN_002914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Ser,seq:gct)
     gene            complement(908..981)
                     /locus_tag="ACG8PN_002915"
     tRNA            complement(908..981)
                     /locus_tag="ACG8PN_002915"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Ile,seq:gat)
     gene            complement(995..1065)
                     /locus_tag="ACG8PN_002916"
     tRNA            complement(995..1065)
                     /locus_tag="ACG8PN_002916"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Gly,seq:tcc)
     gene            complement(1067..1142)
                     /locus_tag="ACG8PN_002917"
     tRNA            complement(1067..1142)
                     /locus_tag="ACG8PN_002917"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Asp,seq:gtc)
     gene            complement(1161..1234)
                     /locus_tag="ACG8PN_002918"
     tRNA            complement(1161..1234)
                     /locus_tag="ACG8PN_002918"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Met,seq:cat)
     gene            complement(1247..1336)
                     /locus_tag="ACG8PN_002919"
     tRNA            complement(1247..1336)
                     /locus_tag="ACG8PN_002919"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1298..1300),aa:Ser,seq:tga)
     gene            complement(1360..1433)
                     /locus_tag="ACG8PN_002920"
     tRNA            complement(1360..1433)
                     /locus_tag="ACG8PN_002920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1397..1399),aa:Ile,seq:cat)
     gene            complement(1447..1520)
                     /locus_tag="ACG8PN_002921"
     tRNA            complement(1447..1520)
                     /locus_tag="ACG8PN_002921"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1484..1486),aa:Met,seq:cat)
     gene            complement(1548..1620)
                     /locus_tag="ACG8PN_002922"
     tRNA            complement(1548..1620)
                     /locus_tag="ACG8PN_002922"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1585..1587),aa:Ala,seq:tgc)
     gene            complement(1640..1713)
                     /locus_tag="ACG8PN_002923"
     tRNA            complement(1640..1713)
                     /locus_tag="ACG8PN_002923"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1677..1679),aa:Pro,seq:tgg)
     gene            complement(1723..1796)
                     /locus_tag="ACG8PN_002924"
     tRNA            complement(1723..1796)
                     /locus_tag="ACG8PN_002924"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1760..1762),aa:Arg,seq:acg)
     gene            complement(1807..1892)
                     /locus_tag="ACG8PN_002925"
     tRNA            complement(1807..1892)
                     /locus_tag="ACG8PN_002925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1856..1858),aa:Leu,seq:taa)
     gene            complement(1909..1980)
                     /locus_tag="ACG8PN_002926"
     tRNA            complement(1909..1980)
                     /locus_tag="ACG8PN_002926"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1946..1948),aa:Gly,seq:gcc)
     gene            complement(1992..2064)
                     /locus_tag="ACG8PN_002927"
     tRNA            complement(1992..2064)
                     /locus_tag="ACG8PN_002927"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2029..2031),aa:Thr,seq:tgt)
     gene            complement(2082..2163)
                     /locus_tag="ACG8PN_002928"
     tRNA            complement(2082..2163)
                     /locus_tag="ACG8PN_002928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:Leu,seq:tag)
     gene            complement(2171..2243)
                     /locus_tag="ACG8PN_002929"
     tRNA            complement(2171..2243)
                     /locus_tag="ACG8PN_002929"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2208..2210),aa:Lys,seq:ttt)
     gene            complement(2247..2319)
                     /locus_tag="ACG8PN_002930"
     tRNA            complement(2247..2319)
                     /locus_tag="ACG8PN_002930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2284..2286),aa:Val,seq:tac)
BASE COUNT          636 a          642 c          543 g          509 t
ORIGIN      
        1 cttagcacaa tcatccataa agataactct gctaagcttt ttaaaatcat tctttcagat
       61 aaaaagaaaa agatactcaa aaagtatctt ccatgacccg tacgggattc gaacccgtgt
      121 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaataa aataaatggg
      181 cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag
      241 ctataggccc ataaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg
      301 aaggctgtgg ttttaccact aaactacacc cgcataatgg cgcgggacag aatcgaactg
      361 ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc aaacggttcc
      421 gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac
      481 ggaaccaatg tttttagttt ttaattgcgg gagcaggatt tgaacctacg accttcgggt
      541 tatgagcccg acgagctacc agactgctcc atcccgcgat aatatgaaaa aggaggataa
      601 gggattcgaa cccttgcacg gttttacccg cctgacggtt ttcaagaccg ttcccttcag
      661 ccggacttgg gtaatcctcc atgtaaaact atattaaatt aatgacccgt acgggattcg
      721 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
      781 aacaaaatat tgaaacggag aaggagggat ttgaaccctc gcgccggttt cccgacctac
      841 acccttagca ggggcgcctc ttcagccact tgagtacttc cccaaaacca ataagatttt
      901 gtactaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg cgtgcgctct
      961 aaccagctga gctataggcc cataaaaaac taaaagcggg tgacgagaat cgaactcgcg
     1021 acaacagctt ggaaggctgt ggttttacca ctaaactaca cccgcatggc ggttccgacg
     1081 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
     1141 ccagtgtttt tagtttttaa ttgcgggagc aggatttgaa cctacgacct tcgggttatg
     1201 agcccgacga gctaccagac tgctccatcc cgcgataata tgaaaaagga ggataaggga
     1261 ttcgaaccct tgcacggttt tacccgcctg acggttttca agaccgttcc cttcagccgg
     1321 acttgggtaa tcctccatat gaaacttaca acgccacaat ggaccttgta ggactcgaac
     1381 ctacgaccgg acggttatga gccgtctgct ctaaccaact gagctaaagg tccaggctct
     1441 taaaaatcgc ggcggagggg atcgaacccc cgacctcccg ggtatgaacc ggacgctcta
     1501 gccagctgag ctacaccgcg aaaaagatat tcattattta aatgaaatgg agcctagcgg
     1561 gatcgaaccg ctgacctcct gcgtgcaaag caggcgctct cccagctgag ctaaggcccc
     1621 gtgtttaaat ttataattat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa
     1681 accaagtgct ctaccaagct gagctacttc ccgtttaatt aacgcgccca agaggagtcg
     1741 aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat gggcgcatat
     1801 ttgaaatgcc gaggaccgga atcgaaccgg tacggtgatc actcaccgca ggattttaag
     1861 tcctgtgcgt ctgccagttc cgccaccccg gcgcgattgc tttggcaaag cggaaaacgg
     1921 ggttcgaacc cgcgaccccc accttggcaa ggtggtgctc taccactgag ctatttccgc
     1981 gtgtttaaag atgccggcta aaggacttga accctcgacc ctctgattac aaatcagatg
     2041 ctctaccaac tgagctaagc cggcatgtaa aacttaaatt atgcgggtga agggacttga
     2101 acccccacgc cgtaaggcgc tagatcctaa atctagtgcg tctgccaatt ccgccacacc
     2161 cgcaaaaata tgagccgtac agggctcgaa cctgtgaccc tctgattaaa agtcagatgc
     2221 tctaccaact gagctaacgg ctcatatgga ggttaacggg atcgaaccgc tgaccctctg
     2281 cttgtaaggc agatgctctc ccagctgagc taaacctcca tggataactt
//
LOCUS       DAZUKA010000248         2281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000248 DAZUKA010000000
VERSION     DAZUKA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.254"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..2280)
                     /locus_tag="ACG8PN_002931"
     CDS             complement(151..2280)
                     /locus_tag="ACG8PN_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4863006.1"
                     /translation="MLNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVA
                     LIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTR
                     GTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQL
                     GNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVV
                     AERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDR
                     VGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKV
                     EEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTI
                     TSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAV
                     KASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGID
                     LPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANG
                     GKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTD
                     PYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPN
                     LLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          584 a          507 c          413 g          777 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 t
//
LOCUS       DAZUKA010000249         2270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000249 DAZUKA010000000
VERSION     DAZUKA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.255"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG8PN_002932"
     CDS             191..445
                     /locus_tag="ACG8PN_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4863007.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG8PN_002933"
     CDS             446..763
                     /locus_tag="ACG8PN_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4863008.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACG8PN_002934"
     CDS             1322..2257
                     /locus_tag="ACG8PN_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863009.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DAZUKA010000250         2249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000250 DAZUKA010000000
VERSION     DAZUKA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.256"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1919
                     /locus_tag="ACG8PN_002935"
     CDS             57..1919
                     /locus_tag="ACG8PN_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4863010.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1919..>2249
                     /locus_tag="ACG8PN_002936"
     CDS             1919..>2249
                     /locus_tag="ACG8PN_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4863011.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          806 a          336 c          445 g          662 t
ORIGIN      
        1 tcatattgtc gttagtttag aaaacgctta tattagcaat gtaaggagag atgataatgc
       61 agcagctaac aaaaagagaa aggcagcttt tgatttactt ggtggaaatg tctgaaccgg
      121 taacaatcaa aaagttggct gaaaaagaaa atgttagtgt tcggacgata aaatacgatc
      181 tagatgatat aagagagtgg ctgaatgagc ggaagcaagt cctatatagc aaacgtagtc
      241 agggaatttg gatgaatatt agtagctctg atcgaatcaa attaaaaagc gaattgatgg
      301 atgttaatcg ttttgaaata tttgcagatc aaagtctccg aatcaaacgt ttactaatta
      361 tgcttttagt aacaaaagaa gctattactg gaatgaaaat ggccaacgca ttagatgtca
      421 gcaaagatac gatcatgaat gatttagatg tgctggaaaa taattttctt aaggaaggat
      481 tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat aggagaagaa cgtctagttc
      541 gattaacgat tgaagagatt cttcaaaaag agttcacgga ttatgacatt tataaattaa
      601 tgtcattgtt attgaatggc ggtgagactg aatactttga aatgtattct gctactgcta
      661 cacctatcca agaggtattt aatcaagtaa tcattcgaat gcgacactta ctggaatttg
      721 aaaatctaga gaaactgaat tacgcggaat tactaaatat tctgattcga gtgacaattg
      781 ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta tcaattggtt gctgaacaag
      841 aattagacaa aaacgatctt tcttatcgcg tcatgcagca ggtctttgat tattatcagt
      901 tgccactgtt cgaggatgaa tatcattata tctatagtga tacattcgac acgattcacc
      961 agcaagacgt catgaaacta gttgagaatg tgatttataa agtaagtaaa gcaatcaacc
     1021 attcttttta tcaagatcca caattgttga cgaatctgta tgctcattta tccatgcgat
     1081 tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt taaagaagat atcaagcaga
     1141 aatacccgga attatttgat gcgatcaagc aagcagttcg acaagaaata agtgaacaaa
     1201 atattctgat caatgattct ttcgtggcat atattgcctt acactttttg gtttcctacg
     1261 aaagagaaga agatctacga agcgttagag ccgtctacat ttgttcaaca gggttgggtg
     1321 tgactagtct gatcaagcaa aagatcaatg aagaattaaa taatatcact atcgtggcat
     1381 ttgcttcagt attgaatgcg caagaaatga ttgctaccaa agatcctgat ttggtgatta
     1441 gtatttttcc aattaatggc atcgatcgtc ctttcatcaa agtccatcca ttacccactg
     1501 aacaggactt gaaaaaaatt caacaaataa taaaaaaaat acttgcttct tccaatcagc
     1561 aggaaaggca taacttgaat cttagagaaa cgtccatcaa taaagatact ttagaagact
     1621 ttagcaagga attgattttg aaagcttata cgatttatga acgtttactt gtcattttgg
     1681 aagcagaatt gatccaagaa tatcgagaag cttttttgct tcatgtattc ttaatggttc
     1741 atcgtatcac atttgatcaa caatatatgg cagaaggtcg tcaagtagac cagattattt
     1801 tagacaaagg aacactggta ggaaaaatcg aagcagtttt tgcagaaaat aatttaggaa
     1861 tcaatcagtc agaaatcagt gcattattct catatataaa agaaggagcg tcaagttagt
     1921 gaaaatgtca gacgaggctt taaaaattat cgaggaaagt caaaatcggg aaaagttaaa
     1981 acaagtgatt gatctaattg aagagcgatt agtagaacat gctattgtac caaccgagtt
     2041 gcagtggacc attctaatta atcacttgaa tgagatgctg aaacgttctc aaagaaagga
     2101 aacgattccc gtagtcgatc gggatttgtt caaagaagta tccaaagaag ccataactat
     2161 ttctgatgaa atagttagaa gtatcggtaa tttaaccgat gatgaaatat atgttttgtc
     2221 gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DAZUKA010000251         2223 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000251 DAZUKA010000000
VERSION     DAZUKA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.257"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..>2223
                     /gene="acm"
                     /locus_tag="ACG8PN_002937"
     CDS             108..>2223
                     /gene="acm"
                     /locus_tag="ACG8PN_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4863012.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          872 a          343 c          441 g          567 t
ORIGIN      
        1 tttttagtta aaagattgga aatgagtggg ggaggaattg aagaaaggat ttttgagaga
       61 tgatatagta ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta
      121 gaaatattat tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc
      181 atgtctatgc ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc
      241 caacgaatat tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac
      301 aaaatatcca accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg
      361 gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa
      421 cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag
      481 tagaaggttg ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa
      541 atacgggacc attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc
      601 cagcttctgg ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag
      661 atactaagca cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac
      721 cagttaaaat cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa
      781 ttaatcaaat acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat
      841 ttttacaaga tttccccagc gctacattta atttttcagt aactgataat tacattgaaa
      901 taactatacc aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca
      961 tagaaaaccc agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta
     1021 ataaacctgc tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg
     1081 gtggagtcaa cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag
     1141 agattggtat cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag
     1201 gacagtctag tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc
     1261 aagtaggaga ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac
     1321 taagttcaaa tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac
     1381 ctatttataa caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa
     1441 ccactccaag accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac
     1501 cagttcctga tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag
     1561 atatccaaca gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc
     1621 aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta
     1681 caaatgagaa taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga
     1741 accaagacgg caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga
     1801 tccaacacaa agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa
     1861 aatacagaga cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca
     1921 gtacgacgat tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat
     1981 ctataccagg tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga
     2041 aaattagaaa tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg
     2101 aacaaagaag caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa
     2161 aaagcattct tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat
     2221 tga
//
LOCUS       DAZUKA010000252         2189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000252 DAZUKA010000000
VERSION     DAZUKA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.258"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8PN_002938"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8PN_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1952)
                     /locus_tag="ACG8PN_002939"
     CDS             complement(987..1952)
                     /locus_tag="ACG8PN_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4863013.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZUKA010000253         2163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000253 DAZUKA010000000
VERSION     DAZUKA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.259"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..1015
                     /gene="msrA"
                     /locus_tag="ACG8PN_002940"
     CDS             182..1015
                     /gene="msrA"
                     /locus_tag="ACG8PN_002940"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4863014.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1095..2084
                     /locus_tag="ACG8PN_002941"
     CDS             1095..2084
                     /locus_tag="ACG8PN_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4863015.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          789 a          358 c          432 g          584 t
ORIGIN      
        1 aaggtaaatt aacttttcat ttataaaata aattgattaa aaaggagaat ctgtcgataa
       61 ggattctctc ttttttatac gaaaaatctg tagggggatg aagtaatcgc ttatttcctc
      121 actttatgat aaattacagc taaagtcact agcttgtctt cttcggaaag gaggagaatc
      181 tatggatgag gaacaaatat tcattcaaac actttataat ctgattttga atccgaatac
      241 acgagattgg gaacgaaaag tattgattca gacaaagaat gacataagag aaaatatatc
      301 cgttaaagaa cagttatcaa aactagaagc aacactacgt ccattagcta tccgaatgaa
      361 tctgacgcct gatgtaatgg acttttattt attgattacg gaaggatttg ataaggagca
      421 aaaatatgac ttttccaagc atgccatgca agatgctgac tatcaagaac gtgcagtatt
      481 tgcaggggga tgtttctggt gtatggtcga gccgtttgaa tcgaaaaaag ggatcctatc
      541 tgttttgtca ggatacacgg gcggacatgt tgaaaaacca aattatgatc aagtaagtgg
      601 tggttacaca ggtcatgtgg aagctgtgga gatcatttat gacacaagag aaataagtta
      661 tagcgagttg ctgacaatct attggcaaat tactgatccc acagatacat ttggacaatt
      721 tcaagataga gggaagcaat atcgtccagt gattttttat caagtcgaac gacaaaaaga
      781 attagcggaa caatcaaaac agaaactaga cagttcagga actttccacc aaccaatcgt
      841 cacaaaaatc gaaccagccg gtacgttttg gccagcagaa aactatcatc agcaatttta
      901 caaaaaacaa ccgaaacgtt ataaaaaaat ccagcaagca cgtaatcaat ttctgatcta
      961 tcaacgagta aaaaacaaat ggcaaaaaaa tatacgaaaa aatcattttg attaactcct
     1021 tttctttaaa atgtagtaaa ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat
     1081 gaggaggaga aaaattgtat attgcagata gtgcacgtta tgaaaaaatg gaatatcgta
     1141 aatccggtca ttcaggtttg aaattaccga ttttatctct cggattatgg caaaattttg
     1201 gtgattatga tcctatccat aatcaaagag agattttaag aggcgcattc gacatgggaa
     1261 ttacccattt cgaccttgcg aataattatg gaggccctgc tggagctgca gagaaaaact
     1321 ttgggcgtat ttttagagaa gattttcaag cctaccgtga tgaactgatt atttctagca
     1381 aagcaggtta tcatatgtgg gaagggccat atggggaatg gggctctcgt aaatccatca
     1441 tttcaagttg tgaccaaagt ctccaaagaa tggggctaga ttatgttgac atcttctatc
     1501 atcatcgccc agaccctgat acacctttgg aagaaacagc agaagctttg atgcaacttg
     1561 taagacaagg aaaagcatta tatattggta tatccaacta taatggagaa gatacaaaaa
     1621 aaatgactga aattttaaaa agaaaggaag caccgttcat tattcatcag atgcgctaca
     1681 atatgttttc tcgtgcacta ctagaagacg atctatctcc agtattagaa gaagaagggc
     1741 taggggcaat cacgttcagt ccgcttgctc aagggttgct gacaaatcgt tatctacatg
     1801 gtattcctga agattctaga gcacatcgta aggaaatacc attcctttca gaagaacaag
     1861 ttggatcaac tctggaaaaa atcaaagcac tgcaaacaat cgccgtatca cgaggacaat
     1921 cattagcgca aatggccctt gcatggaatt tgcgacaaaa atctgttaca agtgtactgg
     1981 tcggagcaag tcggttaagt cagttacaag aaagtgttcg aatgatggat aatttagatt
     2041 tctcaccaga agaagaattg agaatcgacc aaattctaga atagaaacga tgtaatcaaa
     2101 ctttttatcc atatattaaa aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc
     2161 tgt
//
LOCUS       DAZUKA010000254         2139 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000254 DAZUKA010000000
VERSION     DAZUKA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.260"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..1028)
                     /gene="cysK"
                     /locus_tag="ACG8PN_002942"
     CDS             complement(102..1028)
                     /gene="cysK"
                     /locus_tag="ACG8PN_002942"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4863016.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1168..2055)
                     /locus_tag="ACG8PN_002943"
     CDS             complement(1168..2055)
                     /locus_tag="ACG8PN_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4863017.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          591 a          478 c          357 g          713 t
ORIGIN      
        1 acgtctctat tcaaataatt tttctcatca taaatttttg tgtgttcttt tcatctttaa
       61 aagtttttta cataaaccta tctggagaaa aaccaagtgc cttatatctc ttggtaaagg
      121 gctgtagata aataacgttc accattgtcg ggaaccacag ctaatacctt cttgcctttt
      181 cctaattctt ttgcgacttt tagcgcggca gcaattgcag cgccagatga gatgcctact
      241 aaaatccctt cttttcgccc aacttctcta gctgtttcca tcgcttcgtc tccactaata
      301 gaaaggactt tatcatacac agaagtatcc agtgttttag gaacaaaacc tgtaccgatt
      361 ccttggattt tgtgaggtcc cgcttctttc ccttctaaaa tagcagattc tgctggttct
      421 accccgatta attcgatttt cggatacaca cgttttaatt ctcggccagc gccagtgatc
      481 gttcctcctg taccgatacc tgcaacaaac gcgtccaagc catttactcc aaatgcttga
      541 tgtatctcag gaccagtcgt cttctcatgc acaagaggat ttgcttcatt ttcaaattgt
      601 aatggtaaaa agtagccgtt ttcttttgcc aaacgttctg cttctctgat tgatccagaa
      661 ataccatccg caccaggagt caaaatcaac tctgcgccgt accctttcat caataaacgt
      721 ctttcgatac tcatcgtctc aggcatcacg atgattactt tatatccttt tgcaactcct
      781 accatcgaca atccgattcc tgtatttcca gaagtaggtt caatgatagt atcacctggt
      841 ttcagtagac catcgtgttc tgctttttct atcatactta aagcaatacg atcttttaca
      901 cttcctcctg gattaaaaaa ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg
      961 attttattta atttgacgat tggcgttttg ccaatcaatt cggtgactga gttatagata
     1021 gctgtcatct tcaattcctc cttgtctttg ctcttcttac ttatcataat acaatctgtt
     1081 catttatggc gagtgattcg tctttgacta gcaaaaaaaa gacaaagata taccatatat
     1141 ggtaatttct ttgccttttt atctaaatca aacgtttagt tcttgaccaa ctaataaaga
     1201 ataactggac atagacatgc catttttcgc cattagatca tcaacagaca tgccaaattt
     1261 ttgtgcaata tcccatagcg tatcacctga cacgactgta tatgtctgac cattatttgt
     1321 atttacatct gttgttgaag atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt
     1381 acttgtggtt gttgtagccg aacctgttga tggcgtatca aaacgggtta aattataagt
     1441 attaatcaat gagttcaatt tggccccata atttggatcg gtagcataac gacctgtcaa
     1501 gtacgctgtt gcatcataaa aacttgttgt attgcttttc cacacgccag cataatgata
     1561 atcgcccgtg gaaaaactgg ttgaacgaag aacattcgca tggtcttgga aggattccca
     1621 ataagaagaa tattttcgga atggctcatt catcgttacc cattgtccgt ttacatattc
     1681 tttggttgcc atataaacga cctgtccgtt atagcttcct ttcacaccga atagattata
     1741 atttggcgca ctagctaatc cggaagtgcc atatgcactt tccaacaacg cttgagcgat
     1801 catgactgaa gcatacaagt cattacttgc agcaacttct gacgcagacc agccgatttg
     1861 atcgataaac gcttgaggat cagcgactgt ttgagatcct tgttcatctg catgaacatg
     1921 ttgcggtacc gctgtaacca tcatcggccc aactaaaaga ctcgttccaa ttaaaggagc
     1981 aatcgctttt ttattgatca ccatttttct tttaggtcta tggtgccggg cactgcgggt
     2041 cttcagctct tgcattcaaa ttcctcctca aaataaaaaa acttaataaa aagttctctt
     2101 cttaaaacaa acttaattaa ttaaattata cttttaatt
//
LOCUS       DAZUKA010000255         2106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000255 DAZUKA010000000
VERSION     DAZUKA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.261"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..164
                     /locus_tag="ACG8PN_002944"
     CDS             <1..164
                     /locus_tag="ACG8PN_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4863018.1"
                     /translation="ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHAN
                     EVLKIAEEK"
     gene            165..917
                     /locus_tag="ACG8PN_002945"
     CDS             165..917
                     /locus_tag="ACG8PN_002945"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4863019.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            922..1869
                     /locus_tag="ACG8PN_002946"
     CDS             922..1869
                     /locus_tag="ACG8PN_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4863020.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          735 a          279 c          421 g          671 t
ORIGIN      
        1 aaataagttt tgaagaaaac attgttaaag aaatgcctga atctgctaat gatatgtatg
       61 agaaggagtt gaaatatttc tttgaaaacg tagtaacagg caaagaaaac tggaatgatt
      121 tatatcatgc aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa attttattta
      181 ctatttgtgg tagagctggg tctaaggggt ttaaaaataa aaatttgaaa tatttggcag
      241 gaaagcccct agtccatttc gctttagcaa tgatagatat ctatcaacaa aaacatcctg
      301 aacataattg cacgacttgt ttgaatacgg acagtaatga gttagtagaa ttgacgacta
      361 agttaaatcc gtctgtgaaa attgtagaac gtaaacctga attagctaca gatactgtag
      421 ggaaaattga agtgatacgt gattcattct tacaactaga aagagaagat aatatatttg
      481 atttaattat agatttagat ttgacttcac ctttacgtga agtttctaat cttgaagaat
      541 taatagatga acatctaaaa aatttagata aagacgtcta cttttcagtt gtttctgcaa
      601 gacgtaatcc ctattttaat atggtaaaaa gaattgaaaa tgaagttact atagttaata
      661 aatcaagata cacatctagg caacagacac ctgaagtttt tgaaatgaat gcctcaatgt
      721 attcttttaa acctgaattt ctaaaaaaaa gtaattatat ttttgatggg caatgtggga
      781 taataaaaat gagagattat ctaattttag atattgatag tgaagaagat tttaaatggt
      841 tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat atctataatt
      901 atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc tggcttcatt
      961 ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt cgatgactta
     1021 tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat tgagggagat
     1081 gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga ttatatcttt
     1141 catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga aacacatcgt
     1201 gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca acctgattta
     1261 aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac tttaccaaaa
     1321 aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt cgctgcagaa
     1381 caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt tcgttttttc
     1441 aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat ttctattctc
     1501 gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt atttggtgat
     1561 ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt acttttagtt
     1621 gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca
     1681 cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac acttgaatac
     1741 aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa gatccagtca
     1801 attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt gaaaactgaa
     1861 atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt gtcaataagg
     1921 actgatgagt tttgtaaaat caaatctggg atagaagggc tctttgtcaa atgatgttgg
     1981 taaagagaaa tcttagaata atttggacag aacagaaaga agaaaacctc tttctgttct
     2041 gtttatttgg ttaaaaatgt tttgccatga cgaatcataa ttcgtagttt gaaattgtaa
     2101 aagttg
//
LOCUS       DAZUKA010000256         2106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000256 DAZUKA010000000
VERSION     DAZUKA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.262"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..455
                     /locus_tag="ACG8PN_002947"
     CDS             <1..455
                     /locus_tag="ACG8PN_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863021.1"
                     /translation="FQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEE
                     LKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYT
                     DVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(516..881)
                     /locus_tag="ACG8PN_002948"
     CDS             complement(516..881)
                     /locus_tag="ACG8PN_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4863022.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(957..1202)
                     /locus_tag="ACG8PN_002949"
     CDS             complement(957..1202)
                     /locus_tag="ACG8PN_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4863023.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1272..1526)
                     /locus_tag="ACG8PN_002950"
     CDS             complement(1272..1526)
                     /locus_tag="ACG8PN_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863024.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1690..2013)
                     /locus_tag="ACG8PN_002951"
     CDS             complement(1690..2013)
                     /locus_tag="ACG8PN_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863025.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          796 a          359 c          370 g          581 t
ORIGIN      
        1 attttcaggg ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag
       61 caatggcagg ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag
      121 ccacgacaga ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac
      181 gatgcatggg aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt
      241 gtagaagaag caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat
      301 cgtatacaga cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt
      361 ctagggaagt tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg
      421 aagataaccc taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat
      481 gaagtaagag gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc
      541 tccaaatttg ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg
      601 ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga
      661 tttttccact ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga
      721 tgatattgga ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc
      781 tttccccagc catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga
      841 cgaggaagct ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat
      901 atagattgtt aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac
      961 ttcagtttgc ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag
     1021 tcagcgatat agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga
     1081 tatttgaata aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca
     1141 gttcttaaga aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc
     1201 atttaatcac ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca
     1261 atcaatagta ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa
     1321 gacaaaacca gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa
     1381 ttaaaaactt tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt
     1441 tctttgttcc aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa
     1501 aacaaaactc tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag
     1561 ataacatgtt attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc
     1621 aatgaaaaat gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat
     1681 gtgtcttatc tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa
     1741 aaagaggacg atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac
     1801 atatttaaca aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat
     1861 catgacaaga agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc
     1921 tgaggtttga taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat
     1981 cgcaataaat ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt
     2041 ttctaaaata agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa
     2101 taagaa
//
LOCUS       DAZUKA010000257         2062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000257 DAZUKA010000000
VERSION     DAZUKA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.263"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1368
                     /locus_tag="ACG8PN_002952"
     CDS             202..1368
                     /locus_tag="ACG8PN_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS4863026.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT          730 a          281 c          404 g          647 t
ORIGIN      
        1 ggtagttcat gtaaagtgat ttcaactata gaggagcatt atgagaaaaa taaaaatgaa
       61 tttattaaat atcgaacctc gatgattttg aagcaaaaaa attttttcta ttaaataata
      121 ttgattgaat aaggaatatg ttcaaaagga taacagctaa aatactataa aatgaaattt
      181 gattggagcg tatggaagaa tatgaaaatt tcagttttcg gattaggcta tgttggtcta
      241 gcaaattcac tattattagc acaaaaagag caggtcaaag cttatgatat cgtggaagaa
      301 aaaatcacga tgttacaaaa tggcatctca ccaatagaag ataaagaagt tcatgaattt
      361 ttaaaacggg atgacttaaa tgtcgaattt acgtcaaatt ttactgatgc agtaaacttc
      421 ggtgattact tgattattgc aactccaaca gattatgacg aaaagaaaaa ctattttaat
      481 acatcaacag tagaagatgt aattgagaag gctttagcaa ttcgtccaga ttcaacattc
      541 attattaagt cgacagttcc agttggctat gcaaatgaaa ttaaagcgaa atttggtaca
      601 gaaaacatca ttttttcacc tgaatttttg cgtgaaggaa aagctttgta cgacaactta
      661 tatccatcaa gaattatagt tggagaatgt tctgctcgtg gtcgcgagat tgcaactatg
      721 tttcaaagaa acgcatttaa caaagatgca tcagttcttt tgactcactc aactgaagca
      781 gaagctatta aattattctc gaatacatat ttggcattgc gagtggcgta tttcaatgaa
      841 ttagatacgt atgcagaatt tcgtgggctt gatagtaagc aaattattga aggtgtcggt
      901 ttggatccac ggatcggttc gcattataat aatccttctt ttggctatgg tggatactgt
      961 ttacccaaag acacaaaaca attaagagcc aactatgaaa aagtaccaag caatatcatc
     1021 agtgcaattg ttgatgcaaa tacaacaagg aaagatttta ttgctggaca gattttggcg
     1081 aaaaaaccaa atgttgttgg aatctatcgt ttaacgatga aggccggttc agataatttt
     1141 cgatattcgt cgatccaagg aattatgaaa cggattaaag ccaaaggaat tgaagtggtt
     1201 gtttacgagc ctacattgga tgaagatgaa ttttatcatt cacaagtatt gaaagatatt
     1261 aatgaattta aaaatatttc tgatgtgatc gtatctaatc ggtttgaaga tattttaaag
     1321 gatgttgaag aaaaagttta taccagagat atctttggtg ataactaaat aataatgaac
     1381 gggtacatgg aggaaaatat tttagatggt gactcgaaat aaatgtttgt tctcattgca
     1441 tctgtatcta taccgataag tttaaaaatt ccgattagga aaatgaagtt ggctatcctt
     1501 gcgaggaatt tattgctgga ttattttatg aactaaagca agacatcatc agaaaaaaat
     1561 ccaaaatttg acaatgggat tgatcgttta ccagctgtaa gcgaagtatc tgacacgaaa
     1621 atggaaaatg caattactgt tattaccaat agcttttttt catcgatcaa gtatttgaaa
     1681 acattatttg tttgccaagc tgatcaatgt tctttcagac ttggaagaac attgatcagc
     1741 tcacaagtaa tgattgaagt ttaggttatg ggataaagga taagtgaaat aaaagtcgtg
     1801 tctgcatctt tgttagtacc agtaaatttt tctactacgg cgctagtcac gacagtccag
     1861 gcatctgaaa ttgaagatgc aaatagagtt tctgctagtt taggtattga tgttactgta
     1921 ttagcattaa tcttagagcc agtagcatcc aataggtgta gctatagttc taatagtata
     1981 aaacaatgca gaggatatga ttgctttatg tctcggtgtg tcaggtcaaa atagagttcc
     2041 atatggggcg atttctcaat ga
//
LOCUS       DAZUKA010000258         2043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000258 DAZUKA010000000
VERSION     DAZUKA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.264"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..629)
                     /locus_tag="ACG8PN_002953"
     CDS             complement(60..629)
                     /locus_tag="ACG8PN_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4863027.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(703..1992)
                     /locus_tag="ACG8PN_002954"
     CDS             complement(703..1992)
                     /locus_tag="ACG8PN_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863028.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          538 a          422 c          367 g          716 t
ORIGIN      
        1 tcaattcttt ggcaaggtct tcaaagcctg gcttgcctag aagtgcgaac atggttttct
       61 tatagctcat gtagaaaaat tttcttgatg atgattttat ccatcaattg agattgactg
      121 ttttgctctc ctatgatttc agctatgatt gtccgatttt gcggttttct tcttaactgg
      181 tagttgattt tcccagtttc catcaactcg ctaagcatat ccacgactcc gccaaatccg
      241 gtctttttcg taataaaacc tagtgctacc attcgttcat ctagattttt tgctgtaatc
      301 gtcacaccag taatgagcga gtgctgtaat gtttgggcca gctcttccat cccttcagca
      361 ttggatacga cggaagctgt tttgttaata ggttcttcgt ttatttcttt tggttctttc
      421 ctctccactt tcactgtttg acgaaggaca tctagttctt gcaagagcgt tttgtattgt
      481 tcttgggtca gcatcacgcc agctactttt tcacgattaa atatatatac gccagttccc
      541 gcgtctcgcg cttgagcaaa tacatccatc ggcgatcgtt tcacttctgt gatcgaagat
      601 gtgggaactt ctaatttctt taattccaat ggatttcctc ctttactaat cgctttttat
      661 tatagcacag actgacttgt tttgatcttt tttccaaagg ttttatcggt ttttgcctaa
      721 ccagtaattg atatcggtaa gaagtctttc gataccaaat gaaggatggt acagatacgt
      781 tttttgatct tcgttatggt ctagtggaaa aaaatgatcg gtgatcacta gatctgcata
      841 ccgaggcggc ttatttcgag tacatacatg tatagagatc cgttttcgat gtcttagcgt
      901 tttgttcagt accccagcaa gtcttcttgc ttcttggtgt tcatagcgaa aaaaaaagac
      961 gattttcact tcaggagggt ggacatctaa ataccctcgt tcctggagtg ttttcagtaa
     1021 aagcatcccc cactctactg cggggtgtgc aagtgcttgt tggatcgcta gccgaaattg
     1081 atatagttta tctttggaat tgggaaattt ttctaatgtt tcctgataat aacgaacttc
     1141 ttctatcagc cggtcatgga taccaattac gtaacgaagc cattcttggt aaagtacttt
     1201 tatcagcttt gatttgcttt cgctctcaaa ttcgctattc agccaaaaca gcgaatcaag
     1261 cttttcagtc acaatcgtca atttccgata gtcttgtgtc tcttctatcc gtttccaccg
     1321 cttttcagta atctggttac agctcaagcg caaaattgct agataatttt tttcttttat
     1381 tggcaaaatc tgctcctctc taaatagcaa ttcaggaaac aattcaaaga atagtgttcg
     1441 actgtctcct aaaatctcga tttctgccgg atcctttctt tgcttcaata attggatgaa
     1501 catctcagaa aaggactgct ctccatcatc tgcaaaaaaa tgcggaaaat ctttcaaaaa
     1561 gtaaaacagc agatatctta cttttctttc tgaaccaaag atctgtgctt ttttataaga
     1621 aaaagaaagg tggtactcgc caaaccactt tctcaatcgc tcttgcgtag aacgatcgta
     1681 cttctgctcg taaggttcat ttaatagcag atttttcagt aaacaaaacg ggggtgattg
     1741 gatcagaaaa acaaacaaga gataaggttc gctatatcct tgatcataaa ctaactgaac
     1801 ggttttctta tgaagaatca gctgaatggg acagtcgctt tccgccaaac gtttcagatg
     1861 ccgatccact gttttaacat cgattttcag ttcttcagct aaatcagctt ttcgccagat
     1921 caaatctttc attagattga ttagtgaaaa taaattctgt gcattttttg ataatagttt
     1981 ttgccatctc atcgatcatt tctcacctga tttctaaaca tattgtttga tcaaaatgag
     2041 ttc
//
LOCUS       DAZUKA010000259         2010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000259 DAZUKA010000000
VERSION     DAZUKA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.265"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1709
                     /locus_tag="ACG8PN_002955"
     CDS             96..1709
                     /locus_tag="ACG8PN_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4863029.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          643 a          353 c          414 g          600 t
ORIGIN      
        1 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
       61 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
      121 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
      181 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
      241 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
      301 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
      361 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
      421 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
      481 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
      541 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
      601 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
      661 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
      721 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
      781 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
      841 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
      901 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
      961 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
     1021 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
     1081 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
     1141 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
     1201 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
     1261 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
     1321 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
     1381 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
     1441 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
     1501 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
     1561 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
     1621 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
     1681 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
     1741 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
     1801 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
     1861 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
     1921 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
     1981 acgcctaata ttacgaggca tcatattaat
//
LOCUS       DAZUKA010000260         2004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000260 DAZUKA010000000
VERSION     DAZUKA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.266"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG8PN_002956"
     CDS             complement(<1..426)
                     /locus_tag="ACG8PN_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4863030.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG8PN_002957"
     CDS             complement(535..1566)
                     /locus_tag="ACG8PN_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4863031.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          619 a          328 c          347 g          710 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttga
//
LOCUS       DAZUKA010000261         2003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000261 DAZUKA010000000
VERSION     DAZUKA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.267"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..449
                     /locus_tag="ACG8PN_002958"
     CDS             <1..449
                     /locus_tag="ACG8PN_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS4863032.1"
                     /translation="DITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMPAIN
                     ILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIP
                     PHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            461..1465
                     /gene="neuB"
                     /locus_tag="ACG8PN_002959"
     CDS             461..1465
                     /gene="neuB"
                     /locus_tag="ACG8PN_002959"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS4863033.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1462..>2003
                     /gene="neuC"
                     /locus_tag="ACG8PN_002960"
     CDS             1462..>2003
                     /gene="neuC"
                     /locus_tag="ACG8PN_002960"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4863034.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTIL"
BASE COUNT          722 a          259 c          402 g          620 t
ORIGIN      
        1 aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata ccggatgctg
       61 caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat gtcttaaaaa
      121 tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat aatgatacac
      181 atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga gaaaaattat
      241 atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat aaaatgtata
      301 tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa aaaattaaaa
      361 tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt attagaatcg
      421 caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag aagtatatgt
      481 aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag aaagtattga
      541 taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa cgaataagct
      601 agtttccaaa aaggctccaa tggcaaaata tcagaaggat aatcttaaag aggacagttc
      661 tcaatttgaa atgctaaaaa aattagaatt atctcaatct gattttatta gaataaaaga
      721 ttattgtata caaaataaaa ttgatttttt gtctactcct tttgatgaag aaagtgcaga
      781 ctttctaaaa caaattggtg tacaaggatt taaaattggc tcaggagatt taacaaattt
      841 accttttctt agaaaaatag atatgtatgg gttgccagtt ttattatcta caggtatggc
      901 tgatattcat gaggtcagtg aggctgtaga ttgtttcata gatagtccag ttactatttt
      961 acattgtact tcaaattatc cggctagttc tgaagatatt aatttattgg cgattaacac
     1021 attaaaagaa atgttcggga tacccgttgg gtactccgat cattcattgg gatatgatgt
     1081 tggtatttgt gctgttgcga tgggtgctaa ggtaattgag aaacatttta ccttggataa
     1141 ggaactacct ggtccagatc ataaagcttc tttagatcct caggaatttg cagattttgt
     1201 aaagcatatt agaaacactg aaatattttt aggtgatggt gttaagcgag caatgccttc
     1261 tgaacttagt acaaaagaag tatcaagaaa aagcctagtg gtcactgaga atttaaatgt
     1321 gggagatatt cttacagaga aatctcttac aataaaacgt cctggaaatg gaatagctcc
     1381 taagttttta tatcaatata ttggcaaaaa agttaaaaga ccaataagtg cagatagtgt
     1441 tctgcttgag gaagatgttg aatgaaaaaa atacttatag taagtggaac acgagctgat
     1501 tatggaatat ataaacctgt tgctgagaat ttactacaag ataattggga tgtatcattt
     1561 gttgtttctg gaatgcattt atcccataaa tatggattta ctaaagaatt gatcttagct
     1621 gacggattta gaattagtgg agaagttcaa agtttgtttt tagagaacaa taagggaaac
     1681 atggctcggt ctgtggcatt agagataaat ggtttcacaa atatatttga acaaactagc
     1741 cctgattttg tattattact aggggataga ggagaaatgt tggcagctgc gatagcttgt
     1801 ttgtatcttg gaatcaaaac agcacatatt cacggaggcg aagtgtctgg tactgtagat
     1861 gaatcaataa gacatgccat aacgaaaata gcatctgttc attttgctgc tacggaaaaa
     1921 agcaaagagc gtttaataaa aatgggggaa gattcttgga gagtttttcc cgtcggagct
     1981 cctagaatag atactatttt aaa
//
LOCUS       DAZUKA010000262         1932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000262 DAZUKA010000000
VERSION     DAZUKA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.268"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..802
                     /locus_tag="ACG8PN_002961"
     CDS             <1..802
                     /locus_tag="ACG8PN_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4863035.1"
                     /translation="SLFESEGFDIVIHLAAQAGVRYSIENPYAYIDSNIIGFLNILEA
                     CRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSNELMAHTYSHLYK
                     IPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERDFTYIDDIVEGIT
                     RLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVETIEKYLGVKANKI
                     FLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYYLS"
     gene            1170..>1932
                     /locus_tag="ACG8PN_002962"
     CDS             1170..>1932
                     /locus_tag="ACG8PN_002962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863036.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFA"
BASE COUNT          623 a          276 c          350 g          683 t
ORIGIN      
        1 ttctctattt gaaagtgaag gttttgacat agtaattcat ttagctgctc aagcaggtgt
       61 tagatattct attgaaaacc cttatgctta tattgattct aatattatcg gttttcttaa
      121 tattcttgaa gcctgccgaa acttccctgt aaagcatcta ttatatgcat catctagttc
      181 agtatatggt gggaacacga aagtaccgtt ttcaacagaa gattcggtgg atcatccagt
      241 tagcctttat gcggctacca aaaaatcgaa tgaattaatg gctcatactt atagccacct
      301 ttataaaata ccaacaactg ggctgagatt tttcactgtc tatggtccat ttgggcgacc
      361 agatatggcc tatttctctt ttaccaagaa tattttatct ggaaaagaaa ttaagatttt
      421 taaccatgga aagatggaaa gagatttcac ttacatcgat gatatcgtag agggaatcac
      481 tagattaatc aataaagtac ctgaacccaa ctccgactgg acagacatcg atgatttgag
      541 tagtgaaagt tgggcaccat ataagatata caatattgga aataataagc cggttgcatt
      601 gttggagttt gttgaaacaa ttgaaaagta tttaggtgtt aaagcgaaca agattttttt
      661 ggacatgcag ccgggtgatg tcgttagaac ttatgccgat attgaagatt tagaggaaaa
      721 aatcaacttt aaaccgaata taaccttgga tgagggcatt aaaaaattcg ttgaatggta
      781 tacagtgtat tatttgagtt aattttgaaa ggaatattga agatgggtaa ttcatacgat
      841 ttttttagct ttgttaggta ttttaggaaa ttctactttc aaacgtgacg aactttctcg
      901 ccgaatagat attttctata actaaaagaa tttctataaa acaaaattgt gtatcaagat
      961 gtgtgaactg gattctgact gccaatattg attgatgaga ttgaagaaat atatagttga
     1021 tcatggatat ctttatttga aaagatctta gctattcaca tattcaaaaa aatttttact
     1081 acgaggactc acacgataca gaggaattta ggtagttcta attaaaccag aaagtcatct
     1141 atagatgtga gttgagggag gtcatacggt tggatagtag cagtaataaa tcacattcaa
     1201 tagctaaaaa agcttttatt tacgcaatag gaacattatc atcaaaaatg atatctgtca
     1261 ttataatccc tatctatgca gtatatattt ctgttgccga tttaggaagc tttgattatc
     1321 aaaatacaat tgctcaattt ctttctccag ttatttttgg agcaatatgg gaagcaattt
     1381 tacgcatggg attaggcaaa gaaaaaaata ttcagaaaaa agtagtgacg aacagtattt
     1441 gttttgttat agcaaataca gtgatattag ccattttttt accaataata tatagaatga
     1501 ttttcccgaa tttagagtat tatcaattgt ttgtttttca attaattttc actcctatcc
     1561 taagtgtttt tcaatatatt gtaagagcac taggtgaatc aaagattttt gtgatgagtg
     1621 gtataatcgc tgcgggtatc aacctgttac tccttactat ttttgttgtt acaatgggta
     1681 tggggatact tggtctctta ctctctatca taatttctaa tgttataaat ttgatttttt
     1741 tgattttcag ggggcagatc tttcaatatt tcgcaacgag ttcttttgat tttttatttt
     1801 taaagtccat gctgaaatac tctatcccac tcactttgaa tctcactttt ggatggctaa
     1861 tagttggttt ttcaagattt tatattggaa cgtacatcgg agtaacagaa aatggtattt
     1921 tttcttttgc ga
//
LOCUS       DAZUKA010000263         1927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000263 DAZUKA010000000
VERSION     DAZUKA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.269"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1377
                     /locus_tag="ACG8PN_002963"
     CDS             <1..1377
                     /locus_tag="ACG8PN_002963"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4863037.1"
                     /translation="EWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQH
                     SAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPR
                     LVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKH
                     KVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENL
                     TLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVK
                     VTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRA
                     VRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYG
                     KYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIA
                     FPNEKIFR"
     gene            1393..>1927
                     /locus_tag="ACG8PN_002964"
     CDS             1393..>1927
                     /locus_tag="ACG8PN_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4863038.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKN"
BASE COUNT          652 a          318 c          355 g          602 t
ORIGIN      
        1 gaatggagtg ctgcacgacg gatattcatt atattaatgg atgttattgt atacaatgca
       61 gcaatatata gtagtttctt actgaagttt cgtggagaga tcccattacg aaatttagaa
      121 actttccaac actcagcaat tattatttca gtgattttta ttgcgttgaa cattttgttg
      181 ggtgcatatg ttttttataa tcgaatgatc agtgacatcg tgtttgtcac agtcatcggt
      241 caggtattga tgacactagg gatcatggta gtgacttttg cgggccgctg gtttgctttt
      301 ccacgtcttg ttatcttact ttcatttgtt ctcggtacgc ttttcttaag tatctatcgt
      361 attctcgtat ataaacttta tttacgcgta agtcgagata aaaaggtcgt cattattggg
      421 ttagaaaagg atgtcgcacc agcaatccaa aattttggtt caaagaaaaa caacaaacat
      481 aaagtcgaag cagtcattct tgataactat tatgcaaaca tcaaaaatat gattaaagac
      541 atcgatattg tctatcttgc ttctcatatc gaagaaactg aaaaaatcaa aatctatcaa
      601 ttagttacta aaaatgaaaa aaaattgttt ttgaatacaa catttgaaaa cttgacattg
      661 atcaatccaa atattatgaa tttcgaagat gaaagtatta ttgaagcatc tggatttaga
      721 atccaacctg aatatgaact attcaaacgt ttgtttgata tcatcgtttc acttgtttta
      781 ttagttattg cttcaccatt tatgttattc acagcaattt tagtcaaagt gacatcaccg
      841 ggaccaatca tctataaaca agtgcggatc acaaaaaatc aaaaggaatt ttcaatctat
      901 aaattccgct cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt agctaagagc
      961 aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt tgatgaatta
     1021 ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg tcctgaacgt
     1081 cctttcttcg tcgatcagtt caatgcagaa aacccttatt actacttacg gcataatgtt
     1141 cgtgctggta tcactgggta tgcacaagtc tatggaaaat atgtctctga ttacaacagt
     1201 aaattacgtt ttgatttact ttatatcaaa aaatattcat tgatgcttga tttaaagatc
     1261 ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct ctcaaggact tgaagaagac
     1321 gaaaatgtga tctcattggc agaaattgct tttccgaatg aaaaaatctt tcgctagttg
     1381 ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg taccagtata caatgcagaa
     1441 gcaactattg gtcgaacgat tgatattcta aaagaattgg attatgaaga gtacgaagtc
     1501 gttttagtaa atgatggatc aacagatcaa acacaggaaa tcattcaaga actgattgaa
     1561 aatgatgaac gttttgaatt gattactacg accaatcaag ggcccggatt agcaagaaac
     1621 gaaggaataa gacagtcaaa aggagaatat ctcttattct tcgatgcaga tgacacgcca
     1681 gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa agcggatctt
     1741 gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc cgaaaaagaa
     1801 aacttagtta cagaatgtca ctatctaact cacgaagagt ttataaaaga catgtatcag
     1861 ttaatgaatg aacagttgat gtatgttgta tggaacaaat gctatcgtag cgatattctg
     1921 aaaaata
//
LOCUS       DAZUKA010000264         1924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000264 DAZUKA010000000
VERSION     DAZUKA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.270"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8PN_002965"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8PN_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4863039.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8PN_002966"
     CDS             852..1040
                     /locus_tag="ACG8PN_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4863040.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8PN_002967"
     CDS             1053..1610
                     /locus_tag="ACG8PN_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4863041.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8PN_002968"
     CDS             1642..1884
                     /locus_tag="ACG8PN_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4863042.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          709 a          296 c          395 g          524 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaat
//
LOCUS       DAZUKA010000265         1859 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000265 DAZUKA010000000
VERSION     DAZUKA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.271"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACG8PN_002969"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACG8PN_002969"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4863043.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAZUKA010000266         1833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000266 DAZUKA010000000
VERSION     DAZUKA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.272"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..424
                     /locus_tag="ACG8PN_002970"
     CDS             227..424
                     /locus_tag="ACG8PN_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863044.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            530..1012
                     /locus_tag="ACG8PN_002971"
     CDS             530..1012
                     /locus_tag="ACG8PN_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863045.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1070..1666)
                     /locus_tag="ACG8PN_002972"
     CDS             complement(1070..1666)
                     /locus_tag="ACG8PN_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4863046.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          577 a          271 c          364 g          621 t
ORIGIN      
        1 tatattttaa tatgtaggaa ataattagtt tttaaggaat atgttttaat tttttgaaaa
       61 aaattcttct aaatacaaca gctgttttaa ttgtgaaaac gaaaacagta cgctataatg
      121 cctgtaaggt tgatttttaa ctgtaaataa aaatcaatga gttaaatctc caaagaaata
      181 gcagtggtaa tgtggagaaa agttccagca gtttaggagg attttgatga cggaagaaat
      241 gattaattta ggagaacaat atgcatgcaa accaattggt tttacaaaaa cggtcattgg
      301 tgaagttgta tcaaaaatga caaactgcgc cgtggtcaaa gttgcccaat gtgctgcaga
      361 ggaccaagaa ttattagatg aaaaagcaag catggttgtt gcaaaatatg atacatttga
      421 ataaagaaaa caattggacg tgcggcgtgc tgcaagtcca atttctttga gaaggtaaat
      481 cagtattctt ttcctatttt ctggagaaga aaaagcgagg cgagttgtca tggtaacatt
      541 ctttacggtt ctattgtttt tattcgttgt tgtgatgttt ttatttcttt ggctggttcg
      601 aaaagaaatt atttatcgga ctgtgcgaaa tcgatgggtc tacttggtga tacccttctt
      661 agtgtcacta gtcatttggt atacactcat cagtcatcct acagcagatg aattggcaaa
      721 agggatcttg tcagcaatga tttttattag ttttttgttg gacagtagag gaatcacaga
      781 agaaggactt gttttgaata gctttgacaa aaaaggtgtt cctttttctg agatcaataa
      841 aattgtatta tatcagccaa aaggttctaa aatcgtaaaa atgaattttt tccgaaacgg
      901 atggcgagga ccgatgctga aattttctgc atcattagaa gaattagttc catttttgtc
      961 ccaacgctta aacgatgagg cagagattga tattatgatt gatccagaat aaaaacagag
     1021 gttgcgacaa gatgtttgtc acaacctctg ttttttattt caaataaaat tatccaaata
     1081 cgcggaagcg atcggcatcg ttcatgtatt ctttttcctc agccggtgtt tctggtgaac
     1141 cgaatactaa ttgcgaacgt agtttccagt ttgaagggat attccattct tttgcaactg
     1201 cttcgtcaat aactgggttg taatgttgca agtttgcgcc caatccttta tcaactaatg
     1261 ctacccaagt attagctgtt gcgattccgt ttgattgttc tgaccaatct gggaagttgt
     1321 ctgcatataa ttcaaattgt tcttgtagac cttttaccac gtcagtatct ttgaagaata
     1381 atactgtacc ataaccattt ttgaaaccag ctaatttgtt ttgtgtattt gggaaagctt
     1441 ctgctggagt caatgggcga agtgcttctt cagtgatttc ccaaagtttt tcatgtgcat
     1501 ctccgaaaag gataactgcg cgtgttgatt gagcattgaa agcagttggg ctttctttaa
     1561 ttgcttcttt gatcaaagtt gttaattctt cattagaaag tgaaacatta cgtcctagat
     1621 gatagattga acggcggttt ttcaaagtgt cagtaaaagt tgtcataata aaatttctcc
     1681 tttgatttgc gttttgcatt tttttaattg attacgaaat gaagtatagc accttacaaa
     1741 aagtaagtaa acagatatgc tcacaaaaaa taagcgaagt tcaaaaaaca attttaataa
     1801 gaaaaatcgt tatgtaagga ttttcttagt tat
//
LOCUS       DAZUKA010000267         1790 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000267 DAZUKA010000000
VERSION     DAZUKA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.273"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..961)
                     /locus_tag="ACG8PN_002973"
     CDS             complement(<1..961)
                     /locus_tag="ACG8PN_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS4863047.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQA"
     gene            complement(1120..1386)
                     /locus_tag="ACG8PN_002974"
     CDS             complement(1120..1386)
                     /locus_tag="ACG8PN_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4863048.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1376..1732)
                     /locus_tag="ACG8PN_002975"
     CDS             complement(1376..1732)
                     /locus_tag="ACG8PN_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4863049.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          516 a          340 c          214 g          720 t
ORIGIN      
        1 cagcctgtat tggagctttg tatttctcca taataacaga tatctttttt tcatttttta
       61 aaaaatacct tttaaatttc ttatagtcct ctgaaatcaa aatatagcgt gatggatact
      121 catcaaaaga agaatcgtat tcttccttta aaattcccga tttacttaat ttttctagta
      181 catcaaatac tctatcactc aagtaactcc ttaaattgtt atcactttcc cattgaacta
      241 cttttctata agttgaatta tcagttgaaa agatagaatt ttcatccata aacgcatata
      301 ttagcagcaa tctttcatca ttgtcaaatt ctattggatc aaaaatatct aagttgtcat
      361 caaaatcttt atcagttccg gtaacttttt tgttaattac tgaaggtgtc ttgtaagaaa
      421 tagaatctaa atcaaagcgt tttgattcta aattattttg tgctttctca attaaataat
      481 tcactttttt tgtgagaaac cttctgatat tgtctttgct atatgttcca taaaaaccct
      541 tatcttctaa agagtccctg tacttcttaa cttctatata ctcttcagcc aaaacttgtg
      601 gaactggttc atctaagaaa aaaaccccta cctcgccttt gctattttta taaaagtaat
      661 caatttctgc tatagttccc gatctataat tatcaactgg agaacccaac ttatggccaa
      721 aaatcgctaa taatatatca ctattttcta caatttgttt attaataaca ctttgcccag
      781 agctattatt tatataagta ggaatcgcat tcgtttccca tcgaattggc atcaaaatta
      841 cttttgtatt atctgtattt tctatattcc atctatatat ggtttcttct acttcattcc
      901 tctttgctgc tatatcacct ggtgatgcta taaatacttt tattagattt atggttttca
      961 tttattgtac ctttctcttt atgtctcttt aaagtaattt ttgtataaaa tttattgact
     1021 tttacactat aggacaactt ttatctattt agtacaaaaa ggcccactaa actaattgat
     1081 tagttaggag cctattacag gtatattttc aactcgcttt taagcaaatt ctactaccag
     1141 ctttttgcct aatgccctag cgatatcatc aagtttatcg aaagatacgt tatcccctct
     1201 ctcaattcga gcaatagtag attgagttgt atgtgctttt tgtgcaagct ccgcttgagt
     1261 taggcctgct tcctcccgtg catgataaag agcaaccgca gtttcgagtt ttcttccttc
     1321 acggtcatat gcttctgcaa aatctttgtc tttttgcttt tcttcaatca attttttaat
     1381 catcatttgt gaaccactcc tctctaagtt cttttgcatg tttaagttca cttattggtg
     1441 ttttctccgt ttttttagta aatccatggg taatgacata ccttccattg accacatgga
     1501 aatagatagc tctttggatg tttgatccta ccttacttct taattcgtac aaattagtgt
     1561 ctaatttttt cacccatttc aaccgttgag ctactagcat cccattcttt tctgtttctt
     1621 ctattgtacg taaaagcttc gcactatctt tttttggtaa tgtttccaac cattctaaaa
     1681 attcatcatg accattaggt cttttaaatg tttcaaaaga tattttttcc atacccaatt
     1741 atatcatata ttgattttat attatagtat ataacataaa aattcgaatt
//
LOCUS       DAZUKA010000268         1763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000268 DAZUKA010000000
VERSION     DAZUKA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.274"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1089)
                     /locus_tag="ACG8PN_002976"
     CDS             complement(85..1089)
                     /locus_tag="ACG8PN_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4863050.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1160..1456)
                     /locus_tag="ACG8PN_002977"
     CDS             complement(1160..1456)
                     /locus_tag="ACG8PN_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863051.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1449..1742)
                     /locus_tag="ACG8PN_002978"
     CDS             complement(1449..1742)
                     /locus_tag="ACG8PN_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS4863052.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
BASE COUNT          498 a          343 c          235 g          687 t
ORIGIN      
        1 ttttacttta taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt
       61 ttctttgata aataatattt tcaattattt aatcatttga aaattgttag tcattccaat
      121 acgttgagaa attcttccat ctgtataaac acggaattgt tttccaccag ccattgttgt
      181 atttccatta tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat
      241 tagatctttc atatattcat ttgtgctaat taaattagta tttgatgcat ttttatcagg
      301 agaatcatac aaccataaag ctttattatt tagtacagtt tctttagcag cttcccaact
      361 tgaaattgct gagctacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc
      421 ttctactact ggagtttgag gagtttgtcc attcacaata tcaatgaaac cttgttttaa
      481 ctgttggttc tgagtttcat tgaagtacat gtagttttta tcattgcctt gattttgttg
      541 cgttaaataa actttaggtt gtccattttg aaagccaaag aaatatacat gctgccctaa
      601 atattctcca gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt
      661 tccatcttct gaccattgta cggtaactgg tgcttcattg attgccattt tccaatttcc
      721 atcaataact gcacttggta aaggcgtacc atacagactt acattgttct gaggattgta
      781 gcttttatat tcttgaccta atgtctttcc ccattgtggg acaaaaattg ctagttgagt
      841 agatttctca ctatcccaca aagtattttt aacctcttca gaagaagatt caatgattga
      901 actttcactt gtacttgctt ccacagattg tttcatttct gttgtagttt gttcagttga
      961 ttgcgtttta ctttttttag cctcaattgt tgtatcagtt tctacagtct ctttagtcct
     1021 cgtattctca gatgtacaac cagtgaataa aatccccatt ccaattagta ataacaacca
     1081 ttttttcatt ttatttcccc ctttcaattt ctccctcgcg tacttatttt atcagtcaat
     1141 taatctcaat ggtgttcatt tagttaatcc tttcccaaga atgtcgatag ccagcatgac
     1201 aacgatctct tccttgaggg ctttcagatg gattaaaact agtagaaatt tcccttccac
     1261 atcctctaca gcgataagtt ctcattattt ttgggctatt agtattattt ttcttagtta
     1321 gagctatgag tccaactatg attaccccaa ctgtcttaat tacttttcct ttatctatat
     1381 tcggatgcgc aagcatatat ttatataacc aatatccacc gtaaattaaa ccacctaata
     1441 caatcagctt agccatgtac tcgtctcctt tctttttgtt taattacttt ttgaatccat
     1501 cttgcagaga aaccataaac ttctgtcaat tgacggatat ctacagtttt ttcctgttct
     1561 atcatgtttt cgattctttt tctaactaac tcacgatcgt acattttcat tggaaaagaa
     1621 atctgttgcc ctcgaaaagc aagataaaat tgatagagca attctgaatc cagttccatt
     1681 tcctcaaaaa agtcattcat acttttataa gagttactca aacaagtttc atcaatctcc
     1741 atctacacac cccctgttat ttt
//
LOCUS       DAZUKA010000269         1760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000269 DAZUKA010000000
VERSION     DAZUKA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.275"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8PN_002979"
     CDS             278..820
                     /locus_tag="ACG8PN_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863053.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACG8PN_002980"
     CDS             976..1425
                     /locus_tag="ACG8PN_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4863054.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>1760)
                     /locus_tag="ACG8PN_002981"
     CDS             complement(1455..>1760)
                     /locus_tag="ACG8PN_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4863055.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          655 a          256 c          334 g          515 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt
//
LOCUS       DAZUKA010000270         1740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000270 DAZUKA010000000
VERSION     DAZUKA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.276"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..864
                     /locus_tag="ACG8PN_002982"
     CDS             64..864
                     /locus_tag="ACG8PN_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863056.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            970..>1740
                     /locus_tag="ACG8PN_002983"
     CDS             970..>1740
                     /locus_tag="ACG8PN_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863057.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLID"
BASE COUNT          677 a          210 c          371 g          482 t
ORIGIN      
        1 gtagaaaatc ttatttgtga atgaaaaaca agtcatcgta aaatattaat aggaggtgta
       61 gtaatggtgg gaatatcaaa aacagcttta gactcattag ctaaactatt tagtagcagg
      121 tttgatggtg cagaatttgt tgaatatcta agccaaaacg aaagtggaaa aactctgatc
      181 gaaacaatga aaaaagacta tttgaatgaa aatgactttc cgacatggac gaatattgaa
      241 gatgctacat gggatgtagt atgtacgata tattctcctt gggtaacgat tgctgatttt
      301 gttttagata aaattggagg ggatacatac cctgatgcca aatctcttgc tacaagtgac
      361 tttaaaacgt tctatggtta tctaataggt ctaataggca atggaataga tttgaaagtt
      421 acgccaaaac aaacacctga agaaattgaa aaagaaaaag gagaggttca aatggcttat
      481 gcaaatgaca gagaaaaagt aaaattattg gaaggtaaat tatctgaaga agaacttgga
      541 aaaatggtgc aagaaatcaa tgaagacagc aactattttc ctgaattgaa ggtatatcaa
      601 aatgacgaag atttctttaa tgaaatgttt agtaatagtc catgggaagc agtgagtaaa
      661 acaaatttag gagaatggaa ctttcaagat gatctagttg catttagcaa tgatgggtat
      721 ttacgatcat attcagaaag tgagtataga gctgaattaa aaacgtatga aacggatatt
      781 ctgatgagct atttaaatct attagagagt ggtcagggag agttaagaga taaagttgaa
      841 gaactattaa aagaagagct atagggatta tttcaacata aacacaagca gaaaactact
      901 aaacaaaacg taccatgcaa aaagtcggtt aaaccggctt tttgctctat tatgagagga
      961 gatcaaacca tgagtgcaac aaccgatatg gctttagaag cagtaataaa agataataca
     1021 ggaaatgtcg aaaacgtaga agactttagc gatattgcaa tcaatctttc agcatggatt
     1081 tcagctgtag gagaagaaaa tatcgaattt gagatcaaga atatggatga cttaaaagca
     1141 aaatttcctt ttatcgcatc gaaactaagt caagttgccg aggataaatc gtggaatgaa
     1201 ttagttagtt tttccgaaaa aatttatgat attacgcaag aagatgagtg tgtttcatgg
     1261 gaaatgaata atcaatacaa gaaaattgac gctttcgtca aatcacgtat agaaggaaca
     1321 gcttttgagt ttgaaggtat agataccgca gataattggt taaataaaga atcctttatt
     1381 aaaagtatag cggatgatct tgaaaaacaa tggtctaagg aagactatgc agagtaccta
     1441 aaaacagaaa gagatacaga ggtgaaagaa aaactcactg aggtatgttt ggatgaacat
     1501 attgatccca atatcgtaaa acaagaagac tatgaagtgg ttattgataa agttcctact
     1561 gattttgagg aagttgtaaa gtatctagtt gaagaagcat tatcgcaata ctcttcagat
     1621 gaggaagtgg acgaagaaac tgttcgtacc agagtttggc aactggtttt tgatgacaaa
     1681 gattataaat atttgagtga tcaaaactcg gttaattgga gtgtacgatt attaattgat
//
LOCUS       DAZUKA010000271         1738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000271 DAZUKA010000000
VERSION     DAZUKA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.277"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..687
                     /locus_tag="ACG8PN_002984"
     CDS             331..687
                     /locus_tag="ACG8PN_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4863058.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            697..1596
                     /locus_tag="ACG8PN_002985"
     CDS             697..1596
                     /locus_tag="ACG8PN_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4863059.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          580 a          293 c          353 g          512 t
ORIGIN      
        1 tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct
       61 ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag
      121 gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa
      181 cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt
      241 actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa
      301 gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa
      361 gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt
      421 gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc
      481 attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca
      541 gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt
      601 gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc
      661 aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc
      721 gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt
      781 ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac
      841 cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc
      901 ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca
      961 cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat
     1021 ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag
     1081 cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca
     1141 gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg
     1201 attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat
     1261 gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac
     1321 cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa
     1381 tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga atggcagctg
     1441 acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt
     1501 gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag
     1561 gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag
     1621 aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg
     1681 ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaact
//
LOCUS       DAZUKA010000272         1738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000272 DAZUKA010000000
VERSION     DAZUKA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.278"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..188)
                     /locus_tag="ACG8PN_002986"
     CDS             complement(<1..188)
                     /locus_tag="ACG8PN_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4863060.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIK"
     gene            complement(185..838)
                     /locus_tag="ACG8PN_002987"
     CDS             complement(185..838)
                     /locus_tag="ACG8PN_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4863061.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1126..>1738
                     /locus_tag="ACG8PN_002988"
     CDS             1126..>1738
                     /locus_tag="ACG8PN_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4863062.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          554 a          315 c          234 g          635 t
ORIGIN      
        1 tattttattt cttttccatt catattacta gaaatttcat ttgccatatt atgaatcttt
       61 tcttttctcg tctccaaata tgttttagga aagataaaaa aaattaatga atgaactaat
      121 ataattatta ttccaagaac agaaaatatt tgtatgaaca tttttggaaa tatctttaat
      181 tttttcattt tctctccaat ttatatccta catttcttat agtagttata cagtctaatt
      241 gtaatttttt tctaagttct tttatatata catctattac tcgatcataa ggagtttctt
      301 cgctatcttt ccatacataa tcaataattt gcattcttgt taacacttgt ccattattat
      361 ccaataaaca ttttaatact tctagttctt ttgcatttac atctatttct tcaccattta
      421 ttttagctgt atagctatta aaattaaccg atagatcctt atattcaaac ttctctaaat
      481 gtccataatt cttcttaatt aaagaatcta ttctagcttt taagaccggc aatgaaaatg
      541 gcttttctac atatccgtct actaaattag taaatgcatc aattttatat tcttcatcac
      601 taaatgcagt caagattaga attggtaaat tgcttttctt tctaatttct ttcaacactt
      661 ctaaaccatt tataaaaggt atctgaatgt ctaagataac caaatttata tcgctattaa
      721 attttgacaa ggcttctctt ccatctttag cttgaataac agtatatcca aattctgaaa
      781 gatattcact tattccctct cttatcatat tatcatcttc aacagttaat attttcatat
      841 atacctccac ttaagcctat acactagata ttatacagca tattatggct ctataatatc
      901 tagtgttggt gacaccttct gttgccaata ctttttcttt tttctaataa atgcaaacag
      961 acaccttgaa atcctttaga ataaagggtt ctataatatt ttgtgttgtt ggaaaatcga
     1021 aggcgatttt ctactaacac tttttcttcg tttcaactca aaaagagact cacttttggt
     1081 aaaatttagt cgaccaaaac atcaccctac caaaggagtc gctctatgga taatcatact
     1141 agaaaattac tcgtattaac agataaacat ttattttttg atgaagaatg gctgattgaa
     1201 aaagaatata aaggcgttca agcgcagttt attaaaggga gattggatga ctcaccttcc
     1261 cattgtgaac actgtggatc gattcgtatt atccgcaacg gttcctatac gacacgtact
     1321 cagatattga aagtcaaaga aaagttgact attttagaat taaaacgtac ccgttttcta
     1381 tgctatgatt gtgggcagac cttttctgcg aaaacggact tagttgatga acatcatcaa
     1441 ctaacaaagg aactgaagca ggcgattctc atagaactgt acgaaaatca atcacgtaaa
     1501 ctaatcgcta agaagtactt cgtctcggat gggacggtga cacggatctt gcgtgaagca
     1561 acgaagcatt accagccacg tatgaacttc cttccgacgg tgttgtgtat ggatgagttc
     1621 aaatcaatga agtctgtttc tggttccatg agttttattt gtgttgatgg gacaacccat
     1681 cagctattta cgatccttga agatcgcaga ctttacaaac taactcaata cttcctgc
//
LOCUS       DAZUKA010000273         1730 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000273 DAZUKA010000000
VERSION     DAZUKA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.279"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..379
                     /locus_tag="ACG8PN_002989"
     CDS             2..379
                     /locus_tag="ACG8PN_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863063.1"
                     /translation="MTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKRKLSHG
                     YFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGNICPGF
                     MDQIIENESIESIENYVPVDLPK"
     gene            complement(552..755)
                     /locus_tag="ACG8PN_002990"
                     /pseudo
     CDS             complement(552..755)
                     /locus_tag="ACG8PN_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            721..918
                     /locus_tag="ACG8PN_002991"
     CDS             721..918
                     /locus_tag="ACG8PN_002991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863064.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(961..1536)
                     /locus_tag="ACG8PN_002992"
     CDS             complement(961..1536)
                     /locus_tag="ACG8PN_002992"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4863065.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
BASE COUNT          582 a          291 c          240 g          617 t
ORIGIN      
        1 attgacgtgt tctaattgtg gtacattatt ttactatgag gaagttagaa atattgtaaa
       61 acatagaaaa aaagaaatgc ctatacgttg tccaaattgc aatgaagtta ttaagcgaaa
      121 gttgtctcat ggttattttg tcagctacga actatatgaa gaaagtaaaa taaatctgaa
      181 aaaaaaacca aaatactata aaaatttgaa aactggagct attataactg aaaaagaatt
      241 taaaaattta gtgactgaag aagaaagcag gttttataat ttgtctggta atatttgccc
      301 tggatttatg gatcagatta ttgaaaatga gtcaattgaa tcaattgaaa actatgttcc
      361 tgttgactta ccaaaataga aaaaataatc ctgtttttac agtgaattat tttaattttg
      421 attttcaata aaaatatatt acaataataa tcgtaatagc taatgaaaca atttctcaat
      481 ctttttacca ctccaaacag ggagcgtcaa taattttgtg taaataactc gtccttctgc
      541 aaaataatgg gttactcagt aaacattgaa gctaatgtat ccgtcacttg ttggaagcct
      601 ttatggcttc tatttaagaa tttttgattg tatgtatcaa agatggatac taggaaacgc
      661 tctagtgatt cttcattttg aaactgctct tttctgcggc tgtatttctt gatttgttta
      721 ttgaaagatt cgattgtaga accagaagga atggagaaca cttttgaact ttacaacaaa
      781 actactgttg ataaattaat tccaatttta gttccaactg atttaaatgg tcatgatgtt
      841 ttacttcgtt ttaacggatt tttaggtgag gattatagat acctaaaaat ttctgatcct
      901 caacctttaa cagaataata aaaaacgtgt aatcaattat ttgattacac gttttttatt
      961 tcaatcttcg ttgtagtagt ggttgcgaca taccgaaagg taatcttcat tacctcctac
     1021 tacaatctgt tcacctgaat aaactggaat accatcaaca atacgagcat tcattacagc
     1081 ctttttattg caaaaatggc aaatagtttt aatttcctca attttatcag caaatgtcaa
     1141 taatgcttcg gatcccttga agagattatt ttgaaaatca ttcttcaatc catagcaaat
     1201 tactggtact tcctcattca ttacgataag tgatagatca ataatattct ctctacttaa
     1261 aaattgagac tcatcgacca aaatacaatc aacttttccg tactgatcaa taatctccac
     1321 taattctccg tacacatcca tttcagaagt aattgcaata gctggtttcg aaacacctat
     1381 acgactctca acttttccaa atcctgatcg agtatctatt gcgcttgtca ttatgataac
     1441 tctcttccct tgttcttcat aattatgagc cactttcagt agctcaatac tctttccact
     1501 attcattgtt ccatatctgt aaaataattg tgccattaaa tttattccct tcttttacaa
     1561 ttcaattatt ccttaattcc atctgctttt atgcagctgc tcgtttattc cttgatatgt
     1621 ctataacatc tactatccct aaaaccaatt tataagccat tagagatggt attcctgaac
     1681 atctctcata ttgttttttg agtggtatat catctgaaaa actgaaggaa
//
LOCUS       DAZUKA010000274         1649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000274 DAZUKA010000000
VERSION     DAZUKA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.280"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1604
                     /locus_tag="ACG8PN_002993"
     CDS             138..1604
                     /locus_tag="ACG8PN_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4863066.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctg
//
LOCUS       DAZUKA010000275         1625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000275 DAZUKA010000000
VERSION     DAZUKA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.281"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..400)
                     /locus_tag="ACG8PN_002994"
     CDS             complement(164..400)
                     /locus_tag="ACG8PN_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863067.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            498..677
                     /locus_tag="ACG8PN_002995"
     CDS             498..677
                     /locus_tag="ACG8PN_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863068.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            808..1077
                     /locus_tag="ACG8PN_002996"
     CDS             808..1077
                     /locus_tag="ACG8PN_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4863069.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1070..1327
                     /locus_tag="ACG8PN_002997"
     CDS             1070..1327
                     /locus_tag="ACG8PN_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4863070.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          596 a          226 c          317 g          486 t
ORIGIN      
        1 acgactttaa ctgatttttt agacacctat acttacgacg atacagaaga aatccaaaat
       61 gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaaag gcaataatca
      121 tttataccga aaagtgagaa tgtgtgaaat aaggacaaaa aacctagtaa ctaagggaat
      181 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
      241 cgggcaacac ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
      301 tattgttagt cctatttgtt ggcgaaatac ttatgcgcca tgctgcagct gtgcatttaa
      361 acttcgtagt atttttggca atactggtat tttgtttgat cttttggcgc tttgtattag
      421 cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg ttatttcgat
      481 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gttacgttgg
      541 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggcaaa agtgaatcca
      601 ggattatgtg tattgcgtta gatttattga ttgcccaaga aaaagcagga tttgaggtac
      661 cagcactcaa aaagtgacgt cactttttat cctaaaaact aaaagtgata gcacttttaa
      721 ttataagaag ttagaatatt aatcatttgc ttaattgtac aatataatgt acaattgttt
      781 tatagaaata aataaggggt gaaaggaatg gaagcagtag cttattcaaa tttccgccaa
      841 aatttacgta gttatatgaa acaagttaat gaggatgctg aaacacttat tgtaacaagt
      901 aaagatgtag aagatacagt tgttgtatta tcaaaaagag attatgattc tatgcaagaa
      961 acgttgagaa cactttctaa taattacgtc atggaaaaaa ttcgtcgagg agatgaacaa
     1021 ttctccaaag gtgcatttaa aacacatgac ttaatcgagg ttgaatctga tgattaaggc
     1081 ttggtctgat gatgcttggg atgattatct ttattggcat gagcaaggaa acaaaagcaa
     1141 tataaaaaag attaacaagt taataaaaga tatcgatcgt tccccctttg ctggattagg
     1201 aaaacctgag ccattaaagc atgatttatc tggaaaatgg tccagaagaa ttacagatga
     1261 acatagactg atatatagag ttgaaaatga aacgatattt atttattctg caaaagatca
     1321 ctattaacca atcggaagta aggaaagggt cagaaactta aaagtttttg atccttattt
     1381 tatttaccct agtcatttaa aaagctaata tagcttagtg ttgattgtta ttaatgaatg
     1441 tgtttgttac gcgtattacg gatataaggt tagtaaaatc atttctaaag ttgaggaaaa
     1501 gtaaatataa atggcttaaa tttcaacaat ttgaagttga atagatatgt tataatacta
     1561 ttgtagtgtg ggatgttagt tactaaagga tgacgcttat atatatgact gaatagaata
     1621 agcaa
//
LOCUS       DAZUKA010000276         1625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000276 DAZUKA010000000
VERSION     DAZUKA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.282"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACG8PN_002998"
     CDS             <1..1604
                     /locus_tag="ACG8PN_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS4863071.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          595 a          267 c          331 g          432 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaatt
//
LOCUS       DAZUKA010000277         1614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000277 DAZUKA010000000
VERSION     DAZUKA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.283"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..1465
                     /locus_tag="ACG8PN_002999"
     CDS             278..1465
                     /locus_tag="ACG8PN_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4863072.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          549 a          267 c          333 g          465 t
ORIGIN      
        1 actaaactgt cgaaagctaa cacaataaaa aaagaagctt atgaattaga acaaaaaaat
       61 gatgaaaaac taaataaatt acatgataga gaaagattta tattaagtaa ggaagaaaca
      121 ctatcagatt taaataaaca aattactatg ttaaaaaatg atattaattg attacgtcca
      181 tataattgtg taaaagtgaa gcatcaaaaa agccattgat ctcagttagg agtacaatgt
      241 tttttgcaga aaacatactc gaataggact gatatcaatg actcaagtac attttacttt
      301 tgaaagcgaa gaaattcaag caattataaa cgaaagcggt gcgaatgata ccgcaaaaac
      361 acttatgact atcatgttta atcagctgat ggaagaacaa cgaaatcaat atattcaggc
      421 aaatgcctat gaacgttcag aagaacggca atcacaaaga aatggttact acgatcgttc
      481 gtttacaact cgaattggta cgttagaact tcatgttcca cgaaccagag acggtaagtt
      541 tagtcctact atctttgaaa gatatcagcg aagcgaaaaa gctttgatcg ctgccatgat
      601 tgaaatggtc atttctggtg tctccactcg caaagtaact aaaacagttg aactgctaac
      661 ggatggtgcg actgtttcta aatcattcgt ttcaaatctg atgaaacagt tggatccatt
      721 tgtttttgag tggagaaacc ggagcctaga aggctcggaa tatccatttt tcatgtgtga
      781 tgccctgtat atgaaagtaa gggaaaatca tcgtattgtc tcaaaaggtg tatatattgg
      841 tatcggcatt gattctgatg gacgacgtac gattcttggt tttgatgttc aagacggcga
      901 atcggaagat aattgggata ccgtttttca atcgttcgtt caacgaggtt tgttcggtgt
      961 aaaactggtc atttctgatg cacacaaagg gttagttaag gctgttcgca agaacttttt
     1021 aggtgcgagc tggcaaagat gccaagccca ctttttgaga aatatttttg ataagctgcc
     1081 aaagaaagtt tcttctgatg taaaagacga gttgaagagt atctttaaag cctctgaact
     1141 tgaattgaca cgtgaaagaa aagagcactt cttggaaaaa tatggttgtg attcaaaatt
     1201 aagtgctgct tgcgacattt tagaaaacgg ctttgaagat gctattcaaa ttttgtcttt
     1261 tcctgaaaat atccgccgaa ggattcgtac aaccaatgtg ttggaacggt tgaacgaaga
     1321 gattcgccgg agagaacgag tgattcggat ttttccaaat atcaattcca tcactcggat
     1381 aattggaaca cttttgatgg aaaaagacac tgagtggctg gcttctccac gaaaatattt
     1441 agaatttaat tcgaataaca tttagcacca tgaacctgtg cgagtctctt ctttctgagg
     1501 agagacatca agagtcgctg cgctactgct cctgacctct ctcctcagaa agaaaggcat
     1561 ggcagttagg ttcacaacgc aaaacattgt gcttttgatg aggttgaact ttta
//
LOCUS       DAZUKA010000278         1612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000278 DAZUKA010000000
VERSION     DAZUKA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.284"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1612
                     /locus_tag="ACG8PN_003000"
     CDS             <1..>1612
                     /locus_tag="ACG8PN_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4863073.1"
                     /translation="KDIDTFLNTVTIYAQSRTNRLGRRVVTLVEVDSKTGQEAISIIE
                     TKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLLQYWGGEIKREPFKLSLLK
                     RRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQSEVGTTNRIYGSPVDSAYI
                     NNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGCDKPKVSITVEFDKLTDSE
                     EAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSLSEKTLKLEAGDIQVAFYQ
                     QQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPD
                     LEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKT
                     GDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTP
                     IEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQ
                     GGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          592 a          203 c          326 g          491 t
ORIGIN      
        1 ataaagatat agatactttt ttaaatacag taactattta tgctcagagt cgtacaaatc
       61 gacttggaag acgggtagtc actcttgtgg aagtggactc taaaactggt caagaagcga
      121 tatcaattat tgaaactaaa atggataaaa aatctgacat acgactttat tctgatatta
      181 cggctgtttc tagcacaatc tttgaagcga gaaatgtttt aaattgtatt gctggtgaac
      241 aaggatcatt gcttcaatat tggggcggag aaattaaacg tgaaccattt aagctttctt
      301 tgttaaagcg aagaggtcgc aataatgttg gaacgattcg gtatggaaaa gatatgtctg
      361 gtttgaaggt caaattagat tggacaggta taaaaacaag aattattcca tatgctgatc
      421 ctcaaagtga agtaggtacg actaatcgaa tttatggttc gccagtagat agtgcctata
      481 ttaataacta tcctgatgtc tatacagaac atgttcagtt tacagaagaa caaggagtaa
      541 aagacattaa gagcttaaat aaaatagcta aaaattactt taaaacgatc aatcctggct
      601 gtgataagcc taaagtttct attacggttg aatttgataa gttgactgat agtgaagaag
      661 cgaaagaatt tgcgaagatt agaaattatg gtctgtttga tacatttaaa atatatcata
      721 aaaaatataa tatttatctt gaatcaaagg ttagtggact acaatacgat tctttatctg
      781 aaaaaacttt gaaattagaa gcaggagaca ttcaagttgc tttttatcag caacaagctg
      841 taactattca ggataaatta aaagattatg caacgaataa ttatatgagt gattttaatg
      901 attatgtttc ttcaatgatt acaggtcaag gcagtgcagg aggatatgta gttttatggc
      961 caaaagaaaa accttccaac atctttatta tggataatcc agatttagaa aaggcaagag
     1021 aagtactaag aatgaataaa aatgggattg ctttttctaa gaaaggatgg cgggggcctt
     1081 ttaattcagc ttggacatta gatagtatat ttaatgccaa ttttatacaa actggattaa
     1141 tcaaagcaga tatctttcaa aattctttta acaagactgg agatgtattg aaattagtta
     1201 atggactact ccaaatttgg aacaacaaaa agaaaataat ggagttaact aaaaaaggaa
     1261 tggaattttg gaattctaag ggttcaattg gaacgattgg aacaactgat tctgttggta
     1321 atccttttcc tggggcttct actccaaccc ctattgaaga taattcttta gttattcgta
     1381 caaatggaga cggcaaatat attttgattt ctcctaaagt aggaaaagga ttagttttat
     1441 taggaaatgg taaagcaatt tattttggag acttagatgt acaaggaaaa cttacagtta
     1501 aaggaaaaga aataattcct ggacaaggtg gtggtccatc gggaggtgga gaatctactg
     1561 gtgggtatcc tagtgaatta aaaacagatg cagaaaagag agcttggaga at
//
LOCUS       DAZUKA010000279         1604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000279 DAZUKA010000000
VERSION     DAZUKA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.285"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACG8PN_003001"
     CDS             <1..259
                     /locus_tag="ACG8PN_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863074.1"
                     /translation="NNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDV
                     DRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(621..860)
                     /locus_tag="ACG8PN_003002"
     CDS             complement(621..860)
                     /locus_tag="ACG8PN_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863075.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1146..1460
                     /locus_tag="ACG8PN_003003"
     CDS             1146..1460
                     /locus_tag="ACG8PN_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863076.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          551 a          219 c          281 g          553 t
ORIGIN      
        1 gaataacgaa acaatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca
       61 aaaagtgatt ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga
      121 atatcatgac gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata
      181 tactcacgtt tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga
      241 acatacttta gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct
      301 tttgaaggca ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg
      361 ttcgcctaga gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat
      421 actgccagaa ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga
      481 tccaatcatt tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc
      541 gtaaagcatt tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt
      601 tgctgaagaa gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac
      661 agtttcttta ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga
      721 gaatttttct catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa
      781 ggtatgttct ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg
      841 ataaacagaa ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta
      901 tgctcagtat taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc
      961 ttagtaatac atatatggag agggtgatat cccagttcaa accagataca ttggttacca
     1021 tctgtattaa cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac
     1081 gtaggtatga tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat
     1141 agcttttgga tttttacagt aattttatct taataatagc aatattatta cttctaaata
     1201 tttggttttt tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt
     1261 cttctgaaaa aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca
     1321 gtttactttc ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta
     1381 tatctattat tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag
     1441 agaagtcaaa aaataattaa agcaattatg ccttttagta caggaattat tacttcttac
     1501 aaatgtatag cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa
     1561 caattatttt atgttaggat gaattaattt ctctagagtt tata
//
LOCUS       DAZUKA010000280         1593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000280 DAZUKA010000000
VERSION     DAZUKA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.286"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8PN_003004"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACG8PN_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          466 a          313 c          251 g          563 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt att
//
LOCUS       DAZUKA010000281         1578 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000281 DAZUKA010000000
VERSION     DAZUKA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.287"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            759..>1578
                     /locus_tag="ACG8PN_003005"
     CDS             759..>1578
                     /locus_tag="ACG8PN_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4863077.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          565 a          217 c          242 g          554 t
ORIGIN      
        1 cttttagata atttttaggt ctatttaact catcagacat tctatgctca tttttctcca
       61 aatttgtttc aactctttcg tacttaattt tgcgtatttg aaaattcttc ttttagagta
      121 cttttattac gatactagta tgatatatat ctatagcaaa cagttaaaat cttcctaaaa
      181 taagcgaata gcattcctaa aacctataaa atttttagat gaagcgacaa gtggacaatt
      241 aataaactac ttgaacatga aattatggat cacctttcaa atttagaaaa tggaattctt
      301 atacttattg cgcaacattt attgattgca aaattatgtg acaaaaaaaa ctagtagaag
      361 aaagaataat ggcttgataa taaagtacta cctattaaag gtagtacttt atttaattta
      421 gtataaatta acatactact atattttagg tttcatatta tataaattaa taatatacta
      481 ccatttgttg gtagtataat ttaaaggtaa taagttattc ttttattact atgtttgggt
      541 tttatagcaa agaaagttta ataagagttt ggacctagca aacaatatta atgaggtcat
      601 taaatcttat tatctttagt gatataacga aatatctaca cagacaaaat gatagcgtaa
      661 aatattttgt gtaaatagat atttagggta gaaaaaaaga aagtccattc tgtaaaatta
      721 aagttaccac aaccgaaatt ttataggagg actttctcat gcccaatttt actacagaaa
      781 ttatggaaac actaatcaat aaaggtgatt tagatgaatt atttcgatgt catttagaat
      841 tagctgttaa ttcattattg caggctgaat taacagcttt tcttgattat gaaaaatatg
      901 atcgtgctgg gttcaattca ggtaattccc gtaatgggaa ttattcacgt tcattcagaa
      961 cagagtatgg agaattaaat ttggtaattc ctagagatag aaatggtaag ttttctcaac
     1021 aaactttacc tgcttataaa agaaccaatg actctttaga aaccactatt attcagctat
     1081 ttcaaaaagg aattactatg tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc
     1141 actatacgcc acaaaccatt tcaaatatga ctaaaatagt ttctgaagat attattgcct
     1201 ttaaagaaag gtccttagaa tccagatact ctgtcatttt tatggatgct actcacattc
     1261 ctttaaaaag acaaactgtt tcaaaagaag cagtttatat tgtaattggc attcgattag
     1321 atggaaccaa agaggttctt gggtttagtc ttgctccaac tgaatctgct tatgtttgga
     1381 aagagattct tcaagaccta aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg
     1441 atggtttaag tggcattaac gatagtattc atagtgttta tccaaatgct caatttcaac
     1501 aatgttgtgt ccatatatct agaaatatcg ctcataaggt tcgtgttagt gatcgacaag
     1561 aaatctgtag cgatttca
//
LOCUS       DAZUKA010000282         1555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000282 DAZUKA010000000
VERSION     DAZUKA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.288"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1452)
                     /locus_tag="ACG8PN_003006"
                     /pseudo
     CDS             complement(17..1452)
                     /locus_tag="ACG8PN_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          516 a          254 c          261 g          524 t
ORIGIN      
        1 attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta tgaattgttt
       61 gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg tataaagaac
      121 tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt tctctaagtt
      181 gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct aactgttgtc
      241 gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt ggagatttcc
      301 gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa ttctcattcc
      361 aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga acttctaagt
      421 acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat atctgaatat
      481 cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc tgattttctt
      541 cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa gggttatctg
      601 ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag ttatcgcata
      661 gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg tcatgacata
      721 aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga gtatgtcggg
      781 caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc tgcaaaccga
      841 tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt gaaaaaaatg
      901 tttttctaca taatcaatca attgttgacc gctttgaata attgatttat ttagctggat
      961 caactctgtc cgatttgtct catagattat cgttagtaac aaacttcgat aatccttttc
     1021 aggaatttca aaatggccat cagcatttaa gtactgcatt tgtttaaaaa aattaatcaa
     1081 ctcatttcgt aagttgtaaa ctttgcttaa cgaaatgaag gtagtatcag ctatattact
     1141 ccaattgtgt tcccctgata aataatgtcc tagtacattg aaaaagtcag agcgctgata
     1201 gatgagttga gtgagtgcca acctcgaaaa cgaagagtcg aatgcgactg taaagctact
     1261 tctagatttc tccactgaaa taatttgctg atggagtatg tcggtaatgc attccaagtc
     1321 gttagtgaca gtgagtgggc aaacgttcaa ttgatctgct atttcgcgaa tctgaatggt
     1381 ccctttttcg tgtaattgct cacaaatgta aacttgacga aaaatacttt tctccagata
     1441 ggtttccaac atagtactcc tcctagtcat tttatacata actattataa ctctaccaat
     1501 cggaatagtc ctgtaatata ttgaatcaaa aatataaatt ataaaataat tagat
//
LOCUS       DAZUKA010000283         1553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000283 DAZUKA010000000
VERSION     DAZUKA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.289"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1535
                     /locus_tag="ACG8PN_003007"
     CDS             57..1535
                     /locus_tag="ACG8PN_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863078.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          558 a          216 c          285 g          494 t
ORIGIN      
        1 gaactttcat ctgcatatca agtaattggt atgaggcatt ttttgaggtg taggatatga
       61 agttggatta ccttatagaa gaggaagata gagaaaaact gctgctagct agatatttag
      121 aatttgagaa aaagaattat gtagtgctgc ctaagttata tgaaacatta ggtttgtcta
      181 aatttaaaac aagaaattat atcatagaat taaataaaga atttactgaa ttcaaaacaa
      241 agccagagat ccaaatttca gaagaagcag agatccatgt cgaacgtctg aatttgacgg
      301 atatcaaaaa tttaagaaga atctactttc aacgatcaaa aattgctcga ctgttaacgg
      361 agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt ttaagcatat
      421 caagagccta tgtaaaaaga agagaattag tctcttttct tgaacagcat caaataaagt
      481 taaagaaaaa catattagtt ggggaagaaa taaacatacg gaacgtatta tttggggtat
      541 atttcggggt ctttaatggt ttttctatgc catttagaga aagcgtcatg aatgaagcaa
      601 agagtatcgt gcaatatttt gaatatctgt tcaatttaaa actgtctgca acaaaacgaa
      661 tcaaattcaa tttgctaacc actattagtt tgttgagggc ctatgggaat caaccaatcg
      721 gaaaacgttt cttttcagat gaatacttta agagctcaga aggaatgaag atcatcaagg
      781 atttatcaca tatttctttt cttcatatcc ctaaagagca gatcattgat gagatttctt
      841 atatcatgtt gtttttgatc acggaagaag aatttgaatt agatggcgca tttggacttg
      901 cggaagctca ttttcatgat atccacatga ttagtcaaaa aatgtcagtg gaattgattg
      961 atcaattacc attttcgatt gaaatctctg aagaagaaag actatatata cgaaaagaac
     1021 ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat attcgacttt gagatgacta
     1081 gctttacgtc taaaaagcag ctacagtttt tcttcgagac gtatccaata ttcagtacag
     1141 caatccaaaa ggtcatacaa gagaataaac gctatttctt gggaaaagat gaacgtttca
     1201 tgattcgctt attttatgat tatctctttt tattggtcag tatttgtccg gtatcatatt
     1261 ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg atctgcttat accgattaca
     1321 ttatttctca agtgaaagga tttaaaaatt acaatattgt gattgagagt cgaatcactt
     1381 ctcgtacgaa tatttattta tctgattgtt tacttgaaaa atttaataaa gaacagataa
     1441 tttggaaaaa tccaccgact ccagatgact gggaatattt tggaaatacc atcatcagag
     1501 aaaaaaatag acatttaact gaaaaaatca gctagtattt atggatggca gct
//
LOCUS       DAZUKA010000284         1504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000284 DAZUKA010000000
VERSION     DAZUKA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.290"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG8PN_003008"
     CDS             complement(37..993)
                     /locus_tag="ACG8PN_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863079.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1118..>1504)
                     /locus_tag="ACG8PN_003009"
     CDS             complement(1118..>1504)
                     /locus_tag="ACG8PN_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4863080.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          470 a          245 c          241 g          548 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aacttgggag cgtcaataat
     1081 tttgtgtaaa taaattgtcc tcctgcaaaa taattagtta ctcagtaaac attgaaacta
     1141 atgtatcggt tacctgttga aaacctttat ggcttctgtt tagaaatttt tgattgtatg
     1201 tatcaaaaat gctgactaga aagcgttcta gtgattcttc attttgaaac tgctcttttc
     1261 tacggctgta tcttttaatt tgcttattga aagactcgat tagattggtt gagtaaatgg
     1321 ttctacgaat gctaggtgga aaatcataaa aagttaataa gtcttggttt tctatgagtg
     1381 actgcgtcac tttaggatag tttttcttcc atttctcaat catgccggat aagaaggtat
     1441 tcgcttcttc ttttgagtta gcttgataaa cagccttaaa gtcatcacag atttcttttc
     1501 ggtc
//
LOCUS       DAZUKA010000285         1492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000285 DAZUKA010000000
VERSION     DAZUKA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.291"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1171
                     /locus_tag="ACG8PN_003010"
     CDS             <1..1171
                     /locus_tag="ACG8PN_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFS4863081.1"
                     /translation="FSENALNKKVNDFLLSNGLNEEEYPTIFEIDYSYALKEEEYQLL
                     AIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHYRQILYSNANKSGDE
                     ILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPELSVSITDEIDIDGLYDN
                     NVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDILIFVTKENDVDLNGEK
                     SGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRNSANDDAGETAEQIAYA
                     ELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGNMYFEVKSSITEFEM
                     SINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            1306..1434
                     /locus_tag="ACG8PN_003011"
     CDS             1306..1434
                     /locus_tag="ACG8PN_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4863082.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          576 a          199 c          282 g          435 t
ORIGIN      
        1 gtttagcgaa aatgcgttaa ataaaaaagt taatgacttt ttgttatcaa atggactaaa
       61 tgaagaggaa tatcctacca tttttgaaat tgactattca tatgcattaa aagaagaaga
      121 gtatcaattg ttggcgatag ctctagaaag catttcaaaa gatattgagg atttgaatgg
      181 attgtcagaa ttgattcatt tggatattcg tgaattttgg aaactgaaat taagatctta
      241 tctagataag aaagcggaac attaccgcca gattttatat agcaatgcaa ataaaagcgg
      301 tgatgaaata ctcaaagaaa cattcctaga aaaaattgat agatacaaag attatcaatt
      361 tgagatttct gaaattccaa attcagttca agtcgacatt gagaaagaaa gtaagaagag
      421 atttccagag ctaagtgtgt caattactga tgaaattgac attgatggcc tgtatgataa
      481 taatgtcgaa gagttagtca cggttgcagc tattacaata gatgattttg attattatat
      541 tagaggtcat caaaaagttt cttccttact gtattttggt attccgtccc gtttaagaga
      601 agatatactg atattcgtta ctaaagaaaa tgatgttgat ttgaacggag aaaaatctgg
      661 tatctctatt gaaggaacat caactgttca aaccattctt gaaccatcga atagtgttag
      721 taggaaaggt aagaacacac gaaatggaga aagaagtaaa cgagattatg ataatagaaa
      781 ctccgcaaat gatgatgctg gtgaaacagc agaacaaatt gcttacgctg aattgactca
      841 gaatcaaaaa cttaatgtaa tttggaatac gaaatattct agtaaatctt cagatcgtaa
      901 taagcagcca ccaaacggaa ttgtttgtga tatgtgggtt catgacccag agaatgggaa
      961 tatgtacttt gaggtaaaat catcaattac agaatttgaa atgtcgatta atgaatataa
     1021 ttctatgtta gataaccctg actcttacga ggtcgtttta gtcaatcgtg ataccaaaga
     1081 gataagtcgt cataaatttg atgaacttga tggattgaaa caggcaagta gctatctttt
     1141 caaatttaag caaaagagaa gtgacggttg agtgatttgt ttccacaaac ctattcaatc
     1201 tatcaactca aaaatcataa acaaccatta ttatagtagg aagatacaga gtgtactctc
     1261 aacgcatatc gaattagtcg cttcattcgt taaaggagaa taaaaatgga ccaaaaagaa
     1321 aaaatgaaag aattgatcaa aaagaaacaa ggtggaggcc gatccaaaca aatggaaaca
     1381 cctaagaatg atcgtaaaaa catgagaaaa ggccctaaaa tctttaataa atagacgtgc
     1441 taaagaaaga ctaagaagtg ttaacttttg ttattgtttc ttagtctttt ta
//
LOCUS       DAZUKA010000286         1485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000286 DAZUKA010000000
VERSION     DAZUKA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.292"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8PN_003012"
     CDS             <1..969
                     /locus_tag="ACG8PN_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4863083.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACG8PN_003013"
     tRNA            1159..1232
                     /locus_tag="ACG8PN_003013"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          490 a          254 c          294 g          447 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt ttgaagagta ttatacaagc taaagctttt cttcattgcc actcaaatga
     1321 gtggcttttt tatgtatcct tttatggatt aatgaaagga tgtttcacat agttatattt
     1381 ctgtatattt gaaaagtttt actttgattt ttaaatagaa agacatttgg gttatattgt
     1441 gagataataa taaagaagag tttaaagcgt tccccaaaaa ccact
//
LOCUS       DAZUKA010000287         1467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000287 DAZUKA010000000
VERSION     DAZUKA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.293"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..361
                     /locus_tag="ACG8PN_003014"
     CDS             <1..361
                     /locus_tag="ACG8PN_003014"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4863084.1"
                     /translation="SSLIEDWAIFMAKEGKEMFQNKGQRYVTKGVMDSLPVELQALCW
                     NLIDQNVQKQFHLDYLQIFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKI
                     WVIDSGEYQTMLLPEEY"
     gene            388..687
                     /locus_tag="ACG8PN_003015"
     CDS             388..687
                     /locus_tag="ACG8PN_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS4863085.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            861..1412
                     /locus_tag="ACG8PN_003016"
     CDS             861..1412
                     /locus_tag="ACG8PN_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS4863086.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
BASE COUNT          531 a          210 c          302 g          424 t
ORIGIN      
        1 atcttcttta atagaagatt gggctatttt tatggcaaag gagggaaaag aaatgtttca
       61 aaataaagga caacgttatg tcacaaaggg agtaatggac agtttgccgg tggaattaca
      121 agctttatgt tggaatctaa tcgatcaaaa cgttcaaaag caatttcatt tagactatct
      181 tcaaatcttt gaattttcta cggaaaaagg aaatcaaaag ttggttcatc gtcaagaaga
      241 accagaagca agaaaagagt atctgatttc acctaagctt cgtttgaaat cagtgtctca
      301 aaaaatatgg gtcattgatt cgggtgagta tcaaacgatg ttattaccag aagaatactg
      361 atatctaaaa gaaaggaacg aaaggacttg ttagaaacgg ctatcgaagt actggaaaag
      421 tgtgcacagc tagtcactac tcctgaagag tggggctatg aatccgttac gatggaaaaa
      481 gaggaaattg aaatgggtat gcttcctgaa gatgttactc taccacgttt agtgatgaca
      541 catctttata tttattgcgc accagaagat ggaaaagact atgtagttta tttcattaca
      601 gatattacca gccagcgaga atttgttcgt gggttactgg tagaaggacg attggtttgg
      661 agtcagattg gaggaacgaa tgaataagat cgattatttg agaatgattg cgaaatgttt
      721 agaagatatc cttacagcga ttcatcaaat tctgaagaaa aaaggagaaa gaatatgaaa
      781 tattttcaac tagtattgac acttataacg gcaatggtga ttgaattgct tgaacaaaaa
      841 ttagcatagg agaaaaagag atgattgaaa aagaattagt gaccacacaa acagaagttt
      901 ggcgagatcc tcatcatacc catcgctatg ttttcaaacg acaatgggcg atcaaaggaa
      961 aagaagcgga agataaatta gcggtagtca ttactattcg tccaacagat acagaaccat
     1021 ttacgaatga tttgagtatg ttattaatcg aaaagaatat ccgacaatta ggatttactg
     1081 gttttattgc tgtgaatctt ttttcgtata caaaagcgaa aagtcctcgg gtatttcctc
     1141 gtggaaatga tgaacaatct ttggaaatat taacaactgt tttcacagaa aagaaaatca
     1201 acaccattat ttttgcttgt ggttcgatta cagcgacgag tcaagtggct tatgaacaag
     1261 cgaagaccat ttacgatcaa ttgagtgcga aacaaaaaaa gatgaccaaa gtattgataa
     1321 atgcagaagg taagttatcc cacccactaa gtattcatgt tcgtaatgaa tggcaattgg
     1381 cagatagtca attattattt caagaaaaat aacattttat tttcttatgt tacttataag
     1441 tagttttaca tgctttttga ctttaaa
//
LOCUS       DAZUKA010000288         1457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000288 DAZUKA010000000
VERSION     DAZUKA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.294"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(429..1043)
                     /locus_tag="ACG8PN_003017"
     CDS             complement(429..1043)
                     /locus_tag="ACG8PN_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4863087.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          474 a          278 c          242 g          463 t
ORIGIN      
        1 ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat
       61 tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt
      121 tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt
      181 ccactacttt ttatttttct gacactacat attcttataa ctcatttctc ataactgtga
      241 aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat
      301 cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct
      361 ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat
      421 gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact
      481 tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt
      541 ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat
      601 tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca
      661 gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc
      721 gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga
      781 tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca
      841 atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt
      901 agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa
      961 gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa
     1021 tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga catgaataga
     1081 aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg
     1141 gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata
     1201 gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct
     1261 agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca
     1321 gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc
     1381 ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca
     1441 agcaagaaaa tacaaaa
//
LOCUS       DAZUKA010000289         1452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000289 DAZUKA010000000
VERSION     DAZUKA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.295"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..140)
                     /locus_tag="ACG8PN_003018"
     tRNA            complement(68..140)
                     /locus_tag="ACG8PN_003018"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Gln,seq:ctg)
     gene            complement(186..614)
                     /locus_tag="ACG8PN_003019"
     CDS             complement(186..614)
                     /locus_tag="ACG8PN_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863088.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(674..1432)
                     /locus_tag="ACG8PN_003020"
     CDS             complement(674..1432)
                     /locus_tag="ACG8PN_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4863089.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          415 a          281 c          274 g          482 t
ORIGIN      
        1 acaataatcc ccctgcaaaa agattgttct tttacagggg gttcattttt tcagcaagca
       61 catcaaattg ggatggcagg gttcgaacct acaattgctt gattcagagt caagtgcctt
      121 accagtttgg ctacatccca tttattcaac aaaataatca tagcactttc ctaaaaaaac
      181 gcaagttatt tttcttgatc ggatagttgt tgctttactc gtaaaaggcg gcgtactagt
      241 ttcttttcta atccagcagg aagggcaatc agttctgcta ctgacaagac ggtgtccaat
      301 aactgatatt ctttgacagg ttcattcata atatgataga tgtttgctaa acgttcaata
      361 tattcaaagc gagtatactc tgttcgagca attagaaatt tcaataaatc ggtggctttc
      421 tcgtactctt gatctgcgta ataaacaaga gattgacgat acaatttgat ttccagttca
      481 ttgagggtag caggtctgcg acgctcgttc cactcattaa tagagacttt accttgaaca
      541 tttcccttgc agtcacggac aaaaataatc ggaatttcat ttggatccat ttttttatgt
      601 gtgaattttt ccattatcta cgctcccttc aaaggtttgg tatcttgagt ataagggatt
      661 tttgtttgaa ttactagctt ttctccaaag ttagcatctg tccacccatt gcagtgaaaa
      721 tcgccgaaaa atcgttgatc ggtactgctc gattttttac accataggga tcgttgacat
      781 aaatattttc tttgtcatat ccagtgacta cacaagcatg aacaagagga gtgactctta
      841 cttctccatt atttgtggtc caagtcataa aatcagtttc atctgggact tcaaaattga
      901 tagtcacaga agtccagaca ggatttcctt tagatagtgc ttttttgata tcatcgagtt
      961 gagcatcggt ttttggaaaa gtgtgataat ccgtttggac gatttgctga gcaagggagg
     1021 caactggggg aacatagacg accatagccc agtctccgcc actgatatca ccaacaaatc
     1081 cttcattagg atcgccatga gtcccatctg cattgaaaac agggacgtaa tccaattgct
     1141 cagctaactg gtttttagtt gtttcataac cataaaaaga taaaagcata gaaagcgaag
     1201 tgacttcaca gccattttct aaagaaggag aatcaaactg gctctctaat ggaacatcta
     1261 gtaaaacttc tgctttctct tttgtctttt tcgttttttg tgttgaactt ttttgtgcag
     1321 tgttcattgt atgttctgga gaatggatat gtaggtaata acttgtaccg cttaataaaa
     1381 gagcagctgt gccccctaga aacaatagtt ttttgaacgt ttttttcttc atttgaaatc
     1441 tcccttttca gc
//
LOCUS       DAZUKA010000290         1444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000290 DAZUKA010000000
VERSION     DAZUKA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.296"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..365)
                     /locus_tag="ACG8PN_003021"
     CDS             complement(<1..365)
                     /locus_tag="ACG8PN_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4863090.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSA"
     gene            complement(347..1321)
                     /locus_tag="ACG8PN_003022"
     CDS             complement(347..1321)
                     /locus_tag="ACG8PN_003022"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4863091.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          469 a          269 c          188 g          518 t
ORIGIN      
        1 gctgactggg ccattaataa ctccagtaaa agaggtaaat taatcccagc aataatttga
       61 attctattgt tgttcaactg tttaatagaa acattattag gtgttcctcc aattaaatcc
      121 gttaaaataa ttagaggttg caaatgatgc tgactaatta tttctttcat actctcctca
      181 aaatcatctg gactagtatt ttctttaaat ggaacagcac taatacctgt ggcgtcattc
      241 attaaaatta attttgttgc ctttaaataa ccctcacaaa gggtagaatg tgttgtaatc
      301 acaatgtgaa ataattgatt tgccatcttt tgcaacctcc tattaattaa tatttcattt
      361 gccacatata gcgtctaaca tctaaggaat ggcctctttc aaaagcaatc gcttctacca
      421 tttggcgaat aacaacgcca ctaagtacag ctgcaatata ttctttcgct acactatcta
      481 tagattctaa gtcaaaatca gcttggtcta aaaccattaa tttattactg aattttttag
      541 tgaagtcttc tacccgttga tctaaaaatc ttgtatctcc aatacttttc actaagatga
      601 atggtacatc ataatcagtg atttcaaaag gaccatggaa aaattcccca gaatgaatag
      661 cattagaatt aatccattgc atttccatga accagcaagc tgctgtggag taaatttctc
      721 cgtaattgat accgctacca attgtgtaaa taattttatc tcgtttttgg tttttacccc
      781 attctttagc agttatttca aatttgctct tttcagcaat aacaagatca tttaacttat
      841 caaattcatt caagcaagtt tcccattttt tagagccatc taaaacaagt aaaatattaa
      901 ataataaacc ataaagaatt cctttattta aatcactagc atcaacatct ttaccccaat
      961 catagtgaac aggatattca gctgcttccc atagaggaga tccttcttca ttagataaac
     1021 aaatagtcat tgctccaaat tctcttgctt tttttgttgc ttctacagtt tctggtgttg
     1081 tgccagaatg ggaacaagtg ataactaaag aattttcaga taatcctttt ggtatagcat
     1141 agttaaattc gttagcagta taaacatgac aaggtacagt taattcttta tcaaatagat
     1201 attgaccagc cattaaaacg gcttgtgaac ccccacatgc tgtaaaatat acatgatcaa
     1261 ttgtcttttc ctttaacgct gccattgctg tttcaatctg ttctttgtgt agttgtctca
     1321 tttctttatc cctcactcta ataagtatta taaagtcata taactttaca atacctatta
     1381 tctatgaaat tgctttattt tgcaatacta tttttcaaaa aaattttttt ataacaattt
     1441 tata
//
LOCUS       DAZUKA010000291         1402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000291 DAZUKA010000000
VERSION     DAZUKA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.297"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1184
                     /locus_tag="ACG8PN_003023"
     CDS             <1..1184
                     /locus_tag="ACG8PN_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4863092.1"
                     /translation="CLTIMKSSNEIEKALYESSNSSISITKKDGKYFNVNQFKDIEKL
                     KEIEEKIIQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSG
                     VFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIG
                     IFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLA
                     LNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILIL
                     WLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFIN
                     SEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKI
                     S"
     gene            1194..>1402
                     /locus_tag="ACG8PN_003024"
     CDS             1194..>1402
                     /locus_tag="ACG8PN_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4863093.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDI"
BASE COUNT          588 a          126 c          228 g          460 t
ORIGIN      
        1 cttgtttaac aataatgaaa tcaagcaatg aaatagaaaa ggctttatat gaaagttcta
       61 attcttcaat atcaataaca aaaaaagatg gtaaatattt taatgttaat caatttaaag
      121 atattgaaaa attaaaagaa attgaagaaa aaataattca atatgatgga ttagcaaaac
      181 taaaagatgc taaagtagtt agcggagaac aaagaataaa tagagaagat ttatctgacg
      241 aatttaagaa tgttgtttca ctcgaagcta caaataatac taaaagaaat attttattta
      301 gtagtggagt atttacaatt aaagaaggta aaaatataga agaaaatgat aagaattcaa
      361 ttattgttca tgaagaattt gctaaacaaa acaatctaaa attaggtgat gaagttgatc
      421 ttgaattact agatattgaa aaaagtggaa aaataaaaag tcataaattt aaaattatag
      481 ggatcttttc tggtaaaaaa caggaaacat atacaggatt atcttctgat tttagtgaaa
      541 atatggtgtt tgtagattat tcaacaagcc aagagatatt aaataattca gaaaataatg
      601 aaattgcaaa taaaatttta atgtattctg gtagtgcaga atctacagac ttagccttaa
      661 acaaattgaa agagcttaaa attgatgagt caaagtattt tgttgaaaaa gattctaatg
      721 cgtttgaaga gtctttagag tcagtgagtg gaataaaata tataataaaa gtaatgactt
      781 attctattat gttaggtggg atggttgttc tttcattaat cttgattcta tggttaaggg
      841 aaagaattta tgaaataggt atattcttat ctattggaac atctaagata caaattataa
      901 tgcaatttat attcgagtta atattcatat cgataccaag tataatatct tccttatttt
      961 taggaaatgt attactaaaa gtaattgtag atggattcat taactcagag gattcaatga
     1021 tttccggtgg aagtttaata aataatagta gttttatgtc aaatataaca acacttggac
     1081 aaagttattt aatattaata agtattattg ttttatcagt tgtatttgct tcttcattaa
     1141 tgttaatcaa gaagcctaaa gaaatcttat caaaaataag ttaggagaaa ataatggata
     1201 tattagaaat aaagaatgta gcatatagtt atgcaaattc taaagaaaaa gttttgtcag
     1261 gagtaaatca aaaatttgaa cttgggaagt tttatgcgat agtaggaaag tcaggaacag
     1321 gaaaatctac acttctttcc ttacttgctg gacttgataa acctcaaaca ggaaaaatat
     1381 tgtttaagaa tgaagatata ca
//
LOCUS       DAZUKA010000292         1390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000292 DAZUKA010000000
VERSION     DAZUKA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.298"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..966)
                     /locus_tag="ACG8PN_003025"
     CDS             complement(7..966)
                     /locus_tag="ACG8PN_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4863094.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1110..>1390
                     /locus_tag="ACG8PN_003026"
     CDS             1110..>1390
                     /locus_tag="ACG8PN_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4863095.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTR"
BASE COUNT          417 a          236 c          269 g          468 t
ORIGIN      
        1 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
       61 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      121 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat ctcgtctaag
      181 caagccgttt gagtgctcat ttagccctct ttgcgaaggt gttcctggat ctgcgaaaaa
      241 gatggcaata tcgttcgagt tacttataga tcgccagttg gaaaattctt ttccacaatc
      301 aaatgtcatg gatttaaaca agtgtttagg catggatttg aaccaatcat ttaacctgtt
      361 ttcgatatct tcggctcgtc tgcctgtcgg tttcaaagta ataataacct tggataaaca
      421 ctcaaccaat gtaataacag cgctcttgtg tttaactccc acaatggtgt cgacttcgag
      481 atgaccaaat tctttagtca ggtgtggata gagactgttc cgttcatgaa tcgttctgcg
      541 aaaggcctgt tttccacgct tttcttgatg attattcggt ttacgtttcc ccttcatagg
      601 taacgactgt gtgtcaaaaa ggcctttttt aaacatccga tagagtgtac gaacagaaca
      661 atcaattgga aactcgccac ggccgataat cacatcgggt gaccatcctt gagctacctt
      721 ttcttgaaca tatgttgatt gttcatcaga taaaacggtt gaacgtcgtc cacaacgaga
      781 tttattttgt ttgtattgac ggtaataatc tagcactttt tttccttgtt tgagaaacga
      841 ataaacatta taaatggttt gtctagagcg atttaaactt tgtgcgacaa gagcgacagg
      901 tttctcgata ttgaaataag actctatgat tactagttcg tctgttgtaa gatgggtgta
      961 agtcatttgt actcactcct tattaattct ttggtcggaa ctattttgag tgtagcacaa
     1021 atgatctttt tagttgtcta gcttaatttt acaatcgacg ttttaataaa ttgtgatata
     1081 atgaagtata actttttagg gagtgtacta tgaattttca agataataga ccattatatt
     1141 tacaagtaga agcttctata cgtaacgata ttcgtgaaaa aaaatatctt ccaggagagc
     1201 aattacctac agaagaagaa ttatgtaaaa tttatggagt aagtaagatt accattcgaa
     1261 aagcttttaa acttttagca gaaagtggaa cagttgaacg attaaggggg aaagggacct
     1321 ttgtaactca aaaaaaagaa agtttaaaat tagctggagt tcatggattt agcgattctc
     1381 tttctacccg
//
LOCUS       DAZUKA010000293         1374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000293 DAZUKA010000000
VERSION     DAZUKA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.300"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACG8PN_003027"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACG8PN_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4863096.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAZUKA010000294         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000294 DAZUKA010000000
VERSION     DAZUKA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.301"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8PN_003028"
     CDS             82..>1364
                     /locus_tag="ACG8PN_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4863097.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZUKA010000295         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000295 DAZUKA010000000
VERSION     DAZUKA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.302"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8PN_003029"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8PN_003029"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4863098.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          470 a          181 c          225 g          479 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgag
//
LOCUS       DAZUKA010000296         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000296 DAZUKA010000000
VERSION     DAZUKA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.303"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8PN_003030"
     rRNA            <1..1355
                     /locus_tag="ACG8PN_003030"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZUKA010000297         1330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000297 DAZUKA010000000
VERSION     DAZUKA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.304"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG8PN_003031"
     CDS             <1..343
                     /locus_tag="ACG8PN_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4863099.1"
                     /translation="RLQKYIDEGATVILDLDDGVGEYSKMGVCSLDTSFRLLLLDKEQ
                     RKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPSGSLDSHVQLVD
                     LRPEKFSGIKQ"
     gene            complement(457..1086)
                     /locus_tag="ACG8PN_003032"
     CDS             complement(457..1086)
                     /locus_tag="ACG8PN_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4863100.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1220..1292)
                     /locus_tag="ACG8PN_003033"
     tRNA            complement(1220..1292)
                     /locus_tag="ACG8PN_003033"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1257..1259),aa:Thr,seq:ggt)
BASE COUNT          448 a          207 c          233 g          442 t
ORIGIN      
        1 aagattacaa aaatatattg atgaaggcgc aacagttata ttggatttag atgatggcgt
       61 tggggaatat tctaaaatgg gtgtttgctc tttagatact agttttcgtt tacttcttct
      121 tgataaagaa cagagaaaaa atgattataa actccaatta gatagtgata ttggggatgt
      181 ttatataaaa gattattcaa aaatgtacat ggatgaacat atgacccttt ctttagatcc
      241 acgtctaggt gtatttactc ttgaaagccc aagtggttct ttagacagtc atgttcaatt
      301 agtagatcta cgaccagaaa aattctctgg aataaaacaa taactttctc tctattgtcc
      361 ataaaactag tctcaatcaa gctgtattga aaaaacaaga caacattctt accatggtaa
      421 ataaaaagac gacacatttt gtcgtctttt tttaaattaa ctattttcga taaaagatcg
      481 catagcttgt gccattattt ctggcttttc tctattcaat tcatgttttc cgtttttaat
      541 aaagagtagc cggccattcg ttgaaagttt atataaacgt tttgctgctg gtttattcgc
      601 atgatctttg gtaccgcata aaatcaattt aggaagtcgt gatgtacgaa gctgtttttt
      661 gcagtctaat gtttcaagtg aacgaagtag ggtaagggtt tgttttttag atagacctaa
      721 tttttcaaat atcttagaag gtacgcagta aaaataaata ttctggaaag taagcaataa
      781 gcgattagga gactcaaatt gtggagcgac taaaacaacc ttttttaaat atggagaagg
      841 agttgcaaga tagtttaaag caataacagc gcctaaagat aatccacata aaataaaagg
      901 ttcctttatc tgattcaatt gtacttcgat gtatttggtt aagtcgtcta aagtagcagt
      961 gtctgtgctg tttaaatgat tgaacaatga aagacttttg caagaataac tattgggtag
     1021 ataagagatt acatctttcc atgattgttc gtcttggcct agaccatgca aaaaaacaat
     1081 tttcatattg ctctcctttt tatagttatc atatagactt tctttgtact attaaaatat
     1141 aaacattcat ttcttacgtc ataaaataaa aaaacagcgc agacacattg ttaaaccaac
     1201 atgttgcact gtttaaggaa gccacttgcc gggcttgaac cggcgacctc ttccttacca
     1261 tggaagcgct ctaccagctg agctaaagcg gcattatcat taatacaaat aataggataa
     1321 cgaatattaa
//
LOCUS       DAZUKA010000298         1328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000298 DAZUKA010000000
VERSION     DAZUKA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.305"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..517)
                     /locus_tag="ACG8PN_003034"
     CDS             complement(<1..517)
                     /locus_tag="ACG8PN_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4863101.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFL"
     gene            complement(627..1196)
                     /locus_tag="ACG8PN_003035"
     CDS             complement(627..1196)
                     /locus_tag="ACG8PN_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4863102.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          485 a          255 c          174 g          414 t
ORIGIN      
        1 acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga
       61 aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca
      121 agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc
      181 ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt
      241 ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat
      301 aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat
      361 attcatctcc tacaataatt agagactgaa gaaatttgta aatactgtaa tatccataga
      421 ctgttaatac gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt
      481 tagggacata actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt
      541 tcttctctta ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta
      601 caaacagata aaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca
      661 atcaatacac caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata
      721 cctaccgcat agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa
      781 tcttgacgaa aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct
      841 aattgcatta gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat
      901 agaataatat cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc
      961 ccgcccatta acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt
     1021 ctagggtagt tatagctaat ataaacaaat atgattccag ataataatcc aaaaatcccg
     1081 ggggatatgc tgtgaccaaa cgccccacca atactactaa ccattattga aaaaaagtaa
     1141 attactaggg aaataactgt agctaaacca ataaaaatat aatcttttgt tttcatatat
     1201 attctcctca attctatttc gttatgtcat taaaaatctg ttctatcaaa gttgaatctt
     1261 ctactctaag ttttatatct ttttttattc caccattttt taaataaata attctattgc
     1321 atgtttta
//
LOCUS       DAZUKA010000299         1267 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000299 DAZUKA010000000
VERSION     DAZUKA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.306"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..523)
                     /locus_tag="ACG8PN_003036"
     CDS             complement(38..523)
                     /locus_tag="ACG8PN_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFS4863103.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(525..>1267)
                     /locus_tag="ACG8PN_003037"
     CDS             complement(525..>1267)
                     /locus_tag="ACG8PN_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863104.1"
                     /translation="SEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT          395 a          267 c          130 g          475 t
ORIGIN      
        1 aataacatgt cattagatag tagacagttc caactcttta tttacctttt ttgtatgctg
       61 cactgtcaat ccaagttcct aaattgttgt atccatcatc acttatataa tcatattggc
      121 catcaataat ttcgtcaaac cacaaatatt ctccatctgg tttttgccca attattttaa
      181 ttactgattg actagtagtt gtcatctggt taaaatcttt tattttacct atattaacgg
      241 ccaaaatagc atttcctcgc ttataactct cctggatttc atatttaaca tattctcgat
      301 caagagtttc tttacctatc agcaccacag tcactgatgt tccaattaat tgattatcta
      361 tccagttttt tatagcaata tctccattac gtttaacttt ctcaaattca gccttatcta
      421 caaaaccagc ttcctttgtt cctcgcaaag tccagctgtt tctaactacc attgctcttg
      481 ttacatctgc ataatggaaa ctaaaaaaaa cccttctcac catattaatc ctcctgaata
      541 ctatttatta aactgacaat ttgttcaact tttttatcta cttctccttc agtatctaat
      601 ttttctagcg cttgaaattt actttttaag gaatcatcaa aataatcact gaaattctct
      661 tgaatcttat caaacaaaac cttacttgca cctttggtaa atccaatcgg aactactata
      721 ttttcatttt gaacagataa ttcaaattct tcaatcattc catctgctgt aactatttca
      781 cctgattcaa gtttgtttcc aaaaacaaaa atactaatcc cagactctgc aataatgttt
      841 tctcgatatc ttctccactc atgaggcata tcaaggggaa gtggtcttaa agaaagtacg
      901 tcttgtatgt tattttttct taaatttctt ctctgcttta gaactcctga aacaatacta
      961 ttaccaattc caagtccgta acctgatact attctataac cggattcaga aagcctatat
     1021 gctaatttct cgacaaattt taatcctttc gattcagatg taaatggagt ataatcaaca
     1081 gcacttcccg aaataaaaat agtttttctc aaatactttt ttctaatact tttcaaaata
     1141 tcagtaatct cagaataatc atctacttca acaacgttta tcgaaaatct ggacaaatca
     1201 ttgatttgta gtcttctttt tgtcagttca taattatatc tctcttgatc aaaaaaatcc
     1261 tcagaat
//
LOCUS       DAZUKA010000300         1260 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000300 DAZUKA010000000
VERSION     DAZUKA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.307"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACG8PN_003038"
     CDS             complement(93..1136)
                     /locus_tag="ACG8PN_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4863105.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          433 a          251 c          238 g          338 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
//
LOCUS       DAZUKA010000301         1231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000301 DAZUKA010000000
VERSION     DAZUKA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.308"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG8PN_003039"
     CDS             complement(<1..270)
                     /locus_tag="ACG8PN_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4863106.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYT"
     gene            complement(276..506)
                     /locus_tag="ACG8PN_003040"
     CDS             complement(276..506)
                     /locus_tag="ACG8PN_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863107.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(765..878)
                     /locus_tag="ACG8PN_003041"
                     /pseudo
     CDS             complement(765..878)
                     /locus_tag="ACG8PN_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          432 a          201 c          185 g          413 t
ORIGIN      
        1 agtatacttt atatagtcat aacgccgatg atcgcttcga gcgtctagca catcagtcac
       61 gatatcaaac aaaaaatata cgtctttctt cattctagtt ttcgcagcag ggattttaaa
      121 gtagttctta ttagaatagt agagattaat taataagcta tcttcgattg ctaaaaagaa
      181 aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag gatcaaattt
      241 aaaccataat tgcgtctcat taaaaagcat aaccattact ccgatctatt tttaaactta
      301 gtctctacct ctaatatata tcgagttttt attttgcctt cagagaatac tgtttctttt
      361 tttgcagtta caggttgttt attttcggaa aaagctaata tagctaaaat tgaaacatcc
      421 atctggaatt tgtctttttc gttgctttgc tcataaaaat ctgcatattc atcactaata
      481 ttttttctaa taaattcttc catcataaag atcacctcga aaagagtata cgaacaaacg
      541 ttctttttgt aaaggtggaa tttattatac taaataaata ggtgaacaag tacttgtgcc
      601 aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa acaaaaagaa atcctattat
      661 gataacattt cttataataa caaacacaac agaagacgtg taatagttag tcaggaacgt
      721 acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta
      781 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
      841 agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg
      901 gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt
      961 caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata
     1021 tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac
     1081 tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta caactttcct
     1141 tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca
     1201 atagcttaga tcctttattc tatgctttat a
//
LOCUS       DAZUKA010000302         1215 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000302 DAZUKA010000000
VERSION     DAZUKA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.309"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8PN_003042"
     CDS             414..947
                     /locus_tag="ACG8PN_003042"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4863108.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZUKA010000303         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000303 DAZUKA010000000
VERSION     DAZUKA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.311"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8PN_003043"
     CDS             <1..1076
                     /locus_tag="ACG8PN_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4863109.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZUKA010000304         1173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000304 DAZUKA010000000
VERSION     DAZUKA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.312"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..665
                     /locus_tag="ACG8PN_003044"
     CDS             <1..665
                     /locus_tag="ACG8PN_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4863110.1"
                     /translation="YSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMG
                     SYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYL
                     SWFESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALF
                     TIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVG
                     VI"
     gene            665..>1173
                     /locus_tag="ACG8PN_003045"
     CDS             665..>1173
                     /locus_tag="ACG8PN_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863111.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          414 a          156 c          254 g          349 t
ORIGIN      
        1 aatattccat gcagtgttta catcgacaaa cagccaattt cattatattt aaaggagaag
       61 acttgattgg aacaactcaa gacttctctt attttgtcat caatgctgtt tcaaaaacag
      121 aaggagaaat gggttcttat tggcaatcaa ttgaagattt aattgctgat atgacagatt
      181 ttattaatga aaataaaggc gattttacgg actggatgaa tgaaaggaaa gaagaatttg
      241 atcgctggag agaggaacag gagaatagtt tccaagattg gagagaagga caagaatcag
      301 attacttgtc atggtttgaa tcaatcaagg atattttgaa gtctatcgat ccaggtggag
      361 taatgttagc cgaattaatg gatgcacgtg tagatcttca aggtgtacgc catgaatcga
      421 tttctgaacg tttcctatcc gatctaaatt atttgtatca aaaaatgaaa gcagcacttt
      481 tcacgattga atacggtgaa attgaagtga ctgatattct tcaagacgat ctcttttcag
      541 ataatcacga agtcgaaaaa attgaaacag tagaatttcc aattgaagaa ggagctttga
      601 ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa gttggggtga
      661 tttgatggtt aaagtaaaga gaatgatgga aactgatgaa tctggtgtta aacgtcaatt
      721 tatgccaatt acacatgtct ctgcagtttt aggcttagaa aaaatgatat caggacaatc
      781 aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag ctgatttagg
      841 attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg gtattcttac
      901 tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt tgccgattgc
      961 tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta ctgaagaagg
     1021 tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa aaggaaaatt
     1081 agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag atggaacaat
     1141 tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAZUKA010000305         1155 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000305 DAZUKA010000000
VERSION     DAZUKA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.313"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..487
                     /locus_tag="ACG8PN_003046"
                     /pseudo
     CDS             254..487
                     /locus_tag="ACG8PN_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(535..654)
                     /locus_tag="ACG8PN_003047"
                     /pseudo
     CDS             complement(535..654)
                     /locus_tag="ACG8PN_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          433 a          211 c          153 g          358 t
ORIGIN      
        1 aattcatagg aggattcaag atgtctcata ctctgcttat caaagatacc ctaaatattc
       61 tagacctaaa tatcaatatc catttcgagg aaaattgttt aactaaagaa aaatacaaag
      121 gacaaatttg cttgatttac aaagggactc ttcagtacac accagaagaa tatccacatc
      181 ttccaatata ttaaaagaac cttcttgaat cattgagtga aagaaaagag attcgtcact
      241 atttgaggac tgtttaacta gagacattag cgatctgcct gaaacctata caaccacttt
      301 acgaacgttt aaaaattttc tgccacagat tcaaaatgcg cttaactgca gctattcaaa
      361 cagaccattg gaatgtttaa acaaccacat caaagtatta aaacgcaata cgtatggctt
      421 tcgcagcttt tacaatttca aactacggat catgattcgt catggcaaaa catttttaac
      481 caaataaaca gaagaatgtg ggataaatct aaaaatcagt tttgctacag actctcgagt
      541 aaacggcggg agcagaagca actccttcgg aaataagccg aaattcacaa aaatttgaaa
      601 agcaattttc gtgaatttct tcttatttct cggagttaaa cacttctgtc ccagcctctt
      661 ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga gcttcttttt
      721 tgaatactat attcgttctg tttttcatct tcaaatacga actcatccgc agaaatattt
      781 gtcacaactc catgaatctt cttttcatta ccacctatat accacaggat tcctacacag
      841 aaacaacaaa aaaatagcat aaaataggtt ttctttattt tatatccctc cttttagatt
      901 aaaagctcac agaaaacgct ttctaaaaag acaatagaat aatcataatt tttcattttg
      961 tattatttat acaaataata cgcatgttac tgaaaaatga tatggttccc tactttttga
     1021 gataagttaa ataaaatctg tcatcgataa aacagctagt acagtaatga taaatagtaa
     1081 gaacttaaaa tattttggat ttttattttt gaaataatcg aagaaaagta aaagtgataa
     1141 cgaaatagca aacaa
//
LOCUS       DAZUKA010000306         1149 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000306 DAZUKA010000000
VERSION     DAZUKA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.314"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..906
                     /gene="scrK"
                     /locus_tag="ACG8PN_003048"
     CDS             34..906
                     /gene="scrK"
                     /locus_tag="ACG8PN_003048"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4863112.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          333 a          220 c          279 g          317 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaat
//
LOCUS       DAZUKA010000307         1113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000307 DAZUKA010000000
VERSION     DAZUKA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.315"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..921)
                     /locus_tag="ACG8PN_003049"
     CDS             complement(<1..921)
                     /locus_tag="ACG8PN_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4863113.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQL"
     gene            complement(964..1113)
                     /locus_tag="ACG8PN_003050"
                     /pseudo
     CDS             complement(964..1113)
                     /locus_tag="ACG8PN_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
BASE COUNT          328 a          223 c          160 g          402 t
ORIGIN      
        1 taattgttga gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat
       61 cttgattaac tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt
      121 tacaggtatt cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc
      181 aaatataatc cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt
      241 cctcgtatcc ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc
      301 atctaaaaat aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc
      361 tcctgtatat gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt
      421 ggcatattca ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc
      481 caacaaatcc ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg
      541 aagtaaaatg ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac
      601 atttcgcaga cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc
      661 ataccatttt tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg
      721 taaagaaaag aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa
      781 ttcattcaaa tattgagaga caatattacg actaacattt aatttttcag atattttctg
      841 tgctgtattg ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat
      901 gcgttctttt aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag
      961 tatttttcca taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt
     1021 tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc
     1081 caaaatatca acgattgttg caatcagatc ttc
//
LOCUS       DAZUKA010000308         1102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000308 DAZUKA010000000
VERSION     DAZUKA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.316"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..830)
                     /locus_tag="ACG8PN_003051"
     CDS             complement(<1..830)
                     /locus_tag="ACG8PN_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFS4863114.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..>1102)
                     /locus_tag="ACG8PN_003052"
     CDS             complement(901..>1102)
                     /locus_tag="ACG8PN_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4863115.1"
                     /translation="VCIENKILSSESGGQLNKYQIYIERNYSNYKKIFVFLTPDGNEP
                     TNPSWGIITYKNIVEESVKYFV"
BASE COUNT          351 a          199 c          161 g          391 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca cc
//
LOCUS       DAZUKA010000309         1078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000309 DAZUKA010000000
VERSION     DAZUKA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.317"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1078
                     /locus_tag="ACG8PN_003053"
     CDS             <1..>1078
                     /locus_tag="ACG8PN_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863116.1"
                     /translation="FEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSG
                     LYFNSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQ
                     RIVEQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEG
                     LSVLAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNL
                     KLETFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQ
                     DGQSIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLN
                     LIELLISRDTEHKQRKITEEFGVDK"
BASE COUNT          409 a          138 c          209 g          322 t
ORIGIN      
        1 tttgaagaat taatagacaa attagatacg aaagacattg tcaattggct caatcaagac
       61 gaagaattga cagatttatt atcttcggta aaaaaagaat caagtgcaag tcagcaatcg
      121 aactggagtg gattgtactt taattcaaat gagtatgtga aatacatttt gaataaaact
      181 gcatggatta agattgatgg agaaaaatat agcccgagac aaattgtgct atatgaaaag
      241 ctccaggcac acgttccggg attgtatgga gtttctgaac aaagcttaat ggaactcttg
      301 gggcaacgca ttgttgaaca gttggatttc agaaaaaata tggcgatgtt ttcggatgat
      361 gaaataagag ctatattagt tgagttacca aaattcgata aaggcgaaat aagtcgcaga
      421 ttatataatg aacttataag aaataaaaaa ggaatgaatc ctacttattc taccgaagga
      481 ttatcagtct tagccaaaga tggcttcttt tatccaaaca aagaattaag gtatgctgat
      541 aagcgactgc ctaaatcaat agaaaaacaa atgcgtttta tttacgtgcc tgaaaaagct
      601 agtacaacta cgatatatga atggcttggg gttgaacgct tcaaaacaaa tttaaaattg
      661 gaaacatttg agttattgga agattattct gaggatttca aaaaagagat tgatgatatc
      721 aaaactgcgg tattatctac gatagatgac aatagtaaga atgttagttc attaaaacgt
      781 attcagatta ttccatgcag caaaatatct gctaaagatg ttgagcaaga tggtcagtca
      841 atcgttttgg aagattatta ttttgtggaa agtaaaggaa aattctactt aaaattacct
      901 gctaattcta ttgaaattaa tcaattacgt ttgattgata cgttttcttc aacaatcgta
      961 gacatattca agcaggtgtt aaatctaagc ttagatttga atttgattga attgttaatt
     1021 tcccgagata cggaacataa acaaagaaaa ataactgaag aattcggtgt agataagt
//
LOCUS       DAZUKA010000310         1075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000310 DAZUKA010000000
VERSION     DAZUKA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.318"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG8PN_003054"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG8PN_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8PN_003055"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8PN_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          372 a          185 c          156 g          362 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg ctttt
//
LOCUS       DAZUKA010000311         1074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000311 DAZUKA010000000
VERSION     DAZUKA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.319"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..>1074)
                     /locus_tag="ACG8PN_003056"
     CDS             complement(17..>1074)
                     /locus_tag="ACG8PN_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4863117.1"
                     /translation="VYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFI
                     EHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSS
                     RGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKD
                     FLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIR
                     PTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFI
                     CIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIV
                     FWYYLGSISYLYLKKEN"
BASE COUNT          424 a          181 c          131 g          338 t
ORIGIN      
        1 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
       61 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      121 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      181 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      241 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      301 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      361 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      421 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      481 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      541 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      601 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      661 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      721 aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg
      781 taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta
      841 tttttgaaat taatacacta attaaactta aaataataga agtatatcca aacatagctc
      901 caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa
      961 gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag
     1021 atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac acca
//
LOCUS       DAZUKA010000312         1063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000312 DAZUKA010000000
VERSION     DAZUKA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.321"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..462
                     /locus_tag="ACG8PN_003057"
     CDS             52..462
                     /locus_tag="ACG8PN_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4863118.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            476..832
                     /locus_tag="ACG8PN_003058"
     CDS             476..832
                     /locus_tag="ACG8PN_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4863119.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          367 a          159 c          206 g          331 t
ORIGIN      
        1 tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa aatgataaaa
       61 gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat
      121 caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa
      181 aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt
      241 tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc
      301 aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc
      361 ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga
      421 aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga
      481 agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga
      541 acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatttaa gtacagaaga
      601 agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc
      661 tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt
      721 tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg atgaagttct
      781 atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac
      841 tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa
      901 gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat
      961 gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa
     1021 tttacatgat atattcattt ctgttgtttt aagtagcaaa gca
//
LOCUS       DAZUKA010000313         1050 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000313 DAZUKA010000000
VERSION     DAZUKA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.322"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..844)
                     /locus_tag="ACG8PN_003059"
     CDS             complement(35..844)
                     /locus_tag="ACG8PN_003059"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4863120.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          319 a          201 c          183 g          347 t
ORIGIN      
        1 tcacaacctc ttttttgtgg aatctaattc tctattatag tttaagtagt ttttccagat
       61 aaacagctaa accgtcttcc tgattgctca aaggagtgac atcatttgca atgcctttga
      121 gttggtcagt accgttagcc atagctactc cccaacctgc ataatccaac aattccaaat
      181 cattatgctc gtcaccaaag gcaatgacgt ctttttgatc gatagacagg tagttagcaa
      241 tctcttgcac gccttttgct ttttggattc cttttgaaac aatctctaag atggcagtag
      301 gtccacccca tgtccgaaca tcgacataac tgccaaattg acgtgttaat tcagcagata
      361 cagctcctgc aaaacgtttg tctgttcgga caagcaaaga tgtaggattt gtcgtcaaat
      421 ttttaggaga aagcaaattc ttctctcctg gacgagaaga agcaaaaatt ttttcatcaa
      481 agaaatcaaa actgtcaata aaaaaagtat cacgattttc agcagcgata aaatctaaat
      541 ttagctgctt tttttgcgat aagatttcaa aagcgatttc acgattgatc aacgtttctc
      601 tttcaccatc ccagtgctga ttagggatgt gtactaatgc gccgttaaaa ttaaccatcg
      661 gtgttgttaa ttctaattgt tggtaaaact gatggctcat tcggaatggg cgtcctgtag
      721 caatactgac atgatgaccg gcattgattg ctttttttag agtctctttt gttttagatg
      781 taatcatcga atcttgattg agggtagttc cgtccagatc gatggcaatt aattttttat
      841 tcaataaggg tccttccttc ctcaactagc atttgtttta gtctagcata ttttaggtaa
      901 ttaacaagag caatcagcta ttcctaacaa aaactacagt tatggctata taaatagcta
      961 aacataagct aaacacgaac gttaagtatt attttcgctt tttttgttaa aaaaacgttg
     1021 aaaagcgaat gaaacgtcta tataatttga
//
LOCUS       DAZUKA010000314         1035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000314 DAZUKA010000000
VERSION     DAZUKA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.323"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACG8PN_003060"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACG8PN_003060"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAZUKA010000315         1031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000315 DAZUKA010000000
VERSION     DAZUKA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.324"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..569
                     /locus_tag="ACG8PN_003061"
     CDS             162..569
                     /locus_tag="ACG8PN_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863121.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            583..984
                     /locus_tag="ACG8PN_003062"
     CDS             583..984
                     /locus_tag="ACG8PN_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863122.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          388 a          132 c          174 g          337 t
ORIGIN      
        1 ctgattaaat gtcagatatg cttacaaata tacttataaa tatgcttaca aaattagcta
       61 atatttccgt tgacaaagct ttgttactat tgttaaacta aattgactag caaaagtgaa
      121 acataaattt attaaataaa tgtaaaaatt aggagaaatc aatgtcttat agagtacaat
      181 taataatttc agaagatgta gaaagtcagc aatttggcac taatgttatt aataaagtaa
      241 ttaatccagc tctttctatt aatgcgcctt taattcctac tgctttatca ttttctgtaa
      301 cagctgtggt gtctgagata gaggatacag aaaagataaa aatagtagaa attgaagttt
      361 taaacaagaa tgaaaaacaa attttttcta cgggtgaagt atcggtaagc ttgcctcctc
      421 aagttaacga tataaacttt aatataaacg ccagaaatgt cttggttgaa gaagcaggag
      481 agcattatgc tgttgttaaa tttaatggaa ctgagattgg aaggcaaatc tttgatatca
      541 aggtcaacaa accagtggaa aaaaattaag gagacgatac ggatgcttga tatagtacca
      601 tcaaatacac atcggagtgg tagcttaata aattttgctg cgatttttgc ttgcgcatcg
      661 gtaattctag catctccagt tgttgtggat gcaagttcta ctccgaaaac taaaaatgat
      721 aatcaacttg taattactac aaaaaatgag attaacacaa tttcaagtaa tgatggcaaa
      781 atttttgatg tgataaatac ggttttaaaa agaaaagctg aaatcaatat aaaatatgat
      841 gaagatttaa acttgtattt ctttgttatc aaaacaacat ctgaattgtt ttcctcagat
      901 tacgatgttt tggatactct cgataatgta ttatctgact ataaatatat ggggaaatct
      961 gtagtagcaa ctttggggga ataaaatgtt tgactttaat gaatatttat ctatttccaa
     1021 acaaattgaa t
//
LOCUS       DAZUKA010000316         1021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000316 DAZUKA010000000
VERSION     DAZUKA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.325"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACG8PN_003063"
     CDS             2..460
                     /locus_tag="ACG8PN_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4863123.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="ACG8PN_003064"
     CDS             469..696
                     /locus_tag="ACG8PN_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863124.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DAZUKA010000317         1001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000317 DAZUKA010000000
VERSION     DAZUKA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.326"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACG8PN_003065"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACG8PN_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAZUKA010000318          998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000318 DAZUKA010000000
VERSION     DAZUKA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.327"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8PN_003066"
     CDS             <1..649
                     /locus_tag="ACG8PN_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4863125.1"
                     /translation="SIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAIN
                     RVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIH
                     SPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTK
                     QNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            639..>998
                     /locus_tag="ACG8PN_003067"
     CDS             639..>998
                     /locus_tag="ACG8PN_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4863126.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNI"
BASE COUNT          385 a          119 c          188 g          306 t
ORIGIN      
        1 ttctataaaa aataccatga ttaatacatg gaataacatt aaggactctt tctggaatat
       61 tgttactgga attgttaatt ctgctgaaaa tgcatggaca aatctaaaaa atggtgtttc
      121 aaatgctatt aatcgggtaa aagaaatctt taattcgtta agggaagtca atttatttga
      181 aattggcaag aacatcattg atggacttat aaatggtgta aaagaaaagt ggaatgcttt
      241 gaaagatact atcaaaggta ttgcaggtaa tatcaaaaat tggattaaag gagctttaga
      301 tattcattct ccatcaagat ggatgcgtga tatggttggt aaaaatattg ttcaaggtat
      361 tatcgttggt attgataaag agcaaagtaa gttagatcaa accatgacaa atttagtaaa
      421 aacaccgtct gttcaacctg tagtaaatgg aataagtcac ctaccaatta cacaaacaaa
      481 gcaaaatata tctgctagtg aaacaaaaga aattcattta catttaaatg tttatggaga
      541 tttacctgat tcaatggttc gaaagattac tacaaaaatt aaaacagaat tgacaagaca
      601 aatgaaacga gatgctggtg cagtaggagg aacattatat gcaacttaag agaggacaat
      661 tttttattaa tcaacattat tcttctgaat ttaatgtgta tattcaaaac aggcctgctt
      721 ctgtttcagc tagtcgagta attgaattga gagagcgaga aggaaatgat tctatcatta
      781 ttgataaagg ctattacaaa aatgtgacta gaaagattga gtgttactat aaagcacctt
      841 caattgatat ggtgcaggag tgggaagatc gaatcactga atggttagat atgtcgtctt
      901 atagtgattt tattctttac tatgatgaac aatatattta tcaagctgta gtaatagaag
      961 gacctgaatt taaaggaaca agaaaaacag gaaatatt
//
LOCUS       DAZUKA010000319          997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000319 DAZUKA010000000
VERSION     DAZUKA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.328"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACG8PN_003068"
     CDS             <1..418
                     /locus_tag="ACG8PN_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4863127.1"
                     /translation="PKVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDINKD
                     GMAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAKKIT
                     KLLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            437..>997
                     /locus_tag="ACG8PN_003069"
     CDS             437..>997
                     /locus_tag="ACG8PN_003069"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4863128.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRK"
BASE COUNT          365 a          133 c          195 g          304 t
ORIGIN      
        1 cccaaaagta gaatatattt atgaatttga aggtgggaag gggatcacta atcaataccg
       61 acctaacaag ttcgaaacat ttaaagaatt ctattcatta gcaaatactg taacagacga
      121 tattaataaa gacggtatgg cggcgcttct tttaaaaggg acaacttcag ttattttttc
      181 tatttatagt acctctgcaa gaacttcaaa agagaagaaa gacgaagcaa agcagatatt
      241 gagtgatcca gtgatccgag aagccaagca aattgctaag acggattctt cagcaaaaaa
      301 aataacaaag ttgctttaca atatgccagc tccactattt ttatctgctg taaaagttgg
      361 aagcttcgtt gaagtgaaaa tgccagggtt aatggctgct ttgaagcgag tatattagtt
      421 aatggaggaa aatataatga aaagaatttt gcattttcag ggacgaatgg gtttaggtgg
      481 agcagagtct ttcatgatga atttatatcg taaaattgat cgtacaaact atcaatttga
      541 ttttttgatt tatgaagatt atgcagatgt tcaagactat cattcagaga ttgaaagatt
      601 aggaggccgt atttttgttg tacctaatcc taagaaaaat atttttaaat atttaataga
      661 agtgaataag ttactaaaaa aagaatcttt ttctatagtt cacaatcagg tttattttgg
      721 cgggggaatt aatttatggt tagccaaaaa gaatggaatc agacagagaa ttgctcatag
      781 ccatgcaact gaagatggaa aatctcaaaa tattgtaatg aatgtattgc gtaaattttt
      841 aaccaaattg ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag aagctggtga
      901 aagtctgttt caaaaccacc cctttgagat agttcataat ggtattgatt tagagcttta
      961 tagtaaaaat tcagaagcta aggttaataa gagaaaa
//
LOCUS       DAZUKA010000320          983 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000320 DAZUKA010000000
VERSION     DAZUKA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.329"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>983
                     /locus_tag="ACG8PN_003070"
     CDS             <1..>983
                     /locus_tag="ACG8PN_003070"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4863129.1"
                     /translation="EPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITDS
                     EYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQLD
                     DTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTGK
                     KNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVGS
                     IAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFYE
                     NFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          374 a          159 c          189 g          261 t
ORIGIN      
        1 gaaccaggcg attttatcat gagtggcgct ggaacaattg ggagaatttc aagagtacca
       61 aagcaaataa aaccgggtgt attcaatcag gctttgatta gacttagaat aaataaagaa
      121 atcacagatt cagaatattt tattcaattt attcgtgcag attttatgca aagaaaactc
      181 acaggagcta atccaggttc agcaattaca aatctcgtac caatgtcaga agtgaaaaaa
      241 tggatagtac aatttccaat attagaagaa caaaagaaaa ttgggaactt cttcaaacaa
      301 ctggacgaca ctatcgctct tcaacaacgt aagctagatt tattaaagga aacgaaaaaa
      361 ggattcttac aaaaaatgtt ccctaaaaac ggagcaaaag tgccagaaat tcggtttcct
      421 ggatttacgg aagattggga acagcgtaag ttcaaagagt ttagtaagaa gactggaaaa
      481 aagaacacga aagatttaga cttccctgcc tattcggtaa gtaataaagc aggtttaatt
      541 tctcaaacag aacagtttga tggtagtagg ttggatgatt tagaaaaaac aaattataaa
      601 ttcgttgaac caaatgaatt cgcctataat ccagcaagag taaacgtagg atcaattgca
      661 tttaacaatc ttggtatgac ggtcattgtt agctctcttt atgtagttgt gaaaatgagc
      721 gaggacttag ataatgagtt catacttcag tttatcaagt caccaacatt tatcaaagag
      781 gtcaagcgca acactgaagg aagcgtacga gaatacttat tctatgagaa tttcgctaat
      841 atcaagtttc cttttactag gaataaagag gaacaacaaa aaatcggcgc tttcttcaaa
      901 caactcgacg acacgatcgc tcttcatcaa cgtaagttag atttgctgaa ggaaacgaaa
      961 aaaggcttct tacaaaagat gtt
//
LOCUS       DAZUKA010000321          980 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000321 DAZUKA010000000
VERSION     DAZUKA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.330"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACG8PN_003071"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACG8PN_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACG8PN_003072"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACG8PN_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          291 a          185 c          172 g          332 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt
//
LOCUS       DAZUKA010000322          946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000322 DAZUKA010000000
VERSION     DAZUKA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.331"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..925
                     /gene="thiD"
                     /locus_tag="ACG8PN_003073"
     CDS             110..925
                     /gene="thiD"
                     /locus_tag="ACG8PN_003073"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4863130.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          342 a          150 c          216 g          238 t
ORIGIN      
        1 ccaagaaaag gtagtggtat atttactgag ggatactttg aaaaacatat gatagaaaca
       61 gtaacagtat tatgaaaaat ctttttataa aaatggagga atcgatttga tggaagatca
      121 gtatgtacaa gcaatgacga tcgcaggatc agatagcgat ggaagcgcag ggatgcaagc
      181 agatatgcat acattcttta caagaaatgt ttacggtgtt tctgtcatga ccgcatgtgt
      241 tgcaggtaat tcttacggaa tcggagcaag tgtgacattg ccaacagatt ttatcgacaa
      301 agaatttgaa ctgattgcga aggactttca aatacgtgca gctaaaacag gtatgctagc
      361 agattcaaag ttgattgaga cagttgtaaa aaattaccaa aaatatgatt ttggaccatt
      421 agtcgtggat cccgtaatcg taacgaaaca tggaaatctt ttactagaag aatctgcttt
      481 gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta ctgacaccta atttttatga
      541 agcagaaaag ctgactggac ggacattagc agctgaagaa gattttgtgc aagcagcaaa
      601 agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa ggacatcatg ctaaaaatgc
      661 gcagcaagaa acagtcaatg actacgtatt gttcgaatca ggaaaatcat tttggttaaa
      721 tgcaccattt tatcctaccg accggatcaa cggaactggc gattcgctat ctgcatgtat
      781 tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg atccgattgg caaaagacta
      841 tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac aaatatggcc ccatcaatca
      901 ttgggcagct ggtgaattag agtaaaagaa agaagttgtg aaaaag
//
LOCUS       DAZUKA010000323          945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000323 DAZUKA010000000
VERSION     DAZUKA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.332"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8PN_003074"
     CDS             complement(53..787)
                     /locus_tag="ACG8PN_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4863131.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          328 a          192 c          180 g          245 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaa
//
LOCUS       DAZUKA010000324          945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000324 DAZUKA010000000
VERSION     DAZUKA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.333"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..574
                     /locus_tag="ACG8PN_003075"
     CDS             35..574
                     /locus_tag="ACG8PN_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863132.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            571..909
                     /locus_tag="ACG8PN_003076"
     CDS             571..909
                     /locus_tag="ACG8PN_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFS4863133.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT          343 a          127 c          177 g          298 t
ORIGIN      
        1 tcctcaaaat ttaatgaatt aggaagtgat tactatgaaa aaattattta gcgcactttt
       61 tattctgtta tttggcttta ttggtattgg gacttctgga aatatagtta aagctagcac
      121 gatggttgaa caaacaagtg aaatggcaaa aacttttagt caattaacac cagaagaaca
      181 aagttacttc aaatccaaag gatttagtga taaagatact ttttacactc aaaagataga
      241 gcctcaagat ccgaaaacac gtttttctgc aaatgtattg cggttaaccg catctactaa
      301 aaaaattact aatacgcagg ggcggacaga atatattata acaagtccca attcggcgat
      361 gtatcgtatt tctacacgct tgagtttagg tagtgcaaaa tcatatactc aaacagtaaa
      421 tctagctaat tcttatggtt atggtggaga aatgtatttt acatttactg ggaaaagaca
      481 atattacagt gtaaaattag ctatgcaagt ttataataca tttggtgtag ggtcagtctc
      541 ttgcactgcg ggaggattga caataggaaa atgataattt cttactatgt cttaaataga
      601 agtgatgatg aaggactctt cgtttttcaa tcattacatg aggatacgat gctaaaagag
      661 ttttataatc ggattaagaa taaacagata gaaacaaatt tatatttttt aagtgactct
      721 atcttgcctt ttattagcaa ccctaaaatt cttaacttgt tgcagacaaa agatttgaaa
      781 agtttaccga ttattgtcat tgatgaggaa attgtaaaat atggcgctta tctaagcgta
      841 gaagaattag aaaaattaac taacattgga atgagtattc aggaggataa tggggtacat
      901 ggaacataga atgaatatgc acagttgaga acggaatttg acatt
//
LOCUS       DAZUKA010000325          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000325 DAZUKA010000000
VERSION     DAZUKA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.334"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8PN_003077"
     rRNA            <1..>943
                     /locus_tag="ACG8PN_003077"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUKA010000326          929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000326 DAZUKA010000000
VERSION     DAZUKA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.336"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..301)
                     /locus_tag="ACG8PN_003078"
     CDS             complement(71..301)
                     /locus_tag="ACG8PN_003078"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4863134.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(395..733)
                     /locus_tag="ACG8PN_003079"
     CDS             complement(395..733)
                     /locus_tag="ACG8PN_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863135.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          293 a          193 c          103 g          340 t
ORIGIN      
        1 tattaacagc aaagatatcg aaaaaacaat aacataatat gtgtcaaaat ttttcataat
       61 aagaactccc ttactgttgc atactaacat aatccaaaaa ctcttctact tcagctaaag
      121 acattccact ttctttagtc caaaaaacag ttttttctat cgatccttct ctgtaaactg
      181 taaatgtcgg agtcgagtta attgagtagc gactcttgaa ttctttccac tcattttcat
      241 cttctctaac ttttgctaca ttcacgtaat aaatattttc ttttcccttt tctaccagca
      301 tatcgaatag cttcctgctt ttgatgatcg atttcttgtg ctacatctag taaattttca
      361 tcaatttcta tatcataaag tatttcacta gtgtctacat ccacaatata tactttttat
      421 cagtagcaat ttttgagtaa tccgttaaaa tgatctcagt ctcgtcactt tcatcagtat
      481 aaatatacgt taacaatgaa ctcacctttt caatcggtgt tagctctaag cctactgtat
      541 tttttactat tgagtcatta tttaccacca taacttcgta ctgtatatac catcctattt
      601 ggtttccatc aatatcagta attggctcac tagctaatgg agtctccgga acaatatttt
      661 cctgatattt cttaaactct tctgtagcac gaaaaaggtt atcatgttcc tcacttgaaa
      721 ctggttgagt catagaaaat gttacttggc taaacagagg gaaaataaac actacaatca
      781 acaaagtata aaaaaaagcc cttctttttc attttttatc ttccaatcac aaattgaaaa
      841 acttattcct gcaactttga gtctagtatc acaatcgact gactacaact attcatttac
      901 attctacttt aagttcctcc ttaaccatt
//
LOCUS       DAZUKA010000327          922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000327 DAZUKA010000000
VERSION     DAZUKA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.337"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>922
                     /locus_tag="ACG8PN_003080"
     CDS             <1..>922
                     /locus_tag="ACG8PN_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4863136.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLNN"
BASE COUNT          280 a          187 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca ac
//
LOCUS       DAZUKA010000328          913 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000328 DAZUKA010000000
VERSION     DAZUKA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.338"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..817)
                     /locus_tag="ACG8PN_003081"
     CDS             complement(<1..817)
                     /locus_tag="ACG8PN_003081"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFS4863137.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINML"
BASE COUNT          361 a          150 c          126 g          276 t
ORIGIN      
        1 ctaacatatt gatagaaaat atatatgagc ttaagctgat aacttttttt tcgtaaatat
       61 tcatttttga taatttatga actaaaaaaa gatttgctga aaaaaagaga gcaaacatca
      121 ctacgtgaga aatcgatgta ctgtttctat caaaatatgc atcatacttc gtatttataa
      181 atattgaact gatataactt tgtccaatag agaataggag caagctaagg attgtaacat
      241 atccagtaat ctttttatta atttttacaa taaaaaaagg gagataaaaa tatgcaagat
      301 tatggaacag tccagccact aagataaaaa aaatatactt aaatatagct ttgctgcctc
      361 gcaatttagt tagatgataa aaagctagta atatgaatat caatgcgcta aacatacgta
      421 cttgtattgc ttgtgaagta aaaccaaaaa taaaaaacaa gaacaaaact aaaaaaacat
      481 tagttgtgaa taatctgatg acatacaata aagctgagag taagacagta gaaataatca
      541 ataaaaaaat ctgatactga ctggaaataa aggtacatac gtacataaca gctctgaatc
      601 ccggttcaat actatctgaa ctaaagtcca taccaaattg tgctatccca ttatacaaga
      661 cagaatatgc ataataatca gcgttttgtg tgttaaatgc catgatgatc cacattatca
      721 aaagtagcac cccagacaaa atcttgttcg atgtactcca ttttttttgt gaaacactct
      781 tttcttgatt aatctccaat gacgcctctg tatccatctc gaacccccct tatagccgct
      841 ctaaatctac tgagcttatc agtctcaaaa gctattgcaa taaaaaattt caataatctt
      901 ttattcataa ggc
//
LOCUS       DAZUKA010000329          892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000329 DAZUKA010000000
VERSION     DAZUKA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.340"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..352
                     /locus_tag="ACG8PN_003082"
     CDS             <1..352
                     /locus_tag="ACG8PN_003082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863138.1"
                     /translation="DESDLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKE
                     IKSLSEDMVKYQKVFNCYKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNV
                     LNEVDESLKKYFTK"
     gene            complement(677..823)
                     /locus_tag="ACG8PN_003083"
                     /pseudo
     CDS             complement(677..823)
                     /locus_tag="ACG8PN_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          295 a          140 c          177 g          280 t
ORIGIN      
        1 tgatgaaagc gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc
       61 agtggaagaa gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca
      121 agtaattaaa gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa
      181 ttgctacaag aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag
      241 taaaaataga attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg
      301 aaatgtcctc aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt
      361 tttgttgaca tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta
      421 tttttaatgt acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta
      481 tatggtgtct ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg
      541 taataataaa ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat
      601 tctttttgtt tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca
      661 gacggctcat aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc
      721 gcagtagcgt gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt
      781 tggtggctag agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc
      841 tgtgaatggg tgtggtaact ttattctaca gaagggactt ccttttttct at
//
LOCUS       DAZUKA010000330          884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000330 DAZUKA010000000
VERSION     DAZUKA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.341"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..807
                     /locus_tag="ACG8PN_003084"
     CDS             67..807
                     /locus_tag="ACG8PN_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863139.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          324 a          162 c          182 g          216 t
ORIGIN      
        1 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
       61 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
      121 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
      181 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
      241 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
      301 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
      361 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
      421 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
      481 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
      541 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
      601 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
      661 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
      721 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
      781 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
      841 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaa
//
LOCUS       DAZUKA010000331          858 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000331 DAZUKA010000000
VERSION     DAZUKA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.342"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..>858
                     /locus_tag="ACG8PN_003085"
     CDS             139..>858
                     /locus_tag="ACG8PN_003085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4863140.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIA"
BASE COUNT          349 a           98 c          148 g          263 t
ORIGIN      
        1 ccgttgatga aaatgagatg ttgaaatttt atcagtgtaa taaaaacata aatgaaaatg
       61 ttagaaaaat ttacgccact atattctctg agcaagtatt gaaaatgaaa tggaatgaag
      121 tgtacaaggt gatgaacgat gaaaaataaa attgggataa ttattgttac ctacaatcca
      181 gataataaca taaagttatt agtggatacg ttaatacaag agaaacagaa caaacttatt
      241 atcgtggata acggctcaga taatattaac tcttttcaat ttgaaatgat ggaaaactta
      301 attttaaaaa aaatgaatga gaacattggg cttgcgacag ctcaaaacat aggtattgaa
      361 attgccaaaa aagagaaatg tgattatatc cttttactag atcaagatac catcatacaa
      421 aagaatcttt ctttaaaatt attagaagag tttaccaata ttgaagcgaa agataaggac
      481 attgggatta ttgttcccaa ttactttgag aatagcaatg atgaatctgt ctcttttacc
      541 tgcaaaaatg agagagggag atacgtaaaa aaacactttg cttcaactag tggttccatg
      601 aaaatagcat ttgcaaattc ctctggaatg tttttcaaaa gtgaattaat tgacgatgtt
      661 ggaaatatga aggatgaatt ttttattgat cagattgatg ttgaattttg taaaagggtt
      721 gcaaataatg gctataacat ttatgtaaca aatcaagtct atttttatca tcacgttgga
      781 gatggagaaa atttaaaaat aggatcaaaa aaaattaaaa tagacaatca taattcaaca
      841 cgaaagtatt ttattgca
//
LOCUS       DAZUKA010000332          843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000332 DAZUKA010000000
VERSION     DAZUKA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.343"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..803
                     /locus_tag="ACG8PN_003086"
     CDS             <1..803
                     /locus_tag="ACG8PN_003086"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4863141.1"
                     /translation="INKKETTFMNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFI
                     SKGPFPSPRKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLF
                     VQDENDANEIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKH
                     STEKTLYFTGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKA
                     IPDSTIIAVHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT          320 a          117 c          160 g          246 t
ORIGIN      
        1 atatcaacaa aaaggagaca acgtttatga atatacatca cattcgtaat gcaacagcta
       61 tcttagacta tggaggtaaa aaatttttaa ttgatccaat gttagcggaa aaaggaacac
      121 ttccaccatt tatttcgaaa ggtccttttc catcaccaag aaaagatcaa atgaatcctc
      181 tagttgattt gccaatgcca gtcgaagata ttatcaacgg tattgatgct gttattgtaa
      241 cacatttaca tctagatcat tgggacgata tagcgaaaga ggtcctacct aaagaaataa
      301 aattatttgt gcaagatgaa aatgatgcga atgaaattcg actatatggt tttaaaaatg
      361 tggaggtact acgagaagac acagtttttg aatctataca attggttaaa acaaaaggca
      421 aacatggcag aggagaaatc ttgaattatg taggtaatgt ttgcggtatt gttttcaaac
      481 attcgactga gaagacacta tactttacag gagatacagt ttggtatgaa gctgttaaac
      541 aaactattga tcagtataat ccagaaatca tagtagcaaa tgctggagat aatcattttt
      601 tagttggaga atctcttatt atggggaaag acgatttata tgaattgcat aaagcaatac
      661 cagattccac aattattgcc gttcatatgg aagctgtaaa ccattgggga ctgtcaagag
      721 aagaattaaa gtctttcaat aaagaaaaag gaatttccga aaaagtttta gtgcctgaag
      781 atggagacaa catctttatt taaaaaaata aaagattatt aataaaggga tagatgctac
      841 tgt
//
LOCUS       DAZUKA010000333          841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000333 DAZUKA010000000
VERSION     DAZUKA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.344"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..112)
                     /locus_tag="ACG8PN_003087"
     tRNA            complement(40..112)
                     /locus_tag="ACG8PN_003087"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:His,seq:gtg)
     gene            complement(115..188)
                     /locus_tag="ACG8PN_003088"
     tRNA            complement(115..188)
                     /locus_tag="ACG8PN_003088"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Trp,seq:cca)
     gene            complement(195..275)
                     /locus_tag="ACG8PN_003089"
     tRNA            complement(195..275)
                     /locus_tag="ACG8PN_003089"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Tyr,seq:gta)
     gene            complement(277..352)
                     /locus_tag="ACG8PN_003090"
     tRNA            complement(277..352)
                     /locus_tag="ACG8PN_003090"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(388..460)
                     /locus_tag="ACG8PN_003091"
     tRNA            complement(388..460)
                     /locus_tag="ACG8PN_003091"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Asp,seq:gtc)
     gene            complement(470..542)
                     /locus_tag="ACG8PN_003092"
     tRNA            complement(470..542)
                     /locus_tag="ACG8PN_003092"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(507..509),aa:Val,seq:tac)
     gene            complement(558..629)
                     /locus_tag="ACG8PN_003093"
     tRNA            complement(558..629)
                     /locus_tag="ACG8PN_003093"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Glu,seq:ttc)
     gene            complement(644..733)
                     /locus_tag="ACG8PN_003094"
     tRNA            complement(644..733)
                     /locus_tag="ACG8PN_003094"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Ser,seq:gga)
BASE COUNT          232 a          233 c          191 g          185 t
ORIGIN      
        1 ccttgcctta ccgcttggct ataccccaag aataaaaagg gcgaatgatg ggaatcgaac
       61 ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc gccatggcag
      121 gggcagcagg aattgaaccc acactaacgg ttttggagac cgctgttcta cctttaaact
      181 atgcccctat aaaatggagg agagtggatt cgaaccactg aaccctaagg aacggattta
      241 cagtccgtcg cgtttagcca cttcgctact cctccatggt ggcgcgggac agaatcgaac
      301 tgccgacaca cggagcttca atccgttgct ctaccaactg agctaccgcg ccatatttta
      361 aaattattaa ttgaataaca agaacgacgg ttccgacggg aatcgaaccc gcgatctcct
      421 gcgtgacagg caggcatgtt aacccctaca ccacggaacc agttcattat ggaggttaac
      481 gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaacct
      541 ccaacattca attaaaatga cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg
      601 cggtgtctta accgcttgac caacgggcca ggtaaatttg ctacggagag taagggattc
      661 gaacccttga gacagtttgg caccatctac atgatttcca atcatgctcc ttcggcctct
      721 cggacaactc tccagagtga aagaagctca atactctttc tcaaaatgag aactccggca
      781 gtaggactcg aacctacgac atcatgatta acagtcatgc gctactacca actgagctat
      841 g
//
LOCUS       DAZUKA010000334          835 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000334 DAZUKA010000000
VERSION     DAZUKA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.345"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..310)
                     /locus_tag="ACG8PN_003095"
     CDS             complement(<1..310)
                     /locus_tag="ACG8PN_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863142.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     E"
     gene            complement(317..>835)
                     /locus_tag="ACG8PN_003096"
     CDS             complement(317..>835)
                     /locus_tag="ACG8PN_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4863143.1"
                     /translation="LGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNKLVDELTFYFD
                     TVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVA
                     INSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVA
                     GIAAAAASCALE"
BASE COUNT          277 a          175 c           90 g          293 t
ORIGIN      
        1 attctcccca aaaaggaact ttttctagtg aaaacttggt aatctctttg taattaattc
       61 ttgttaaaga ataattaaac acttcatttt tacttgttat tttttcattg actataacta
      121 gttcatctgg taaaaaaatt aaccaacaaa catcgacaaa cagtaaatga tataatccat
      181 ttccacctcc aagcatattc aatactggtg aacatgcctt agaaacacga atatagttat
      241 ggtttaaata gatattttct ttaaacattg cttgtttaaa agccatatgc tcttgttcta
      301 aacttttcat aaacttttac tctaatgcac agctagcagc agctgctgca attcctgcta
      361 caatatttac cttagctaac tctttagctg cagcttttcc taattgtttt tctgcaattt
      421 ttaataataa atccgcagct ttttcaaaat taaaagcttt taatgcttca ataaaatctc
      481 cctgcgtacc tatagcccaa gccactcctc ctccaggaag agaattaatg gctacacatc
      541 cagcatatcg tttccaatca actttctcca ttctaggaac aataatatgt tcataaaaga
      601 ctcttgctgc tcttccttgt tcgttatctt gactgattcg ttcacgaata gcaccaaaat
      661 cctttatatg gtatacacca tgttcgtcta aaaaaccaac tgtatcaaaa taaaaagtta
      721 attcatctac taatttattc tttatttcta aatcaactgt attcactttt tctgtggcat
      781 acacacttgt tccagtacta acaaaagtac ttaaacctaa taagaaaaca cctaa
//
LOCUS       DAZUKA010000335          800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000335 DAZUKA010000000
VERSION     DAZUKA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.346"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..164)
                     /locus_tag="ACG8PN_003097"
     CDS             complement(<1..164)
                     /locus_tag="ACG8PN_003097"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4863144.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYN"
     gene            complement(416..>800)
                     /locus_tag="ACG8PN_003098"
     CDS             complement(416..>800)
                     /locus_tag="ACG8PN_003098"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFS4863145.1"
                     /translation="LNSSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESIIEQISK
                     YREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPYVLWKSG
                     HAMKEVTTLGNNVYKSAKLRIISHY"
BASE COUNT          254 a          157 c           94 g          295 t
ORIGIN      
        1 ttattataat cgtacagtat cgttaatcga tcttctttta attggacatc ataataatca
       61 ttgagattat caatcgctac aatttgattt tcagtttcct tgagtaattt tctagacaaa
      121 tgaaaaccaa taaatcctgc tccacctgtt attaatattt tcatttttat tcatccacct
      181 ttttctgaaa ggaaccaata gggttaaacc caaaattgct ttcttcttta tactcgtatt
      241 acatttatta tttaggcata ccccataatt taagatataa cgaagtttat gatactatgc
      301 gtcttctcca ttcactcaaa aaatataact taatttttta tgactttaaa aaggaaatct
      361 tctatctcca aatatttact aggatttgaa agaatagttt agaaatcatt tagggctaat
      421 aatgacttat gattcttaat ttagcgcttt tatatacatt attgcctaag gtagtcactt
      481 ccttcattgc atgtccggac ttccacaaca cgtaaggaaa tgcgagctga tctcgcccac
      541 tttctgagtt caaatactct ctccaccaca aatctagaag attctcagcg attgaattat
      601 ttaaatcaac taaaataacc gtggcttcag ccaatccaaa attatcagga aatccttctt
      661 ctctgtactt actaatttgc tctataatac tttccttatt acctttgcca actgcaatta
      721 atgcatctgc ctcagaatat aaagattttc tttcactatg tttatgcata cttaacccaa
      781 gtttggaaga actgttcagt
//
LOCUS       DAZUKA010000336          791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000336 DAZUKA010000000
VERSION     DAZUKA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.347"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8PN_003099"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8PN_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4863146.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          208 a          176 c          122 g          285 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta c
//
LOCUS       DAZUKA010000337          770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000337 DAZUKA010000000
VERSION     DAZUKA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.348"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..66)
                     /locus_tag="ACG8PN_003100"
     CDS             complement(<1..66)
                     /locus_tag="ACG8PN_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4863147.1"
                     /translation="MIFPKVNEASLNKGSFFHIYEN"
     gene            417..716
                     /locus_tag="ACG8PN_003101"
     CDS             417..716
                     /locus_tag="ACG8PN_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4863148.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          293 a          101 c          133 g          243 t
ORIGIN      
        1 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
       61 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
      121 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
      181 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
      241 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
      301 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
      361 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
      421 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
      481 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
      541 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
      601 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
      661 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
      721 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag
//
LOCUS       DAZUKA010000338          705 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000338 DAZUKA010000000
VERSION     DAZUKA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.350"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..425
                     /locus_tag="ACG8PN_003102"
     CDS             <1..425
                     /locus_tag="ACG8PN_003102"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4863149.1"
                     /translation="KMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPE
                     LLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQE
                     WAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            458..>705
                     /locus_tag="ACG8PN_003103"
     CDS             458..>705
                     /locus_tag="ACG8PN_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4863150.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRAL"
BASE COUNT          279 a           74 c          129 g          223 t
ORIGIN      
        1 taaaaatgaa ttctgattct tacttgattt tagtaggtga agggtcactt agaaataaac
       61 tgaaagccaa agcagtagaa ttggatatag agaaaagagt aaaattttta ggagaaagaa
      121 acgatattcc cgagttatta tctgtgatgg atgtttttgt aatgccttct ctttatgaag
      181 gtctgccaat ggttggatta gaagcacaag cagcaaaatt aaaattagtc ttagcagata
      241 ctatttccaa agatacaaag ttaacaaata atgtggattt tgtatcatta gatgaaacac
      301 cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc taataaaata acacaagaac
      361 taaagagtta tgatgtttcg tacactactg aacaaatgga gagtatatat agtagagatc
      421 attaaaaatg ttcaagatat tttaggagtg attatttttg aaaatatgtt atttgatatt
      481 ggcatacaat gatattggca acttaaagag attggtcaat cgattgaacg aaaatgcagt
      541 ttttattatt catatagata aaaaaactga tatagaacca tttattgaag catttaaatc
      601 atatgataat gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt ggggtggttt
      661 ttccattgta caagcagaaa tcaatttagt aaagagagca ttaca
//
LOCUS       DAZUKA010000339          696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000339 DAZUKA010000000
VERSION     DAZUKA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.351"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG8PN_003104"
     CDS             complement(54..629)
                     /locus_tag="ACG8PN_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4863151.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZUKA010000340          694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000340 DAZUKA010000000
VERSION     DAZUKA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.352"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..304
                     /locus_tag="ACG8PN_003105"
     CDS             <1..304
                     /locus_tag="ACG8PN_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4863152.1"
                     /translation="VVEFFFGNTAINYYKLFSLDRTQLLLVDTYDKKQVVMINTKKKI
                     TRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFKRPNKDIVVIEQKNTKDSI"
BASE COUNT          244 a          106 c          119 g          225 t
ORIGIN      
        1 agtagtggaa ttcttttttg ggaatacggc tattaattat tacaaattat tttcgttaga
       61 tcgaacacaa ctgctattgg tagacacata tgataaaaag caagtcgtga tgatcaatac
      121 gaagaaaaaa atcacaaggc aagaaatcga ctatgcgatt catcacgttt taaaaatgac
      181 acgagaagat gtcaaagtcc atgtaggagt gaaacaggag ctagaacgtg ccggaataca
      241 attcaaacgt ccaaataaag atattgtagt gatagaacaa aaaaatacga aggattctat
      301 ttaataacga ctaataatca tctttatggt aacctgctcc atatctaatg tatacaataa
      361 aaaacaaggc gagactgatt gatgtctcgc catttttgtt tccattctat tgacagctgc
      421 tttcttactt tgtatctttt taaaaacgtg tgatattttt tccgcgagtt ttttcaacgc
      481 aaggtatatc ttgtcataat tcgcctttcc aaaaaaattc aatacataat aagttatatc
      541 cgtttctttg gggataagaa gcaaaaaagg aaagacgtat tattgcttca aaaacttttg
      601 ccattgatta tgttgtagag acatttggtt atatttgttc aatcatcggt atcttaacgg
      661 ctacgattgt agtaggaaaa tctacttatt ttat
//
LOCUS       DAZUKA010000341          678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000341 DAZUKA010000000
VERSION     DAZUKA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.353"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8PN_003106"
     CDS             complement(<1..446)
                     /locus_tag="ACG8PN_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863153.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          203 a          118 c           91 g          266 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatg
//
LOCUS       DAZUKA010000342          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000342 DAZUKA010000000
VERSION     DAZUKA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.354"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACG8PN_003107"
     CDS             complement(<1..448)
                     /locus_tag="ACG8PN_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFS4863154.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          198 a          134 c          100 g          230 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 ca
//
LOCUS       DAZUKA010000343          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000343 DAZUKA010000000
VERSION     DAZUKA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.355"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(260..625)
                     /locus_tag="ACG8PN_003108"
     CDS             complement(260..625)
                     /locus_tag="ACG8PN_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863155.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          249 a           89 c          119 g          205 t
ORIGIN      
        1 tttttatgga ggaattgttt taattagttt actttcttca acactttatt ctttgaatat
       61 tatagatgtt tccacctcaa attcaatatc tattattgga atcattattg ctgcaattat
      121 tacacaactg tttttagtgt ttggagagaa gaaaagaagt aaaaaataga aaaactagtg
      181 atagttttgg tgatataaga aaaacatata tgtataaaaa gaagaaagta gcttctcttt
      241 gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag gctaccgccc
      301 ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag atcagctggg
      361 tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa aataaatagg
      421 aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg ttcctttgaa
      481 aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt acggaatgat
      541 aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa agcaacggaa
      601 ggtttttcca gcaagttttt atcatctttc atcaccacct aacaagtgtt attattttgt
      661 ta
//
LOCUS       DAZUKA010000344          649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000344 DAZUKA010000000
VERSION     DAZUKA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.357"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..385)
                     /locus_tag="ACG8PN_003109"
     CDS             complement(<1..385)
                     /locus_tag="ACG8PN_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863156.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEK"
     gene            complement(402..>649)
                     /locus_tag="ACG8PN_003110"
     CDS             complement(402..>649)
                     /locus_tag="ACG8PN_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863157.1"
                     /translation="SETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSL
                     GLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          228 a          117 c           65 g          239 t
ORIGIN      
        1 acttttctga aaaagttaga cccacgtaat aatcttccca gctttctctt tcaattaact
       61 gatccaattt ttgataatcc ataaattcat catgtatcaa aaagaataaa taacttgata
      121 ttttagcatc tagcttaatc agattaaata atgtttgttt aaaattgcta ccattaatat
      181 gactgataga ttctctagct tcttcacata aactttttac tttattaatt actactttgt
      241 catattcatt taacacacta ttattaccat tacttagacg ctcagattct acgaaacaac
      301 tcaaatcaga aatttcccaa aaaaattttt tgttaaaatc caatttcctc aactcctcca
      361 aactacgtaa atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa
      421 ttgtccacta aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact
      481 ctcgaaaagt gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg
      541 tttgatttca acattatttt agccgtagaa atttttcgct tagagatata attataaatc
      601 gtaatatgca tttcattctt aaagctttct cttaatttag tttcactta
//
LOCUS       DAZUKA010000345          625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000345 DAZUKA010000000
VERSION     DAZUKA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.359"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACG8PN_003111"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACG8PN_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAZUKA010000346          618 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000346 DAZUKA010000000
VERSION     DAZUKA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.360"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACG8PN_003112"
     CDS             <1..231
                     /locus_tag="ACG8PN_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4863158.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            390..>618
                     /locus_tag="ACG8PN_003113"
     CDS             390..>618
                     /locus_tag="ACG8PN_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4863159.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT          213 a          112 c          102 g          191 t
ORIGIN      
        1 ttaaaaaatc ttcctgaaac acttgatgca caacttcgca cgaaacttca aaacctttta
       61 acgtatgaag aaggtattta caatgctatg atttacccgt attctaatgg caaaatagag
      121 gccaagattc cccatatcaa gacactgaaa cgattatctt atggatttaa atcatttgag
      181 aacatgaaaa tcaggatttt tttgatcaat caattgattc aagtgaaata acaaaaaaat
      241 ctgagacaga aggtcagtcc ttctatcggc tctatacttt ttgatgttgg tgaacttcac
      301 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
      361 ttggctacca attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc
      421 taaatattct agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca
      481 aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc
      541 attgtctttg tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt
      601 tactgaatga tgtgtctg
//
LOCUS       DAZUKA010000347          609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000347 DAZUKA010000000
VERSION     DAZUKA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.361"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..509)
                     /locus_tag="ACG8PN_003114"
     CDS             complement(<1..509)
                     /locus_tag="ACG8PN_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4863160.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELF"
BASE COUNT          205 a          127 c           77 g          200 t
ORIGIN      
        1 ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct
       61 gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta
      121 aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca
      181 atatgatttg tataatttct gcttgcacac atatgataag atactgctgc tacaactaat
      241 aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc
      301 aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta
      361 ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc
      421 aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat
      481 acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga
      541 ttactttttc cacccaaata atacatcact tatcaaaaaa agtaaatact tttaattaaa
      601 tatatatat
//
LOCUS       DAZUKA010000348          599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000348 DAZUKA010000000
VERSION     DAZUKA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.362"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..599
                     /locus_tag="ACG8PN_003115"
                     /pseudo
     CDS             1..599
                     /locus_tag="ACG8PN_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          218 a          121 c          124 g          136 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca gacctactat tatcaagcga aaccgatcga
      361 aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc gaaaggctta
      421 cggtacccga aaattgaaga agtgtttagc aaagcgtggg cttcaactca gtcggcgccg
      481 aatcggtcga atcatgaaac gccgcggatt gacatctacc tatacgatcg ctcattttaa
      541 agggcaacga acagcttgta atgaagcgaa aacagcgaat gtattagatc ggaccttta
//
LOCUS       DAZUKA010000349          571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000349 DAZUKA010000000
VERSION     DAZUKA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.363"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>571)
                     /locus_tag="ACG8PN_003116"
     CDS             complement(<1..>571)
                     /locus_tag="ACG8PN_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863161.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQL"
BASE COUNT          190 a           92 c           68 g          221 t
ORIGIN      
        1 tacaattgta tttttttgat tttatcatct gcacaaagga gattttttaa atctaatcct
       61 tcttttattt caaaagttga attttttaaa tctataagag taaaaccaat tccttcatac
      121 tgaatttctg aaaatcgaat ttttctccca gttgatagat caaggggcag aaaacctttt
      181 attacattgt gagtgtcacc atcatttaca atgtcattag gaattttgat tttagaattc
      241 tgtggagcac ctattggaag ataataattc attataattt tgtagtcttc atgaggaaat
      301 aaagtacttt taggattgct aagatattct cgtaccgaat ctaagaatgg cttatccatt
      361 gaatcttcat ccccatttaa actaaaaaac attcctacaa ttgcttttgc aactctaagt
      421 gaattcattt tctttattga atgtgttaaa tcagacttac cattctttaa acttgtattg
      481 ttataaagga aatttttatt gtcttctgca aatctaatat attcctcaac atactcacta
      541 ccaaaaaaac tattacattt actacataac g
//
LOCUS       DAZUKA010000350          552 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000350 DAZUKA010000000
VERSION     DAZUKA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.365"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..451)
                     /locus_tag="ACG8PN_003117"
     CDS             complement(<1..451)
                     /locus_tag="ACG8PN_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4863162.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          157 a          100 c           68 g          227 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatttatt gttctactaa attcatcttt gttttacaag aacattatat ttctgttagt
      541 ttgttttgtc aa
//
LOCUS       DAZUKA010000351          548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000351 DAZUKA010000000
VERSION     DAZUKA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.366"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>548)
                     /locus_tag="ACG8PN_003118"
     CDS             complement(<1..>548)
                     /locus_tag="ACG8PN_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863163.1"
                     /translation="TNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFS
                     TKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKE
                     SIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVAS
                     INGKLVQTKLSKFGTTELTIPPG"
BASE COUNT          185 a           74 c           95 g          194 t
ORIGIN      
        1 cctggaggaa tagttaattc agttgtgcca aacttagata attttgtttg tactagtttg
       61 ccattaatag aagccacttg ccctttataa cgataaatag gtgtcgtcaa gacagtattt
      121 ttattattcg aattatctaa ttcaattgta agtttgttac tcataaaatg agttgttgga
      181 tttatttcct ggttacctag taaatattga tcattattaa tcatatcagg atgatagatt
      241 gactctttgt ttgcataatc agtatgattg taattttcag ctatagcaac agcgtcttca
      301 ggtgctataa gtgttaatga atctttgtta gcatggatag aattgcttac tcctgagaga
      361 tgcaatatta gtgcaagaaa cgtaaagacc attattttcc atgaattatt tttagtagaa
      421 aaaatataat agatcactat actcatcgta taggctataa ataaagaaga aaaaccattt
      481 aaccgccaga caaattgtag actcttaact ggtgtatggt taaaaagttg ccaaggaaat
      541 aaattggt
//
LOCUS       DAZUKA010000352          534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000352 DAZUKA010000000
VERSION     DAZUKA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.368"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG8PN_003119"
     CDS             <1..>534
                     /locus_tag="ACG8PN_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4863164.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZUKA010000353          533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000353 DAZUKA010000000
VERSION     DAZUKA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.369"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>533)
                     /locus_tag="ACG8PN_003120"
     CDS             complement(<1..>533)
                     /locus_tag="ACG8PN_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863165.1"
                     /translation="YISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYE
                     IYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKA
                     TVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFL
                     FLGLIGAFIFIKKLTAD"
BASE COUNT          212 a           91 c           86 g          144 t
ORIGIN      
        1 tcatcagcag ttaatttttt tataaaaata aaagcaccaa tcaatccgag aaataagaat
       61 aatccaatag tatagttgtt atagttatta tttaaaatat gtgtcagcat atcactagga
      121 gccataccac ttaattcttt tcccagtggg actttcaatt cttgtgctct agattgttga
      181 ataaaaggaa ttaagaatcc cgcacttaaa aaaaaggtga ggacggtagc ttttaaaaga
      241 cttaagagtc tagcgatttt ttggtcccaa aaataaaaag aaagaaacaa cgttcctcca
      301 ataaatacag aaaccatagc gacagaaagt aaatgagtat acactactag cgtcatgcca
      361 atagacaacc agtaccattt ttggtaatct ctaatataaa tttcgtaaca ccccattagt
      421 atcaaaggca aaaatgttaa agcgacagct tctcccaatg aagcacgatg gaatatgtca
      481 atggcacgat aggcggaaaa tgtataaata atcgaaaata acagcgaaat ata
//
LOCUS       DAZUKA010000354          521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000354 DAZUKA010000000
VERSION     DAZUKA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.370"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>521
                     /locus_tag="ACG8PN_003121"
     CDS             3..>521
                     /locus_tag="ACG8PN_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4863166.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSG"
BASE COUNT          198 a           67 c          108 g          148 t
ORIGIN      
        1 aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt ggaagtgaaa
       61 ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt agaggtgaag
      121 aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga tttttaattg
      181 gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat attatttttc
      241 atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa
      301 caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat gtaaaacatt
      361 ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt gcgacaaagt
      421 tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca gaaacaatat
      481 tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg a
//
LOCUS       DAZUKA010000355          510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000355 DAZUKA010000000
VERSION     DAZUKA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.372"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8PN_003122"
     CDS             complement(<1..434)
                     /locus_tag="ACG8PN_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863167.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DAZUKA010000356          503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000356 DAZUKA010000000
VERSION     DAZUKA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.375"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>503
                     /locus_tag="ACG8PN_003123"
     CDS             <1..>503
                     /locus_tag="ACG8PN_003123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863168.1"
                     /translation="AINELEKRNLEIHELNQKNNSILGMKIRNKINQLNQMKKRDNLL
                     PAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDSIKEP
                     DFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPNELFTDYDFT
                     LYVDGNVT"
BASE COUNT          207 a           63 c           80 g          153 t
ORIGIN      
        1 gctatcaatg aattggaaaa aagaaatctt gaaatacatg aattaaatca aaaaaataat
       61 tctattttag gcatgaaaat acgaaataaa attaatcaac ttaaccaaat gaaaaaacga
      121 gataatctgt tgccagcaaa atataccctt aaatcgatga ccggtaaggg gcctattaaa
      181 gactattcaa acagagagaa tcgcagtgat gaactaagaa aaaaagagtt aactaatcaa
      241 ggaacaccta aaaggattgc tgtgtatagt tgtatcactg gtaattatga ttctattaaa
      301 gaaccggatt tttctcatcc attggtagat tactatttgt atacagataa tgtgattaca
      361 agtgagcatt ggaatattag agagatacct aaatatatat cggaattaaa aaataatatt
      421 ttgatcaata gatatattaa atttcatcct aatgagctat ttactgacta cgattttact
      481 ttgtatgttg atggtaatgt aac
//
LOCUS       DAZUKA010000357          499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000357 DAZUKA010000000
VERSION     DAZUKA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.376"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>499)
                     /locus_tag="ACG8PN_003124"
     CDS             complement(<1..>499)
                     /locus_tag="ACG8PN_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4863169.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFL"
BASE COUNT          144 a          101 c          105 g          149 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaattt
//
LOCUS       DAZUKA010000358          490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000358 DAZUKA010000000
VERSION     DAZUKA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.378"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>490
                     /locus_tag="ACG8PN_003125"
     CDS             <1..>490
                     /locus_tag="ACG8PN_003125"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4863170.1"
                     /translation="MIEDAVITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAY
                     SFITNPDYKKSLVLVPILVIMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVG
                     TYLLGYSFGVIGVSFATLLGTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFV
                     CI"
BASE COUNT          141 a           71 c           87 g          191 t
ORIGIN      
        1 ctatgataga ggatgcggtt atcacgtctg agaatgatga tttcatcgaa agattcgttg
       61 agaaaaatac ctatattttt aaaatgtaca ttaggttagc gactctttct attcctatca
      121 tttgctgtgc atactctttt ataaccaacc ctgattataa aaaatcatta gttctagttc
      181 caatattagt tattatggca tgtttcagtg ggatgacaac aaatatcgga aatatatttg
      241 cagtatacaa taaaacaaat cagttattaa tgatttcagt agtgtcaggt ctattgagtg
      301 ttgttggcac ctatttatta ggctattctt ttggagtaat tggtgtctca tttgctactt
      361 tgctcggtac tttagtatta tttggactta gatattattt tggaaggaaa attgcttatt
      421 atgcatttga ttggaagcga atatcgctac ttttagttct ctattccgca atcgcttttg
      481 tatgcattca
//
LOCUS       DAZUKA010000359          489 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000359 DAZUKA010000000
VERSION     DAZUKA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.379"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>489
                     /locus_tag="ACG8PN_003126"
     CDS             <1..>489
                     /locus_tag="ACG8PN_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4863171.1"
                     /translation="ENQIICHPIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCI
                     LTKSMLHRVKFVNCKLTGTKLMESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSS
                     ADFVDCSLTKIMFSTNDLENASFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQA
                     LGF"
BASE COUNT          167 a           73 c           80 g          169 t
ORIGIN      
        1 gaaaaccaaa taatctgcca tccaattatt tacaaatcta ttttcaagta tgcatttttt
       61 gcttccaatg attttcaaag aatggagatt accgactgta tattcgaaaa ctgtgatttt
      121 tcaggctgta tattgacaaa aagcatgttg catcgcgtga aatttgtcaa ttgtaaatta
      181 acaggtacta aattgatgga aagttatatt gggaacacgt tattcgagaa ttgtaaaatg
      241 gactatgtaa atttatctgg ttcaaacata aaagaaagta cctttgagca ctctgtctta
      301 tcaagcgcag attttgtaga ctgttccctc actaaaataa tgttttctac taatgattta
      361 gaaaatgcta gcttttttga tactgattta aaaaatatcg atttaagtag caactccttt
      421 gaaatgattg aagtacgctc ggaacatcta aaaaattgta cgcttaatca gttgcaagca
      481 cttggtttt
//
LOCUS       DAZUKA010000360          482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000360 DAZUKA010000000
VERSION     DAZUKA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.380"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..296
                     /locus_tag="ACG8PN_003127"
     CDS             102..296
                     /locus_tag="ACG8PN_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863172.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          205 a           61 c           82 g          134 t
ORIGIN      
        1 aattgattct ttttatttat cataaagata tagatacata atcattttca cttacatata
       61 tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat gtagaagtaa
      121 aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa tatcctttac
      181 ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa ataaagaacg
      241 agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa aagtaaatcc
      301 aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa gattatagaa
      361 ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg
      421 attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa
      481 at
//
LOCUS       DAZUKA010000361          482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000361 DAZUKA010000000
VERSION     DAZUKA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.381"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..>482
                     /locus_tag="ACG8PN_003128"
     CDS             183..>482
                     /locus_tag="ACG8PN_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4863173.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSV"
BASE COUNT          178 a           75 c           71 g          158 t
ORIGIN      
        1 tattttcaat gaaatttgat tggaaaaaat aaaatgtgtt ttactgacat tgagttatgt
       61 tataaattta cgatacattt cttattgtaa cgtatcgaaa acaggtctat acttattcaa
      121 tggaatcgta ccagttccaa gtttttaagc tcgtaccaag cttagaaaga agaggcaatc
      181 gtatgaaaat aacagaaaag aatgtaaacc cgaaatttga aactaccgaa agcaatctaa
      241 aaaagtttga tttaactgtt actttatcga atcaacttgt agatagcatt gttgaaatgg
      301 atgctatgcc acaaaaaatt tatctttacg ctataggaca tcttgaccct aaaaatccgc
      361 cttttaataa taccgtcaaa gtatctaaaa aggctttatt tgcgttgatg tcttctagtt
      421 catcttcttc aaaacattca agattaaaac aagttattga aaaattgcat cagcaatctg
      481 tc
//
LOCUS       DAZUKA010000362          471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000362 DAZUKA010000000
VERSION     DAZUKA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.382"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(181..399)
                     /locus_tag="ACG8PN_003129"
     CDS             complement(181..399)
                     /locus_tag="ACG8PN_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863174.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          164 a           90 c           74 g          143 t
ORIGIN      
        1 aatggaaaca agcaaagacc agtagaagtt taaaaaatca ctttttccgc aatttgactt
       61 aataccgaaa aactggcttt agaacaccgt ttattcgaaa aaaaggatgt gaccgatttt
      121 tgtcacaccc ccgtaaaagt ttaacttctt gtcttttgaa taattagcag tcaatcctta
      181 ttatgattgc ctggatctgt agtagggctc atgatagaaa tgatcttcat gaattctgcg
      241 ccgctcagat tgacggtact tcttttcatc ccacaacatg aaccacaaag taaataacgg
      301 aacaaacaca agtaaagctg gacgtatgcc gaaattacca ataataatac ctaaaaacaa
      361 aacagttaat actaagccaa gacgtctcat tgatttcatg aattctcacc tctttaattt
      421 atgcgaacaa ttgttcgtat atttagtata cgaatatttg ttcggtttgt a
//
LOCUS       DAZUKA010000363          468 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000363 DAZUKA010000000
VERSION     DAZUKA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.384"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /locus_tag="ACG8PN_003130"
     CDS             <1..318
                     /locus_tag="ACG8PN_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4863175.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          187 a           70 c           61 g          150 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca cataattatt
      361 gacacaacct attctggaaa cttacaaata aaagcttatt cttcagccac cttcgggtgg
      421 ttatttttta taaaaaaatg taaaaaaata ttgatacata aataaatt
//
LOCUS       DAZUKA010000364          467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000364 DAZUKA010000000
VERSION     DAZUKA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.385"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..>467
                     /locus_tag="ACG8PN_003131"
     CDS             290..>467
                     /locus_tag="ACG8PN_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4863176.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPI"
BASE COUNT          173 a           76 c           67 g          151 t
ORIGIN      
        1 atccaaaagg aggttttaga aaagatttat tatacgtaaa aagctcaaga cattttctta
       61 gccataagga catgtttgat atttaagaag atatatgaat tttcttttct aagtataaag
      121 ataactatcc attatcaact aaaaaaacat ttttgaaaat aaacatcagc agtcctatca
      181 atgcatgtga gcttatagaa gttattcgca gcgcagcata attgcattag cttcttttcg
      241 tgttttttgc ctcttttccc tttaaatctt aatggaggag gtgtgcaata tgtcagataa
      301 cgacgaacgc gtaaatgccc agttgattcg attctttgaa aaggtcattt tgaacaccgc
      361 taatacttat tataaaaaac aagaagaaat tcaaaaacat gaacaacttg atcaactccc
      421 accatatttg tttactaaag atactataaa atttaaacaa ccgatta
//
LOCUS       DAZUKA010000365          464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000365 DAZUKA010000000
VERSION     DAZUKA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.386"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..380
                     /locus_tag="ACG8PN_003132"
     CDS             45..380
                     /locus_tag="ACG8PN_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863177.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          173 a           58 c           87 g          146 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtg
//
LOCUS       DAZUKA010000366          452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000366 DAZUKA010000000
VERSION     DAZUKA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.388"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          147 a           88 c           51 g          166 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aa
//
LOCUS       DAZUKA010000367          449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000367 DAZUKA010000000
VERSION     DAZUKA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.390"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>449
                     /locus_tag="ACG8PN_003133"
     CDS             <1..>449
                     /locus_tag="ACG8PN_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4863178.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           95 t
ORIGIN      
        1 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
       61 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
      121 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
      181 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
      241 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
      301 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
      361 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
      421 gccgataaat atgagccaac agtagaagg
//
LOCUS       DAZUKA010000368          419 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000368 DAZUKA010000000
VERSION     DAZUKA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.392"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>419
                     /locus_tag="ACG8PN_003134"
     CDS             <1..>419
                     /locus_tag="ACG8PN_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4863179.1"
                     /translation="DDMNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQIN
                     HNLESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIE
                     KTNLENQLSGKNAELQGKQNEINAKIDEINQKIAEIN"
BASE COUNT          190 a           39 c           79 g          111 t
ORIGIN      
        1 tgatgatatg aataagaaaa ttgtagtaag tggattaatt ataggtggat tagtgttagc
       61 agggatcgca gggaaaaact ttgccgatga cattttgtta tggggtggag aagataatat
      121 tgaacaaatc aatcataatt tggagtcatt agataaagca ctagaaaata aagagcaaaa
      181 tattagtagt ttaaattcac gattatcttc aaataatcaa gaacttgagc aagcaaaggc
      241 taacgttgaa gaatataaac aaaaagtgtt gactttggaa aatgataaaa atcaattaac
      301 tatagagaaa acaaatttag agaatcaatt atcaggaaaa aatgctgaat tgcaaggtaa
      361 acaaaatgaa atcaatgcta aaattgatga aataaatcaa aaaatagctg aaataaatc
//
LOCUS       DAZUKA010000369          407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000369 DAZUKA010000000
VERSION     DAZUKA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.393"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..331
                     /locus_tag="ACG8PN_003135"
     CDS             <1..331
                     /locus_tag="ACG8PN_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4863180.1"
                     /translation="LGNKTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHK
                     GICTAAFRANNLSCMIYGIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNL
                     EHLVLQH"
BASE COUNT          156 a           62 c           60 g          129 t
ORIGIN      
        1 tttagggaat aaaacattag gacaaacgat acacaataaa agatgttatg tgatattaat
       61 taatcaaaac ctatcacctt ctatgaaaag atttgtatta tggcatgaaa tagcccacgt
      121 tagactccat aaaggtattt gcacagcagc ttttagagca aacaacctta gttgtatgat
      181 ttatggtata gaagctgaag ctaatatgtt tgcaattgaa atgttaacta gaacaataga
      241 tgaacaagat ttatctgata tgagtcagtt ccaaattatt gaatatttag ggcttcctca
      301 taatcttgaa catttagtat tacaacatta aaaataaaaa agcccatgct ttcacatgga
      361 ctctttcctc atttctgagt aaactctaca ataattatat caaagaa
//
LOCUS       DAZUKA010000370          405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000370 DAZUKA010000000
VERSION     DAZUKA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.394"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..262)
                     /locus_tag="ACG8PN_003136"
     CDS             complement(62..262)
                     /locus_tag="ACG8PN_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4863181.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          124 a           75 c           59 g          147 t
ORIGIN      
        1 aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa gtcaattttt
       61 attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca acatcgtaag
      121 acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct gaaaaatgtg
      181 caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct gcgttgaacc
      241 attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat gtacgttttt
      301 tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata tatcgctcaa
      361 attacgtttc aaaatagatt actcatttaa tatatcatgc ttttt
//
LOCUS       DAZUKA010000371          402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000371 DAZUKA010000000
VERSION     DAZUKA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.395"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..300
                     /locus_tag="ACG8PN_003137"
     CDS             <1..300
                     /locus_tag="ACG8PN_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4863182.1"
                     /translation="TPSGDKIQKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQ
                     AEKLLEGLNSDAETNYYGEPLDDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
BASE COUNT          163 a           55 c           65 g          119 t
ORIGIN      
        1 actccatctg gagataaaat acaaaaagtt gcagattact ttaatgtatc aactgattat
       61 cttttaggac gtactgataa acgccattat tatgatttaa cagaaaaaga taaacaagat
      121 attgctttac aagctgaaaa attacttgaa ggcttgaatt cagatgctga aacaaattat
      181 tatggtgaac ctttagacga tgaagataag gaaaaattat acgatgccat tcaacttgca
      241 cttgccttaa ccaaagttaa agcaaagaaa aaattcactc ctaaaaaata tcgtcaatag
      301 gtgggctttg gtatgcattt aaacaaatac tggatattag aagaaataga aaaattattt
      361 gtagagtttg gatctgatgc gattaatcca tataaaataa tc
//
LOCUS       DAZUKA010000372          387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000372 DAZUKA010000000
VERSION     DAZUKA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.396"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..>387
                     /locus_tag="ACG8PN_003138"
     CDS             108..>387
                     /locus_tag="ACG8PN_003138"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4863183.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDS"
BASE COUNT          146 a           41 c           74 g          126 t
ORIGIN      
        1 cttaaaatga tgtttgtact tacttaaatt aggtataatt agtatatgtt ttgtgagaaa
       61 tttagactta caataagcag tgaatttata atcaacgtat aggagacatg gatgaaatga
      121 aactaattgg tatagattta gacggtactc ttttgaattc tgaccagagc attagtcaaa
      181 aaaatgcaag aacaatgaaa catttaccgg aagactgttt tccattcatt tgttctggaa
      241 gagaagtaga agacattaat aatattctca aaaaatctgg tttaaggata ccagcagtag
      301 ggttaaatgg ttctgttgga tttgatggag aaaaaaagat atttgagtct tccttcgata
      361 aaaatgatgt aaaaaaaatt gattctg
//
LOCUS       DAZUKA010000373          375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000373 DAZUKA010000000
VERSION     DAZUKA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.398"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8PN_003139"
     CDS             complement(<1..132)
                     /locus_tag="ACG8PN_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4863184.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAZUKA010000374          373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000374 DAZUKA010000000
VERSION     DAZUKA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.400"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          132 a           60 c           61 g          120 t
ORIGIN      
        1 cataccatat ttttgaaaaa aagtgagaaa aaaggcttat aattggagtg gtagttaatt
       61 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
      121 acaatcttaa tactaactta aaaaatatct cctttcacaa gtatggtgat aaaattcgtt
      181 ccgggctacc tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      241 atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcatgaa
      301 tcggctacac tagactagac agaaaaaata aggtgtgtag aataataaaa aacacactgg
      361 aggatttttc atg
//
LOCUS       DAZUKA010000375          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000375 DAZUKA010000000
VERSION     DAZUKA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.402"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          139 a           53 c           76 g           91 t
ORIGIN      
        1 aatagtgata attagtaata tacgaattat aagatacatt ttatatttaa tgtatgtaaa
       61 taaataaaat gactgacgga tattgaatag taataaataa caataattca gacggataca
      121 gtgagtgaag tagcttgcta cgaacgaaca ggcagaatga aacggtagtt tcattagagc
      181 aagtcgcttg cgacgtagcg ttaccactga tgtaactggc aagttacata gagagagttt
      241 gcttgcaaac gaacgttacc cacttgaata gaaaacaaaa ataacatatt agtgaatttt
      301 gaataggagc cacaactcaa ccaaccgctt gcggtgggtg agtcccacgg aggaaaaga
//
LOCUS       DAZUKA010000376          345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000376 DAZUKA010000000
VERSION     DAZUKA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.403"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           65 c           56 g          111 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttct
//
LOCUS       DAZUKA010000377          339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000377 DAZUKA010000000
VERSION     DAZUKA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.405"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..>339
                     /locus_tag="ACG8PN_003140"
     CDS             15..>339
                     /locus_tag="ACG8PN_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863185.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYS"
BASE COUNT          137 a           59 c           59 g           84 t
ORIGIN      
        1 aaaggagaaa aaagatgaag ccaaatcctt cagaagcagg aaaacgaatc aaacaattac
       61 gcctttcatg tggttttact atggaagaac ttggaaggaa aatagataat tcaccacgtg
      121 cgaccatatc caactgggaa cgtgggacca atttaccaaa tccacaaaag ctgaaattat
      181 tatcaactat cactaattca acaattgact ggattaaatg gggaacttta gaagaatata
      241 taacttcata ccttatcgat atagggtatg agctatatat taaggacttc ccagaaatac
      301 ctcacaaagt ctttaaagac attcaagaaa gatatagtg
//
LOCUS       DAZUKA010000378          324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000378 DAZUKA010000000
VERSION     DAZUKA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.409"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>324
                     /locus_tag="ACG8PN_003141"
     CDS             <1..>324
                     /locus_tag="ACG8PN_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4863186.1"
                     /translation="VRTLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEV
                     LSYAIAPTESTYVWNELLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCV
                     HVSRN"
BASE COUNT          102 a           62 c           62 g           98 t
ORIGIN      
        1 agttagaact ctaaatgata agtatgtcgc tattttcatg gatgctactt atattccatt
       61 aaaacggcaa accgtctcca aagaagcaat ctatattgcc attggtatac gagaagacgg
      121 cactaaagaa gtactaagtt atgcgattgc tccgactgaa tcgacctacg tttggaatga
      181 gttgttacag gacatttact ccagaggggt tcatgatgtc ttactgttta ttaccgatgg
      241 cttaaaaggc atgaaagata cgattcacca gatttatcct aaagctaaat accaacattg
      301 ctgtgttcac gtttcccgta atat
//
LOCUS       DAZUKA010000379          323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000379 DAZUKA010000000
VERSION     DAZUKA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.410"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8PN_003142"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8PN_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4863187.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZUKA010000380          300 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000380 DAZUKA010000000
VERSION     DAZUKA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.413"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>300
                     /locus_tag="ACG8PN_003143"
     CDS             <1..>300
                     /locus_tag="ACG8PN_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4863188.1"
                     /translation="TEKKRKVSVTLSSNSRQAWNQANLLLNEKIAEKIKRNEELPLTF
                     GELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDDVLVKNVNSNLIQDMLDYY"
BASE COUNT          133 a           43 c           42 g           82 t
ORIGIN      
        1 taactgaaaa aaagagaaaa gtgtccgtta ctttatctag caattcacgc caagcttgga
       61 atcaagctaa cttacttttg aatgaaaaaa tcgcagaaaa aataaaacga aatgaagaat
      121 taccactaac ttttggagaa ttaaaaacta aatgggatga aaaatacaag ccaactgtaa
      181 aagaaagttc ctatcgaaca actcaggttt atctatcatt aatttcaaaa tatataaaag
      241 atgatgtact tgtaaaaaac gttaatagta atcttataca agatatgcta gattactact
//
LOCUS       DAZUKA010000381          299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000381 DAZUKA010000000
VERSION     DAZUKA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.414"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..>299
                     /locus_tag="ACG8PN_003144"
     CDS             36..>299
                     /locus_tag="ACG8PN_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFS4863189.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHE"
BASE COUNT          120 a           39 c           48 g           92 t
ORIGIN      
        1 actattttta cttaatttat gaaaggttgt ttaccatgtt agaccaaaca gatataaata
       61 tcattgagga actatcaaaa aatagtcgaa tcacaatgaa agaacttgga gaaaaagtac
      121 atttgactgg acccgctgtc tcggcgagag tgacaaaact agaggaaagt ggagttattg
      181 aaaattatac agtaaaatta aatatggaaa agctagggtt ttcgattcac gcttttttaa
      241 ttataattac gcaaaattta aatcataaac cctatttatc atttattaaa aatcatgaa
//
LOCUS       DAZUKA010000382          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000382 DAZUKA010000000
VERSION     DAZUKA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.416"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG8PN_003145"
     CDS             <1..>282
                     /locus_tag="ACG8PN_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4863190.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZUKA010000383          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000383 DAZUKA010000000
VERSION     DAZUKA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.417"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>282)
                     /locus_tag="ACG8PN_003146"
     CDS             complement(<1..>282)
                     /locus_tag="ACG8PN_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4863191.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT           90 a           55 c           48 g           89 t
ORIGIN      
        1 ctcgtagcca agtgtaccgt gcaaccgaag gtggttccac caattgacat agtcaaataa
       61 ctccaaatcc aattgttgta aggtttcaaa tgtgtattga tagacaaatt ctactttcaa
      121 cgacttataa gttgattcag ctacggcatt atcaaaagga cagcctttat gactcaatga
      181 tcgattgatg tcaaaagttg ttaataattc atcaatagct tggttatcaa actcttttcc
      241 acgatcagta tgaaaaatct caacctctgt cagaggttgt tt
//
LOCUS       DAZUKA010000384          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000384 DAZUKA010000000
VERSION     DAZUKA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.418"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..279)
                     /locus_tag="ACG8PN_003147"
     CDS             complement(<1..279)
                     /locus_tag="ACG8PN_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863192.1"
                     /translation="MIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDN
                     VWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYF"
BASE COUNT          117 a           36 c           43 g           85 t
ORIGIN      
        1 aaaatagtag atattataac ttaaaacaag atttcccatc attttataaa ataaaaaagc
       61 tggataaaga gtaagccatg gataaaaaat attcatcatt gttccatgat gatcgaagtt
      121 attgaagttt actggagaac tccatacatt atctagtcct atgattcgag ataaatgaaa
      181 gtaagtatcc tgattgacac tatgatagat tcccttagac atgaatagag ggatgacgta
      241 agcaaaactt agaaataaca taaaaatgat atctattatt t
//
LOCUS       DAZUKA010000385          253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000385 DAZUKA010000000
VERSION     DAZUKA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.423"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG8PN_003148"
     CDS             <1..>253
                     /locus_tag="ACG8PN_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4863193.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZUKA010000386          238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000386 DAZUKA010000000
VERSION     DAZUKA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.432"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>238)
                     /locus_tag="ACG8PN_003149"
     CDS             complement(<1..>238)
                     /locus_tag="ACG8PN_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863194.1"
                     /translation="WGIFLLFNMYMPTMRADDVVYASRLDELGYLGASIEHYKTWSSR
                     IIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT           93 a           46 c           27 g           72 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaa
//
LOCUS       DAZUKA010000387          233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000387 DAZUKA010000000
VERSION     DAZUKA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.433"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZUKA010000388          231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000388 DAZUKA010000000
VERSION     DAZUKA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.435"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..201
                     /locus_tag="ACG8PN_003150"
     CDS             <1..201
                     /locus_tag="ACG8PN_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4863195.1"
                     /translation="YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTR
                     KEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT           77 a           45 c           45 g           64 t
ORIGIN      
        1 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
       61 cgaagtttac gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat
      121 aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta
      181 ttattgggaa tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAZUKA010000389          228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000389 DAZUKA010000000
VERSION     DAZUKA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.436"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>228
                     /locus_tag="ACG8PN_003151"
     CDS             <1..>228
                     /locus_tag="ACG8PN_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4863196.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           82 a           42 c           41 g           63 t
ORIGIN      
        1 cttgaaacca gaaggccgta aggcagttga tattgaaaat tcgattaatg aatggcttca
       61 atccgtaccc aaaaatcttt tcaaatcaat tacctttgat tgtggaaaag aattctctaa
      121 ttggaaaagt attagtaata cgaatgatat tgatatttat ttcgcagacc caggaacgcc
      181 ttcccaacgg ggattaaatg aacattcaaa cggactcctc cgaaaaga
//
LOCUS       DAZUKA010000390          227 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000390 DAZUKA010000000
VERSION     DAZUKA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.437"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACG8PN_003152"
     rRNA            complement(<1..>227)
                     /locus_tag="ACG8PN_003152"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAZUKA010000391          226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000391 DAZUKA010000000
VERSION     DAZUKA010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.438"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           99 a           29 c           42 g           56 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgct
//
LOCUS       DAZUKA010000392          225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000392 DAZUKA010000000
VERSION     DAZUKA010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.439"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8PN_003153"
     CDS             <1..156
                     /locus_tag="ACG8PN_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4863197.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT           95 a           16 c           53 g           61 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa cttta
//
LOCUS       DAZUKA010000393          224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000393 DAZUKA010000000
VERSION     DAZUKA010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.440"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8PN_003154"
     CDS             <1..>224
                     /locus_tag="ACG8PN_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4863198.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZUKA010000394          221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000394 DAZUKA010000000
VERSION     DAZUKA010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.441"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>221
                     /locus_tag="ACG8PN_003155"
     CDS             22..>221
                     /locus_tag="ACG8PN_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4863199.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCG"
BASE COUNT           81 a           41 c           43 g           56 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga g
//
LOCUS       DAZUKA010000395          220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000395 DAZUKA010000000
VERSION     DAZUKA010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.442"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG8PN_003156"
     rRNA            complement(<1..175)
                     /locus_tag="ACG8PN_003156"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZUKA010000396          212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000396 DAZUKA010000000
VERSION     DAZUKA010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.443"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>212
                     /locus_tag="ACG8PN_003157"
     CDS             82..>212
                     /locus_tag="ACG8PN_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4863200.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     "
BASE COUNT           77 a           36 c           37 g           62 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat ac
//
LOCUS       DAZUKA010000397          212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000397 DAZUKA010000000
VERSION     DAZUKA010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.444"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..157
                     /locus_tag="ACG8PN_003158"
     CDS             2..157
                     /locus_tag="ACG8PN_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4863201.1"
                     /translation="MYGGTTHSGKYYGNGVYCTKNKCTVDWAKATTCIAGMSIGGFLG
                     GAIPGKC"
BASE COUNT           75 a           24 c           49 g           64 t
ORIGIN      
        1 tatctatggg ggtaccactc atagtggaaa atattatgga aatggagtgt attgcactaa
       61 aaataaatgt acggtcgatt gggccaaggc aactacttgt attgcaggaa tgtctatagg
      121 tggtttttta ggtggagcaa ttccagggaa gtgctaaaat gaaaaaaaat gctaagcaaa
      181 ttgttcatga attatataat gatatatcta ta
//
LOCUS       DAZUKA010000398          208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000398 DAZUKA010000000
VERSION     DAZUKA010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.denovo.445"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>208
                     /locus_tag="ACG8PN_003159"
     CDS             <1..>208
                     /locus_tag="ACG8PN_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4863202.1"
                     /translation="ITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKE
                     IGKTKIEHLSPLISSVVQFIGLNI"
BASE COUNT           71 a           30 c           40 g           67 t
ORIGIN      
        1 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
       61 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      121 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      181 tcgttcaatt catcggctta aatatcta
//
LOCUS       DAZUKA010000399        13213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.080, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000399 DAZUKA010000000
VERSION     DAZUKA010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.080"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8PN_003160"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8PN_003160"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4863203.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG8PN_003161"
     CDS             complement(2159..3031)
                     /locus_tag="ACG8PN_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4863204.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG8PN_003162"
     CDS             complement(3373..4245)
                     /locus_tag="ACG8PN_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4863205.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG8PN_003163"
     CDS             4373..4942
                     /locus_tag="ACG8PN_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4863206.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG8PN_003164"
     CDS             5009..5746
                     /locus_tag="ACG8PN_003164"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4863207.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG8PN_003165"
     CDS             5977..7245
                     /locus_tag="ACG8PN_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4863208.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG8PN_003166"
     CDS             7732..8196
                     /locus_tag="ACG8PN_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4863209.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG8PN_003167"
     CDS             8180..8809
                     /locus_tag="ACG8PN_003167"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4863210.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8PN_003168"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8PN_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4863211.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG8PN_003169"
     CDS             10688..11134
                     /locus_tag="ACG8PN_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4863212.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG8PN_003170"
     CDS             complement(11333..11848)
                     /locus_tag="ACG8PN_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4863213.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG8PN_003171"
     CDS             11965..13167
                     /locus_tag="ACG8PN_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4863214.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZUKA010000400        12879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.084, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000400 DAZUKA010000000
VERSION     DAZUKA010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.084"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(371..607)
                     /locus_tag="ACG8PN_003172"
     CDS             complement(371..607)
                     /locus_tag="ACG8PN_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863215.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(790..1161)
                     /locus_tag="ACG8PN_003173"
     CDS             complement(790..1161)
                     /locus_tag="ACG8PN_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4863216.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1158..2486)
                     /locus_tag="ACG8PN_003174"
     CDS             complement(1158..2486)
                     /locus_tag="ACG8PN_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4863217.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8PN_003175"
     CDS             complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8PN_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4863218.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3034..3404)
                     /locus_tag="ACG8PN_003176"
                     /pseudo
     CDS             complement(3034..3404)
                     /locus_tag="ACG8PN_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3549..3734
                     /locus_tag="ACG8PN_003177"
     CDS             3549..3734
                     /locus_tag="ACG8PN_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4863219.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3892..4059
                     /locus_tag="ACG8PN_003178"
     CDS             3892..4059
                     /locus_tag="ACG8PN_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863220.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4677..5336
                     /gene="vanR-B"
                     /locus_tag="ACG8PN_003179"
     CDS             4677..5336
                     /gene="vanR-B"
                     /locus_tag="ACG8PN_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4863221.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5339..6700
                     /gene="vanS-B"
                     /locus_tag="ACG8PN_003180"
     CDS             5339..6700
                     /gene="vanS-B"
                     /locus_tag="ACG8PN_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4863222.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6791..8092)
                     /locus_tag="ACG8PN_003181"
     CDS             complement(6791..8092)
                     /locus_tag="ACG8PN_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4863223.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            8371..9177
                     /locus_tag="ACG8PN_003182"
     CDS             8371..9177
                     /locus_tag="ACG8PN_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4863224.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9195..9902
                     /locus_tag="ACG8PN_003183"
     CDS             9195..9902
                     /locus_tag="ACG8PN_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFS4863225.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            9899..10870
                     /gene="vanH"
                     /locus_tag="ACG8PN_003184"
     CDS             9899..10870
                     /gene="vanH"
                     /locus_tag="ACG8PN_003184"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFS4863226.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGARVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10863..11891
                     /gene="vanB"
                     /locus_tag="ACG8PN_003185"
     CDS             10863..11891
                     /gene="vanB"
                     /locus_tag="ACG8PN_003185"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4863227.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            11897..12505
                     /locus_tag="ACG8PN_003186"
     CDS             11897..12505
                     /locus_tag="ACG8PN_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4863228.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(12571..12744)
                     /locus_tag="ACG8PN_003187"
     CDS             complement(12571..12744)
                     /locus_tag="ACG8PN_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863229.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3411 a         2851 c         3222 g         3395 t
ORIGIN      
        1 ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca
       61 cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc
      121 gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga
      181 agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc
      241 cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg
      301 gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc
      361 ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg
      421 aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga
      481 tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg
      541 cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc
      601 cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg
      661 gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg
      721 gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata
      781 taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc
      841 ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt
      901 cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat
      961 ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag
     1021 cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt
     1081 gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta
     1141 attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa
     1201 atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg
     1261 tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg
     1321 tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac
     1381 aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat
     1441 tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata
     1501 tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg
     1561 atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa
     1621 ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc
     1681 gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct
     1741 ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag
     1801 gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata
     1861 aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa
     1921 tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc
     1981 cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc
     2041 acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc
     2101 tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga
     2161 ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc
     2221 acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg
     2281 gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc
     2341 agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc
     2401 ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg
     2461 ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt
     2521 cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc
     2581 cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat
     2641 agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca
     2701 ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc
     2761 gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac
     2821 aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca
     2881 ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac
     2941 tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc
     3001 agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt
     3061 cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc
     3121 ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat
     3181 ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac
     3241 cggcagactg ggaatggccc gtcgttttcg atgttggcaa gatagcgcca gtcaatattt
     3301 attttctctg ccattgcttt gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc
     3361 gcgccgaaag tttcaaagcc gggacaatct tcaatattcg ccatatcgca cctcccactt
     3421 acattgtata tttcatactc tgcccgtgga atgttggtat atgcaccttt cttaaagttt
     3481 atatttcaca ttttgtttct tttattcttc gagcaaaaga attataatgt accctgtgga
     3541 ggtgtactat ggactacatg acgttgaaag aggcaggcga gaaatggggt gtgcccccac
     3601 gatggataaa ctactattgt gctgcgggac gcatccccgg cgctgtaaag atggcgacga
     3661 tctggctcat tcccaaaact gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg
     3721 cggttactga atagtataat gaaaccggcg cattttggat atggttttga agagagagaa
     3781 agcatggtat tttgtcacgg aaaaactata agatgataga tggcaggctg ctccagaccg
     3841 ataaaaagta ttctcatctg aaacagaaag aaaagattta cacatggatg tatgaagaag
     3901 cgaaacttta tcatgacaga aatggaaaat gcccgaaaca aagacgagga agctgctgct
     3961 gtatcagccg tatatggtag aattgaaaag gccggcatct ggattcttta tggtgaagtc
     4021 tccagacatt ggataactgc tggtctaatg cgcctttgag tatggggtga ttgattttaa
     4081 taccggggag gtacggcttt ttgaggaaaa tattctggga tttacatttg gcgattatta
     4141 catttcctgt aactgcaagg acacggatga tgaagatatg aggaatatag acttgctgat
     4201 tttctactgt cttgaacggt attaggactg aatctgctgc gcggagtgcg gcaagagcga
     4261 ccggagggag tacacacagg ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc
     4321 aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc ccaacaagca aaacgagaac
     4381 gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag gatattctga tccattgctt
     4441 gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga aacatcccaa acattaagaa
     4501 ggtgttaatt attattatct tgatttaatt cgtttaaacc aataatatag atacatacac
     4561 catgaaaaga agcgagtatc ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg
     4621 aaactacagg gaaactacag actgttatgt tatcttctta aaatggaggg atttttatgt
     4681 cgatacgaat tctacttgtc gaggatgatg atcatatctg taatacagta agggcgtttc
     4741 tggctgaggc aggatatcag gtggatgcct gcacagatgg aaatgaggca tacaccaagt
     4801 tttacgaaaa cacttatcaa ctggttattc ttgatattat gctgcccggt atgaacgggc
     4861 atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc cattctgatg atgacagccc
     4921 tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa
     4981 agccattcaa gatgcagatt ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg
     5041 cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct gccggaggat ttcacggtac
     5101 tttgtgacgg tacagagctg cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg
     5161 tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt gtcacgtata tggggatatg
     5221 actttgaggg tgatggcagc acagtccaca ctcatatcaa aaatttgcgg gccaagctgc
     5281 cggaaaatat catcaaaacc attcgcggtg taggataccg attggaggaa tcataataat
     5341 ggaaagaaaa gggattttca ttaaggtttt ttcctatacg atcattgtcc tgctactgct
     5401 tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg tcttatttca gagtgatgga
     5461 attacagcaa acagtaaaat cctatcagcc attggtggaa ctgattcaga atagcgatag
     5521 gcttgatatt caagaggtgg cagggctgtt tcactacaat aaccaatcct ttgagtttta
     5581 tattgaagat aaagagggaa gcgtactcta tgccacacca aatgccaata catcaaatag
     5641 ttttagaccc aactttcttt atgtggtaca tagagatgat aatatttcga ttgttgctca
     5701 aagcaaggca ggtgtgggat tgctttatca agggctgaca attcggggaa ttgttatgat
     5761 tgcgataatg gttgtattca gccttttatg cgcgtatatc tttgcgcggc aaatgacaac
     5821 gccgatcaaa gccttagcgg acagtgcgaa taaaatggca aacctgaaag atgtaccgcc
     5881 gccgctggag cgaaaggatg agcttggcgc actggctcat gatatgcatt ccatgtatgt
     5941 caggctgaaa gaaaccatcg caaggctgga ggatgaaatc gcaagggaac atgagttgga
     6001 ggaaacacag cgatatttct ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc
     6061 tacaagcgtt ctgttggagg gaatgcttga aaatatcggt gactacaaag atcattctaa
     6121 gtatctgcgc gaatgcatca aaatgatgga taggcagggc aaaatcattt ccgaaatact
     6181 ggagcttgtc agcctgaatg atgggagaat cgtacccata gctgaaccgt tggacatagg
     6241 gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg
     6301 gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa
     6361 ggcactatcc aatgtcatat tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg
     6421 gatatggagt gagcctggtg ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca
     6481 cattgatgat actgcgcttc caaggctgtt caccccattc tatcgcattg atcaggcgcg
     6541 aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt
     6601 acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt
     6661 tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat
     6721 tttgggctct ttgtcaaata ggactgatag agtgagataa gaacatctaa ggtagaattc
     6781 cttttctact ttagatgttt tttgattcat aggtaatcag attatttagt aagaaaattc
     6841 tggtcttcat tttccgaaag cttcgaaagc cataagcgac tcgtttcaat gctttgatgt
     6901 gggtatgcag attttctatt ttggcatttg aataaggcaa ttcgagggct aaacgaatgc
     6961 cggattcata gtttataaaa gcttttttta tagaatgcag ttcttttgga acggaattgg
     7021 gcatcgttct tacaagttta aagaaagtgt ccgcctcttt gtcgtgaaac gcttgaagaa
     7081 tttcttgata gtaaaaatag gcgattcgta attcgtcaga gatcgaaagc agacgatcca
     7141 ccacttcact ttcggtgaga tacgcccaac caaaattgcg ccatttggtc catttgtcat
     7201 aaacaagctt gtcctgtcgt ttgatcagaa gccgagacaa cgcctttaat tgtcgataat
     7261 atttggcttg tgttggatcg tttcgatcaa agcttttcat gattcgtacg cgaatatcct
     7321 caaatttacg aatgagatgt ttgacaatgt gaaaacgatc cacaacgagt tttgcctttg
     7381 gaaaacattc ctttatcaga gaggcataag aaacattcat gtccgtcacg atgaccttta
     7441 ccttcaatcg tgcgcgtcgt tcaaattgca taaaataaga aacgagagcc tttttcttcc
     7501 gtgaaggaag aatatccagt aattttccag tatcgccatc catacaagaa aaggagtagg
     7561 gaccgacttg atttttcagt gaacgaaatt catccattaa taaacaacgc ggtaaccaat
     7621 ttctttttac ttttgtcatg gggatcagag aagcgagtgt ccgagaaacg gtcgaccaag
     7681 aaacgccgca atgcttagca attgtcgtca ttgcttgttt ttcagttagc tcttctaaaa
     7741 ttttatagcg cacttgttta gcgataaagc agtttctatc gacaatgtag gtattcgccg
     7801 taaaataggt gtggcagcca cgacagtggt agcgttgttt cgctaaagca agataagtca
     7861 gttggttcgc acatttatta agttgaatga gcgatttttt ctttccgttc ttaacgatca
     7921 tgccttcatg attcatctgt ttacacaaag gacagcgacg aatttcttta cttaacgtcg
     7981 cagaaacaac aagactcttg atattcttaa tgctcttcga ttccacattt gtgatgaaaa
     8041 tgtttttatc tttgattcca agtagtagtt tgatatgatg aagatcaggc atacagaacg
     8101 ctcctttgat tgtgttgtgg tgattcaatt ctaacggagc ttctgtatgt tttgtttttt
     8161 catgctcaaa agggaccgat agaatgattt tctatcagtc ctagaaatta tagagccgat
     8221 tttgaccgac aggtataacc ctgccggtct ttttttgctg ctacaggaaa actacagact
     8281 ggctacaggg aaagtacaga tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt
     8341 agttttattg caaaggaggc attcgatcaa atggaaaaaa gcaactatca ttccaatgcg
     8401 gatcatcaca aacggcatat gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc
     8461 attatttcgt tcacagtctg cacgctgttt ttggggtgga gactggcttc cgtattggag
     8521 gcaacacaga taccgcccat ccctgcaact catacaggca gcagcactga cgtagtggag
     8581 aatttggagg aaaacgctct tgccaccgcc aaagaacagg gagatgaaca ggaatggagc
     8641 ctgattttag tgaacaggca gaaccccatc cccgcacagt acgatgtgga gcttgagcaa
     8701 ctatcaaatg gtgagcggat agatattcgg atttctccct atcttcaaga tttgtttgat
     8761 gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg gataccgaac aacagaaaaa
     8821 cagcaagaaa ttatggatga aaaaattgcc gaatataagg cgaaaggcta cacctctgca
     8881 caggctaaag cggaagcaga aacttgggtg gccgtgccgg gaacgagcga gcatcagctt
     8941 ggtcttgctg tggatatcaa tgcggacgga attcattcaa caggcaacga ggtttataga
     9001 tggctggatg aaaacagcta tcgttttggt tttattcgtc gctacccacc agacaagaca
     9061 gagataaccg gtgtgagcaa cgagccgtgg cattaccgat atgttggcat cgaagctgcc
     9121 acagagatgt acaaccaagg ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa
     9181 aaggatgtta tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa
     9241 tgagacgggc gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct
     9301 atgcgcagac gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat
     9361 tatacaacca caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa
     9421 agctggcagc aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct
     9481 ggttgttcct gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt
     9541 ttccagagcc aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga
     9601 ttgatttaaa agtgcgaaac gataccgact gcacctacca aatatgggtg accttagatg
     9661 atgagaaaat catcggtcag gtgttcgccg acaaagagcc ccaagcatta tacaaaatta
     9721 caaatggcag tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta
     9781 aacggatgca agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa
     9841 acaaatgcaa aatctgttat cccctcccgg aaagtgtgga tattcaggag gagaaccaat
     9901 gagaaaaagt gtgggcatta ctgtttttgg atgcgaacag gatgaggcaa atgctttccg
     9961 cgctttatcg ccggattttc atattatccc tacgctgatt agcgatgcga tatcggcaga
    10021 caacgcaaaa ttggccgctg gcaatcaatg cgttagcgta agccataagt ccgaggtttc
    10081 cgaggcgaca attcttgcgc tgagaaaggt cggggtaaaa tacatttcta cccgcagcat
    10141 cggctgcgat cacattgata cgactgccgc cgagagaatg ggaatctcgg ttggcacggt
    10201 tgcgtattcg ccggacagcg ttgcggatta tgctttgatg ctgatgctga tggccatacg
    10261 gggtgcaaaa cccaccatgc acgccgtggc gcaacaagat ttcagattgg atcgtatccg
    10321 ggggaaagaa ctgggggata tgactgtggg agttattgga accggccata tcgggcaagc
    10381 ggtcgtcaaa aggctgcggg gatttggatg ccatgtgctg gcctatgata acagccgaaa
    10441 aatggatgca gattatgtcc agcttgatga gcttctaaaa aacagcgata ttgttacgct
    10501 ccatgtgccg ctttgtgcgg atacccgcca tctgatcggt cagaagcaaa ttggagagat
    10561 gaagcaaggc gcatttttaa tcaacaccgg gcgcggagcg cgtgtcgata ccggggcgct
    10621 ggtggaggcg ttggaaagcg gaaagctggg tggtgcggca ctggatgtgt tggagggcga
    10681 ggatcagttt gtttataccg attgctcgca gaaagtgctt gaccatccct ttttgtcgca
    10741 gcttttaagg atgccgaatg tgatcatcac accccatacg gcgtactaca ctgagcgtgt
    10801 gctgcaggat actacagaaa aaacaatcag gaattgtctc aattttgaaa ggagtttaca
    10861 gcatgaatag aataaaagtc gcaatcatct tcggcggttg ctcggaggaa catgatgtgt
    10921 cggtaaaagc cgcaatagaa attgctgcga acattgatac ggaaaaattc gatccgcact
    10981 acatcggaat tacaaaaaac ggtgtatgga agctatgcaa gaagccatgt acggaatggg
    11041 aagccgacag tctccccgcc atactctccc cggataggaa aacgcatggg ctgcttgtca
    11101 tgaaagaaag cgaatacgaa acacggcgta ttgatgtggc tttcccggtt ttgcatggca
    11161 aatgcgggga ggatggtgcg atacaggggc tgtttgtatt gtctggtatc ccctatgtgg
    11221 gctgtgatat tcaaagctcc gcagcttgca tggacaaatc actggcctac attcttacaa
    11281 aaaatgcggg catcgccgtt cccgaatttc aaatgattga taaaggtgac aagccggagg
    11341 cgggtgcgct tacctaccct gtctttgtga agccggcacg gtcaggttcg tcctttggcg
    11401 taaccaaagt aaacggtacg gaagaactta acgctgcgat agaagcggca ggacaatatg
    11461 atggaaaaat cttaattgag caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg
    11521 ggaacgagga tgatttgatt gtcggcgaag tggatcaaat ccggctgagc cacggtatct
    11581 tccgcatcca tcaggaaaac gagccggaaa aaggctcaga aaatgcgatg attacagttc
    11641 ccgcagacat tccggtcgag gaacgaaatc gggtgcagga aacggcaaag aaagtatatc
    11701 gggtgcttgg atgcagaggg cttgcccgtg ttgatctttt tttgcaggag gatggcggca
    11761 tcgttctaaa tgaggtcaat accctgcccg gttttacatc gtacagccgc tacccacgta
    11821 tgatggccgc cgcaggaatc acgcttcctg cactgattga cagcctgatt acattggcgt
    11881 taaagaggtg ataagtatgg aaaatggttt tttgttttta gatgaaatgt tgcatggcgt
    11941 ccgttgggat gccaagtacg ccacatggga taacttcaca ggaaaaccgg tggatgggta
    12001 tgaggtgaat cgcatcatcg gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat
    12061 ccatgcggct gcgcttggct atggcttgct tttatgggat ggatatcggc caagaactgc
    12121 ggtggactgc ttcctgcgtt gggcagcgca accggaggac aatctcacaa aagaaaaatt
    12181 ttaccccaat atagagcgag ccgagttgat tacaaagggt tatgtggcct cacaatccag
    12241 ccatagccgt ggaagcgcaa ttgatcttac gctctaccac ctggatacag gggaacttgt
    12301 ttcaatggga agtaacttcg attttatgga cgaacggtcg catcatacag caaaagggat
    12361 aggggatgca gaggcacaaa atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt
    12421 tcagtcttat cgctttgaat ggtggcacta taagttgatt gatgagccat accccgatac
    12481 ctattttaat tttgccgttt cataatgaaa gtattggttt tgtatcagat tttggatttg
    12541 ataaaatcta tgctggtcat ggacaatctt ttactggaaa gtggttaaag catcttttgt
    12601 aatacattgc aaattatagt attcaatctg cccagcggca gaaaagaaaa aaaccgctgt
    12661 tgggcagacc tttcccacgc ccattttgta ttttgctggc ggcggttttg agaaatcaag
    12721 gccgccgttc ttttctggca gcaaaaggtg tggcggccca taggaggatg ccgctatgct
    12781 gttttgctat tgccatgctc taatccactt ccaccactca aaaaaagcct tgcccctcca
    12841 tgaactgaac cccaaaagtt gaactattta atggactgt
//
LOCUS       DAZUKA010000401        12879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.085, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000401 DAZUKA010000000
VERSION     DAZUKA010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.085"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(371..607)
                     /locus_tag="ACG8PN_003188"
     CDS             complement(371..607)
                     /locus_tag="ACG8PN_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4863230.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(790..1161)
                     /locus_tag="ACG8PN_003189"
     CDS             complement(790..1161)
                     /locus_tag="ACG8PN_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4863231.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1158..2486)
                     /locus_tag="ACG8PN_003190"
     CDS             complement(1158..2486)
                     /locus_tag="ACG8PN_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4863232.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8PN_003191"
     CDS             complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8PN_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4863233.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3034..3404)
                     /locus_tag="ACG8PN_003192"
                     /pseudo
     CDS             complement(3034..3404)
                     /locus_tag="ACG8PN_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3549..3734
                     /locus_tag="ACG8PN_003193"
     CDS             3549..3734
                     /locus_tag="ACG8PN_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4863234.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3892..4059
                     /locus_tag="ACG8PN_003194"
     CDS             3892..4059
                     /locus_tag="ACG8PN_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863235.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4677..5336
                     /gene="vanR-B"
                     /locus_tag="ACG8PN_003195"
     CDS             4677..5336
                     /gene="vanR-B"
                     /locus_tag="ACG8PN_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4863236.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5339..6700
                     /gene="vanS-B"
                     /locus_tag="ACG8PN_003196"
     CDS             5339..6700
                     /gene="vanS-B"
                     /locus_tag="ACG8PN_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4863237.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6791..8092)
                     /locus_tag="ACG8PN_003197"
     CDS             complement(6791..8092)
                     /locus_tag="ACG8PN_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4863238.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            8371..9177
                     /locus_tag="ACG8PN_003198"
     CDS             8371..9177
                     /locus_tag="ACG8PN_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4863239.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9195..9902
                     /locus_tag="ACG8PN_003199"
     CDS             9195..9902
                     /locus_tag="ACG8PN_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFS4863240.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            9899..10870
                     /locus_tag="ACG8PN_003200"
     CDS             9899..10870
                     /locus_tag="ACG8PN_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4863241.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10863..11891
                     /gene="vanB"
                     /locus_tag="ACG8PN_003201"
     CDS             10863..11891
                     /gene="vanB"
                     /locus_tag="ACG8PN_003201"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4863242.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            11897..12505
                     /locus_tag="ACG8PN_003202"
     CDS             11897..12505
                     /locus_tag="ACG8PN_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4863243.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(12571..12744)
                     /locus_tag="ACG8PN_003203"
     CDS             complement(12571..12744)
                     /locus_tag="ACG8PN_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863244.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3411 a         2851 c         3221 g         3396 t
ORIGIN      
        1 ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca
       61 cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc
      121 gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga
      181 agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc
      241 cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg
      301 gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc
      361 ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg
      421 aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga
      481 tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg
      541 cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc
      601 cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg
      661 gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg
      721 gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata
      781 taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc
      841 ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt
      901 cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat
      961 ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag
     1021 cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt
     1081 gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta
     1141 attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa
     1201 atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg
     1261 tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg
     1321 tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac
     1381 aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat
     1441 tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata
     1501 tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg
     1561 atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa
     1621 ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc
     1681 gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct
     1741 ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag
     1801 gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata
     1861 aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa
     1921 tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc
     1981 cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc
     2041 acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc
     2101 tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga
     2161 ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc
     2221 acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg
     2281 gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc
     2341 agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc
     2401 ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg
     2461 ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt
     2521 cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc
     2581 cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat
     2641 agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca
     2701 ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc
     2761 gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac
     2821 aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca
     2881 ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac
     2941 tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc
     3001 agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt
     3061 cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc
     3121 ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat
     3181 ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac
     3241 cggcagactg ggaatggccc gtcgttttcg atgttggcaa gatagcgcca gtcaatattt
     3301 attttctctg ccattgcttt gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc
     3361 gcgccgaaag tttcaaagcc gggacaatct tcaatattcg ccatatcgca cctcccactt
     3421 acattgtata tttcatactc tgcccgtgga atgttggtat atgcaccttt cttaaagttt
     3481 atatttcaca ttttgtttct tttattcttc gagcaaaaga attataatgt accctgtgga
     3541 ggtgtactat ggactacatg acgttgaaag aggcaggcga gaaatggggt gtgcccccac
     3601 gatggataaa ctactattgt gctgcgggac gcatccccgg cgctgtaaag atggcgacga
     3661 tctggctcat tcccaaaact gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg
     3721 cggttactga atagtataat gaaaccggcg cattttggat atggttttga agagagagaa
     3781 agcatggtat tttgtcacgg aaaaactata agatgataga tggcaggctg ctccagaccg
     3841 ataaaaagta ttctcatctg aaacagaaag aaaagattta cacatggatg tatgaagaag
     3901 cgaaacttta tcatgacaga aatggaaaat gcccgaaaca aagacgagga agctgctgct
     3961 gtatcagccg tatatggtag aattgaaaag gccggcatct ggattcttta tggtgaagtc
     4021 tccagacatt ggataactgc tggtctaatg cgcctttgag tatggggtga ttgattttaa
     4081 taccggggag gtacggcttt ttgaggaaaa tattctggga tttacatttg gcgattatta
     4141 catttcctgt aactgcaagg acacggatga tgaagatatg aggaatatag acttgctgat
     4201 tttctactgt cttgaacggt attaggactg aatctgctgc gcggagtgcg gcaagagcga
     4261 ccggagggag tacacacagg ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc
     4321 aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc ccaacaagca aaacgagaac
     4381 gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag gatattctga tccattgctt
     4441 gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga aacatcccaa acattaagaa
     4501 ggtgttaatt attattatct tgatttaatt cgtttaaacc aataatatag atacatacac
     4561 catgaaaaga agcgagtatc ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg
     4621 aaactacagg gaaactacag actgttatgt tatcttctta aaatggaggg atttttatgt
     4681 cgatacgaat tctacttgtc gaggatgatg atcatatctg taatacagta agggcgtttc
     4741 tggctgaggc aggatatcag gtggatgcct gcacagatgg aaatgaggca tacaccaagt
     4801 tttacgaaaa cacttatcaa ctggttattc ttgatattat gctgcccggt atgaacgggc
     4861 atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc cattctgatg atgacagccc
     4921 tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa
     4981 agccattcaa gatgcagatt ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg
     5041 cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct gccggaggat ttcacggtac
     5101 tttgtgacgg tacagagctg cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg
     5161 tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt gtcacgtata tggggatatg
     5221 actttgaggg tgatggcagc acagtccaca ctcatatcaa aaatttgcgg gccaagctgc
     5281 cggaaaatat catcaaaacc attcgcggtg taggataccg attggaggaa tcataataat
     5341 ggaaagaaaa gggattttca ttaaggtttt ttcctatacg atcattgtcc tgctactgct
     5401 tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg tcttatttca gagtgatgga
     5461 attacagcaa acagtaaaat cctatcagcc attggtggaa ctgattcaga atagcgatag
     5521 gcttgatatt caagaggtgg cagggctgtt tcactacaat aaccaatcct ttgagtttta
     5581 tattgaagat aaagagggaa gcgtactcta tgccacacca aatgccaata catcaaatag
     5641 ttttagaccc aactttcttt atgtggtaca tagagatgat aatatttcga ttgttgctca
     5701 aagcaaggca ggtgtgggat tgctttatca agggctgaca attcggggaa ttgttatgat
     5761 tgcgataatg gttgtattca gccttttatg cgcgtatatc tttgcgcggc aaatgacaac
     5821 gccgatcaaa gccttagcgg acagtgcgaa taaaatggca aacctgaaag atgtaccgcc
     5881 gccgctggag cgaaaggatg agcttggcgc actggctcat gatatgcatt ccatgtatgt
     5941 caggctgaaa gaaaccatcg caaggctgga ggatgaaatc gcaagggaac atgagttgga
     6001 ggaaacacag cgatatttct ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc
     6061 tacaagcgtt ctgttggagg gaatgcttga aaatatcggt gactacaaag atcattctaa
     6121 gtatctgcgc gaatgcatca aaatgatgga taggcagggc aaaatcattt ccgaaatact
     6181 ggagcttgtc agcctgaatg atgggagaat cgtacccata gctgaaccgt tggacatagg
     6241 gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg
     6301 gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa
     6361 ggcactatcc aatgtcatat tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg
     6421 gatatggagt gagcctggtg ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca
     6481 cattgatgat actgcgcttc caaggctgtt caccccattc tatcgcattg atcaggcgcg
     6541 aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt
     6601 acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt
     6661 tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat
     6721 tttgggctct ttgtcaaata ggactgatag agtgagataa gaacatctaa ggtagaattc
     6781 cttttctact ttagatgttt tttgattcat aggtaatcag attatttagt aagaaaattc
     6841 tggtcttcat tttccgaaag cttcgaaagc cataagcgac tcgtttcaat gctttgatgt
     6901 gggtatgcag attttctatt ttggcatttg aataaggcaa ttcgagggct aaacgaatgc
     6961 cggattcata gtttataaaa gcttttttta tagaatgcag ttcttttgga acggaattgg
     7021 gcatcgttct tacaagttta aagaaagtgt ccgcctcttt gtcgtgaaac gcttgaagaa
     7081 tttcttgata gtaaaaatag gcgattcgta attcgtcaga gatcgaaagc agacgatcca
     7141 ccacttcact ttcggtgaga tacgcccaac caaaattgcg ccatttggtc catttgtcat
     7201 aaacaagctt gtcctgtcgt ttgatcagaa gccgagacaa cgcctttaat tgtcgataat
     7261 atttggcttg tgttggatcg tttcgatcaa agcttttcat gattcgtacg cgaatatcct
     7321 caaatttacg aatgagatgt ttgacaatgt gaaaacgatc cacaacgagt tttgcctttg
     7381 gaaaacattc ctttatcaga gaggcataag aaacattcat gtccgtcacg atgaccttta
     7441 ccttcaatcg tgcgcgtcgt tcaaattgca taaaataaga aacgagagcc tttttcttcc
     7501 gtgaaggaag aatatccagt aattttccag tatcgccatc catacaagaa aaggagtagg
     7561 gaccgacttg atttttcagt gaacgaaatt catccattaa taaacaacgc ggtaaccaat
     7621 ttctttttac ttttgtcatg gggatcagag aagcgagtgt ccgagaaacg gtcgaccaag
     7681 aaacgccgca atgcttagca attgtcgtca ttgcttgttt ttcagttagc tcttctaaaa
     7741 ttttatagcg cacttgttta gcgataaagc agtttctatc gacaatgtag gtattcgccg
     7801 taaaataggt gtggcagcca cgacagtggt agcgttgttt cgctaaagca agataagtca
     7861 gttggttcgc acatttatta agttgaatga gcgatttttt ctttccgttc ttaacgatca
     7921 tgccttcatg attcatctgt ttacacaaag gacagcgacg aatttcttta cttaacgtcg
     7981 cagaaacaac aagactcttg atattcttaa tgctcttcga ttccacattt gtgatgaaaa
     8041 tgtttttatc tttgattcca agtagtagtt tgatatgatg aagatcaggc atacagaacg
     8101 ctcctttgat tgtgttgtgg tgattcaatt ctaacggagc ttctgtatgt tttgtttttt
     8161 catgctcaaa agggaccgat agaatgattt tctatcagtc ctagaaatta tagagccgat
     8221 tttgaccgac aggtataacc ctgccggtct ttttttgctg ctacaggaaa actacagact
     8281 ggctacaggg aaagtacaga tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt
     8341 agttttattg caaaggaggc attcgatcaa atggaaaaaa gcaactatca ttccaatgcg
     8401 gatcatcaca aacggcatat gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc
     8461 attatttcgt tcacagtctg cacgctgttt ttggggtgga gactggcttc cgtattggag
     8521 gcaacacaga taccgcccat ccctgcaact catacaggca gcagcactga cgtagtggag
     8581 aatttggagg aaaacgctct tgccaccgcc aaagaacagg gagatgaaca ggaatggagc
     8641 ctgattttag tgaacaggca gaaccccatc cccgcacagt acgatgtgga gcttgagcaa
     8701 ctatcaaatg gtgagcggat agatattcgg atttctccct atcttcaaga tttgtttgat
     8761 gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg gataccgaac aacagaaaaa
     8821 cagcaagaaa ttatggatga aaaaattgcc gaatataagg cgaaaggcta cacctctgca
     8881 caggctaaag cggaagcaga aacttgggtg gccgtgccgg gaacgagcga gcatcagctt
     8941 ggtcttgctg tggatatcaa tgcggacgga attcattcaa caggcaacga ggtttataga
     9001 tggctggatg aaaacagcta tcgttttggt tttattcgtc gctacccacc agacaagaca
     9061 gagataaccg gtgtgagcaa cgagccgtgg cattaccgat atgttggcat cgaagctgcc
     9121 acagagatgt acaaccaagg ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa
     9181 aaggatgtta tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa
     9241 tgagacgggc gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct
     9301 atgcgcagac gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat
     9361 tatacaacca caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa
     9421 agctggcagc aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct
     9481 ggttgttcct gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt
     9541 ttccagagcc aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga
     9601 ttgatttaaa agtgcgaaac gataccgact gcacctacca aatatgggtg accttagatg
     9661 atgagaaaat catcggtcag gtgttcgccg acaaagagcc ccaagcatta tacaaaatta
     9721 caaatggcag tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta
     9781 aacggatgca agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa
     9841 acaaatgcaa aatctgttat cccctcccgg aaagtgtgga tattcaggag gagaaccaat
     9901 gagaaaaagt gtgggcatta ctgtttttgg atgcgaacag gatgaggcaa atgctttccg
     9961 cgctttatcg ccggattttc atattatccc tacgctgatt agcgatgcga tatcggcaga
    10021 caacgcaaaa ttggccgctg gcaatcaatg cgttagcgta agccataagt ccgaggtttc
    10081 cgaggcgaca attcttgcgc tgagaaaggt cggggtaaaa tacatttcta cccgcagcat
    10141 cggctgcgat cacattgata cgactgccgc cgagagaatg ggaatctcgg ttggcacggt
    10201 tgcgtattcg ccggacagcg ttgcggatta tgctttgatg ctgatgctga tggccatacg
    10261 gggtgcaaaa cccaccatgc acgccgtggc gcaacaagat ttcagattgg atcgtatccg
    10321 ggggaaagaa ctgggggata tgactgtggg agttattgga accggccata tcgggcaagc
    10381 ggtcgtcaaa aggctgcggg gatttggatg ccatgtgctg gcctatgata acagccgaaa
    10441 aatggatgca gattatgtcc agcttgatga gcttctaaaa aacagcgata ttgttacgct
    10501 ccatgtgccg ctttgtgcgg atacccgcca tctgatcggt cagaagcaaa ttggagagat
    10561 gaagcaaggc gcatttttaa tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct
    10621 ggtggaggcg ttggaaagcg gaaagctggg tggtgcggca ctggatgtgt tggagggcga
    10681 ggatcagttt gtttataccg attgctcgca gaaagtgctt gaccatccct ttttgtcgca
    10741 gcttttaagg atgccgaatg tgatcatcac accccatacg gcgtactaca ctgagcgtgt
    10801 gctgcaggat actacagaaa aaacaatcag gaattgtctc aattttgaaa ggagtttaca
    10861 gcatgaatag aataaaagtc gcaatcatct tcggcggttg ctcggaggaa catgatgtgt
    10921 cggtaaaagc cgcaatagaa attgctgcga acattgatac ggaaaaattc gatccgcact
    10981 acatcggaat tacaaaaaac ggtgtatgga agctatgcaa gaagccatgt acggaatggg
    11041 aagccgacag tctccccgcc atactctccc cggataggaa aacgcatggg ctgcttgtca
    11101 tgaaagaaag cgaatacgaa acacggcgta ttgatgtggc tttcccggtt ttgcatggca
    11161 aatgcgggga ggatggtgcg atacaggggc tgtttgtatt gtctggtatc ccctatgtgg
    11221 gctgtgatat tcaaagctcc gcagcttgca tggacaaatc actggcctac attcttacaa
    11281 aaaatgcggg catcgccgtt cccgaatttc aaatgattga taaaggtgac aagccggagg
    11341 cgggtgcgct tacctaccct gtctttgtga agccggcacg gtcaggttcg tcctttggcg
    11401 taaccaaagt aaacggtacg gaagaactta acgctgcgat agaagcggca ggacaatatg
    11461 atggaaaaat cttaattgag caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg
    11521 ggaacgagga tgatttgatt gtcggcgaag tggatcaaat ccggctgagc cacggtatct
    11581 tccgcatcca tcaggaaaac gagccggaaa aaggctcaga aaatgcgatg attacagttc
    11641 ccgcagacat tccggtcgag gaacgaaatc gggtgcagga aacggcaaag aaagtatatc
    11701 gggtgcttgg atgcagaggg cttgcccgtg ttgatctttt tttgcaggag gatggcggca
    11761 tcgttctaaa tgaggtcaat accctgcccg gttttacatc gtacagccgc tacccacgta
    11821 tgatggccgc cgcaggaatc acgcttcctg cactgattga cagcctgatt acattggcgt
    11881 taaagaggtg ataagtatgg aaaatggttt tttgttttta gatgaaatgt tgcatggcgt
    11941 ccgttgggat gccaagtacg ccacatggga taacttcaca ggaaaaccgg tggatgggta
    12001 tgaggtgaat cgcatcatcg gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat
    12061 ccatgcggct gcgcttggct atggcttgct tttatgggat ggatatcggc caagaactgc
    12121 ggtggactgc ttcctgcgtt gggcagcgca accggaggac aatctcacaa aagaaaaatt
    12181 ttaccccaat atagagcgag ccgagttgat tacaaagggt tatgtggcct cacaatccag
    12241 ccatagccgt ggaagcgcaa ttgatcttac gctctaccac ctggatacag gggaacttgt
    12301 ttcaatggga agtaacttcg attttatgga cgaacggtcg catcatacag caaaagggat
    12361 aggggatgca gaggcacaaa atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt
    12421 tcagtcttat cgctttgaat ggtggcacta taagttgatt gatgagccat accccgatac
    12481 ctattttaat tttgccgttt cataatgaaa gtattggttt tgtatcagat tttggatttg
    12541 ataaaatcta tgctggtcat ggacaatctt ttactggaaa gtggttaaag catcttttgt
    12601 aatacattgc aaattatagt attcaatctg cccagcggca gaaaagaaaa aaaccgctgt
    12661 tgggcagacc tttcccacgc ccattttgta ttttgctggc ggcggttttg agaaatcaag
    12721 gccgccgttc ttttctggca gcaaaaggtg tggcggccca taggaggatg ccgctatgct
    12781 gttttgctat tgccatgctc taatccactt ccaccactca aaaaaagcct tgcccctcca
    12841 tgaactgaac cccaaaagtt gaactattta atggactgt
//
LOCUS       DAZUKA010000402         5863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000402 DAZUKA010000000
VERSION     DAZUKA010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.158"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1024
                     /locus_tag="ACG8PN_003204"
     CDS             128..1024
                     /locus_tag="ACG8PN_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4863245.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1174..2115)
                     /locus_tag="ACG8PN_003205"
     CDS             complement(1174..2115)
                     /locus_tag="ACG8PN_003205"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4863246.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2241..3362
                     /locus_tag="ACG8PN_003206"
     CDS             2241..3362
                     /locus_tag="ACG8PN_003206"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4863247.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3620..5122
                     /gene="eat(A)"
                     /locus_tag="ACG8PN_003207"
     CDS             3620..5122
                     /gene="eat(A)"
                     /locus_tag="ACG8PN_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4863248.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5189..5716
                     /locus_tag="ACG8PN_003208"
     CDS             5189..5716
                     /locus_tag="ACG8PN_003208"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4863249.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1924 a         1031 c         1224 g         1684 t
ORIGIN      
        1 atttatttca tcatattttc atacaaatgt taaattttca tacaaatata ggaaacgttt
       61 tactggcttt ttactattta aacaggctat cataggaaaa agtgacagag aaagtgaggc
      121 ggaaaagatg aacaaaaatg acgtactgct agttgcaaaa aataaatcac tttatttttt
      181 gccttccatc cgctctttag atgtttggtc cggaaaagat ttactacagt cggaattcat
      241 taaatcagct agacaacagt tggcaaatta tcagctgatc gtttttttag attatggatt
      301 tgatatggag atggctcgac gcgtgcgccc atttaccaat gcaaaaattg tgttattttt
      361 ttggaatcac ttcaaagcag aacacgaggc gatgctgaag caggcacaag atgaaccggc
      421 aatcgatgag atttatcatt ttgatttttt ggaagcaaaa gaacttgggc tgaaacataa
      481 tagttcattt tattcgaaac atatgaattt ttcttctagc gaaccaaaga cggatctatt
      541 ttttggtgca acgaataatg ggagaaaaga acgagcagaa agctataagc aggaatttat
      601 aaaaagagat gttacgacca actatttcat tcttccacat cgagggaatg aacaacctgg
      661 atatcttact tatgaagagt acctgaaaaa aacaggggaa tcgcgcggga ttctggaact
      721 gttgcgggag ggtcagcaag gagtgacttt gcggacattt gaatccatgt attttcaaaa
      781 gaaactagtg acggataatc aagcgattgc tcattatcat ttttatcgtc ctgaaaatat
      841 ttttttgctt cgagaacgtg atttagatga attaccggaa tttctgcata caccatttaa
      901 gccaatccta tcagagctat tggaatttta tgatgcagaa aattgggcaa aacgatttat
      961 cactaacgat tccactattt atgagcaata cgaatatgac ctctcctctg ttgaaaacaa
     1021 ataaaaaacc tacattttga agagattatg tactgaaaca aacaagcaca attcttatgg
     1081 catcatgaga gttgtgtttt ctttttgatc ttttattggg cttaaaaaaa gagaaccagc
     1141 tgctgattca gctgattctc atcagagagc cttttattct gcgccaagga ttccttcttt
     1201 tgcatggact aattgggtca aaaaggcaca atatggtgta gcaatatcat atttttgtcc
     1261 ttttcgccaa atggcaccat tgatgtagtc gatttcagtc aaacgatggt ttttgatcag
     1321 gtcttggtac atagatgggt aatgcaaccc gatgttgtca ggatcataag tagaagcaat
     1381 atgttggtac acttcttctt gatctagtat gattccttct tttgctgcga tagaagcaaa
     1441 ttcagatacg attgttcgaa ccatgctctc tgccgctttt tgcgcgccta gttcagcaat
     1501 attacaatcc aaaatcgtgc ataaaccatt caatgttcca tttacacaag cttttcgcca
     1561 aatggaataa cgaacgttat ggctatagat cggatttaaa tttgcttctt gaaagacttc
     1621 tacaactttt tttgtaaatg cttcgccttc tggttccaag ttctctagtt cgatttctcc
     1681 atcacctaac aatgtgactt ttccgggacc agctagacca gctgtccaca tcgtgatccc
     1741 aaataagata tttttctttg gcacatattt ttccagtaca tcttcatgac ctaatccatt
     1801 tagtaaacac aaaacataag tattgtcagt aataatggat tggatcgaac aaaacatatc
     1861 atctaattga ttggcttttg ttaatgctac gatcaagtcc acgtgttcgt tgctttcgat
     1921 gatttcttct ggtgaataga ttggcagttt tgcgaccact tctttaccgt taaagtcagc
     1981 aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc caacgatcga tcaagaggac
     2041 ctcatttccg ctttgatgta gcatcaatcc gattcggctg cccattgctc cggccccagc
     2101 tattgcgatt ttcatgtttt ttctctcctt tattcaatga gttccctttt agtatataga
     2161 aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa
     2221 gaagaaagtg agggatacag atggatattt tggcggcaat tgattcattt aaaggaagtg
     2281 cttcttcatt tgaactaaat caggcagttc taaatggctg ggaagatcgt aaaggaagaa
     2341 aaatcaatgt gccgattgca gatggcggag aaaatactgc agaagcaatc tattacgcgc
     2401 tcaggggaac atggcgtacc attgatggga tggatctctt attccgtcag cgaaaaatca
     2461 gctatttatt gacaacttgg caaggaaaaa acaccgcagt catcgaatct gcacaagcat
     2521 tggggttaga tctacttgat aaaccaacgg atcaaactat ccgacttgct tccagttatg
     2581 ccttaggaga acttattaaa gatgcactgc ttcaagaagt agaacaaatt attgtcactt
     2641 taggaggaag cgggtgtagc gatggtggtc taggattact ccaatccttg ggtgcttctt
     2701 tagaagggta cacagaagga aatcctttat tatctactaa aaaacttcat tttgatcgaa
     2761 gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc agatgttacc aatccatata
     2821 caggtcaaca aggagctgct tggcaatttg gaagacaaaa aggaggaata gatacgacgc
     2881 tagctttctt agataaacaa gcaggaaaga ttgctcagca aataaaagat ttctgggagg
     2941 tggatttaga tgatattcca ggaagtggag cagcaggcgg tattggtgga gccctttttt
     3001 tgttgggagc gaagatggtt tctggtttcg atttagtcag ccaaatcact ggattggaaa
     3061 aaaagatcga acaagcagat gtcatcatta caggagaagg gcggatcgat cagcaaacga
     3121 tccacggaaa agttccatac ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa
     3181 tcgctatttg cggaagtaga gaccaagagc tagaaggact ttctgatttt ctcccaattg
     3241 tcttaagtat tcatttgggt ccaataggtt tagaagaagc aatggaacac aggcaaacat
     3301 tagaaaaaac aaaaatctta ggacagacac ttagcagatt tttcaccatc ctcccgaagt
     3361 agttgacaaa atcattttct ttatgctatc gtaatttcaa atataggaca aagtgaggga
     3421 tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga
     3481 gacaaggaag cagtgtgcag attttcggga ttggcttggt tttttcgatg tatgggtctt
     3541 tttgtgtgta tccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt
     3601 ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa aatctgacat tcggctacga
     3661 cagccaaggc acattattat ttgaacaagc aaatctaaat tttgacacac aatggaaact
     3721 aggacttatc ggacgaaacg gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa
     3781 actaccttat caagggcaag taatccatca gcaagaattt gcctatttcc cgcaacagac
     3841 aaaagataaa gaacgtttaa cctattacgt gttaaatgat attacggatt ttgagatatg
     3901 ggaaatcgaa agagagctcc aattgatgca aacagatcct gaaatcttat ggagagaatt
     3961 cagcacacta tcggggggag agaagacaaa agtcctactg gcacttttat ttgtggatga
     4021 cactcatttc ccgttaatcg atgaaccaac gaatcatttg gatatctctg gtagaaaaca
     4081 agtagcggct tatttgaaaa agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg
     4141 atttatcgat gaagtagtgg accatgtttt agcaatcgaa aaaagtcaac tggaacttta
     4201 tcaagggaat ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc
     4261 tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag aaaacagcag ctgaaaaagc
     4321 cgaatggtct cgttcccgag aaggagataa aacaaagaaa caagtcggat tcatcgatac
     4381 tgaatctaga cgagtgaata aaggagcagt gggtgctgat gctgcacgga cgatgaaacg
     4441 atccaaagca atcgtgaatc ggatggagac ccagatcagc gagaaagaaa aactattaaa
     4501 agatatcgaa tatatcgatt ctttgacgat gaatagccaa gcgtctcacc ataagcgact
     4561 tttaagcgta gaagatcttc aattagggta tgaaaatctg ttattcgagc caattcattt
     4621 tacaatcgag cctcatcagc gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc
     4681 cattatccat tatcttctgg gggcattcaa cggcaaggtt ataggagaaa aataccagcc
     4741 aaaacatctg agcattagtt atgcaagcca aaattatgaa gacaatcgag gaacgttggc
     4801 ggaatttgca gagaaaaacc aagtagacta ccaagcattt ttgaacaacc tccgaaagct
     4861 tgggatggaa agagatgttt ttcataacaa gatcgagcag atgagtatgg gccaacggaa
     4921 aaaagtggaa ttggctaaat ctttatcaca gccagctgaa ctatatacat gggatgaacc
     4981 attgaattat ttggatgtct tcaatcaaga acaattagaa caactgatct tgaacgtgaa
     5041 acctgccatg ttactagtgg aacatgatca aaccttcctg gataaagtat ctactgagat
     5101 tatttctctt gagagaatct aacccttgta atctcaagaa caacaaggta tggttaaggg
     5161 gaactttgcc aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac
     5221 ttggaacgca caaggattag tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca
     5281 gatgcaggca cagaaactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat
     5341 catccattcc cctcttcaac gagcaagaga tacagcacaa gcagttgctg atcgtctcag
     5401 cttgccgctg accgttgatg aacgattggt agaaatggat tttggggatt atgatggact
     5461 gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa
     5521 tggggagtca gtcttagatg tgtatgcacg gatcgttcct ttattgaaag aatgcatcga
     5581 agacgaagaa aatgtctatc tgttagtttg ccataatgcc ttgataagag tgatcaatgc
     5641 ttattttcat ccgatgccta atgaggggtt ctttacattc atggtagata atacagaatt
     5701 ggtttcttat gagtaattat gtttttgtgt tatttaaata aaatatttca aggcactgct
     5761 tttgcttagc agtgcctttt ttaggttatg acaattcttt tgataatata aactcaacct
     5821 ttagaaatga aatttaatta ttttaataaa agtattgaaa ttt
//
LOCUS       DAZUKA010000403         3796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.202, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000403 DAZUKA010000000
VERSION     DAZUKA010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.202"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACG8PN_003209"
     CDS             110..193
                     /locus_tag="ACG8PN_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4863250.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG8PN_003210"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG8PN_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4863251.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACG8PN_003211"
     CDS             complement(1318..2181)
                     /locus_tag="ACG8PN_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4863252.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACG8PN_003212"
     CDS             complement(2183..2455)
                     /locus_tag="ACG8PN_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4863253.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACG8PN_003213"
     CDS             complement(2472..2681)
                     /locus_tag="ACG8PN_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4863254.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACG8PN_003214"
     CDS             complement(2759..3655)
                     /locus_tag="ACG8PN_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4863255.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DAZUKA010000404         3072 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.227, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000404 DAZUKA010000000
VERSION     DAZUKA010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.227"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..700
                     /gene="dfrG"
                     /locus_tag="ACG8PN_003215"
     CDS             203..700
                     /gene="dfrG"
                     /locus_tag="ACG8PN_003215"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4863256.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(772..2724)
                     /locus_tag="ACG8PN_003216"
     CDS             complement(772..2724)
                     /locus_tag="ACG8PN_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4863257.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2731..2967)
                     /locus_tag="ACG8PN_003217"
     CDS             complement(2731..2967)
                     /locus_tag="ACG8PN_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863258.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          943 a          536 c          513 g         1080 t
ORIGIN      
        1 acataccaaa ttacatacaa aatgatttat aaaatgcaat tgaaaatgat aattgaatga
       61 aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac
      121 taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt
      181 agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag
      241 agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa
      301 aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag
      361 agccttacct gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg
      421 ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat
      481 ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta
      541 catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga
      601 ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta
      661 ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa
      721 gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa
      781 ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt
      841 caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat
      901 tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc
      961 accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat
     1021 aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc
     1081 cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa
     1141 tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc
     1201 ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg
     1261 tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg
     1321 tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg
     1381 atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga
     1441 gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca
     1501 atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct
     1561 attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga
     1621 tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag
     1681 atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc
     1741 ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata caactatata
     1801 cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg
     1861 gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt
     1921 tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca
     1981 ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac
     2041 tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg
     2101 ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca
     2161 ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt
     2221 gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac
     2281 atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga
     2341 taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta
     2401 cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc
     2461 ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca
     2521 gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa
     2581 cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct
     2641 atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat cttccttcgt
     2701 ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga tacccatttt
     2761 ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc
     2821 taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg catattcagc
     2881 gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag acttagcgtt
     2941 cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct tagtaaattg
     3001 taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt cgcattttta
     3061 tatacatttt aa
//
LOCUS       DAZUKA010000405         1388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.299, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000405 DAZUKA010000000
VERSION     DAZUKA010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.299"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1313
                     /gene="mef(H)"
                     /locus_tag="ACG8PN_003218"
                     /gene_synonym="mefH"
     CDS             75..1313
                     /gene="mef(H)"
                     /locus_tag="ACG8PN_003218"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4863259.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          422 a          196 c          240 g          530 t
ORIGIN      
        1 ctataaaaga gcagtttatt taaaccatta tataaaccga cggtctgttt gattaaataa
       61 aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt atacttgttg
      121 ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca ttaccgtact
      181 atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc tgctcttttt
      241 tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca aataaaagaa
      301 acattatggt catactagat tttgtaacag caatcataat tttagtattt tatttgtcat
      361 tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt tatggtattt
      421 ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca tcagataaac
      481 ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt atcggaccag
      541 ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt gttagtatta
      601 tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac caaaaaagac
      661 ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt tatacctttt
      721 tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt ttcaatttgg
      781 ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc
      841 cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta tgtggtggta
      901 ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc ttgttactta
      961 tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag
     1021 tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg
     1081 ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa
     1141 tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta
     1201 ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt
     1261 caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg
     1321 ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa
     1381 ggatagtg
//
LOCUS       DAZUKA010000406         1207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.310, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000406 DAZUKA010000000
VERSION     DAZUKA010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.310"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..396
                     /locus_tag="ACG8PN_003219"
                     /pseudo
     CDS             47..396
                     /locus_tag="ACG8PN_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(668..958)
                     /locus_tag="ACG8PN_003220"
     CDS             complement(668..958)
                     /locus_tag="ACG8PN_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4863260.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          381 a          234 c          183 g          409 t
ORIGIN      
        1 aagcattact taaagataac ttaaaagtta tcttttaaat acttaattgt taaatacctt
       61 atttgatgca aaagaaatct acgatagtca acaaaaaaat tttcattata tccagaaaaa
      121 tttttcttag ttaaggacct atggattgca gtaatgcaga attcgtcgaa taaactgcca
      181 aagacataca atcatattgc ttttaaaatt gacgaacaag atatagattc gtttgtctca
      241 aaaatccaaa tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct
      301 tcttcaattt acttttacga ctatgataat cacctcttcg agttacatac cggtactctc
      361 caggaacgtt taaaatccta caattccact acctgagact agtaatgact agtctctttt
      421 tatggcctct gatcccccca ttgagcgaat actaataggg cttcgaattg atctgttcaa
      481 tctttaagca atatccctca aatgagtttg gaaagtttag tgattcatca caaatactta
      541 aatacatgta gttatatata gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa
      601 tcccatcgtc tgcatccatc tttccaaaac aagcggctag gacatttttg tctcagccgc
      661 ttgttttcta gtttattctt tcatcttgtc ggaccttttt tcttgggtat agacttttct
      721 catattctac cacaattttg ggtttcccta acacatgata ggatctcaca acaaattgct
      781 ttctattatt ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga
      841 agttcaaact atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg
      901 gtctggcact aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa
      961 ccttcacctc aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt
     1021 tatctttttg actttttgtc tattaccttt cttaaaatca tattggcact tcctgcgaag
     1081 atgaaggaca tgtactaaaa aaaagaggca gcagtataga tttaagtagt tcagttttgg
     1141 agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact
     1201 cgttttt
//
LOCUS       DAZUKA010000407          761 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5337
            SAMEA115707649-rid22576713.guided.349, whole genome shotgun
            sequence.
ACCESSION   DAZUKA010000407 DAZUKA010000000
VERSION     DAZUKA010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707649
            Sequence Read Archive: ERR13197377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288103.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288103.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:10:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,221
            CDSs (total)                      :: 3,160
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 38 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 10 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707649-rid22576713.guided.349"
                     /isolate="VRE5337"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..696
                     /gene="aac(6')"
                     /locus_tag="ACG8PN_003221"
     CDS             148..696
                     /gene="aac(6')"
                     /locus_tag="ACG8PN_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4863261.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          277 a          111 c          169 g          204 t
ORIGIN      
        1 aagtgtttct gtataatttt ttattttata agtaagataa ataatgaaag aatagagata
       61 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
      121 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
      181 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
      241 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
      301 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
      361 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
      421 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
      481 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
      541 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
      601 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
      661 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
      721 atcagcttca agaaataaga aggaatttac gaaaattact t
//
